Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi
Аутори
Zivkovic, SvetlanaStošić, Stefan
Stevanović, Miloš
Gašić, Katarina
Aleksic, Goran
Vučurović, Ivan
Ristić, Danijela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequenc...es were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.
Кључне речи:
Colletotrichum orbiculare / watermelon / identification / antagonistic fungiИзвор:
Zbornik Matice srpske za prirodne nauke, 2017, 133, 331-343Издавач:
- Herbološko društvo Srbije
Финансирање / пројекти:
- Разрада интегрисаног управљања и примене савремених принципа сузбијања штетних организама у заштити биља (RS-MESTD-Technological Development (TD or TR)-31018)
- Молекуларна карактеризација бактерија из родова Bacillus и Pseudomonas као потенцијалних агенаса за биолошку контролу (RS-MESTD-Basic Research (BR or ON)-173026)
Институција/група
IZBISTY - JOUR AU - Zivkovic, Svetlana AU - Stošić, Stefan AU - Stevanović, Miloš AU - Gašić, Katarina AU - Aleksic, Goran AU - Vučurović, Ivan AU - Ristić, Danijela PY - 2017 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1013 AB - Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits. PB - Herbološko društvo Srbije T2 - Zbornik Matice srpske za prirodne nauke T1 - Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi EP - 343 SP - 331 VL - 133 DO - 10.2298/ZMSPN1733331Z ER -
@article{ author = "Zivkovic, Svetlana and Stošić, Stefan and Stevanović, Miloš and Gašić, Katarina and Aleksic, Goran and Vučurović, Ivan and Ristić, Danijela", year = "2017", abstract = "Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.", publisher = "Herbološko društvo Srbije", journal = "Zbornik Matice srpske za prirodne nauke", title = "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi", pages = "343-331", volume = "133", doi = "10.2298/ZMSPN1733331Z" }
Zivkovic, S., Stošić, S., Stevanović, M., Gašić, K., Aleksic, G., Vučurović, I.,& Ristić, D.. (2017). Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke Herbološko društvo Srbije., 133, 331-343. https://doi.org/10.2298/ZMSPN1733331Z
Zivkovic S, Stošić S, Stevanović M, Gašić K, Aleksic G, Vučurović I, Ristić D. Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke. 2017;133:331-343. doi:10.2298/ZMSPN1733331Z .
Zivkovic, Svetlana, Stošić, Stefan, Stevanović, Miloš, Gašić, Katarina, Aleksic, Goran, Vučurović, Ivan, Ristić, Danijela, "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi" in Zbornik Matice srpske za prirodne nauke, 133 (2017):331-343, https://doi.org/10.2298/ZMSPN1733331Z . .