The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia
Samo za registrovane korisnike
2015
Autori
Atanasova, BiljanaJakovljević, Miljana
Spasov, Dušan
Jović, Jelena
Mitrović, Milana
Toševski, Ivo
Cvrković, Tatjana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and.../or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.
Ključne reči:
Grapevine yellows / Molecular epidemiology / Hyalesthes obsoletus / Stamp variability / StolburIzvor:
European Journal of Plant Pathology, 2015, 142, 4, 759-770Izdavač:
- Springer, Dordrecht
Finansiranje / projekti:
- Agrobiodiverzitet i korišćenje zemljišta u Srbiji: integrisana procena biodiverziteta ključnih grupa artropoda i biljnih patogena (RS-43001)
- SCOPES program of the Swiss National Science Foundation - IZ73Z0_152414
DOI: 10.1007/s10658-015-0649-0
ISSN: 0929-1873
WoS: 000356731500009
Scopus: 2-s2.0-84931571517
Institucija/grupa
IZBISTY - JOUR AU - Atanasova, Biljana AU - Jakovljević, Miljana AU - Spasov, Dušan AU - Jović, Jelena AU - Mitrović, Milana AU - Toševski, Ivo AU - Cvrković, Tatjana PY - 2015 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/383 AB - Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia EP - 770 IS - 4 SP - 759 VL - 142 DO - 10.1007/s10658-015-0649-0 ER -
@article{ author = "Atanasova, Biljana and Jakovljević, Miljana and Spasov, Dušan and Jović, Jelena and Mitrović, Milana and Toševski, Ivo and Cvrković, Tatjana", year = "2015", abstract = "Bois noir (BN), which is induced by 'Candidatus Phytoplasma solani' ('Ca. P. solani'), is an important grapevine yellows disease that causes severe damage in viticultural regions throughout the Euro-Mediterranean basin. An epidemiological survey to determine potential insect vectors and the primary reservoir plants of BN phytoplasma in Macedonian vineyards was undertaken between 2012 and 2013 in the southeastern part of the country. A study on the species diversity from the suborder Auchenorrhycha revealed the prevalence of the principal vector of 'Ca. P. solani', which is the planthopper Hyalesthes obsoletus. Reptalus panzeri, which is the second-most documented BN vector, was not recorded in Macedonian vineyards. Three leafhopper species, namely Psammotettix alienus, Artianus manderstjernii and Euscelis incisus, were also widespread in the BN-affected vineyards, but only H. obsoletus tested positive for 'Ca. P. solani'. Molecular characterizations were performed by the sequencing and/or RFLP typing of tuf, vmp1 and stamp genes, and the results were used to gain detailed insight into the molecular diversity of the 'Ca. P. solani' strains associated with grapevines, tentative reservoir plants (Urtica dioica and Convolvulus arvensis) and the H. obsoletus associated with these plants. Among the 91 'Ca. P. solani' strains detected in diverse plant and insect hosts, three tuf, five vmp1 and 11 distinct stamp genotypes were identified. Twelve comprehensive genotypes of 'Ca. P. solani' were detected according to the tuf/vmp1/stamp genotyping. The highest diversity of genotypes was detected among the strains from H. obsoletus individuals associated with U. dioica, of which the most frequent genotype was tuf-ab/V18/M1 (43 %). The tuf-b/V2-TA/STOL comprehensive genotype was found in 33 % of naturally infected grapevines. Two 'Ca. P. solani' genotypes were associated with U. dioica, namely (i) tuf-ab/V18/M1 (60 %) and tuf-a/V3/M4 (40 %), and only one genotype (tuf-b/V2-TA/Rqg50) was associated with C. arvensis.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia", pages = "770-759", number = "4", volume = "142", doi = "10.1007/s10658-015-0649-0" }
Atanasova, B., Jakovljević, M., Spasov, D., Jović, J., Mitrović, M., Toševski, I.,& Cvrković, T.. (2015). The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology Springer, Dordrecht., 142(4), 759-770. https://doi.org/10.1007/s10658-015-0649-0
Atanasova B, Jakovljević M, Spasov D, Jović J, Mitrović M, Toševski I, Cvrković T. The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia. in European Journal of Plant Pathology. 2015;142(4):759-770. doi:10.1007/s10658-015-0649-0 .
Atanasova, Biljana, Jakovljević, Miljana, Spasov, Dušan, Jović, Jelena, Mitrović, Milana, Toševski, Ivo, Cvrković, Tatjana, "The molecular epidemiology of bois noir grapevine yellows caused by 'Candidatus Phytoplasma solani' in the Republic of Macedonia" in European Journal of Plant Pathology, 142, no. 4 (2015):759-770, https://doi.org/10.1007/s10658-015-0649-0 . .