Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results
Аутори
Nešič, KsenijaPisinov, Boris
Jakšić, Sandra
Tasić, Aleksandra
Savić, Božidar
Pavlović, Nikola
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Also, rapid methods for mycotoxin analysis have become increasingly important. Enzyme-linked immuno-sorbent assay (ELISA) is one of the most common rapid methods for determination of these natural contaminants. The aim of this study was to provide a comparison between two different methods of analysis (HPLC and ELISA) for the detection of different mycotoxins using data that originate from commercial proficiency tests. Based on the statistical evaluation of the results for both methods, in three proficiency tests for various mycotoxins (aflatoxins, ochratoxin and zearalenone), it could be concluded that both techniques can equally be used, altho...ugh ELISA is considered to be the screening one.
Извор:
Zbornik Matice srpske za prirodne nauke, 2017, 133, 79-93Издавач:
- Matica Srpska
Финансирање / пројекти:
- nfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46009/RS//
Институција/група
IZBISTY - JOUR AU - Nešič, Ksenija AU - Pisinov, Boris AU - Jakšić, Sandra AU - Tasić, Aleksandra AU - Savić, Božidar AU - Pavlović, Nikola PY - 2017 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/990 AB - Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Also, rapid methods for mycotoxin analysis have become increasingly important. Enzyme-linked immuno-sorbent assay (ELISA) is one of the most common rapid methods for determination of these natural contaminants. The aim of this study was to provide a comparison between two different methods of analysis (HPLC and ELISA) for the detection of different mycotoxins using data that originate from commercial proficiency tests. Based on the statistical evaluation of the results for both methods, in three proficiency tests for various mycotoxins (aflatoxins, ochratoxin and zearalenone), it could be concluded that both techniques can equally be used, although ELISA is considered to be the screening one. PB - Matica Srpska T2 - Zbornik Matice srpske za prirodne nauke T1 - Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results EP - 93 SP - 79 VL - 133 DO - 10.2298/ZMSPN1733079N ER -
@article{ author = "Nešič, Ksenija and Pisinov, Boris and Jakšić, Sandra and Tasić, Aleksandra and Savić, Božidar and Pavlović, Nikola", year = "2017", abstract = "Different analytical techniques for the detection of mycotoxins have been developed in order to control the levels of mycotoxins in food and feed. Conventional analytical methods for mycotoxin determination are involving techniques such as thinlayer chromatography (TLC), high-performance liquid chromatography (HPLC) and gas chromatography (GC). Also, rapid methods for mycotoxin analysis have become increasingly important. Enzyme-linked immuno-sorbent assay (ELISA) is one of the most common rapid methods for determination of these natural contaminants. The aim of this study was to provide a comparison between two different methods of analysis (HPLC and ELISA) for the detection of different mycotoxins using data that originate from commercial proficiency tests. Based on the statistical evaluation of the results for both methods, in three proficiency tests for various mycotoxins (aflatoxins, ochratoxin and zearalenone), it could be concluded that both techniques can equally be used, although ELISA is considered to be the screening one.", publisher = "Matica Srpska", journal = "Zbornik Matice srpske za prirodne nauke", title = "Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results", pages = "93-79", volume = "133", doi = "10.2298/ZMSPN1733079N" }
Nešič, K., Pisinov, B., Jakšić, S., Tasić, A., Savić, B.,& Pavlović, N.. (2017). Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results. in Zbornik Matice srpske za prirodne nauke Matica Srpska., 133, 79-93. https://doi.org/10.2298/ZMSPN1733079N
Nešič K, Pisinov B, Jakšić S, Tasić A, Savić B, Pavlović N. Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results. in Zbornik Matice srpske za prirodne nauke. 2017;133:79-93. doi:10.2298/ZMSPN1733079N .
Nešič, Ksenija, Pisinov, Boris, Jakšić, Sandra, Tasić, Aleksandra, Savić, Božidar, Pavlović, Nikola, "Comparison of elisa and HPLC methods for the detection of mycotoxins by analysing proficiency test results" in Zbornik Matice srpske za prirodne nauke, 133 (2017):79-93, https://doi.org/10.2298/ZMSPN1733079N . .