Kuzmanović, S., Starović, M., Ristić, D., Vučurović, A., Kuzmanović, N., Trkulja, N.,& Aleksić, G.. (2024). Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе. in Институт за заштиту биља и животну средину
Институт за заштиту биља и животну средину..

Kuzmanović S, Starović M, Ristić D, Vučurović A, Kuzmanović N, Trkulja N, Aleksić G. Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе. in Институт за заштиту биља и животну средину. 2024;..

Kuzmanović, Slobodan, Starović, Mira, Ristić, Danijela, Vučurović, Ana, Kuzmanović, Nemanja, Trkulja, Nenad, Aleksić, Goran, "Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе" in Институт за заштиту биља и животну средину (2024).

TY  - CONF
AU  - Zečević, Katarina
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Krstić, Branka
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1207
C3  - XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina
T1  - FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA
ER  - 

@conference{
author = "Zečević, Katarina and Stanković, Ivana and Ristić, Danijela and Vučurović, Ivan and Krstić, Branka",
year = "2023",
journal = "XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina",
title = "FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA"
}

Zečević, K., Stanković, I., Ristić, D., Vučurović, I.,& Krstić, B.. (2023). FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA. in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina.

Zečević K, Stanković I, Ristić D, Vučurović I, Krstić B. FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA. in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina. 2023;..

Zečević, Katarina, Stanković, Ivana, Ristić, Danijela, Vučurović, Ivan, Krstić, Branka, "FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA" in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina (2023).

TY  - CONF
AU  - Keserović, Vukašin
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1206
AB  - Didymella pinodella – prozrokovač crne pegavosti graška je najrazornija bolest graška
širom sveta koja, u zavisnosti od uslova sredine i kvaliteta graška, može usloviti gubitke
prinosa od 28 do 88%.
U ovom radu, u in vitro uslovima, ispitan je uticaj fungicida različitog mehanizma
delovanja na izolate D. pinodella poreklom sa graška.
Izolati D. pinodella korišćeni u ispitivanjima, poreklom su sa lista i mahuna graška,
kolekcionisani tokom 2023 godine iz lokaliteta Trbušac i Skupljen (okolina Šapca) i deo su
kolekcije fitopatogenih gljiva Instituta za zaštitu bilja i životnu sredinu (Beograd). Ispitivana je
osetljivost gljive prema četiri kombinacije komercijalnih fungicida: ciprodinil + fludioksonil,
fluksapiroksad + difenokonazol, azoksistrobin + difenokonazol i difenokonazol a kao standard
je korišćen azoksistrobin. Primenjeni su fungicidi u početnoj preporučenoj koncentraciji
primene: ciprodinil + fludioksonil (25μg/10ml vode), fluksapiroksad + difenokonazol
(100μl/10ml vode), azoksistrobin + difenokonazol (50μl/10ml vode), difenokonazol
(25μl/10ml vode) i azoksistrobin (50μl/10ml vode). Za ispitivanje antifungalne aktivnosti
fungicida korišćena je mikrodiluciona metoda na miktrotitarskim pločama. Određivanje
minimalnih inhibitornih koncentracija (MIC) vršeno je pomoću skale razređenja primenjenih
fungicida od 10-1 do 10-9 u krompir dekstroznom tečnom medijumu u četiri ponavljanja. Na
kraju je dodavano 10 μl suspenzije micelije u tečni medijum finalne zapremine od 100 μl.
Mikrotitarske pločice inkubirane su u termostatu na 25°C u trajanju od 14 dana. Najniža
koncentracija na kojoj nije bilo rasta micelije uzimana je za minimalnu inhibitornu
koncentraciju (MIC).
Najviši stepen tokstičnosti ispoljila je kombinacija azoksistrobina i difenokonazola, sa
prosečnom vrednosti minimalne koncentracije inhibicije od 0,003 μl/ml; zatim kombinacija
ciprodinila i fludioksonila - MIC 0,02 μg/ml; kombinacija fluksapiroksada i difenokonazola -
MIC 0,08 μl/ml, dok je najnižu toksičnost ispoljio difenokonazol - MIC 0,2 μl/ml.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška
SP  - 84
ER  - 

@conference{
author = "Keserović, Vukašin and Starović, Mira and Ristić, Danijela and Vučurović, Ivan and Blagojević, Jovana and Aleksic, Goran",
year = "2023",
abstract = "Didymella pinodella – prozrokovač crne pegavosti graška je najrazornija bolest graška
širom sveta koja, u zavisnosti od uslova sredine i kvaliteta graška, može usloviti gubitke
prinosa od 28 do 88%.
U ovom radu, u in vitro uslovima, ispitan je uticaj fungicida različitog mehanizma
delovanja na izolate D. pinodella poreklom sa graška.
Izolati D. pinodella korišćeni u ispitivanjima, poreklom su sa lista i mahuna graška,
kolekcionisani tokom 2023 godine iz lokaliteta Trbušac i Skupljen (okolina Šapca) i deo su
kolekcije fitopatogenih gljiva Instituta za zaštitu bilja i životnu sredinu (Beograd). Ispitivana je
osetljivost gljive prema četiri kombinacije komercijalnih fungicida: ciprodinil + fludioksonil,
fluksapiroksad + difenokonazol, azoksistrobin + difenokonazol i difenokonazol a kao standard
je korišćen azoksistrobin. Primenjeni su fungicidi u početnoj preporučenoj koncentraciji
primene: ciprodinil + fludioksonil (25μg/10ml vode), fluksapiroksad + difenokonazol
(100μl/10ml vode), azoksistrobin + difenokonazol (50μl/10ml vode), difenokonazol
(25μl/10ml vode) i azoksistrobin (50μl/10ml vode). Za ispitivanje antifungalne aktivnosti
fungicida korišćena je mikrodiluciona metoda na miktrotitarskim pločama. Određivanje
minimalnih inhibitornih koncentracija (MIC) vršeno je pomoću skale razređenja primenjenih
fungicida od 10-1 do 10-9 u krompir dekstroznom tečnom medijumu u četiri ponavljanja. Na
kraju je dodavano 10 μl suspenzije micelije u tečni medijum finalne zapremine od 100 μl.
Mikrotitarske pločice inkubirane su u termostatu na 25°C u trajanju od 14 dana. Najniža
koncentracija na kojoj nije bilo rasta micelije uzimana je za minimalnu inhibitornu
koncentraciju (MIC).
Najviši stepen tokstičnosti ispoljila je kombinacija azoksistrobina i difenokonazola, sa
prosečnom vrednosti minimalne koncentracije inhibicije od 0,003 μl/ml; zatim kombinacija
ciprodinila i fludioksonila - MIC 0,02 μg/ml; kombinacija fluksapiroksada i difenokonazola -
MIC 0,08 μl/ml, dok je najnižu toksičnost ispoljio difenokonazol - MIC 0,2 μl/ml.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška",
pages = "84"
}

Keserović, V., Starović, M., Ristić, D., Vučurović, I., Blagojević, J.,& Aleksic, G.. (2023). Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 84.

Keserović V, Starović M, Ristić D, Vučurović I, Blagojević J, Aleksic G. Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:84..

Keserović, Vukašin, Starović, Mira, Ristić, Danijela, Vučurović, Ivan, Blagojević, Jovana, Aleksic, Goran, "Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):84.

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Trkulja, Nenad
AU  - Ristić, Danijela
AU  - Iličić, Renata
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1197
AB  - Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
T1  - Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe
SP  - 49
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Trkulja, Nenad and Ristić, Danijela and Iličić, Renata and Trkulja, Vojislav and Jelušić, Aleksandra",
year = "2023",
abstract = "Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova",
title = "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe",
pages = "49"
}

Popović Milovanović, T., Trkulja, N., Ristić, D., Iličić, R., Trkulja, V.,& Jelušić, A.. (2023). Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 49.

Popović Milovanović T, Trkulja N, Ristić D, Iličić R, Trkulja V, Jelušić A. Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova. 2023;:49..

Popović Milovanović, Tatjana, Trkulja, Nenad, Ristić, Danijela, Iličić, Renata, Trkulja, Vojislav, Jelušić, Aleksandra, "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe" in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova (2023):49.

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Starović, Mira
AU  - Ristić, Danijela
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1180
AB  - Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.
PB  - The American Phytopathological Society
T2  - Phytopathology
T1  - Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia
DO  - https://doi.org/10.1094/PHYTO-04-23-0133-R
ER  - 

@article{
author = "Blagojević, Jovana and Aleksic, Goran and Vučurović, Ivan and Starović, Mira and Ristić, Danijela",
year = "2023",
abstract = "Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.",
publisher = "The American Phytopathological Society",
journal = "Phytopathology",
title = "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia",
doi = "https://doi.org/10.1094/PHYTO-04-23-0133-R"
}

Blagojević, J., Aleksic, G., Vučurović, I., Starović, M.,& Ristić, D.. (2023). Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology
The American Phytopathological Society..
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R

Blagojević J, Aleksic G, Vučurović I, Starović M, Ristić D. Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology. 2023;.
doi:https://doi.org/10.1094/PHYTO-04-23-0133-R .

Blagojević, Jovana, Aleksic, Goran, Vučurović, Ivan, Starović, Mira, Ristić, Danijela, "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia" in Phytopathology (2023),
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R . .

Ristić, D., Vučurović, I., Starović, M., Aleksic, G., Štrbanović, R., Đurović, S.,& Nikolić, B.. (2022). Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Ristić D, Vučurović I, Starović M, Aleksic G, Štrbanović R, Đurović S, Nikolić B. Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika. in Institut za zaštitu bilja i životnu sredinu. 2022;..

Ristić, Danijela, Vučurović, Ivan, Starović, Mira, Aleksic, Goran, Štrbanović, Ratibor, Đurović, Sanja, Nikolić, Bogdan, "Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika" in Institut za zaštitu bilja i životnu sredinu (2022).

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Petrović, Tamaš
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Dudić, Dragana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/978
AB  - Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology

Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile, pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides their high changeability, another major reason is their ability to mimic host processes upon entering the host. Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness. Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+) methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal pattern in concordance with their similar genome organization? Among numerous different methylation patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well. This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.
PB  - Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology
C3  - Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia
T1  - Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses
SP  - 29
ER  - 

@conference{
author = "Banović Đeri, Bojana and Vidanović, Dejan and Tešović, Bojana and Petrović, Tamaš and Ristić, Danijela and Vučurović, Ivan and Dudić, Dragana",
year = "2021",
abstract = "Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology

Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile, pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides their high changeability, another major reason is their ability to mimic host processes upon entering the host. Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness. Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+) methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal pattern in concordance with their similar genome organization? Among numerous different methylation patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well. This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.",
publisher = "Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology",
journal = "Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia",
title = "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses",
pages = "29"
}

Banović Đeri, B., Vidanović, D., Tešović, B., Petrović, T., Ristić, D., Vučurović, I.,& Dudić, D.. (2021). Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia
Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology., 29.

Banović Đeri B, Vidanović D, Tešović B, Petrović T, Ristić D, Vučurović I, Dudić D. Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia. 2021;:29..

Banović Đeri, Bojana, Vidanović, Dejan, Tešović, Bojana, Petrović, Tamaš, Ristić, Danijela, Vučurović, Ivan, Dudić, Dragana, "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses" in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia (2021):29.

Zivkovic, S., Ristić, D.,& Stošić, S.. (2021). First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease
American Phytopathological Society., 105(8), 2246-2246.
https://doi.org/10.1094/PDIS-02-21-0323-PDN

Zivkovic S, Ristić D, Stošić S. First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease. 2021;105(8):2246-2246.
doi:10.1094/PDIS-02-21-0323-PDN .

Zivkovic, Svetlana, Ristić, Danijela, Stošić, Stefan, "First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia" in Plant Disease, 105, no. 8 (2021):2246-2246,
https://doi.org/10.1094/PDIS-02-21-0323-PDN . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - JOUR
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - MÖZCAN, Mehmet
AU  - Jošić, Dragana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/619
AB  - Leaf spot disease caused by Cercospora beticola Sacc. is the most destructive foliar disease of beet. Cercospora leaf spot is controlled primarily by fungicides because the non-chemical alternatives do not provide commercially viable control. One of the ways of reducing chemical application is the use of different essential oils (EOs) or antagonistic plant growth-promoting rhizobacteria (PGPB). This study evaluates several EOs and PGPB belonging to Pseudomonas chlororaphis as possible control agents of this pathogen. Antifungal properties were determined by in vitro microdilution method against five C. beticola monosporial isolates originated from the locality Brus, Serbia (53°53’ N, 21°04’E and 429 m above sea level) using EOs from medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum), and myrtle (Myrtus communis) obtained by a hydro-distillation method. All tested oils displayed some antifungal activity against the fungal isolates. Origanum vulgare EO demonstrated the strongest antifungal activity (MIC - 0.0055±0.0051mg/mL), Ocimum basilicum slightly lower (MIC - 0.075±0.045mg/mL), followed by Myrtus communis (MIC - 0.775±0.045 mg/ mL) and Echinophora tenuifolia (MIC - 7.75±4.5 mg/mL). Five tested P. chlororaphis strains exhibited some antagonistic effect against C. beticola. Overnight culture (ONC) of P. chlororaphis strain E65 induced the highest percentage of inhibition (75.8%), followed by N3 (72.0%). A cell-free supernatant (CFS) and the CFS treated with EDTA (CFS-EDTA) of these strains showed similar inhibition of 60.2 and 56.0%, and both strains suppressed C. beticola growth. P. chlororaphis strains M1 and K113 also reduced the fungal growth by 67-70% using ONC and between 48-57% using different CFS fractions. The strains L1 and B25 caused inhibition of 60% using ONC and 50% by CFS. The lowest inhibition (~40%) by CFS-EDTA and heat-treated cell-free supernatant (HT-CFS) was recorded for B25, which was used as a reference strain. The tested isolates of C. beticola were susceptible to all selected essential oils and P. chlororaphis strains E25, N3, M1, and K113 in vitro, making them a promising non-chemical control agent. It is recommended that these findings should be tested in field conditions.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.
EP  - 19
SP  - 9
VL  - 140
DO  - 10.2298/ZMSPN2140009S
ER  - 

@article{
author = "Starović, Mira and Ristić, Danijela and Pavlović, Snežana and MÖZCAN, Mehmet and Jošić, Dragana",
year = "2021",
abstract = "Leaf spot disease caused by Cercospora beticola Sacc. is the most destructive foliar disease of beet. Cercospora leaf spot is controlled primarily by fungicides because the non-chemical alternatives do not provide commercially viable control. One of the ways of reducing chemical application is the use of different essential oils (EOs) or antagonistic plant growth-promoting rhizobacteria (PGPB). This study evaluates several EOs and PGPB belonging to Pseudomonas chlororaphis as possible control agents of this pathogen. Antifungal properties were determined by in vitro microdilution method against five C. beticola monosporial isolates originated from the locality Brus, Serbia (53°53’ N, 21°04’E and 429 m above sea level) using EOs from medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum), and myrtle (Myrtus communis) obtained by a hydro-distillation method. All tested oils displayed some antifungal activity against the fungal isolates. Origanum vulgare EO demonstrated the strongest antifungal activity (MIC - 0.0055±0.0051mg/mL), Ocimum basilicum slightly lower (MIC - 0.075±0.045mg/mL), followed by Myrtus communis (MIC - 0.775±0.045 mg/ mL) and Echinophora tenuifolia (MIC - 7.75±4.5 mg/mL). Five tested P. chlororaphis strains exhibited some antagonistic effect against C. beticola. Overnight culture (ONC) of P. chlororaphis strain E65 induced the highest percentage of inhibition (75.8%), followed by N3 (72.0%). A cell-free supernatant (CFS) and the CFS treated with EDTA (CFS-EDTA) of these strains showed similar inhibition of 60.2 and 56.0%, and both strains suppressed C. beticola growth. P. chlororaphis strains M1 and K113 also reduced the fungal growth by 67-70% using ONC and between 48-57% using different CFS fractions. The strains L1 and B25 caused inhibition of 60% using ONC and 50% by CFS. The lowest inhibition (~40%) by CFS-EDTA and heat-treated cell-free supernatant (HT-CFS) was recorded for B25, which was used as a reference strain. The tested isolates of C. beticola were susceptible to all selected essential oils and P. chlororaphis strains E25, N3, M1, and K113 in vitro, making them a promising non-chemical control agent. It is recommended that these findings should be tested in field conditions.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.",
pages = "19-9",
volume = "140",
doi = "10.2298/ZMSPN2140009S"
}

Starović, M., Ristić, D., Pavlović, S., MÖZCAN, M.,& Jošić, D.. (2021). Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 9-19.
https://doi.org/10.2298/ZMSPN2140009S

Starović M, Ristić D, Pavlović S, MÖZCAN M, Jošić D. Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.. in Zbornik Matice srpske za prirodne nauke. 2021;140:9-19.
doi:10.2298/ZMSPN2140009S .

Starović, Mira, Ristić, Danijela, Pavlović, Snežana, MÖZCAN, Mehmet, Jošić, Dragana, "Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc." in Zbornik Matice srpske za prirodne nauke, 140 (2021):9-19,
https://doi.org/10.2298/ZMSPN2140009S . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Danijela
AU  - Vucurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/594
AB  - A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia
EP  - 797
IS  - 3
SP  - 787
VL  - 102
DO  - 10.1007/s42161-020-00504-7
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Danijela and Vucurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia",
pages = "797-787",
number = "3",
volume = "102",
doi = "10.1007/s42161-020-00504-7"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, D., Vucurović, I.,& Krstić, B.. (2020). Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology
Springer, New York., 102(3), 787-797.
https://doi.org/10.1007/s42161-020-00504-7

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić D, Vucurović I, Krstić B. Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology. 2020;102(3):787-797.
doi:10.1007/s42161-020-00504-7 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Danijela, Vucurović, Ivan, Krstić, Branka, "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia" in Journal of Plant Pathology, 102, no. 3 (2020):787-797,
https://doi.org/10.1007/s42161-020-00504-7 . .

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/917
AB  - Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.
PB  - Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi
EP  - 88
SP  - 79
VL  - 136
DO  - 10.2298/zmspn1936079z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš",
year = "2019",
abstract = "Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.",
publisher = "Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi",
pages = "88-79",
volume = "136",
doi = "10.2298/zmspn1936079z"
}

Zivkovic, S., Stošić, S., Ristić, D., Vučurović, I.,& Stevanović, M.. (2019). Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke
Matica srpska., 136, 79-88.
https://doi.org/10.2298/zmspn1936079z

Zivkovic S, Stošić S, Ristić D, Vučurović I, Stevanović M. Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke. 2019;136:79-88.
doi:10.2298/zmspn1936079z .

Zivkovic, Svetlana, Stošić, Stefan, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi" in Zbornik Matice srpske za prirodne nauke, 136 (2019):79-88,
https://doi.org/10.2298/zmspn1936079z . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Pfaf-Dolovac, Erika
AU  - Aleksić, Goran
AU  - Vučurović, Ana
AU  - Starović, Mira
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/560
AB  - It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
PB  - Springer, Dordrecht
T2  - Potato Research
T1  - The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops
EP  - 46
IS  - 1
SP  - 31
VL  - 62
DO  - 10.1007/s11540-018-9395-y
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Pfaf-Dolovac, Erika and Aleksić, Goran and Vučurović, Ana and Starović, Mira",
year = "2019",
abstract = "It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.",
publisher = "Springer, Dordrecht",
journal = "Potato Research",
title = "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops",
pages = "46-31",
number = "1",
volume = "62",
doi = "10.1007/s11540-018-9395-y"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Pfaf-Dolovac, E., Aleksić, G., Vučurović, A.,& Starović, M.. (2019). The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research
Springer, Dordrecht., 62(1), 31-46.
https://doi.org/10.1007/s11540-018-9395-y

Ristić D, Vučurović I, Kuzmanović S, Pfaf-Dolovac E, Aleksić G, Vučurović A, Starović M. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research. 2019;62(1):31-46.
doi:10.1007/s11540-018-9395-y .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Pfaf-Dolovac, Erika, Aleksić, Goran, Vučurović, Ana, Starović, Mira, "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops" in Potato Research, 62, no. 1 (2019):31-46,
https://doi.org/10.1007/s11540-018-9395-y . .

TY  - CONF
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Ristić, Danijela
AU  - Marković, Jordan
AU  - Jovanović, Snežana
AU  - Tabaković, Marijenka
AU  - Stanisavljević, Rade
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1148
AB  - Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke starosti pet godina u klijalistu na temperaturi od 20o C u mraku, deset dana (prema Pravilniku o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta (P≤0,05).
PB  - Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi
C3  - Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
T1  - Uticaj starosti semena na kvalitet različitih sorata lucerke
SP  - 115
ER  - 

@conference{
author = "Štrbanović, Ratibor and Poštić, Dobrivoj and Ristić, Danijela and Marković, Jordan and Jovanović, Snežana and Tabaković, Marijenka and Stanisavljević, Rade",
year = "2018",
abstract = "Plava lucerka (Medicago sativa L.) je zbog svih svojih osobina najvažnija krmna biljka u našoj zemlji. Veliki značaj lucerke se ogleda u proizvodnji kvalitetne kabaste hrane, ali je i seme lucerke vredna i kurentna roba na domaćem i inostranom tržištu. Areal gajenja lucerke je na svim kontinentima u više od 80 zemalja, od umereno hladnog do tropskog pojasa. Široka geografska rasprostranjenost lucerke uslovljena je njenom velikom adaptabilnošću na različite klimatske i zemljišne uslove. Zasnivanje useva krmnih biljaka vrši se direktnom setvom semena. Obzirom na te okolnosti, brza i uniformna pojava snaznih klijanaca lucerke je veoma značajna kako bi se osigurala visoka uniformnost klijanaca, što utiče na početnu fazu razvoja useva, a kao rezultat svega je visok i stabilan prinos. Sve ovo ukazuje na važnost izbora semena dobrog kvaliteta.U ovom radu je ispitivan uticaj starosti semena na kvalitet tri različite sorte lucerke (Kruševačka 28, NS-Banat i Čačanka-10). Seme ispitivanih sorata lucerke čuvano je pet godina u papirnim kesama u kontrolisanim uslovima temperature i relativne vlažnosti vazduha. Zatim je ispitivana klijavost semena različitih sorata lucerke starosti pet godina u klijalistu na temperaturi od 20o C u mraku, deset dana (prema Pravilniku o ispitivanju kvaliteta semena poljoprivrednog bilja 47/87). Dobijeni rezultati ukazuju da je starost semena značajno uticala na ukupnu klijavost, nenormalne klijance, tvrdo i mrtvo seme. Najveća klijavost je ostvarena iz semena starog dve godine, pri tome uticaj sorte je takodje statistički značajno delovao na ukupnu klijavost, kao i interakcija starost semena x sorta (P≤0,05).",
publisher = "Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda",
title = "Uticaj starosti semena na kvalitet različitih sorata lucerke",
pages = "115"
}

Štrbanović, R., Poštić, D., Ristić, D., Marković, J., Jovanović, S., Tabaković, M.,& Stanisavljević, R.. (2018). Uticaj starosti semena na kvalitet različitih sorata lucerke. in Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda
Novi Sad : Društvo za procesnu tehniku i energetiku u poljoprivredi., 115.

Štrbanović R, Poštić D, Ristić D, Marković J, Jovanović S, Tabaković M, Stanisavljević R. Uticaj starosti semena na kvalitet različitih sorata lucerke. in Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda. 2018;:115..

Štrbanović, Ratibor, Poštić, Dobrivoj, Ristić, Danijela, Marković, Jordan, Jovanović, Snežana, Tabaković, Marijenka, Stanisavljević, Rade, "Uticaj starosti semena na kvalitet različitih sorata lucerke" in Nacionalna konferencija sa međunarodnim učešćem Procesna Tehnika i Energetika u Poljoprivredi - PTEP 2018, 15-20.04, Brzeće - Zbornik izvoda (2018):115.

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Krstić, Branka
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1025
AB  - Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine
T2  - Biljni lekar
T1  - Kompleks virusa prouzrokovača uvijenosti lišća vinove loze
EP  - 690
IS  - 6
IS  - 681
VL  - 46
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Krstić, Branka",
year = "2018",
abstract = "Brojnost virusa infektivnih za vinovu lozu stalno se povećava, tako da je do sada opisano više od 60 vrsta koji mogu da zaraze vinovu lozu. Paralelno sa globalnom ekspanzijom vinogradarske i vinarske industrije zapaženo je povećanje učestalosti i ekonomskog značaja oboljenja uvijenosti lišća vinove loze (Grapevine Leafroll Disease, GLD). GLD, osim što smanjuje vitalnost i dugovečnost vinove loze, značajno smanjuje prinos i kvalitet grožđa. Epidemiologija oboljenja je nedovoljno istražena zbog kompleksnosti ove bolesti, genetički i serološki različitih vrsta unutar kompleksa i različitosti simptoma kod crvenih i belih sorti vinove loze. Кompleks virusa prouzrokovača uvijenosti lišća vinove loze (Grapevine leafrollassociated viruses, GLRaVs) čini pet različitih virusa koji pripadaju familiji Closteroviridae: jedna vrsta koja pripada rodu Closterovirus (GLRaV-2), tri rodu Ampelovirus (GLRaV-1, GLRaV -3, GLRaV -4, i njegovi sojevi: GLRaV-4 soj 5, GLRaV-4 soj 6, GLRaV-4 soj 9, GLRaV-4 soj Pr, GLRaV-4 soj De i GLRaV-4 soj Car) i jedna vrsta iz roda Velarivirus (GLRaV-7). GLRaVs se prenose kalemljenjem i propagativnim materijalom (podloga i vijoka), a neke vrste i štitastim vašima. Virusi koji izazivaju uvijenost lišća se obično javljaju u mešanoj infekciji, a često su prisutni i u mešanoj infekciji sa drugim virusima vinove loze. Ranija istraživanja sprovedena u vinogorjima u Srbiji utvrdila su prisustvo GLRV-1, -2 i -3, pri čemu je GLRaV-3 bio najzastupljeniji. Ukoliko je širenje virusa unutar zasada putem vektora izraženo, krčenje ili uklanjanje zaraženih čokota, kontrola štitastih vaši i adekvatne sanitarne mere mogu redukovati nove infekcije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine",
journal = "Biljni lekar",
title = "Kompleks virusa prouzrokovača uvijenosti lišća vinove loze",
pages = "690",
number = "6, 681",
volume = "46"
}

Ristić, D., Vučurović, I., Stanković, I., Vučurović, A., Zečević, K.,& Krstić, B.. (2018). Kompleks virusa prouzrokovača uvijenosti lišća vinove loze. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine., 46(6).

Ristić D, Vučurović I, Stanković I, Vučurović A, Zečević K, Krstić B. Kompleks virusa prouzrokovača uvijenosti lišća vinove loze. in Biljni lekar. 2018;46(6):null-690..

Ristić, Danijela, Vučurović, Ivan, Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Krstić, Branka, "Kompleks virusa prouzrokovača uvijenosti lišća vinove loze" in Biljni lekar, 46, no. 6 (2018).

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 

@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}

Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z

Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .

Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .

Aleksic, G., Kuzmanović, S., Zivkovic, S., Popović Milovanović, T., Ristić, D., Stevanović, M.,& Borić, B.. (2017). Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Aleksic G, Kuzmanović S, Zivkovic S, Popović Milovanović T, Ristić D, Stevanović M, Borić B. Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke. in Institut za zaštitu bilja i životnu sredinu. 2017;..

Aleksic, Goran, Kuzmanović, Slobodan, Zivkovic, Svetlana, Popović Milovanović, Tatjana, Ristić, Danijela, Stevanović, Miloš, Borić, Borislav, "Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke" in Institut za zaštitu bilja i životnu sredinu (2017).

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Gašić, Katarina
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Ristić, Danijela
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1013
AB  - Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.
PB  - Herbološko društvo Srbije
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi
EP  - 343
SP  - 331
VL  - 133
DO  - 10.2298/ZMSPN1733331Z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Stevanović, Miloš and Gašić, Katarina and Aleksic, Goran and Vučurović, Ivan and Ristić, Danijela",
year = "2017",
abstract = "Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.",
publisher = "Herbološko društvo Srbije",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi",
pages = "343-331",
volume = "133",
doi = "10.2298/ZMSPN1733331Z"
}

Zivkovic, S., Stošić, S., Stevanović, M., Gašić, K., Aleksic, G., Vučurović, I.,& Ristić, D.. (2017). Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke
Herbološko društvo Srbije., 133, 331-343.
https://doi.org/10.2298/ZMSPN1733331Z

Zivkovic S, Stošić S, Stevanović M, Gašić K, Aleksic G, Vučurović I, Ristić D. Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke. 2017;133:331-343.
doi:10.2298/ZMSPN1733331Z .

Zivkovic, Svetlana, Stošić, Stefan, Stevanović, Miloš, Gašić, Katarina, Aleksic, Goran, Vučurović, Ivan, Ristić, Danijela, "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi" in Zbornik Matice srpske za prirodne nauke, 133 (2017):331-343,
https://doi.org/10.2298/ZMSPN1733331Z . .

TY  - JOUR
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Aleksić, Goran
AU  - Pavlović, Snežana
AU  - Özcan, Musa, Mehmet
AU  - Knežević, Magdalena
AU  - Jošić, Dragana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/500
AB  - Development of natural plant protection products as an alternative to synthetic fungicides is of significant importance regarding the environment. This study was carried out with an objective to investigate in vitro antifungal activities of several essential oils extracted from oregano, basil, myrtle and Turkish pickling herb, and the plant growth-promoting rhizobacteria in the genus Pseudomonas, against the phytopathogenic fungus Phomopsis theicola. Microdilution methods were used to determine the minimum inhibitory concentrations (MIC) of selected antimicrobial essential oils (EOs). All EOs exhibited significant levels of antifungal activity against the tested fungal isolates. The oregano EO was found the most potent one (MIC - 5.5 µg/mL), followed by basil (MIC - 75.0µg/mL), myrtle (MIC - 775 µg/mL) and Turkish pickling herb (MIC - 7750 µg/mL). Inhibition of Ph. theicola mycelial growth was observed for all tested Pseudomonas spp. strains. K113 and L1 strains were highly effective and achieved more than 60% of fungal growth inhibition using the overnight culture and more than 57% inhibition by applying cell-free supernatants of both strains. A future field trial with K113 and L1 cultures and cell-free supernatants, containing extracellular metabolites toward Ph. theicola, will estimate their effectiveness and applicability as an alternative to chemical protection of apple trees.
AB  - U novije vreme intezivno se radi na razvoju bioloških sredstava za zaštitu bilja, koja bi se uvodila kao zamena za sintetičke fungicide. U ovom radu ispitivan je in vitro antifungalni uticaj nekih etarskih ulja (EU) i odabranih rizobakterija koje stimulišu rast biljaka iz roda Pseudomonas na fitopatogenu gljivu Phomopsis theicola. Minimalne inhibitorne koncentracije (MIC) etarskih ulja su određene mikrodilucionom metodom. Sva primenjena EU su ispoljila značajni antifungalni efekat na ispitivani izolat gljive. EU origana je ispoljilo najnižu MIC od 5.5±0.51 µg/mL, zatim ulje bosiljka od 75.0±5.7 µg/mL, mirte 775±45.0 µg/mL i turske kisele biljke od 7750±4.5 µg/mL. Proučavan je stepen inhibicije porasta micelije Ph. theicola primenom različitih sojeva Pseudomonas spp. sojevi K113 i L1 su ispoljili visoku efikasnost inhibicije od preko 60% primenom dvadesetčetvoročasovnih kultura i preko 57% primenom filtrata supernatanta. U narednim ogledima u polju primenom kulture i supernatanta K113 i L1, koji sadrže ekstracelularne metabolite, proceniće se njihova efikasnost i mogućnost korišćenja kao alternative hemijskim sredstvima u zaštiti jabuke od Ph. theicola.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola
T1  - Antifungalana aktivnost biljnih etarskih ulja i odabranih sojeva Pseudomonas spp. na Phomopsis theicola
EP  - 127
IS  - 2
SP  - 121
VL  - 32
DO  - 10.2298/PIF1702121S
ER  - 

@article{
author = "Starović, Mira and Ristić, Danijela and Aleksić, Goran and Pavlović, Snežana and Özcan, Musa, Mehmet and Knežević, Magdalena and Jošić, Dragana",
year = "2017",
abstract = "Development of natural plant protection products as an alternative to synthetic fungicides is of significant importance regarding the environment. This study was carried out with an objective to investigate in vitro antifungal activities of several essential oils extracted from oregano, basil, myrtle and Turkish pickling herb, and the plant growth-promoting rhizobacteria in the genus Pseudomonas, against the phytopathogenic fungus Phomopsis theicola. Microdilution methods were used to determine the minimum inhibitory concentrations (MIC) of selected antimicrobial essential oils (EOs). All EOs exhibited significant levels of antifungal activity against the tested fungal isolates. The oregano EO was found the most potent one (MIC - 5.5 µg/mL), followed by basil (MIC - 75.0µg/mL), myrtle (MIC - 775 µg/mL) and Turkish pickling herb (MIC - 7750 µg/mL). Inhibition of Ph. theicola mycelial growth was observed for all tested Pseudomonas spp. strains. K113 and L1 strains were highly effective and achieved more than 60% of fungal growth inhibition using the overnight culture and more than 57% inhibition by applying cell-free supernatants of both strains. A future field trial with K113 and L1 cultures and cell-free supernatants, containing extracellular metabolites toward Ph. theicola, will estimate their effectiveness and applicability as an alternative to chemical protection of apple trees., U novije vreme intezivno se radi na razvoju bioloških sredstava za zaštitu bilja, koja bi se uvodila kao zamena za sintetičke fungicide. U ovom radu ispitivan je in vitro antifungalni uticaj nekih etarskih ulja (EU) i odabranih rizobakterija koje stimulišu rast biljaka iz roda Pseudomonas na fitopatogenu gljivu Phomopsis theicola. Minimalne inhibitorne koncentracije (MIC) etarskih ulja su određene mikrodilucionom metodom. Sva primenjena EU su ispoljila značajni antifungalni efekat na ispitivani izolat gljive. EU origana je ispoljilo najnižu MIC od 5.5±0.51 µg/mL, zatim ulje bosiljka od 75.0±5.7 µg/mL, mirte 775±45.0 µg/mL i turske kisele biljke od 7750±4.5 µg/mL. Proučavan je stepen inhibicije porasta micelije Ph. theicola primenom različitih sojeva Pseudomonas spp. sojevi K113 i L1 su ispoljili visoku efikasnost inhibicije od preko 60% primenom dvadesetčetvoročasovnih kultura i preko 57% primenom filtrata supernatanta. U narednim ogledima u polju primenom kulture i supernatanta K113 i L1, koji sadrže ekstracelularne metabolite, proceniće se njihova efikasnost i mogućnost korišćenja kao alternative hemijskim sredstvima u zaštiti jabuke od Ph. theicola.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola, Antifungalana aktivnost biljnih etarskih ulja i odabranih sojeva Pseudomonas spp. na Phomopsis theicola",
pages = "127-121",
number = "2",
volume = "32",
doi = "10.2298/PIF1702121S"
}

Starović, M., Ristić, D., Aleksić, G., Pavlović, S., Özcan, M. M., Knežević, M.,& Jošić, D.. (2017). Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(2), 121-127.
https://doi.org/10.2298/PIF1702121S

Starović M, Ristić D, Aleksić G, Pavlović S, Özcan MM, Knežević M, Jošić D. Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola. in Pesticidi i fitomedicina. 2017;32(2):121-127.
doi:10.2298/PIF1702121S .

Starović, Mira, Ristić, Danijela, Aleksić, Goran, Pavlović, Snežana, Özcan, Musa, Mehmet, Knežević, Magdalena, Jošić, Dragana, "Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola" in Pesticidi i fitomedicina, 32, no. 2 (2017):121-127,
https://doi.org/10.2298/PIF1702121S . .

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Nikolić, Dušan
AU  - Radović, Nikola
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/492
AB  - Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
AB  - Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Incidence and distribution of leek yellow stripe virus in allium crops in Serbia
EP  - 155
IS  - 3-4
SP  - 145
VL  - 32
DO  - 10.2298/PIF1704145V
ER  - 

@article{
author = "Vučurović, Ivan and Nikolić, Dušan and Radović, Nikola and Vučurović, Ana and Ristić, Danijela and Krstić, Branka and Stanković, Ivana",
year = "2017",
abstract = "Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E., Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia",
pages = "155-145",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704145V"
}

Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B.,& Stanković, I.. (2017). Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 145-155.
https://doi.org/10.2298/PIF1704145V

Vučurović I, Nikolić D, Radović N, Vučurović A, Ristić D, Krstić B, Stanković I. Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):145-155.
doi:10.2298/PIF1704145V .

Vučurović, Ivan, Nikolić, Dušan, Radović, Nikola, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, Stanković, Ivana, "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):145-155,
https://doi.org/10.2298/PIF1704145V . .

TY  - JOUR
AU  - Kuzmanović, Slobodan
AU  - Aleksic, Goran
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1046
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Fitoplazmoze vinove loze – mere borbe
EP  - 54
IS  - 5
SP  - 47
VL  - 22
ER  - 

@article{
author = "Kuzmanović, Slobodan and Aleksic, Goran and Ristić, Danijela and Vučurović, Ivan and Starović, Mira",
year = "2016",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Fitoplazmoze vinove loze – mere borbe",
pages = "54-47",
number = "5",
volume = "22"
}

Kuzmanović, S., Aleksic, G., Ristić, D., Vučurović, I.,& Starović, M.. (2016). Fitoplazmoze vinove loze – mere borbe. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 22(5), 47-54.

Kuzmanović S, Aleksic G, Ristić D, Vučurović I, Starović M. Fitoplazmoze vinove loze – mere borbe. in Zbornik Instituta PKB agroekonomik. 2016;22(5):47-54..

Kuzmanović, Slobodan, Aleksic, Goran, Ristić, Danijela, Vučurović, Ivan, Starović, Mira, "Fitoplazmoze vinove loze – mere borbe" in Zbornik Instituta PKB agroekonomik, 22, no. 5 (2016):47-54.

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
AU  - Stojanović, Sasa
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/450
AB  - Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.
PB  - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia
EP  - 417
IS  - 2
SP  - 411
VL  - 44
DO  - 10.15835/nbha44210488
ER  - 

@article{
author = "Pavlović, Snežana and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš and Stojanović, Sasa and Kuzmanović, Slobodan and Starović, Mira",
year = "2016",
abstract = "Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.",
publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia",
pages = "417-411",
number = "2",
volume = "44",
doi = "10.15835/nbha44210488"
}

Pavlović, S., Ristić, D., Vučurović, I., Stevanović, M., Stojanović, S., Kuzmanović, S.,& Starović, M.. (2016). Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 44(2), 411-417.
https://doi.org/10.15835/nbha44210488

Pavlović S, Ristić D, Vučurović I, Stevanović M, Stojanović S, Kuzmanović S, Starović M. Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2016;44(2):411-417.
doi:10.15835/nbha44210488 .

Pavlović, Snežana, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, Stojanović, Sasa, Kuzmanović, Slobodan, Starović, Mira, "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44, no. 2 (2016):411-417,
https://doi.org/10.15835/nbha44210488 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Milosević, Drago
AU  - Gašić, Katarina
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/449
AB  - The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia
EP  - 496
IS  - 2
SP  - 487
VL  - 48
DO  - 10.2298/GENSR1602487R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Milosević, Drago and Gašić, Katarina and Dolovac, Nenad and Starović, Mira",
year = "2016",
abstract = "The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia",
pages = "496-487",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602487R"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Milosević, D., Gašić, K., Dolovac, N.,& Starović, M.. (2016). Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 48(2), 487-496.
https://doi.org/10.2298/GENSR1602487R

Ristić D, Vučurović I, Kuzmanović S, Milosević D, Gašić K, Dolovac N, Starović M. Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade. 2016;48(2):487-496.
doi:10.2298/GENSR1602487R .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Milosević, Drago, Gašić, Katarina, Dolovac, Nenad, Starović, Mira, "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia" in Genetika-Belgrade, 48, no. 2 (2016):487-496,
https://doi.org/10.2298/GENSR1602487R . .

TY  - JOUR
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - Ristić, Mihailo
AU  - Stevanović, Miloš
AU  - AlJuhaimi, Fahad
AU  - Naydun, Svetlana
AU  - Ozcan, Mehmet Musa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/441
AB  - The aim of this study was to investigate the possibility of biological control of fungal species isolated from anise seeds using essential oils from medicinal plants: mint (Mentha spicata L.), sage (Salvia fruticosa L.), rosemary (Rosmarinus officinalis L.), anise (Pimpinella anisum L.), bitter fennel (Foeniculum vulgare spp. piperituum L.) and myrtle (Myrtus communis L.). Ten fungal species isolated from anise seeds: Bipolaris/Drechslera sorociniana, Fusarium subglutinans, F. vertricilioides, F. oxysporum, F. tricinctum, F sporotrichioides, F. equiseti, F. incarnatum, F proliferatum and Macrophomina phaseolina, were used in this experiment The minimum inhibitory concentrations (MIC) were determined by micro-dilution method using selected essential oils (EOs). A qualitative and quantitative chemical analyses of EOs were carried out. All EOs exhibited a significant antifungal activity against all tested fungal isolates. The myrtle EO proved to be the most potent one (MIC 0.0003-3.25 mg/mL, then mint 0.0003-7.75 mg/mL and sage 0.0003-10 mg/mL). All tested fungi were observed to have a susceptibility to all selected essential oils. These results suggest the possibility for application of the EOs in biological control of anise production.
PB  - M H Schaper Gmbh Co Kg, Alfeld
T2  - Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene
T1  - Antifungal activities of different essential oils against anise seeds mycopopulations
EP  - 78
IS  - 3
SP  - 72
VL  - 67
DO  - 10.2376/0003-925X-67-72
ER  - 

@article{
author = "Starović, Mira and Ristić, Danijela and Pavlović, Snežana and Ristić, Mihailo and Stevanović, Miloš and AlJuhaimi, Fahad and Naydun, Svetlana and Ozcan, Mehmet Musa",
year = "2016",
abstract = "The aim of this study was to investigate the possibility of biological control of fungal species isolated from anise seeds using essential oils from medicinal plants: mint (Mentha spicata L.), sage (Salvia fruticosa L.), rosemary (Rosmarinus officinalis L.), anise (Pimpinella anisum L.), bitter fennel (Foeniculum vulgare spp. piperituum L.) and myrtle (Myrtus communis L.). Ten fungal species isolated from anise seeds: Bipolaris/Drechslera sorociniana, Fusarium subglutinans, F. vertricilioides, F. oxysporum, F. tricinctum, F sporotrichioides, F. equiseti, F. incarnatum, F proliferatum and Macrophomina phaseolina, were used in this experiment The minimum inhibitory concentrations (MIC) were determined by micro-dilution method using selected essential oils (EOs). A qualitative and quantitative chemical analyses of EOs were carried out. All EOs exhibited a significant antifungal activity against all tested fungal isolates. The myrtle EO proved to be the most potent one (MIC 0.0003-3.25 mg/mL, then mint 0.0003-7.75 mg/mL and sage 0.0003-10 mg/mL). All tested fungi were observed to have a susceptibility to all selected essential oils. These results suggest the possibility for application of the EOs in biological control of anise production.",
publisher = "M H Schaper Gmbh Co Kg, Alfeld",
journal = "Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene",
title = "Antifungal activities of different essential oils against anise seeds mycopopulations",
pages = "78-72",
number = "3",
volume = "67",
doi = "10.2376/0003-925X-67-72"
}

Starović, M., Ristić, D., Pavlović, S., Ristić, M., Stevanović, M., AlJuhaimi, F., Naydun, S.,& Ozcan, M. M.. (2016). Antifungal activities of different essential oils against anise seeds mycopopulations. in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene
M H Schaper Gmbh Co Kg, Alfeld., 67(3), 72-78.
https://doi.org/10.2376/0003-925X-67-72

Starović M, Ristić D, Pavlović S, Ristić M, Stevanović M, AlJuhaimi F, Naydun S, Ozcan MM. Antifungal activities of different essential oils against anise seeds mycopopulations. in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene. 2016;67(3):72-78.
doi:10.2376/0003-925X-67-72 .

Starović, Mira, Ristić, Danijela, Pavlović, Snežana, Ristić, Mihailo, Stevanović, Miloš, AlJuhaimi, Fahad, Naydun, Svetlana, Ozcan, Mehmet Musa, "Antifungal activities of different essential oils against anise seeds mycopopulations" in Journal of Food Safety and Food Quality - Archiv fur Lebensmittelhygiene, 67, no. 3 (2016):72-78,
https://doi.org/10.2376/0003-925X-67-72 . .