TY  - JOUR
AU  - Oro, Violeta
AU  - Knezevic, Magdalena
AU  - Dinić, Zoran
AU  - Delić, Dušica
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/751
AB  - The potato cyst nematode (PCN) Globodera rostochiensis is a plant parasite of potato classified into a group of quarantine organisms causing high economic losses worldwide. Due to the long persistence of the parasite in soil, cysts harbor numerous bacteria whose presence can lead to cyst death and population decline. The cysts of G. rostochiensis found in two potato fields were used as a source of bacteria. The universal procedure was applied to extract DNA from bacteria which was then sequenced with 16S primers. The aims of the study were to identify bacterial microbiota associated with the PCN populations and to infer their phylogenetic relationships based on the maximum likelihood and Bayesian phylogeny of the 16S sequences. In addition, the impact of the most significant climate and edaphic factors on bacterial diversity were evaluated. Regarding the higher taxonomy, our results indicate that the prevalent bacterial classes were Bacilli, Actinobacteria and Alphaproteobacteria. Phylogenetic analyses clustered Brevibacterium frigoritolerans within the family Bacillaceae, confirming its recent reclassification. Long-term climate factors, such as air temperature, insolation hours, humidity and precipitation, as well as the content of soil organic matter, affected the bacterial diversity. The ability of cyst nematodes to persist in soil for a long time qualifies them as a significant natural source to explore the soil bacterial microbiota.
PB  - MDPI
T2  - Plants
T1  - Bacterial microbiota isolated from cysts of Globodera rostochiensis (Nematoda: Heteroderidae).
IS  - 9
SP  - 1146
VL  - 9
DO  - 10.3390%2Fplants9091146
ER  - 

@article{
author = "Oro, Violeta and Knezevic, Magdalena and Dinić, Zoran and Delić, Dušica",
year = "2020",
abstract = "The potato cyst nematode (PCN) Globodera rostochiensis is a plant parasite of potato classified into a group of quarantine organisms causing high economic losses worldwide. Due to the long persistence of the parasite in soil, cysts harbor numerous bacteria whose presence can lead to cyst death and population decline. The cysts of G. rostochiensis found in two potato fields were used as a source of bacteria. The universal procedure was applied to extract DNA from bacteria which was then sequenced with 16S primers. The aims of the study were to identify bacterial microbiota associated with the PCN populations and to infer their phylogenetic relationships based on the maximum likelihood and Bayesian phylogeny of the 16S sequences. In addition, the impact of the most significant climate and edaphic factors on bacterial diversity were evaluated. Regarding the higher taxonomy, our results indicate that the prevalent bacterial classes were Bacilli, Actinobacteria and Alphaproteobacteria. Phylogenetic analyses clustered Brevibacterium frigoritolerans within the family Bacillaceae, confirming its recent reclassification. Long-term climate factors, such as air temperature, insolation hours, humidity and precipitation, as well as the content of soil organic matter, affected the bacterial diversity. The ability of cyst nematodes to persist in soil for a long time qualifies them as a significant natural source to explore the soil bacterial microbiota.",
publisher = "MDPI",
journal = "Plants",
title = "Bacterial microbiota isolated from cysts of Globodera rostochiensis (Nematoda: Heteroderidae).",
number = "9",
pages = "1146",
volume = "9",
doi = "10.3390%2Fplants9091146"
}

Oro, V., Knezevic, M., Dinić, Z.,& Delić, D.. (2020). Bacterial microbiota isolated from cysts of Globodera rostochiensis (Nematoda: Heteroderidae).. in Plants
MDPI., 9(9), 1146.
https://doi.org/10.3390%2Fplants9091146

Oro V, Knezevic M, Dinić Z, Delić D. Bacterial microbiota isolated from cysts of Globodera rostochiensis (Nematoda: Heteroderidae).. in Plants. 2020;9(9):1146.
doi:10.3390%2Fplants9091146 .

Oro, Violeta, Knezevic, Magdalena, Dinić, Zoran, Delić, Dušica, "Bacterial microbiota isolated from cysts of Globodera rostochiensis (Nematoda: Heteroderidae)." in Plants, 9, no. 9 (2020):1146,
https://doi.org/10.3390%2Fplants9091146 . .

TY  - JOUR
AU  - Stanojković-Sebić, Aleksandra
AU  - Pavlović, Snežana
AU  - Starović, Mira
AU  - Pivić, Radmila
AU  - Dinić, Zoran
AU  - Ljepšanović, Zorica
AU  - Jošić, Dragana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1022
AB  - Fusarium species are widely distributed and responsible for several plant diseases in different medicinal plants. Fungi of this genera cause very important economic losses in Serbian plantation. Antibiotic production by plant-associated microorganisms represents an environmentally acceptable method of disease control, esspecialy in cultivation of medicinal and aromatic plants. Among the plant growth promoting bacteria (PGPB), Pseudomonas have been recognized as the most frequent antagonists of plant fungal pathogens and antibiotic producers. This is probably due to the widely distribution of this diverse group of bacteria in temperate soils and their often predomination among bacteria from plant rhizosphere. In this study, we examined the antifungal activity of eleven indigenous Pseudomonas isolates (PB4, PB5, K38, Q34, PBA12, PD5, C7, C8, Q16P, K29 and K35) against eight phytopathogenic fungi belonging to genus Fusarium (Fusarium tricinctum, F. sambucinum, F. equiseti, F. heterosporum, F. sporotrichioides, F. semitectum, F. verticillioides and F. oxysporum), which had infected anise (Pimpinella anisum L., fam. Apiaceae), using in vitro growth inhibition tests. The obtained results demonstrated that all Pseudomanas isolates showed more or less pronounced antifungal activity, whereby the most pronounced activity was observed for K29 and K35 strains. F. oxysporum and F. verticillioides showed the highest sensitivity to antibiotic-producing Pseudomanas isolates. In general, it has been concluded that studied Pseudomonas isolates have potential in controlling plant diseases caused by Fusarium spp., whereby the bacterial isolates with the highest inhibitory potential will be selected for further experiments.
PB  - University of agronomic Sciences and veterinary medicine of  Bucharest Faculty of Horticulture
T2  - Scientific Papers: Series B. Horticulture
T1  - Antagonistic activity of indigenous Pseudomonas isolates against fusarium species isolated from anise
EP  - 416
SP  - 413
VL  - 41
ER  - 

@article{
author = "Stanojković-Sebić, Aleksandra and Pavlović, Snežana and Starović, Mira and Pivić, Radmila and Dinić, Zoran and Ljepšanović, Zorica and Jošić, Dragana",
year = "2017",
abstract = "Fusarium species are widely distributed and responsible for several plant diseases in different medicinal plants. Fungi of this genera cause very important economic losses in Serbian plantation. Antibiotic production by plant-associated microorganisms represents an environmentally acceptable method of disease control, esspecialy in cultivation of medicinal and aromatic plants. Among the plant growth promoting bacteria (PGPB), Pseudomonas have been recognized as the most frequent antagonists of plant fungal pathogens and antibiotic producers. This is probably due to the widely distribution of this diverse group of bacteria in temperate soils and their often predomination among bacteria from plant rhizosphere. In this study, we examined the antifungal activity of eleven indigenous Pseudomonas isolates (PB4, PB5, K38, Q34, PBA12, PD5, C7, C8, Q16P, K29 and K35) against eight phytopathogenic fungi belonging to genus Fusarium (Fusarium tricinctum, F. sambucinum, F. equiseti, F. heterosporum, F. sporotrichioides, F. semitectum, F. verticillioides and F. oxysporum), which had infected anise (Pimpinella anisum L., fam. Apiaceae), using in vitro growth inhibition tests. The obtained results demonstrated that all Pseudomanas isolates showed more or less pronounced antifungal activity, whereby the most pronounced activity was observed for K29 and K35 strains. F. oxysporum and F. verticillioides showed the highest sensitivity to antibiotic-producing Pseudomanas isolates. In general, it has been concluded that studied Pseudomonas isolates have potential in controlling plant diseases caused by Fusarium spp., whereby the bacterial isolates with the highest inhibitory potential will be selected for further experiments.",
publisher = "University of agronomic Sciences and veterinary medicine of  Bucharest Faculty of Horticulture",
journal = "Scientific Papers: Series B. Horticulture",
title = "Antagonistic activity of indigenous Pseudomonas isolates against fusarium species isolated from anise",
pages = "416-413",
volume = "41"
}

Stanojković-Sebić, A., Pavlović, S., Starović, M., Pivić, R., Dinić, Z., Ljepšanović, Z.,& Jošić, D.. (2017). Antagonistic activity of indigenous Pseudomonas isolates against fusarium species isolated from anise. in Scientific Papers: Series B. Horticulture
University of agronomic Sciences and veterinary medicine of  Bucharest Faculty of Horticulture., 41, 413-416.

Stanojković-Sebić A, Pavlović S, Starović M, Pivić R, Dinić Z, Ljepšanović Z, Jošić D. Antagonistic activity of indigenous Pseudomonas isolates against fusarium species isolated from anise. in Scientific Papers: Series B. Horticulture. 2017;41:413-416..

Stanojković-Sebić, Aleksandra, Pavlović, Snežana, Starović, Mira, Pivić, Radmila, Dinić, Zoran, Ljepšanović, Zorica, Jošić, Dragana, "Antagonistic activity of indigenous Pseudomonas isolates against fusarium species isolated from anise" in Scientific Papers: Series B. Horticulture, 41 (2017):413-416.