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dc.creatorTrkulja, Nenad
dc.creatorIvanović, Žarko
dc.creatorPfaf-Dolovac, Erika
dc.creatorDolovac, Nenad
dc.creatorMitrović, Milana
dc.creatorToševski, Ivo
dc.creatorJović, Jelena
dc.date.accessioned2019-04-04T15:58:37Z
dc.date.available2019-04-04T15:58:37Z
dc.date.issued2013
dc.identifier.issn0929-1873
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/272
dc.description.abstractA survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31018/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Plant Pathology
dc.subjectCercospora leaf spoten
dc.subjectNovel point mutation Phe167Tyren
dc.subjectPCR-RFLP diagnosticsen
dc.subjectPCR mutation-specific diagnostic assayen
dc.subjectSugar beeten
dc.subjectTemperature sensitivityen
dc.titleCharacterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin geneen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage902
dc.citation.issue4
dc.citation.other135(4): 889-902
dc.citation.rankM21
dc.citation.spage889
dc.citation.volume135
dc.type.versionpublishedVersion
dc.identifier.doi10.1007/s10658-012-0135-x
dc.identifier.rcubconv_842
dc.identifier.scopus2-s2.0-84874569380
dc.identifier.wos000315601500025


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