TY  - JOUR
AU  - Milićević, Zoran
AU  - Krnjajić, Slobodan
AU  - Stević, Milan
AU  - Ćirković, Jovana
AU  - Jelušić, Aleksandra
AU  - Pucarević, Mira
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/738
AB  - In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite- F-CSZ, nature zeolite- F-CNZ and gelatin- F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.
PB  - MDPI
T2  - Agriculture
T1  - Encapsulated clove bud essential oil: A new perspective as an eco-friendly biopesticide
IS  - 3
SP  - 338
VL  - 12
DO  - 10.3390/agriculture12030338
ER  - 

@article{
author = "Milićević, Zoran and Krnjajić, Slobodan and Stević, Milan and Ćirković, Jovana and Jelušić, Aleksandra and Pucarević, Mira and Popović Milovanović, Tatjana",
year = "2022",
abstract = "In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite- F-CSZ, nature zeolite- F-CNZ and gelatin- F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.",
publisher = "MDPI",
journal = "Agriculture",
title = "Encapsulated clove bud essential oil: A new perspective as an eco-friendly biopesticide",
number = "3",
pages = "338",
volume = "12",
doi = "10.3390/agriculture12030338"
}

Milićević, Z., Krnjajić, S., Stević, M., Ćirković, J., Jelušić, A., Pucarević, M.,& Popović Milovanović, T.. (2022). Encapsulated clove bud essential oil: A new perspective as an eco-friendly biopesticide. in Agriculture
MDPI., 12(3), 338.
https://doi.org/10.3390/agriculture12030338

Milićević Z, Krnjajić S, Stević M, Ćirković J, Jelušić A, Pucarević M, Popović Milovanović T. Encapsulated clove bud essential oil: A new perspective as an eco-friendly biopesticide. in Agriculture. 2022;12(3):338.
doi:10.3390/agriculture12030338 .

Milićević, Zoran, Krnjajić, Slobodan, Stević, Milan, Ćirković, Jovana, Jelušić, Aleksandra, Pucarević, Mira, Popović Milovanović, Tatjana, "Encapsulated clove bud essential oil: A new perspective as an eco-friendly biopesticide" in Agriculture, 12, no. 3 (2022):338,
https://doi.org/10.3390/agriculture12030338 . .

TY  - JOUR
AU  - Jovanović, Jelena
AU  - Krnjajić, Slobodan
AU  - Ćirković, Jovana
AU  - Radojković, Aleksandar
AU  - Popović Milovanović, Tatjana
AU  - Branković, Goran
AU  - Branković, Zorica
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/743
AB  - The study reported the development of biodegradable, environment- and eco-friendly material based on natural polymers and lemongrass (Cymbopogon citratus L.) essential oil (LEO) for application as a green pesticide. The insecticidal effect of an emulsion composed of encapsulated LEO into a biopolymer matrix against the potato tuber moth (Phthorimaea operculella, Lepidoptera: Gelechiidae) (PTM) was examined and its prolonged effect compared with pure LEO. The lethal effect of the encapsulated LEO against the PTM was prolonged for up to seven days as compared to 48 h for the pure LEO. The slow release rate of the active compound (citral) from the encapsulated and the pure LEO was determined by UV-Vis spectrophotometry. After seven days the citral concentration in the encapsulated LEO was higher (60%) than in the pure LEO after 48 h (52.7%). The results of the slow release of the active compound correlated well with the insecticidal effect of the encapsulated and the pure LEO against the PTM.
PB  - Elsevier
T2  - Crop Protection
T1  - Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella
EP  - 109
SP  - 105
VL  - 132
DO  - 10.1016/j.cropro.2020.105109
ER  - 

@article{
author = "Jovanović, Jelena and Krnjajić, Slobodan and Ćirković, Jovana and Radojković, Aleksandar and Popović Milovanović, Tatjana and Branković, Goran and Branković, Zorica",
year = "2020",
abstract = "The study reported the development of biodegradable, environment- and eco-friendly material based on natural polymers and lemongrass (Cymbopogon citratus L.) essential oil (LEO) for application as a green pesticide. The insecticidal effect of an emulsion composed of encapsulated LEO into a biopolymer matrix against the potato tuber moth (Phthorimaea operculella, Lepidoptera: Gelechiidae) (PTM) was examined and its prolonged effect compared with pure LEO. The lethal effect of the encapsulated LEO against the PTM was prolonged for up to seven days as compared to 48 h for the pure LEO. The slow release rate of the active compound (citral) from the encapsulated and the pure LEO was determined by UV-Vis spectrophotometry. After seven days the citral concentration in the encapsulated LEO was higher (60%) than in the pure LEO after 48 h (52.7%). The results of the slow release of the active compound correlated well with the insecticidal effect of the encapsulated and the pure LEO against the PTM.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella",
pages = "109-105",
volume = "132",
doi = "10.1016/j.cropro.2020.105109"
}

Jovanović, J., Krnjajić, S., Ćirković, J., Radojković, A., Popović Milovanović, T., Branković, G.,& Branković, Z.. (2020). Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella. in Crop Protection
Elsevier., 132, 105-109.
https://doi.org/10.1016/j.cropro.2020.105109

Jovanović J, Krnjajić S, Ćirković J, Radojković A, Popović Milovanović T, Branković G, Branković Z. Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella. in Crop Protection. 2020;132:105-109.
doi:10.1016/j.cropro.2020.105109 .

Jovanović, Jelena, Krnjajić, Slobodan, Ćirković, Jovana, Radojković, Aleksandar, Popović Milovanović, Tatjana, Branković, Goran, Branković, Zorica, "Effect of encapsulated lemongrass (Cymbopogon citratus L.) essential oil against potato tuber moth Phthorimaea operculella" in Crop Protection, 132 (2020):105-109,
https://doi.org/10.1016/j.cropro.2020.105109 . .