Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry
Abstract
A method for the direct determination of pefloxacin in serum and pharmaceutical forms (tablets and ampoules) has been developed, based on the use of second-order derivative ultraviolet spectra. Spectrophotometric assay of pefloxacin in tablets and ampoules was carried out in 0.1 mol/L NaOH, while in serum it was performed in 0.1 mol/L NaOH with the addition of sodium dodecylsulfate, in 337-347 nm wavelength range. Linear calibration curves were obtained in the concentration ranges 2-30μg/mL pefloxacin for tablets and ampoules and 0.12-5μg/ mL for serum samples. Relative error of determination, as criterion for accuracy, was less than 1%, while the precision was better than 4ng/ml. The minimum detectable concentration of pefloxacin in serum was 15ng/mL.
Keywords:
Derivative spectrophotometry / Pefloxacin / Pharmaceutical forms / SerumSource:
Mikrochimica Acta, 1997, 126, 3, 203-206Publisher:
- Springer Verlag
DOI: 10.1007/BF01242321
ISSN: 0026-3672
WoS: A1997XG63500003
Scopus: 2-s2.0-0012501004
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IZBISTY - JOUR AU - Đurđević, P. AU - Jelikic-Stankov, M. AU - Milićević, Zoran PY - 1997 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/6 AB - A method for the direct determination of pefloxacin in serum and pharmaceutical forms (tablets and ampoules) has been developed, based on the use of second-order derivative ultraviolet spectra. Spectrophotometric assay of pefloxacin in tablets and ampoules was carried out in 0.1 mol/L NaOH, while in serum it was performed in 0.1 mol/L NaOH with the addition of sodium dodecylsulfate, in 337-347 nm wavelength range. Linear calibration curves were obtained in the concentration ranges 2-30μg/mL pefloxacin for tablets and ampoules and 0.12-5μg/ mL for serum samples. Relative error of determination, as criterion for accuracy, was less than 1%, while the precision was better than 4ng/ml. The minimum detectable concentration of pefloxacin in serum was 15ng/mL. PB - Springer Verlag T2 - Mikrochimica Acta T1 - Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry EP - 206 IS - 3 SP - 203 VL - 126 DO - 10.1007/BF01242321 ER -
@article{ author = "Đurđević, P. and Jelikic-Stankov, M. and Milićević, Zoran", year = "1997", abstract = "A method for the direct determination of pefloxacin in serum and pharmaceutical forms (tablets and ampoules) has been developed, based on the use of second-order derivative ultraviolet spectra. Spectrophotometric assay of pefloxacin in tablets and ampoules was carried out in 0.1 mol/L NaOH, while in serum it was performed in 0.1 mol/L NaOH with the addition of sodium dodecylsulfate, in 337-347 nm wavelength range. Linear calibration curves were obtained in the concentration ranges 2-30μg/mL pefloxacin for tablets and ampoules and 0.12-5μg/ mL for serum samples. Relative error of determination, as criterion for accuracy, was less than 1%, while the precision was better than 4ng/ml. The minimum detectable concentration of pefloxacin in serum was 15ng/mL.", publisher = "Springer Verlag", journal = "Mikrochimica Acta", title = "Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry", pages = "206-203", number = "3", volume = "126", doi = "10.1007/BF01242321" }
Đurđević, P., Jelikic-Stankov, M.,& Milićević, Z.. (1997). Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry. in Mikrochimica Acta Springer Verlag., 126(3), 203-206. https://doi.org/10.1007/BF01242321
Đurđević P, Jelikic-Stankov M, Milićević Z. Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry. in Mikrochimica Acta. 1997;126(3):203-206. doi:10.1007/BF01242321 .
Đurđević, P., Jelikic-Stankov, M., Milićević, Zoran, "Determination of Pefloxacin in Serum and Pharmaceutical Forms by Derivative Spectrophotometry" in Mikrochimica Acta, 126, no. 3 (1997):203-206, https://doi.org/10.1007/BF01242321 . .