Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease
American Phytopathological Society..
https://doi.org/10.1094/PDIS-05-22-1019-PDN

Živković S, Trkulja N, Kovačević S, Stošić S. First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease. 2023;.
doi:10.1094/PDIS-05-22-1019-PDN .

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia" in Plant Disease (2023),
https://doi.org/10.1094/PDIS-05-22-1019-PDN . .

TY  - CONF
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Kovačević, Stefan
AU  - Stošić, Stefan
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1202
AB  - Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške
EP  - 45
SP  - 44
ER  - 

@conference{
author = "Živković, Svetlana and Trkulja, Nenad and Kovačević, Stefan and Stošić, Stefan",
year = "2023",
abstract = "Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške",
pages = "45-44"
}

Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 44-45.

Živković S, Trkulja N, Kovačević S, Stošić S. Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:44-45..

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):44-45.

TY  - CONF
AU  - Starović, Mira
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Stošić, Stefan
AU  - Živković, Svetlana
AU  - Aleksic, Goran
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/787
AB  - Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.
PB  - University of East Sarajevo
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
T1  - Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic
EP  - 650
IS  - 645
ER  - 

@conference{
author = "Starović, Mira and Ristić, Lela and Vučurović, Ivan and Blagojević, Jovana and Stošić, Stefan and Živković, Svetlana and Aleksic, Goran",
year = "2022",
abstract = "Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.",
publisher = "University of East Sarajevo",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022",
title = "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic",
pages = "650",
number = "645"
}

Starović, M., Ristić, L., Vučurović, I., Blagojević, J., Stošić, S., Živković, S.,& Aleksic, G.. (2022). Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
University of East Sarajevo.(645).

Starović M, Ristić L, Vučurović I, Blagojević J, Stošić S, Živković S, Aleksic G. Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022. 2022;(645):null-650..

Starović, Mira, Ristić, Lela, Vučurović, Ivan, Blagojević, Jovana, Stošić, Stefan, Živković, Svetlana, Aleksic, Goran, "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022, no. 645 (2022).

TY  - JOUR
AU  - Stošić, Stefan
AU  - Delić, Dušica
AU  - Živković, Svetlana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/627
AB  - Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Polyphasic identification of decay agents of lemon fruits in Serbia
SP  - 73
SP  - 87
VL  - 143
DO  - 10.2298/ZMSPN2243073S
ER  - 

@article{
author = "Stošić, Stefan and Delić, Dušica and Živković, Svetlana",
year = "2022",
abstract = "Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Polyphasic identification of decay agents of lemon fruits in Serbia",
pages = "73-87",
volume = "143",
doi = "10.2298/ZMSPN2243073S"
}

Stošić, S., Delić, D.,& Živković, S.. (2022). Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 143, 73.
https://doi.org/10.2298/ZMSPN2243073S

Stošić S, Delić D, Živković S. Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;143:73.
doi:10.2298/ZMSPN2243073S .

Stošić, Stefan, Delić, Dušica, Živković, Svetlana, "Polyphasic identification of decay agents of lemon fruits in Serbia" in Zbornik Matice srpske za prirodne nauke, 143 (2022):73,
https://doi.org/10.2298/ZMSPN2243073S . .

TY  - JOUR
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Vučurović, Ana
AU  - Živković, Svetlana
AU  - Gašić, Katarina
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/716
AB  - The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.
PB  - Springer Verlag
T2  - European Journal of Plant Pathology
T1  - Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia
EP  - 324
IS  - 2
SP  - 315
VL  - 160
DO  - 10.1007/s10658-021-02243-6
ER  - 

@article{
author = "Ristić, Lela and Vučurović, Ivan and Vučurović, Ana and Živković, Svetlana and Gašić, Katarina and Kuzmanović, Slobodan and Starović, Mira",
year = "2021",
abstract = "The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.",
publisher = "Springer Verlag",
journal = "European Journal of Plant Pathology",
title = "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia",
pages = "324-315",
number = "2",
volume = "160",
doi = "10.1007/s10658-021-02243-6"
}

Ristić, L., Vučurović, I., Vučurović, A., Živković, S., Gašić, K., Kuzmanović, S.,& Starović, M.. (2021). Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology
Springer Verlag., 160(2), 315-324.
https://doi.org/10.1007/s10658-021-02243-6

Ristić L, Vučurović I, Vučurović A, Živković S, Gašić K, Kuzmanović S, Starović M. Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology. 2021;160(2):315-324.
doi:10.1007/s10658-021-02243-6 .

Ristić, Lela, Vučurović, Ivan, Vučurović, Ana, Živković, Svetlana, Gašić, Katarina, Kuzmanović, Slobodan, Starović, Mira, "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia" in European Journal of Plant Pathology, 160, no. 2 (2021):315-324,
https://doi.org/10.1007/s10658-021-02243-6 . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Savković, Željko
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/726
AB  - Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia
EP  - 3521
IS  - 11
SP  - 3510
VL  - 105
DO  - 10.1094/PDIS-01-21-0037-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Savković, Željko and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2021",
abstract = "Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia",
pages = "3521-3510",
number = "11",
volume = "105",
doi = "10.1094/PDIS-01-21-0037-RE"
}

Stošić, S., Ristić, L., Savković, Ž., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2021). Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease
American Phytopathological Society., 105(11), 3510-3521.
https://doi.org/10.1094/PDIS-01-21-0037-RE

Stošić S, Ristić L, Savković Ž, Ljaljevic Grbic M, Vukojevic J, Živković S. Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease. 2021;105(11):3510-3521.
doi:10.1094/PDIS-01-21-0037-RE .

Stošić, Stefan, Ristić, Lela, Savković, Željko, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia" in Plant Disease, 105, no. 11 (2021):3510-3521,
https://doi.org/10.1094/PDIS-01-21-0037-RE . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Gašić, Katarina
AU  - Starović, Mira
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/717
AB  - Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia
EP  - 667
IS  - 3
SP  - 656
VL  - 104
DO  - 10.1094/PDIS-08-19-1806-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Gašić, Katarina and Starović, Mira and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2020",
abstract = "Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia",
pages = "667-656",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1806-RE"
}

Stošić, S., Ristić, L., Gašić, K., Starović, M., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2020). Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease
The American Phytopathological Society., 104(3), 656-667.
https://doi.org/10.1094/PDIS-08-19-1806-RE

Stošić S, Ristić L, Gašić K, Starović M, Ljaljevic Grbic M, Vukojevic J, Živković S. Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease. 2020;104(3):656-667.
doi:10.1094/PDIS-08-19-1806-RE .

Stošić, Stefan, Ristić, Lela, Gašić, Katarina, Starović, Mira, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia" in Plant Disease, 104, no. 3 (2020):656-667,
https://doi.org/10.1094/PDIS-08-19-1806-RE . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

TY  - JOUR
AU  - Aleksić, Goran
AU  - Milićević, Zoran
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Delibašić, Goran
AU  - Živković, Svetlana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5229
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/599
AB  - The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection.
AB  - Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Efficacy of copper citrate in grapevine disease control
T1  - Efikasnost bakar-citrata u zaštiti vinove loze od bolesti
EP  - 109
IS  - 2
SP  - 103
VL  - 34
ER  - 

@article{
author = "Aleksić, Goran and Milićević, Zoran and Kuzmanović, Slobodan and Starović, Mira and Stevanović, Miloš and Delibašić, Goran and Živković, Svetlana",
year = "2019",
abstract = "The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection., Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Efficacy of copper citrate in grapevine disease control, Efikasnost bakar-citrata u zaštiti vinove loze od bolesti",
pages = "109-103",
number = "2",
volume = "34"
}

Aleksić, G., Milićević, Z., Kuzmanović, S., Starović, M., Stevanović, M., Delibašić, G.,& Živković, S.. (2019). Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(2), 103-109.

Aleksić G, Milićević Z, Kuzmanović S, Starović M, Stevanović M, Delibašić G, Živković S. Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina. 2019;34(2):103-109..

Aleksić, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Stevanović, Miloš, Delibašić, Goran, Živković, Svetlana, "Efficacy of copper citrate in grapevine disease control" in Pesticidi i fitomedicina, 34, no. 2 (2019):103-109.

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksic, Goran
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1015
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Primena bakar citrata pri defolijaciji sadnica višnje i breskve
EP  - 49
IS  - 5
SP  - 41
VL  - 24
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksic, Goran and Živković, Svetlana and Stošić, Stefan and Starović, Mira and Kuzmanović, Slobodan",
year = "2018",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Primena bakar citrata pri defolijaciji sadnica višnje i breskve",
pages = "49-41",
number = "5",
volume = "24"
}

Gavrilović, V., Milićević, Z., Aleksic, G., Živković, S., Stošić, S., Starović, M.,& Kuzmanović, S.. (2018). Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 24(5), 41-49.

Gavrilović V, Milićević Z, Aleksic G, Živković S, Stošić S, Starović M, Kuzmanović S. Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik. 2018;24(5):41-49..

Gavrilović, Veljko, Milićević, Zoran, Aleksic, Goran, Živković, Svetlana, Stošić, Stefan, Starović, Mira, Kuzmanović, Slobodan, "Primena bakar citrata pri defolijaciji sadnica višnje i breskve" in Zbornik Instituta PKB agroekonomik, 24, no. 5 (2018):41-49.

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 

@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}

Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z

Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .

Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/495
AB  - The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks.
AB  - U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Effects of copper citrate as a defoliant in nursery fruit stock production
T1  - Bakar citrat - defolijant u proizvodnji sadnica voćaka
EP  - 236
IS  - 3-4
SP  - 231
VL  - 32
DO  - 10.2298/PIF1704231G
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksić, Goran and Starović, Mira and Živković, Svetlana and Stošić, Stefan and Kuzmanović, Slobodan",
year = "2017",
abstract = "The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks., U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Effects of copper citrate as a defoliant in nursery fruit stock production, Bakar citrat - defolijant u proizvodnji sadnica voćaka",
pages = "236-231",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704231G"
}

Gavrilović, V., Milićević, Z., Aleksić, G., Starović, M., Živković, S., Stošić, S.,& Kuzmanović, S.. (2017). Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 231-236.
https://doi.org/10.2298/PIF1704231G

Gavrilović V, Milićević Z, Aleksić G, Starović M, Živković S, Stošić S, Kuzmanović S. Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina. 2017;32(3-4):231-236.
doi:10.2298/PIF1704231G .

Gavrilović, Veljko, Milićević, Zoran, Aleksić, Goran, Starović, Mira, Živković, Svetlana, Stošić, Stefan, Kuzmanović, Slobodan, "Effects of copper citrate as a defoliant in nursery fruit stock production" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):231-236,
https://doi.org/10.2298/PIF1704231G . .

TY  - JOUR
AU  - Milisavljević, M.
AU  - Živković, Svetlana
AU  - Pekmezović, M.
AU  - Stanković, N.
AU  - Vojnović, S.
AU  - Vasiljević, B.
AU  - Senerović, L.
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/378
AB  - AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.
PB  - Wiley, Hoboken
T2  - Journal of Applied Microbiology
T1  - Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin
EP  - 1434
IS  - 6
SP  - 1426
VL  - 118
DO  - 10.1111/jam.12811
ER  - 

@article{
author = "Milisavljević, M. and Živković, Svetlana and Pekmezović, M. and Stanković, N. and Vojnović, S. and Vasiljević, B. and Senerović, L.",
year = "2015",
abstract = "AimsThe aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (DDHR) on a wide range of fungal pathogens and its potential to control plant fungal diseases. Methods and ResultsThe antifungal activity of DDHR invitro was examined against common human and plant pathogenic fungi using a broth microdilution assay and a disk diffusion assay. Minimum inhibitory concentrations ranged from 125 to 35gml(-1). A radial growth inhibition assay showed that DDHR inhibited mycelia growth, inducing mycelial necrosis and affecting sporulation. During the invivo assay on apple fruits administration of DDHR 1h before fungal inoculation inhibited spreading of the infection. Importantly, DDHR exhibited no phytotoxic effects on the model plant, Capsicum annum, verified by the plant growth rate and chlorophyll content. ConclusionsDDHR inhibits growth of various plant pathogens invitro with the strongest activity against Alternaria alternata, Colletotrichum acutatum and Penicillium expansum, and protects apple fruits from decay. Significance and Impact of the StudyThis is the first report of the inhibitory effect of DDHR on important pathogenic fungal isolates. DDHR could be a good scaffold for developing new antifungal agents for fruit and vegetable protection.",
publisher = "Wiley, Hoboken",
journal = "Journal of Applied Microbiology",
title = "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin",
pages = "1434-1426",
number = "6",
volume = "118",
doi = "10.1111/jam.12811"
}

Milisavljević, M., Živković, S., Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B.,& Senerović, L.. (2015). Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology
Wiley, Hoboken., 118(6), 1426-1434.
https://doi.org/10.1111/jam.12811

Milisavljević M, Živković S, Pekmezović M, Stanković N, Vojnović S, Vasiljević B, Senerović L. Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin. in Journal of Applied Microbiology. 2015;118(6):1426-1434.
doi:10.1111/jam.12811 .

Milisavljević, M., Živković, Svetlana, Pekmezović, M., Stanković, N., Vojnović, S., Vasiljević, B., Senerović, L., "Control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin" in Journal of Applied Microbiology, 118, no. 6 (2015):1426-1434,
https://doi.org/10.1111/jam.12811 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Živković, Svetlana
AU  - Popović, Tatjana
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/397
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.
EP  - 1036
IS  - 7
SP  - 1035
VL  - 99
DO  - 10.1094/PDIS-10-14-1096-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Živković, Svetlana and Popović, Tatjana and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.",
pages = "1036-1035",
number = "7",
volume = "99",
doi = "10.1094/PDIS-10-14-1096-PDN"
}

Trkulja, N., Milosavljević, A., Živković, S., Popović, T., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(7), 1035-1036.
https://doi.org/10.1094/PDIS-10-14-1096-PDN

Trkulja N, Milosavljević A, Živković S, Popović T, Mitrović M, Jović J, Toševski I. First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease. 2015;99(7):1035-1036.
doi:10.1094/PDIS-10-14-1096-PDN .

Trkulja, Nenad, Milosavljević, Anja, Živković, Svetlana, Popović, Tatjana, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia." in Plant Disease, 99, no. 7 (2015):1035-1036,
https://doi.org/10.1094/PDIS-10-14-1096-PDN . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/362
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stojanović, Saša
AU  - Balaž, Jelica
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/305
AB  - In the morphological studies were included isolates of Colletotrichum spp. originating from pear, apple and sour cherry fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97). The colony characteristics, appearance, shape and size of conidioma, appressoria, and conidia of isolates of Colletotrichum spp. were studied. The four morphological groups of colonies on PDA were observed, confirming the great phenotypic variability of isolates of Colletotrichum spp. Most isolates from apple and pear fruits (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9), formed the first morphological group. The isolates from sour cherry (VC-3, VC-5, VC-7, VC-9) are separated in the fourth morphological group. The size and shape of conidia from these isolates fit the description of C. gloe­osporioides. The chromogenic isolates from pear (KC-21, KC-23, KC-82) formed the second morphological group, and in the third morphological group is isolate (JC-4) from apple fruit. The morphology of conidia from these isolates fit the description of C. acutatum. During this research were evident that all isolates formed conidioma - acervuli. The tested cultures of Colletotrichum spp. did not form teleomorpf. Because of outstanding variability, most of the studied characteristics cannot be independently applied as taxonomic criteria. Obtai­ned results showed that, for Colletotrichum species classification, at least two taxonomic criteria should be used: morphological features combined with molecular identification.
AB  - U morfološka ispitivanja su uključeni izolati Colletotrichum spp. poreklom sa plodova kruške, jabuke i višnje, kao i referentni sojevi C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). Proučavanjem njihovih morfoloških karakteristika, dobijeni su podaci o izgledu ispitivanih kolonija, odlikama plodonosnog tela, apresorija, veličini i obliku konidija. Na podlozi KDA moguće je razlikovati četiri morfološke grupe kolonija, što potvrđu­je fenotipsku varijabilnost proučavanih izolata Colletotrichum spp. Većina izolata sa ploda kruške i jabuke (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9) obrazuje prvu morfološku grupu. Izolati sa ploda višnje (VC-3, VC-5, VC-7, VC-9), su izdvojeni u četvrtu morfološku grupu. Veličina i oblik konidija ovih izolata odgovara opisu vrste C. gloeosporioides. Hromogeni izolati sa kruške (KC-21, KC-23, KC-82), obrazuju drugu morfološku grupu, a u trećoj morfološkoj grupi je izolat sa ploda jabuke (JC-4). Morfologija konidija ovih izolata odgovara opisu vrste C. acutatum. Tokom ovih istraživanja je utvrđeno da svi izolati obrazuju plodonosna tela - acervule. Testirane kulture Colletotrichum spp. ne formiraju teleomorfni stadijum. Zbog izražene varijabilnosti, većina ispitanih osobina ne mogu se primeni kao samostalni taksonomski kriterijum. Na osnovu dobijenih rezultata, za klasifikaciju vrsta roda Colletotrichum, potrebno je koristiti najmanje dva taksonomska kriterijuma: morfološke karakteristike uporedo sa molekularnom identifikacijom.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose
T1  - Morfološke karakteristike izolata Colletotrichum spp. - prouzrokovača antraknoze
EP  - 136
IS  - 3
SP  - 124
VL  - 65
DO  - 10.5937/zasbilj1403124Z
ER  - 

@article{
author = "Živković, Svetlana and Stojanović, Saša and Balaž, Jelica",
year = "2014",
abstract = "In the morphological studies were included isolates of Colletotrichum spp. originating from pear, apple and sour cherry fruits, as well as reference strains of C. acutatum (CBS 294.67) and C. gloeosporioides (CBS 516.97). The colony characteristics, appearance, shape and size of conidioma, appressoria, and conidia of isolates of Colletotrichum spp. were studied. The four morphological groups of colonies on PDA were observed, confirming the great phenotypic variability of isolates of Colletotrichum spp. Most isolates from apple and pear fruits (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9), formed the first morphological group. The isolates from sour cherry (VC-3, VC-5, VC-7, VC-9) are separated in the fourth morphological group. The size and shape of conidia from these isolates fit the description of C. gloe­osporioides. The chromogenic isolates from pear (KC-21, KC-23, KC-82) formed the second morphological group, and in the third morphological group is isolate (JC-4) from apple fruit. The morphology of conidia from these isolates fit the description of C. acutatum. During this research were evident that all isolates formed conidioma - acervuli. The tested cultures of Colletotrichum spp. did not form teleomorpf. Because of outstanding variability, most of the studied characteristics cannot be independently applied as taxonomic criteria. Obtai­ned results showed that, for Colletotrichum species classification, at least two taxonomic criteria should be used: morphological features combined with molecular identification., U morfološka ispitivanja su uključeni izolati Colletotrichum spp. poreklom sa plodova kruške, jabuke i višnje, kao i referentni sojevi C. acutatum (CBS 294.67) i C. gloeosporioides (CBS 516.97). Proučavanjem njihovih morfoloških karakteristika, dobijeni su podaci o izgledu ispitivanih kolonija, odlikama plodonosnog tela, apresorija, veličini i obliku konidija. Na podlozi KDA moguće je razlikovati četiri morfološke grupe kolonija, što potvrđu­je fenotipsku varijabilnost proučavanih izolata Colletotrichum spp. Većina izolata sa ploda kruške i jabuke (KC-6, KC-9, KC-12, JC-5, JC-7, JC-9) obrazuje prvu morfološku grupu. Izolati sa ploda višnje (VC-3, VC-5, VC-7, VC-9), su izdvojeni u četvrtu morfološku grupu. Veličina i oblik konidija ovih izolata odgovara opisu vrste C. gloeosporioides. Hromogeni izolati sa kruške (KC-21, KC-23, KC-82), obrazuju drugu morfološku grupu, a u trećoj morfološkoj grupi je izolat sa ploda jabuke (JC-4). Morfologija konidija ovih izolata odgovara opisu vrste C. acutatum. Tokom ovih istraživanja je utvrđeno da svi izolati obrazuju plodonosna tela - acervule. Testirane kulture Colletotrichum spp. ne formiraju teleomorfni stadijum. Zbog izražene varijabilnosti, većina ispitanih osobina ne mogu se primeni kao samostalni taksonomski kriterijum. Na osnovu dobijenih rezultata, za klasifikaciju vrsta roda Colletotrichum, potrebno je koristiti najmanje dva taksonomska kriterijuma: morfološke karakteristike uporedo sa molekularnom identifikacijom.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose, Morfološke karakteristike izolata Colletotrichum spp. - prouzrokovača antraknoze",
pages = "136-124",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403124Z"
}

Živković, S., Stojanović, S.,& Balaž, J.. (2014). Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 124-136.
https://doi.org/10.5937/zasbilj1403124Z

Živković S, Stojanović S, Balaž J. Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose. in Zaštita bilja. 2014;65(3):124-136.
doi:10.5937/zasbilj1403124Z .

Živković, Svetlana, Stojanović, Saša, Balaž, Jelica, "Morphological characteristics of isolates of Colletotrichum spp.: Causal agents of anthracnose" in Zaštita bilja, 65, no. 3 (2014):124-136,
https://doi.org/10.5937/zasbilj1403124Z . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 

@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}

Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S

Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .

Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/337
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia
EP  - 1272
IS  - 9
SP  - 1272
VL  - 98
DO  - 10.1094/PDIS-01-14-0052-PDN
ER  - 

@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Popović, Tatjana and Dolovac, Nenad and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia",
pages = "1272-1272",
number = "9",
volume = "98",
doi = "10.1094/PDIS-01-14-0052-PDN"
}

Živković, S., Gavrilović, V., Popović, T., Dolovac, N.,& Trkulja, N.. (2014). First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1272-1272.
https://doi.org/10.1094/PDIS-01-14-0052-PDN

Živković S, Gavrilović V, Popović T, Dolovac N, Trkulja N. First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease. 2014;98(9):1272-1272.
doi:10.1094/PDIS-01-14-0052-PDN .

Živković, Svetlana, Gavrilović, Veljko, Popović, Tatjana, Dolovac, Nenad, Trkulja, Nenad, "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia" in Plant Disease, 98, no. 9 (2014):1272-1272,
https://doi.org/10.1094/PDIS-01-14-0052-PDN . .

TY  - CONF
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/320
AB  - Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XIII International Workshop on Fire Blight
T1  - Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method
EP  - 172
SP  - 169
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.26
ER  - 

@conference{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana",
year = "2014",
abstract = "Characteristics of Erwinia amylovora strains isolated from quince trees (Cydonia oblonga) in different localities in Serbia are presented in this paper. In the period 2010-2012, severe occurrence of fire blight on quince was recorded in almost all localities in Serbia. First symptoms appeared after blooming period, but most expressed symptoms were recorded during intensive growth of shoots. Isolated strains elicted HR in tobacco, necrosis of pear seedlings and immature pear fruits ('Williams'). Strains were non-fluorescent, produce levan; oxidase negative, catalase positive; hydrolysis of gelatine positive; esculin and starch tests were negative; strains metabolise sucrose, sucrose, fructose and galactose. After amplification of total DNA with REP-PCR followed by agarose gel electrophoresis, the tested isolates originating from quince showed indistinguishable PCR patterns for all investigated native strains that were identical to European reference strains.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method",
pages = "172-169",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.26"
}

Gavrilović, V., Ivanović, Ž., Popović, T.,& Živković, S.. (2014). Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 169-172.
https://doi.org/10.17660/ActaHortic.2014.1056.26

Gavrilović V, Ivanović Ž, Popović T, Živković S. Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method. in XIII International Workshop on Fire Blight. 2014;1056:169-172.
doi:10.17660/ActaHortic.2014.1056.26 .

Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, "Characterization of Erwinia amylovora Strains Isolated from Quince Trees in Serbia Using REP-PCR Method" in XIII International Workshop on Fire Blight, 1056 (2014):169-172,
https://doi.org/10.17660/ActaHortic.2014.1056.26 . .

TY  - JOUR
AU  - Nikolić, Ivan
AU  - Ivanović, Žarko
AU  - Blagojević, Jovana
AU  - Živković, Svetlana
AU  - Popović, Tatjana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/259
AB  - Biological control is an innovative, cost effective and eco-friendly approach for control of many plant diseases. Bacillus sp. and Trichoderma sp. were known for its mycoparasitic and antagonistic mechanism in the control of wide range of phytopathogenic diseases in many types of crops. This research is based on the antibacterial activities of some Bacillus spp. and Trichoderma harzianum in relation to the 10 selected phytopathogenic bacteria in vitro study. The results indicated that among 52 strains of Bacillus spp. presented against phytopathogenic bacteria, only 6 of them showed inhibition but only against Xanthomonas genera. Other Bacillus spp. strains weren't effective against tested phytopathogenic bacteria. Results of testing of T. harzianum efficacy against the growth of selected phytopathogenic bacteria showed that this fungi could be effective to strains from genera Pseudomonas and Xanthomonas but for other tested genera was ineffective. In further work, all of this trial need to be supported by evaluation of antimicrobial activity in vivo.
AB  - Biološka kontrola predstavlja inovativni, isplativ, ekološki pristup za suzbijanje mnogih biljnih bolesti. Vrste roda Bacillus i Trichoderma su poznati po svom mikoparazitskom i antagonističkom mehanizmu suzbijanja širokog spektra fitopatogenih prouzrokovača bolesti kod brojnih poljoprivrednih kultura. U ovom radu proučavano je baktericidno delovanje nekih vrsta roda Bacillus, kao i gljive Trichoderma harzianum na 10 odabranih fitopatogenih bakterija u in vitro uslovima. Prema dobijenim rezultatima, od ukupno 52 testirana izolata iz roda Bacillus, samo 6 je inhibiralo porast fitopatogenih bakterija i to samo iz roda Xanthomonas. Rezultati ispitivanja efikasnosti gljive T. harzianum su ukazali na mogućnost delovanja ove gljive na bakterije iz roda Pseudomonas i Xanthomonas, ali ne i na druge rodove fitopatogenih bakterija testirane u ovom radu. Dalja istraživanja treba bazirati na proučavanjima antimikrobne aktivnosti testiranih agenasa prema fitopatogenim bakterijama u in vivo ogledima.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria
T1  - Baktericidno delovanje nekih Bacillus spp. i Trichoderma harzianum na fitopatogene bakterije
EP  - 197
IS  - 4
SP  - 189
VL  - 64
ER  - 

@article{
author = "Nikolić, Ivan and Ivanović, Žarko and Blagojević, Jovana and Živković, Svetlana and Popović, Tatjana",
year = "2013",
abstract = "Biological control is an innovative, cost effective and eco-friendly approach for control of many plant diseases. Bacillus sp. and Trichoderma sp. were known for its mycoparasitic and antagonistic mechanism in the control of wide range of phytopathogenic diseases in many types of crops. This research is based on the antibacterial activities of some Bacillus spp. and Trichoderma harzianum in relation to the 10 selected phytopathogenic bacteria in vitro study. The results indicated that among 52 strains of Bacillus spp. presented against phytopathogenic bacteria, only 6 of them showed inhibition but only against Xanthomonas genera. Other Bacillus spp. strains weren't effective against tested phytopathogenic bacteria. Results of testing of T. harzianum efficacy against the growth of selected phytopathogenic bacteria showed that this fungi could be effective to strains from genera Pseudomonas and Xanthomonas but for other tested genera was ineffective. In further work, all of this trial need to be supported by evaluation of antimicrobial activity in vivo., Biološka kontrola predstavlja inovativni, isplativ, ekološki pristup za suzbijanje mnogih biljnih bolesti. Vrste roda Bacillus i Trichoderma su poznati po svom mikoparazitskom i antagonističkom mehanizmu suzbijanja širokog spektra fitopatogenih prouzrokovača bolesti kod brojnih poljoprivrednih kultura. U ovom radu proučavano je baktericidno delovanje nekih vrsta roda Bacillus, kao i gljive Trichoderma harzianum na 10 odabranih fitopatogenih bakterija u in vitro uslovima. Prema dobijenim rezultatima, od ukupno 52 testirana izolata iz roda Bacillus, samo 6 je inhibiralo porast fitopatogenih bakterija i to samo iz roda Xanthomonas. Rezultati ispitivanja efikasnosti gljive T. harzianum su ukazali na mogućnost delovanja ove gljive na bakterije iz roda Pseudomonas i Xanthomonas, ali ne i na druge rodove fitopatogenih bakterija testirane u ovom radu. Dalja istraživanja treba bazirati na proučavanjima antimikrobne aktivnosti testiranih agenasa prema fitopatogenim bakterijama u in vivo ogledima.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria, Baktericidno delovanje nekih Bacillus spp. i Trichoderma harzianum na fitopatogene bakterije",
pages = "197-189",
number = "4",
volume = "64"
}

Nikolić, I., Ivanović, Ž., Blagojević, J., Živković, S.,& Popović, T.. (2013). Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(4), 189-197.

Nikolić I, Ivanović Ž, Blagojević J, Živković S, Popović T. Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria. in Zaštita bilja. 2013;64(4):189-197..

Nikolić, Ivan, Ivanović, Žarko, Blagojević, Jovana, Živković, Svetlana, Popović, Tatjana, "Antibacterial activities of some Bacillus spp. and Trichoderma harzianum against phytopathogenic bacteria" in Zaštita bilja, 64, no. 4 (2013):189-197.

TY  - JOUR
AU  - Delić, Dušica
AU  - Stajković-Srbinović, Olivera
AU  - Živković, Svetlana
AU  - Protić, Nada
AU  - Rasulić, Nataša
AU  - Kuzmanović, Đorđe
AU  - Simić, Aleksandar
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/267
AB  - In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa.
AB  - U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria
T1  - Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama
EP  - 42
IS  - 1
SP  - 35
VL  - 64
ER  - 

@article{
author = "Delić, Dušica and Stajković-Srbinović, Olivera and Živković, Svetlana and Protić, Nada and Rasulić, Nataša and Kuzmanović, Đorđe and Simić, Aleksandar",
year = "2013",
abstract = "In the greenhouse experiment, the possibility of alfalfa (Medicago sativa L.) growth promotion by inoculation of preceding barley (Hordeum vulgare L.), with plant growth promoting rhizobacteria (PGPR) was examined. The aim of experiment was to select the effective strains as biofertilizer applied in plant rotation. Effects of inoculation with two Azotobacter and two Pseudomonas strains as well as one Sinorhizobium, Enterobacter and Bacillus strain on shoot dry weight and total N content of alfalfa were determined. The results pointed out significant plant growth promotion abilities of strains A1, A2 and P1 which increased alfalfa shoot dry weight over untreated control Ø, by 41, 39 and 35 %, respectively. These three strains increased total N content of alfalfa plants by 34.92 - 40.45% in respect to control Ø. The presented study showed a significant positive influence of preceding barley inoculation with rhizobacteria alone and their mixture on shoot yield and total N content of alfalfa. Results indicated that strains of Azotobacter sp., Pseudomonas sp. and Enterobacter sp. alone can be investigated in further researches as potential agents of biofertilizer for plant growth promotion of alfalfa., U eksprimentu u sudovima ispitana je mogućnost poboljšanja rasta lucerke (Medicago sativa L.) pomoću inokulacije ječma (Hordeum vulgare L.) kao preduseva bakterijama koje poboljšavaju rast biljaka (PGPR). Cilj eksperimenta je bio odabiranje efikasnih sojeva koji bi se primenili u plodoredu u formi biološkog đubriva. U radu je korišćeno sedam sojeva koji pripadaju sledećim bakterijskim vrstama: Sinorhizobium meliloti, Bacillus megaterium, Enterobacter sp, Azotobacter sp, kao i vrsti Pseudomonas sp. Efekat inokulacije ječma primenjenim sojevima je određen na osnovu suve nadzemne mase (SDW) i sadržaja ukupnog azota (N) u biljnoj masi lucerke. Rezultati su ukazali na sposobnost nekih sojeva da poboljšaju rast biljaka. Značajno je povećan prinos lucerke u odnosu na kontrolu Ø (lucerka gajena posle neinokulisanog ječma kao preduseva) inokulacijom sojevima Azotobacter-a A1 (41%) i A2 (39%) i Pseudomonas-a P1 (35%). Sadržaj ukupnog N je bio u korelaciji sa vrednostima SDW. Rezultati su ukazali na uticaj inokulacije ječma kao preduseva na prinos lucerke i njen kvalitet kao i da sojevi Azotobacter sp., Pseudomonas sp. i Enterobacter sp. imaju PGPR potencijal što daje osnovu za dalja ispitivanja i mogućnost primene kao bio­đubriva.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria, Poboljšanje rasta lucerke, Medicago sativa L. - pomoću inokulacije preduseva rizosfernim bakterijama",
pages = "42-35",
number = "1",
volume = "64"
}

Delić, D., Stajković-Srbinović, O., Živković, S., Protić, N., Rasulić, N., Kuzmanović, Đ.,& Simić, A.. (2013). Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(1), 35-42.

Delić D, Stajković-Srbinović O, Živković S, Protić N, Rasulić N, Kuzmanović Đ, Simić A. Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria. in Zaštita bilja. 2013;64(1):35-42..

Delić, Dušica, Stajković-Srbinović, Olivera, Živković, Svetlana, Protić, Nada, Rasulić, Nataša, Kuzmanović, Đorđe, Simić, Aleksandar, "Growth promotion of alfalfa, Medicago sativa L.: By inoculation of a preceding crop with rhizobacteria" in Zaštita bilja, 64, no. 1 (2013):35-42.

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
AU  - Stanković, Slaviša
AU  - Berić, Tanja
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/251
AB  - During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.
AB  - Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia
T1  - Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji
EP  - 961
IS  - 3
SP  - 953
VL  - 45
DO  - 10.2298/GENSR1303953G
ER  - 

@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana and Stanković, Slaviša and Berić, Tanja and Fira, Đorđe",
year = "2013",
abstract = "During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia., Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia, Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji",
pages = "961-953",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303953G"
}

Gavrilović, V., Ivanović, Ž., Popović, T., Živković, S., Stanković, S., Berić, T.,& Fira, Đ.. (2013). Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 45(3), 953-961.
https://doi.org/10.2298/GENSR1303953G

Gavrilović V, Ivanović Ž, Popović T, Živković S, Stanković S, Berić T, Fira Đ. Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade. 2013;45(3):953-961.
doi:10.2298/GENSR1303953G .

Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia" in Genetika-Belgrade, 45, no. 3 (2013):953-961,
https://doi.org/10.2298/GENSR1303953G . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Živković, Svetlana
AU  - Berić, Tanja
AU  - Ivanović, Žarko
AU  - Gavrilović, Veljko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/291
AB  - Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi
EP  - 321
IS  - 3
SP  - 312
VL  - 65
DO  - 10.1016/j.biocontrol.2013.03.012
ER  - 

@article{
author = "Dimkić, Ivica and Živković, Svetlana and Berić, Tanja and Ivanović, Žarko and Gavrilović, Veljko and Stanković, Slaviša and Fira, Đorđe",
year = "2013",
abstract = "Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi",
pages = "321-312",
number = "3",
volume = "65",
doi = "10.1016/j.biocontrol.2013.03.012"
}

Dimkić, I., Živković, S., Berić, T., Ivanović, Ž., Gavrilović, V., Stanković, S.,& Fira, Đ.. (2013). Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 65(3), 312-321.
https://doi.org/10.1016/j.biocontrol.2013.03.012

Dimkić I, Živković S, Berić T, Ivanović Ž, Gavrilović V, Stanković S, Fira Đ. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control. 2013;65(3):312-321.
doi:10.1016/j.biocontrol.2013.03.012 .

Dimkić, Ivica, Živković, Svetlana, Berić, Tanja, Ivanović, Žarko, Gavrilović, Veljko, Stanković, Slaviša, Fira, Đorđe, "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi" in Biological Control, 65, no. 3 (2013):312-321,
https://doi.org/10.1016/j.biocontrol.2013.03.012 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Ignjatov, Maja
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/283
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.
EP  - 1504
IS  - 11
SP  - 1504
VL  - 97
DO  - 10.1094/PDIS-03-13-0267-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Živković, Svetlana and Trkulja, Nenad and Ignjatov, Maja",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.",
pages = "1504-1504",
number = "11",
volume = "97",
doi = "10.1094/PDIS-03-13-0267-PDN"
}

Popović, T., Ivanović, Ž., Živković, S., Trkulja, N.,& Ignjatov, M.. (2013). First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(11), 1504-1504.
https://doi.org/10.1094/PDIS-03-13-0267-PDN

Popović T, Ivanović Ž, Živković S, Trkulja N, Ignjatov M. First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease. 2013;97(11):1504-1504.
doi:10.1094/PDIS-03-13-0267-PDN .

Popović, Tatjana, Ivanović, Žarko, Živković, Svetlana, Trkulja, Nenad, Ignjatov, Maja, "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia." in Plant Disease, 97, no. 11 (2013):1504-1504,
https://doi.org/10.1094/PDIS-03-13-0267-PDN . .