Razvoj mikoparazita stroma Polystigma rubrum subsp. rubrum in-vitro

Abstract

The effects of temperature, sun- and artificial light, pH-value and different media on development of Colletotrichum gloeosporioides, isolated from stromata of Polystigma rubrum subsp. rubrum, were studied in-vitro. Colonies of mycoparasite grew in wide range of temperature at the minimum beneath 5ºC and maximum at 30-35ºC. Abundant sporulation was at 20-30ºC. Sun light, as well as fluorescent and near-UV light, decreased colony growth, but stimulated sporulation. The best colony growth and more abundant sporulation were obtained at pH 6-7, although there was abundant sporulation on all medium pH tested. The most media were sutible for mycoparasite development, except corn meal agar (CMA) and potato carrot agar (PCA).

Uticaj temperature, sunčeve i veštačke svetlosti, pH podloge i raznih podloga na razvoj mikoparazita (Colletotrichum gloeosporioides) izolovanog sa stroma Polystigma rubrum subsp. rubrum ispitivan je in-vitro. Optimalna temperatura za porast kolonije mikoparazita je 20-25º, minimalna ispod 5ºC i maksimalna 30-35ºC. Obilna sporulacija gljive je na temperaturama između 20-30ºC. Sunčeva, fluoroscentna i UV svetlost utiču na smanjenje porasta kolonija, ali stimulišu fruktifikaciju gljive. Najbolji porast kolonija i najobilnija fruktifikacija dobivena je pri pH podloge 6-7, mada je obilna sporulacija ostvarena na svim testiranim pH vrednostima podloge. Ispitivane podloge, osim podloge od kukuruznog brašna i podloge od krompira i mrkve, bile su pogodne za razvoj mikoparazita.