TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanovic Jeromela, Ana
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/741
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014–2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains of Xanthomonas spp. to determine nucleotide changes caused by host–pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on other Brassica oleracea hosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape
PB  - Wiley
T2  - Plant Pathology
T1  - New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanovic Jeromela, Ana and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014–2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains of Xanthomonas spp. to determine nucleotide changes caused by host–pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on other Brassica oleracea hosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape",
publisher = "Wiley",
journal = "Plant Pathology",
title = "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanovic Jeromela, A.,& Popović Milovanović, T.. (2021). New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanovic Jeromela A, Popović Milovanović T. New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanovic Jeromela, Ana, Popović Milovanović, Tatjana, "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanovic Jeromela, Ana
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/942
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1458
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanovic Jeromela, Ana and Nikolić, Ivan and Stanković, Slaviša",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1458-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović Milovanović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanovic Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley., 68(8), 1448-1458.
https://doi.org/10.1111/ppa.13064

Popović Milovanović T, Mitrović P, Jelušić A, Dimkić I, Marjanovic Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1458.
doi:10.1111/ppa.13064 .

Popović Milovanović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanovic Jeromela, Ana, Nikolić, Ivan, Stanković, Slaviša, "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1458,
https://doi.org/10.1111/ppa.13064 . .