Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation
2015
Autori
Martinović, Vesna I.Ivanović, Žarko
Mihailović, Mirjana
Ivanović-Matić, Svetlana K.
Poznanović, Goran D.
Vidaković, Melita S.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We examined the functions of the rat lymphocyte nuclear matrix after a single exposure to total body irradiation with doses ranging from sublethal to lethal. Irradiation induced systemic oxidative stress, detected as increased activities of serum SOD and catalase, lymphocyte DNA damage, detected by the Comet assay, and apoptosis. After irradiation with lower doses, the recruitment of DNA repair centers on the matrix was observed by Western analysis as increased levels of matrix-associated PARP-1, p53 and PCNA. Augmented partitioning of the pro-survival transcription factor NF-kappa B on the matrix was also detected after irradiation. Exposure to a lethal dose caused breakdown of the matrix, observed as lamin B cleavage, and of the matrix-associated DNA repair centers, detected as caspase-mediated PARP-1 proteolysis and loss of protein associations with the matrix. These findings suggest that the nuclear matrix establishes functional interactions in a defensive mechanism, integrated in ...a decision-making process that resolves cell fate.
Ključne reči:
DNA repair / ionizing radiation / NF-kappa B / nuclear matrix / p53 / PARP-1 / PCNAIzvor:
Archives of Biological Sciences, 2015, 67, 1, 69-81Izdavač:
- University of Belgrade, University of Novi Sad
Finansiranje / projekti:
- Signalni molekuli u dijabetesu: identifikacija potencijalnih bioloških markera uključenih u modifikaciju i integraciju signalnih puteva u cilju predikcije i intervencije u dijabetesu (RS-MESTD-Basic Research (BR or ON)-173020)
DOI: 10.2298/ABS140124002M
ISSN: 0354-4664
WoS: 000352003500008
Scopus: 2-s2.0-84937441539
Institucija/grupa
IZBISTY - JOUR AU - Martinović, Vesna I. AU - Ivanović, Žarko AU - Mihailović, Mirjana AU - Ivanović-Matić, Svetlana K. AU - Poznanović, Goran D. AU - Vidaković, Melita S. PY - 2015 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/389 AB - We examined the functions of the rat lymphocyte nuclear matrix after a single exposure to total body irradiation with doses ranging from sublethal to lethal. Irradiation induced systemic oxidative stress, detected as increased activities of serum SOD and catalase, lymphocyte DNA damage, detected by the Comet assay, and apoptosis. After irradiation with lower doses, the recruitment of DNA repair centers on the matrix was observed by Western analysis as increased levels of matrix-associated PARP-1, p53 and PCNA. Augmented partitioning of the pro-survival transcription factor NF-kappa B on the matrix was also detected after irradiation. Exposure to a lethal dose caused breakdown of the matrix, observed as lamin B cleavage, and of the matrix-associated DNA repair centers, detected as caspase-mediated PARP-1 proteolysis and loss of protein associations with the matrix. These findings suggest that the nuclear matrix establishes functional interactions in a defensive mechanism, integrated in a decision-making process that resolves cell fate. PB - University of Belgrade, University of Novi Sad T2 - Archives of Biological Sciences T1 - Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation EP - 81 IS - 1 SP - 69 VL - 67 DO - 10.2298/ABS140124002M ER -
@article{ author = "Martinović, Vesna I. and Ivanović, Žarko and Mihailović, Mirjana and Ivanović-Matić, Svetlana K. and Poznanović, Goran D. and Vidaković, Melita S.", year = "2015", abstract = "We examined the functions of the rat lymphocyte nuclear matrix after a single exposure to total body irradiation with doses ranging from sublethal to lethal. Irradiation induced systemic oxidative stress, detected as increased activities of serum SOD and catalase, lymphocyte DNA damage, detected by the Comet assay, and apoptosis. After irradiation with lower doses, the recruitment of DNA repair centers on the matrix was observed by Western analysis as increased levels of matrix-associated PARP-1, p53 and PCNA. Augmented partitioning of the pro-survival transcription factor NF-kappa B on the matrix was also detected after irradiation. Exposure to a lethal dose caused breakdown of the matrix, observed as lamin B cleavage, and of the matrix-associated DNA repair centers, detected as caspase-mediated PARP-1 proteolysis and loss of protein associations with the matrix. These findings suggest that the nuclear matrix establishes functional interactions in a defensive mechanism, integrated in a decision-making process that resolves cell fate.", publisher = "University of Belgrade, University of Novi Sad", journal = "Archives of Biological Sciences", title = "Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation", pages = "81-69", number = "1", volume = "67", doi = "10.2298/ABS140124002M" }
Martinović, V. I., Ivanović, Ž., Mihailović, M., Ivanović-Matić, S. K., Poznanović, G. D.,& Vidaković, M. S.. (2015). Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation. in Archives of Biological Sciences University of Belgrade, University of Novi Sad., 67(1), 69-81. https://doi.org/10.2298/ABS140124002M
Martinović VI, Ivanović Ž, Mihailović M, Ivanović-Matić SK, Poznanović GD, Vidaković MS. Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation. in Archives of Biological Sciences. 2015;67(1):69-81. doi:10.2298/ABS140124002M .
Martinović, Vesna I., Ivanović, Žarko, Mihailović, Mirjana, Ivanović-Matić, Svetlana K., Poznanović, Goran D., Vidaković, Melita S., "Lymphocytes' 'last stand' on the nuclear matrix after whole body exposure of rats to low-let ionizing radiation" in Archives of Biological Sciences, 67, no. 1 (2015):69-81, https://doi.org/10.2298/ABS140124002M . .