TY  - JOUR
AU  - Stikić, Radmila
AU  - Milincić, Danijel
AU  - Kostić, Aleksandar
AU  - Jovanović, Zorica
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
AU  - Djordjević, Nataša
AU  - Savić, Sladjana
AU  - Czekus, Borisz
AU  - Pešić, Mirjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/853
AB  - BACKGROUND: and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.
PB  - Wiley
T2  - Cereal Chemistry
T1  - Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars
EP  - 633
IS  - 3
SP  - 626
VL  - 97
DO  - 10.1002/cche.10278
ER  - 

@article{
author = "Stikić, Radmila and Milincić, Danijel and Kostić, Aleksandar and Jovanović, Zorica and Gašić, Uroš and Tešić, Živoslav and Djordjević, Nataša and Savić, Sladjana and Czekus, Borisz and Pešić, Mirjana",
year = "2020",
abstract = "BACKGROUND: and objectives Quinoa is considered to be a "natural functional food" due to a lot of bioactive compounds that are beneficial for human health by helping prevent the risks of different diseases. The aim of this study was to test the health-promoting characteristics of Puno and Titicaca quinoa seeds grown in Serbia by assessing their phenolic and flavonoid content, antioxidant activities, and potential anticancer effect on human colorectal cancer cells. Findings Thirteen phenolic compounds were identified and quantified in the seeds of both cultivars, but their profile and concentration of individual phenolic compounds differed. Cytotoxic activities were present in both cultivars, whereas the pronounced concentration and time-dependent effects were more expressed in Puno extracts. Conclusions The extracts of the seeds of both cultivars are a rich source of phenolic and flavonoid compounds and with high antioxidant activities. Potent anticancer activity against the human colorectal cancer was expressed in both investigated cultivars. Significance and novelty The study confirmed the health benefit potential of Puno and Titicaca quinoa cultivars, grown in Serbia. These results are the first to demonstrate a potent anticancer activity of quinoa seed extract against the human colorectal cancer cell line HCT-116, as well as the presence of 7 new phenolic and flavonoid compounds.",
publisher = "Wiley",
journal = "Cereal Chemistry",
title = "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars",
pages = "633-626",
number = "3",
volume = "97",
doi = "10.1002/cche.10278"
}

Stikić, R., Milincić, D., Kostić, A., Jovanović, Z., Gašić, U., Tešić, Ž., Djordjević, N., Savić, S., Czekus, B.,& Pešić, M.. (2020). Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry
Wiley., 97(3), 626-633.
https://doi.org/10.1002/cche.10278

Stikić R, Milincić D, Kostić A, Jovanović Z, Gašić U, Tešić Ž, Djordjević N, Savić S, Czekus B, Pešić M. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. in Cereal Chemistry. 2020;97(3):626-633.
doi:10.1002/cche.10278 .

Stikić, Radmila, Milincić, Danijel, Kostić, Aleksandar, Jovanović, Zorica, Gašić, Uroš, Tešić, Živoslav, Djordjević, Nataša, Savić, Sladjana, Czekus, Borisz, Pešić, Mirjana, "Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars" in Cereal Chemistry, 97, no. 3 (2020):626-633,
https://doi.org/10.1002/cche.10278 . .

TY  - JOUR
AU  - Czekus, Borisz
AU  - Pećinar, Ilinka
AU  - Petrović, Ivana
AU  - Paunović, Novica
AU  - Savić, Slađana
AU  - Jovanović, Zorica
AU  - Stikić, Radmila
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/946
AB  - The aim of this study was to investigate the quinoa fruit and seed microstructure, as well as to determine the qualitative composition of quinoa whole seed spatial localisation of food reserves in cultivars Puno and Titicaca using two complementary spectroscopic techniques (Fourier Transform infrared and Raman). The analyses of the seeds also included measurements of the crude proteins and starch contents. The experiment was carried out during the 2016 growing season in rain-fed conditions in the north of Serbia. The analysis of the scores of the principal components based on the Raman spectra revealed two groups in both seed parts (cotyledons and perisperm). The analysis of the loadings highlighted the spectrum region that contributed to the differentiation, e.g. the band at 472 cm(-1) was related to the amylopectin content in the perisperm region. As for the cotyledons, the spectral range from 1100 to 1650 cm(-1) was responsible for genotype differences and it included both the most important bands derived from Amide I, II and quinoa protein with globoid crystals composed of phytin. IR analysis, similar to the analyses of the crude proteins and starch contents in the seeds, failed to reveal any differences in biochemical composition between two analyzed genotypes.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Journal of Cereal Science
T1  - Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization
EP  - 30
SP  - 25
VL  - 87
DO  - 10.1016/j.jcs.2019.02.011
DO  - 0733-5210
ER  - 

@article{
author = "Czekus, Borisz and Pećinar, Ilinka and Petrović, Ivana and Paunović, Novica and Savić, Slađana and Jovanović, Zorica and Stikić, Radmila",
year = "2019",
abstract = "The aim of this study was to investigate the quinoa fruit and seed microstructure, as well as to determine the qualitative composition of quinoa whole seed spatial localisation of food reserves in cultivars Puno and Titicaca using two complementary spectroscopic techniques (Fourier Transform infrared and Raman). The analyses of the seeds also included measurements of the crude proteins and starch contents. The experiment was carried out during the 2016 growing season in rain-fed conditions in the north of Serbia. The analysis of the scores of the principal components based on the Raman spectra revealed two groups in both seed parts (cotyledons and perisperm). The analysis of the loadings highlighted the spectrum region that contributed to the differentiation, e.g. the band at 472 cm(-1) was related to the amylopectin content in the perisperm region. As for the cotyledons, the spectral range from 1100 to 1650 cm(-1) was responsible for genotype differences and it included both the most important bands derived from Amide I, II and quinoa protein with globoid crystals composed of phytin. IR analysis, similar to the analyses of the crude proteins and starch contents in the seeds, failed to reveal any differences in biochemical composition between two analyzed genotypes.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Journal of Cereal Science",
title = "Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization",
pages = "30-25",
volume = "87",
doi = "10.1016/j.jcs.2019.02.011, 0733-5210"
}

Czekus, B., Pećinar, I., Petrović, I., Paunović, N., Savić, S., Jovanović, Z.,& Stikić, R.. (2019). Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization. in Journal of Cereal Science
Academic Press Ltd- Elsevier Science Ltd, London., 87, 25-30.
https://doi.org/10.1016/j.jcs.2019.02.011

Czekus B, Pećinar I, Petrović I, Paunović N, Savić S, Jovanović Z, Stikić R. Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization. in Journal of Cereal Science. 2019;87:25-30.
doi:10.1016/j.jcs.2019.02.011 .

Czekus, Borisz, Pećinar, Ilinka, Petrović, Ivana, Paunović, Novica, Savić, Slađana, Jovanović, Zorica, Stikić, Radmila, "Raman and Fourier transform infrared spectroscopy application to the Puno and Titicaca cvs. of quinoa seed microstructure and perisperm characterization" in Journal of Cereal Science, 87 (2019):25-30,
https://doi.org/10.1016/j.jcs.2019.02.011 . .