TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/460
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institute of field and vegetable crops, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institute of field and vegetable crops, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}

Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo
Institute of field and vegetable crops, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984

Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Milovanović, Predrag
AU  - Aleksić, Goran
AU  - Gavrilović, Veljko
AU  - Starović, Mira
AU  - Vasić, Mirjana
AU  - Balaž, Jelica
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/209
AB  - Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.
PB  - Universidade de São Paulo
T2  - Scientia Agricola
T1  - Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds
EP  - 270
IS  - 4
SP  - 265
VL  - 69
DO  - 10.1590/S0103-90162012000400005
ER  - 

@article{
author = "Popović, Tatjana and Milovanović, Predrag and Aleksić, Goran and Gavrilović, Veljko and Starović, Mira and Vasić, Mirjana and Balaž, Jelica",
year = "2012",
abstract = "Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained strains was confirmed by the inoculation of germinated bean seed. The isolates recovered from the seed assay were further confirmed to be Psp by using both Enzyme-linked immunosorbent assay (ELISA) and Polymerase Chain Reaction (Nested-PCR) detection methodologies. The International Seed Testing Association (ISTA) method selected for this work was found to be effective in detecting the presence of Psp in common bean seed. The bacterium Psp was detected in only two of the 23 seed samples analyzed by this method, which shows that the bacterium is not widespread in Serbia.",
publisher = "Universidade de São Paulo",
journal = "Scientia Agricola",
title = "Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds",
pages = "270-265",
number = "4",
volume = "69",
doi = "10.1590/S0103-90162012000400005"
}

Popović, T., Milovanović, P., Aleksić, G., Gavrilović, V., Starović, M., Vasić, M.,& Balaž, J.. (2012). Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds. in Scientia Agricola
Universidade de São Paulo., 69(4), 265-270.
https://doi.org/10.1590/S0103-90162012000400005

Popović T, Milovanović P, Aleksić G, Gavrilović V, Starović M, Vasić M, Balaž J. Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds. in Scientia Agricola. 2012;69(4):265-270.
doi:10.1590/S0103-90162012000400005 .

Popović, Tatjana, Milovanović, Predrag, Aleksić, Goran, Gavrilović, Veljko, Starović, Mira, Vasić, Mirjana, Balaž, Jelica, "Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds" in Scientia Agricola, 69, no. 4 (2012):265-270,
https://doi.org/10.1590/S0103-90162012000400005 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Nikolić, Zorica
AU  - Starović, Mira
AU  - Gavrilović, Veljko
AU  - Aleksić, Goran
AU  - Vasić, Mirjana
AU  - Živković, Svetlana
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/123
AB  - Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.
PB  - Academic Journals, Victoria Island
T2  - African Journal of Agricultural Research
T1  - Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia
EP  - 2736
IS  - 19
SP  - 2730
VL  - 5
ER  - 

@article{
author = "Popović, Tatjana and Balaž, Jelica and Nikolić, Zorica and Starović, Mira and Gavrilović, Veljko and Aleksić, Goran and Vasić, Mirjana and Živković, Svetlana",
year = "2010",
abstract = "Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas campestris pv. phaseoli agar and milk tween agar were used to detect the bacterium from whole bean seed extract. Colonies of the bacterium were yellow, mucoid and convex on these media and zones of hydrolysis formed around them. The pathogenicity of the strains was confirmed by stem inoculation of young bean plants. Identification of the strains was proved by using rapid methods (ELISA and PCR). The results showed the presence of seed-borne X. axonopodis pv. phaseoli in 20 out of the 23 assayed bean cultivars that are commercially grown in Serbia. These results indicate that the seed of the bean cultivars grown in our country is highly infected by the bacterium.",
publisher = "Academic Journals, Victoria Island",
journal = "African Journal of Agricultural Research",
title = "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia",
pages = "2736-2730",
number = "19",
volume = "5"
}

Popović, T., Balaž, J., Nikolić, Z., Starović, M., Gavrilović, V., Aleksić, G., Vasić, M.,& Živković, S.. (2010). Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research
Academic Journals, Victoria Island., 5(19), 2730-2736.

Popović T, Balaž J, Nikolić Z, Starović M, Gavrilović V, Aleksić G, Vasić M, Živković S. Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia. in African Journal of Agricultural Research. 2010;5(19):2730-2736..

Popović, Tatjana, Balaž, Jelica, Nikolić, Zorica, Starović, Mira, Gavrilović, Veljko, Aleksić, Goran, Vasić, Mirjana, Živković, Svetlana, "Detection and identification of Xanthomonas axonopodis pv. phaseoli on bean seed collected in Serbia" in African Journal of Agricultural Research, 5, no. 19 (2010):2730-2736.

TY  - JOUR
AU  - Balaž, Jelica
AU  - Popović Milovanović, Tatjana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1066
AB  - U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja
EP  - 88
IS  - 2
SP  - 81
VL  - 23
DO  - 10.2298/PIF0802081B
ER  - 

@article{
author = "Balaž, Jelica and Popović Milovanović, Tatjana and Vasić, Mirjana and Nikolić, Zorica",
year = "2008",
abstract = "U radu je ispitivana mogućnost utvrđivanja prisustva bakterije Pseudomonas savastanoi pv. phaseolicola sa veštački inokulisanog semena pasulja. Korišćena je metoda International Seed Federation (ISF), koja uključuje ekstrakciju bakterija iz semena, izolaciju na poluselektivne podloge i proveru patogenosti dobijenih izolata. U cilju potvrde rezultata korišćene su brze savremene metode identifikacije (ELISA test i PCR). Rezultati dobijeni tokom rada pokazuju da su poluselektivne podloge MT (Milk Tween Agar) i MSP (Modified Sucrose Peptone Agar) pogodne za izolaciju ove bakterije. Patogenost odabranih izolata dokazana je na kotiledonim listovima pasulja. Primenom ELISA testova i PCR, potvrđeno je da svi ispitivani izolati i reizolati pripadaju bakteriji P.s.pv. phaseolicola.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja",
pages = "88-81",
number = "2",
volume = "23",
doi = "10.2298/PIF0802081B"
}

Balaž, J., Popović Milovanović, T., Vasić, M.,& Nikolić, Z.. (2008). Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 23(2), 81-88.
https://doi.org/10.2298/PIF0802081B

Balaž J, Popović Milovanović T, Vasić M, Nikolić Z. Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja. in Pesticidi i fitomedicina. 2008;23(2):81-88.
doi:10.2298/PIF0802081B .

Balaž, Jelica, Popović Milovanović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Razrada metoda za dokazivanje Pseudomonas savastanoi pv. phaseolicola na semenu pasulja" in Pesticidi i fitomedicina, 23, no. 2 (2008):81-88,
https://doi.org/10.2298/PIF0802081B . .

TY  - JOUR
AU  - Balaž, Jelica
AU  - Popović Milovanović, Tatjana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
PY  - 2008
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1063
AB  - method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds
EP  - 98
IS  - 2
SP  - 89
VL  - 23
DO  - 10.2298/PIF0802089B
ER  - 

@article{
author = "Balaž, Jelica and Popović Milovanović, Tatjana and Vasić, Mirjana and Nikolić, Zorica",
year = "2008",
abstract = "method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds",
pages = "98-89",
number = "2",
volume = "23",
doi = "10.2298/PIF0802089B"
}

Balaž, J., Popović Milovanović, T., Vasić, M.,& Nikolić, Z.. (2008). Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 23(2), 89-98.
https://doi.org/10.2298/PIF0802089B

Balaž J, Popović Milovanović T, Vasić M, Nikolić Z. Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina. 2008;23(2):89-98.
doi:10.2298/PIF0802089B .

Balaž, Jelica, Popović Milovanović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds" in Pesticidi i fitomedicina, 23, no. 2 (2008):89-98,
https://doi.org/10.2298/PIF0802089B . .