TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan M
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Blagojević, Nevena
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/299
AB  - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
EP  - 16
IS  - 1
SP  - 11
VL  - 137
DO  - 10.1007/s10658-013-0229-0
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ivanović, Milan M and Prokić, Anđelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa",
year = "2013",
abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia",
pages = "16-11",
number = "1",
volume = "137",
doi = "10.1007/s10658-013-0229-0"
}

Kuzmanović, N., Ivanović, M. M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 137(1), 11-16.
https://doi.org/10.1007/s10658-013-0229-0

Kuzmanović N, Ivanović MM, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16.
doi:10.1007/s10658-013-0229-0 .

Kuzmanović, Nemanja, Ivanović, Milan M, Prokić, Anđelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16,
https://doi.org/10.1007/s10658-013-0229-0 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Ivanović, Milan M
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/201
AB  - In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.
PB  - University of Belgrade, University of Novi Sad
T2  - Archives of Biological Sciences
T1  - Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia
EP  - 1494
IS  - 4
SP  - 1487
VL  - 64
DO  - 10.2298/ABS1204487K
ER  - 

@article{
author = "Kuzmanović, Nemanja and Gašić, Katarina and Ivanović, Milan M and Prokić, Anđelka and Obradović, Aleksa",
year = "2012",
abstract = "In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia",
pages = "1494-1487",
number = "4",
volume = "64",
doi = "10.2298/ABS1204487K"
}

Kuzmanović, N., Gašić, K., Ivanović, M. M., Prokić, A.,& Obradović, A.. (2012). Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 64(4), 1487-1494.
https://doi.org/10.2298/ABS1204487K

Kuzmanović N, Gašić K, Ivanović MM, Prokić A, Obradović A. Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia. in Archives of Biological Sciences. 2012;64(4):1487-1494.
doi:10.2298/ABS1204487K .

Kuzmanović, Nemanja, Gašić, Katarina, Ivanović, Milan M, Prokić, Anđelka, Obradović, Aleksa, "Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1487-1494,
https://doi.org/10.2298/ABS1204487K . .

TY  - JOUR
AU  - Ivanović, Milan M
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V
AU  - Dickstein, Ellen R
AU  - Jones, Jeffrey B
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/219
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Erratum to: Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)
EP  - 443
IS  - 2
SP  - 443
VL  - 134
DO  - 10.1007/s10658-012-0037-y
ER  - 

@article{
author = "Ivanović, Milan M and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V and Dickstein, Ellen R and Jones, Jeffrey B",
year = "2012",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Erratum to: Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)",
pages = "443-443",
number = "2",
volume = "134",
doi = "10.1007/s10658-012-0037-y"
}

Ivanović, M. M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Erratum to: Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology
Springer, Dordrecht., 134(2), 443-443.
https://doi.org/10.1007/s10658-012-0037-y

Ivanović MM, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Erratum to: Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012). in European Journal of Plant Pathology. 2012;134(2):443-443.
doi:10.1007/s10658-012-0037-y .

Ivanović, Milan M, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V, Dickstein, Ellen R, Jones, Jeffrey B, "Erratum to: Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns (vol 133, pg 545, 2012)" in European Journal of Plant Pathology, 134, no. 2 (2012):443-443,
https://doi.org/10.1007/s10658-012-0037-y . .

TY  - JOUR
AU  - Ivanović, Milan M
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Minsavage, Gerald V
AU  - Dickstein, Ellen R
AU  - Jones, Jeffrey B
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/213
AB  - Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns
EP  - 727
IS  - 3
SP  - 715
VL  - 133
DO  - 10.1007/s10658-012-9950-3
ER  - 

@article{
author = "Ivanović, Milan M and Obradović, Aleksa and Gašić, Katarina and Minsavage, Gerald V and Dickstein, Ellen R and Jones, Jeffrey B",
year = "2012",
abstract = "Forty Erwinia amylovora strains originating from different host plants and locations in Serbia and one strain from Montenegro were characterized by conventional, automated and molecular techniques. All strains were Gram-negative, nonfluorescent, facultative anaerobes, oxidase negative, levan positive, produced necrotic lesions followed by bacterial exudate on artificially inoculated immature pear fruits and caused HR on tobacco. Based on carbon source utilization, all strains tested with the Biolog system were identified as E. amylovora. Based on fatty acid profiles all tested strains clustered into three groups in which strains from north Serbia differed from strains isolated in central and south parts of the country. Restriction analysis of genomic DNA using XbaI and PFGE resulted in six different patterns differentiating the strains into six groups. Most of the investigated strains clustered in one group having the pattern type similar to Pt2 group described earlier as dominant in East Europe and the Mediterranean region. Two strains showed PFGE pattern similar to the previously described Pt3 pattern and one strain had pattern similar to Pt6. Based on size and number of the bands, new restriction patterns, assigned as Pt7, Pt8 and Pt9 were observed. PFGE results showed that E. amylovora population in Serbia is not homogenous and was possibly introduced from different directions. This is the first characterization of E. amylovora collection of strains from Serbia using fatty acid analysis and PFGE.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns",
pages = "727-715",
number = "3",
volume = "133",
doi = "10.1007/s10658-012-9950-3"
}

Ivanović, M. M., Obradović, A., Gašić, K., Minsavage, G. V., Dickstein, E. R.,& Jones, J. B.. (2012). Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology
Springer, Dordrecht., 133(3), 715-727.
https://doi.org/10.1007/s10658-012-9950-3

Ivanović MM, Obradović A, Gašić K, Minsavage GV, Dickstein ER, Jones JB. Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns. in European Journal of Plant Pathology. 2012;133(3):715-727.
doi:10.1007/s10658-012-9950-3 .

Ivanović, Milan M, Obradović, Aleksa, Gašić, Katarina, Minsavage, Gerald V, Dickstein, Ellen R, Jones, Jeffrey B, "Exploring diversity of Erwinia amylovora population in Serbia by conventional and automated techniques and detection of new PFGE patterns" in European Journal of Plant Pathology, 133, no. 3 (2012):715-727,
https://doi.org/10.1007/s10658-012-9950-3 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Ivanović, Milan M
AU  - Ignjatov, Maja
AU  - Calić, A
AU  - Obradović, Aleksa
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/160
AB  - Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Isolation and characterization of Xanthomonas euvesicatoria bacteriophages
EP  - 423
IS  - 2
SP  - 415
VL  - 93
ER  - 

@article{
author = "Gašić, Katarina and Ivanović, Milan M and Ignjatov, Maja and Calić, A and Obradović, Aleksa",
year = "2011",
abstract = "Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Isolation and characterization of Xanthomonas euvesicatoria bacteriophages",
pages = "423-415",
number = "2",
volume = "93"
}

Gašić, K., Ivanović, M. M., Ignjatov, M., Calić, A.,& Obradović, A.. (2011). Isolation and characterization of Xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology
Edizioni Ets, Pisa., 93(2), 415-423.

Gašić K, Ivanović MM, Ignjatov M, Calić A, Obradović A. Isolation and characterization of Xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology. 2011;93(2):415-423..

Gašić, Katarina, Ivanović, Milan M, Ignjatov, Maja, Calić, A, Obradović, Aleksa, "Isolation and characterization of Xanthomonas euvesicatoria bacteriophages" in Journal of Plant Pathology, 93, no. 2 (2011):415-423.