On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology
Само за регистроване кориснике
2011
Аутори
Kos, KatarinaPetrović, Anđeljko
Stary, Petr
Kavallieratos, Nickolas G.
Ivanović, Ana
Toševski, Ivo
Jakse, J
Trdan, Stanislav
Tomanović, Željko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, the relationships among and the taxonomic status of three closely related parasitic wasps that are widely used as biological control agents of cereal aphids, Aphidius uzbekistanicus Luzhetzki, Aphidius rhopalosiphi De Stefani Perez, and Aphidius avenaphis (Fitch), were examined. Genetic divergence at an average of 6% was recorded between A. uzbekistanicus and A. rhopalosiphi by using the mitochondrial (mt) gene cytochrome oxidase I (COI) barcoding region. Identical mtCOI gene sequences were observed in A. uzbekistanicus specimens that originated from Eurasia and in the North American species A. avenaphis. The haplotype fluctuation in A. rhopalosiphi specimens that originated from the west Palaearctic was an average of 1.5% (maximum, 2.4%). In contrast, specimens of A. uzbekistanicus from central and western parts of Eurasia were largely homogenous, with only a single mutation recorded in a specimen from eastern Europe (Serbia). The morphological and genetic diversity fou...nd in A. rhopalosiphi may suggest the existence of cryptic species, especially for lineages that have a large degree of mtCOI diversity and sympatric occurrence. The geometric morphometric analysis of stigma shape presented in this study demonstrated that members of A. uzbekistanicus have a shorter forewing r vein and a more elongated stigma, relative to those of A. avenaphis. Our research validates the use of stigma shape and flagellomere 1 color for morphological discrimination between wasp species.
Кључне речи:
aphidiine wasps / morphological / genetic diversity / barcodingИзвор:
Annals of the Entomological Society of America, 2011, 104, 6, 1221-1232Издавач:
- Entomological Soc Amer, Lanham
Финансирање / пројекти:
- Агробиодиверзитет и коришћење земљишта у Србији: интегрисана процена биодиверзитета кључних група артропода и биљних патогена (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
- Grant Agency of the Czech Republic from the Entomology Institute (Academy of Sciences of the Czech Republic) - 522/09/1940
- Grant Agency of the Czech Republic from the Entomology Institute (Academy of Sciences of the Czech Republic) - AVOZ50070508
- Slovenian Research Agency - P4-0013
DOI: 10.1603/AN11055
ISSN: 0013-8746
WoS: 000297163900013
Scopus: 2-s2.0-82155170548
Институција/група
IZBISTY - JOUR AU - Kos, Katarina AU - Petrović, Anđeljko AU - Stary, Petr AU - Kavallieratos, Nickolas G. AU - Ivanović, Ana AU - Toševski, Ivo AU - Jakse, J AU - Trdan, Stanislav AU - Tomanović, Željko PY - 2011 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/169 AB - In this study, the relationships among and the taxonomic status of three closely related parasitic wasps that are widely used as biological control agents of cereal aphids, Aphidius uzbekistanicus Luzhetzki, Aphidius rhopalosiphi De Stefani Perez, and Aphidius avenaphis (Fitch), were examined. Genetic divergence at an average of 6% was recorded between A. uzbekistanicus and A. rhopalosiphi by using the mitochondrial (mt) gene cytochrome oxidase I (COI) barcoding region. Identical mtCOI gene sequences were observed in A. uzbekistanicus specimens that originated from Eurasia and in the North American species A. avenaphis. The haplotype fluctuation in A. rhopalosiphi specimens that originated from the west Palaearctic was an average of 1.5% (maximum, 2.4%). In contrast, specimens of A. uzbekistanicus from central and western parts of Eurasia were largely homogenous, with only a single mutation recorded in a specimen from eastern Europe (Serbia). The morphological and genetic diversity found in A. rhopalosiphi may suggest the existence of cryptic species, especially for lineages that have a large degree of mtCOI diversity and sympatric occurrence. The geometric morphometric analysis of stigma shape presented in this study demonstrated that members of A. uzbekistanicus have a shorter forewing r vein and a more elongated stigma, relative to those of A. avenaphis. Our research validates the use of stigma shape and flagellomere 1 color for morphological discrimination between wasp species. PB - Entomological Soc Amer, Lanham T2 - Annals of the Entomological Society of America T1 - On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology EP - 1232 IS - 6 SP - 1221 VL - 104 DO - 10.1603/AN11055 ER -
@article{ author = "Kos, Katarina and Petrović, Anđeljko and Stary, Petr and Kavallieratos, Nickolas G. and Ivanović, Ana and Toševski, Ivo and Jakse, J and Trdan, Stanislav and Tomanović, Željko", year = "2011", abstract = "In this study, the relationships among and the taxonomic status of three closely related parasitic wasps that are widely used as biological control agents of cereal aphids, Aphidius uzbekistanicus Luzhetzki, Aphidius rhopalosiphi De Stefani Perez, and Aphidius avenaphis (Fitch), were examined. Genetic divergence at an average of 6% was recorded between A. uzbekistanicus and A. rhopalosiphi by using the mitochondrial (mt) gene cytochrome oxidase I (COI) barcoding region. Identical mtCOI gene sequences were observed in A. uzbekistanicus specimens that originated from Eurasia and in the North American species A. avenaphis. The haplotype fluctuation in A. rhopalosiphi specimens that originated from the west Palaearctic was an average of 1.5% (maximum, 2.4%). In contrast, specimens of A. uzbekistanicus from central and western parts of Eurasia were largely homogenous, with only a single mutation recorded in a specimen from eastern Europe (Serbia). The morphological and genetic diversity found in A. rhopalosiphi may suggest the existence of cryptic species, especially for lineages that have a large degree of mtCOI diversity and sympatric occurrence. The geometric morphometric analysis of stigma shape presented in this study demonstrated that members of A. uzbekistanicus have a shorter forewing r vein and a more elongated stigma, relative to those of A. avenaphis. Our research validates the use of stigma shape and flagellomere 1 color for morphological discrimination between wasp species.", publisher = "Entomological Soc Amer, Lanham", journal = "Annals of the Entomological Society of America", title = "On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology", pages = "1232-1221", number = "6", volume = "104", doi = "10.1603/AN11055" }
Kos, K., Petrović, A., Stary, P., Kavallieratos, N. G., Ivanović, A., Toševski, I., Jakse, J., Trdan, S.,& Tomanović, Ž.. (2011). On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology. in Annals of the Entomological Society of America Entomological Soc Amer, Lanham., 104(6), 1221-1232. https://doi.org/10.1603/AN11055
Kos K, Petrović A, Stary P, Kavallieratos NG, Ivanović A, Toševski I, Jakse J, Trdan S, Tomanović Ž. On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology. in Annals of the Entomological Society of America. 2011;104(6):1221-1232. doi:10.1603/AN11055 .
Kos, Katarina, Petrović, Anđeljko, Stary, Petr, Kavallieratos, Nickolas G., Ivanović, Ana, Toševski, Ivo, Jakse, J, Trdan, Stanislav, Tomanović, Željko, "On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology" in Annals of the Entomological Society of America, 104, no. 6 (2011):1221-1232, https://doi.org/10.1603/AN11055 . .