Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates
Само за регистроване кориснике
2012
Аутори
Vučurović, AnaBulajić, Aleksandra
Stanković, Ivana
Ristić, Danijela
Berenji, Janoš
Jović, Jelena
Krstić, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequ...ences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.
Кључне речи:
Cucurbit viruses / RT-PCR / Sequencing / Serological identification / Virus incidenceИзвор:
European Journal of Plant Pathology, 2012, 133, 4, 935-947Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Агробиодиверзитет и коришћење земљишта у Србији: интегрисана процена биодиверзитета кључних група артропода и биљних патогена (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
- Развој нових сорти и побољшање технологија производње уљаних биљних врста за различите намене (RS-MESTD-Technological Development (TD or TR)-31025)
DOI: 10.1007/s10658-012-9964-x
ISSN: 0929-1873
WoS: 000305687000017
Scopus: 2-s2.0-84862844938
Институција/група
IZBISTY - JOUR AU - Vučurović, Ana AU - Bulajić, Aleksandra AU - Stanković, Ivana AU - Ristić, Danijela AU - Berenji, Janoš AU - Jović, Jelena AU - Krstić, Branka PY - 2012 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/212 AB - Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates EP - 947 IS - 4 SP - 935 VL - 133 DO - 10.1007/s10658-012-9964-x ER -
@article{ author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka", year = "2012", abstract = "Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates", pages = "947-935", number = "4", volume = "133", doi = "10.1007/s10658-012-9964-x" }
Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2012). Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology Springer, Dordrecht., 133(4), 935-947. https://doi.org/10.1007/s10658-012-9964-x
Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology. 2012;133(4):935-947. doi:10.1007/s10658-012-9964-x .
Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates" in European Journal of Plant Pathology, 133, no. 4 (2012):935-947, https://doi.org/10.1007/s10658-012-9964-x . .