TY  - JOUR
AU  - Stanisavljević, Rade
AU  - Velijević, Nataša
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Aleksić, Goran
AU  - Trkulja, Nenad
AU  - Knezević, Jasmina
AU  - Dodig, Dejan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/526
AB  - In many regions worldwide, vetch is an important forage crops for the production of fibrous feed. Vetch seed available on the south-eastern Europe market often differ in the size, colour and age, hence seed quality and seedling vigour are questionable. Seed samples of two vetch varieties were drawn from three lots during two years. According to these samples, seeds were classified into groups of three sizes and three colours. The effect of the seed size and colour on seed quality and seedling vigour was estimated immediately after harvest, and then nine, 21 and 33 months after harvest. The determined effects of seed size, colour and age, as well as of their interactions on seed quality and seedling vigour were significant. On the other hand, years, varieties, lots and their interactions did not significantly affect seed quality and seedling vigour. There were no significant differences in seed quality and seedling vigour over nine, 21 and 33 month storage periods, but the ageing test showed the significant differences among the duration of storage. Germination and vigour of seedlings were significantly higher in large seeds. Pale seeds had better quality after nine and 21 month storage period (time when the seed in the second year after harvesting should be sown), while seed quality was greater in dark seeds after 33 months of storage (time when the seed in the third year after harvesting should be sown). (C) 2018 Friends Science Publishers
PB  - Friends Science Publ, Faisalabad
T2  - International Journal of Agriculture and Biology
T1  - Seed Quality of Vetch (Vicia sativa) affected by Different Seed Colors and Sizes after Various Storage Periods
EP  - 2660
IS  - 12
SP  - 2655
VL  - 20
DO  - 10.17957/IJAB/15.0806
ER  - 

@article{
author = "Stanisavljević, Rade and Velijević, Nataša and Štrbanović, Ratibor and Poštić, Dobrivoj and Aleksić, Goran and Trkulja, Nenad and Knezević, Jasmina and Dodig, Dejan",
year = "2018",
abstract = "In many regions worldwide, vetch is an important forage crops for the production of fibrous feed. Vetch seed available on the south-eastern Europe market often differ in the size, colour and age, hence seed quality and seedling vigour are questionable. Seed samples of two vetch varieties were drawn from three lots during two years. According to these samples, seeds were classified into groups of three sizes and three colours. The effect of the seed size and colour on seed quality and seedling vigour was estimated immediately after harvest, and then nine, 21 and 33 months after harvest. The determined effects of seed size, colour and age, as well as of their interactions on seed quality and seedling vigour were significant. On the other hand, years, varieties, lots and their interactions did not significantly affect seed quality and seedling vigour. There were no significant differences in seed quality and seedling vigour over nine, 21 and 33 month storage periods, but the ageing test showed the significant differences among the duration of storage. Germination and vigour of seedlings were significantly higher in large seeds. Pale seeds had better quality after nine and 21 month storage period (time when the seed in the second year after harvesting should be sown), while seed quality was greater in dark seeds after 33 months of storage (time when the seed in the third year after harvesting should be sown). (C) 2018 Friends Science Publishers",
publisher = "Friends Science Publ, Faisalabad",
journal = "International Journal of Agriculture and Biology",
title = "Seed Quality of Vetch (Vicia sativa) affected by Different Seed Colors and Sizes after Various Storage Periods",
pages = "2660-2655",
number = "12",
volume = "20",
doi = "10.17957/IJAB/15.0806"
}

Stanisavljević, R., Velijević, N., Štrbanović, R., Poštić, D., Aleksić, G., Trkulja, N., Knezević, J.,& Dodig, D.. (2018). Seed Quality of Vetch (Vicia sativa) affected by Different Seed Colors and Sizes after Various Storage Periods. in International Journal of Agriculture and Biology
Friends Science Publ, Faisalabad., 20(12), 2655-2660.
https://doi.org/10.17957/IJAB/15.0806

Stanisavljević R, Velijević N, Štrbanović R, Poštić D, Aleksić G, Trkulja N, Knezević J, Dodig D. Seed Quality of Vetch (Vicia sativa) affected by Different Seed Colors and Sizes after Various Storage Periods. in International Journal of Agriculture and Biology. 2018;20(12):2655-2660.
doi:10.17957/IJAB/15.0806 .

Stanisavljević, Rade, Velijević, Nataša, Štrbanović, Ratibor, Poštić, Dobrivoj, Aleksić, Goran, Trkulja, Nenad, Knezević, Jasmina, Dodig, Dejan, "Seed Quality of Vetch (Vicia sativa) affected by Different Seed Colors and Sizes after Various Storage Periods" in International Journal of Agriculture and Biology, 20, no. 12 (2018):2655-2660,
https://doi.org/10.17957/IJAB/15.0806 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Perović, Tatjana
AU  - Popović, Tatjana
AU  - Blagojević, Jovana
AU  - Trkulja, Nenad
AU  - Hrnčić, Snježana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/474
AB  - Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gapl gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gapl gene sequences showed that P syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P syringae control.
PB  - Hanrimwon Publishing Company
T2  - Plant Pathology Journal
T1  - Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro
EP  - 33
IS  - 1
SP  - 21
VL  - 33
DO  - 10.5423/PPJ.OA.08.2016.0161
ER  - 

@article{
author = "Ivanović, Žarko and Perović, Tatjana and Popović, Tatjana and Blagojević, Jovana and Trkulja, Nenad and Hrnčić, Snježana",
year = "2017",
abstract = "Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gapl gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gapl gene sequences showed that P syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P syringae control.",
publisher = "Hanrimwon Publishing Company",
journal = "Plant Pathology Journal",
title = "Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro",
pages = "33-21",
number = "1",
volume = "33",
doi = "10.5423/PPJ.OA.08.2016.0161"
}

Ivanović, Ž., Perović, T., Popović, T., Blagojević, J., Trkulja, N.,& Hrnčić, S.. (2017). Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro. in Plant Pathology Journal
Hanrimwon Publishing Company., 33(1), 21-33.
https://doi.org/10.5423/PPJ.OA.08.2016.0161

Ivanović Ž, Perović T, Popović T, Blagojević J, Trkulja N, Hrnčić S. Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro. in Plant Pathology Journal. 2017;33(1):21-33.
doi:10.5423/PPJ.OA.08.2016.0161 .

Ivanović, Žarko, Perović, Tatjana, Popović, Tatjana, Blagojević, Jovana, Trkulja, Nenad, Hrnčić, Snježana, "Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro" in Plant Pathology Journal, 33, no. 1 (2017):21-33,
https://doi.org/10.5423/PPJ.OA.08.2016.0161 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Khan, Mohamed F. R.
AU  - Secor, Gary A.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/475
AB  - Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides
EP  - 910
IS  - 4
SP  - 895
VL  - 149
DO  - 10.1007/s10658-017-1239-0
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Khan, Mohamed F. R. and Secor, Gary A.",
year = "2017",
abstract = "Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides",
pages = "910-895",
number = "4",
volume = "149",
doi = "10.1007/s10658-017-1239-0"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J., Toševski, I., Khan, M. F. R.,& Secor, G. A.. (2017). Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology
Springer, Dordrecht., 149(4), 895-910.
https://doi.org/10.1007/s10658-017-1239-0

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I, Khan MFR, Secor GA. Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology. 2017;149(4):895-910.
doi:10.1007/s10658-017-1239-0 .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Khan, Mohamed F. R., Secor, Gary A., "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides" in European Journal of Plant Pathology, 149, no. 4 (2017):895-910,
https://doi.org/10.1007/s10658-017-1239-0 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Milosavljević, Anja
AU  - Bosković, J.
AU  - Jović, Jelena
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/455
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia
EP  - 221
IS  - 1
SP  - 221
VL  - 100
DO  - 10.1094/PDIS-02-15-0209-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Pfaf-Dolovac, Erika and Milosavljević, Anja and Bosković, J. and Jović, Jelena and Mitrović, Milana and Toševski, Ivo",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia",
pages = "221-221",
number = "1",
volume = "100",
doi = "10.1094/PDIS-02-15-0209-PDN"
}

Trkulja, N., Pfaf-Dolovac, E., Milosavljević, A., Bosković, J., Jović, J., Mitrović, M.,& Toševski, I.. (2016). First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 221-221.
https://doi.org/10.1094/PDIS-02-15-0209-PDN

Trkulja N, Pfaf-Dolovac E, Milosavljević A, Bosković J, Jović J, Mitrović M, Toševski I. First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia. in Plant Disease. 2016;100(1):221-221.
doi:10.1094/PDIS-02-15-0209-PDN .

Trkulja, Nenad, Pfaf-Dolovac, Erika, Milosavljević, Anja, Bosković, J., Jović, Jelena, Mitrović, Milana, Toševski, Ivo, "First Report of QoI Resistance in Botrytis cinerea Isolates Causing Gray Mold in Strawberry Fields in Serbia" in Plant Disease, 100, no. 1 (2016):221-221,
https://doi.org/10.1094/PDIS-02-15-0209-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Stanisavljević, Rade
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Bosković, Jelena
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/416
AB  - The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management
EP  - 87
SP  - 80
VL  - 75
DO  - 10.1016/j.cropro.2015.05.017
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Stanisavljević, Rade and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Bosković, Jelena",
year = "2015",
abstract = "The emergence of Cercospora beticola populations that are resistant to benzimidazoles (MBC) and demethylation-inhibiting fungicides (DMI) has been recently reported in Serbia and has resulted in a reduced efficacy of fungicides in controlling Cercospora leaf spot (CLS). Between 2008 and 2011, using a discriminatory concentration method in sugar beet fields in two separate regions of Serbia, we determined that 93.3%-98.6% of collected C. bed cola isolates were resistant to MBCs, whereas 6.2%-42.4% were resistant to DMI fungicides. At the same localities, field trials were conducted to investigate the impact of resistant C. beticola populations on disease management From the MBC group of fungicides, both thiophanate methyl and carbendazim failed to suppress the spread of CLS at both of the tested localities. Between 2008 and 2010, DMI fungicides expressed moderate efficacy at a South Banat locality (79.8%-84.6%) whether they were applied individually (flutriafol, epoxiconazole) or in combination with MBCs (epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole). The frequency of resistant isolates in these test trials ranged from 6.2% to 10.9%. In 2011, at the same locality, conditions were observed to change in favor of the occurrence of resistant populations, which comprised up to 18.7% of the population, at the expense of DMI efficacy in CLS management At a Srem locality, the frequency of C. beticola isolates that were resistant to DMIs was high during all four years of field testing (30.5%-42.4%), and the efficacy of these fungicides ranged from 48.4% to 68.0%. A combination of DMI and a protective chlorothalonil had a stable, moderate impact on disease management regardless of the frequency of DMI resistance, whereas a combination of the cyproconazole DMI with trifloxystrobin from the strobilurin group of fungicides expressed the highest efficacy. High correlation coefficient values (r = 0.87) indicated how strongly the frequencies of resistant populations affected disease severity in the trial plots that were treated with carbendazim and thiophanate methyl, as well as in the plots that were treated with flutriafol and epoxiconazole (r = 0.98). In shift sensitivity trials, MBC-resistant C. beticola isolates were found at equally high frequencies both before and after the treatments, indicating a complete loss of efficacy in CLS control. This test revealed the significant impact of multiple DMI applications in terms of increasing the frequency of resistant C. beticola populations following treatments with flutriafol, epoxiconazole, epoxiconazole/carbendazim, thiophanate-methyl/epoxiconazole and flutriafol/chlorothalonil, except when used in combination with trifloxystrobin, in which case CLS suppression was substantially high. (C) 2015 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management",
pages = "87-80",
volume = "75",
doi = "10.1016/j.cropro.2015.05.017"
}

Trkulja, N., Milosavljević, A., Stanisavljević, R., Mitrović, M., Jović, J., Toševski, I.,& Bosković, J.. (2015). Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection
Elsevier Sci Ltd, Oxford., 75, 80-87.
https://doi.org/10.1016/j.cropro.2015.05.017

Trkulja N, Milosavljević A, Stanisavljević R, Mitrović M, Jović J, Toševski I, Bosković J. Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management. in Crop Protection. 2015;75:80-87.
doi:10.1016/j.cropro.2015.05.017 .

Trkulja, Nenad, Milosavljević, Anja, Stanisavljević, Rade, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Bosković, Jelena, "Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management" in Crop Protection, 75 (2015):80-87,
https://doi.org/10.1016/j.cropro.2015.05.017 . .

TY  - JOUR
AU  - Stanisavljević, Rade
AU  - Vučković, Savo
AU  - Štrbanović, Ratibor
AU  - Poštić, Dobrivoj
AU  - Trkulja, Nenad
AU  - Radić, Vojo
AU  - Dodig, Dejan
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/382
AB  - Seeds of three forage plant species, cocksfoot, tall fescue and perennial ryegrass with good amount of dormancy were chemically treated (25%, 50%, 75% and 98% H2SO4) for different durations and exposed to different temperatures (40 degrees C, 50 degrees C, 60 degrees C, 70 degrees C, 80 degrees C and 90 degrees C) for varying periods with an aim to decrease seed dormancy and increase seed germination. Three groups of seeds based on after ripening period of 0, 3 and 8 months were subjected to these treatments. Immediately after harvest, germination of cocksfoot and tall fescue seeds increased by 24%, but only 13% in perennial ryegrass. Three months after harvest it was possible to increase germination by 20% (cocksfoot), 18% (tall fescue) and 6% (perennial ryegrass). Eight months after harvest it was still possible to increase seed germination of cocksfoot and tall fescue by 4-5% whereas, in ryegrass dormancy was completely lost after 8 months storage
PB  - Range Management Soc India, Jhansi
T2  - Range Management and Agroforestry
T1  - Enhancement of seed germination in three grass species using chemical and temperature treatments
EP  - 121
IS  - 2
SP  - 115
VL  - 36
ER  - 

@article{
author = "Stanisavljević, Rade and Vučković, Savo and Štrbanović, Ratibor and Poštić, Dobrivoj and Trkulja, Nenad and Radić, Vojo and Dodig, Dejan",
year = "2015",
abstract = "Seeds of three forage plant species, cocksfoot, tall fescue and perennial ryegrass with good amount of dormancy were chemically treated (25%, 50%, 75% and 98% H2SO4) for different durations and exposed to different temperatures (40 degrees C, 50 degrees C, 60 degrees C, 70 degrees C, 80 degrees C and 90 degrees C) for varying periods with an aim to decrease seed dormancy and increase seed germination. Three groups of seeds based on after ripening period of 0, 3 and 8 months were subjected to these treatments. Immediately after harvest, germination of cocksfoot and tall fescue seeds increased by 24%, but only 13% in perennial ryegrass. Three months after harvest it was possible to increase germination by 20% (cocksfoot), 18% (tall fescue) and 6% (perennial ryegrass). Eight months after harvest it was still possible to increase seed germination of cocksfoot and tall fescue by 4-5% whereas, in ryegrass dormancy was completely lost after 8 months storage",
publisher = "Range Management Soc India, Jhansi",
journal = "Range Management and Agroforestry",
title = "Enhancement of seed germination in three grass species using chemical and temperature treatments",
pages = "121-115",
number = "2",
volume = "36"
}

Stanisavljević, R., Vučković, S., Štrbanović, R., Poštić, D., Trkulja, N., Radić, V.,& Dodig, D.. (2015). Enhancement of seed germination in three grass species using chemical and temperature treatments. in Range Management and Agroforestry
Range Management Soc India, Jhansi., 36(2), 115-121.

Stanisavljević R, Vučković S, Štrbanović R, Poštić D, Trkulja N, Radić V, Dodig D. Enhancement of seed germination in three grass species using chemical and temperature treatments. in Range Management and Agroforestry. 2015;36(2):115-121..

Stanisavljević, Rade, Vučković, Savo, Štrbanović, Ratibor, Poštić, Dobrivoj, Trkulja, Nenad, Radić, Vojo, Dodig, Dejan, "Enhancement of seed germination in three grass species using chemical and temperature treatments" in Range Management and Agroforestry, 36, no. 2 (2015):115-121.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Živković, Svetlana
AU  - Popović, Tatjana
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/397
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.
EP  - 1036
IS  - 7
SP  - 1035
VL  - 99
DO  - 10.1094/PDIS-10-14-1096-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Živković, Svetlana and Popović, Tatjana and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.",
pages = "1036-1035",
number = "7",
volume = "99",
doi = "10.1094/PDIS-10-14-1096-PDN"
}

Trkulja, N., Milosavljević, A., Živković, S., Popović, T., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(7), 1035-1036.
https://doi.org/10.1094/PDIS-10-14-1096-PDN

Trkulja N, Milosavljević A, Živković S, Popović T, Mitrović M, Jović J, Toševski I. First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia.. in Plant Disease. 2015;99(7):1035-1036.
doi:10.1094/PDIS-10-14-1096-PDN .

Trkulja, Nenad, Milosavljević, Anja, Živković, Svetlana, Popović, Tatjana, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora violae Infecting the Garden Violet Viola odorata in Serbia." in Plant Disease, 99, no. 7 (2015):1035-1036,
https://doi.org/10.1094/PDIS-10-14-1096-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Trkulja, Nenad
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/398
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.
EP  - 1275
IS  - 9
SP  - 1274
VL  - 99
DO  - 10.1094/PDIS-12-14-1261-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Trkulja, Nenad and Popović, Tatjana and Ivanović, Žarko and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.",
pages = "1275-1274",
number = "9",
volume = "99",
doi = "10.1094/PDIS-12-14-1261-PDN"
}

Milosavljević, A., Trkulja, N., Popović, T., Ivanović, Ž., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(9), 1274-1275.
https://doi.org/10.1094/PDIS-12-14-1261-PDN

Milosavljević A, Trkulja N, Popović T, Ivanović Ž, Mitrović M, Jović J, Toševski I. First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia.. in Plant Disease. 2015;99(9):1274-1275.
doi:10.1094/PDIS-12-14-1261-PDN .

Milosavljević, Anja, Trkulja, Nenad, Popović, Tatjana, Ivanović, Žarko, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Thielaviopsis thielavioides, A Causal Agent of Postharvest Blackening on Daucus carota in Serbia." in Plant Disease, 99, no. 9 (2015):1274-1275,
https://doi.org/10.1094/PDIS-12-14-1261-PDN . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/404
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia
EP  - 1645
IS  - 11
SP  - 1645
VL  - 99
DO  - 10.1094/PDIS-03-15-0251-PDN
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia",
pages = "1645-1645",
number = "11",
volume = "99",
doi = "10.1094/PDIS-03-15-0251-PDN"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J.,& Toševski, I.. (2015). First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(11), 1645-1645.
https://doi.org/10.1094/PDIS-03-15-0251-PDN

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I. First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia. in Plant Disease. 2015;99(11):1645-1645.
doi:10.1094/PDIS-03-15-0251-PDN .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, "First Report of Cercospora armoraciae, Causal Agent of Cercospora Leaf Spot, on Horseradish in Serbia" in Plant Disease, 99, no. 11 (2015):1645-1645,
https://doi.org/10.1094/PDIS-03-15-0251-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Ignjatov, Maja
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/415
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia
EP  - 724
IS  - 5
SP  - 724
VL  - 99
DO  - 10.1094/PDIS-11-14-1212-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Trkulja, Nenad and Milosavljević, Anja and Ignjatov, Maja",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia",
pages = "724-724",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1212-PDN"
}

Popović, T., Ivanović, Ž., Trkulja, N., Milosavljević, A.,& Ignjatov, M.. (2015). First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 724-724.
https://doi.org/10.1094/PDIS-11-14-1212-PDN

Popović T, Ivanović Ž, Trkulja N, Milosavljević A, Ignjatov M. First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease. 2015;99(5):724-724.
doi:10.1094/PDIS-11-14-1212-PDN .

Popović, Tatjana, Ivanović, Žarko, Trkulja, Nenad, Milosavljević, Anja, Ignjatov, Maja, "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia" in Plant Disease, 99, no. 5 (2015):724-724,
https://doi.org/10.1094/PDIS-11-14-1212-PDN . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Gavrilović, Veljko
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Živković, Svetlana
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/362
AB  - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
AB  - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
T1  - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
EP  - 255
IS  - 4
SP  - 249
VL  - 29
DO  - 10.2298/PIF1404249G
ER  - 

@article{
author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa",
year = "2014",
abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji",
pages = "255-249",
number = "4",
volume = "29",
doi = "10.2298/PIF1404249G"
}

Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 29(4), 249-255.
https://doi.org/10.2298/PIF1404249G

Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255.
doi:10.2298/PIF1404249G .

Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255,
https://doi.org/10.2298/PIF1404249G . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Kuzmanović, Slobodan
AU  - Aleksić, Goran
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/328
AB  - Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%).
AB  - Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion
T1  - Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije
EP  - 32
IS  - 1
SP  - 27
VL  - 65
DO  - 10.5937/zasbilj1401027S
ER  - 

@article{
author = "Stevanović, Miloš and Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Kuzmanović, Slobodan and Aleksić, Goran",
year = "2014",
abstract = "Raspberry cane decay disease are the major problem in its production by reducing yield and lifetime of orchards. This disease cause a several disease-causing agents, and the most important is Didymella applanata, causal agent of raspberry spur blight. With regard to the biology of the pathogen fungicide use postharvest is not efficient enough because pat­hogen can infect raspberry far earlier. Therefore, the aim of this study is testing the efficacy of newer organic fungicides applied before and after the harvest raspberries and mutual comparison thereof on biological efficacy. The results show that the tested fungicides ex­hibited very high efficacy against D. apllanata on the raspberry leaves and the canes (from 83.0 - 96.5%)., Prouzrokovači 'kompleksa sušenja' maline predstavljaju najveći problem u njenoj proizvodnji smanjujući prinose i životni vek zasada. Ovaj kompleks sačinjen je od više prouzrokovača bolesti, a najznačajniji u tom kompleksu je Didymella applanata, prouzrokovač kestenaste pegavosti maline. S obzirom na biologiju patogena tretiranje biljaka tek nakon berbe nije dovoljno efikasno jer patogen u povoljnim uslovima može da zarazi malinu daleko ranije. Cilj rada je ispitivanje efikasnosti novijih organskih fungicida primenjenih pre i posle berbe maline kao i međusobno poređenje njihove biološke efikasnosti. Dobijeni rezultati pokazuju da su ispitivani fungicidi ispoljili veoma visoku efikasnost u suzbijanju D. applanata kako na listu tako i na izdancima maline (od 83.0 - 96.5%).",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion, Suzbijanje Didymella applanata u zasadima maline primenom novijih organskih fungicida tokom vegetacije",
pages = "32-27",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401027S"
}

Stevanović, M., Dolovac, N., Trkulja, N., Milosavljević, A., Kuzmanović, S.,& Aleksić, G.. (2014). Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 27-32.
https://doi.org/10.5937/zasbilj1401027S

Stevanović M, Dolovac N, Trkulja N, Milosavljević A, Kuzmanović S, Aleksić G. Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion. in Zaštita bilja. 2014;65(1):27-32.
doi:10.5937/zasbilj1401027S .

Stevanović, Miloš, Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Kuzmanović, Slobodan, Aleksić, Goran, "Control of Didymella applanata in raspberry orchards using new organic fungicides during vegetacion" in Zaštita bilja, 65, no. 1 (2014):27-32,
https://doi.org/10.5937/zasbilj1401027S . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/325
AB  - C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
AB  - C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and molecular identification of Cercospora apii on celery in Serbia
T1  - Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
EP  - 84
IS  - 2
SP  - 77
VL  - 65
DO  - 10.5937/zasbilj1402077M
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
abstract = "C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank., C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Cercospora apii on celery in Serbia, Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji",
pages = "84-77",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402077M"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(2), 77-84.
https://doi.org/10.5937/zasbilj1402077M

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja. 2014;65(2):77-84.
doi:10.5937/zasbilj1402077M .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "Morphological and molecular identification of Cercospora apii on celery in Serbia" in Zaštita bilja, 65, no. 2 (2014):77-84,
https://doi.org/10.5937/zasbilj1402077M . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Aćimović, Milica
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/304
AB  - During 2013, samples of infected seed grown caraway were collected from commercially grown crops in the experimental field in Mošorin and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a high seed infection 100%, with phytopathogenic fungi of the genus Alternaria, was found. From the infected caraway seed, monosporial cultures, based on morphology, were identified as Alternaria alternata, and their pathogenicity proved on artificially inoculated caraway and parsley seedlings. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ITS1/ITS4 and by amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates CC1 (KP822948), CC2 (KP822949) i CC3 (KP822950) exhibited 100% nucle­otide identity with the sequences of 31 isolates of A. alternata deposited in the GenBank. The presence of a large number of phytopathogenic fungi on caraway seed should be further investigated in order to clarify their relationships and relative significance.
AB  - Tokom 2013. godine, na oglednom polju u Mošorinu, prikupljeni su uzorci zaraženog semena gajenog kima i analizirani na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i visok stepen zaraze fitopatogenim gljivama iz roda Alternaria, 100%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima kima i peršuna, a na osnovu morfoloških svojstava identifikovane su kao Alternaria alternata. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ITS1/ITS4 i amplifikaciju ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata CC1 (KP822948), CC2 (KP822949) i CC3 (KP822950) pokazale su 100% nukleotidne identičnosti sa sekvencama 31 izolata A. alternata deponovanih u GenBank bazi podataka. Prisustvo većeg broja vrsta gljiva na semenu kima, zahteva dalja ispitivanja njihovih međusobnih odnosa i relativnog značaja.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Morphological and molecular identification of Alternaria alternate: Caraway fruits pathogen in Serbia
T1  - Morfološka i molekularna identifikacija izolata Alternaria alternata - patogena plodova kima u Srbiji
EP  - 169
IS  - 4
SP  - 163
VL  - 65
DO  - 10.5937/zasbilj1404163R
ER  - 

@article{
author = "Ristić, Danijela and Aćimović, Milica and Trkulja, Nenad",
year = "2014",
abstract = "During 2013, samples of infected seed grown caraway were collected from commercially grown crops in the experimental field in Mošorin and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a high seed infection 100%, with phytopathogenic fungi of the genus Alternaria, was found. From the infected caraway seed, monosporial cultures, based on morphology, were identified as Alternaria alternata, and their pathogenicity proved on artificially inoculated caraway and parsley seedlings. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ITS1/ITS4 and by amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates CC1 (KP822948), CC2 (KP822949) i CC3 (KP822950) exhibited 100% nucle­otide identity with the sequences of 31 isolates of A. alternata deposited in the GenBank. The presence of a large number of phytopathogenic fungi on caraway seed should be further investigated in order to clarify their relationships and relative significance., Tokom 2013. godine, na oglednom polju u Mošorinu, prikupljeni su uzorci zaraženog semena gajenog kima i analizirani na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i visok stepen zaraze fitopatogenim gljivama iz roda Alternaria, 100%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima kima i peršuna, a na osnovu morfoloških svojstava identifikovane su kao Alternaria alternata. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ITS1/ITS4 i amplifikaciju ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata CC1 (KP822948), CC2 (KP822949) i CC3 (KP822950) pokazale su 100% nukleotidne identičnosti sa sekvencama 31 izolata A. alternata deponovanih u GenBank bazi podataka. Prisustvo većeg broja vrsta gljiva na semenu kima, zahteva dalja ispitivanja njihovih međusobnih odnosa i relativnog značaja.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Alternaria alternate: Caraway fruits pathogen in Serbia, Morfološka i molekularna identifikacija izolata Alternaria alternata - patogena plodova kima u Srbiji",
pages = "169-163",
number = "4",
volume = "65",
doi = "10.5937/zasbilj1404163R"
}

Ristić, D., Aćimović, M.,& Trkulja, N.. (2014). Morphological and molecular identification of Alternaria alternate: Caraway fruits pathogen in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(4), 163-169.
https://doi.org/10.5937/zasbilj1404163R

Ristić D, Aćimović M, Trkulja N. Morphological and molecular identification of Alternaria alternate: Caraway fruits pathogen in Serbia. in Zaštita bilja. 2014;65(4):163-169.
doi:10.5937/zasbilj1404163R .

Ristić, Danijela, Aćimović, Milica, Trkulja, Nenad, "Morphological and molecular identification of Alternaria alternate: Caraway fruits pathogen in Serbia" in Zaštita bilja, 65, no. 4 (2014):163-169,
https://doi.org/10.5937/zasbilj1404163R . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Maširević, Stevan
AU  - Balaž, Jelica
AU  - Pavlović, Snežana
AU  - Oljača, Snežana
AU  - Trkulja, Nenad
AU  - Filipović, Vladimir
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/322
AB  - Fennel (Foeniculum vulgare Mill.) is aromatic plant from Apiaceae family. Fennel fruits (Foeniculi fructus) usually contain 2-6% of essential oil, with trans-anethole which is main component with 60-90%. The fruits are widely used in medicine and nutrition, but also in organic agricultural production. In the world and in our country it was found a large number of diseases, including fungal which are more abundant, than bacterial and phytoplasmas on the fennel. It was determined and the appearance of insects and parasitic flower dodder (Cuscuta sp.).
AB  - Komorač (Foeniculum vulgare Mill.) je aromatična biljka iz familije Apiaceae. Plodovi komorača (Foeniculi fructus) obično sadrže 2-6% etarskog ulja, čija je glavna komponenta trans-anetol koji čini 60-90%. Imaju široku upotrebu u medicini i ishrani, ali i u poljoprivrednoj proizvodnji. Na komoraču je u svetu i kod nas zabeležen veliki broj bolesti, među kojima su gljivične najbrojnije, ali se javljaju i bakterioze i fitoplazme. Utvrđena je i pojava insekata i parazitne cvetnice Cuscuta sp.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Diseases and pests of fennel
T1  - Bolesti i štetočine komorača
EP  - 292
IS  - 4
SP  - 286
VL  - 42
ER  - 

@article{
author = "Aćimović, Milica and Maširević, Stevan and Balaž, Jelica and Pavlović, Snežana and Oljača, Snežana and Trkulja, Nenad and Filipović, Vladimir",
year = "2014",
abstract = "Fennel (Foeniculum vulgare Mill.) is aromatic plant from Apiaceae family. Fennel fruits (Foeniculi fructus) usually contain 2-6% of essential oil, with trans-anethole which is main component with 60-90%. The fruits are widely used in medicine and nutrition, but also in organic agricultural production. In the world and in our country it was found a large number of diseases, including fungal which are more abundant, than bacterial and phytoplasmas on the fennel. It was determined and the appearance of insects and parasitic flower dodder (Cuscuta sp.)., Komorač (Foeniculum vulgare Mill.) je aromatična biljka iz familije Apiaceae. Plodovi komorača (Foeniculi fructus) obično sadrže 2-6% etarskog ulja, čija je glavna komponenta trans-anetol koji čini 60-90%. Imaju široku upotrebu u medicini i ishrani, ali i u poljoprivrednoj proizvodnji. Na komoraču je u svetu i kod nas zabeležen veliki broj bolesti, među kojima su gljivične najbrojnije, ali se javljaju i bakterioze i fitoplazme. Utvrđena je i pojava insekata i parazitne cvetnice Cuscuta sp.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Diseases and pests of fennel, Bolesti i štetočine komorača",
pages = "292-286",
number = "4",
volume = "42"
}

Aćimović, M., Maširević, S., Balaž, J., Pavlović, S., Oljača, S., Trkulja, N.,& Filipović, V.. (2014). Diseases and pests of fennel. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 42(4), 286-292.

Aćimović M, Maširević S, Balaž J, Pavlović S, Oljača S, Trkulja N, Filipović V. Diseases and pests of fennel. in Biljni lekar. 2014;42(4):286-292..

Aćimović, Milica, Maširević, Stevan, Balaž, Jelica, Pavlović, Snežana, Oljača, Snežana, Trkulja, Nenad, Filipović, Vladimir, "Diseases and pests of fennel" in Biljni lekar, 42, no. 4 (2014):286-292.

TY  - JOUR
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - Trkulja, Nenad
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/302
AB  - During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide.
AB  - Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia
T1  - Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji
EP  - 116
IS  - 3
SP  - 111
VL  - 65
DO  - 10.5937/zasbilj1403111R
ER  - 

@article{
author = "Ristić, Danijela and Pavlović, Snežana and Trkulja, Nenad and Pfaf-Dolovac, Erika and Dolovac, Nenad and Starović, Mira",
year = "2014",
abstract = "During 2013, 14 samples of infected seed grown calendula were collected from two commercially grown crops in the localities in Pančevo and Plandište and analyzed for the presence of plant pathogenic fungi. In all samples of seeds, weaker germination and a significant seed infection ranging from 2-8%, with phytopathogenic fungi of the genus Fusarium, was found. From the infected calendula seed, monosporial cultures, based on morphology, were identified as Fusarium verticillioides and Fusarium cf. incarnatum, and their pathogenicity proved on artificially inoculated calendula seedlings. Molecular detection was performed by PCR and amplification of the TEF-1α protein gene. Further studies will include molecular identification of this isolate to the species level, a definitive taxonomic determination of the Serbian isolate and a comparison with other isolates and species of Fusarium genus infecting calendula worldwide., Tokom 2013. godine, na dva lokaliteta komercijalne proizvodnje nevena u Pančevu i Plandištu, prikupljeno je 14 uzoraka zaraženog semena gajenog nevena i analizirano na prisustvo fitopatogenih gljiva. U svim uzorcima semena, ustanovljena je slabija klijavost i značajan stepen zaraze fitopatogenim gljivama iz roda Fusarium, 2-8%. Iz zaraženog semena izolovane su monosporijalne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim klijancima nevena, a na osnovu morfoloških svojstava identifikovane su kao Fusarium verticillioides i Fusarium cf. incarnatum. Molekularna detekcija obavljena je primenom PCR i amplifikacije proteinskog gena TEF-1α. Dalja istraživanja obuhvatiće primenu molekularne identifikacije, do nivoa vrste uz određivanje tačnog taksonomskog mesta izolata iz Srbije patogenih za neven poređenjem sa drugim izolatima i vrstama roda Fusarium spp. u svetu.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia, Fusarium spp. - patogeni semena nevena (Calendula officinalis L.) u Srbiji",
pages = "116-111",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403111R"
}

Ristić, D., Pavlović, S., Trkulja, N., Pfaf-Dolovac, E., Dolovac, N.,& Starović, M.. (2014). Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 111-116.
https://doi.org/10.5937/zasbilj1403111R

Ristić D, Pavlović S, Trkulja N, Pfaf-Dolovac E, Dolovac N, Starović M. Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia. in Zaštita bilja. 2014;65(3):111-116.
doi:10.5937/zasbilj1403111R .

Ristić, Danijela, Pavlović, Snežana, Trkulja, Nenad, Pfaf-Dolovac, Erika, Dolovac, Nenad, Starović, Mira, "Fusarium spp.: Pathogens of calendula seed (Calendula officinalis L.) in Serbia" in Zaštita bilja, 65, no. 3 (2014):111-116,
https://doi.org/10.5937/zasbilj1403111R . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/340
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia
EP  - 1157
IS  - 8
SP  - 1157
VL  - 98
DO  - 10.1094/PDIS-02-14-0135-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia",
pages = "1157-1157",
number = "8",
volume = "98",
doi = "10.1094/PDIS-02-14-0135-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1157-1157.
https://doi.org/10.1094/PDIS-02-14-0135-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease. 2014;98(8):1157-1157.
doi:10.1094/PDIS-02-14-0135-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia" in Plant Disease, 98, no. 8 (2014):1157-1157,
https://doi.org/10.1094/PDIS-02-14-0135-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/341
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.
EP  - 1153
IS  - 8
SP  - 1153
VL  - 98
DO  - 10.1094/PDIS-08-13-0858-PDN
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.",
pages = "1153-1153",
number = "8",
volume = "98",
doi = "10.1094/PDIS-08-13-0858-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1153-1153.
https://doi.org/10.1094/PDIS-08-13-0858-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease. 2014;98(8):1153-1153.
doi:10.1094/PDIS-08-13-0858-PDN .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia." in Plant Disease, 98, no. 8 (2014):1153-1153,
https://doi.org/10.1094/PDIS-08-13-0858-PDN . .

TY  - CONF
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/334
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola
EP  - 120
IS  - 11
SP  - 120
VL  - 104
ER  - 

@conference{
author = "Trkulja, Nenad and Dolovac, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola",
pages = "120-120",
number = "11",
volume = "104"
}

Trkulja, N., Dolovac, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 120-120.

Trkulja N, Dolovac N, Milosavljević A, Pfaf-Dolovac E. Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola. in Phytopathology. 2014;104(11):120-120..

Trkulja, Nenad, Dolovac, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Discriminatory concentration assay to detection of low and high benzimidazole resistant isolates of Cercospora beticola" in Phytopathology, 104, no. 11 (2014):120-120.

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Popović, Tatjana
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/337
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia
EP  - 1272
IS  - 9
SP  - 1272
VL  - 98
DO  - 10.1094/PDIS-01-14-0052-PDN
ER  - 

@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Popović, Tatjana and Dolovac, Nenad and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia",
pages = "1272-1272",
number = "9",
volume = "98",
doi = "10.1094/PDIS-01-14-0052-PDN"
}

Živković, S., Gavrilović, V., Popović, T., Dolovac, N.,& Trkulja, N.. (2014). First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(9), 1272-1272.
https://doi.org/10.1094/PDIS-01-14-0052-PDN

Živković S, Gavrilović V, Popović T, Dolovac N, Trkulja N. First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia. in Plant Disease. 2014;98(9):1272-1272.
doi:10.1094/PDIS-01-14-0052-PDN .

Živković, Svetlana, Gavrilović, Veljko, Popović, Tatjana, Dolovac, Nenad, Trkulja, Nenad, "First Report of Colletotrichum clavatum Causing Quince Anthracnose in Serbia" in Plant Disease, 98, no. 9 (2014):1272-1272,
https://doi.org/10.1094/PDIS-01-14-0052-PDN . .

TY  - CONF
AU  - Dolovac, Nenad
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/335
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management
EP  - 34
IS  - 11
SP  - 34
VL  - 104
ER  - 

@conference{
author = "Dolovac, Nenad and Trkulja, Nenad and Milosavljević, Anja and Pfaf-Dolovac, Erika",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management",
pages = "34-34",
number = "11",
volume = "104"
}

Dolovac, N., Trkulja, N., Milosavljević, A.,& Pfaf-Dolovac, E.. (2014). Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology
Amer Phytopathological Soc, St Paul., 104(11), 34-34.

Dolovac N, Trkulja N, Milosavljević A, Pfaf-Dolovac E. Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management. in Phytopathology. 2014;104(11):34-34..

Dolovac, Nenad, Trkulja, Nenad, Milosavljević, Anja, Pfaf-Dolovac, Erika, "Influence of sugar beet cultivar resistance to Cercospora leaf spot threshold-reach and disease management" in Phytopathology, 104, no. 11 (2014):34-34.

TY  - JOUR
AU  - Bošković, Jelena
AU  - Zečević, Veselinka
AU  - Galonja-Coghill, Tamara
AU  - Matković, Mirela
AU  - Trkulja, Nenad
AU  - Vukašinović, Dragana
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/271
AB  - Biotechnology alongside the introduction of genetically modified (GM) crops is constantly providing new opportunities for increasing crop productivity and tackling problems in agriculture, such as diseases, pests and weeds, abiotic stress and nutritional limitations of stopple food crops. Crops possessing new traits enabling the use in pharmaceutical production are also being generated. As GM crops are being introduced into various locations with different ecosystems, agriculture, biodiversity and agriculture practice, a scientifically based understanding of the environmental effects of GM crops cultivation would assist decision markers worldwide in ensuring environmental safety and sustainability. The main important environmental assessment of GM crops deals with their putative invasiveness, vertical and/or horizontal gene flow, effects on biodiversity and the impact on other products. These investigations are all highly interdisciplinary and complex. This paper deals with some of the most important problems related to entering GM crops into the environment, such as plant protection, ecological effects of HRCs, gene flow, biodiversity, stress, ecological risks of Bt crops, effects on soil ecosystems etc. There is a clear need to further assess the severity, magnitude and scope of risks associated with the massive field deployment of transgenic crops. When assessing GMC inter-relation with the existing cultivars, an increased knowledge base underpinning the development of GMC will provide greater confidence in plant science while assessing the risks and benefits of releasing such crops.
AB  - Biotehnologija uz uvođenje genetski modifikovanih (GM) biljaka konstantno stvara nove mogućnosti povećanja biljne proizvodnje i rešavanja problema u poljoprivredi, kao što su bolesti, štetočine, korovi, abiotički stres i nutritivna ograničenja. Stvaraju se i biljke koje poseduju nove osobine, koje omogućavaju njihovo korištenje u farmaceutskoj proizvodnji. Budući da se GM biljke uvode na različite lokacije okarakterisane raznovrsnim ekosistemima, tipovima poljoprivrede, biodiverzitetom i agrikulturalnom praksom, neophodno je naucno razumevanje efekata uzgoja GM biljaka na životnu sredinu, koje ce obezbediti bezbednost i održivost životne sredine. Najvažnija istraživanja su ona usmerena na invazivnost GM biljaka, vertikalni i horizontalni prenos gena, uticaj na biološku raznovrsnost i na druge proizvode. Ova ispitivanja su veoma složena i multidisciplinarna. Ovaj rad razmatra neke od najvažnijih problema vezanih za uvođenje GM biljaka u životnu sredinu, kao što su zaštita biljaka, ekološki efekti horizontalnog prenosa gena, biodiverzitet, stres, efekti na zemljište itd. Postoji jasna potreba da se dublje istraži veličina i opseg rizika povezanih sa masivnom upotrebom transgenih biljaka. Pri procenjivanju međuodnosa GM biljaka I postojećih sorti, detaljnije poznavanje razvoja GM biljaka će omogućiti jasnije, pouzdanije i preciznije usmerene aktivnosti u biljnim naukama.
PB  - University of Kragujevac, Faculty of Agronomy, Cacak
T2  - Acta agriculturae Serbica
T1  - Assessing ecological aspects of biosafety of genetically modified crops to environment
T1  - Ocenjivanje ekoloških aspekata biološke sigurnosti genetski modifikovanih biljaka po životnu sredinu
EP  - 167
IS  - 36
SP  - 143
VL  - 18
ER  - 

@article{
author = "Bošković, Jelena and Zečević, Veselinka and Galonja-Coghill, Tamara and Matković, Mirela and Trkulja, Nenad and Vukašinović, Dragana",
year = "2013",
abstract = "Biotechnology alongside the introduction of genetically modified (GM) crops is constantly providing new opportunities for increasing crop productivity and tackling problems in agriculture, such as diseases, pests and weeds, abiotic stress and nutritional limitations of stopple food crops. Crops possessing new traits enabling the use in pharmaceutical production are also being generated. As GM crops are being introduced into various locations with different ecosystems, agriculture, biodiversity and agriculture practice, a scientifically based understanding of the environmental effects of GM crops cultivation would assist decision markers worldwide in ensuring environmental safety and sustainability. The main important environmental assessment of GM crops deals with their putative invasiveness, vertical and/or horizontal gene flow, effects on biodiversity and the impact on other products. These investigations are all highly interdisciplinary and complex. This paper deals with some of the most important problems related to entering GM crops into the environment, such as plant protection, ecological effects of HRCs, gene flow, biodiversity, stress, ecological risks of Bt crops, effects on soil ecosystems etc. There is a clear need to further assess the severity, magnitude and scope of risks associated with the massive field deployment of transgenic crops. When assessing GMC inter-relation with the existing cultivars, an increased knowledge base underpinning the development of GMC will provide greater confidence in plant science while assessing the risks and benefits of releasing such crops., Biotehnologija uz uvođenje genetski modifikovanih (GM) biljaka konstantno stvara nove mogućnosti povećanja biljne proizvodnje i rešavanja problema u poljoprivredi, kao što su bolesti, štetočine, korovi, abiotički stres i nutritivna ograničenja. Stvaraju se i biljke koje poseduju nove osobine, koje omogućavaju njihovo korištenje u farmaceutskoj proizvodnji. Budući da se GM biljke uvode na različite lokacije okarakterisane raznovrsnim ekosistemima, tipovima poljoprivrede, biodiverzitetom i agrikulturalnom praksom, neophodno je naucno razumevanje efekata uzgoja GM biljaka na životnu sredinu, koje ce obezbediti bezbednost i održivost životne sredine. Najvažnija istraživanja su ona usmerena na invazivnost GM biljaka, vertikalni i horizontalni prenos gena, uticaj na biološku raznovrsnost i na druge proizvode. Ova ispitivanja su veoma složena i multidisciplinarna. Ovaj rad razmatra neke od najvažnijih problema vezanih za uvođenje GM biljaka u životnu sredinu, kao što su zaštita biljaka, ekološki efekti horizontalnog prenosa gena, biodiverzitet, stres, efekti na zemljište itd. Postoji jasna potreba da se dublje istraži veličina i opseg rizika povezanih sa masivnom upotrebom transgenih biljaka. Pri procenjivanju međuodnosa GM biljaka I postojećih sorti, detaljnije poznavanje razvoja GM biljaka će omogućiti jasnije, pouzdanije i preciznije usmerene aktivnosti u biljnim naukama.",
publisher = "University of Kragujevac, Faculty of Agronomy, Cacak",
journal = "Acta agriculturae Serbica",
title = "Assessing ecological aspects of biosafety of genetically modified crops to environment, Ocenjivanje ekoloških aspekata biološke sigurnosti genetski modifikovanih biljaka po životnu sredinu",
pages = "167-143",
number = "36",
volume = "18"
}

Bošković, J., Zečević, V., Galonja-Coghill, T., Matković, M., Trkulja, N.,& Vukašinović, D.. (2013). Assessing ecological aspects of biosafety of genetically modified crops to environment. in Acta agriculturae Serbica
University of Kragujevac, Faculty of Agronomy, Cacak., 18(36), 143-167.

Bošković J, Zečević V, Galonja-Coghill T, Matković M, Trkulja N, Vukašinović D. Assessing ecological aspects of biosafety of genetically modified crops to environment. in Acta agriculturae Serbica. 2013;18(36):143-167..

Bošković, Jelena, Zečević, Veselinka, Galonja-Coghill, Tamara, Matković, Mirela, Trkulja, Nenad, Vukašinović, Dragana, "Assessing ecological aspects of biosafety of genetically modified crops to environment" in Acta agriculturae Serbica, 18, no. 36 (2013):143-167.

TY  - JOUR
AU  - Štrbanović, Ratibor
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Poštić, Dobrivoj
AU  - Marković, Jordan
AU  - Trkulja, Nenad
AU  - Dolovac, Nenad
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/256
AB  - In this study we investigated the presence of plant pathogenic genera of fungi on seeds of three different cultivars of alfalfa (K-22, NS-Banat and OS-88) with three different lots (locality) of each cultivars. When tested cultivars were identified following genera of fungi: Alternaria spp ., Fusarium spp., Penicillium spp., Mucor spp. and sterile mycelium. The presence of the identified genera of fungi ranged from 0% to 2%. Genus Alternaria spp. lowest average value was in alfalfa cultivars K-22 (0.42%), while the highest average value of a cultivar OS-88 (0.58 %), and also at the same cultivar was noted by the presence of one (1%) at the locality of Osijek I. In all the cultivars of alfalfa, a high coefficient of variation, indicating a high variability within the three cultivars for this trait. The highest average attendance of the genus Fusarium spp. was observed in cultivar OS-88 (0.75%), and in the same cultivar is the largest presence (1.25%) were recorded at the locality of Osijek II. For the studied alfalfa cultivars were examined correlations between parameters of seed quality and the presence of pathogens on seeds of different cultivars of alfalfa and lots. Strong positive correlations were observed between germination energy and total germi­nation (r=0.891***), and between mass of 1000 seeds and the presence of Fusarium spp. (r=0.797**). The results of these tests indicate satisfactory state of health of all cultivars and alfalfa seed lots and in particular in relation to the economically important phytopathogenic fungi of the genus Fusarium spp.
AB  - U ovom radu je ispitivano prisustvo fitopatogenih rodova gljiva na semenu tri različite sorte lucerke (K-22, NS-Banat i OS-88) sa po tri različite partije (lokaliteta) od svake sorte. Kod ispitivanih sorti identifikovani su sledeći rodovi gljiva: Alternaria spp., Fusarium spp., Penicillium spp., Mucor spp. i sterilna micelija. Prisustvo identifikovanih rodova gljiva kretalo se u rasponu od 0% do 2%. Rod Alternaria spp. najmanju prosečnu vrednost imao je kod sorte lucerke K-22 (0.42%), dok je najveću prosečnu vrednost imao kod sorte OS-88 (0.58%), a takođe kod iste sorte konstatovano je najveće prisustvo od (1%) na lokalitetu Osijek I. Kod svih ispitivanih sorata lucerke utvrđen je visok koeficijent varijacije, što ukazuje na visoku varijabilnost unutar samih sorata za ovo svojstvo. Najveće prosečno prisustvo gljiva roda Fusarium spp. zabeleženo je kod sorte OS-88 (0.75%), a kod iste sorte je najveće prisustvo (1.25%) zabeleženo na lokalitetu Osijek II. Na proučavanim sortama lucerke ispitani su korelacioni odnosi između parametara kvaliteta semena i prisustva patogena na semenu različitih sorata i partija lucerke. Jake pozitivne korelacije zabeležene su između energije klijanja i ukupne klijavosti (r=0,891***), kao i između mase 1000 semena i prisustva gljiva iz roda Fusarium spp. (r=0,797**). Rezultati ovih ispitivanja ukazuju na zadovoljavajuće zdravstveno stanje svih sorata i partija semena lucerke a posebno u odnosu na ekonomski značajne fitopatogene gljive iz roda Fusarium spp.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke
T1  - Health testing different genotypes alfalfa seeds
EP  - 217
IS  - 4
SP  - 212
VL  - 64
ER  - 

@article{
author = "Štrbanović, Ratibor and Gavrilović, Veljko and Stanisavljević, Rade and Poštić, Dobrivoj and Marković, Jordan and Trkulja, Nenad and Dolovac, Nenad",
year = "2013",
abstract = "In this study we investigated the presence of plant pathogenic genera of fungi on seeds of three different cultivars of alfalfa (K-22, NS-Banat and OS-88) with three different lots (locality) of each cultivars. When tested cultivars were identified following genera of fungi: Alternaria spp ., Fusarium spp., Penicillium spp., Mucor spp. and sterile mycelium. The presence of the identified genera of fungi ranged from 0% to 2%. Genus Alternaria spp. lowest average value was in alfalfa cultivars K-22 (0.42%), while the highest average value of a cultivar OS-88 (0.58 %), and also at the same cultivar was noted by the presence of one (1%) at the locality of Osijek I. In all the cultivars of alfalfa, a high coefficient of variation, indicating a high variability within the three cultivars for this trait. The highest average attendance of the genus Fusarium spp. was observed in cultivar OS-88 (0.75%), and in the same cultivar is the largest presence (1.25%) were recorded at the locality of Osijek II. For the studied alfalfa cultivars were examined correlations between parameters of seed quality and the presence of pathogens on seeds of different cultivars of alfalfa and lots. Strong positive correlations were observed between germination energy and total germi­nation (r=0.891***), and between mass of 1000 seeds and the presence of Fusarium spp. (r=0.797**). The results of these tests indicate satisfactory state of health of all cultivars and alfalfa seed lots and in particular in relation to the economically important phytopathogenic fungi of the genus Fusarium spp., U ovom radu je ispitivano prisustvo fitopatogenih rodova gljiva na semenu tri različite sorte lucerke (K-22, NS-Banat i OS-88) sa po tri različite partije (lokaliteta) od svake sorte. Kod ispitivanih sorti identifikovani su sledeći rodovi gljiva: Alternaria spp., Fusarium spp., Penicillium spp., Mucor spp. i sterilna micelija. Prisustvo identifikovanih rodova gljiva kretalo se u rasponu od 0% do 2%. Rod Alternaria spp. najmanju prosečnu vrednost imao je kod sorte lucerke K-22 (0.42%), dok je najveću prosečnu vrednost imao kod sorte OS-88 (0.58%), a takođe kod iste sorte konstatovano je najveće prisustvo od (1%) na lokalitetu Osijek I. Kod svih ispitivanih sorata lucerke utvrđen je visok koeficijent varijacije, što ukazuje na visoku varijabilnost unutar samih sorata za ovo svojstvo. Najveće prosečno prisustvo gljiva roda Fusarium spp. zabeleženo je kod sorte OS-88 (0.75%), a kod iste sorte je najveće prisustvo (1.25%) zabeleženo na lokalitetu Osijek II. Na proučavanim sortama lucerke ispitani su korelacioni odnosi između parametara kvaliteta semena i prisustva patogena na semenu različitih sorata i partija lucerke. Jake pozitivne korelacije zabeležene su između energije klijanja i ukupne klijavosti (r=0,891***), kao i između mase 1000 semena i prisustva gljiva iz roda Fusarium spp. (r=0,797**). Rezultati ovih ispitivanja ukazuju na zadovoljavajuće zdravstveno stanje svih sorata i partija semena lucerke a posebno u odnosu na ekonomski značajne fitopatogene gljive iz roda Fusarium spp.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke, Health testing different genotypes alfalfa seeds",
pages = "217-212",
number = "4",
volume = "64"
}

Štrbanović, R., Gavrilović, V., Stanisavljević, R., Poštić, D., Marković, J., Trkulja, N.,& Dolovac, N.. (2013). Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 64(4), 212-217.

Štrbanović R, Gavrilović V, Stanisavljević R, Poštić D, Marković J, Trkulja N, Dolovac N. Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke. in Zaštita bilja. 2013;64(4):212-217..

Štrbanović, Ratibor, Gavrilović, Veljko, Stanisavljević, Rade, Poštić, Dobrivoj, Marković, Jordan, Trkulja, Nenad, Dolovac, Nenad, "Ispitivanje zdravstvenog stanja različitih genotipova semena lucerke" in Zaštita bilja, 64, no. 4 (2013):212-217.

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Ivanović, Žarko
AU  - Pfaf-Dolovac, Erika
AU  - Dolovac, Nenad
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/272
AB  - A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene
EP  - 902
IS  - 4
SP  - 889
VL  - 135
DO  - 10.1007/s10658-012-0135-x
ER  - 

@article{
author = "Trkulja, Nenad and Ivanović, Žarko and Pfaf-Dolovac, Erika and Dolovac, Nenad and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2013",
abstract = "A survey to detect and characterise benzimidazole resistance within populations of Cercospora beticola in Serbia was performed. From 52 field isolates collected from sugar beet and beet root, only eight were found to be benzimidazole-sensitive based on the inhibition of mycelial growth by discriminatory concentrations of carbendazim and thiophanate-methyl. Sensitivity tests revealed the presence of three resistant phenotypes among the tested isolates: high-resistance (HR), low-resistance (LR) and moderate-resistance (MR). The benzimidazole resistant isolates were characterised based on the DNA sequence of the beta-tubulin gene and temperature sensitivity. The HR isolates showed no temperature sensitivity regardless of carbendazim concentration, whereas the LR and MR isolates were sensitive at lower temperatures. Analysis of the beta-tubulin gene sequence revealed two amino acid replacements in the benzimidazole-resistant isolates of C. beticola. One was a glutamic acid to alanine change at position 198 (codon GAG to GCG) that was identified in HR isolates; this mutation has previously been reported to be associated with the development of benzimidazole resistance in C. beticola. The second replacement was a novel point mutation of phenylalanine (TTC) to tyrosine (TAC) at position 167, identified in low and moderate benzimidazole-resistant isolates, sharing a single LR/MR beta-tubulin genotype. A diagnostic PCR-RFLP assay utilising a BsaI restriction site present in the benzimidazole sensitive and LR/MR genotypes but absent in the HR genotype was developed for the routine detection of high resistance. A mutation-specific PCR assay was developed for the diagnosis of LR/MR genotype based on a mutation from T to A at codon 167, which is unique to this genotype.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene",
pages = "902-889",
number = "4",
volume = "135",
doi = "10.1007/s10658-012-0135-x"
}

Trkulja, N., Ivanović, Ž., Pfaf-Dolovac, E., Dolovac, N., Mitrović, M., Toševski, I.,& Jović, J.. (2013). Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology
Springer, Dordrecht., 135(4), 889-902.
https://doi.org/10.1007/s10658-012-0135-x

Trkulja N, Ivanović Ž, Pfaf-Dolovac E, Dolovac N, Mitrović M, Toševski I, Jović J. Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene. in European Journal of Plant Pathology. 2013;135(4):889-902.
doi:10.1007/s10658-012-0135-x .

Trkulja, Nenad, Ivanović, Žarko, Pfaf-Dolovac, Erika, Dolovac, Nenad, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Characterisation of benzimidazole resistance of Cercospora beticola in Serbia using PCR-based detection of resistance-associated mutations of the beta-tubulin gene" in European Journal of Plant Pathology, 135, no. 4 (2013):889-902,
https://doi.org/10.1007/s10658-012-0135-x . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Ignjatov, Maja
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/283
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.
EP  - 1504
IS  - 11
SP  - 1504
VL  - 97
DO  - 10.1094/PDIS-03-13-0267-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Živković, Svetlana and Trkulja, Nenad and Ignjatov, Maja",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.",
pages = "1504-1504",
number = "11",
volume = "97",
doi = "10.1094/PDIS-03-13-0267-PDN"
}

Popović, T., Ivanović, Ž., Živković, S., Trkulja, N.,& Ignjatov, M.. (2013). First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(11), 1504-1504.
https://doi.org/10.1094/PDIS-03-13-0267-PDN

Popović T, Ivanović Ž, Živković S, Trkulja N, Ignjatov M. First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia.. in Plant Disease. 2013;97(11):1504-1504.
doi:10.1094/PDIS-03-13-0267-PDN .

Popović, Tatjana, Ivanović, Žarko, Živković, Svetlana, Trkulja, Nenad, Ignjatov, Maja, "First Report of Brenneria nigrifluens as the Causal Agent of Shallow-Bark Canker on Walnut Trees (Juglans regia) in Serbia." in Plant Disease, 97, no. 11 (2013):1504-1504,
https://doi.org/10.1094/PDIS-03-13-0267-PDN . .