TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Blagojević, Nevena
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1104
AB  - In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.
PB  - International Society for Horticultural Science
T2  - Acta Horticulturae
T1  - Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR
EP  - 84
SP  - 81
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.10
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Prokić, Anđelka and Blagojević, Nevena and Obradović, Aleksa and Gašić, Katarina",
year = "2014",
abstract = "In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.",
publisher = "International Society for Horticultural Science",
journal = "Acta Horticulturae",
title = "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR",
pages = "84-81",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.10"
}

Ivanović, M., Kuzmanović, N., Prokić, A., Blagojević, N., Obradović, A.,& Gašić, K.. (2014). Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae
International Society for Horticultural Science., 1056, 81-84.
https://doi.org/10.17660/ActaHortic.2014.1056.10

Ivanović M, Kuzmanović N, Prokić A, Blagojević N, Obradović A, Gašić K. Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in Acta Horticulturae. 2014;1056:81-84.
doi:10.17660/ActaHortic.2014.1056.10 .

Ivanović, Milan, Kuzmanović, Nemanja, Prokić, Anđelka, Blagojević, Nevena, Obradović, Aleksa, Gašić, Katarina, "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR" in Acta Horticulturae, 1056 (2014):81-84,
https://doi.org/10.17660/ActaHortic.2014.1056.10 . .

TY  - CONF
AU  - Ivanović, M.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Blagojević, Nevena
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/315
AB  - In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XIII International Workshop on Fire Blight
T1  - Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR
EP  - 84
SP  - 81
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.10
ER  - 

@conference{
author = "Ivanović, M. and Kuzmanović, Nemanja and Prokić, Anđelka and Blagojević, Nevena and Obradović, Aleksa and Gašić, Katarina",
year = "2014",
abstract = "In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR",
pages = "84-81",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.10"
}

Ivanović, M., Kuzmanović, N., Prokić, A., Blagojević, N., Obradović, A.,& Gašić, K.. (2014). Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 81-84.
https://doi.org/10.17660/ActaHortic.2014.1056.10

Ivanović M, Kuzmanović N, Prokić A, Blagojević N, Obradović A, Gašić K. Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in XIII International Workshop on Fire Blight. 2014;1056:81-84.
doi:10.17660/ActaHortic.2014.1056.10 .

Ivanović, M., Kuzmanović, Nemanja, Prokić, Anđelka, Blagojević, Nevena, Obradović, Aleksa, Gašić, Katarina, "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR" in XIII International Workshop on Fire Blight, 1056 (2014):81-84,
https://doi.org/10.17660/ActaHortic.2014.1056.10 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan M
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Blagojević, Nevena
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/299
AB  - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
EP  - 16
IS  - 1
SP  - 11
VL  - 137
DO  - 10.1007/s10658-013-0229-0
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ivanović, Milan M and Prokić, Anđelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa",
year = "2013",
abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia",
pages = "16-11",
number = "1",
volume = "137",
doi = "10.1007/s10658-013-0229-0"
}

Kuzmanović, N., Ivanović, M. M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 137(1), 11-16.
https://doi.org/10.1007/s10658-013-0229-0

Kuzmanović N, Ivanović MM, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16.
doi:10.1007/s10658-013-0229-0 .

Kuzmanović, Nemanja, Ivanović, Milan M, Prokić, Anđelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16,
https://doi.org/10.1007/s10658-013-0229-0 . .

TY  - JOUR
AU  - Obradović, Aleksa
AU  - Blagojević, Nevena
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1132
AB  - Proizvodnja, prerada i izvoz maline i kupine su u pojedinim regionima naše zemlje glavni izvor prihoda za većinu stanovništva. Međutim, profitabilnost proizvodnje često umanjuje pojava bolesti. U poređenju sa gljivičnim oboljenjima, bakterioze imaju manji značaj, ali pri vremenskim uslovima pogodnim za razvoj bolesti, mogu naneti ozbiljne štete. U radu su predstavljene najznačajnije bakterioze maline i kupine kao što su bakteriozna plamenjača, čiji je prouzrokovač Pseudomonas syringae, bakteriozni rak korena i izdanka, prouzrokovan bakterijom Agrobacterium tumefaciens, kao i bakteriozna plamenjača jabučastih voćaka prouzrokovana vrstom Erwinia amylovora, koja je opisana kao patogen maline u severnoj Americi. Osim bakterija, malinu i kupinu mogu parazitirati i fitoplazme. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja maline i kupine, kako bi se postavila pravilna i pravovremena dijagnoza i preduzele adekvatne mere zaštite.
PB  - Poljoprivredni fakultet, Novi Sad
T2  - Biljni lekar
T1  - Bakterioze i fitoplazmoze maline i kupine
EP  - 109
IS  - 2-3
SP  - 101
VL  - 40
ER  - 

@article{
author = "Obradović, Aleksa and Blagojević, Nevena",
year = "2012",
abstract = "Proizvodnja, prerada i izvoz maline i kupine su u pojedinim regionima naše zemlje glavni izvor prihoda za većinu stanovništva. Međutim, profitabilnost proizvodnje često umanjuje pojava bolesti. U poređenju sa gljivičnim oboljenjima, bakterioze imaju manji značaj, ali pri vremenskim uslovima pogodnim za razvoj bolesti, mogu naneti ozbiljne štete. U radu su predstavljene najznačajnije bakterioze maline i kupine kao što su bakteriozna plamenjača, čiji je prouzrokovač Pseudomonas syringae, bakteriozni rak korena i izdanka, prouzrokovan bakterijom Agrobacterium tumefaciens, kao i bakteriozna plamenjača jabučastih voćaka prouzrokovana vrstom Erwinia amylovora, koja je opisana kao patogen maline u severnoj Americi. Osim bakterija, malinu i kupinu mogu parazitirati i fitoplazme. Cilj rada je da doprinese poznavanju simptomatologije i epidemiologije bakterioznih i fitoplazmoznih oboljenja maline i kupine, kako bi se postavila pravilna i pravovremena dijagnoza i preduzele adekvatne mere zaštite.",
publisher = "Poljoprivredni fakultet, Novi Sad",
journal = "Biljni lekar",
title = "Bakterioze i fitoplazmoze maline i kupine",
pages = "109-101",
number = "2-3",
volume = "40"
}

Obradović, A.,& Blagojević, N.. (2012). Bakterioze i fitoplazmoze maline i kupine. in Biljni lekar
Poljoprivredni fakultet, Novi Sad., 40(2-3), 101-109.

Obradović A, Blagojević N. Bakterioze i fitoplazmoze maline i kupine. in Biljni lekar. 2012;40(2-3):101-109..

Obradović, Aleksa, Blagojević, Nevena, "Bakterioze i fitoplazmoze maline i kupine" in Biljni lekar, 40, no. 2-3 (2012):101-109.