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DTBA and ELISA methods in detection of grapevine leafroll-1 virus
Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1
dc.creator | Ivanović, Žarko | |
dc.creator | Kuzmanović, Slobodan | |
dc.creator | Trkulja, Nenad | |
dc.creator | Živković, Svetlana | |
dc.creator | Stojanović, Saša | |
dc.creator | Starović, Mira | |
dc.date.accessioned | 2019-04-04T15:51:19Z | |
dc.date.available | 2019-04-04T15:51:19Z | |
dc.date.issued | 2006 | |
dc.identifier.issn | 0372-7866 | |
dc.identifier.uri | https://plantarum.izbis.bg.ac.rs/handle/123456789/40 | |
dc.description.abstract | Grapevine leafroll syndrome is caused by a complex of up to nine different Grapevine leafroll-associated viruses (GLRaV-1-9). Many methods, including indexing, serological and molecular procedures, have been developed for the detection of GLRaV. However, due to the low concentration of the virus in plants a method with improved sensitivity and with the capacity to detect is required. In this study plants were tested for Grapevine leafroll virus (GLRaV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Tissues were homogenized for use in a double antibody sandwich ELISA system and then blotted on a nitrocellulose membrane for DTBA. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting GLRaV-1 in leaf tissue. | en |
dc.description.abstract | Sindrom uvijanja lista vinove loze je oboljenje koje prouzrokuje grupa virusa koja se naziva Virusi vezani za uvijanje lista vinove loze (GLRaV-1-9). Više različitih metoda je razvijeno za detekciju ove grupe virusa, kao što su indeksiranje, serološke i molekularno biološke metode. Zbog relativno male koncentracije virusa u zaraženoj biljci potrebno je pronaći metodu koja ima najveću osetljivost pri njegovoj detekciji. U ovom radu u cilju detektovanja virusa GLRaV, ispitivane biljke su pored vizuelnog pregleda obrađivane i pomoću ELISA i DTBA metoda. Poređenjem ELISA i DTBA metode na simptomatičnim biljkama utvrđeno je da se ove dve metode sa jednakom tačnošću mogu koristiti u otkrivanju GLRaV-1 u listu vinove loze. | sr |
dc.publisher | Institute for Plant Protection and Environment, Belgrade | |
dc.rights | openAccess | |
dc.source | Zaštita bilja | |
dc.subject | grapevine | en |
dc.subject | viruses | en |
dc.subject | ELISA | en |
dc.subject | tissue blot | en |
dc.subject | vinova loza | sr |
dc.subject | vizuelni test | sr |
dc.subject | ELISA test | sr |
dc.subject | 'tissue blot' test | sr |
dc.title | DTBA and ELISA methods in detection of grapevine leafroll-1 virus | en |
dc.title | Primena DTBA i ELISA metode u dokazivanju virusa uvijenosti lista vinove loze-1 | sr |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 79 | |
dc.citation.issue | 1-4 | |
dc.citation.other | 57(1-4): 69-79 | |
dc.citation.spage | 69 | |
dc.citation.volume | 57 | |
dc.type.version | publishedVersion | |
dc.identifier.fulltext | https://plantarum.izbis.bg.ac.rs//bitstream/id/1525/38.pdf |