TY  - JOUR
AU  - Malembic-Maher, Sylvie
AU  - Desque, Delphine
AU  - Khalil, Dima
AU  - Salar, Pascal
AU  - Bergey, Bernard
AU  - Danet, Jean-Luc
AU  - Duret, Sybille
AU  - Dubrana-Ourabah, Marie-Pierre
AU  - Beven, Laure
AU  - Ember, Ibolyia
AU  - Acs, Zoltan
AU  - Della Bartola, Michele
AU  - MATERAZZI, ALBERTO
AU  - Fillipin, Luisa
AU  - Krnjajić, Slobodan
AU  - Krstić, Oliver
AU  - Tosevski, Ivo
AU  - Lang, Friederike
AU  - Jarausch, Barbara
AU  - Kolber, Maria
AU  - Jovic, Jelena
AU  - angelini, elisa
AU  - Arricau-Bouvery, Nathalie
AU  - Maixner, Michael
AU  - Foissac, Xavier
PY  - 2020-03-25
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/644
AB  - Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and–III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties.
PB  - Public Library of Science
T2  - PLOS PATHOGENS
T1  - When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe
IS  - 3
VL  - 16
DO  - 10.1371/journal.ppat.1007967
ER  - 

@article{
author = "Malembic-Maher, Sylvie and Desque, Delphine and Khalil, Dima and Salar, Pascal and Bergey, Bernard and Danet, Jean-Luc and Duret, Sybille and Dubrana-Ourabah, Marie-Pierre and Beven, Laure and Ember, Ibolyia and Acs, Zoltan and Della Bartola, Michele and MATERAZZI, ALBERTO and Fillipin, Luisa and Krnjajić, Slobodan and Krstić, Oliver and Tosevski, Ivo and Lang, Friederike and Jarausch, Barbara and Kolber, Maria and Jovic, Jelena and angelini, elisa and Arricau-Bouvery, Nathalie and Maixner, Michael and Foissac, Xavier",
year = "2020-03-25",
abstract = "Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and–III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties.",
publisher = "Public Library of Science",
journal = "PLOS PATHOGENS",
title = "When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe",
number = "3",
volume = "16",
doi = "10.1371/journal.ppat.1007967"
}

Malembic-Maher, S., Desque, D., Khalil, D., Salar, P., Bergey, B., Danet, J., Duret, S., Dubrana-Ourabah, M., Beven, L., Ember, I., Acs, Z., Della Bartola, M., MATERAZZI, A., Fillipin, L., Krnjajić, S., Krstić, O., Tosevski, I., Lang, F., Jarausch, B., Kolber, M., Jovic, J., angelini, e., Arricau-Bouvery, N., Maixner, M.,& Foissac, X.. (2020-03-25). When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe. in PLOS PATHOGENS
Public Library of Science., 16(3).
https://doi.org/10.1371/journal.ppat.1007967

Malembic-Maher S, Desque D, Khalil D, Salar P, Bergey B, Danet J, Duret S, Dubrana-Ourabah M, Beven L, Ember I, Acs Z, Della Bartola M, MATERAZZI A, Fillipin L, Krnjajić S, Krstić O, Tosevski I, Lang F, Jarausch B, Kolber M, Jovic J, angelini E, Arricau-Bouvery N, Maixner M, Foissac X. When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe. in PLOS PATHOGENS. 2020;16(3).
doi:10.1371/journal.ppat.1007967 .

Malembic-Maher, Sylvie, Desque, Delphine, Khalil, Dima, Salar, Pascal, Bergey, Bernard, Danet, Jean-Luc, Duret, Sybille, Dubrana-Ourabah, Marie-Pierre, Beven, Laure, Ember, Ibolyia, Acs, Zoltan, Della Bartola, Michele, MATERAZZI, ALBERTO, Fillipin, Luisa, Krnjajić, Slobodan, Krstić, Oliver, Tosevski, Ivo, Lang, Friederike, Jarausch, Barbara, Kolber, Maria, Jovic, Jelena, angelini, elisa, Arricau-Bouvery, Nathalie, Maixner, Michael, Foissac, Xavier, "When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe" in PLOS PATHOGENS, 16, no. 3 (2020-03-25),
https://doi.org/10.1371/journal.ppat.1007967 . .

TY  - CONF
AU  - Malembic-Maher, Sylvie
AU  - Desque, Delphine
AU  - Khalil, Dima
AU  - Salar, Pascal
AU  - Danet, Jean Luc
AU  - Dubrana-Ourabah, Marie-Pierre
AU  - Duret, Sybille
AU  - Ember, Ibolya
AU  - Acs, Zoltan
AU  - Bartola, Michele Della
AU  - Materazzi, Alberto
AU  - Filippin, Luisa
AU  - Krnjajic, Slobodan
AU  - Tosevski, Ivo
AU  - Lang, Friederik
AU  - Jarausch, Barbara
AU  - Kolber, Maria
AU  - Jovic, Jelena
AU  - Angelini, Elisa
AU  - Arricau-Bouvery, Nathalie
AU  - Maixner, Michael
AU  - Foissac, Xavier
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1050
AB  - A survey of genetic diversity of “flavescence dorée” (FD)-related phytoplasmas in grapevines, alders and clematis as well as alder-feeding leafhoppers was conducted in France, Hungary, Germany, Italy and Serbia. Genotyping was based on the housekeeping gene map and on the vmp genes encoding surface variable membrane proteins. Transmission assays of the phytoplasmas were performed with alder and/or grapevine-feeding leafhoppers. The study demonstrated that European alders constitute an original reservoir of FD phytoplasma by hosting a high diversity of FD-related phytoplasma genotypes, also present in non viticultural areas. The alder phytoplasmas grouping in Vmp-I cluster were transmitted by the Macropsinae O. alni, but were not compatible with the FD phytoplasma vector on grapevine the Deltocephalinae Scaphoideus titanus. The alder phytoplasmas in Vmp-II and-III clusters were transmitted by the Deltocephalinae Allygus spp. and Orientus ishidae. Such pre-existing phytoplasmas were compatible with S. titanus transmissibility and can be responsible for the emergence of FD phytoplasma epidemics in grapevine. VmpA proteins of cluster II better adhered to Euscelidius variegatus and S. titanus insect cells and midguts than those of cluster I. Such adhesins might play a key role in the adaptation to new vectors.
PB  - Indian journals Diva Enterprises PVT
C3  - Phytopathogenic Mollicutes
T1  - New insights into the emergence of the grapevine "flavescence dorée" epidemics in Europe
EP  - 206
IS  - 1
SP  - 205
VL  - 9
DO  - 10.5958/2249-4677.2019.00103.8
ER  - 

@conference{
author = "Malembic-Maher, Sylvie and Desque, Delphine and Khalil, Dima and Salar, Pascal and Danet, Jean Luc and Dubrana-Ourabah, Marie-Pierre and Duret, Sybille and Ember, Ibolya and Acs, Zoltan and Bartola, Michele Della and Materazzi, Alberto and Filippin, Luisa and Krnjajic, Slobodan and Tosevski, Ivo and Lang, Friederik and Jarausch, Barbara and Kolber, Maria and Jovic, Jelena and Angelini, Elisa and Arricau-Bouvery, Nathalie and Maixner, Michael and Foissac, Xavier",
year = "2019",
abstract = "A survey of genetic diversity of “flavescence dorée” (FD)-related phytoplasmas in grapevines, alders and clematis as well as alder-feeding leafhoppers was conducted in France, Hungary, Germany, Italy and Serbia. Genotyping was based on the housekeeping gene map and on the vmp genes encoding surface variable membrane proteins. Transmission assays of the phytoplasmas were performed with alder and/or grapevine-feeding leafhoppers. The study demonstrated that European alders constitute an original reservoir of FD phytoplasma by hosting a high diversity of FD-related phytoplasma genotypes, also present in non viticultural areas. The alder phytoplasmas grouping in Vmp-I cluster were transmitted by the Macropsinae O. alni, but were not compatible with the FD phytoplasma vector on grapevine the Deltocephalinae Scaphoideus titanus. The alder phytoplasmas in Vmp-II and-III clusters were transmitted by the Deltocephalinae Allygus spp. and Orientus ishidae. Such pre-existing phytoplasmas were compatible with S. titanus transmissibility and can be responsible for the emergence of FD phytoplasma epidemics in grapevine. VmpA proteins of cluster II better adhered to Euscelidius variegatus and S. titanus insect cells and midguts than those of cluster I. Such adhesins might play a key role in the adaptation to new vectors.",
publisher = "Indian journals Diva Enterprises PVT",
journal = "Phytopathogenic Mollicutes",
title = "New insights into the emergence of the grapevine "flavescence dorée" epidemics in Europe",
pages = "206-205",
number = "1",
volume = "9",
doi = "10.5958/2249-4677.2019.00103.8"
}

Malembic-Maher, S., Desque, D., Khalil, D., Salar, P., Danet, J. L., Dubrana-Ourabah, M., Duret, S., Ember, I., Acs, Z., Bartola, M. D., Materazzi, A., Filippin, L., Krnjajic, S., Tosevski, I., Lang, F., Jarausch, B., Kolber, M., Jovic, J., Angelini, E., Arricau-Bouvery, N., Maixner, M.,& Foissac, X.. (2019). New insights into the emergence of the grapevine "flavescence dorée" epidemics in Europe. in Phytopathogenic Mollicutes
Indian journals Diva Enterprises PVT., 9(1), 205-206.
https://doi.org/10.5958/2249-4677.2019.00103.8

Malembic-Maher S, Desque D, Khalil D, Salar P, Danet JL, Dubrana-Ourabah M, Duret S, Ember I, Acs Z, Bartola MD, Materazzi A, Filippin L, Krnjajic S, Tosevski I, Lang F, Jarausch B, Kolber M, Jovic J, Angelini E, Arricau-Bouvery N, Maixner M, Foissac X. New insights into the emergence of the grapevine "flavescence dorée" epidemics in Europe. in Phytopathogenic Mollicutes. 2019;9(1):205-206.
doi:10.5958/2249-4677.2019.00103.8 .

Malembic-Maher, Sylvie, Desque, Delphine, Khalil, Dima, Salar, Pascal, Danet, Jean Luc, Dubrana-Ourabah, Marie-Pierre, Duret, Sybille, Ember, Ibolya, Acs, Zoltan, Bartola, Michele Della, Materazzi, Alberto, Filippin, Luisa, Krnjajic, Slobodan, Tosevski, Ivo, Lang, Friederik, Jarausch, Barbara, Kolber, Maria, Jovic, Jelena, Angelini, Elisa, Arricau-Bouvery, Nathalie, Maixner, Michael, Foissac, Xavier, "New insights into the emergence of the grapevine "flavescence dorée" epidemics in Europe" in Phytopathogenic Mollicutes, 9, no. 1 (2019):205-206,
https://doi.org/10.5958/2249-4677.2019.00103.8 . .