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Prvi nalaz tumorogenih sojeva Agrobacterium radiobacter na malini u Srbiji

dc.creatorMilijašević, Svetlana
dc.creatorGavrilović, Veljko
dc.creatorŽivković, Svetlana
dc.creatorTrkulja, Nenad
dc.creatorPulawska, Joanna
dc.date.accessioned2019-04-04T15:51:44Z
dc.date.available2019-04-04T15:51:44Z
dc.date.issued2007
dc.identifier.issn1820-3949
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/53
dc.description.abstractDuring the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemical and physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 gene located on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia.en
dc.description.abstractTokom proleća 2003. godine, zapaženi su tumori na korenu i korenovom vratu mladih biljaka maline sorte Vilamette u okolini Valjeva. Iz obolelih uzoraka izolovane su fitopatogene bakterije. Proučavanjem patogenih, morfoloških i diferencijalnih biohemijsko-fizioloških karakteristika izolovanih sojeva, utvrđeno je da proučavani sojevi pripadaju bakteriji Agrobacterium radiobacter (biovar 1). U cilju potvrde identiteta izolovanih sojeva lančanom reakcijom polimeraze (PCR), korišćeni su prajmeri komplementarni tms2 genu lociranom na Ti plazmidu. U prvoj PCR reakciji izvedenoj korišćenjem tms2F1 + tms2R2 para prajmera, detektovani su fragmenti nukleinske kiseline veličine 617 baznih parova (bp), specifični za tumorogene sojeve A. tumefaciens. U drugoj PCR reakciji korišćenjem para prajmera tms2F1 i tms2B su amplifikovani PCR produkti očekivane veličine 458 bp, karakteristični za ovu bakteriju. Na osnovu multiplex PCR reakcije korišćenjem prajmera komplementarnih na hromozomalni gen koji kodira 23S rRNA sojevi izolovani iz tumora na korenu maline svrstani su u biovar 1 Agrobacterium (A. radiobacter). Ovo je prvi nalaz ove bakterije na malini u Srbiji.sr
dc.publisherInstitute of Pesticides and Environmental Protection
dc.rightsopenAccess
dc.sourcePesticidi i fitomedicina
dc.subjecttumorigenic agrobacteriumen
dc.subjectcrown gallen
dc.subjectraspberryen
dc.subjectidentificationen
dc.subjectPCRen
dc.subjecttumorogeni sojevisr
dc.subjectagrobacteriumsr
dc.subjectbakteriozni rak korenasr
dc.subjectmalinasr
dc.subjectidentifikacijasr
dc.subjectPCRsr
dc.titleFirst report of tumorigenic Agrobacterium radiobacter on raspberry in Serbiaen
dc.titlePrvi nalaz tumorogenih sojeva Agrobacterium radiobacter na malini u Srbijisr
dc.typearticle
dc.rights.licenseBY-SA
dc.citation.epage119
dc.citation.issue2
dc.citation.other22(2): 113-119
dc.citation.spage113
dc.citation.volume22
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1611/51.pdf
dc.identifier.rcubconv_31


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