TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/643
AB  - Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia
PB  - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
T2  - Spanish Journal of Agricultural Research
T1  - Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes
IS  - 4
VL  - 18
DO  - 10.5424/sjar/2020184-16244
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Lela and Vučurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia",
publisher = "Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)",
journal = "Spanish Journal of Agricultural Research",
title = "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes",
number = "4",
volume = "18",
doi = "10.5424/sjar/2020184-16244"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, L., Vučurović, I.,& Krstić, B.. (2020). Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)., 18(4).
https://doi.org/10.5424/sjar/2020184-16244

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić L, Vučurović I, Krstić B. Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research. 2020;18(4).
doi:10.5424/sjar/2020184-16244 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Lela, Vučurović, Ivan, Krstić, Branka, "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes" in Spanish Journal of Agricultural Research, 18, no. 4 (2020),
https://doi.org/10.5424/sjar/2020184-16244 . .