Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.
Аутори
Einspanier, SeverinSusanto, Tamara
Metz, Nicole
Wolters, Pieter
Vleeshouwers, Vivianne
Lankinen, Åsa
Liljeroth, Erland
Landschoot, Sofie
Ivanović, Žarko
Huckelhoven, Ralph
Hausladen, Hans
Stam, Remco
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread through...out Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds.
Кључне речи:
agriculture / Alternaria solani / fungicide resistance / plant pathology / population genetics –empirical / potatoИзвор:
Evolutionary Applications, 2022, 15, 10, 1605-1620Издавач:
- Wiley
DOI: 10.1111/eva.13350
ISSN: 1752-4571
WoS: 000758930600001
Scopus: 2-s2.0-85125759993
Институција/група
IZBISTY - JOUR AU - Einspanier, Severin AU - Susanto, Tamara AU - Metz, Nicole AU - Wolters, Pieter AU - Vleeshouwers, Vivianne AU - Lankinen, Åsa AU - Liljeroth, Erland AU - Landschoot, Sofie AU - Ivanović, Žarko AU - Huckelhoven, Ralph AU - Hausladen, Hans AU - Stam, Remco PY - 2022 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/745 AB - Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread throughout Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds. PB - Wiley T2 - Evolutionary Applications T1 - Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani. EP - 1620 IS - 10 SP - 1605 VL - 15 DO - 10.1111/eva.13350 ER -
@article{ author = "Einspanier, Severin and Susanto, Tamara and Metz, Nicole and Wolters, Pieter and Vleeshouwers, Vivianne and Lankinen, Åsa and Liljeroth, Erland and Landschoot, Sofie and Ivanović, Žarko and Huckelhoven, Ralph and Hausladen, Hans and Stam, Remco", year = "2022", abstract = "Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread throughout Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds.", publisher = "Wiley", journal = "Evolutionary Applications", title = "Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.", pages = "1620-1605", number = "10", volume = "15", doi = "10.1111/eva.13350" }
Einspanier, S., Susanto, T., Metz, N., Wolters, P., Vleeshouwers, V., Lankinen, Å., Liljeroth, E., Landschoot, S., Ivanović, Ž., Huckelhoven, R., Hausladen, H.,& Stam, R.. (2022). Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.. in Evolutionary Applications Wiley., 15(10), 1605-1620. https://doi.org/10.1111/eva.13350
Einspanier S, Susanto T, Metz N, Wolters P, Vleeshouwers V, Lankinen Å, Liljeroth E, Landschoot S, Ivanović Ž, Huckelhoven R, Hausladen H, Stam R. Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani.. in Evolutionary Applications. 2022;15(10):1605-1620. doi:10.1111/eva.13350 .
Einspanier, Severin, Susanto, Tamara, Metz, Nicole, Wolters, Pieter, Vleeshouwers, Vivianne, Lankinen, Åsa, Liljeroth, Erland, Landschoot, Sofie, Ivanović, Žarko, Huckelhoven, Ralph, Hausladen, Hans, Stam, Remco, "Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani." in Evolutionary Applications, 15, no. 10 (2022):1605-1620, https://doi.org/10.1111/eva.13350 . .