TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Poštić, Dobrivoj
AU  - Aleksić, Goran
AU  - Veljović Jovanović, Sonja
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/587
AB  - Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG5, REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant-Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip
EP  - 3082
IS  - 12
SP  - 3072
VL  - 103
DO  - 10.1094/PDIS-03-19-0674-RE
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Dimkić, Ivica and Stanković, Slaviša and Poštić, Dobrivoj and Aleksić, Goran and Veljović Jovanović, Sonja",
year = "2019",
abstract = "Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG5, REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant-Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip",
pages = "3082-3072",
number = "12",
volume = "103",
doi = "10.1094/PDIS-03-19-0674-RE"
}

Popović, T., Jelušić, A., Dimkić, I., Stanković, S., Poštić, D., Aleksić, G.,& Veljović Jovanović, S.. (2019). Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease
The American Phytopathological Society., 103(12), 3072-3082.
https://doi.org/10.1094/PDIS-03-19-0674-RE

Popović T, Jelušić A, Dimkić I, Stanković S, Poštić D, Aleksić G, Veljović Jovanović S. Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease. 2019;103(12):3072-3082.
doi:10.1094/PDIS-03-19-0674-RE .

Popović, Tatjana, Jelušić, Aleksandra, Dimkić, Ivica, Stanković, Slaviša, Poštić, Dobrivoj, Aleksić, Goran, Veljović Jovanović, Sonja, "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip" in Plant Disease, 103, no. 12 (2019):3072-3082,
https://doi.org/10.1094/PDIS-03-19-0674-RE . .

TY  - JOUR
AU  - Nikolić, I.
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Popović, Tatjana
AU  - Lozo, J.
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/577
AB  - Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.
PB  - Hoboken : Wiley
T2  - Journal of Applied Microbiology
T1  - Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains
EP  - 176
IS  - 1
SP  - 165
VL  - 126
DO  - 10.1111/jam.14070
ER  - 

@article{
author = "Nikolić, I. and Berić, Tanja and Dimkić, Ivica and Popović, Tatjana and Lozo, J. and Fira, Đorđe and Stanković, Slaviša",
year = "2019",
abstract = "Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.",
publisher = "Hoboken : Wiley",
journal = "Journal of Applied Microbiology",
title = "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains",
pages = "176-165",
number = "1",
volume = "126",
doi = "10.1111/jam.14070"
}

Nikolić, I., Berić, T., Dimkić, I., Popović, T., Lozo, J., Fira, Đ.,& Stanković, S.. (2019). Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology
Hoboken : Wiley., 126(1), 165-176.
https://doi.org/10.1111/jam.14070

Nikolić I, Berić T, Dimkić I, Popović T, Lozo J, Fira Đ, Stanković S. Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology. 2019;126(1):165-176.
doi:10.1111/jam.14070 .

Nikolić, I., Berić, Tanja, Dimkić, Ivica, Popović, Tatjana, Lozo, J., Fira, Đorđe, Stanković, Slaviša, "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains" in Journal of Applied Microbiology, 126, no. 1 (2019):165-176,
https://doi.org/10.1111/jam.14070 . .

TY  - JOUR
AU  - Nikolić, I.
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Stojšin, Vera
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/553
AB  - Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet
EP  - 1207
IS  - 5
SP  - 1194
VL  - 67
DO  - 10.1111/ppa.12831
ER  - 

@article{
author = "Nikolić, I. and Stanković, Slaviša and Dimkić, Ivica and Berić, Tanja and Stojšin, Vera and Janse, Jaap and Popović, Tatjana",
year = "2018",
abstract = "Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet",
pages = "1207-1194",
number = "5",
volume = "67",
doi = "10.1111/ppa.12831"
}

Nikolić, I., Stanković, S., Dimkić, I., Berić, T., Stojšin, V., Janse, J.,& Popović, T.. (2018). Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology
Wiley, Hoboken., 67(5), 1194-1207.
https://doi.org/10.1111/ppa.12831

Nikolić I, Stanković S, Dimkić I, Berić T, Stojšin V, Janse J, Popović T. Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology. 2018;67(5):1194-1207.
doi:10.1111/ppa.12831 .

Nikolić, I., Stanković, Slaviša, Dimkić, Ivica, Berić, Tanja, Stojšin, Vera, Janse, Jaap, Popović, Tatjana, "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet" in Plant Pathology, 67, no. 5 (2018):1194-1207,
https://doi.org/10.1111/ppa.12831 . .

Popović Milovanović, T., Jelušić, A., Milovanović, P., Janjatović, S., Budnar, M., Dimkić, I.,& Stanković, S.. (2017). First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease
American Phytopathological Society., 101(12), 2145-2146.
https://doi.org/10.1094/PDIS-05-17-0708-PDN

Popović Milovanović T, Jelušić A, Milovanović P, Janjatović S, Budnar M, Dimkić I, Stanković S. First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease. 2017;101(12):2145-2146.
doi:10.1094/PDIS-05-17-0708-PDN .

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Janjatović, S., Budnar, M., Dimkić, Ivica, Stanković, Slaviša, "First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia" in Plant Disease, 101, no. 12 (2017):2145-2146,
https://doi.org/10.1094/PDIS-05-17-0708-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Janjatović, Sanja
AU  - Budnar, M.
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/473
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia
EP  - 2146
IS  - 12
SP  - 2145
VL  - 101
DO  - 10.1094/PDIS-05-17-0708-PDN
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Milovanović, Predrag and Janjatović, Sanja and Budnar, M. and Dimkić, Ivica and Stanković, Slaviša",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia",
pages = "2146-2145",
number = "12",
volume = "101",
doi = "10.1094/PDIS-05-17-0708-PDN"
}

Popović, T., Jelušić, A., Milovanović, P., Janjatović, S., Budnar, M., Dimkić, I.,& Stanković, S.. (2017). First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(12), 2145-2146.
https://doi.org/10.1094/PDIS-05-17-0708-PDN

Popović T, Jelušić A, Milovanović P, Janjatović S, Budnar M, Dimkić I, Stanković S. First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia. in Plant Disease. 2017;101(12):2145-2146.
doi:10.1094/PDIS-05-17-0708-PDN .

Popović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Janjatović, Sanja, Budnar, M., Dimkić, Ivica, Stanković, Slaviša, "First Report of Pectobacterium atrosepticum, Causing Bacterial Soft Rot on Calla Lily in Serbia" in Plant Disease, 101, no. 12 (2017):2145-2146,
https://doi.org/10.1094/PDIS-05-17-0708-PDN . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Janse, Jaap
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Gavrilović, Veljko
AU  - Fira, Đorđe
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/396
AB  - The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques
EP  - 145
IS  - 1
SP  - 133
VL  - 141
DO  - 10.1007/s10658-014-0531-5
ER  - 

@article{
author = "Ivanović, Žarko and Popović, Tatjana and Janse, Jaap and Kojić, Milan and Stanković, Slaviša and Gavrilović, Veljko and Fira, Đorđe",
year = "2015",
abstract = "The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques",
pages = "145-133",
number = "1",
volume = "141",
doi = "10.1007/s10658-014-0531-5"
}

Ivanović, Ž., Popović, T., Janse, J., Kojić, M., Stanković, S., Gavrilović, V.,& Fira, Đ.. (2015). Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology
Springer, Dordrecht., 141(1), 133-145.
https://doi.org/10.1007/s10658-014-0531-5

Ivanović Ž, Popović T, Janse J, Kojić M, Stanković S, Gavrilović V, Fira Đ. Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology. 2015;141(1):133-145.
doi:10.1007/s10658-014-0531-5 .

Ivanović, Žarko, Popović, Tatjana, Janse, Jaap, Kojić, Milan, Stanković, Slaviša, Gavrilović, Veljko, Fira, Đorđe, "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques" in European Journal of Plant Pathology, 141, no. 1 (2015):133-145,
https://doi.org/10.1007/s10658-014-0531-5 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Stanković, Slaviša
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/345
AB  - A diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobacco and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - A method for the rapid detection and identification of halo blight pathogen on common bean
EP  - 1399
IS  - 4
SP  - 1393
VL  - 66
DO  - 10.2298/ABS1404393P
ER  - 

@article{
author = "Popović, Tatjana and Balaž, Jelica and Stanković, Slaviša",
year = "2014",
abstract = "A diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobacco and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "A method for the rapid detection and identification of halo blight pathogen on common bean",
pages = "1399-1393",
number = "4",
volume = "66",
doi = "10.2298/ABS1404393P"
}

Popović, T., Balaž, J.,& Stanković, S.. (2014). A method for the rapid detection and identification of halo blight pathogen on common bean. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 66(4), 1393-1399.
https://doi.org/10.2298/ABS1404393P

Popović T, Balaž J, Stanković S. A method for the rapid detection and identification of halo blight pathogen on common bean. in Archives of Biological Sciences. 2014;66(4):1393-1399.
doi:10.2298/ABS1404393P .

Popović, Tatjana, Balaž, Jelica, Stanković, Slaviša, "A method for the rapid detection and identification of halo blight pathogen on common bean" in Archives of Biological Sciences, 66, no. 4 (2014):1393-1399,
https://doi.org/10.2298/ABS1404393P . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Milovanović, Predrag
AU  - Dolovac, Nenad
AU  - Poštić, Dobrivoj
AU  - Stanković, Slaviša
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/279
AB  - Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli
EP  - 593
IS  - 2
SP  - 585
VL  - 65
DO  - 10.2298/ABS1302585P
ER  - 

@article{
author = "Popović, Tatjana and Jošić, Dragana and Starović, Mira and Milovanović, Predrag and Dolovac, Nenad and Poštić, Dobrivoj and Stanković, Slaviša",
year = "2013",
abstract = "Thirty-six strains of Xanthomonas campestris pv. campestris (Xcc) isolated from cabbage, kale and broccoli were identified according to their pathogenicity, phenotypic and genotypic characterization. Pathogenicity was confirmed by the injection method with a hypodermic syringe into the mesophilic tissue of cabbage leaves. All strains were Gramnegative, aerobic, catalase-positive, oxidase-negative, grew at 35°C, produced levan, H2S and indole, did not reduce nitrate, hydrolyzed Tween 80, starch, gelatin and esculin and did not show tolerance to 0.1 and 0.02% TTC. The strains produced acid from d-arabinose, arginine, dulcitol, galactose, d-glucose, maltose, mannose, sorbitol, sucrose and xylose. The genetic characterization was based on the sequence analyses of 16S rDNA and ERIC and BOX PCR. Strains of different pathovars were also used to compare PCR resulting patterns. BOX-PCR of the strains from kale and broccoli, obtained using (GTG)5 primer, yielded patterns with a high similarity level to pathovar reference strain Xcc. The strains from cabbage yielded BOX and ERIC product patterns, distinguishing them from the other tested strains and reference strains. 16S rDNA of the representative strains was closely related to Xcc strain ATCC 33913. ERIC PCR and BOX using (GTG)5 primer generated different Xcc patterns and were effective in distinguishing strains from different plant hosts.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli",
pages = "593-585",
number = "2",
volume = "65",
doi = "10.2298/ABS1302585P"
}

Popović, T., Jošić, D., Starović, M., Milovanović, P., Dolovac, N., Poštić, D.,& Stanković, S.. (2013). Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 65(2), 585-593.
https://doi.org/10.2298/ABS1302585P

Popović T, Jošić D, Starović M, Milovanović P, Dolovac N, Poštić D, Stanković S. Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli. in Archives of Biological Sciences. 2013;65(2):585-593.
doi:10.2298/ABS1302585P .

Popović, Tatjana, Jošić, Dragana, Starović, Mira, Milovanović, Predrag, Dolovac, Nenad, Poštić, Dobrivoj, Stanković, Slaviša, "Phenotypic and genotypic characterization of Xanthomonas campestris strains isolated from cabbage, kale and broccoli" in Archives of Biological Sciences, 65, no. 2 (2013):585-593,
https://doi.org/10.2298/ABS1302585P . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
AU  - Stanković, Slaviša
AU  - Berić, Tanja
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/251
AB  - During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.
AB  - Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia
T1  - Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji
EP  - 961
IS  - 3
SP  - 953
VL  - 45
DO  - 10.2298/GENSR1303953G
ER  - 

@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana and Stanković, Slaviša and Berić, Tanja and Fira, Đorđe",
year = "2013",
abstract = "During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia., Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia, Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji",
pages = "961-953",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303953G"
}

Gavrilović, V., Ivanović, Ž., Popović, T., Živković, S., Stanković, S., Berić, T.,& Fira, Đ.. (2013). Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 45(3), 953-961.
https://doi.org/10.2298/GENSR1303953G

Gavrilović V, Ivanović Ž, Popović T, Živković S, Stanković S, Berić T, Fira Đ. Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade. 2013;45(3):953-961.
doi:10.2298/GENSR1303953G .

Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia" in Genetika-Belgrade, 45, no. 3 (2013):953-961,
https://doi.org/10.2298/GENSR1303953G . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Živković, Svetlana
AU  - Berić, Tanja
AU  - Ivanović, Žarko
AU  - Gavrilović, Veljko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/291
AB  - Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi
EP  - 321
IS  - 3
SP  - 312
VL  - 65
DO  - 10.1016/j.biocontrol.2013.03.012
ER  - 

@article{
author = "Dimkić, Ivica and Živković, Svetlana and Berić, Tanja and Ivanović, Žarko and Gavrilović, Veljko and Stanković, Slaviša and Fira, Đorđe",
year = "2013",
abstract = "Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi",
pages = "321-312",
number = "3",
volume = "65",
doi = "10.1016/j.biocontrol.2013.03.012"
}

Dimkić, I., Živković, S., Berić, T., Ivanović, Ž., Gavrilović, V., Stanković, S.,& Fira, Đ.. (2013). Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 65(3), 312-321.
https://doi.org/10.1016/j.biocontrol.2013.03.012

Dimkić I, Živković S, Berić T, Ivanović Ž, Gavrilović V, Stanković S, Fira Đ. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control. 2013;65(3):312-321.
doi:10.1016/j.biocontrol.2013.03.012 .

Dimkić, Ivica, Živković, Svetlana, Berić, Tanja, Ivanović, Žarko, Gavrilović, Veljko, Stanković, Slaviša, Fira, Đorđe, "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi" in Biological Control, 65, no. 3 (2013):312-321,
https://doi.org/10.1016/j.biocontrol.2013.03.012 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Stanković, Slaviša
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Kojić, Milan
AU  - Fira, Đorđe
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/208
AB  - Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia
EP  - 203
IS  - 1
SP  - 191
VL  - 134
DO  - 10.1007/s10658-012-9978-4
ER  - 

@article{
author = "Ivanović, Žarko and Stanković, Slaviša and Živković, Svetlana and Gavrilović, Veljko and Kojić, Milan and Fira, Đorđe",
year = "2012",
abstract = "Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia",
pages = "203-191",
number = "1",
volume = "134",
doi = "10.1007/s10658-012-9978-4"
}

Ivanović, Ž., Stanković, S., Živković, S., Gavrilović, V., Kojić, M.,& Fira, Đ.. (2012). Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 134(1), 191-203.
https://doi.org/10.1007/s10658-012-9978-4

Ivanović Ž, Stanković S, Živković S, Gavrilović V, Kojić M, Fira Đ. Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology. 2012;134(1):191-203.
doi:10.1007/s10658-012-9978-4 .

Ivanović, Žarko, Stanković, Slaviša, Živković, Svetlana, Gavrilović, Veljko, Kojić, Milan, Fira, Đorđe, "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia" in European Journal of Plant Pathology, 134, no. 1 (2012):191-203,
https://doi.org/10.1007/s10658-012-9978-4 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Starović, Mira
AU  - Jošić, Dragana
AU  - Stanković, Slaviša
AU  - Gavrilović, Veljko
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/115
AB  - Pseudomonas syringae is a widespread and economically important plant pathogen, one found on a number of hosts, including fruit trees, field crops, vegetables, and ornamental plants. This bacterium has been experimentally identified as a parasite of pear, apple, apricot, peach, cherry, sour cherry, plum, and raspberry. The present study was designed to establish differences between strains isolated from fruit trees in Serbia. The pathogenic and biochemical characteristics of isolates were studied. The BOX-PCR method was used to generate genomic fingerprints of Pseudomonas syringae isolates and to identify strains that were previously not distinguishable by other classification methods. Different Bacillus sp. strains were tested for in vitro inhibitory activity against Pseudononas syringae isolates. Bacillus sp. strains show inhibitory activity only against P. syringae isolates that originated from peach. The obtained results demonstrate that the population of the bacterium Pseudomonas syringae from the fruit trees in Serbia is very diverse.
AB  - Pseudomonas syringae je široko rasprostranjena i ekonomski značajna fitopatogena bakterija, sa širokim krugom domaćina koji uključuje voćke, ratarske, povrtarske i ukrasne biljke. Pseudomonas syringae u Srbiji je eksperimentalno potvrđen kao parazit kruške, jabuke, kajsije, breskve, trešnje, višnje, šljive i maline. Cilj ove studije bio je da utvrdi postojanje eventualnih razlika između sojeva izolovanih sa različitih vrsta voćaka u Srbiji. Proučavane su patogene i biohemijske osobine sojeva. BOX-PCR je korišćen za dobijanje profila izolata Pseudomonas syringae u cilju identifikacije sojeva koji se ne mogu utvrditi drugim metodama. Različiti sojevi roda Bacillus su testirani u cilju utvrđivanja njihove in vitro inhibitorne aktivnosti. Sojevi roda Bacillus su pokazali inhibitornu aktivnost samo na P. syringae izolovanih sa breskve. Dobijeni rezultati pokazali suda je populacija bakterije Pseudomonas syringae poreklom sa voća u Srbiji vrlo raznovrsna.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia
T1  - Proučavanje raznovrsnosti bakterije Pseudomonas syringae poreklom sa različitih voćaka u Srbiji
EP  - 870
IS  - 4
SP  - 863
VL  - 61
DO  - 10.2298/ABS0904863I
ER  - 

@article{
author = "Ivanović, Žarko and Živković, Svetlana and Starović, Mira and Jošić, Dragana and Stanković, Slaviša and Gavrilović, Veljko",
year = "2009",
abstract = "Pseudomonas syringae is a widespread and economically important plant pathogen, one found on a number of hosts, including fruit trees, field crops, vegetables, and ornamental plants. This bacterium has been experimentally identified as a parasite of pear, apple, apricot, peach, cherry, sour cherry, plum, and raspberry. The present study was designed to establish differences between strains isolated from fruit trees in Serbia. The pathogenic and biochemical characteristics of isolates were studied. The BOX-PCR method was used to generate genomic fingerprints of Pseudomonas syringae isolates and to identify strains that were previously not distinguishable by other classification methods. Different Bacillus sp. strains were tested for in vitro inhibitory activity against Pseudononas syringae isolates. Bacillus sp. strains show inhibitory activity only against P. syringae isolates that originated from peach. The obtained results demonstrate that the population of the bacterium Pseudomonas syringae from the fruit trees in Serbia is very diverse., Pseudomonas syringae je široko rasprostranjena i ekonomski značajna fitopatogena bakterija, sa širokim krugom domaćina koji uključuje voćke, ratarske, povrtarske i ukrasne biljke. Pseudomonas syringae u Srbiji je eksperimentalno potvrđen kao parazit kruške, jabuke, kajsije, breskve, trešnje, višnje, šljive i maline. Cilj ove studije bio je da utvrdi postojanje eventualnih razlika između sojeva izolovanih sa različitih vrsta voćaka u Srbiji. Proučavane su patogene i biohemijske osobine sojeva. BOX-PCR je korišćen za dobijanje profila izolata Pseudomonas syringae u cilju identifikacije sojeva koji se ne mogu utvrditi drugim metodama. Različiti sojevi roda Bacillus su testirani u cilju utvrđivanja njihove in vitro inhibitorne aktivnosti. Sojevi roda Bacillus su pokazali inhibitornu aktivnost samo na P. syringae izolovanih sa breskve. Dobijeni rezultati pokazali suda je populacija bakterije Pseudomonas syringae poreklom sa voća u Srbiji vrlo raznovrsna.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia, Proučavanje raznovrsnosti bakterije Pseudomonas syringae poreklom sa različitih voćaka u Srbiji",
pages = "870-863",
number = "4",
volume = "61",
doi = "10.2298/ABS0904863I"
}

Ivanović, Ž., Živković, S., Starović, M., Jošić, D., Stanković, S.,& Gavrilović, V.. (2009). Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 61(4), 863-870.
https://doi.org/10.2298/ABS0904863I

Ivanović Ž, Živković S, Starović M, Jošić D, Stanković S, Gavrilović V. Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia. in Archives of Biological Sciences. 2009;61(4):863-870.
doi:10.2298/ABS0904863I .

Ivanović, Žarko, Živković, Svetlana, Starović, Mira, Jošić, Dragana, Stanković, Slaviša, Gavrilović, Veljko, "Diversity among Pseudomonas syringae strains originating from fruit trees in Serbia" in Archives of Biological Sciences, 61, no. 4 (2009):863-870,
https://doi.org/10.2298/ABS0904863I . .