TY  - JOUR
AU  - Nikolić, I.
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Stojšin, Vera
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/553
AB  - Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet
EP  - 1207
IS  - 5
SP  - 1194
VL  - 67
DO  - 10.1111/ppa.12831
ER  - 

@article{
author = "Nikolić, I. and Stanković, Slaviša and Dimkić, Ivica and Berić, Tanja and Stojšin, Vera and Janse, Jaap and Popović, Tatjana",
year = "2018",
abstract = "Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet",
pages = "1207-1194",
number = "5",
volume = "67",
doi = "10.1111/ppa.12831"
}

Nikolić, I., Stanković, S., Dimkić, I., Berić, T., Stojšin, V., Janse, J.,& Popović, T.. (2018). Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology
Wiley, Hoboken., 67(5), 1194-1207.
https://doi.org/10.1111/ppa.12831

Nikolić I, Stanković S, Dimkić I, Berić T, Stojšin V, Janse J, Popović T. Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology. 2018;67(5):1194-1207.
doi:10.1111/ppa.12831 .

Nikolić, I., Stanković, Slaviša, Dimkić, Ivica, Berić, Tanja, Stojšin, Vera, Janse, Jaap, Popović, Tatjana, "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet" in Plant Pathology, 67, no. 5 (2018):1194-1207,
https://doi.org/10.1111/ppa.12831 . .

TY  - JOUR
AU  - Balaž, Jelica
AU  - Ognjanov, Vladislav
AU  - Keserović, Zoran
AU  - Šućur, Aleksandra
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/490
AB  - This study presents the results of a multiple-year evaluation (1991-1996; 2005-2007) of susceptibility of more than 100 apple cultivars to Venturia inaequalis, Podosphaera leucotricha and Erwinia amylovora under agroecological conditions existing in Serbia. Some of the most popular cultivars were found highly susceptible to V. inaequalis (Cripps Pink, Mutsu, Gloster 69, Wellspur); while Golden Delicious, Richared, Gala, Čačanska pozna, Čadel and Jonagold were susceptible; Idared, Granny Smith and Jonathan moderately susceptible; Lord Lamburne and London Pepping, as well as several autochthonous cultivars were moderately resistant; and a group of resistant cultivars included Prima, Priscilla, Williams Pride, Dayton, Enterprise, Gold Rush, Golden Orange, many of the Re-cultivars (Germany), as well as Baujade, Selena, Dukát, Produkta, Topaz, some older cvs. (Worcester Pearmain, Merton Worcester, James Grieve, Akane, Astilish, Astrachan Red and Discovery), some Co-op selections (USA) and NS hybrids (Serbia). Regarding powdery mildew, Idared and Jonathan were highly susceptible; Gala, Akane, Jonagold, Priscilla, Mutsu, Čačanska pozna, Golden Delicious, Granny Smith, Čadel and GoldRush were susceptible; Wellspur, Astrachan Red, Richared, Jonadel, Dayton and several autochthonous cultivars were moderately susceptible; Lord Lamburne, Astlisch, Prima, Champagne Reinette, Discovery and many autochthonous cultivars were moderately resistant; while most Re-cvs. (Germany), several cultivars from the Czech Republic, some selections from the USA and UK and most NS hybrids (Serbia) were resistant. Also, some cultivars showed variable susceptibility depending on location (Williams Pride, Gloster 69, Baujade and Produkta). E. amylovora was observed only in 2007 and at relatively low intensity (up to 12% infection). The highest disease severity was observed on cv. Elstar, then Granny Smith, Idared and Jonagored; while the lowest was found on Red Chief and Hapke apple trees.
AB  - Ovaj rad prikazuje rezultate višegodišnjeg proučavanja (1991-1996; 2005-2007) osetljivosti više od 100 sorti jabuke prema patogenima Venturia inaequalis, Podosphaera leucotricha i Erwinia amylovora u agroekološkim uslovima Srbije. Neke od dobro poznatih sorti su visoko osetljive prema V. inaequalis (Cripps Pink, Mutsu, Gloster 69, Wellspur); osetljive su Golden Delicious, Richared, Gala, Čačanska pozna, Čadel i Jonagold; umereno osetljive su Idared, Granny Smith i Jonathan; umereno otporne su Lord Lamburne i London Pepping, kao i nekoliko autohtonih sorti; grupa otpornih uključuje sorte Prima, Priscilla, Williams Pride, Dayton, Enterprise, Gold Rush, Golden Orange, mnoge Re-sorte (Nemačka), Baujade, Selena, Dukát, Produkta, Topaz, neke starije sorte (Worcester Pearmain, Merton Worcester, James Grieve, Akane, Astilish, Astrachan Red i Discovery), neke Co-op selekcije (USA) i NS hibridi (Srbija). Prema pepelnici vrlo osetljive su bile sorte Idared i Jonathan; osetljive Gala, Akane, Jonagold, Priscilla, Mutsu, Čačanska pozna, Golden Delicious, Granny Smith, Čadel i GoldRush; umereno osetljive Wellspur, Astrachan Red, Richared, Jonadel, Dayton i nekoliko autohtonih sorti; umereno otporne su Lord Lamburne, Astlisch, Prima, Champagne Reinette, Discovery i mnoge autohtone sorte; i otporne su Re-sorte, nekoliko sorti iz Češke Republike, neke selekcije poreklom iz USA i UK i većina NS hibrida. Takođe, neke sorte su i varirale u osetljivosti (Williams Pride, Gloster 69, Baujade i Produkta). E. amylovora je beležena samo 2007. godine sa relativno malim intenzitetom bolesti (infekcija do 12%). Najveći intenzitet bolesti je primećen kod sorte Elstar, zatim slede sorte Granny Smith, Idared, Jonagored; a najmanji intenzitet je bio kod sorti Red Chief i Hapke.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Evaluation of reactions of commercial and autochthonous apple cultivars to common diseases in Serbia under natural infection
T1  - Proučavanje osetljivosti komercijalnih i autohtonih sorti jabuke prema najznačajnijim bolestima u Srbiji u uslovima spontane zaraze
EP  - 172
IS  - 3-4
SP  - 157
VL  - 32
DO  - 10.2298/PIF1704157B
ER  - 

@article{
author = "Balaž, Jelica and Ognjanov, Vladislav and Keserović, Zoran and Šućur, Aleksandra and Janse, Jaap and Popović, Tatjana",
year = "2017",
abstract = "This study presents the results of a multiple-year evaluation (1991-1996; 2005-2007) of susceptibility of more than 100 apple cultivars to Venturia inaequalis, Podosphaera leucotricha and Erwinia amylovora under agroecological conditions existing in Serbia. Some of the most popular cultivars were found highly susceptible to V. inaequalis (Cripps Pink, Mutsu, Gloster 69, Wellspur); while Golden Delicious, Richared, Gala, Čačanska pozna, Čadel and Jonagold were susceptible; Idared, Granny Smith and Jonathan moderately susceptible; Lord Lamburne and London Pepping, as well as several autochthonous cultivars were moderately resistant; and a group of resistant cultivars included Prima, Priscilla, Williams Pride, Dayton, Enterprise, Gold Rush, Golden Orange, many of the Re-cultivars (Germany), as well as Baujade, Selena, Dukát, Produkta, Topaz, some older cvs. (Worcester Pearmain, Merton Worcester, James Grieve, Akane, Astilish, Astrachan Red and Discovery), some Co-op selections (USA) and NS hybrids (Serbia). Regarding powdery mildew, Idared and Jonathan were highly susceptible; Gala, Akane, Jonagold, Priscilla, Mutsu, Čačanska pozna, Golden Delicious, Granny Smith, Čadel and GoldRush were susceptible; Wellspur, Astrachan Red, Richared, Jonadel, Dayton and several autochthonous cultivars were moderately susceptible; Lord Lamburne, Astlisch, Prima, Champagne Reinette, Discovery and many autochthonous cultivars were moderately resistant; while most Re-cvs. (Germany), several cultivars from the Czech Republic, some selections from the USA and UK and most NS hybrids (Serbia) were resistant. Also, some cultivars showed variable susceptibility depending on location (Williams Pride, Gloster 69, Baujade and Produkta). E. amylovora was observed only in 2007 and at relatively low intensity (up to 12% infection). The highest disease severity was observed on cv. Elstar, then Granny Smith, Idared and Jonagored; while the lowest was found on Red Chief and Hapke apple trees., Ovaj rad prikazuje rezultate višegodišnjeg proučavanja (1991-1996; 2005-2007) osetljivosti više od 100 sorti jabuke prema patogenima Venturia inaequalis, Podosphaera leucotricha i Erwinia amylovora u agroekološkim uslovima Srbije. Neke od dobro poznatih sorti su visoko osetljive prema V. inaequalis (Cripps Pink, Mutsu, Gloster 69, Wellspur); osetljive su Golden Delicious, Richared, Gala, Čačanska pozna, Čadel i Jonagold; umereno osetljive su Idared, Granny Smith i Jonathan; umereno otporne su Lord Lamburne i London Pepping, kao i nekoliko autohtonih sorti; grupa otpornih uključuje sorte Prima, Priscilla, Williams Pride, Dayton, Enterprise, Gold Rush, Golden Orange, mnoge Re-sorte (Nemačka), Baujade, Selena, Dukát, Produkta, Topaz, neke starije sorte (Worcester Pearmain, Merton Worcester, James Grieve, Akane, Astilish, Astrachan Red i Discovery), neke Co-op selekcije (USA) i NS hibridi (Srbija). Prema pepelnici vrlo osetljive su bile sorte Idared i Jonathan; osetljive Gala, Akane, Jonagold, Priscilla, Mutsu, Čačanska pozna, Golden Delicious, Granny Smith, Čadel i GoldRush; umereno osetljive Wellspur, Astrachan Red, Richared, Jonadel, Dayton i nekoliko autohtonih sorti; umereno otporne su Lord Lamburne, Astlisch, Prima, Champagne Reinette, Discovery i mnoge autohtone sorte; i otporne su Re-sorte, nekoliko sorti iz Češke Republike, neke selekcije poreklom iz USA i UK i većina NS hibrida. Takođe, neke sorte su i varirale u osetljivosti (Williams Pride, Gloster 69, Baujade i Produkta). E. amylovora je beležena samo 2007. godine sa relativno malim intenzitetom bolesti (infekcija do 12%). Najveći intenzitet bolesti je primećen kod sorte Elstar, zatim slede sorte Granny Smith, Idared, Jonagored; a najmanji intenzitet je bio kod sorti Red Chief i Hapke.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Evaluation of reactions of commercial and autochthonous apple cultivars to common diseases in Serbia under natural infection, Proučavanje osetljivosti komercijalnih i autohtonih sorti jabuke prema najznačajnijim bolestima u Srbiji u uslovima spontane zaraze",
pages = "172-157",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704157B"
}

Balaž, J., Ognjanov, V., Keserović, Z., Šućur, A., Janse, J.,& Popović, T.. (2017). Evaluation of reactions of commercial and autochthonous apple cultivars to common diseases in Serbia under natural infection. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 157-172.
https://doi.org/10.2298/PIF1704157B

Balaž J, Ognjanov V, Keserović Z, Šućur A, Janse J, Popović T. Evaluation of reactions of commercial and autochthonous apple cultivars to common diseases in Serbia under natural infection. in Pesticidi i fitomedicina. 2017;32(3-4):157-172.
doi:10.2298/PIF1704157B .

Balaž, Jelica, Ognjanov, Vladislav, Keserović, Zoran, Šućur, Aleksandra, Janse, Jaap, Popović, Tatjana, "Evaluation of reactions of commercial and autochthonous apple cultivars to common diseases in Serbia under natural infection" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):157-172,
https://doi.org/10.2298/PIF1704157B . .

TY  - JOUR
AU  - Balaž, Jelica
AU  - Ivanović, Žarko
AU  - Davidović, Andrej
AU  - Ilicić, Renata
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/426
AB  - Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)(5) and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia
EP  - 170
IS  - 1
SP  - 164
VL  - 100
DO  - 10.1094/PDIS-03-15-0295-RE
ER  - 

@article{
author = "Balaž, Jelica and Ivanović, Žarko and Davidović, Andrej and Ilicić, Renata and Janse, Jaap and Popović, Tatjana",
year = "2016",
abstract = "Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)(5) and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia",
pages = "170-164",
number = "1",
volume = "100",
doi = "10.1094/PDIS-03-15-0295-RE"
}

Balaž, J., Ivanović, Ž., Davidović, A., Ilicić, R., Janse, J.,& Popović, T.. (2016). Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 164-170.
https://doi.org/10.1094/PDIS-03-15-0295-RE

Balaž J, Ivanović Ž, Davidović A, Ilicić R, Janse J, Popović T. Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia. in Plant Disease. 2016;100(1):164-170.
doi:10.1094/PDIS-03-15-0295-RE .

Balaž, Jelica, Ivanović, Žarko, Davidović, Andrej, Ilicić, Renata, Janse, Jaap, Popović, Tatjana, "Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia" in Plant Disease, 100, no. 1 (2016):164-170,
https://doi.org/10.1094/PDIS-03-15-0295-RE . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Janse, Jaap
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Gavrilović, Veljko
AU  - Fira, Đorđe
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/396
AB  - The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques
EP  - 145
IS  - 1
SP  - 133
VL  - 141
DO  - 10.1007/s10658-014-0531-5
ER  - 

@article{
author = "Ivanović, Žarko and Popović, Tatjana and Janse, Jaap and Kojić, Milan and Stanković, Slaviša and Gavrilović, Veljko and Fira, Đorđe",
year = "2015",
abstract = "The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques",
pages = "145-133",
number = "1",
volume = "141",
doi = "10.1007/s10658-014-0531-5"
}

Ivanović, Ž., Popović, T., Janse, J., Kojić, M., Stanković, S., Gavrilović, V.,& Fira, Đ.. (2015). Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology
Springer, Dordrecht., 141(1), 133-145.
https://doi.org/10.1007/s10658-014-0531-5

Ivanović Ž, Popović T, Janse J, Kojić M, Stanković S, Gavrilović V, Fira Đ. Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology. 2015;141(1):133-145.
doi:10.1007/s10658-014-0531-5 .

Ivanović, Žarko, Popović, Tatjana, Janse, Jaap, Kojić, Milan, Stanković, Slaviša, Gavrilović, Veljko, Fira, Đorđe, "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques" in European Journal of Plant Pathology, 141, no. 1 (2015):133-145,
https://doi.org/10.1007/s10658-014-0531-5 . .