TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Berenji, J.
AU  - Krstić, Branka
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/898
AB  - In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005–2007 and the most frequent were those of PVY. A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.
PB  - Aepress Sro, Bratislava
T2  - Acta virologica
T1  - Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates
EP  - 347
SP  - 337
VL  - 55
DO  - 10.4149/av_2011_04_337
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Berenji, J. and Krstić, Branka",
year = "2011",
abstract = "In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005–2007 and the most frequent were those of PVY. A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.",
publisher = "Aepress Sro, Bratislava",
journal = "Acta virologica",
title = "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates",
pages = "347-337",
volume = "55",
doi = "10.4149/av_2011_04_337"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Berenji, J.,& Krstić, B.. (2011). Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta virologica
Aepress Sro, Bratislava., 55, 337-347.
https://doi.org/10.4149/av_2011_04_337

Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Berenji J, Krstić B. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta virologica. 2011;55:337-347.
doi:10.4149/av_2011_04_337 .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Berenji, J., Krstić, Branka, "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates" in Acta virologica, 55 (2011):337-347,
https://doi.org/10.4149/av_2011_04_337 . .