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dc.creatorKuzmanović, Nemanja
dc.creatorIvanović, Milan
dc.creatorProkić, Anđelka
dc.creatorGašić, Katarina
dc.creatorZlatković, Nevena
dc.creatorObradović, Aleksa
dc.date.accessioned2019-04-04T15:59:43Z
dc.date.available2019-04-04T15:59:43Z
dc.date.issued2014
dc.identifier.issn0929-1873
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/307
dc.description.abstractSerious outbreaks of grapevine crown gall disease were observed in major Serbian viticultural regions during the last five years. Tumorigenic Agrobacterium vitis was identified as a causal agent by using conventional bacteriological and molecular tests. The 36 studied strains of A. vitis showed homogeneous biochemical and physiological characteristics, but were heterogeneous in their pathogenic properties, especially on tomato and sunflower. Furthermore, genetic differences related to chromosomal and plasmid DNA were observed. The Ti plasmid of 35 strains was classified as the octopine/cucumopine (O/C) type, whereas one was classified as the vitopine (V) type. The O/C strains were further divided into O/C-1 and O/C-2 groups based on PCR analysis. Moreover, the sequence analysis of the 16S-23S rRNA ITS region provided robust and precise delineation of studied strains. Although a high level of genetic diversity in A. vitis strains from Serbia was revealed by using this approach, their genotypic relatedness with the strains from other countries suggested their common origin. Also, association between the chromosomal and plasmid DNA was determined for some phylogenetic groups and clusters.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46008/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316004/EU//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Plant Pathology
dc.subjectCrown gallen
dc.subjectGrapevineen
dc.subject16S-23SrRNAITS regionen
dc.subjectTi plasmiden
dc.subjectOpine typeen
dc.titleCharacterization and phylogenetic diversity of Agrobacterium vitis from Serbia based on sequence analysis of 16S-23S rRNA internal transcribed spacer (ITS) regionen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage768
dc.citation.issue4
dc.citation.other140(4): 757-768
dc.citation.rankM21
dc.citation.spage757
dc.citation.volume140
dc.type.versionpublishedVersion
dc.identifier.doi10.1007/s10658-014-0507-5
dc.identifier.rcubconv_777
dc.identifier.scopus2-s2.0-84939874124
dc.identifier.wos000344533700013


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