TY  - JOUR
AU  - Radunović, Dragana
AU  - Gavrilović, Veljko
AU  - Gašić, Katarina
AU  - Paunović, M.
AU  - Stojšin, Vera
AU  - Grahovac, M.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/484
AB  - In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro
EP  - 203
IS  - 1
SP  - 197
VL  - 99
DO  - 10.4454/jpp.v99i1.3853
ER  - 

@article{
author = "Radunović, Dragana and Gavrilović, Veljko and Gašić, Katarina and Paunović, M. and Stojšin, Vera and Grahovac, M.",
year = "2017",
abstract = "In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro",
pages = "203-197",
number = "1",
volume = "99",
doi = "10.4454/jpp.v99i1.3853"
}

Radunović, D., Gavrilović, V., Gašić, K., Paunović, M., Stojšin, V.,& Grahovac, M.. (2017). Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology
Edizioni Ets, Pisa., 99(1), 197-203.
https://doi.org/10.4454/jpp.v99i1.3853

Radunović D, Gavrilović V, Gašić K, Paunović M, Stojšin V, Grahovac M. Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology. 2017;99(1):197-203.
doi:10.4454/jpp.v99i1.3853 .

Radunović, Dragana, Gavrilović, Veljko, Gašić, Katarina, Paunović, M., Stojšin, Vera, Grahovac, M., "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro" in Journal of Plant Pathology, 99, no. 1 (2017):197-203,
https://doi.org/10.4454/jpp.v99i1.3853 . .