Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro
2017
Authors
Ivanović, ŽarkoPerović, Tatjana
Popović, Tatjana
Blagojević, Jovana
Trkulja, Nenad
Hrnčić, Snježana
Article (Published version)
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Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gapl gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gapl gene sequences showed that P syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a ...possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P syringae control.
Keywords:
bacteria / Citrus reticulata / multilocus sequence analysis / pathogen / phylogenySource:
Plant Pathology Journal, 2017, 33, 1, 21-33Publisher:
- Hanrimwon Publishing Company
Funding / projects:
- Development of integrated approach in plant protection for control harmful organisms (RS-MESTD-Technological Development (TD or TR)-31018)
- Ministry of Science, Montenegro (Grant "Invasive species")
DOI: 10.5423/PPJ.OA.08.2016.0161
ISSN: 1598-2254
PubMed: 28167885
WoS: 000393271100003
Scopus: 2-s2.0-85011579682
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IZBISTY - JOUR AU - Ivanović, Žarko AU - Perović, Tatjana AU - Popović, Tatjana AU - Blagojević, Jovana AU - Trkulja, Nenad AU - Hrnčić, Snježana PY - 2017 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/474 AB - Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gapl gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gapl gene sequences showed that P syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P syringae control. PB - Hanrimwon Publishing Company T2 - Plant Pathology Journal T1 - Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro EP - 33 IS - 1 SP - 21 VL - 33 DO - 10.5423/PPJ.OA.08.2016.0161 ER -
@article{ author = "Ivanović, Žarko and Perović, Tatjana and Popović, Tatjana and Blagojević, Jovana and Trkulja, Nenad and Hrnčić, Snježana", year = "2017", abstract = "Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gapl gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gapl gene sequences showed that P syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P syringae control.", publisher = "Hanrimwon Publishing Company", journal = "Plant Pathology Journal", title = "Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro", pages = "33-21", number = "1", volume = "33", doi = "10.5423/PPJ.OA.08.2016.0161" }
Ivanović, Ž., Perović, T., Popović, T., Blagojević, J., Trkulja, N.,& Hrnčić, S.. (2017). Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro. in Plant Pathology Journal Hanrimwon Publishing Company., 33(1), 21-33. https://doi.org/10.5423/PPJ.OA.08.2016.0161
Ivanović Ž, Perović T, Popović T, Blagojević J, Trkulja N, Hrnčić S. Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro. in Plant Pathology Journal. 2017;33(1):21-33. doi:10.5423/PPJ.OA.08.2016.0161 .
Ivanović, Žarko, Perović, Tatjana, Popović, Tatjana, Blagojević, Jovana, Trkulja, Nenad, Hrnčić, Snježana, "Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro" in Plant Pathology Journal, 33, no. 1 (2017):21-33, https://doi.org/10.5423/PPJ.OA.08.2016.0161 . .