TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Pfaf-Dolovac, Erika
AU  - Aleksić, Goran
AU  - Vučurović, Ana
AU  - Starović, Mira
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/560
AB  - It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
PB  - Springer, Dordrecht
T2  - Potato Research
T1  - The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops
EP  - 46
IS  - 1
SP  - 31
VL  - 62
DO  - 10.1007/s11540-018-9395-y
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Pfaf-Dolovac, Erika and Aleksić, Goran and Vučurović, Ana and Starović, Mira",
year = "2019",
abstract = "It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.",
publisher = "Springer, Dordrecht",
journal = "Potato Research",
title = "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops",
pages = "46-31",
number = "1",
volume = "62",
doi = "10.1007/s11540-018-9395-y"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Pfaf-Dolovac, E., Aleksić, G., Vučurović, A.,& Starović, M.. (2019). The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research
Springer, Dordrecht., 62(1), 31-46.
https://doi.org/10.1007/s11540-018-9395-y

Ristić D, Vučurović I, Kuzmanović S, Pfaf-Dolovac E, Aleksić G, Vučurović A, Starović M. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research. 2019;62(1):31-46.
doi:10.1007/s11540-018-9395-y .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Pfaf-Dolovac, Erika, Aleksić, Goran, Vučurović, Ana, Starović, Mira, "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops" in Potato Research, 62, no. 1 (2019):31-46,
https://doi.org/10.1007/s11540-018-9395-y . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Milosavljević, Anja
AU  - Bosković, J.
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/455
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia
EP  - 221
IS  - 1
SP  - 221
VL  - 100
DO  - 10.1094/PDIS-02-15-0209-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Pfaf-Dolovac, Erika and Milosavljević, Anja and Bosković, J. and Jović, Jelena and Mitrović, Milana and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia",
pages = "221-221",
number = "1",
volume = "100",
doi = "10.1094/PDIS-02-15-0209-PDN"
}

Trkulja, N., Pfaf-Dolovac, E., Milosavljević, A., Bosković, J., Jović, J., Mitrović, M.,& Toševski, I.. (2016). First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 221-221.
https://doi.org/10.1094/PDIS-02-15-0209-PDN

Trkulja N, Pfaf-Dolovac E, Milosavljević A, Bosković J, Jović J, Mitrović M, Toševski I. First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease. 2016;100(1):221-221.
doi:10.1094/PDIS-02-15-0209-PDN .

Trkulja, Nenad, Pfaf-Dolovac, Erika, Milosavljević, Anja, Bosković, J., Jović, Jelena, Mitrović, Milana, Toševski, Ivo, "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia" in Plant Disease, 100, no. 1 (2016):221-221,
https://doi.org/10.1094/PDIS-02-15-0209-PDN . .

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Simić, Aleksandar
AU  - Poštić, Dobrivoj
AU  - Zivanović, Tomislav
AU  - Vučković, Savo
AU  - Pfaf-Dolovac, Erika
AU  - Stanisavljević, Rade
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/371
AB  - The study was conducted to examine variability of green forage yield (GFY) and morphological traits (tiller length, internodes length, internodes-stem diameter, leaf width and length) in nine alfalfa varieties from USA and six varieties from Europe. The trials were carried out in Central Serbia during the second (2006-A1) and third year (2007-A2) of alfalfa exploitation. In unfavourable environmental conditions (A1), varieties from the USA had higher coefficient of variability (CV, %) in all cuttings (I CV= 16.93, II CV= 17.13, III CV=13.00, IV CV=21.29), as well as total yield variability (CV=14.33), compared to varieties from Europe (I CV=7.45, II CV=6.47, III CV= 10.65, IV CV= 19.51, for total yield CV=6.43). In the favourable agro-ecological conditions (A2) genetic potential of varieties was manifested, which caused similar yield variability. Variety origin (USA or Europe) had a low effect on variability of the morphological traits. The highest correlation (P  lt = 0.001) was achieved between yield and tiller length, regardless of variety origin.
PB  - Agricultural Research Communication Centre, Karnal
T2  - Legume Research
T1  - Yield and morpnological traits in alfalfa varieties of different origin
EP  - 441
IS  - 4
SP  - 434
VL  - 38
DO  - 10.5958/0976-0571.2015.00128.9
ER  - 

@article{
author = "Štrbanović, Ratibor and Simić, Aleksandar and Poštić, Dobrivoj and Zivanović, Tomislav and Vučković, Savo and Pfaf-Dolovac, Erika and Stanisavljević, Rade",
year = "2015",
abstract = "The study was conducted to examine variability of green forage yield (GFY) and morphological traits (tiller length, internodes length, internodes-stem diameter, leaf width and length) in nine alfalfa varieties from USA and six varieties from Europe. The trials were carried out in Central Serbia during the second (2006-A1) and third year (2007-A2) of alfalfa exploitation. In unfavourable environmental conditions (A1), varieties from the USA had higher coefficient of variability (CV, %) in all cuttings (I CV= 16.93, II CV= 17.13, III CV=13.00, IV CV=21.29), as well as total yield variability (CV=14.33), compared to varieties from Europe (I CV=7.45, II CV=6.47, III CV= 10.65, IV CV= 19.51, for total yield CV=6.43). In the favourable agro-ecological conditions (A2) genetic potential of varieties was manifested, which caused similar yield variability. Variety origin (USA or Europe) had a low effect on variability of the morphological traits. The highest correlation (P  lt = 0.001) was achieved between yield and tiller length, regardless of variety origin.",
publisher = "Agricultural Research Communication Centre, Karnal",
journal = "Legume Research",
title = "Yield and morpnological traits in alfalfa varieties of different origin",
pages = "441-434",
number = "4",
volume = "38",
doi = "10.5958/0976-0571.2015.00128.9"
}

Štrbanović, R., Simić, A., Poštić, D., Zivanović, T., Vučković, S., Pfaf-Dolovac, E.,& Stanisavljević, R.. (2015). Yield and morpnological traits in alfalfa varieties of different origin. in Legume Research
Agricultural Research Communication Centre, Karnal., 38(4), 434-441.
https://doi.org/10.5958/0976-0571.2015.00128.9

Štrbanović R, Simić A, Poštić D, Zivanović T, Vučković S, Pfaf-Dolovac E, Stanisavljević R. Yield and morpnological traits in alfalfa varieties of different origin. in Legume Research. 2015;38(4):434-441.
doi:10.5958/0976-0571.2015.00128.9 .

Štrbanović, Ratibor, Simić, Aleksandar, Poštić, Dobrivoj, Zivanović, Tomislav, Vučković, Savo, Pfaf-Dolovac, Erika, Stanisavljević, Rade, "Yield and morpnological traits in alfalfa varieties of different origin" in Legume Research, 38, no. 4 (2015):434-441,
https://doi.org/10.5958/0976-0571.2015.00128.9 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/302
AB  - During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide.
AB  - Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia
T1  - Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji
EP  - 116
IS  - 3
SP  - 111
VL  - 65
DO  - 10.5937/zasbilj1403111R
ER  - 

@article{
author = "Ristić, Danijela and Pavlović, Snežana and Trkulja, Nenad and Pfaf-Dolovac, Erika and Dolovac, Nenad and Starović, Mira",
year = "2014",
abstract = "During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide., Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia, Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji",
pages = "116-111",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403111R"
}

Ristić, D., Pavlović, S., Trkulja, N., Pfaf-Dolovac, E., Dolovac, N.,& Starović, M.. (2014). Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 111-116.
https://doi.org/10.5937/zasbilj1403111R

Ristić D, Pavlović S, Trkulja N, Pfaf-Dolovac E, Dolovac N, Starović M. Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja. 2014;65(3):111-116.
doi:10.5937/zasbilj1403111R .

Ristić, Danijela, Pavlović, Snežana, Trkulja, Nenad, Pfaf-Dolovac, Erika, Dolovac, Nenad, Starović, Mira, "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia" in Zaštita bilja, 65, no. 3 (2014):111-116,
https://doi.org/10.5937/zasbilj1403111R . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 

@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}

Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S

Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .

Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/325
AB  - C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
AB  - C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and molecular identification of Cercospora apii on celery in Serbia
T1  - Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
EP  - 84
IS  - 2
SP  - 77
VL  - 65
DO  - 10.5937/zasbilj1402077M
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
abstract = "C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank., C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Cercospora apii on celery in Serbia, Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji",
pages = "84-77",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402077M"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(2), 77-84.
https://doi.org/10.5937/zasbilj1402077M

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja. 2014;65(2):77-84.
doi:10.5937/zasbilj1402077M .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "Morphological and molecular identification of Cercospora apii on celery in Serbia" in Zaštita bilja, 65, no. 2 (2014):77-84,
https://doi.org/10.5937/zasbilj1402077M . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/340
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia
EP  - 1157
IS  - 8
SP  - 1157
VL  - 98
DO  - 10.1094/PDIS-02-14-0135-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia",
pages = "1157-1157",
number = "8",
volume = "98",
doi = "10.1094/PDIS-02-14-0135-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1157-1157.
https://doi.org/10.1094/PDIS-02-14-0135-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease. 2014;98(8):1157-1157.
doi:10.1094/PDIS-02-14-0135-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia" in Plant Disease, 98, no. 8 (2014):1157-1157,
https://doi.org/10.1094/PDIS-02-14-0135-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/341
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.
EP  - 1153
IS  - 8
SP  - 1153
VL  - 98
DO  - 10.1094/PDIS-08-13-0858-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.",
pages = "1153-1153",
number = "8",
volume = "98",
doi = "10.1094/PDIS-08-13-0858-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1153-1153.
https://doi.org/10.1094/PDIS-08-13-0858-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease. 2014;98(8):1153-1153.
doi:10.1094/PDIS-08-13-0858-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia." in Plant Disease, 98, no. 8 (2014):1153-1153,
https://doi.org/10.1094/PDIS-08-13-0858-PDN . .

TY  - CONF
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/335
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management
EP  - 34
IS  - 11
SP  - 34
VL  - 104
ER  - 

@conference{
author = "Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management",
pages = "34-34",
number = "11",
volume = "104"
}

Dolovac, N., Trkulja, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 34-34.

Dolovac N, Trkulja N, Milosavljević A, Pfaf-Dolovac E. Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology. 2014;104(11):34-34..

Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management" in Phytopathology, 104, no. 11 (2014):34-34.

TY  - CONF
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/334
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola
EP  - 120
IS  - 11
SP  - 120
VL  - 104
ER  - 

@conference{
author = "Trkulja, Nenad and Dolovac, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola",
pages = "120-120",
number = "11",
volume = "104"
}

Trkulja, N., Dolovac, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 120-120.

Trkulja N, Dolovac N, Milosavljević A, Pfaf-Dolovac E. Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology. 2014;104(11):120-120..

Trkulja, Nenad, Dolovac, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola" in Phytopathology, 104, no. 11 (2014):120-120.

TY  - JOUR
AU  - Labus Blagojevic, Svetlana
AU  - Gavrilovic, Veljko
AU  - Dolovac, Nenad
AU  - Pfaf Dolovac, Erika
AU  - Trkulja, Nenad
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1140
PB  - Serbian Water Pollution Control Society
T2  - Water Research and Management
T1  - Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia
EP  - 50
IS  - 3
SP  - 37
VL  - 3
ER  - 

@article{
author = "Labus Blagojevic, Svetlana and Gavrilovic, Veljko and Dolovac, Nenad and Pfaf Dolovac, Erika and Trkulja, Nenad",
year = "2013",
publisher = "Serbian Water Pollution Control Society",
journal = "Water Research and Management",
title = "Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia",
pages = "50-37",
number = "3",
volume = "3"
}

Labus Blagojevic, S., Gavrilovic, V., Dolovac, N., Pfaf Dolovac, E.,& Trkulja, N.. (2013). Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia. in Water Research and Management
Serbian Water Pollution Control Society., 3(3), 37-50.

Labus Blagojevic S, Gavrilovic V, Dolovac N, Pfaf Dolovac E, Trkulja N. Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia. in Water Research and Management. 2013;3(3):37-50..

Labus Blagojevic, Svetlana, Gavrilovic, Veljko, Dolovac, Nenad, Pfaf Dolovac, Erika, Trkulja, Nenad, "Environmental fate of most commonly used pesticides in the Morava River Basin, Serbia" in Water Research and Management, 3, no. 3 (2013):37-50.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Ivanović, Žarko
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/272
AB  - A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene
EP  - 902
IS  - 4
SP  - 889
VL  - 135
DO  - 10.1007/s10658-012-0135-x
ER  - 

@article{
author = "Trkulja, Nenad and Ivanović, Žarko and Pfaf-Dolovac, Erika and Dolovac, Nenad and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2013",
abstract = "A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene",
pages = "902-889",
number = "4",
volume = "135",
doi = "10.1007/s10658-012-0135-x"
}

Trkulja, N., Ivanović, Ž., Pfaf-Dolovac, E., Dolovac, N., Mitrović, M., Toševski, I.,& Jović, J.. (2013). Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology
Springer, Dordrecht., 135(4), 889-902.
https://doi.org/10.1007/s10658-012-0135-x

Trkulja N, Ivanović Ž, Pfaf-Dolovac E, Dolovac N, Mitrović M, Toševski I, Jović J. Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology. 2013;135(4):889-902.
doi:10.1007/s10658-012-0135-x .

Trkulja, Nenad, Ivanović, Žarko, Pfaf-Dolovac, Erika, Dolovac, Nenad, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene" in European Journal of Plant Pathology, 135, no. 4 (2013):889-902,
https://doi.org/10.1007/s10658-012-0135-x . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/216
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia
EP  - 912
IS  - 6
SP  - 912
VL  - 96
DO  - 10.1094/PDIS-02-12-0179-PDN
ER  - 

@article{
author = "Stevanović, Miloš and Stanković, Ivana and Vučurović, Ana and Dolovac, Nenad and Pfaf-Dolovac, Erika and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia",
pages = "912-912",
number = "6",
volume = "96",
doi = "10.1094/PDIS-02-12-0179-PDN"
}

Stevanović, M., Stanković, I., Vučurović, A., Dolovac, N., Pfaf-Dolovac, E., Krstić, B.,& Bulajić, A.. (2012). First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(6), 912-912.
https://doi.org/10.1094/PDIS-02-12-0179-PDN

Stevanović M, Stanković I, Vučurović A, Dolovac N, Pfaf-Dolovac E, Krstić B, Bulajić A. First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia. in Plant Disease. 2012;96(6):912-912.
doi:10.1094/PDIS-02-12-0179-PDN .

Stevanović, Miloš, Stanković, Ivana, Vučurović, Ana, Dolovac, Nenad, Pfaf-Dolovac, Erika, Krstić, Branka, Bulajić, Aleksandra, "First Report of Oidium neolycopersici on Greenhouse Tomatoes in Serbia" in Plant Disease, 96, no. 6 (2012):912-912,
https://doi.org/10.1094/PDIS-02-12-0179-PDN . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Živković, Svetlana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/224
AB  - Characteristics of pathogenic Pseudomonas bacterial strains isolated from cherry in Serbia are presented in the article. Two types of symptoms were observed on cherry trees at few localities with intensive production in Serbia (Belgrade, Čačak, Topola, Šabac, Novi Sad). The first symptom is bud necrosis and the second bacterial canker of cherry branch. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains were levan and HR positive, while negative results were recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Based on pathogenicity tests and differential GATT tests, investigated strains were divided in two distinct groups: the first group consisted of strains isolated from necrotic cherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits, syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartrate negative (typical characteristics of P.s. pv. syringae). Contrary, second group strains were isolated from necrotic cherry buds, showed negative results in mentioned pathogenicity tests, gelatin and aesculin tests were negative, while tyrosinase and tartrate were positive (typical characteristics of P.s. pv. morsprunorum). REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. s pv. morsprunorum compared to referent strain. In contrast, isolates obtained from necrotic cherry branches had unique fingerprint profiles but different from all reference strains. According to the obtained results it was concluded that both pathovars of P. syringae (syringae and morsprunorum) cause necrosis of cherry trees in Serbia.
AB  - U radu su prikazane patogene, bakteriološke odlike izolata bakterije Pseudomnas syringae poreklom iz trešnje, kao i njihova molekularna karakterizacija primenom REP-PCR metode. Dva tipa simptoma bolesti zapažena su u savremenim zasadima trešnje na području Beograda, Novog Sada, Topole i Šapca. Prvi tip simptoma se ispoljava u vidu nekroze cvetnih i lisnih pupoljaka trešnje, a drugi u vidu nekroze višegodišnjih grana. Iz nekrotičnog tkiva su izolovane Gram negativne bakterije , koje fluoresciraju na King podlozi B, a glukozu metabolišu isključivo u aerobnim uslovima; bakterija stvara levan i prouzrokuje HR duvana ali ne stvara oksidazu, pektinazu u arginin dehidrolazu (LOPAT +---+) što su odlike karakteristične za bakteriju P. syringae. U pogledu patogenosti izolovanih sojeva, kao i njihovih diferencijalnih biohemijskih testova (GATT) proučavani izolati su svrstani u dve jasno izdiferencirane grupe. Prvu čine sojevi izolovani iz nekrozom zahavćenih grana trešnje, koji prouzrokuju nekrozu inokulisanih plodova trešnje, kruške i limuna, listova jorgovana i mahuna boranije. Ova grupa izolata hidrolizuje želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Pomenute patogene i diferencijalne biohemijske odlike su karakteristične za P.s. pv. syringae. Nasuprot njima, sojevi izolovani iz nekrotičnih pupoljaka trešnje negativno reaguju pri pomenutim testovima patogenosti, ne hidrolizuju želatin i eskulin, ali stvaraju tirozinazu i metabolišu tartarate, što su odlike P.s.pv. morsprunorum. REP-PCR analiza takođe ukazuje da sojevi iz nekrotičnih pupoljaka ispoljavaju identične karakteristike kao i referentni soj P.s. pv. morsprunorum. Primenom ovog metoda je takođe utvrđeno da su sojevi izolovani iz obolelih grana trešnje međusobno identični ali da se razlikuju od referentnih sojeva korišćenih u ovim istraživanjima. Na osnovu dobijenih rezultata, tokom ovih istraživanja zaključeno je da oba patogena varijeteta P. syringae patogena voćaka (pv. syrnigae i pv. morsprunorum) parazitiraju trešnju u našim agroekološkim uslovima.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pseudomonas syringae: Pathogen of sweet cherry in Serbia
T1  - Pseudomonas syringae - patogen trešnje u Srbiji
EP  - 149
IS  - 2
SP  - 141
VL  - 27
DO  - 10.2298/PIF1202141G
ER  - 

@article{
author = "Gavrilović, Veljko and Živković, Svetlana and Dolovac, Nenad and Trkulja, Nenad and Pfaf-Dolovac, Erika and Popović, Tatjana and Ivanović, Žarko",
year = "2012",
abstract = "Characteristics of pathogenic Pseudomonas bacterial strains isolated from cherry in Serbia are presented in the article. Two types of symptoms were observed on cherry trees at few localities with intensive production in Serbia (Belgrade, Čačak, Topola, Šabac, Novi Sad). The first symptom is bud necrosis and the second bacterial canker of cherry branch. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains were levan and HR positive, while negative results were recorded for oxidase, pectinase and arginin dihydrolase tests (LOPAT+- - - +). Based on pathogenicity tests and differential GATT tests, investigated strains were divided in two distinct groups: the first group consisted of strains isolated from necrotic cherry branch which caused necrosis on artificially inoculated cherry, pear and lemon fruits, syringae leaves and bean pods, were gelatin and aesculin positive, and tyrosinase and tartrate negative (typical characteristics of P.s. pv. syringae). Contrary, second group strains were isolated from necrotic cherry buds, showed negative results in mentioned pathogenicity tests, gelatin and aesculin tests were negative, while tyrosinase and tartrate were positive (typical characteristics of P.s. pv. morsprunorum). REP PCR analyses showed that strains isolated from necrotic cherry buds belong to P. s pv. morsprunorum compared to referent strain. In contrast, isolates obtained from necrotic cherry branches had unique fingerprint profiles but different from all reference strains. According to the obtained results it was concluded that both pathovars of P. syringae (syringae and morsprunorum) cause necrosis of cherry trees in Serbia., U radu su prikazane patogene, bakteriološke odlike izolata bakterije Pseudomnas syringae poreklom iz trešnje, kao i njihova molekularna karakterizacija primenom REP-PCR metode. Dva tipa simptoma bolesti zapažena su u savremenim zasadima trešnje na području Beograda, Novog Sada, Topole i Šapca. Prvi tip simptoma se ispoljava u vidu nekroze cvetnih i lisnih pupoljaka trešnje, a drugi u vidu nekroze višegodišnjih grana. Iz nekrotičnog tkiva su izolovane Gram negativne bakterije , koje fluoresciraju na King podlozi B, a glukozu metabolišu isključivo u aerobnim uslovima; bakterija stvara levan i prouzrokuje HR duvana ali ne stvara oksidazu, pektinazu u arginin dehidrolazu (LOPAT +---+) što su odlike karakteristične za bakteriju P. syringae. U pogledu patogenosti izolovanih sojeva, kao i njihovih diferencijalnih biohemijskih testova (GATT) proučavani izolati su svrstani u dve jasno izdiferencirane grupe. Prvu čine sojevi izolovani iz nekrozom zahavćenih grana trešnje, koji prouzrokuju nekrozu inokulisanih plodova trešnje, kruške i limuna, listova jorgovana i mahuna boranije. Ova grupa izolata hidrolizuje želatin i eskulin, ali ne stvaraju tirozinazu i ne metabolišu tartarate. Pomenute patogene i diferencijalne biohemijske odlike su karakteristične za P.s. pv. syringae. Nasuprot njima, sojevi izolovani iz nekrotičnih pupoljaka trešnje negativno reaguju pri pomenutim testovima patogenosti, ne hidrolizuju želatin i eskulin, ali stvaraju tirozinazu i metabolišu tartarate, što su odlike P.s.pv. morsprunorum. REP-PCR analiza takođe ukazuje da sojevi iz nekrotičnih pupoljaka ispoljavaju identične karakteristike kao i referentni soj P.s. pv. morsprunorum. Primenom ovog metoda je takođe utvrđeno da su sojevi izolovani iz obolelih grana trešnje međusobno identični ali da se razlikuju od referentnih sojeva korišćenih u ovim istraživanjima. Na osnovu dobijenih rezultata, tokom ovih istraživanja zaključeno je da oba patogena varijeteta P. syringae patogena voćaka (pv. syrnigae i pv. morsprunorum) parazitiraju trešnju u našim agroekološkim uslovima.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pseudomonas syringae: Pathogen of sweet cherry in Serbia, Pseudomonas syringae - patogen trešnje u Srbiji",
pages = "149-141",
number = "2",
volume = "27",
doi = "10.2298/PIF1202141G"
}

Gavrilović, V., Živković, S., Dolovac, N., Trkulja, N., Pfaf-Dolovac, E., Popović, T.,& Ivanović, Ž.. (2012). Pseudomonas syringae: Pathogen of sweet cherry in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 27(2), 141-149.
https://doi.org/10.2298/PIF1202141G

Gavrilović V, Živković S, Dolovac N, Trkulja N, Pfaf-Dolovac E, Popović T, Ivanović Ž. Pseudomonas syringae: Pathogen of sweet cherry in Serbia. in Pesticidi i fitomedicina. 2012;27(2):141-149.
doi:10.2298/PIF1202141G .

Gavrilović, Veljko, Živković, Svetlana, Dolovac, Nenad, Trkulja, Nenad, Pfaf-Dolovac, Erika, Popović, Tatjana, Ivanović, Žarko, "Pseudomonas syringae: Pathogen of sweet cherry in Serbia" in Pesticidi i fitomedicina, 27, no. 2 (2012):141-149,
https://doi.org/10.2298/PIF1202141G . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Oro, Violeta
AU  - Pfaf-Dolovac, Erika
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/246
AB  - Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum.
AB  - Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Identification of Colletotrichum acutatum from nectarine fruit
T1  - Identifikacija Colletotrichum acutatum sa ploda nektarine
EP  - 138
IS  - 3
SP  - 130
VL  - 63
ER  - 

@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Oro, Violeta and Pfaf-Dolovac, Erika and Stošić, Stefan and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum., Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Identification of Colletotrichum acutatum from nectarine fruit, Identifikacija Colletotrichum acutatum sa ploda nektarine",
pages = "138-130",
number = "3",
volume = "63"
}

Živković, S., Gavrilović, V., Oro, V., Pfaf-Dolovac, E., Stošić, S., Kuzmanović, S.,& Ivanović, Ž.. (2012). Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 130-138.

Živković S, Gavrilović V, Oro V, Pfaf-Dolovac E, Stošić S, Kuzmanović S, Ivanović Ž. Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja. 2012;63(3):130-138..

Živković, Svetlana, Gavrilović, Veljko, Oro, Violeta, Pfaf-Dolovac, Erika, Stošić, Stefan, Kuzmanović, Slobodan, Ivanović, Žarko, "Identification of Colletotrichum acutatum from nectarine fruit" in Zaštita bilja, 63, no. 3 (2012):130-138.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Pfaf Dolovac, Erika
AU  - Stevanović, Miloš
AU  - Ivanović, Žarko
AU  - Štrbanović, Ratibor
AU  - Zivkovic, Svetlana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1080
AB  - Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 – 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 – 14.29 %.
PB  - Institut za zaštitu bilja i životnu sredinu
T2  - Zaštita bilja
T1  - Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima
EP  - 117
IS  - 2
SP  - 109
VL  - 62
ER  - 

@article{
author = "Trkulja, Nenad and Dolovac, Nenad and Pfaf Dolovac, Erika and Stevanović, Miloš and Ivanović, Žarko and Štrbanović, Ratibor and Zivkovic, Svetlana",
year = "2011",
abstract = "Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 – 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 – 14.29 %.",
publisher = "Institut za zaštitu bilja i životnu sredinu",
journal = "Zaštita bilja",
title = "Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima",
pages = "117-109",
number = "2",
volume = "62"
}

Trkulja, N., Dolovac, N., Pfaf Dolovac, E., Stevanović, M., Ivanović, Ž., Štrbanović, R.,& Zivkovic, S.. (2011). Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu., 62(2), 109-117.

Trkulja N, Dolovac N, Pfaf Dolovac E, Stevanović M, Ivanović Ž, Štrbanović R, Zivkovic S. Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima. in Zaštita bilja. 2011;62(2):109-117..

Trkulja, Nenad, Dolovac, Nenad, Pfaf Dolovac, Erika, Stevanović, Miloš, Ivanović, Žarko, Štrbanović, Ratibor, Zivkovic, Svetlana, "Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima" in Zaštita bilja, 62, no. 2 (2011):109-117.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Stevanović, Miloš
AU  - Ivanović, Žarko
AU  - Štrbanović, Ratibor
AU  - Živković, Svetlana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/148
AB  - Cercospora leaf spot is the most important disease that occurs on sugar beet in the world. Damages caused by this disease can reduce yields up to 50 %. Resistance to the group of benzimidazole fungicide was determined in 1976. Last twenty years their use has been reduced but they're still used, mainly formulated in mixture with fungicides - sterol demethylation inhibitors (DMI). Sterol demethylation inhibitors (DMI) have been used extensively in the last two decades to control C. beticola in the world, and resistance to them was observed in 2000. The results showed that populations of C. beticola resistant to benzimidazole are present in sugar beet fields and are the dominant population, with the prevalence of resistance 83.87 - 94.28 %. The frequency of resistance to DMI fungicides is smaller, but highly significant and amounts to 12.90 - 14.29 %.
AB  - Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 - 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 - 14.29 %.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides
T1  - Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima
EP  - 117
IS  - 2
SP  - 109
VL  - 62
ER  - 

@article{
author = "Trkulja, Nenad and Dolovac, Nenad and Pfaf-Dolovac, Erika and Stevanović, Miloš and Ivanović, Žarko and Štrbanović, Ratibor and Živković, Svetlana",
year = "2011",
abstract = "Cercospora leaf spot is the most important disease that occurs on sugar beet in the world. Damages caused by this disease can reduce yields up to 50 %. Resistance to the group of benzimidazole fungicide was determined in 1976. Last twenty years their use has been reduced but they're still used, mainly formulated in mixture with fungicides - sterol demethylation inhibitors (DMI). Sterol demethylation inhibitors (DMI) have been used extensively in the last two decades to control C. beticola in the world, and resistance to them was observed in 2000. The results showed that populations of C. beticola resistant to benzimidazole are present in sugar beet fields and are the dominant population, with the prevalence of resistance 83.87 - 94.28 %. The frequency of resistance to DMI fungicides is smaller, but highly significant and amounts to 12.90 - 14.29 %., Pegavost lišća šećerne repe je najznačajnija bolest koja se javlja na šećernoj repi u svetu. Štete koje nastaju usled pojave bolesti mogu smanjiti prinos i do 50%. Prema fungicidima iz grupe benzimidazola rezistentnost je utvrđena 1976. godine. Poslednjih dvadeset godina njihova upotreba je redukovana, ali se i dalje koriste uglavnom formulisani u mešavini sa fungicidima inhibitorima sinteze sterola (DMI). Fungicidi inhibitori demetilacije sterola (DMI), se zadnje dve decenije intenzivno koriste za suzbijanje C. beticola u svetu, a pojava rezistentnosti prema njima konstatovana je 2000. godine. Rezultati istraživanja su pokazali da su populacije C. beticola rezistentne prema benzimidazolima prisutne na poljima šećerne repe i čine dominantnu populaciju uz učestalost rezistentnosti 83.87 - 94.28 %. Učestalost rezistentnosti prema DMI fungicidima je manja, ali veoma značajna i iznosi 12.90 - 14.29 %.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides, Učestalost rezistentnosti Cercospora beticola (Sacc.) prema benzimidazolima i DMI fungicidima",
pages = "117-109",
number = "2",
volume = "62"
}

Trkulja, N., Dolovac, N., Pfaf-Dolovac, E., Stevanović, M., Ivanović, Ž., Štrbanović, R.,& Živković, S.. (2011). Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(2), 109-117.

Trkulja N, Dolovac N, Pfaf-Dolovac E, Stevanović M, Ivanović Ž, Štrbanović R, Živković S. Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides. in Zaštita bilja. 2011;62(2):109-117..

Trkulja, Nenad, Dolovac, Nenad, Pfaf-Dolovac, Erika, Stevanović, Miloš, Ivanović, Žarko, Štrbanović, Ratibor, Živković, Svetlana, "Frequency of resistance Cercospora beticola (Sacc.) populations to benzimidazole and DMI fungicides" in Zaštita bilja, 62, no. 2 (2011):109-117.

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Miletić, Novica
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/192
AB  - Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia.
AB  - Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia
T1  - Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji
EP  - 346
IS  - 4
SP  - 337
VL  - 26
DO  - 10.2298/PIF1104337D
ER  - 

@article{
author = "Dolovac, Nenad and Miletić, Novica and Trkulja, Nenad and Pfaf-Dolovac, Erika and Živković, Svetlana and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "Peach rusty spot has regularly occurred in Serbia in recent years, causing significant economic losses in the production of late maturing cultivars (Summerset, Suncrest, Fayette and O'Henry). Concerns regarding the etiology of this disease have been solved using molecular identification and cross-pathogenicity tests, which have proved that peach rusty spot is caused by Podosphaera leucotricha. The usual recommended strategy for its control, by multiple treatments starting from petal fall phenophase, has not proved satisfactory in agro-ecological conditions in Serbia. After testing the fungicide efficacy, a three-year study was carried out to determine the precise treatment terms and make the control of P. leucotricha more efficient. The investigation was conducted in the conditions of natural infection in a Summerset cultivar orchard at the locality of Bela Crkva, during the period 2006-2008. Nine different variants of both single and mixed treatments were tested, covering six defined peach growth phenophases - from green top, until the phenophase of second fruit falling. In the conditions of high levels of natural infection in the control plots, in all three investigation years the most efficient was the variant in which kresoxim-methyl was applied three times: in green top, pink bud and full bloom phenophases (91.69 - 91.92%), followed by the variant of treatments in the phenophases of rose bud, full bloom and petal fall (86.3-87.87%) and the variant of two treatments, performed in the phenophases of pink bud and full bloom (79.3-83.09%). The other tested variants showed significantly lower efficiency. The results obtained could significantly contribute to better peach fruit production, without losses caused by peach rusty spot which is commercially very important disease in Serbia., Rđasta mrežavost plodova breskve poslednjih godina u Srbiji redovno se javlja i u proizvodnji poznih sorti (Summerset, Suncrest, Fayette i O'Henry) nanoseći značajne ekonomske gubitke. Nedoumice u pogledu etiologije ove bolesti razrešene su na osnovu molekularne identifikacije i unakrsnih testova patogenosti čime je dokazano da rđastu mrežavost breskve izaziva Podosphaera leucotricha. Strategija kontrole P. leucotricha primenjena u svetu, po kojoj suzbijanje treba sprovoditi u višekratnim tretmanima od fenofaze precvetavanja, nije dala zadovoljavajuće rezultate u agroekološkim uslovima Srbije. Nakon izvršenih ispitivanja efikasnosti fungicida, obavljeno je trogodišnje istraživanje u cilju određivanja preciznih rokova tretiranja breskve radi uspostavljanja efikasne kontrole P. leucotricha. Ispitivanje je obavljeno u periodu od 2006. do 2008. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je devet različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od šest definisanih fenofaza razvoja breskve, počevši od fenofaze zeleni vrh do fenofaze drugo opadanje plodova. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost u sve tri godine ispitivanja ispoljila je varijanta u kojoj je kresoksim- metil primenjivan tri puta, u fenofazi zeleni vrh, roze pupoljak i cvetanje (91,69-91,92%), odmah zatim varijanta u kojoj su izvođeni tretmani u fenofazi roze pupoljak, cvetanje i precvetavanje (86,3-87,87%) i varijanta u okviru koje su bila vršena dva tretmana u fenofazi roze pupoljak i cvetanje (79,3-83,09%). Ostale varijante u ogledu ispoljile su znatno nižu efikasnost. Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve bez gubitaka izazvanih rđastom mrežavosti ploda, ekonomski sve značajnijeg oboljenja u Srbiji.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia, Optimalni rokovi primene fungicida za suzbijanje Podosphaera leucotricha, prouzrokovača rđaste mrežavosti plodova breskve u Srbiji",
pages = "346-337",
number = "4",
volume = "26",
doi = "10.2298/PIF1104337D"
}

Dolovac, N., Miletić, N., Trkulja, N., Pfaf-Dolovac, E., Živković, S., Krstić, B.,& Bulajić, A.. (2011). Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 26(4), 337-346.
https://doi.org/10.2298/PIF1104337D

Dolovac N, Miletić N, Trkulja N, Pfaf-Dolovac E, Živković S, Krstić B, Bulajić A. Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia. in Pesticidi i fitomedicina. 2011;26(4):337-346.
doi:10.2298/PIF1104337D .

Dolovac, Nenad, Miletić, Novica, Trkulja, Nenad, Pfaf-Dolovac, Erika, Živković, Svetlana, Krstić, Branka, Bulajić, Aleksandra, "Optimal timing of fungicide applications for the control of Podosphaera leucotricha, the causal agent of peach rusty spot in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):337-346,
https://doi.org/10.2298/PIF1104337D . .

TY  - JOUR
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Aleksić, Goran
AU  - Stevanović, Miloš
AU  - Pfaf-Dolovac, Erika
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/185
AB  - The causal agent of peach leaf curl Taphrina deformans, in recent years in Serbia occurs regularly and causes significant economic losses. As the leaf curl important in many areas of growing peaches in the world, great attention is focused on finding a measure of control. According to recommendations from the literature sources of chemical protection against this pathogen should be carried out in several treatments, ie. in the fall after the leaves fall and the spring just before and during the swelling buds. As is well known that the pathogen T. deformans infection achieved solely through the undifferentiated tissue swelling in the bud stage of development, there were determined the effectiveness of fungicides recommended in all phenological stages of development. The study was conducted during fall 2006. and spring of 2007. year, the Summerset peach orchards cultivars in conditions of natural infection at the site of Bela Crkva. We tested seven different varieties, which are comprised of individual and combined treatments, so that the period covered by the three defined phenological stages of peach, starting from autumn treatment stage of development in 50% of leaves yellow and dorman and growth stages of vegetation where the applied fungicide copper hidroksid, and phenophase bud swell to when fungicide was applied ziram. In conditions of high levels of natural infection in the control plots, the highest efficiency demonstrated the variant that contained the treatment of swelling in the bud stage of development (96.22 - 97.84%), while other variants did not show satisfactory efficiency (7.78 - 16.85% ). These results significantly contribute to an improved method of groweing peach with substantial savings on chemicals without losses caused by disease, peach leaf curl.
AB  - Prouzrokovač kovrdžavosti lista breskve Taphrina deformans, poslednjih godina u Srbiji redovno se javlja i nanosi značajne ekonomske gubitke. Kako je kovrdžavost lista značajna u mnogim područjima gajenja breskve u svetu velika pažnja usmerena je na iznalaženje mera kontrole. Prema preporukama iz literaturnih izvora hemijsku zaštitu protiv ovog patogena treba sprovoditi u nekoliko tretmana, tj. u jesen nakon opadanja lišća i u proleće neposredno pre i u toku bubrenja pupoljaka. Kako je poznato da patogen T. deformans ostvaruje infekciju isključivo preko nediferenciranog tkiva u fenofazi bubrenja pupoljka, vršena su ispitivanja efikasnosti primene fungicida u svim preporučenim fenofazama razvoja. Ispitivanje je obavljeno u periodu jesen 2006. i proleće 2007. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je sedam različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od tri definisane fenofaze razvoja breskve, počevši od jesenjeg tretmana u fenofazi 50% lišća žuto i opalo i fenofaze mirovanja vegetacije gde je primenjen fungicid bakar hidroksid do fenofaze bubrenje pupoljka kada je primenjen fungicid ciram. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost ispoljile su varijante koje su sadržale tretman u fenofazi bubrenja pupoljka (96,22 - 97,84%), dok ostale varijante nisu pokazale zadovoljavajuću efikasnost (7,78 - 16,85%). Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve uz značajnu uštedu hemijskih sredstava bez gubitaka izazvanih oboljenjem kovrdžavosti lista breskve.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia
T1  - Efikasnost rokova primene fungicida za suzbijanje Taphrina deformans, prouzrokovača kovrdžavosti lista breskve u Srbiji
EP  - 226
IS  - 4
SP  - 219
VL  - 62
ER  - 

@article{
author = "Dolovac, Nenad and Trkulja, Nenad and Aleksić, Goran and Stevanović, Miloš and Pfaf-Dolovac, Erika and Popović, Tatjana and Ivanović, Žarko",
year = "2011",
abstract = "The causal agent of peach leaf curl Taphrina deformans, in recent years in Serbia occurs regularly and causes significant economic losses. As the leaf curl important in many areas of growing peaches in the world, great attention is focused on finding a measure of control. According to recommendations from the literature sources of chemical protection against this pathogen should be carried out in several treatments, ie. in the fall after the leaves fall and the spring just before and during the swelling buds. As is well known that the pathogen T. deformans infection achieved solely through the undifferentiated tissue swelling in the bud stage of development, there were determined the effectiveness of fungicides recommended in all phenological stages of development. The study was conducted during fall 2006. and spring of 2007. year, the Summerset peach orchards cultivars in conditions of natural infection at the site of Bela Crkva. We tested seven different varieties, which are comprised of individual and combined treatments, so that the period covered by the three defined phenological stages of peach, starting from autumn treatment stage of development in 50% of leaves yellow and dorman and growth stages of vegetation where the applied fungicide copper hidroksid, and phenophase bud swell to when fungicide was applied ziram. In conditions of high levels of natural infection in the control plots, the highest efficiency demonstrated the variant that contained the treatment of swelling in the bud stage of development (96.22 - 97.84%), while other variants did not show satisfactory efficiency (7.78 - 16.85% ). These results significantly contribute to an improved method of groweing peach with substantial savings on chemicals without losses caused by disease, peach leaf curl., Prouzrokovač kovrdžavosti lista breskve Taphrina deformans, poslednjih godina u Srbiji redovno se javlja i nanosi značajne ekonomske gubitke. Kako je kovrdžavost lista značajna u mnogim područjima gajenja breskve u svetu velika pažnja usmerena je na iznalaženje mera kontrole. Prema preporukama iz literaturnih izvora hemijsku zaštitu protiv ovog patogena treba sprovoditi u nekoliko tretmana, tj. u jesen nakon opadanja lišća i u proleće neposredno pre i u toku bubrenja pupoljaka. Kako je poznato da patogen T. deformans ostvaruje infekciju isključivo preko nediferenciranog tkiva u fenofazi bubrenja pupoljka, vršena su ispitivanja efikasnosti primene fungicida u svim preporučenim fenofazama razvoja. Ispitivanje je obavljeno u periodu jesen 2006. i proleće 2007. godine, u zasadu breskve sorte Summerset, u uslovima prirodne zaraze na lokalitetu Bela Crkva. Testirano je sedam različitih varijanti, koje su se sastojale iz kombinovanih i pojedinačnih tretiranja, tako da je pokriven period od tri definisane fenofaze razvoja breskve, počevši od jesenjeg tretmana u fenofazi 50% lišća žuto i opalo i fenofaze mirovanja vegetacije gde je primenjen fungicid bakar hidroksid do fenofaze bubrenje pupoljka kada je primenjen fungicid ciram. U uslovima visokog nivoa prirodne zaraze u kontrolnim parcelama, najvišu efikasnost ispoljile su varijante koje su sadržale tretman u fenofazi bubrenja pupoljka (96,22 - 97,84%), dok ostale varijante nisu pokazale zadovoljavajuću efikasnost (7,78 - 16,85%). Dobijeni rezultati značajno doprinose poboljšanom načinu proizvodnje breskve uz značajnu uštedu hemijskih sredstava bez gubitaka izazvanih oboljenjem kovrdžavosti lista breskve.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia, Efikasnost rokova primene fungicida za suzbijanje Taphrina deformans, prouzrokovača kovrdžavosti lista breskve u Srbiji",
pages = "226-219",
number = "4",
volume = "62"
}

Dolovac, N., Trkulja, N., Aleksić, G., Stevanović, M., Pfaf-Dolovac, E., Popović, T.,& Ivanović, Ž.. (2011). The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 62(4), 219-226.

Dolovac N, Trkulja N, Aleksić G, Stevanović M, Pfaf-Dolovac E, Popović T, Ivanović Ž. The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia. in Zaštita bilja. 2011;62(4):219-226..

Dolovac, Nenad, Trkulja, Nenad, Aleksić, Goran, Stevanović, Miloš, Pfaf-Dolovac, Erika, Popović, Tatjana, Ivanović, Žarko, "The efficacy of fungicide treatment terms for control Taphrina deformans, causal agent of peach leaf curl disease in Serbia" in Zaštita bilja, 62, no. 4 (2011):219-226.

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Jović, Jelena
AU  - Mitrović, Milana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/149
AB  - During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.
PB  - Alma Mater Studiorum, Univ Bologna, Bologna
T2  - Bulletin of Insectology
T1  - First report of stolbur phytoplasma infecting celery in Serbia
VL  - 64
ER  - 

@article{
author = "Ivanović, Žarko and Trkulja, Nenad and Živković, Svetlana and Pfaf-Dolovac, Erika and Dolovac, Nenad and Jović, Jelena and Mitrović, Milana",
year = "2011",
abstract = "During field survey conducted in 2010 in Serbia, symptoms of foliar reddening were observed on celery on the locality Padinska Skela, in the vicinity of Belgrade. Leaf samples from six symptomatic and two asymptomatic plants were collected and tested for phytoplasma presence detection. Nested polymerase chain reaction analyzes using universal primer pairs P1P7 followed by R16F2n/R2 identified presence of phytoplasmas in all symptomatic plants, while asymptomatic plants were tested negative. Restriction analysis of amplified 16Sr DNA fragments with enzyme Msel identified in all positive samples the same pattern as a reference strain of stolbur phytoplasma belonging to the 16SrXII-A ribosomal subgroup. Molecular differentiation of stolbur phytoplasma detected in celery was performed by amplification and RFLP analysis of the elongation factor Tu (tuf gene). Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic celery plants. This is the first record of stolbur phytoplasma in association with celery expressing symptoms of foliar reddening in Serbia.",
publisher = "Alma Mater Studiorum, Univ Bologna, Bologna",
journal = "Bulletin of Insectology",
title = "First report of stolbur phytoplasma infecting celery in Serbia",
volume = "64"
}

Ivanović, Ž., Trkulja, N., Živković, S., Pfaf-Dolovac, E., Dolovac, N., Jović, J.,& Mitrović, M.. (2011). First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology
Alma Mater Studiorum, Univ Bologna, Bologna., 64.

Ivanović Ž, Trkulja N, Živković S, Pfaf-Dolovac E, Dolovac N, Jović J, Mitrović M. First report of stolbur phytoplasma infecting celery in Serbia. in Bulletin of Insectology. 2011;64..

Ivanović, Žarko, Trkulja, Nenad, Živković, Svetlana, Pfaf-Dolovac, Erika, Dolovac, Nenad, Jović, Jelena, Mitrović, Milana, "First report of stolbur phytoplasma infecting celery in Serbia" in Bulletin of Insectology, 64 (2011).

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Ivanović, Žarko
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Živković, Svetlana
AU  - Jović, Jelena
AU  - Mitrović, Milana
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/157
AB  - Kale plants (Brassica oleracea var. gemmifera L.) showing symptoms of reddening on leaf and petioles and overall stunting were found on locality Pancevo (south Banat) during inspection on vegetable crops carried out in 2010 in Serbia. The phytoplasma associated with the disease was detected through nested PCR with primer pairs P1/P7 and R16F2n/R2 followed by RFLP analysis with restriction enzyme Msel. A total of 10 symptomatic and 4 asymptomatic plants were collected and analyzed for the presence of phytoplasma. Nested PCR amplified 16S ribosomal RNA fragments of phytoplasma in all samples of kale with symptoms, while all asymptomatic plants tested negative. Digestion with Msel identified in infected kale the same pattern as a reference strain of stolbur phytoplasma belonging to 16SrXII-A group. Amplification of the elongation factor tuf gene and digestion with HpaII restriction enzyme was performed for genotype differentiation of stolbur phytoplasmas detected in kale. Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic kale plants. This is the first report of stolbur phytoplasma infecting kale crops in Serbia.
PB  - Alma Mater Studiorum, Univ Bologna, Bologna
T2  - Bulletin of Insectology
T1  - Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia
VL  - 64
ER  - 

@article{
author = "Trkulja, Nenad and Ivanović, Žarko and Pfaf-Dolovac, Erika and Dolovac, Nenad and Živković, Svetlana and Jović, Jelena and Mitrović, Milana",
year = "2011",
abstract = "Kale plants (Brassica oleracea var. gemmifera L.) showing symptoms of reddening on leaf and petioles and overall stunting were found on locality Pancevo (south Banat) during inspection on vegetable crops carried out in 2010 in Serbia. The phytoplasma associated with the disease was detected through nested PCR with primer pairs P1/P7 and R16F2n/R2 followed by RFLP analysis with restriction enzyme Msel. A total of 10 symptomatic and 4 asymptomatic plants were collected and analyzed for the presence of phytoplasma. Nested PCR amplified 16S ribosomal RNA fragments of phytoplasma in all samples of kale with symptoms, while all asymptomatic plants tested negative. Digestion with Msel identified in infected kale the same pattern as a reference strain of stolbur phytoplasma belonging to 16SrXII-A group. Amplification of the elongation factor tuf gene and digestion with HpaII restriction enzyme was performed for genotype differentiation of stolbur phytoplasmas detected in kale. Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic kale plants. This is the first report of stolbur phytoplasma infecting kale crops in Serbia.",
publisher = "Alma Mater Studiorum, Univ Bologna, Bologna",
journal = "Bulletin of Insectology",
title = "Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia",
volume = "64"
}

Trkulja, N., Ivanović, Ž., Pfaf-Dolovac, E., Dolovac, N., Živković, S., Jović, J.,& Mitrović, M.. (2011). Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia. in Bulletin of Insectology
Alma Mater Studiorum, Univ Bologna, Bologna., 64.

Trkulja N, Ivanović Ž, Pfaf-Dolovac E, Dolovac N, Živković S, Jović J, Mitrović M. Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia. in Bulletin of Insectology. 2011;64..

Trkulja, Nenad, Ivanović, Žarko, Pfaf-Dolovac, Erika, Dolovac, Nenad, Živković, Svetlana, Jović, Jelena, Mitrović, Milana, "Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia" in Bulletin of Insectology, 64 (2011).

TY  - JOUR
AU  - Pavlović, Danijela
AU  - Nikolić, Bogdan
AU  - Pfaf-Dolovac, Erika
AU  - Marisavljević, Dragana
AU  - Milićević, Zoran
AU  - Đurović, Sanja
PY  - 2010
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/130
AB  - Chlorophyll is a green pigment important in photosynthesis. By monitoring chlorophyll amount we learn about productivity of photosynthesis. Many herbicides with different modes of action affect the amount of chlorophyll in plant tissue. In our study we examined the amount of chlorophyll in corn plants after application of glyphosate trimesium (sulfosate). We used a non-destructive method: SPAD reading and chlorophyll a fluorescence, and a destructive method: extraction of chlorophyll with dimethil formamide and methanol. Our results show that based on amount of photosynthesis pigments and chlorophyll fluorescence yield we can monitor the stress effect of herbicides on corn plants. Our results also show that we can differentiate between treated and non treated plants.
AB  - Hlorofil je zeleni pigment važan za proces fotosinteze, pa praćenjem njegovog sadržaja u biljkama dobijamo odgovor o produktivnosti fotosinteze. Herbicidi različitih hemijskih grupa i mehanizama dejstva utiču na sadržaj hlorofila u biljnom tkivu. Ispitivan je sadržaj hlorofila u tkivu biljaka kukuruza nakon primene glifosat trimezijum (sulfosat). Primenjene su nedestruktivne metode: SPAD očitavanje sadržaja i merenje fluorescencije hlorofila a, kao i destruktivne: ekstrakcija hlorofila dimetilformamidom i metanolom. Dobijeni rezultati i izvedena istraživanja pokazuju da se na osnovu sadržaja fotosintetskih pigmenata i prinosa fluorescencije hlorofila može pratiti stresni efekat herbicida na gajene biljke kukuruza, kao i definisati nivo razlika između tretiranih i netretiranih biljaka.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Chlorophyll as indicator of plants response to herbicide
T1  - Hlorofil kao indikator reakcije biljaka na herbicide
EP  - 86
IS  - 2
SP  - 67
VL  - 61
ER  - 

@article{
author = "Pavlović, Danijela and Nikolić, Bogdan and Pfaf-Dolovac, Erika and Marisavljević, Dragana and Milićević, Zoran and Đurović, Sanja",
year = "2010",
abstract = "Chlorophyll is a green pigment important in photosynthesis. By monitoring chlorophyll amount we learn about productivity of photosynthesis. Many herbicides with different modes of action affect the amount of chlorophyll in plant tissue. In our study we examined the amount of chlorophyll in corn plants after application of glyphosate trimesium (sulfosate). We used a non-destructive method: SPAD reading and chlorophyll a fluorescence, and a destructive method: extraction of chlorophyll with dimethil formamide and methanol. Our results show that based on amount of photosynthesis pigments and chlorophyll fluorescence yield we can monitor the stress effect of herbicides on corn plants. Our results also show that we can differentiate between treated and non treated plants., Hlorofil je zeleni pigment važan za proces fotosinteze, pa praćenjem njegovog sadržaja u biljkama dobijamo odgovor o produktivnosti fotosinteze. Herbicidi različitih hemijskih grupa i mehanizama dejstva utiču na sadržaj hlorofila u biljnom tkivu. Ispitivan je sadržaj hlorofila u tkivu biljaka kukuruza nakon primene glifosat trimezijum (sulfosat). Primenjene su nedestruktivne metode: SPAD očitavanje sadržaja i merenje fluorescencije hlorofila a, kao i destruktivne: ekstrakcija hlorofila dimetilformamidom i metanolom. Dobijeni rezultati i izvedena istraživanja pokazuju da se na osnovu sadržaja fotosintetskih pigmenata i prinosa fluorescencije hlorofila može pratiti stresni efekat herbicida na gajene biljke kukuruza, kao i definisati nivo razlika između tretiranih i netretiranih biljaka.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Chlorophyll as indicator of plants response to herbicide, Hlorofil kao indikator reakcije biljaka na herbicide",
pages = "86-67",
number = "2",
volume = "61"
}

Pavlović, D., Nikolić, B., Pfaf-Dolovac, E., Marisavljević, D., Milićević, Z.,& Đurović, S.. (2010). Chlorophyll as indicator of plants response to herbicide. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 61(2), 67-86.

Pavlović D, Nikolić B, Pfaf-Dolovac E, Marisavljević D, Milićević Z, Đurović S. Chlorophyll as indicator of plants response to herbicide. in Zaštita bilja. 2010;61(2):67-86..

Pavlović, Danijela, Nikolić, Bogdan, Pfaf-Dolovac, Erika, Marisavljević, Dragana, Milićević, Zoran, Đurović, Sanja, "Chlorophyll as indicator of plants response to herbicide" in Zaštita bilja, 61, no. 2 (2010):67-86.

TY  - JOUR
AU  - Marisavljević, Dragana
AU  - Stojanović, Slobodanka
AU  - Pavlović, Danijela
AU  - Pfaf-Dolovac, Erika
PY  - 2007
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/52
AB  - Giant sumpweed (Iva xanthifolia Nutt.) is a newly-introduced invasive weed of the family Asteraceae that has spread in Vojvodina since the 1960s. The weed is currently most frequent in ruderal localities but it has also infested field crops over the past ten years. As part of an initial study of characteristics of giant sumpweed, we examined its spreading in the region of Vojvodina. Rural ruderal areas were included, as well as maize, sugar beet, sunflower and soybean crops. Giant sumpweed was detected in 51 localities in Vojvodina during investigation that was conducted from 2002 to 2006. It was most frequent in the region of Srem, i.e. in 24 localities, as well as in 17 localities in Bačka and 10 in Banat. Giant sumpweed predominantly occurs in ruderal habitats and has established itself well in ruderal floristic structures (24 localities), but it has also adapted well to the floristic structures of maize (10 localities), sugar beet (7 localities), sunflower (7 localities) and soybean (4 localities) crops. None of the investigated localities had a pure population with 100% presence of giant sumpweed but the species was one of 17 weeds that were found in all localities, which indicates that it has adapted well in Vojvodina and has a considerable potential for spreading.
AB  - Iva (Iva xanthifolia Nutt.) je nova invazivna vrsta korova iz porodice Asteraceae koja se u Vojvodini proširila šezdesetih godina prošlog veka. Trenutno se ova korovska vrsta najčešće sreće na ruderalnim lokalitetima, ali je tokom proteklih deset godina registrovana i u ratarskim usevima. U okviru preliminarnog ispitivanja osobina ive, proučavali smo njeno širenje u regionu Vojvodine. Proučavanja su obavljena u ruralnim ruderalnim područjima, kao i u usevima kukuruza, šećerne repe, suncokreta i soje. Tokom ispitivanja, koje je trajalo od 2002. do 2006. godine, iva je registrovana na 51 lokaciji u Vojvodini. Najrasprostranjenija je u regionu Srema, na 24 lokacija, a nađena je i na 17 lokacija u Bačkoj i 10 u Banatu. Iva je najrasprostranjenija na ruderalnim staništima i dobro se uklopila u ruderalne florističke strukture (24 lokacija), ali se prilagodila i florističkim strukturama useva kukuruza (10 lokaliteta), šećerne repe (7 lokaliteta), suncokreta (7 lokaliteta) i soje (4 lokaliteta). Mada ni na jednom lokalitetu iva nije zabeležena u čistoj populaciji sa 100% prisustva, ona predstavlja jednu od 17 korovskih vrsta koje su nađene na svim lokalitetima, što pokazuje da se dobro prilagodila uslovima Vojvodine i ima značajan potencijal širenja.
PB  - Herbološko društvo Srbije, Beograd
T2  - Acta herbologica
T1  - Presence of the allochthonous invasive weed Iva xanthifolia Nutt. in Vojvodina
T1  - Prisustvo i kvantitativna zastupljenost alohtone invazivne korovske vrste Iva xanthifolia Nutt. na teritoriji Vojvodine
EP  - 125
IS  - 2
SP  - 105
VL  - 16
ER  - 

@article{
author = "Marisavljević, Dragana and Stojanović, Slobodanka and Pavlović, Danijela and Pfaf-Dolovac, Erika",
year = "2007",
abstract = "Giant sumpweed (Iva xanthifolia Nutt.) is a newly-introduced invasive weed of the family Asteraceae that has spread in Vojvodina since the 1960s. The weed is currently most frequent in ruderal localities but it has also infested field crops over the past ten years. As part of an initial study of characteristics of giant sumpweed, we examined its spreading in the region of Vojvodina. Rural ruderal areas were included, as well as maize, sugar beet, sunflower and soybean crops. Giant sumpweed was detected in 51 localities in Vojvodina during investigation that was conducted from 2002 to 2006. It was most frequent in the region of Srem, i.e. in 24 localities, as well as in 17 localities in Bačka and 10 in Banat. Giant sumpweed predominantly occurs in ruderal habitats and has established itself well in ruderal floristic structures (24 localities), but it has also adapted well to the floristic structures of maize (10 localities), sugar beet (7 localities), sunflower (7 localities) and soybean (4 localities) crops. None of the investigated localities had a pure population with 100% presence of giant sumpweed but the species was one of 17 weeds that were found in all localities, which indicates that it has adapted well in Vojvodina and has a considerable potential for spreading., Iva (Iva xanthifolia Nutt.) je nova invazivna vrsta korova iz porodice Asteraceae koja se u Vojvodini proširila šezdesetih godina prošlog veka. Trenutno se ova korovska vrsta najčešće sreće na ruderalnim lokalitetima, ali je tokom proteklih deset godina registrovana i u ratarskim usevima. U okviru preliminarnog ispitivanja osobina ive, proučavali smo njeno širenje u regionu Vojvodine. Proučavanja su obavljena u ruralnim ruderalnim područjima, kao i u usevima kukuruza, šećerne repe, suncokreta i soje. Tokom ispitivanja, koje je trajalo od 2002. do 2006. godine, iva je registrovana na 51 lokaciji u Vojvodini. Najrasprostranjenija je u regionu Srema, na 24 lokacija, a nađena je i na 17 lokacija u Bačkoj i 10 u Banatu. Iva je najrasprostranjenija na ruderalnim staništima i dobro se uklopila u ruderalne florističke strukture (24 lokacija), ali se prilagodila i florističkim strukturama useva kukuruza (10 lokaliteta), šećerne repe (7 lokaliteta), suncokreta (7 lokaliteta) i soje (4 lokaliteta). Mada ni na jednom lokalitetu iva nije zabeležena u čistoj populaciji sa 100% prisustva, ona predstavlja jednu od 17 korovskih vrsta koje su nađene na svim lokalitetima, što pokazuje da se dobro prilagodila uslovima Vojvodine i ima značajan potencijal širenja.",
publisher = "Herbološko društvo Srbije, Beograd",
journal = "Acta herbologica",
title = "Presence of the allochthonous invasive weed Iva xanthifolia Nutt. in Vojvodina, Prisustvo i kvantitativna zastupljenost alohtone invazivne korovske vrste Iva xanthifolia Nutt. na teritoriji Vojvodine",
pages = "125-105",
number = "2",
volume = "16"
}

Marisavljević, D., Stojanović, S., Pavlović, D.,& Pfaf-Dolovac, E.. (2007). Presence of the allochthonous invasive weed Iva xanthifolia Nutt. in Vojvodina. in Acta herbologica
Herbološko društvo Srbije, Beograd., 16(2), 105-125.

Marisavljević D, Stojanović S, Pavlović D, Pfaf-Dolovac E. Presence of the allochthonous invasive weed Iva xanthifolia Nutt. in Vojvodina. in Acta herbologica. 2007;16(2):105-125..

Marisavljević, Dragana, Stojanović, Slobodanka, Pavlović, Danijela, Pfaf-Dolovac, Erika, "Presence of the allochthonous invasive weed Iva xanthifolia Nutt. in Vojvodina" in Acta herbologica, 16, no. 2 (2007):105-125.

TY  - JOUR
AU  - Marisavljević, Dragana
AU  - Pavlović, Danijela
AU  - Pfaf-Dolovac, Erika
PY  - 2007
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/46
AB  - This paper discusses problem of current weed species in oilseed rape crops, their influence on growing and developing of crop, yield, harvesting and quality of oil.
AB  - U radu su prikazane korovske vrste koje se javljaju u usevu uljane repice i njihov uticaj na rast i razvoj useva, žetvu, prinos i kvalitet ulja.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Weeds flora in oilseed rape crops
T1  - Korovska flora useva uljane repice
EP  - 467
IS  - 4
SP  - 464
VL  - 35
ER  - 

@article{
author = "Marisavljević, Dragana and Pavlović, Danijela and Pfaf-Dolovac, Erika",
year = "2007",
abstract = "This paper discusses problem of current weed species in oilseed rape crops, their influence on growing and developing of crop, yield, harvesting and quality of oil., U radu su prikazane korovske vrste koje se javljaju u usevu uljane repice i njihov uticaj na rast i razvoj useva, žetvu, prinos i kvalitet ulja.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Weeds flora in oilseed rape crops, Korovska flora useva uljane repice",
pages = "467-464",
number = "4",
volume = "35"
}

Marisavljević, D., Pavlović, D.,& Pfaf-Dolovac, E.. (2007). Weeds flora in oilseed rape crops. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 35(4), 464-467.

Marisavljević D, Pavlović D, Pfaf-Dolovac E. Weeds flora in oilseed rape crops. in Biljni lekar. 2007;35(4):464-467..

Marisavljević, Dragana, Pavlović, Danijela, Pfaf-Dolovac, Erika, "Weeds flora in oilseed rape crops" in Biljni lekar, 35, no. 4 (2007):464-467.