Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
Samo za registrovane korisnike
2013
Autori
Kuzmanović, NemanjaIvanović, Milan M
Prokić, Anđelka
Gašić, Katarina
Blagojević, Nevena
Pulawska, Joanna
Obradović, Aleksa
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, f...ive strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
Ključne reči:
Crown gall / Apricot / Tumorigenic / Pathogenic plasmid / OpinesIzvor:
European Journal of Plant Pathology, 2013, 137, 1, 11-16Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Ministry of Education, Science and Technological Development, Republic of Serbia
- COST Action - 873
- Polish Ministry of Science and Higher Education - 118/N-COST/2008/0
- Razvoj integrisanih sistema upravljanja štetnim organizmima u biljnoj proizvodnji sa ciljem prevazilaženja rezistentnosti i unapređenja kvaliteta i bezbednosti hrane (RS-46008)
DOI: 10.1007/s10658-013-0229-0
ISSN: 0929-1873
WoS: 000322880200003
Scopus: 2-s2.0-84881230679
Institucija/grupa
IZBISTY - JOUR AU - Kuzmanović, Nemanja AU - Ivanović, Milan M AU - Prokić, Anđelka AU - Gašić, Katarina AU - Blagojević, Nevena AU - Pulawska, Joanna AU - Obradović, Aleksa PY - 2013 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/299 AB - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia EP - 16 IS - 1 SP - 11 VL - 137 DO - 10.1007/s10658-013-0229-0 ER -
@article{ author = "Kuzmanović, Nemanja and Ivanović, Milan M and Prokić, Anđelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa", year = "2013", abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia", pages = "16-11", number = "1", volume = "137", doi = "10.1007/s10658-013-0229-0" }
Kuzmanović, N., Ivanović, M. M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology Springer, Dordrecht., 137(1), 11-16. https://doi.org/10.1007/s10658-013-0229-0
Kuzmanović N, Ivanović MM, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16. doi:10.1007/s10658-013-0229-0 .
Kuzmanović, Nemanja, Ivanović, Milan M, Prokić, Anđelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16, https://doi.org/10.1007/s10658-013-0229-0 . .