DTBA and ELISA methods in detection of grapevine leafroll-1 virus
Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1
2006
Аутори
Ivanović, ŽarkoKuzmanović, Slobodan
Trkulja, Nenad
Živković, Svetlana
Stojanović, Saša
Starović, Mira
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue.
Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.
Кључне речи:
grapevine / viruses / ELISA / tissue blot / vinova loza / vizuelni test / ELISA test / 'tissue blot' testИзвор:
Zaštita bilja, 2006, 57, 1-4, 69-79Издавач:
- Institute for Plant Protection and Environment, Belgrade
Институција/група
IZBISTY - JOUR AU - Ivanović, Žarko AU - Kuzmanović, Slobodan AU - Trkulja, Nenad AU - Živković, Svetlana AU - Stojanović, Saša AU - Starović, Mira PY - 2006 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/40 AB - Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue. AB - Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze. PB - Institute for Plant Protection and Environment, Belgrade T2 - Zaštita bilja T1 - DTBA and ELISA methods in detection of grapevine leafroll-1 virus T1 - Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1 EP - 79 IS - 1-4 SP - 69 VL - 57 ER -
@article{ author = "Ivanović, Žarko and Kuzmanović, Slobodan and Trkulja, Nenad and Živković, Svetlana and Stojanović, Saša and Starović, Mira", year = "2006", abstract = "Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue., Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze.", publisher = "Institute for Plant Protection and Environment, Belgrade", journal = "Zaštita bilja", title = "DTBA and ELISA methods in detection of grapevine leafroll-1 virus, Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1", pages = "79-69", number = "1-4", volume = "57" }
Ivanović, Ž., Kuzmanović, S., Trkulja, N., Živković, S., Stojanović, S.,& Starović, M.. (2006). DTBA and ELISA methods in detection of grapevine leafroll-1 virus. in Zaštita bilja Institute for Plant Protection and Environment, Belgrade., 57(1-4), 69-79.
Ivanović Ž, Kuzmanović S, Trkulja N, Živković S, Stojanović S, Starović M. DTBA and ELISA methods in detection of grapevine leafroll-1 virus. in Zaštita bilja. 2006;57(1-4):69-79..
Ivanović, Žarko, Kuzmanović, Slobodan, Trkulja, Nenad, Živković, Svetlana, Stojanović, Saša, Starović, Mira, "DTBA and ELISA methods in detection of grapevine leafroll-1 virus" in Zaštita bilja, 57, no. 1-4 (2006):69-79.