TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Pfaf-Dolovac, Erika
AU  - Aleksić, Goran
AU  - Vučurović, Ana
AU  - Starović, Mira
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/560
AB  - It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
PB  - Springer, Dordrecht
T2  - Potato Research
T1  - The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops
EP  - 46
IS  - 1
SP  - 31
VL  - 62
DO  - 10.1007/s11540-018-9395-y
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Pfaf-Dolovac, Erika and Aleksić, Goran and Vučurović, Ana and Starović, Mira",
year = "2019",
abstract = "It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.",
publisher = "Springer, Dordrecht",
journal = "Potato Research",
title = "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops",
pages = "46-31",
number = "1",
volume = "62",
doi = "10.1007/s11540-018-9395-y"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Pfaf-Dolovac, E., Aleksić, G., Vučurović, A.,& Starović, M.. (2019). The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research
Springer, Dordrecht., 62(1), 31-46.
https://doi.org/10.1007/s11540-018-9395-y

Ristić D, Vučurović I, Kuzmanović S, Pfaf-Dolovac E, Aleksić G, Vučurović A, Starović M. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research. 2019;62(1):31-46.
doi:10.1007/s11540-018-9395-y .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Pfaf-Dolovac, Erika, Aleksić, Goran, Vučurović, Ana, Starović, Mira, "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops" in Potato Research, 62, no. 1 (2019):31-46,
https://doi.org/10.1007/s11540-018-9395-y . .

TY  - JOUR
AU  - Suvajdzić, Branko
AU  - Vasilev, Dragan
AU  - Karabasil, Nedeljko
AU  - Vučurović, Ivan
AU  - Čobanović, Nikola
AU  - Babić, Milijana
AU  - Katić, Vera
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/486
AB  - Algae from the genus Prototheca are the only plant-like microorganisms which can cause inflammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identification of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specific PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfii, of which Prototheca zopfii genotype 3 was reclassified in a new species: Prototheca blaschkeae. P. zopfii genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to significant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis, as well as to determine the influence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfii genotype 2 by a genotype-specific PCR. This study has provided the first molecular identification of Prototheca zopfii genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 +/- 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfii genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfii genotype 2 is the main causative agent of Prototheca mastitis which leads to a significant increase in the somatic cell count in the milk.
PB  - Univ Zagreb Vet Faculty, Zagreb
T2  - Veterinarski Arhiv
T1  - Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count
EP  - 258
IS  - 3
SP  - 249
VL  - 87
DO  - 10.24099/vet.arhiv.151219
ER  - 

@article{
author = "Suvajdzić, Branko and Vasilev, Dragan and Karabasil, Nedeljko and Vučurović, Ivan and Čobanović, Nikola and Babić, Milijana and Katić, Vera",
year = "2017",
abstract = "Algae from the genus Prototheca are the only plant-like microorganisms which can cause inflammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identification of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specific PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfii, of which Prototheca zopfii genotype 3 was reclassified in a new species: Prototheca blaschkeae. P. zopfii genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to significant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis, as well as to determine the influence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfii genotype 2 by a genotype-specific PCR. This study has provided the first molecular identification of Prototheca zopfii genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 +/- 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfii genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfii genotype 2 is the main causative agent of Prototheca mastitis which leads to a significant increase in the somatic cell count in the milk.",
publisher = "Univ Zagreb Vet Faculty, Zagreb",
journal = "Veterinarski Arhiv",
title = "Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count",
pages = "258-249",
number = "3",
volume = "87",
doi = "10.24099/vet.arhiv.151219"
}

Suvajdzić, B., Vasilev, D., Karabasil, N., Vučurović, I., Čobanović, N., Babić, M.,& Katić, V.. (2017). Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count. in Veterinarski Arhiv
Univ Zagreb Vet Faculty, Zagreb., 87(3), 249-258.
https://doi.org/10.24099/vet.arhiv.151219

Suvajdzić B, Vasilev D, Karabasil N, Vučurović I, Čobanović N, Babić M, Katić V. Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count. in Veterinarski Arhiv. 2017;87(3):249-258.
doi:10.24099/vet.arhiv.151219 .

Suvajdzić, Branko, Vasilev, Dragan, Karabasil, Nedeljko, Vučurović, Ivan, Čobanović, Nikola, Babić, Milijana, Katić, Vera, "Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count" in Veterinarski Arhiv, 87, no. 3 (2017):249-258,
https://doi.org/10.24099/vet.arhiv.151219 . .

TY  - JOUR
AU  - Vučurović, Ivan
AU  - Nikolić, Dušan
AU  - Radović, Nikola
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/492
AB  - Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
AB  - Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Incidence and distribution of leek yellow stripe virus in allium crops in Serbia
EP  - 155
IS  - 3-4
SP  - 145
VL  - 32
DO  - 10.2298/PIF1704145V
ER  - 

@article{
author = "Vučurović, Ivan and Nikolić, Dušan and Radović, Nikola and Vučurović, Ana and Ristić, Danijela and Krstić, Branka and Stanković, Ivana",
year = "2017",
abstract = "Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E., Virus žute prugavosti praziluka (Leek yellow stripe virus, LYSV) je jedan od najčešćih i najznačajnijih virusa na praziluku i belom luku. U Srbiji je virus detektovan na obe kulture, i praziluku i belom luku, a često se javlja u visokim procentima. Tokom dve uzastopne godine (2013. i 2014.) pregledano je 11 lokaliteta gajenja praziluka, belog i crnog luka i sakupljena su 92 uzorka koja su DAS-ELISA metodom testirana na prisustvo LYSV. Prisustvo LYSV je dokazano u 31,5% testiranih uzoraka. Tokom 2012., prisustvo LYSV dokazano je samo u praziluku, u 55,6% testiranih uzoraka. Tokom 2013., LYSV je dokazan u 85% uzoraka praziluka i 58,3% uzoraka belog luka. Ukupno, prisustvo LYSV je dokazano u 56,4% uzoraka praziluka i 17,1% uzoraka belog luka. Prisustvo LYSV u testiranim uzorcima potvrđeno je primenom RT-PCR metode i specifičnih prajmera za LYSV koji umnožavaju fragment od 1020 bp koji obuhvata gen za proteinski omotač i deo gena za nuklearne inkluzije B. Molekularna identifikacija LYSV obavljena je sekvenciranjem dva odabrana izolata iz belog luka 181-13 (MG242625) i praziluka 298-13 (MG242624) i poređenjem dobijenih sekvenci sa sekvencama LYSV iz GenBank baze podataka. Filogenetske analize 55 sekvenci izolata LYSV iz različitih delova sveta ukazale su na delimičnu korelaciju između biljke domaćina i geografskog porekla izolata, formiranjem pet odvojenih grupa izolata u stablu. Dva izolata iz Srbije grupisala su se u udaljene grupe. Izolat iz Srbije iz praziluka 298-13 grupisao se u grupu B, dok se izolat iz belog luka 181-13 grupisao u grupu E.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia",
pages = "155-145",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704145V"
}

Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B.,& Stanković, I.. (2017). Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 145-155.
https://doi.org/10.2298/PIF1704145V

Vučurović I, Nikolić D, Radović N, Vučurović A, Ristić D, Krstić B, Stanković I. Incidence and distribution of leek yellow stripe virus in allium crops in Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):145-155.
doi:10.2298/PIF1704145V .

Vučurović, Ivan, Nikolić, Dušan, Radović, Nikola, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, Stanković, Ivana, "Incidence and distribution of leek yellow stripe virus in allium crops in Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):145-155,
https://doi.org/10.2298/PIF1704145V . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Milosević, Drago
AU  - Gašić, Katarina
AU  - Dolovac, Nenad
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/449
AB  - The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia
EP  - 496
IS  - 2
SP  - 487
VL  - 48
DO  - 10.2298/GENSR1602487R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Milosević, Drago and Gašić, Katarina and Dolovac, Nenad and Starović, Mira",
year = "2016",
abstract = "The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate's complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia",
pages = "496-487",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602487R"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Milosević, D., Gašić, K., Dolovac, N.,& Starović, M.. (2016). Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 48(2), 487-496.
https://doi.org/10.2298/GENSR1602487R

Ristić D, Vučurović I, Kuzmanović S, Milosević D, Gašić K, Dolovac N, Starović M. Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia. in Genetika-Belgrade. 2016;48(2):487-496.
doi:10.2298/GENSR1602487R .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Milosević, Drago, Gašić, Katarina, Dolovac, Nenad, Starović, Mira, "Molecular characterization of potato virus Y inducing potato tuber necrotic ringspot disease in Serbia" in Genetika-Belgrade, 48, no. 2 (2016):487-496,
https://doi.org/10.2298/GENSR1602487R . .

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
AU  - Stojanović, Sasa
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/450
AB  - Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.
PB  - Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia
EP  - 417
IS  - 2
SP  - 411
VL  - 44
DO  - 10.15835/nbha44210488
ER  - 

@article{
author = "Pavlović, Snežana and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš and Stojanović, Sasa and Kuzmanović, Slobodan and Starović, Mira",
year = "2016",
abstract = "Anise (Pimpinella anzsum L.) is an important medicinal spice plant that belongs to the family Apiaceae. Anise seeds are rich in essential oils and this is a reason why anise production in Serbia has increased over the last decade. During a routine health inspection on anise seeds collected from three localities in the province of Vojvodina (Mogorin, Veliki Radinci and Ostojkevo) during 2012 and 2013, it was found out that Fusarium spp. were a commonly observed fungi. The presence of Fusarium fungi on the seed samples ranged from 3.75-13.75%. The aim of this study was to isolate and identify the strains of Fusarium species present on anise seed samples as it is necessary that commercially used anise seeds are completely free of Fusarium. Based on morphological, microscopic characteristics and a molecular identification by sequencing of TEF gene, the presence of the following species was confirmed on the anise seeds: F. trzeinctum, F. proliferatum, F. equiseti, F. oxysporum, F. sperotrzchozcles, F. incarnatum and F. verttallzoteles. According to our knowledge and research, this is the first report of F. trianctum and F. sporotrichoi des as pathogens on anise seeds in the world. All seven isolates of Fusarium species are pathogenic to the anise seedlings, while the most virulent species were F. oxysporum, F. tricznetum and F. incarnation.",
publisher = "Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia",
pages = "417-411",
number = "2",
volume = "44",
doi = "10.15835/nbha44210488"
}

Pavlović, S., Ristić, D., Vučurović, I., Stevanović, M., Stojanović, S., Kuzmanović, S.,& Starović, M.. (2016). Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Univ Agr Sci & Veterinary Med Cluj-Napoca, Cluj-Napoca., 44(2), 411-417.
https://doi.org/10.15835/nbha44210488

Pavlović S, Ristić D, Vučurović I, Stevanović M, Stojanović S, Kuzmanović S, Starović M. Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2016;44(2):411-417.
doi:10.15835/nbha44210488 .

Pavlović, Snežana, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, Stojanović, Sasa, Kuzmanović, Slobodan, Starović, Mira, "Morphology, Pathogenicity and Molecular Identification of Fusarium spp. Associated with Anise Seeds in Serbia" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44, no. 2 (2016):411-417,
https://doi.org/10.15835/nbha44210488 . .