Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia

Abstract

Pseudomonas syringae is a widespread and economically important plant pathogen, one found on a number of hosts, including fruit trees, field crops, vegetables, and ornamental plants. This bacterium has been experimentally identified as a parasite of pear, apple, apricot, peach, cherry, sour cherry, plum, and raspberry. The present study was designed to establish differences between strains isolated from fruit trees in Serbia. The pathogenic and biochemical characteristics of isolates were studied. The BOX-PCR method was used to generate genomic fingerprints of Pseudomonas syringae isolates and to identify strains that were previously not distinguishable by other classification methods. Different Bacillus sp. strains were tested for in vitro inhibitory activity against Pseudononas syringae isolates. Bacillus sp. strains show inhibitory activity only against P. syringae isolates that originated from peach. The obtained results demonstrate that the population of the bacterium Pseudomonas syringae from the fruit trees in Serbia is very diverse.

Pseudomonas syringae je široko rasprostranjena i ekonomski značajna fitopatogena bakterija, sa širokim krugom domaćina koji uključuje voćke, ratarske, povrtarske i ukrasne biljke. Pseudomonas syringae u Srbiji je eksperimentalno potvrđen kao parazit kruške, jabuke, kajsije, breskve, trešnje, višnje, šljive i maline. Cilj ove studije bio je da utvrdi postojanje eventualnih razlika između sojeva izolovanih sa različitih vrsta voćaka u Srbiji. Proučavane su patogene i biohemijske osobine sojeva. BOX-PCR je korišćen za dobijanje profila izolata Pseudomonas syringae u cilju identifikacije sojeva koji se ne mogu utvrditi drugim metodama. Različiti sojevi roda Bacillus su testirani u cilju utvrđivanja njihove in vitro inhibitorne aktivnosti. Sojevi roda Bacillus su pokazali inhibitornu aktivnost samo na P. syringae izolovanih sa breskve. Dobijeni rezultati pokazali suda je populacija bakterije Pseudomonas syringae poreklom sa voća u Srbiji vrlo raznovrsna.