TY  - CONF
AU  - Cvrković, Tatjana
AU  - Jović, Jelena
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1214
AB  - “Flavescence dorée” (FD) is a European quarantine disease affecting grapevines,
associated with the “flavescence dorée” phytoplasma and transmitted by
the North American leafhopper, Scaphoideus titanus. This pathogen has a
significant impact on the major viticultural areas across Europe, as well as all
wine-producing regions in Serbia. The occurrence of S. titanus was initially
recorded in Serbian vineyards in 2002 (Magud and Toševski, 2004). However,
the high population density observed at that time suggests that the vector’s
presence likely dates back to the 1990s (Ivo Toševski, unpublished data).
Despite the extensive measures to contain the pathogen, the encompassing
disease and vector surveillance, the mandatory insecticidal treatments against
S. titanus, and the utilization of certified phytoplasma-free planting material,
the disease continues to spread. Presently, FD affects vineyards in all districts of
Serbia (Krstić et al., 2022). A recent two-year survey (2022-2023) on S. titanus
distribution in both wild and cultivated areas within vineyard ecosystems
revealed widespread prevalence of the vector across all grapevine-growing
regions in Serbia. High population densities were observed in districts where
Map-FD3 genotypes are common. These include grape-producing regions all
over Serbia where the autochthonous M51 genotype is dominant, hosted by
grapevine, along with reservoir plants Clematis vitalba and Ailanthus altissima.
Additionally, substantial S. titanus populations were registered in northern
Serbia, where multiple outbreaks of the M12 genotype occurred, and central
Serbia, where five new genotypes (M150-M154) were detected in grapevines,
indicating a high endemic potential for new outbreaks in these regions. Finally,
significant populations of the insect vector were discovered in riparian habitats
near rivers in Podunavlje and Mačva, where two “flavescence dorée” endemic genotypes of the Map-FD2 (M89 and M148) were found in grapevines (Krstić
et al., 2022). The current phytosanitary situation in Serbian vineyards provides
evidence that, despite measures implemented over the past two decades, there
has been no substantial reduction in the population density and spread of S.
titanus. Due to the high complexity of the “flavescence dorée” epidemiological
cycle caused by natural reservoir plants and native or introduced insect vectors,
linking natural habitats with adjacent vineyard ecosystems, managing ongoing
epidemics and preventing new outbreaks in the Balkans remains a challenge.
C3  - European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy
T1  - Status of Scaphoideus titanus in Serbia vineyards two decades later
EP  - 42
SP  - 41
ER  - 

@conference{
author = "Cvrković, Tatjana and Jović, Jelena and Jakovljević, Miljana and Krstić, Oliver and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo",
year = "2024",
abstract = "“Flavescence dorée” (FD) is a European quarantine disease affecting grapevines,
associated with the “flavescence dorée” phytoplasma and transmitted by
the North American leafhopper, Scaphoideus titanus. This pathogen has a
significant impact on the major viticultural areas across Europe, as well as all
wine-producing regions in Serbia. The occurrence of S. titanus was initially
recorded in Serbian vineyards in 2002 (Magud and Toševski, 2004). However,
the high population density observed at that time suggests that the vector’s
presence likely dates back to the 1990s (Ivo Toševski, unpublished data).
Despite the extensive measures to contain the pathogen, the encompassing
disease and vector surveillance, the mandatory insecticidal treatments against
S. titanus, and the utilization of certified phytoplasma-free planting material,
the disease continues to spread. Presently, FD affects vineyards in all districts of
Serbia (Krstić et al., 2022). A recent two-year survey (2022-2023) on S. titanus
distribution in both wild and cultivated areas within vineyard ecosystems
revealed widespread prevalence of the vector across all grapevine-growing
regions in Serbia. High population densities were observed in districts where
Map-FD3 genotypes are common. These include grape-producing regions all
over Serbia where the autochthonous M51 genotype is dominant, hosted by
grapevine, along with reservoir plants Clematis vitalba and Ailanthus altissima.
Additionally, substantial S. titanus populations were registered in northern
Serbia, where multiple outbreaks of the M12 genotype occurred, and central
Serbia, where five new genotypes (M150-M154) were detected in grapevines,
indicating a high endemic potential for new outbreaks in these regions. Finally,
significant populations of the insect vector were discovered in riparian habitats
near rivers in Podunavlje and Mačva, where two “flavescence dorée” endemic genotypes of the Map-FD2 (M89 and M148) were found in grapevines (Krstić
et al., 2022). The current phytosanitary situation in Serbian vineyards provides
evidence that, despite measures implemented over the past two decades, there
has been no substantial reduction in the population density and spread of S.
titanus. Due to the high complexity of the “flavescence dorée” epidemiological
cycle caused by natural reservoir plants and native or introduced insect vectors,
linking natural habitats with adjacent vineyard ecosystems, managing ongoing
epidemics and preventing new outbreaks in the Balkans remains a challenge.",
journal = "European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy",
title = "Status of Scaphoideus titanus in Serbia vineyards two decades later",
pages = "42-41"
}

Cvrković, T., Jović, J., Jakovljević, M., Krstić, O., Marinković, S., Mitrović, M.,& Tosevski, I.. (2024). Status of Scaphoideus titanus in Serbia vineyards two decades later. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy, 41-42.

Cvrković T, Jović J, Jakovljević M, Krstić O, Marinković S, Mitrović M, Tosevski I. Status of Scaphoideus titanus in Serbia vineyards two decades later. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy. 2024;:41-42..

Cvrković, Tatjana, Jović, Jelena, Jakovljević, Miljana, Krstić, Oliver, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, "Status of Scaphoideus titanus in Serbia vineyards two decades later" in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy (2024):41-42.

TY  - CONF
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1215
AB  - “Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.
C3  - European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy
T1  - Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia
EP  - 54
SP  - 53
ER  - 

@conference{
author = "Jović, Jelena and Cvrković, Tatjana and Krstić, Oliver and Mitrović, Milana and Jakovljević, Miljana and Marinković, Slavica and Toševski, Ivo",
year = "2024",
abstract = "“Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.",
journal = "European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy",
title = "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia",
pages = "54-53"
}

Jović, J., Cvrković, T., Krstić, O., Mitrović, M., Jakovljević, M., Marinković, S.,& Toševski, I.. (2024). Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy, 53-54.

Jović J, Cvrković T, Krstić O, Mitrović M, Jakovljević M, Marinković S, Toševski I. Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy. 2024;:53-54..

Jović, Jelena, Cvrković, Tatjana, Krstić, Oliver, Mitrović, Milana, Jakovljević, Miljana, Marinković, Slavica, Toševski, Ivo, "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia" in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy (2024):53-54.

TY  - CONF
AU  - Delač, Magdalena
AU  - Polak, Bruno
AU  - Drčelić, Marina
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Šeruga Musić, Martina
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1216
AB  - Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.
C3  - Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023
T1  - Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich
EP  - 185
SP  - 184
ER  - 

@conference{
author = "Delač, Magdalena and Polak, Bruno and Drčelić, Marina and Jović, Jelena and Krstić, Oliver and Toševski, Ivo and Cvrković, Tatjana and Jakovljević, Miljana and Šeruga Musić, Martina",
year = "2023",
abstract = "Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.",
journal = "Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023",
title = "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich",
pages = "185-184"
}

Delač, M., Polak, B., Drčelić, M., Jović, J., Krstić, O., Toševski, I., Cvrković, T., Jakovljević, M.,& Šeruga Musić, M.. (2023). Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023, 184-185.

Delač M, Polak B, Drčelić M, Jović J, Krstić O, Toševski I, Cvrković T, Jakovljević M, Šeruga Musić M. Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023. 2023;:184-185..

Delač, Magdalena, Polak, Bruno, Drčelić, Marina, Jović, Jelena, Krstić, Oliver, Toševski, Ivo, Cvrković, Tatjana, Jakovljević, Miljana, Šeruga Musić, Martina, "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich" in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023 (2023):184-185.

TY  - JOUR
AU  - Gosik, Rafał
AU  - Sprick, Peter
AU  - Wrzesien, Malgorzata
AU  - Dzyr, Agata
AU  - Krstić, Oliver
AU  - Tosevski, Ivo
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/711
AB  - The mature larva and pupa of Otiorhynchus smreczynskii are described and illustrated with complete chaetotaxy for the first time. Five larval instars and the factors for larval growth are determined, and the larval development of this species is comprehensively described. In order to confirm species affiliation, selected larvae were subjected to genetic analysis (mtCOI). Host plants and unique feeding signs of some Entiminae species are presented, and all available data on development are documented and interpreted. Additionally, the morphometry of 78 specimens (48 of O. smreczynskii and 30 of O. rotundus) was examined in order to verify the usefulness of morphological features in distinguishing both species. The female genitalia of both species are illustrated, described and compared with each other for the first time. Finally, the updated distribution of O. smreczynskii is given, and a possible origin of O. smreczynskii and O. rotundus is proposed.
PB  - MDPI Basel
T2  - INSECTS
T1  - Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution
IS  - 4
VL  - 14
DO  - 10.3390/insects14040360
ER  - 

@article{
author = "Gosik, Rafał and Sprick, Peter and Wrzesien, Malgorzata and Dzyr, Agata and Krstić, Oliver and Tosevski, Ivo",
year = "2023",
abstract = "The mature larva and pupa of Otiorhynchus smreczynskii are described and illustrated with complete chaetotaxy for the first time. Five larval instars and the factors for larval growth are determined, and the larval development of this species is comprehensively described. In order to confirm species affiliation, selected larvae were subjected to genetic analysis (mtCOI). Host plants and unique feeding signs of some Entiminae species are presented, and all available data on development are documented and interpreted. Additionally, the morphometry of 78 specimens (48 of O. smreczynskii and 30 of O. rotundus) was examined in order to verify the usefulness of morphological features in distinguishing both species. The female genitalia of both species are illustrated, described and compared with each other for the first time. Finally, the updated distribution of O. smreczynskii is given, and a possible origin of O. smreczynskii and O. rotundus is proposed.",
publisher = "MDPI Basel",
journal = "INSECTS",
title = "Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution",
number = "4",
volume = "14",
doi = "10.3390/insects14040360"
}

Gosik, R., Sprick, P., Wrzesien, M., Dzyr, A., Krstić, O.,& Tosevski, I.. (2023). Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution. in INSECTS
MDPI Basel., 14(4).
https://doi.org/10.3390/insects14040360

Gosik R, Sprick P, Wrzesien M, Dzyr A, Krstić O, Tosevski I. Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution. in INSECTS. 2023;14(4).
doi:10.3390/insects14040360 .

Gosik, Rafał, Sprick, Peter, Wrzesien, Malgorzata, Dzyr, Agata, Krstić, Oliver, Tosevski, Ivo, "Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution" in INSECTS, 14, no. 4 (2023),
https://doi.org/10.3390/insects14040360 . .

TY  - JOUR
AU  - Tosevski, Ivo
AU  - Sing, Sharlene
AU  - Caldara, Roberto
AU  - Weaver, David
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Hinz, Hariet
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/709
AB  - A seed-feeding weevil introduced to North America (NA) as a biological control agent of the invasive toadflax Linaria dalmatica (L.) Mill., identified then as Gymnetron antirrhini “Dalmatian host race” and subsequently confirmed as established, was revealed through our study to be a separate species, i.e., Rhinusa dieckmanni (Behne) (Coleoptera: Curculionidae). This weevil species was presumed to be endemic in its native range, with a distribution restricted to Mount Rila in southwestern Bulgaria. We conducted a comprehensive study of seed-feeding weevils associated with L. dalmatica, L. dalmatica ssp. macedonica (Griseb.) D.A. Sutton, L. genistifolia (L.) Mill., and L. grandiflora Desf. across a broad geographic area of their native range. Those results revealed that all four host plants were used by R. dieckmanni and thus the native geographic range of the species is wider than expected, encompassing the Balkans and the Anatolian Plateau. Our observations suggest that phenotypes of this weevil are highly variable and dependent on the seed capsule size of the Linaria host population. The haplotype network based on mitochondrial COII, 16S genes, and nuclear EF 1-α gene genealogy confirmed the conspecific nature of geographically distant weevil populations, that is, R. dieckmanni phenotypes utilizing L. genistifolia, L. dalmatica, and L. grandiflora for larval development. Specimens collected from L. dalmatica in the northwestern USA shared the same haplotypes as samples from L. dalmatica ssp. macedonica in southwestern North Macedonia, supporting the known introduction history of the North American population. Females from these populations have relatively short rostrums, which may limit their reproductive success on North American invasive L. dalmatica with larger seed capsules.
PB  - Elsevier
T2  - Biological Control
T1  - Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America
VL  - 183
DO  - 10.1016/j.biocontrol.2023.105270
ER  - 

@article{
author = "Tosevski, Ivo and Sing, Sharlene and Caldara, Roberto and Weaver, David and Jovic, Jelena and Krstić, Oliver and Hinz, Hariet",
year = "2023",
abstract = "A seed-feeding weevil introduced to North America (NA) as a biological control agent of the invasive toadflax Linaria dalmatica (L.) Mill., identified then as Gymnetron antirrhini “Dalmatian host race” and subsequently confirmed as established, was revealed through our study to be a separate species, i.e., Rhinusa dieckmanni (Behne) (Coleoptera: Curculionidae). This weevil species was presumed to be endemic in its native range, with a distribution restricted to Mount Rila in southwestern Bulgaria. We conducted a comprehensive study of seed-feeding weevils associated with L. dalmatica, L. dalmatica ssp. macedonica (Griseb.) D.A. Sutton, L. genistifolia (L.) Mill., and L. grandiflora Desf. across a broad geographic area of their native range. Those results revealed that all four host plants were used by R. dieckmanni and thus the native geographic range of the species is wider than expected, encompassing the Balkans and the Anatolian Plateau. Our observations suggest that phenotypes of this weevil are highly variable and dependent on the seed capsule size of the Linaria host population. The haplotype network based on mitochondrial COII, 16S genes, and nuclear EF 1-α gene genealogy confirmed the conspecific nature of geographically distant weevil populations, that is, R. dieckmanni phenotypes utilizing L. genistifolia, L. dalmatica, and L. grandiflora for larval development. Specimens collected from L. dalmatica in the northwestern USA shared the same haplotypes as samples from L. dalmatica ssp. macedonica in southwestern North Macedonia, supporting the known introduction history of the North American population. Females from these populations have relatively short rostrums, which may limit their reproductive success on North American invasive L. dalmatica with larger seed capsules.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America",
volume = "183",
doi = "10.1016/j.biocontrol.2023.105270"
}

Tosevski, I., Sing, S., Caldara, R., Weaver, D., Jovic, J., Krstić, O.,& Hinz, H.. (2023). Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America. in Biological Control
Elsevier., 183.
https://doi.org/10.1016/j.biocontrol.2023.105270

Tosevski I, Sing S, Caldara R, Weaver D, Jovic J, Krstić O, Hinz H. Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America. in Biological Control. 2023;183.
doi:10.1016/j.biocontrol.2023.105270 .

Tosevski, Ivo, Sing, Sharlene, Caldara, Roberto, Weaver, David, Jovic, Jelena, Krstić, Oliver, Hinz, Hariet, "Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America" in Biological Control, 183 (2023),
https://doi.org/10.1016/j.biocontrol.2023.105270 . .

TY  - JOUR
AU  - Radonjić, Sanja
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Hrnčić, Snježana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/691
AB  - Abstract Flavescence dorée (FD) is a severe and epidemic grapevine yellows disease, affecting most of the major European viticultural regions and classified as a quarantine pest. It is transmitted primarily by the ampelophagous Neartic deltocephalinae leafhopper, Scaphoideus titanus. The causative agent of the disease is FD phytoplasma (FDp), Vectotypes II and III, member of the 16S rRNA taxonomic subgroups V-C and V-D. Recent evidence indicates that FDp is native to Europe. Since 2018, vineyards in Montenegro have been subject to a preventive surveillance for the presence of FDp. In July of 2021, 56 samples of symptomatic grapevines were collected from vineyards in the major wine-growing regions. Using conventional nested PCR on the 16SrV group-specific FD9 marker (secY gene) and PCR-RFLP analysis of the 16S gene, the 16SrV-C phytoplasma subgroup was identified in a single grapevine from the Godinje location of the Crmnica wine subregion. The isolate was identified as Vectotype III, Map-FD3/VmpA-III genotype M51/III-c11 after characterizing the epidemiologicaly informative map and vmpA genes. In October of the same year, additional grapevines and potential reservoir or natural host plants of FDp and related phytoplasmas were sampled in the Godinje location. No new FDp-infected grapevines were found, indicating that this was likely an incidental transmission by natural, indigenous vectors and not an outbreak. However, one clematis and six alders were found carrying 16SrV group phytoplasmas and were subjected to map/vmpA typing, along with isolates of previously collected clematis plants in the Nudo wine region, and alder trees in non-viticultural areas and the potential wine region of Montenegrin North. The clematis plant in Godinje shared the same FDp Vectotype III as the infected vine, suggesting that clematis was the likely source of infection. On the other hand, alders in Godinje harbored VmpA-II cluster genotypes of either Map-FD2 M148 or AldY (alder yellows) M52, or multiple map gene sequences. Finally, all three FDp clusters of epidemic genotypes were found in clematis and alder reservoir plants in various regions of Montenegro. According to Map/VmpA Vectotype classification, clematis was identified as a potential source of FD3/III-c, and alders of FD1/III-f and FD2/II-d cluster genotypes. These findings call for stringent management and monitoring of S. titanus in Montenegrin vineyards to prevent FDp epidemic outbreaks.
PB  - Springer Verlag
T2  - JOURNAL OF PLANT PATHOLOGY
T1  - The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs
EP  - 427
IS  - 2
SP  - 419
VL  - 105
DO  - 10.1007/s42161-023-01318-z
ER  - 

@article{
author = "Radonjić, Sanja and Krstić, Oliver and Cvrković, Tatjana and Hrnčić, Snježana and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo and Jovic, Jelena",
year = "2023",
abstract = "Abstract Flavescence dorée (FD) is a severe and epidemic grapevine yellows disease, affecting most of the major European viticultural regions and classified as a quarantine pest. It is transmitted primarily by the ampelophagous Neartic deltocephalinae leafhopper, Scaphoideus titanus. The causative agent of the disease is FD phytoplasma (FDp), Vectotypes II and III, member of the 16S rRNA taxonomic subgroups V-C and V-D. Recent evidence indicates that FDp is native to Europe. Since 2018, vineyards in Montenegro have been subject to a preventive surveillance for the presence of FDp. In July of 2021, 56 samples of symptomatic grapevines were collected from vineyards in the major wine-growing regions. Using conventional nested PCR on the 16SrV group-specific FD9 marker (secY gene) and PCR-RFLP analysis of the 16S gene, the 16SrV-C phytoplasma subgroup was identified in a single grapevine from the Godinje location of the Crmnica wine subregion. The isolate was identified as Vectotype III, Map-FD3/VmpA-III genotype M51/III-c11 after characterizing the epidemiologicaly informative map and vmpA genes. In October of the same year, additional grapevines and potential reservoir or natural host plants of FDp and related phytoplasmas were sampled in the Godinje location. No new FDp-infected grapevines were found, indicating that this was likely an incidental transmission by natural, indigenous vectors and not an outbreak. However, one clematis and six alders were found carrying 16SrV group phytoplasmas and were subjected to map/vmpA typing, along with isolates of previously collected clematis plants in the Nudo wine region, and alder trees in non-viticultural areas and the potential wine region of Montenegrin North. The clematis plant in Godinje shared the same FDp Vectotype III as the infected vine, suggesting that clematis was the likely source of infection. On the other hand, alders in Godinje harbored VmpA-II cluster genotypes of either Map-FD2 M148 or AldY (alder yellows) M52, or multiple map gene sequences. Finally, all three FDp clusters of epidemic genotypes were found in clematis and alder reservoir plants in various regions of Montenegro. According to Map/VmpA Vectotype classification, clematis was identified as a potential source of FD3/III-c, and alders of FD1/III-f and FD2/II-d cluster genotypes. These findings call for stringent management and monitoring of S. titanus in Montenegrin vineyards to prevent FDp epidemic outbreaks.",
publisher = "Springer Verlag",
journal = "JOURNAL OF PLANT PATHOLOGY",
title = "The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs",
pages = "427-419",
number = "2",
volume = "105",
doi = "10.1007/s42161-023-01318-z"
}

Radonjić, S., Krstić, O., Cvrković, T., Hrnčić, S., Marinković, S., Mitrović, M., Tosevski, I.,& Jovic, J.. (2023). The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs. in JOURNAL OF PLANT PATHOLOGY
Springer Verlag., 105(2), 419-427.
https://doi.org/10.1007/s42161-023-01318-z

Radonjić S, Krstić O, Cvrković T, Hrnčić S, Marinković S, Mitrović M, Tosevski I, Jovic J. The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs. in JOURNAL OF PLANT PATHOLOGY. 2023;105(2):419-427.
doi:10.1007/s42161-023-01318-z .

Radonjić, Sanja, Krstić, Oliver, Cvrković, Tatjana, Hrnčić, Snježana, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, Jovic, Jelena, "The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs" in JOURNAL OF PLANT PATHOLOGY, 105, no. 2 (2023):419-427,
https://doi.org/10.1007/s42161-023-01318-z . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/719
AB  - Flavescence dorée (FD) is a European quarantine disease of grapevine caused by FD phytoplasma (FDp) transmitted by the leafhopper of North American origin Scaphoideus titanus. The disease affects the most important viticultural regions of Europe and all wine-growing regions of Serbia. Unlike the insect vector, the pathogen is native to Europe and associated with several wild host plants among which alder trees as the main source of two out of three map genetic clusters of pathogen variants (Map-FD1 and FD2). Heretofore, the FDp epidemic in Serbian vineyards was thought to be monotypic, i.e., caused by the single genotype of the Map-FD3 cluster, M51, and correlated with clematis as the natural source plant. This study aimed to provide data on genetic diversity, through map and vmpA gene typing, and insights into ecological properties of epidemiological cycles driving the epidemic outbreaks of FD in Serbia today. Map genotyping of 270 grapevine isolates collected from 2017 to 2019 confirmed M51 as autochthonous genotype widespread in all wine producing regions of Serbia and the dominant FDp epidemic genotype in most of the districts (75%, 202/270 isolates), except in north Serbia where multiple outbreaks of M12 Map-FD3 were recorded (54 isolates). Tree of heaven is reported as a new FDp plant reservoir for the Serbian vineyards, hosting the M51 genotype, along with clematis. An outbreak of a new endemic Map-FD3 genotype M144 was documented in grapevine samples from east Serbia (5 isolates), correlating with previous findings of the same genotype in clematis. In addition, single grapevine infections with five new Map-FD3 genotypes (M150-M154) were recorded in central Serbia, thus indicating high endemic potential for new outbreaks. The vmpA typing placed all Map-FD3 isolates into the VmpA-III cluster, i.e., Vectotype III. Finally, we found direct evidence that at least two FDp endemic genotypes, M89 and M148, of the Map-FD2/VmpA-II have escaped from alders and propagated in the grapevine-S. titanus pathosystem in Serbia (Vectotype II). Our findings confirm the high complexity of the FDp ecological cycle and provide evidence of a unique, autochthonous Balkan epidemiology sourced endemically.
PB  - MDPI Basel
T2  - Agronomy
T1  - Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes
IS  - 2
VL  - 12
DO  - 10.3390/agronomy12020448
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo and Jovic, Jelena",
year = "2022",
abstract = "Flavescence dorée (FD) is a European quarantine disease of grapevine caused by FD phytoplasma (FDp) transmitted by the leafhopper of North American origin Scaphoideus titanus. The disease affects the most important viticultural regions of Europe and all wine-growing regions of Serbia. Unlike the insect vector, the pathogen is native to Europe and associated with several wild host plants among which alder trees as the main source of two out of three map genetic clusters of pathogen variants (Map-FD1 and FD2). Heretofore, the FDp epidemic in Serbian vineyards was thought to be monotypic, i.e., caused by the single genotype of the Map-FD3 cluster, M51, and correlated with clematis as the natural source plant. This study aimed to provide data on genetic diversity, through map and vmpA gene typing, and insights into ecological properties of epidemiological cycles driving the epidemic outbreaks of FD in Serbia today. Map genotyping of 270 grapevine isolates collected from 2017 to 2019 confirmed M51 as autochthonous genotype widespread in all wine producing regions of Serbia and the dominant FDp epidemic genotype in most of the districts (75%, 202/270 isolates), except in north Serbia where multiple outbreaks of M12 Map-FD3 were recorded (54 isolates). Tree of heaven is reported as a new FDp plant reservoir for the Serbian vineyards, hosting the M51 genotype, along with clematis. An outbreak of a new endemic Map-FD3 genotype M144 was documented in grapevine samples from east Serbia (5 isolates), correlating with previous findings of the same genotype in clematis. In addition, single grapevine infections with five new Map-FD3 genotypes (M150-M154) were recorded in central Serbia, thus indicating high endemic potential for new outbreaks. The vmpA typing placed all Map-FD3 isolates into the VmpA-III cluster, i.e., Vectotype III. Finally, we found direct evidence that at least two FDp endemic genotypes, M89 and M148, of the Map-FD2/VmpA-II have escaped from alders and propagated in the grapevine-S. titanus pathosystem in Serbia (Vectotype II). Our findings confirm the high complexity of the FDp ecological cycle and provide evidence of a unique, autochthonous Balkan epidemiology sourced endemically.",
publisher = "MDPI Basel",
journal = "Agronomy",
title = "Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes",
number = "2",
volume = "12",
doi = "10.3390/agronomy12020448"
}

Krstić, O., Cvrković, T., Marinković, S., Jakovljević, M., Mitrović, M., Tosevski, I.,& Jovic, J.. (2022). Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes. in Agronomy
MDPI Basel., 12(2).
https://doi.org/10.3390/agronomy12020448

Krstić O, Cvrković T, Marinković S, Jakovljević M, Mitrović M, Tosevski I, Jovic J. Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes. in Agronomy. 2022;12(2).
doi:10.3390/agronomy12020448 .

Krstić, Oliver, Cvrković, Tatjana, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, Jovic, Jelena, "Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes" in Agronomy, 12, no. 2 (2022),
https://doi.org/10.3390/agronomy12020448 . .

TY  - JOUR
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/718
AB  - Bois noir, an economically important disease of grapevine yellows that causes significant economic losses in wine production, is associated with ‘Candidatus Phytoplasma solani’ and transmitted to grapevines by cixiids Hyalesthes obsoletus and Reptalus panzeri. Polyphagous planthopper Dictyophara europaea, commonly found in natural habitats, harbors phytoplasmas from distinct groups and is an alternative vector in the open epidemiological cycles of the Flavescence dorée phytoplasma in grapevine in European vineyards. This study addresses the role of D. europaea in the transmission cycle(s) of ‘Ca. P. solani’ among wild habitats, natural reservoir plants, and the vineyard agroecosystem using MLSA and transmission trials with naturally infected adults to grapevine and Catharanthus roseus. The infection rates of D. europaea ranged from 7% to 13% in diverse locations, while reservoir herbaceous plants were infected in the amount of 29%. A total of 13 CaPsol MLSA genotypes were detected in D. europaea (7) and plants (8). Nine of them corresponded to previously identified genotypes. Two new genotypes were found in D. europaea (tuf-b1/S1/V14/Rqg50-sv1 and tuf-b1/S18/V14/Rqg50-sv1) and one in Convolvulus arvensis (tuf-b1/S1/V2-TA/Rqg31-sv1), whereas one was shared by two hosts, Crepis foetida and Daucus carota (tuf-b1/S1/V2-TA/STOL-sv1). Naturally infected D. europaea successfully transmitted the tuf-b1/S1/V2-TA/STOL type to five grapevines and six periwinkles, tuf-b1/S1/V2-TA/Rqg31 to one grapevine, and tuf-b1/S1/V2-TA/Rqg50 to one periwinkle, indicating that D. europaea is an intermediate vector in CaPsol epidemiological cycles.
PB  - MDPI Basel
T2  - Horticulturae
T1  - Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’
IS  - 7
SP  - 654
VL  - 8
DO  - 10.3390/horticulturae8070654
ER  - 

@article{
author = "Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
abstract = "Bois noir, an economically important disease of grapevine yellows that causes significant economic losses in wine production, is associated with ‘Candidatus Phytoplasma solani’ and transmitted to grapevines by cixiids Hyalesthes obsoletus and Reptalus panzeri. Polyphagous planthopper Dictyophara europaea, commonly found in natural habitats, harbors phytoplasmas from distinct groups and is an alternative vector in the open epidemiological cycles of the Flavescence dorée phytoplasma in grapevine in European vineyards. This study addresses the role of D. europaea in the transmission cycle(s) of ‘Ca. P. solani’ among wild habitats, natural reservoir plants, and the vineyard agroecosystem using MLSA and transmission trials with naturally infected adults to grapevine and Catharanthus roseus. The infection rates of D. europaea ranged from 7% to 13% in diverse locations, while reservoir herbaceous plants were infected in the amount of 29%. A total of 13 CaPsol MLSA genotypes were detected in D. europaea (7) and plants (8). Nine of them corresponded to previously identified genotypes. Two new genotypes were found in D. europaea (tuf-b1/S1/V14/Rqg50-sv1 and tuf-b1/S18/V14/Rqg50-sv1) and one in Convolvulus arvensis (tuf-b1/S1/V2-TA/Rqg31-sv1), whereas one was shared by two hosts, Crepis foetida and Daucus carota (tuf-b1/S1/V2-TA/STOL-sv1). Naturally infected D. europaea successfully transmitted the tuf-b1/S1/V2-TA/STOL type to five grapevines and six periwinkles, tuf-b1/S1/V2-TA/Rqg31 to one grapevine, and tuf-b1/S1/V2-TA/Rqg50 to one periwinkle, indicating that D. europaea is an intermediate vector in CaPsol epidemiological cycles.",
publisher = "MDPI Basel",
journal = "Horticulturae",
title = "Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’",
number = "7",
pages = "654",
volume = "8",
doi = "10.3390/horticulturae8070654"
}

Cvrković, T., Jovic, J., Krstić, O., Marinković, S., Jakovljević, M., Mitrović, M.,& Tosevski, I.. (2022). Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’. in Horticulturae
MDPI Basel., 8(7), 654.
https://doi.org/10.3390/horticulturae8070654

Cvrković T, Jovic J, Krstić O, Marinković S, Jakovljević M, Mitrović M, Tosevski I. Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’. in Horticulturae. 2022;8(7):654.
doi:10.3390/horticulturae8070654 .

Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, "Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’" in Horticulturae, 8, no. 7 (2022):654,
https://doi.org/10.3390/horticulturae8070654 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/690
AB  - Diseases associated with ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) are of great economic importance, causing severe damage on a wide range of crops. Polyphagous insect vectors and wild plants acting as natural reservoir both play a major role in the epidemiology of phytoplasmas directly determining their transmission and persistence in agroecosystems. This is especially the case for stolbur phytoplasma diseases induced by ‘Ca. P. solani’ a pathogen native to Europe and with a plethora of natural reservoir plants and insect vectors, some being host specific and some being generalists. Fluctuating market demands shape farming in Serbia, often limiting production to narrow or repetitive crop rotation, providing favorable conditions for continuous re-appearance of stolbur phytoplasma epidemics. Our aim was to generate scenarios in which relevant biological factors (e.g. susceptibility of crops, natural reservoirs of phytoplasma, insect vectors and their host preferences), may threat crop fields by triggering re-emergence of stolbur epidemics. Our specific objective was to assess the potential risks and to define the best management practices to cope with disease outbreaks. The first step was building an easy-to-follow online free Compendium for farmers with the list of: a) all validated host plants for stolbur phytoplasma, including both cultivated and wild plants and, b) all known insect vectors and their hosts. The Compendium also serves as a platform for a risk assessment framework integrated with a decision-making scheme that proposes different options for land use architecture and crop allocation at diverse temporal and spatial scales.
PB  - Springer Verlag
T2  - Journal of Plant Pathology
T1  - Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia
EP  - 552
SP  - 537
VL  - 104
DO  - 10.1007/s42161-022-01055-9
ER  - 

@article{
author = "Mitrović, Milana and Marinković, Slavica and Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Jakovljević, Miljana",
year = "2022",
abstract = "Diseases associated with ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) are of great economic importance, causing severe damage on a wide range of crops. Polyphagous insect vectors and wild plants acting as natural reservoir both play a major role in the epidemiology of phytoplasmas directly determining their transmission and persistence in agroecosystems. This is especially the case for stolbur phytoplasma diseases induced by ‘Ca. P. solani’ a pathogen native to Europe and with a plethora of natural reservoir plants and insect vectors, some being host specific and some being generalists. Fluctuating market demands shape farming in Serbia, often limiting production to narrow or repetitive crop rotation, providing favorable conditions for continuous re-appearance of stolbur phytoplasma epidemics. Our aim was to generate scenarios in which relevant biological factors (e.g. susceptibility of crops, natural reservoirs of phytoplasma, insect vectors and their host preferences), may threat crop fields by triggering re-emergence of stolbur epidemics. Our specific objective was to assess the potential risks and to define the best management practices to cope with disease outbreaks. The first step was building an easy-to-follow online free Compendium for farmers with the list of: a) all validated host plants for stolbur phytoplasma, including both cultivated and wild plants and, b) all known insect vectors and their hosts. The Compendium also serves as a platform for a risk assessment framework integrated with a decision-making scheme that proposes different options for land use architecture and crop allocation at diverse temporal and spatial scales.",
publisher = "Springer Verlag",
journal = "Journal of Plant Pathology",
title = "Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia",
pages = "552-537",
volume = "104",
doi = "10.1007/s42161-022-01055-9"
}

Mitrović, M., Marinković, S., Cvrković, T., Jovic, J., Krstić, O.,& Jakovljević, M.. (2022). Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia. in Journal of Plant Pathology
Springer Verlag., 104, 537-552.
https://doi.org/10.1007/s42161-022-01055-9

Mitrović M, Marinković S, Cvrković T, Jovic J, Krstić O, Jakovljević M. Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia. in Journal of Plant Pathology. 2022;104:537-552.
doi:10.1007/s42161-022-01055-9 .

Mitrović, Milana, Marinković, Slavica, Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Jakovljević, Miljana, "Framework for risk assessment of ‘Candidatus Phytoplasma solani’associated diseases outbreaks in agroecosystems in Serbia" in Journal of Plant Pathology, 104 (2022):537-552,
https://doi.org/10.1007/s42161-022-01055-9 . .

TY  - CONF
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/815
PB  - Entomološko društvo Srbije
C3  - XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16
T1  - Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze
ER  - 

@conference{
author = "Jovic, Jelena and Krstić, Oliver and Cvrković, Tatjana and Jakovljević, Miljana and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
publisher = "Entomološko društvo Srbije",
journal = "XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16",
title = "Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze"
}

Jovic, J., Krstić, O., Cvrković, T., Jakovljević, M., Marinković, S., Mitrović, M.,& Tosevski, I.. (2022). Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze. in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16
Entomološko društvo Srbije..

Jovic J, Krstić O, Cvrković T, Jakovljević M, Marinković S, Mitrović M, Tosevski I. Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze. in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16. 2022;..

Jovic, Jelena, Krstić, Oliver, Cvrković, Tatjana, Jakovljević, Miljana, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, "Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze" in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16 (2022).

TY  - CONF
AU  - Jakovljević, Miljana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/794
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16
T1  - Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri
ER  - 

@conference{
author = "Jakovljević, Miljana and Tosevski, Ivo and Jovic, Jelena and Mitrović, Milana and Marinković, Slavica and Krstić, Oliver and Cvrković, Tatjana",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16",
title = "Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri"
}

Jakovljević, M., Tosevski, I., Jovic, J., Mitrović, M., Marinković, S., Krstić, O.,& Cvrković, T.. (2022). Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri. in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16
Entomological Society of Serbia..

Jakovljević M, Tosevski I, Jovic J, Mitrović M, Marinković S, Krstić O, Cvrković T. Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri. in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16. 2022;..

Jakovljević, Miljana, Tosevski, Ivo, Jovic, Jelena, Mitrović, Milana, Marinković, Slavica, Krstić, Oliver, Cvrković, Tatjana, "Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri" in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16 (2022).

TY  - CONF
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/786
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot
T1  - Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme
ER  - 

@conference{
author = "Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot",
title = "Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme"
}

Cvrković, T., Jovic, J., Krstić, O., Marinković, S., Jakovljević, M., Mitrović, M.,& Tosevski, I.. (2022). Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme. in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot
Entomological Society of Serbia..

Cvrković T, Jovic J, Krstić O, Marinković S, Jakovljević M, Mitrović M, Tosevski I. Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme. in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot. 2022;..

Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, "Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme" in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot (2022).

TY  - CONF
AU  - Marinković, Slavica
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/777
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.
T1  - Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji
ER  - 

@conference{
author = "Marinković, Slavica and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Jovic, Jelena and Tosevski, Ivo",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.",
title = "Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji"
}

Marinković, S., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Jovic, J.,& Tosevski, I.. (2022). Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji. in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.
Entomological Society of Serbia..

Marinković S, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Jovic J, Tosevski I. Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji. in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.. 2022;..

Marinković, Slavica, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Jovic, Jelena, Tosevski, Ivo, "Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji" in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022. (2022).

TY  - JOUR
AU  - Jovic, Jelena
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/693
AB  - Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs
of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse
agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely
related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that
is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms
association of the two phytoplasmas in shared host plants were the subject of this study. Specific
primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification
of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous
detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among
symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca.
P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas.
None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants
from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected
solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in
plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35)
were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’.
Variations in symptoms and their association with each of the phytoplasmas are described and
documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’
infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific
alterations in infected bindweeds. The results are discussed in the context of the epidemiological
and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two
phytoplasma relatives in shared host plant.
PB  - MDPI Basel
T2  - Pathogens
T1  - Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed
IS  - 2
SP  - 160
VL  - 10
DO  - 10.3390/pathogens10020160
ER  - 

@article{
author = "Jovic, Jelena and Marinković, Slavica and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana",
year = "2021",
abstract = "Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs
of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse
agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely
related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that
is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms
association of the two phytoplasmas in shared host plants were the subject of this study. Specific
primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification
of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous
detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among
symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca.
P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas.
None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants
from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected
solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in
plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35)
were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’.
Variations in symptoms and their association with each of the phytoplasmas are described and
documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’
infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific
alterations in infected bindweeds. The results are discussed in the context of the epidemiological
and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two
phytoplasma relatives in shared host plant.",
publisher = "MDPI Basel",
journal = "Pathogens",
title = "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed",
number = "2",
pages = "160",
volume = "10",
doi = "10.3390/pathogens10020160"
}

Jovic, J., Marinković, S., Jakovljević, M., Krstić, O., Cvrković, T.,& Mitrović, M.. (2021). Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens
MDPI Basel., 10(2), 160.
https://doi.org/10.3390/pathogens10020160

Jovic J, Marinković S, Jakovljević M, Krstić O, Cvrković T, Mitrović M. Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens. 2021;10(2):160.
doi:10.3390/pathogens10020160 .

Jovic, Jelena, Marinković, Slavica, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed" in Pathogens, 10, no. 2 (2021):160,
https://doi.org/10.3390/pathogens10020160 . .

TY  - JOUR
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/664
AB  - Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae), known as the mosaic leafhopper, is an invasive alien species native to the Eastern Palaearctic, but also widespread and well established on the eastern territory of North America. Since its first detection in Europe in 1998, this polyphagous species has expanded its distribution area and rapidly spread through central and western European countries, inhabiting a wide range of broadleaf trees and shrubs (e.g. Gleditsia triacanthos, Salix spp., Corylus spp., Acer spp., Betula spp., Populus spp. and Carpinus spp.), which are usually present in the surrounding of vineyards. Over the last decade, O. ishidae was frequently found within European vineyards and was proven to be able to complete its life cycle on grapevine. Moreover, this leafhopper was shown to be naturally infected with the Flavescence dorée phytoplasma (FDp), a devastating disease which causes significant economic losses in the major vine-producing countries. This study provides data on the occurrence of O. ishidae in Serbia, clearly confirming that it has expanded its distribution range to the Balkan Peninsula which could lead to establishment of new FDp epidemiological cycles inside the local vineyard regions. As a consequence, there could be an increased negative impact on phytosanitary situation with a continual spread of this vector species and FDp epidemic outbreaks in Southeastern Europe.
PB  - Regional Euro-Asian Biological Invasions Centre
T2  - BioInvasions Records
T1  - The “code red” for Balkan vineyards: occurrence of Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae) in Serbia
EP  - 554
IS  - 3
SP  - 544
VL  - 10
DO  - 10.3391/BIR.2021.10.3.04
ER  - 

@article{
author = "Cvrković, Tatjana and Jovic, Jelena and Jakovljević, Miljana and Krstić, Oliver and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo",
year = "2021",
abstract = "Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae), known as the mosaic leafhopper, is an invasive alien species native to the Eastern Palaearctic, but also widespread and well established on the eastern territory of North America. Since its first detection in Europe in 1998, this polyphagous species has expanded its distribution area and rapidly spread through central and western European countries, inhabiting a wide range of broadleaf trees and shrubs (e.g. Gleditsia triacanthos, Salix spp., Corylus spp., Acer spp., Betula spp., Populus spp. and Carpinus spp.), which are usually present in the surrounding of vineyards. Over the last decade, O. ishidae was frequently found within European vineyards and was proven to be able to complete its life cycle on grapevine. Moreover, this leafhopper was shown to be naturally infected with the Flavescence dorée phytoplasma (FDp), a devastating disease which causes significant economic losses in the major vine-producing countries. This study provides data on the occurrence of O. ishidae in Serbia, clearly confirming that it has expanded its distribution range to the Balkan Peninsula which could lead to establishment of new FDp epidemiological cycles inside the local vineyard regions. As a consequence, there could be an increased negative impact on phytosanitary situation with a continual spread of this vector species and FDp epidemic outbreaks in Southeastern Europe.",
publisher = "Regional Euro-Asian Biological Invasions Centre",
journal = "BioInvasions Records",
title = "The “code red” for Balkan vineyards: occurrence of Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae) in Serbia",
pages = "554-544",
number = "3",
volume = "10",
doi = "10.3391/BIR.2021.10.3.04"
}

Cvrković, T., Jovic, J., Jakovljević, M., Krstić, O., Marinković, S., Mitrović, M.,& Tosevski, I.. (2021). The “code red” for Balkan vineyards: occurrence of Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae) in Serbia. in BioInvasions Records
Regional Euro-Asian Biological Invasions Centre., 10(3), 544-554.
https://doi.org/10.3391/BIR.2021.10.3.04

Cvrković T, Jovic J, Jakovljević M, Krstić O, Marinković S, Mitrović M, Tosevski I. The “code red” for Balkan vineyards: occurrence of Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae) in Serbia. in BioInvasions Records. 2021;10(3):544-554.
doi:10.3391/BIR.2021.10.3.04 .

Cvrković, Tatjana, Jovic, Jelena, Jakovljević, Miljana, Krstić, Oliver, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, "The “code red” for Balkan vineyards: occurrence of Orientus ishidae (Matsumura, 1902) (Hemiptera: Cicadellidae) in Serbia" in BioInvasions Records, 10, no. 3 (2021):544-554,
https://doi.org/10.3391/BIR.2021.10.3.04 . .

TY  - JOUR
AU  - Malembic-Maher, Sylvie
AU  - Desque, Delphine
AU  - Khalil, Dima
AU  - Salar, Pascal
AU  - Bergey, Bernard
AU  - Danet, Jean-Luc
AU  - Duret, Sybille
AU  - Dubrana-Ourabah, Marie-Pierre
AU  - Beven, Laure
AU  - Ember, Ibolyia
AU  - Acs, Zoltan
AU  - Della Bartola, Michele
AU  - MATERAZZI, ALBERTO
AU  - Fillipin, Luisa
AU  - Krnjajić, Slobodan
AU  - Krstić, Oliver
AU  - Tosevski, Ivo
AU  - Lang, Friederike
AU  - Jarausch, Barbara
AU  - Kolber, Maria
AU  - Jovic, Jelena
AU  - angelini, elisa
AU  - Arricau-Bouvery, Nathalie
AU  - Maixner, Michael
AU  - Foissac, Xavier
PY  - 2020-03-25
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/644
AB  - Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and–III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties.
PB  - Public Library of Science
T2  - PLOS PATHOGENS
T1  - When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe
IS  - 3
VL  - 16
DO  - 10.1371/journal.ppat.1007967
ER  - 

@article{
author = "Malembic-Maher, Sylvie and Desque, Delphine and Khalil, Dima and Salar, Pascal and Bergey, Bernard and Danet, Jean-Luc and Duret, Sybille and Dubrana-Ourabah, Marie-Pierre and Beven, Laure and Ember, Ibolyia and Acs, Zoltan and Della Bartola, Michele and MATERAZZI, ALBERTO and Fillipin, Luisa and Krnjajić, Slobodan and Krstić, Oliver and Tosevski, Ivo and Lang, Friederike and Jarausch, Barbara and Kolber, Maria and Jovic, Jelena and angelini, elisa and Arricau-Bouvery, Nathalie and Maixner, Michael and Foissac, Xavier",
year = "2020-03-25",
abstract = "Flavescence dorée (FD) is a European quarantine grapevine disease transmitted by the Deltocephalinae leafhopper Scaphoideus titanus. Whereas this vector had been introduced from North America, the possible European origin of FD phytoplasma needed to be challenged and correlated with ecological and genetic drivers of FD emergence. For that purpose, a survey of genetic diversity of these phytoplasmas in grapevines, S. titanus, black alders, alder leafhoppers and clematis were conducted in five European countries. Out of 132 map genotypes, only 11 were associated to FD outbreaks, three were detected in clematis, whereas 127 were detected in alder trees, alder leafhoppers or in grapevines out of FD outbreaks. Most of the alder trees were found infected, including 8% with FD genotypes M6, M38 and M50, also present in alders neighboring FD-free vineyards and vineyard-free areas. The Macropsinae Oncopsis alni could transmit genotypes unable to achieve transmission by S. titanus, while the Deltocephalinae Allygus spp. and Orientus ishidae transmitted M38 and M50 that proved to be compatible with S. titanus. Variability of vmpA and vmpB adhesin-like genes clearly discriminated 3 genetic clusters. Cluster Vmp-I grouped genotypes only transmitted by O. alni, while clusters Vmp-II and -III grouped genotypes transmitted by Deltocephalinae leafhoppers. Interestingly, adhesin repeated domains evolved independently in cluster Vmp-I, whereas in clusters Vmp-II and–III showed recent duplications. Latex beads coated with various ratio of VmpA of clusters II and I, showed that cluster II VmpA promoted enhanced adhesion to the Deltocephalinae Euscelidius variegatus epithelial cells and were better retained in both E. variegatus and S. titanus midguts. Our data demonstrate that most FD phytoplasmas are endemic to European alders. Their emergence as grapevine epidemic pathogens appeared restricted to some genetic variants pre-existing in alders, whose compatibility to S. titanus correlates with different vmp gene sequences and VmpA binding properties.",
publisher = "Public Library of Science",
journal = "PLOS PATHOGENS",
title = "When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe",
number = "3",
volume = "16",
doi = "10.1371/journal.ppat.1007967"
}

Malembic-Maher, S., Desque, D., Khalil, D., Salar, P., Bergey, B., Danet, J., Duret, S., Dubrana-Ourabah, M., Beven, L., Ember, I., Acs, Z., Della Bartola, M., MATERAZZI, A., Fillipin, L., Krnjajić, S., Krstić, O., Tosevski, I., Lang, F., Jarausch, B., Kolber, M., Jovic, J., angelini, e., Arricau-Bouvery, N., Maixner, M.,& Foissac, X.. (2020-03-25). When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe. in PLOS PATHOGENS
Public Library of Science., 16(3).
https://doi.org/10.1371/journal.ppat.1007967

Malembic-Maher S, Desque D, Khalil D, Salar P, Bergey B, Danet J, Duret S, Dubrana-Ourabah M, Beven L, Ember I, Acs Z, Della Bartola M, MATERAZZI A, Fillipin L, Krnjajić S, Krstić O, Tosevski I, Lang F, Jarausch B, Kolber M, Jovic J, angelini E, Arricau-Bouvery N, Maixner M, Foissac X. When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe. in PLOS PATHOGENS. 2020;16(3).
doi:10.1371/journal.ppat.1007967 .

Malembic-Maher, Sylvie, Desque, Delphine, Khalil, Dima, Salar, Pascal, Bergey, Bernard, Danet, Jean-Luc, Duret, Sybille, Dubrana-Ourabah, Marie-Pierre, Beven, Laure, Ember, Ibolyia, Acs, Zoltan, Della Bartola, Michele, MATERAZZI, ALBERTO, Fillipin, Luisa, Krnjajić, Slobodan, Krstić, Oliver, Tosevski, Ivo, Lang, Friederike, Jarausch, Barbara, Kolber, Maria, Jovic, Jelena, angelini, elisa, Arricau-Bouvery, Nathalie, Maixner, Michael, Foissac, Xavier, "When a Palearctic bacterium meets a Nearctic insect vector: Genetic and ecological insights into the emergence of the grapevine Flavescence dore´e epidemics in Europe" in PLOS PATHOGENS, 16, no. 3 (2020-03-25),
https://doi.org/10.1371/journal.ppat.1007967 . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/588
AB  - Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.
PB  - Springer Nature
T2  - European Journal of Plant Pathology
T1  - Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential
EP  - 221
SP  - 201
VL  - 156
DO  - 10.1007/s10658-019-01878-w
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Mitrović, Milana and Marinković, Slavica and Toševski, Ivo and Cvrković, Tatjana",
year = "2020",
abstract = "Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.",
publisher = "Springer Nature",
journal = "European Journal of Plant Pathology",
title = "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential",
pages = "221-201",
volume = "156",
doi = "10.1007/s10658-019-01878-w"
}

Jakovljević, M., Jović, J., Krstić, O., Mitrović, M., Marinković, S., Toševski, I.,& Cvrković, T.. (2020). Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology
Springer Nature., 156, 201-221.
https://doi.org/10.1007/s10658-019-01878-w

Jakovljević M, Jović J, Krstić O, Mitrović M, Marinković S, Toševski I, Cvrković T. Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology. 2020;156:201-221.
doi:10.1007/s10658-019-01878-w .

Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Mitrović, Milana, Marinković, Slavica, Toševski, Ivo, Cvrković, Tatjana, "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential" in European Journal of Plant Pathology, 156 (2020):201-221,
https://doi.org/10.1007/s10658-019-01878-w . .

TY  - CONF
AU  - Mitrović, Milana
AU  - Trivellone, Valeria
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1016
AB  - Print ISSN : 2249-4669. Online ISSN : 2249-4677.




Indian journals 
DIVA ENTERPRISES PVT. LTD..
The vector status of the deltocephalinae leafhopper Neoaliturus fenestratus harbouring ‘Candidatus Phytoplasma solani’ (“stolbur”) was tested in two separate transmission trials with seedlings of lettuce and carrot. In both tests, the leafhopper successfully transmitted the “stolbur” phytoplasma to the exposed plants, which expressed partial leaf discoloration and rolling and rapid plant desiccation. Molecular characterization of the stamp gene encoding the antigenic membrane protein determined the presence of three “stolbur” phytoplasma strains in field collected N. fenestratus and in the insect inoculated plants. The same genotypes of phytoplasma shared by the vector samples and the exposed lettuce and carrot seedlings confirm the ability of N. fenestratus to transmit the “stolbur” phytoplasma to the tested plants. These findings indicate a possible role of N. fenestratus in the epidemiology of the “stolbur” phytoplasma in vegetable crops.
PB  - Indian journals Diva Enterprises PVT
C3  - Phytopathogenic Mollicutes
T1  - Experimental and molecular evidence of Neoaliturus fenestratus role in the transmission of “stolbur” phytoplasma to lettuce and carrot plants
EP  - 110
IS  - 1
SP  - 109
VL  - 9
ER  - 

@conference{
author = "Mitrović, Milana and Trivellone, Valeria and Cvrković, Tatjana and Jakovljević, Miljana and Krstić, Oliver and Jovic, Jelena and Tosevski, Ivo",
year = "2019",
abstract = "Print ISSN : 2249-4669. Online ISSN : 2249-4677.




Indian journals 
DIVA ENTERPRISES PVT. LTD..
The vector status of the deltocephalinae leafhopper Neoaliturus fenestratus harbouring ‘Candidatus Phytoplasma solani’ (“stolbur”) was tested in two separate transmission trials with seedlings of lettuce and carrot. In both tests, the leafhopper successfully transmitted the “stolbur” phytoplasma to the exposed plants, which expressed partial leaf discoloration and rolling and rapid plant desiccation. Molecular characterization of the stamp gene encoding the antigenic membrane protein determined the presence of three “stolbur” phytoplasma strains in field collected N. fenestratus and in the insect inoculated plants. The same genotypes of phytoplasma shared by the vector samples and the exposed lettuce and carrot seedlings confirm the ability of N. fenestratus to transmit the “stolbur” phytoplasma to the tested plants. These findings indicate a possible role of N. fenestratus in the epidemiology of the “stolbur” phytoplasma in vegetable crops.",
publisher = "Indian journals Diva Enterprises PVT",
journal = "Phytopathogenic Mollicutes",
title = "Experimental and molecular evidence of Neoaliturus fenestratus role in the transmission of “stolbur” phytoplasma to lettuce and carrot plants",
pages = "110-109",
number = "1",
volume = "9"
}

Mitrović, M., Trivellone, V., Cvrković, T., Jakovljević, M., Krstić, O., Jovic, J.,& Tosevski, I.. (2019). Experimental and molecular evidence of Neoaliturus fenestratus role in the transmission of “stolbur” phytoplasma to lettuce and carrot plants. in Phytopathogenic Mollicutes
Indian journals Diva Enterprises PVT., 9(1), 109-110.

Mitrović M, Trivellone V, Cvrković T, Jakovljević M, Krstić O, Jovic J, Tosevski I. Experimental and molecular evidence of Neoaliturus fenestratus role in the transmission of “stolbur” phytoplasma to lettuce and carrot plants. in Phytopathogenic Mollicutes. 2019;9(1):109-110..

Mitrović, Milana, Trivellone, Valeria, Cvrković, Tatjana, Jakovljević, Miljana, Krstić, Oliver, Jovic, Jelena, Tosevski, Ivo, "Experimental and molecular evidence of Neoaliturus fenestratus role in the transmission of “stolbur” phytoplasma to lettuce and carrot plants" in Phytopathogenic Mollicutes, 9, no. 1 (2019):109-110.

TY  - CONF
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/984
AB  - Surveys for the presence of symptoms typical for the “stolbur” disease were conducted during 2015 and 2016 in pepper growing areas of Serbia. ‘Candidatus Phytoplasma solani’ detection was performed by Stol11 primer amplification. Characterisation was done by multi-locus typing (MLST) of tuf, secY, vmp1 and stamp genes. The disease was observed in pepper in all the surveyed locations except in south Serbia, whith a low incidence (3-5%). Overall, 13 MLST genotypes were distinguished, and the most common were Rqg31/Rqg50, Rpm35/M5 and STOL.
PB  - Technology Society of Basic and Applied Sciences
C3  - Phytopathogenic Mollicutes
T1  - High genetic diversity of ‘Candidatus phytoplasma solani’ infecting pepper in Serbia
EP  - 38
IS  - 1
IS  - 37
VL  - 9
DO  - 10.5958/2249-4677.2019.00019.7
ER  - 

@conference{
author = "Jovic, Jelena and Tosevski, Ivo and Krstić, Oliver and Jakovljević, Miljana and Kosovac, Andrea and Cvrković, Tatjana and Mitrović, Milana",
year = "2019",
abstract = "Surveys for the presence of symptoms typical for the “stolbur” disease were conducted during 2015 and 2016 in pepper growing areas of Serbia. ‘Candidatus Phytoplasma solani’ detection was performed by Stol11 primer amplification. Characterisation was done by multi-locus typing (MLST) of tuf, secY, vmp1 and stamp genes. The disease was observed in pepper in all the surveyed locations except in south Serbia, whith a low incidence (3-5%). Overall, 13 MLST genotypes were distinguished, and the most common were Rqg31/Rqg50, Rpm35/M5 and STOL.",
publisher = "Technology Society of Basic and Applied Sciences",
journal = "Phytopathogenic Mollicutes",
title = "High genetic diversity of ‘Candidatus phytoplasma solani’ infecting pepper in Serbia",
pages = "38",
number = "1, 37",
volume = "9",
doi = "10.5958/2249-4677.2019.00019.7"
}

Jovic, J., Tosevski, I., Krstić, O., Jakovljević, M., Kosovac, A., Cvrković, T.,& Mitrović, M.. (2019). High genetic diversity of ‘Candidatus phytoplasma solani’ infecting pepper in Serbia. in Phytopathogenic Mollicutes
Technology Society of Basic and Applied Sciences., 9(1).
https://doi.org/10.5958/2249-4677.2019.00019.7

Jovic J, Tosevski I, Krstić O, Jakovljević M, Kosovac A, Cvrković T, Mitrović M. High genetic diversity of ‘Candidatus phytoplasma solani’ infecting pepper in Serbia. in Phytopathogenic Mollicutes. 2019;9(1):null-38.
doi:10.5958/2249-4677.2019.00019.7 .

Jovic, Jelena, Tosevski, Ivo, Krstić, Oliver, Jakovljević, Miljana, Kosovac, Andrea, Cvrković, Tatjana, Mitrović, Milana, "High genetic diversity of ‘Candidatus phytoplasma solani’ infecting pepper in Serbia" in Phytopathogenic Mollicutes, 9, no. 1 (2019),
https://doi.org/10.5958/2249-4677.2019.00019.7 . .

TY  - JOUR
AU  - Sedlarević-Zorić, Ana
AU  - Morina, Filis
AU  - Toševski, Ivo
AU  - Tosti, Tomislav
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/569
AB  - Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.
PB  - Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales
T2  - Plant Physiology and Biochemistry
T1  - Resource allocation in response to herbivory and gall formation in Linaria vulgaris
EP  - 232
SP  - 224
VL  - 135
DO  - 10.1016/j.plaphy.2018.11.032
ER  - 

@article{
author = "Sedlarević-Zorić, Ana and Morina, Filis and Toševski, Ivo and Tosti, Tomislav and Jović, Jelena and Krstić, Oliver and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.",
publisher = "Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales",
journal = "Plant Physiology and Biochemistry",
title = "Resource allocation in response to herbivory and gall formation in Linaria vulgaris",
pages = "232-224",
volume = "135",
doi = "10.1016/j.plaphy.2018.11.032"
}

Sedlarević-Zorić, A., Morina, F., Toševski, I., Tosti, T., Jović, J., Krstić, O.,& Veljović-Jovanović, S.. (2019). Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry
Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales., 135, 224-232.
https://doi.org/10.1016/j.plaphy.2018.11.032

Sedlarević-Zorić A, Morina F, Toševski I, Tosti T, Jović J, Krstić O, Veljović-Jovanović S. Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry. 2019;135:224-232.
doi:10.1016/j.plaphy.2018.11.032 .

Sedlarević-Zorić, Ana, Morina, Filis, Toševski, Ivo, Tosti, Tomislav, Jović, Jelena, Krstić, Oliver, Veljović-Jovanović, Sonja, "Resource allocation in response to herbivory and gall formation in Linaria vulgaris" in Plant Physiology and Biochemistry, 135 (2019):224-232,
https://doi.org/10.1016/j.plaphy.2018.11.032 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/565
AB  - Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia
EP  - 195
IS  - 1
SP  - 183
VL  - 153
DO  - 10.1007/s10658-018-1553-1
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2019",
abstract = "Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia",
pages = "195-183",
number = "1",
volume = "153",
doi = "10.1007/s10658-018-1553-1"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2019). Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 153(1), 183-195.
https://doi.org/10.1007/s10658-018-1553-1

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology. 2019;153(1):183-195.
doi:10.1007/s10658-018-1553-1 .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia" in European Journal of Plant Pathology, 153, no. 1 (2019):183-195,
https://doi.org/10.1007/s10658-018-1553-1 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/547
AB  - The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).
PB  - Public Library of Science (PLoS)
T2  - PlOS One
T1  - Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation
IS  - 5
VL  - 13
DO  - 10.1371/journal.pone.0196969
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).",
publisher = "Public Library of Science (PLoS)",
journal = "PlOS One",
title = "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation",
number = "5",
volume = "13",
doi = "10.1371/journal.pone.0196969"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2018). Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One
Public Library of Science (PLoS)., 13(5).
https://doi.org/10.1371/journal.pone.0196969

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One. 2018;13(5).
doi:10.1371/journal.pone.0196969 .

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation" in PlOS One, 13, no. 5 (2018),
https://doi.org/10.1371/journal.pone.0196969 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/550
AB  - The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions
EP  - 64
IS  - 1
SP  - 47
VL  - 172
DO  - 10.1111/aab.12400
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Radonjić, Sanja and Hrnčić, Snježana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions",
pages = "64-47",
number = "1",
volume = "172",
doi = "10.1111/aab.12400"
}

Krstić, O., Cvrković, T., Mitrović, M., Radonjić, S., Hrnčić, S., Toševski, I.,& Jović, J.. (2018). Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology
Wiley, Hoboken., 172(1), 47-64.
https://doi.org/10.1111/aab.12400

Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J. Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology. 2018;172(1):47-64.
doi:10.1111/aab.12400 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Radonjić, Sanja, Hrnčić, Snježana, Toševski, Ivo, Jović, Jelena, "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions" in Annals of Applied Biology, 172, no. 1 (2018):47-64,
https://doi.org/10.1111/aab.12400 . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Sing, Sharlene
AU  - De Clerck-Floate, Rosemarie
AU  - McClay, A.
AU  - Weaver, D. K.
AU  - Schwarzlander, M.
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Gassmann, Andre
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/549
AB  - Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America
EP  - 34
IS  - 1
SP  - 16
VL  - 173
DO  - 10.1111/aab.12430
ER  - 

@article{
author = "Toševski, Ivo and Sing, Sharlene and De Clerck-Floate, Rosemarie and McClay, A. and Weaver, D. K. and Schwarzlander, M. and Krstić, Oliver and Jović, Jelena and Gassmann, Andre",
year = "2018",
abstract = "Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America",
pages = "34-16",
number = "1",
volume = "173",
doi = "10.1111/aab.12430"
}

Toševski, I., Sing, S., De Clerck-Floate, R., McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, O., Jović, J.,& Gassmann, A.. (2018). Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology
Wiley, Hoboken., 173(1), 16-34.
https://doi.org/10.1111/aab.12430

Toševski I, Sing S, De Clerck-Floate R, McClay A, Weaver DK, Schwarzlander M, Krstić O, Jović J, Gassmann A. Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology. 2018;173(1):16-34.
doi:10.1111/aab.12430 .

Toševski, Ivo, Sing, Sharlene, De Clerck-Floate, Rosemarie, McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, Oliver, Jović, Jelena, Gassmann, Andre, "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America" in Annals of Applied Biology, 173, no. 1 (2018):16-34,
https://doi.org/10.1111/aab.12430 . .

TY  - THES
AU  - Krstić, Oliver
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5504
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16897/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025175218
UR  - http://nardus.mpn.gov.rs/123456789/9040
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/573
AB  - Dictyophara europaea je široko rasprostranjena i ekonomski značajna cikada koja kaoalternativni vektor Flavescence dorée (FD) fitoplazme, učestvuje u prenošenju bolesti uvinogradima Evrope. Sprovedena epidemiološka istraživanja su ukazala na postojanjepopulacija D. europaea sa veoma visokom stopom FD-infekcije, Wolbachia-inficiranihpopulacija sa niskom stopom FD-infekcije (DeWo+) i odsustvo Wolbachia upopulacijama sa visokom stopom FD-infekcije (DeWo-). Ispitivano je nekolikomogućih uzroka raličite stope infekcije vektora: i) populaciono-genetičke karakteristikeD. europaea i korelacija sa Wolbachia infekcijom, ii) efekat Wolbachia na komponenteadaptivne vrednosti laboratorijske kolonije DeWo+, i iii) stopa inficiranosti prirodnogbiljnog rezervoara FD fitoplazme i razlike u genotipovima fitoplazme u nisko- i visokoinficiranimpopulacijama. Genotipizacijom mitohondrijskog COI gena evidentiran jesmanjen genetički diverzitet DeWo+ u poređenju sa DeWo- populacijama i različitaevolucija fiksiranih haplotipova. Multilokusnom genotipizacijom Wolbachia izolataidentifikovan je isti soj, wEur, u svim DeWo+ populacijama. Diverzitet FD fitoplazmenije bio u korelaciji sa različitom stopom inficiranosti vektora. Utvrđeno je daWolbachia-infekcija nema direktnog efekta na adaptivnu vrednost D. europaea. Podaciiz prirodnih populacija D. europaea ukazuju na kompeticiju Wolbachia i FD fitoplazme:populacije koje su u niskoj stopi inficirane FD fitoplazmom su Wolbachia-inficirane ivice versa.
AB  - Dictyophara europaea is widespread planthopper of economic importance, acting as analternative vector of the Flavescence dorée (FD) phytoplasma disease of grapevine inEuropean vineyards. Epidemiological studies have revealed D. europaea populationsthat are heavily FD-infected, as well as populations with low FD-infection rates that arenaturally infected with Wolbachia (DeWo+), and highly FD-infected populations in theabsence of Wolbachia (DeWo-).Several possible causes of differences in vectorinfection rates were examined: i) population genetic characteristics of D. europaea andtheir correlation with Wolbachia infection, ii) Wolbachia effects on fitness componentsof DeWo+ laboratory colony, and iii) the rate of reservoir plant FD-infection anddifferences in FD genotypes harboured by low and high infected vector populations.Genotyping of mitochondrial COI loci showed lower genetic diversity level in DeWo+than in DeWo- populations of and divergent evolution of fixed haplotypes. Multilocusgenotyping of Wolbachia revealed that all DeWo+ populations were infected with thesame strain (i.e. wEur). Genetically diversified FD phytoplasma had no direct influenceon vector infection rates. No evidence of direct fitness effects on D. europaea wasregistered with regard to Wolbachia infection status. Field data and the observednegative correlation between FD-infection and Wolbachia-infection rates, indicate thatWolbachia compete with FD phytoplasma within the host insect D. europaea.
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Uloga evolucionih interakcija između intracelularnog endosimbionta (Wolbachia) i fitoplazme (Flavescence dorée) u promenama komponenti adaptivne vrednosti i pravcima evolucije mitohondrijske DNK u prirodnim populacijama Dictyophara europaea
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9040
ER  - 

@phdthesis{
author = "Krstić, Oliver",
year = "2017",
abstract = "Dictyophara europaea je široko rasprostranjena i ekonomski značajna cikada koja kaoalternativni vektor Flavescence dorée (FD) fitoplazme, učestvuje u prenošenju bolesti uvinogradima Evrope. Sprovedena epidemiološka istraživanja su ukazala na postojanjepopulacija D. europaea sa veoma visokom stopom FD-infekcije, Wolbachia-inficiranihpopulacija sa niskom stopom FD-infekcije (DeWo+) i odsustvo Wolbachia upopulacijama sa visokom stopom FD-infekcije (DeWo-). Ispitivano je nekolikomogućih uzroka raličite stope infekcije vektora: i) populaciono-genetičke karakteristikeD. europaea i korelacija sa Wolbachia infekcijom, ii) efekat Wolbachia na komponenteadaptivne vrednosti laboratorijske kolonije DeWo+, i iii) stopa inficiranosti prirodnogbiljnog rezervoara FD fitoplazme i razlike u genotipovima fitoplazme u nisko- i visokoinficiranimpopulacijama. Genotipizacijom mitohondrijskog COI gena evidentiran jesmanjen genetički diverzitet DeWo+ u poređenju sa DeWo- populacijama i različitaevolucija fiksiranih haplotipova. Multilokusnom genotipizacijom Wolbachia izolataidentifikovan je isti soj, wEur, u svim DeWo+ populacijama. Diverzitet FD fitoplazmenije bio u korelaciji sa različitom stopom inficiranosti vektora. Utvrđeno je daWolbachia-infekcija nema direktnog efekta na adaptivnu vrednost D. europaea. Podaciiz prirodnih populacija D. europaea ukazuju na kompeticiju Wolbachia i FD fitoplazme:populacije koje su u niskoj stopi inficirane FD fitoplazmom su Wolbachia-inficirane ivice versa., Dictyophara europaea is widespread planthopper of economic importance, acting as analternative vector of the Flavescence dorée (FD) phytoplasma disease of grapevine inEuropean vineyards. Epidemiological studies have revealed D. europaea populationsthat are heavily FD-infected, as well as populations with low FD-infection rates that arenaturally infected with Wolbachia (DeWo+), and highly FD-infected populations in theabsence of Wolbachia (DeWo-).Several possible causes of differences in vectorinfection rates were examined: i) population genetic characteristics of D. europaea andtheir correlation with Wolbachia infection, ii) Wolbachia effects on fitness componentsof DeWo+ laboratory colony, and iii) the rate of reservoir plant FD-infection anddifferences in FD genotypes harboured by low and high infected vector populations.Genotyping of mitochondrial COI loci showed lower genetic diversity level in DeWo+than in DeWo- populations of and divergent evolution of fixed haplotypes. Multilocusgenotyping of Wolbachia revealed that all DeWo+ populations were infected with thesame strain (i.e. wEur). Genetically diversified FD phytoplasma had no direct influenceon vector infection rates. No evidence of direct fitness effects on D. europaea wasregistered with regard to Wolbachia infection status. Field data and the observednegative correlation between FD-infection and Wolbachia-infection rates, indicate thatWolbachia compete with FD phytoplasma within the host insect D. europaea.",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Uloga evolucionih interakcija između intracelularnog endosimbionta (Wolbachia) i fitoplazme (Flavescence dorée) u promenama komponenti adaptivne vrednosti i pravcima evolucije mitohondrijske DNK u prirodnim populacijama Dictyophara europaea",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9040"
}

Krstić, O.. (2017). Uloga evolucionih interakcija između intracelularnog endosimbionta (Wolbachia) i fitoplazme (Flavescence dorée) u promenama komponenti adaptivne vrednosti i pravcima evolucije mitohondrijske DNK u prirodnim populacijama Dictyophara europaea. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9040

Krstić O. Uloga evolucionih interakcija između intracelularnog endosimbionta (Wolbachia) i fitoplazme (Flavescence dorée) u promenama komponenti adaptivne vrednosti i pravcima evolucije mitohondrijske DNK u prirodnim populacijama Dictyophara europaea. in Универзитет у Београду. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_9040 .

Krstić, Oliver, "Uloga evolucionih interakcija između intracelularnog endosimbionta (Wolbachia) i fitoplazme (Flavescence dorée) u promenama komponenti adaptivne vrednosti i pravcima evolucije mitohondrijske DNK u prirodnim populacijama Dictyophara europaea" in Универзитет у Београду (2017),
https://hdl.handle.net/21.15107/rcub_nardus_9040 .