TY  - JOUR
AU  - Gosik, Rafal
AU  - Caldara, Roberto
AU  - Toševski, Ivo
AU  - Skuhrovec, Jiří
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1227
AB  - The mature larvae of the following fourteen Rhinusa species are described and illustrated:
Rhinusa antirrhini (Paykull, 1800), R. asellus (Gravenhorst, 1807), R. collina (Gyllenhal,
1813), R. eversmanni (Rosenschoeld, 1838), R. florum (Rubsaamen, 1895), R. herbarum
(H. Brisout de Barneville, 1862), R. incana (Kirsch, 1881), R. linariae (Panzer, 1796),
R. melas (Boheman, 1838), R. neta (Germar, 1821), R. pilosa (Gyllenhal, 1838), R. rara
Toševski & Caldara, 2015, R. tetra (Fabricius, 1792), and R. vestita (Germar, 1821). The
pupae of thirteen of them (except R. incana) were also described. The comparison of
larval morphological characters and plant preferences provides evidence supporting
the existence of different species groups previously established according to a phylogenetic
analysis based on adult morphological characters. The following diagnostic
attributes distinguishing the genus Rhinusa are highlighted. For the larvae: (1) pronotal
shield indistinct; (2) thoracic prodorsal fold small or even vestigial; (3) abdominal postdorsal
folds (especially of segments III–VII) high or even in the form of conical protuberances;
(4) cuticle of abdominal segments densely covered with asperities; (5) cuticle
without dark spots or dark pigmentation; (6) head suboval, rarely round; (7) labrum usually
with 2 als; (8) des1 short or absent, rarely elongated; and (9) fs1-3 usually absent or
minute. For the pupae: (1) body stout; (2) head protuberances always present; (3) pronotal
protuberances (if present), separated at bases of the pronotum, always wider than
higher; (4) abdominal protuberance usually present, wide or round; (5) femora usually
with a single fes; and (6) urogomphi short or vestigial. Keys to the larvae and pupae
described here are provided. All the characters used for identification are illustrated by
photographs or drawings. Biological and distribution data, including new information,
are provided for all the species studied.
PB  - Pensoft Publishers
T2  - ZooKeys
T1  - Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels
EP  - 94
SP  - 1
VL  - 1195
DO  - 10.3897/zookeys.1195.112328
ER  - 

@article{
author = "Gosik, Rafal and Caldara, Roberto and Toševski, Ivo and Skuhrovec, Jiří",
year = "2024",
abstract = "The mature larvae of the following fourteen Rhinusa species are described and illustrated:
Rhinusa antirrhini (Paykull, 1800), R. asellus (Gravenhorst, 1807), R. collina (Gyllenhal,
1813), R. eversmanni (Rosenschoeld, 1838), R. florum (Rubsaamen, 1895), R. herbarum
(H. Brisout de Barneville, 1862), R. incana (Kirsch, 1881), R. linariae (Panzer, 1796),
R. melas (Boheman, 1838), R. neta (Germar, 1821), R. pilosa (Gyllenhal, 1838), R. rara
Toševski & Caldara, 2015, R. tetra (Fabricius, 1792), and R. vestita (Germar, 1821). The
pupae of thirteen of them (except R. incana) were also described. The comparison of
larval morphological characters and plant preferences provides evidence supporting
the existence of different species groups previously established according to a phylogenetic
analysis based on adult morphological characters. The following diagnostic
attributes distinguishing the genus Rhinusa are highlighted. For the larvae: (1) pronotal
shield indistinct; (2) thoracic prodorsal fold small or even vestigial; (3) abdominal postdorsal
folds (especially of segments III–VII) high or even in the form of conical protuberances;
(4) cuticle of abdominal segments densely covered with asperities; (5) cuticle
without dark spots or dark pigmentation; (6) head suboval, rarely round; (7) labrum usually
with 2 als; (8) des1 short or absent, rarely elongated; and (9) fs1-3 usually absent or
minute. For the pupae: (1) body stout; (2) head protuberances always present; (3) pronotal
protuberances (if present), separated at bases of the pronotum, always wider than
higher; (4) abdominal protuberance usually present, wide or round; (5) femora usually
with a single fes; and (6) urogomphi short or vestigial. Keys to the larvae and pupae
described here are provided. All the characters used for identification are illustrated by
photographs or drawings. Biological and distribution data, including new information,
are provided for all the species studied.",
publisher = "Pensoft Publishers",
journal = "ZooKeys",
title = "Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels",
pages = "94-1",
volume = "1195",
doi = "10.3897/zookeys.1195.112328"
}

Gosik, R., Caldara, R., Toševski, I.,& Skuhrovec, J.. (2024). Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels. in ZooKeys
Pensoft Publishers., 1195, 1-94.
https://doi.org/10.3897/zookeys.1195.112328

Gosik R, Caldara R, Toševski I, Skuhrovec J. Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels. in ZooKeys. 2024;1195:1-94.
doi:10.3897/zookeys.1195.112328 .

Gosik, Rafal, Caldara, Roberto, Toševski, Ivo, Skuhrovec, Jiří, "Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels" in ZooKeys, 1195 (2024):1-94,
https://doi.org/10.3897/zookeys.1195.112328 . .

TY  - CONF
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1215
AB  - “Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.
C3  - European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy
T1  - Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia
EP  - 54
SP  - 53
ER  - 

@conference{
author = "Jović, Jelena and Cvrković, Tatjana and Krstić, Oliver and Mitrović, Milana and Jakovljević, Miljana and Marinković, Slavica and Toševski, Ivo",
year = "2024",
abstract = "“Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.",
journal = "European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy",
title = "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia",
pages = "54-53"
}

Jović, J., Cvrković, T., Krstić, O., Mitrović, M., Jakovljević, M., Marinković, S.,& Toševski, I.. (2024). Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy, 53-54.

Jović J, Cvrković T, Krstić O, Mitrović M, Jakovljević M, Marinković S, Toševski I. Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy. 2024;:53-54..

Jović, Jelena, Cvrković, Tatjana, Krstić, Oliver, Mitrović, Milana, Jakovljević, Miljana, Marinković, Slavica, Toševski, Ivo, "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia" in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy (2024):53-54.

TY  - CONF
AU  - Delač, Magdalena
AU  - Polak, Bruno
AU  - Drčelić, Marina
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Šeruga Musić, Martina
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1216
AB  - Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.
C3  - Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023
T1  - Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich
EP  - 185
SP  - 184
ER  - 

@conference{
author = "Delač, Magdalena and Polak, Bruno and Drčelić, Marina and Jović, Jelena and Krstić, Oliver and Toševski, Ivo and Cvrković, Tatjana and Jakovljević, Miljana and Šeruga Musić, Martina",
year = "2023",
abstract = "Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.",
journal = "Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023",
title = "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich",
pages = "185-184"
}

Delač, M., Polak, B., Drčelić, M., Jović, J., Krstić, O., Toševski, I., Cvrković, T., Jakovljević, M.,& Šeruga Musić, M.. (2023). Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023, 184-185.

Delač M, Polak B, Drčelić M, Jović J, Krstić O, Toševski I, Cvrković T, Jakovljević M, Šeruga Musić M. Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023. 2023;:184-185..

Delač, Magdalena, Polak, Bruno, Drčelić, Marina, Jović, Jelena, Krstić, Oliver, Toševski, Ivo, Cvrković, Tatjana, Jakovljević, Miljana, Šeruga Musić, Martina, "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich" in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023 (2023):184-185.

TY  - JOUR
AU  - Toševski, Ivo
AU  - Caldara, Roberto
AU  - Tosevski, Ivo
AU  - Mendel, Howard
AU  - Germann, Cristoph
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/665
AB  - During morphological and molecular studies of species of Rhamphus from southern and western Europe, the authors realized that some specimens currently identified as R. pulicarius (Herbst, 1795) and R. oxyacanthae (Marsham, 1802), two widely distributed species in Europe, belonged to different closely related taxa. Therefore, it was necessary to examine type specimens of these and other species considered synonymous or confused with them. It was possible to designate lectotypes for Rhamphus subaeneus Illiger, 1808 and Rhamphus kiesenwetteri Tournier, 1873. However, it was necessary to designate neotypes for: Curculio pulicarius Herbst, 1795, Curculio oxyacanthae Marsham, 1802 and Rhamphus flavicornis [Clairville], 1798. These three neotypes were sequenced for mtCOI and the sequences are now accessible online. Moreover, in accordance with Article 23.9 and the Principle of Priority of the Code, because of confirmed synonymy between R. oxyacanthae and R. flavicornis, the former name is officially proposed as nomen protectum and the latter as nomen oblitum. Detailed information regarding historical entomological collections in the Museum fr Naturkunde der Humboldt-Universitt of Berlin and the Clairville collection in the Naturhistorisches Museum of Basel, relating to our search for relevant type specimens, is provided.
PB  - Magnolia Press
T2  - Zootaxa
T1  - In search of some type-specimens of Rhamphus [Clairville], 1798 (Coleoptera: Curculionidae)
EP  - 380
IS  - 4
SP  - 371
VL  - 5169
DO  - 10.11646/zootaxa.5169.4.6
ER  - 

@article{
author = "Toševski, Ivo and Caldara, Roberto and Tosevski, Ivo and Mendel, Howard and Germann, Cristoph",
year = "2022",
abstract = "During morphological and molecular studies of species of Rhamphus from southern and western Europe, the authors realized that some specimens currently identified as R. pulicarius (Herbst, 1795) and R. oxyacanthae (Marsham, 1802), two widely distributed species in Europe, belonged to different closely related taxa. Therefore, it was necessary to examine type specimens of these and other species considered synonymous or confused with them. It was possible to designate lectotypes for Rhamphus subaeneus Illiger, 1808 and Rhamphus kiesenwetteri Tournier, 1873. However, it was necessary to designate neotypes for: Curculio pulicarius Herbst, 1795, Curculio oxyacanthae Marsham, 1802 and Rhamphus flavicornis [Clairville], 1798. These three neotypes were sequenced for mtCOI and the sequences are now accessible online. Moreover, in accordance with Article 23.9 and the Principle of Priority of the Code, because of confirmed synonymy between R. oxyacanthae and R. flavicornis, the former name is officially proposed as nomen protectum and the latter as nomen oblitum. Detailed information regarding historical entomological collections in the Museum fr Naturkunde der Humboldt-Universitt of Berlin and the Clairville collection in the Naturhistorisches Museum of Basel, relating to our search for relevant type specimens, is provided.",
publisher = "Magnolia Press",
journal = "Zootaxa",
title = "In search of some type-specimens of Rhamphus [Clairville], 1798 (Coleoptera: Curculionidae)",
pages = "380-371",
number = "4",
volume = "5169",
doi = "10.11646/zootaxa.5169.4.6"
}

Toševski, I., Caldara, R., Tosevski, I., Mendel, H.,& Germann, C.. (2022). In search of some type-specimens of Rhamphus [Clairville], 1798 (Coleoptera: Curculionidae). in Zootaxa
Magnolia Press., 5169(4), 371-380.
https://doi.org/10.11646/zootaxa.5169.4.6

Toševski I, Caldara R, Tosevski I, Mendel H, Germann C. In search of some type-specimens of Rhamphus [Clairville], 1798 (Coleoptera: Curculionidae). in Zootaxa. 2022;5169(4):371-380.
doi:10.11646/zootaxa.5169.4.6 .

Toševski, Ivo, Caldara, Roberto, Tosevski, Ivo, Mendel, Howard, Germann, Cristoph, "In search of some type-specimens of Rhamphus [Clairville], 1798 (Coleoptera: Curculionidae)" in Zootaxa, 5169, no. 4 (2022):371-380,
https://doi.org/10.11646/zootaxa.5169.4.6 . .

TY  - JOUR
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/588
AB  - Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.
PB  - Springer Nature
T2  - European Journal of Plant Pathology
T1  - Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential
EP  - 221
SP  - 201
VL  - 156
DO  - 10.1007/s10658-019-01878-w
ER  - 

@article{
author = "Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Mitrović, Milana and Marinković, Slavica and Toševski, Ivo and Cvrković, Tatjana",
year = "2020",
abstract = "Euscelis incisus is polyphagous leafhopper that is widely distributed and abundant in diverse agroecosystems. Two generations per year allows this species to come into contact with a plethora of different phytoplasmas. We first investigated the interactions of E. incisus with phytoplasmas in different ecosystems as a bioindicator of phytoplasma diversity. Among the 510 analyzed E. incisus specimens collected from eight localities in Serbia, 53 (10%) tested positive for diverse ‘Ca. Phytoplasma’ taxa. Restriction fragment length polymorphism (RFLP) analysis and sequence comparison based on the F2nR2 fragment of the 16S rRNA gene revealed the presence of six 16S phytoplasma ribosomal groups and eight subgroups in E. incisus (16SrI-R, 16SrI-F, 16SrII-E, 16SrIII-B, 16SrIX-C, 16SrIX-E, 16SrXI-G and 16SrXII-A). The most prevalent was the 16SrXII-A phytoplasma group, represented by two multilocus stolbur phytoplasma genotypes: STOLg and Rqg31g. While the 16SrXII-A phytoplasmas were identical to the previously recorded isolates from Serbia, the 16SrI-F and 16SrI-R subgroups closely related to the previously documented 16SrI-B and 16SrI-C subgroups were recorded for the first time in Serbia. In addition, records of the 16SrIX and 16SrXI groups represent the first findings of these phytoplasma groups in Serbia. Transmission trials of ‘Ca. P. solani’ were performed with naturally infected E. incisus adults. The leafhoppers successfully transmitted stolbur phytoplasma to exposed Catharanthus roseus plants, indicating their role as a natural vector.",
publisher = "Springer Nature",
journal = "European Journal of Plant Pathology",
title = "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential",
pages = "221-201",
volume = "156",
doi = "10.1007/s10658-019-01878-w"
}

Jakovljević, M., Jović, J., Krstić, O., Mitrović, M., Marinković, S., Toševski, I.,& Cvrković, T.. (2020). Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology
Springer Nature., 156, 201-221.
https://doi.org/10.1007/s10658-019-01878-w

Jakovljević M, Jović J, Krstić O, Mitrović M, Marinković S, Toševski I, Cvrković T. Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential. in European Journal of Plant Pathology. 2020;156:201-221.
doi:10.1007/s10658-019-01878-w .

Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Mitrović, Milana, Marinković, Slavica, Toševski, Ivo, Cvrković, Tatjana, "Diversity of phytoplasmas identified in the polyphagous leafhopper Euscelis incisus (Cicadellidae, Deltocephalinae) in Serbia: pathogen inventory, epidemiological significance and vectoring potential" in European Journal of Plant Pathology, 156 (2020):201-221,
https://doi.org/10.1007/s10658-019-01878-w . .

TY  - JOUR
AU  - Sedlarević-Zorić, Ana
AU  - Morina, Filis
AU  - Toševski, Ivo
AU  - Tosti, Tomislav
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/569
AB  - Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.
PB  - Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales
T2  - Plant Physiology and Biochemistry
T1  - Resource allocation in response to herbivory and gall formation in Linaria vulgaris
EP  - 232
SP  - 224
VL  - 135
DO  - 10.1016/j.plaphy.2018.11.032
ER  - 

@article{
author = "Sedlarević-Zorić, Ana and Morina, Filis and Toševski, Ivo and Tosti, Tomislav and Jović, Jelena and Krstić, Oliver and Veljović-Jovanović, Sonja",
year = "2019",
abstract = "Trehalose and its precursor, trehalose 6-phosphate (T6P), are essential regulators of plant response to abiotic and biotic stress. Here we used the specific host-insect interaction between Linaria vulgaris (Plantaginaceae) and stem-galling weevil, Rhinusa pilosa (Mecinini, Curculionidae) with the aim to distinguish carbohydrate allocation patterns in response to herbivory, gall formation (G1, 24 h after oviposition), and gall development (G2, 7 days after oviposition) under controlled conditions. The hypothesis is that herbivory and galling induce distinct responses in both leaves and stems, and that shifts in carbon allocations are regulated by signaling sugars. Systemic response to herbivory was accumulation of T6P and maltose. The main feature of G1 in the stems was accumulation of trehalose, accompanied by increased T6P, turanose and glucose content, oppositely to the leaves. In G2, galls had 3-folds higher weight than controls, with further accumulation of fructose, glucose, turanose, and total water-insoluble carbohydrates (TIC), while the sucrose/hexose ratio decreased. Analysis of fast chlorophyll fluorescence kinetic (OJIP) transients in G2 showed a slight decrease in quantum yield of electron transport flux from Q(A) to Q(B), and towards photosystem I acceptor side, correlated with the decreased content of photosynthetic pigments and hexoses accumulation. Redistribution of photosynthates, and accumulation of T6P were induced in response to herbivory, indicating its signaling role. The results support the hypothesis that R. pilosa can induce plant reprogramming towards the accumulation of beneficial carbohydrates in developing gall by mechanisms which include both T6P and trehalose.",
publisher = "Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales",
journal = "Plant Physiology and Biochemistry",
title = "Resource allocation in response to herbivory and gall formation in Linaria vulgaris",
pages = "232-224",
volume = "135",
doi = "10.1016/j.plaphy.2018.11.032"
}

Sedlarević-Zorić, A., Morina, F., Toševski, I., Tosti, T., Jović, J., Krstić, O.,& Veljović-Jovanović, S.. (2019). Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry
Issy-Les-Moulineaux : Elsevier France - Editions Scientifiques Medicales., 135, 224-232.
https://doi.org/10.1016/j.plaphy.2018.11.032

Sedlarević-Zorić A, Morina F, Toševski I, Tosti T, Jović J, Krstić O, Veljović-Jovanović S. Resource allocation in response to herbivory and gall formation in Linaria vulgaris. in Plant Physiology and Biochemistry. 2019;135:224-232.
doi:10.1016/j.plaphy.2018.11.032 .

Sedlarević-Zorić, Ana, Morina, Filis, Toševski, Ivo, Tosti, Tomislav, Jović, Jelena, Krstić, Oliver, Veljović-Jovanović, Sonja, "Resource allocation in response to herbivory and gall formation in Linaria vulgaris" in Plant Physiology and Biochemistry, 135 (2019):224-232,
https://doi.org/10.1016/j.plaphy.2018.11.032 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/565
AB  - Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia
EP  - 195
IS  - 1
SP  - 183
VL  - 153
DO  - 10.1007/s10658-018-1553-1
ER  - 

@article{
author = "Kosovac, Andrea and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2019",
abstract = "Bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', is spreading and diversifying in several wine-producing regions of Europe, causing increased yield losses and economic concerns. The planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae) is a major vector of the pathogen that causes BN disease in grapevine. Recent studies suggest that epidemiology of BN is affected by the interaction of specific host races of H. obsoletus with weedy host plants that serve as reservoirs for the pathogen. Since BN is common in grape-growing regions of Serbia, a survey was conducted during 2015-2016 to evaluate the host-plant association and vector role of H. obsoletus in central and eastern regions. We tested populations of H. obsoletus associated with Convolvulus arvensis (field bindweed) a known host for the vector and pathogen and Crepis foetida (stinking hawk's-beard) a newly identified host in southeastern Europe. Molecular phylogenetic analyses of H. obsoletus collected from C. arvensis and C. foetida using the mtDNA COI marker determined that the two populations had a genetic divergence of 1.4%. Transmission trials confirmed the vector role of C. arvensis-associated H. obsoletus and identified C. foetida-associated populations as a new and efficient host-specialized vector of BN. Each of the two host-specialized vector populations transmitted Ca. P. solani' to its original host (C. arvensis or C. foetida) and to grapevine cv. Chardonnay. Multilocus genotyping of the transmitted phytoplasma isolates based on tuf/stamp/vmp1 Ca. P. solani'-specific loci indicated pathogen segregation according to the host-plant association of the vector. All isolates transmitted by C. foetida-associated H. obsoletus were of the STOL genotype (tuf-b/STOL/V2-TA), while the C. arvensis-associated population transmitted several genotypes: tuf-b/Rqg50/V4, tuf-b/Rqg50/V14, tuf-b/Rqg31/V14 and tuf-b/Vv24/V14. This study provides evidence of a new independent BN epidemiological cycle of tuf-b Ca. P. solani' strain in the Balkan region, sourced by C. foetida and transmitted by its associated H. obsoletus population.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia",
pages = "195-183",
number = "1",
volume = "153",
doi = "10.1007/s10658-018-1553-1"
}

Kosovac, A., Jakovljević, M., Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2019). Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 153(1), 183-195.
https://doi.org/10.1007/s10658-018-1553-1

Kosovac A, Jakovljević M, Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia. in European Journal of Plant Pathology. 2019;153(1):183-195.
doi:10.1007/s10658-018-1553-1 .

Kosovac, Andrea, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Role of plant-specialized Hyalesthes obsoletus associated with Convolvulus arvensis and Crepis foetida in the transmission of "Candidatus Phytoplasma solani'-inflicted bois noir disease of grapevine in Serbia" in European Journal of Plant Pathology, 153, no. 1 (2019):183-195,
https://doi.org/10.1007/s10658-018-1553-1 . .

TY  - JOUR
AU  - Hernandez-Vera, Gerardo
AU  - Toševski, Ivo
AU  - Caldara, Roberto
AU  - Emerson, Brent C.
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/564
AB  - Weevils (Coleoptera: Curculionoidea) represent one of the most diverse groups of organisms on Earth; interactions with their host plants have been recognized to play a central role in their remarkable diversity, yet the exact mechanisms and factors still remain poorly understood. Using phylogenetic comparative analyses, here we investigate the evolution of host use and its possible role in diversification processes of Rhinusa and Gymnetron, two closely related groups of weevils that feed and develop inside plant tissues of hosts within the families Scrophulariaceae and Plantaginaceae. We found strong evidence for phylogenetic conservatism of host use at the plant family level, most likely due to substantial differences in the chemical composition of hosts, reducing the probability of shifts between host families. In contrast, the use of different plant organs represents a more labile ecological trait and ecological niche expansion that allows a finer partitioning of resources. Rhinusa and Gymnetron weevils initially specialized on plants within Scrophulariaceae and then shifted to the closely related Plantaginaceae; likewise, a gall inducing behavior evolved from non-galler weevils, possibly in response to resource competition, as galls facilitate larval development by providing enhanced nutrition and a favorable microhabitat. Results from trait-dependent diversification analyses suggest that both use of hosts within Plantaginaceae and parasitism on fruits and seed capsules are associated with enhanced diversification of Rhinusa and Gymnetron via low extinction rates. Our study provides quantitative evidence and insights on the ecological factors that can promote diversification in phytophagous insects that feed and develop inside plant tissues.
PB  - PeerJ Inc.
T2  - PEERJ
T1  - Evolution of host plant use and diversification in a species complex of parasitic weevils (Coleoptera: Curculionidae)
VL  - 7
DO  - 10.7717/peerj.6625
ER  - 

@article{
author = "Hernandez-Vera, Gerardo and Toševski, Ivo and Caldara, Roberto and Emerson, Brent C.",
year = "2019",
abstract = "Weevils (Coleoptera: Curculionoidea) represent one of the most diverse groups of organisms on Earth; interactions with their host plants have been recognized to play a central role in their remarkable diversity, yet the exact mechanisms and factors still remain poorly understood. Using phylogenetic comparative analyses, here we investigate the evolution of host use and its possible role in diversification processes of Rhinusa and Gymnetron, two closely related groups of weevils that feed and develop inside plant tissues of hosts within the families Scrophulariaceae and Plantaginaceae. We found strong evidence for phylogenetic conservatism of host use at the plant family level, most likely due to substantial differences in the chemical composition of hosts, reducing the probability of shifts between host families. In contrast, the use of different plant organs represents a more labile ecological trait and ecological niche expansion that allows a finer partitioning of resources. Rhinusa and Gymnetron weevils initially specialized on plants within Scrophulariaceae and then shifted to the closely related Plantaginaceae; likewise, a gall inducing behavior evolved from non-galler weevils, possibly in response to resource competition, as galls facilitate larval development by providing enhanced nutrition and a favorable microhabitat. Results from trait-dependent diversification analyses suggest that both use of hosts within Plantaginaceae and parasitism on fruits and seed capsules are associated with enhanced diversification of Rhinusa and Gymnetron via low extinction rates. Our study provides quantitative evidence and insights on the ecological factors that can promote diversification in phytophagous insects that feed and develop inside plant tissues.",
publisher = "PeerJ Inc.",
journal = "PEERJ",
title = "Evolution of host plant use and diversification in a species complex of parasitic weevils (Coleoptera: Curculionidae)",
volume = "7",
doi = "10.7717/peerj.6625"
}

Hernandez-Vera, G., Toševski, I., Caldara, R.,& Emerson, B. C.. (2019). Evolution of host plant use and diversification in a species complex of parasitic weevils (Coleoptera: Curculionidae). in PEERJ
PeerJ Inc.., 7.
https://doi.org/10.7717/peerj.6625

Hernandez-Vera G, Toševski I, Caldara R, Emerson BC. Evolution of host plant use and diversification in a species complex of parasitic weevils (Coleoptera: Curculionidae). in PEERJ. 2019;7.
doi:10.7717/peerj.6625 .

Hernandez-Vera, Gerardo, Toševski, Ivo, Caldara, Roberto, Emerson, Brent C., "Evolution of host plant use and diversification in a species complex of parasitic weevils (Coleoptera: Curculionidae)" in PEERJ, 7 (2019),
https://doi.org/10.7717/peerj.6625 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/547
AB  - The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).
PB  - Public Library of Science (PLoS)
T2  - PlOS One
T1  - Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation
IS  - 5
VL  - 13
DO  - 10.1371/journal.pone.0196969
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The stolbur phytoplasma vector Hyalesthes obsoletus is generally considered as a polyphagous species associated with numerous wild and cultivated plants. However, recent research in southeastern Europe, the distribution centre of H. obsoletus and the area of most stolbur-inflicted crop diseases, points toward specific host-plant associations of the vector, indicating specific vector-based transmission routes. Here, we study the specificity of populations associated with four host-plants using mitochondrial and nuclear genetic markers, and we evaluate the evolution of host-shifts in H. obsoletus. Host-plant use was confirmed for Convolvulus arvensis, Urtica dioica, Vitex agnus-castus and Crepis foetida. Mitochondrial genetic analysis showed sympatric occurrence of three phylogenetic lineages that were ecologically delineated by host-plant preference, but were morphologically inseparable. Nuclear data supported the existence of three genetic groups (Evanno's Delta K(3) = 803.72) with average genetic membership probabilities  gt  90%. While populations associated with C. arvensis and U. dioica form a homogenous group, populations affiliated with V. agnus-castus and C. foetida constitute two independent plant-associated lineages. The geographical signal permeating the surveyed populations indicated complex diversification processes associated with host-plant selection and likely derived from post-glacial refugia in the eastern Mediterranean. This study provides evidence for cryptic species diversification within H. obsoletus sensu lato: i) consistent mitochondrial differentiation (1.1-1.5%) among host-associated populations in syntopy and in geographically distant areas, ii) nuclear genetic variance supporting mitochondrial data, and iii) average mitochondrial genetic distances among host-associated meta-populations are comparable to the most closely related, morphologically distinguishable species, i.e., Hyalesthes thracicus (2.1-3.3%).",
publisher = "Public Library of Science (PLoS)",
journal = "PlOS One",
title = "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation",
number = "5",
volume = "13",
doi = "10.1371/journal.pone.0196969"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2018). Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One
Public Library of Science (PLoS)., 13(5).
https://doi.org/10.1371/journal.pone.0196969

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation. in PlOS One. 2018;13(5).
doi:10.1371/journal.pone.0196969 .

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Widespread plant specialization in the polyphagous planthopper Hyalesthes obsoletus (Cixiidae), a major vector of stolbur phytoplasma: Evidence of cryptic speciation" in PlOS One, 13, no. 5 (2018),
https://doi.org/10.1371/journal.pone.0196969 . .

TY  - JOUR
AU  - Skuhrovec, Jiri
AU  - Gosik, Rafal
AU  - Caldara, Roberto
AU  - Toševski, Ivo
AU  - Letowski, Jacek
AU  - Szwaj, Ewelina
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/525
AB  - The relationship between the genera Cleopomiarus and Miarus of Mecinini (Curculionidae, Curculioninac) was tested on the basis of morphological characters from the immature stages. The mature larvae of five Cleopomiarus species (C. distinctus (Boheman, 1845), C. graminis (Gyllenhal, 1813), C. longirostris (Gyllenhal, 1838), C. medius (Desbrochers des Loges, 1893), and C. meridionalis (H. Brisout de Barneville, 1863)), three Miarus species (M. abnormis Solari, 1947, M. ajugae (Herbst, 1795), and M. campanulae (Linnaeus, 1767)), and the pupae of four Cleopomiarus species (C. distinct us, C. graminis, C. longirostris, and C. medius) and two Miarus species (M. abnormis and M. ajugae) are described in detail for the first time. To confirm the taxonomic identification of some larvae, DNA COI barcode was obtained and compared with those of adults. The immature stages of the species herein studied were compared with those known from other genera in tribe Mecinini. It is suggested that Miarus and Cleopomiarus may be monophyletic based on several shared distinctive characters. Larvae of Miarus have a characteristic maxil-lary mala with six finger-like dms of two sizes (one or two elms very long and the rest of medium length), this feature being apparently unique among weevils. Other genus-specific character states are observed in the pupae, such as the length of setae on the head, rostrum and pronotum, including the number of rs on the rostrum, ds on pronotum, and finally the shape of the urogomphi. A key to the described larvae and pupae were respectively presented. New biological and distributional data on some species are reported.
PB  - Pensoft Publishers
T2  - Zookeys
T1  - Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae)
EP  - 92
IS  - 808
SP  - 23
DO  - 10.3897/zookeys.808.28172
ER  - 

@article{
author = "Skuhrovec, Jiri and Gosik, Rafal and Caldara, Roberto and Toševski, Ivo and Letowski, Jacek and Szwaj, Ewelina",
year = "2018",
abstract = "The relationship between the genera Cleopomiarus and Miarus of Mecinini (Curculionidae, Curculioninac) was tested on the basis of morphological characters from the immature stages. The mature larvae of five Cleopomiarus species (C. distinctus (Boheman, 1845), C. graminis (Gyllenhal, 1813), C. longirostris (Gyllenhal, 1838), C. medius (Desbrochers des Loges, 1893), and C. meridionalis (H. Brisout de Barneville, 1863)), three Miarus species (M. abnormis Solari, 1947, M. ajugae (Herbst, 1795), and M. campanulae (Linnaeus, 1767)), and the pupae of four Cleopomiarus species (C. distinct us, C. graminis, C. longirostris, and C. medius) and two Miarus species (M. abnormis and M. ajugae) are described in detail for the first time. To confirm the taxonomic identification of some larvae, DNA COI barcode was obtained and compared with those of adults. The immature stages of the species herein studied were compared with those known from other genera in tribe Mecinini. It is suggested that Miarus and Cleopomiarus may be monophyletic based on several shared distinctive characters. Larvae of Miarus have a characteristic maxil-lary mala with six finger-like dms of two sizes (one or two elms very long and the rest of medium length), this feature being apparently unique among weevils. Other genus-specific character states are observed in the pupae, such as the length of setae on the head, rostrum and pronotum, including the number of rs on the rostrum, ds on pronotum, and finally the shape of the urogomphi. A key to the described larvae and pupae were respectively presented. New biological and distributional data on some species are reported.",
publisher = "Pensoft Publishers",
journal = "Zookeys",
title = "Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae)",
pages = "92-23",
number = "808",
doi = "10.3897/zookeys.808.28172"
}

Skuhrovec, J., Gosik, R., Caldara, R., Toševski, I., Letowski, J.,& Szwaj, E.. (2018). Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae). in Zookeys
Pensoft Publishers.(808), 23-92.
https://doi.org/10.3897/zookeys.808.28172

Skuhrovec J, Gosik R, Caldara R, Toševski I, Letowski J, Szwaj E. Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae). in Zookeys. 2018;(808):23-92.
doi:10.3897/zookeys.808.28172 .

Skuhrovec, Jiri, Gosik, Rafal, Caldara, Roberto, Toševski, Ivo, Letowski, Jacek, Szwaj, Ewelina, "Morphological characters of immature stages of Palaearctic species of Cleopomiarus and Miarus and their systematic value in Mecinini (Coleoptera, Curculionidae, Curculioninae)" in Zookeys, no. 808 (2018):23-92,
https://doi.org/10.3897/zookeys.808.28172 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/550
AB  - The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions
EP  - 64
IS  - 1
SP  - 47
VL  - 172
DO  - 10.1111/aab.12400
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Radonjić, Sanja and Hrnčić, Snježana and Toševski, Ivo and Jović, Jelena",
year = "2018",
abstract = "The European lantern fly, Dictyophara europaea, is an alternative vector of the Flavescence doree phytoplasma (FDp) disease of grapevine in European vineyards, enabling infection initiation from wild reservoir compartment (Clematis vitalba). Heretofore recorded rate of D. europaea FDp-infection has been very low (3%), making it less epidemiologically significant than would be expected based on reservoir plant infection rate (30%). In this study we present findings on a heavily FDp-infected D. europaea population ( gt 60%), on the natural Wolbachia infection of populations with low FDp-infection rates (DeWo+) and on Wolbachia absence in highly FDp-infected population (DeWo-). We examine several possible causes underlying the differences in vector infection rates: (a) population genetic characteristics of D. europaea and correlation with Wolbachia strain wEur natural infections, (b) Wolbachia effects on fitness components of DeWo+ laboratory colony and (c) rate of reservoir plant FDp-infection and differences in FDp genotypes harboured by low and highly infected vector populations. The vector genetic diversity level was found to be lower in DeWo+ than in uninfected individuals and to exhibit a different evolution of fixed haplotypes. All DeWo+ populations were infected with the same strain of wEur. The FDp was found to be genetically diversified (five genotypes) but had no relation to infection rates. We did not find evidence of fitness upgrades with regard to Wolbachia infection status. Although more experimentation is needed, it seems that Wolbachia confers protection against FDp or is in competition with FDp according to the observed correlations: low FDp-infected vector populations are infected with Wolbachia and vice versa.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions",
pages = "64-47",
number = "1",
volume = "172",
doi = "10.1111/aab.12400"
}

Krstić, O., Cvrković, T., Mitrović, M., Radonjić, S., Hrnčić, S., Toševski, I.,& Jović, J.. (2018). Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology
Wiley, Hoboken., 172(1), 47-64.
https://doi.org/10.1111/aab.12400

Krstić O, Cvrković T, Mitrović M, Radonjić S, Hrnčić S, Toševski I, Jović J. Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions. in Annals of Applied Biology. 2018;172(1):47-64.
doi:10.1111/aab.12400 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Radonjić, Sanja, Hrnčić, Snježana, Toševski, Ivo, Jović, Jelena, "Wolbachia infection in natural populations of Dictyophara europaea, an alternative vector of grapevine Flavescence doree phytoplasma: effects and interactions" in Annals of Applied Biology, 172, no. 1 (2018):47-64,
https://doi.org/10.1111/aab.12400 . .

TY  - JOUR
AU  - Toševski, Ivo
AU  - Sing, Sharlene
AU  - De Clerck-Floate, Rosemarie
AU  - McClay, A.
AU  - Weaver, D. K.
AU  - Schwarzlander, M.
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Gassmann, Andre
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/549
AB  - Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.
PB  - Wiley, Hoboken
T2  - Annals of Applied Biology
T1  - Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America
EP  - 34
IS  - 1
SP  - 16
VL  - 173
DO  - 10.1111/aab.12430
ER  - 

@article{
author = "Toševski, Ivo and Sing, Sharlene and De Clerck-Floate, Rosemarie and McClay, A. and Weaver, D. K. and Schwarzlander, M. and Krstić, Oliver and Jović, Jelena and Gassmann, Andre",
year = "2018",
abstract = "Linaria vulgaris, common or yellow toadflax, and Linaria dalmatica, Dalmatian toadflax (Plantaginaceae), are Eurasian perennial forbs invasive throughout temperate North America. These Linaria species have been the targets of classical biological control programmes in Canada and the USA since the 1960s. The first effective toadflax biological control agent, the stem-mining weevil Mecinus janthinus (Coleoptera: Curculionidae) was introduced from Europe in the 1990s. This weevil has become established on L. dalmatica and L. vulgaris in both countries, although it has shown greater success in controlling the former toadflax species. Genetic and ecological studies of native range M. janthinus populations revealed that weevils previously identified as a single species in fact include two cryptic species, now recognised as M. janthinus, associated with yellow toadflax, and the recently confirmed species Mecinus janthiniformis, associated with Dalmatian toadflax. The results of a comprehensive study characterising haplotype identities, distributions and frequencies within M. janthinus s.l. native range source populations were compared to those populations currently established in the USA and Canada. The presence of both Mecinus species in North America was confirmed, and revealed with a few exceptions a high and consistent level of host fidelity throughout the adopted and native ranges. Genetic analysis based on mitochondrial cytochrome oxidase subunit II gene (mtCOII) defined the origin and records the subsequent North American establishment, by haplotype, of the European founder populations of M. janthinus (northern Switzerland and southern Germany) and M. janthiniformis (southern Macedonia), and provided population genetic indices for the studied populations. This analysis together with existing North American shipment receipt, release and rearing records elucidates probable redistribution routes and sources of both weevil species from initially released and established adopted range populations.",
publisher = "Wiley, Hoboken",
journal = "Annals of Applied Biology",
title = "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America",
pages = "34-16",
number = "1",
volume = "173",
doi = "10.1111/aab.12430"
}

Toševski, I., Sing, S., De Clerck-Floate, R., McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, O., Jović, J.,& Gassmann, A.. (2018). Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology
Wiley, Hoboken., 173(1), 16-34.
https://doi.org/10.1111/aab.12430

Toševski I, Sing S, De Clerck-Floate R, McClay A, Weaver DK, Schwarzlander M, Krstić O, Jović J, Gassmann A. Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America. in Annals of Applied Biology. 2018;173(1):16-34.
doi:10.1111/aab.12430 .

Toševski, Ivo, Sing, Sharlene, De Clerck-Floate, Rosemarie, McClay, A., Weaver, D. K., Schwarzlander, M., Krstić, Oliver, Jović, Jelena, Gassmann, Andre, "Twenty-five years after: post-introduction association of Mecinus janthinus s.l. with invasive host toadflaxes Linaria vulgaris and Linaria dalmatica in North America" in Annals of Applied Biology, 173, no. 1 (2018):16-34,
https://doi.org/10.1111/aab.12430 . .

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Mitrović, Milana
AU  - Dietrich, Christopher H.
AU  - Toševski, Ivo
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/477
AB  - The introduction of invasive species may result in important ecological, environmental, and economic impacts. Extensive study of Auchenorrhyncha (Hemiptera) fauna in a wine-growing region in southern Switzerland revealed, for the first time, presence of the Nearctic leafhopper Osbornellus auronitens (Provancher) (Hemiptera: Cicadellidae) in the vicinity of Stabio (Canton of Ticino). The species identity of the collected specimens was confirmed using morphological and molecular characters. All specimens of O. auronitens were collected in a forest of Castanea sativa Miller (Fagaceae), Corylus avellana Linnaeus (Betulaceae), and Alnus glutinosa (Linnaeus) Gaertner (Betulaceae) intermixed with Cornus sanguinea Linnaeus (Cornaceae), Salix Linnaeus (Salicaceae), and Rubus Linnaeus (Rosaceae). In its native range this leafhopper is polyphagous and a relatively common visitor in vineyards. Based on analysis of the barcoding region of the mitochondrial cytochrome oxidase subunit I gene of the collected O. auronitens, 100% identity with specimens of the same species originating from Canada was determined. Osbornellus auronitens is morphologically similar to Scaphoideus titanus Ball (Hemiptera: Cicadellidae), another introduced Nearctic leafhopper, which was involved in severe outbreaks of disease caused by the Grapevine flavescence doree phytoplasma (Bacteria: Acholeplasmataceae) in European viticultural regions since the 1960s. In this paper, we report the morphological features to distinguish O. auronitens from S. titanus, and discuss the possible implications of its expected spread across the Old World.
PB  - Cambridge Univ Press, New York
T2  - Canadian Entomologist
T1  - Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region
EP  - 559
IS  - 5
SP  - 551
VL  - 149
DO  - 10.4039/tce.2017.7
ER  - 

@article{
author = "Trivellone, Valeria and Mitrović, Milana and Dietrich, Christopher H. and Toševski, Ivo",
year = "2017",
abstract = "The introduction of invasive species may result in important ecological, environmental, and economic impacts. Extensive study of Auchenorrhyncha (Hemiptera) fauna in a wine-growing region in southern Switzerland revealed, for the first time, presence of the Nearctic leafhopper Osbornellus auronitens (Provancher) (Hemiptera: Cicadellidae) in the vicinity of Stabio (Canton of Ticino). The species identity of the collected specimens was confirmed using morphological and molecular characters. All specimens of O. auronitens were collected in a forest of Castanea sativa Miller (Fagaceae), Corylus avellana Linnaeus (Betulaceae), and Alnus glutinosa (Linnaeus) Gaertner (Betulaceae) intermixed with Cornus sanguinea Linnaeus (Cornaceae), Salix Linnaeus (Salicaceae), and Rubus Linnaeus (Rosaceae). In its native range this leafhopper is polyphagous and a relatively common visitor in vineyards. Based on analysis of the barcoding region of the mitochondrial cytochrome oxidase subunit I gene of the collected O. auronitens, 100% identity with specimens of the same species originating from Canada was determined. Osbornellus auronitens is morphologically similar to Scaphoideus titanus Ball (Hemiptera: Cicadellidae), another introduced Nearctic leafhopper, which was involved in severe outbreaks of disease caused by the Grapevine flavescence doree phytoplasma (Bacteria: Acholeplasmataceae) in European viticultural regions since the 1960s. In this paper, we report the morphological features to distinguish O. auronitens from S. titanus, and discuss the possible implications of its expected spread across the Old World.",
publisher = "Cambridge Univ Press, New York",
journal = "Canadian Entomologist",
title = "Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region",
pages = "559-551",
number = "5",
volume = "149",
doi = "10.4039/tce.2017.7"
}

Trivellone, V., Mitrović, M., Dietrich, C. H.,& Toševski, I.. (2017). Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region. in Canadian Entomologist
Cambridge Univ Press, New York., 149(5), 551-559.
https://doi.org/10.4039/tce.2017.7

Trivellone V, Mitrović M, Dietrich CH, Toševski I. Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region. in Canadian Entomologist. 2017;149(5):551-559.
doi:10.4039/tce.2017.7 .

Trivellone, Valeria, Mitrović, Milana, Dietrich, Christopher H., Toševski, Ivo, "Osbornellus auronitens (Hemiptera: Cicadellidae: Deltocephalinae), an introduced species new for the Palaearctic region" in Canadian Entomologist, 149, no. 5 (2017):551-559,
https://doi.org/10.4039/tce.2017.7 . .

TY  - JOUR
AU  - Trkulja, Nenad
AU  - Milosavljević, Anja
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Khan, Mohamed F. R.
AU  - Secor, Gary A.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/475
AB  - Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides
EP  - 910
IS  - 4
SP  - 895
VL  - 149
DO  - 10.1007/s10658-017-1239-0
ER  - 

@article{
author = "Trkulja, Nenad and Milosavljević, Anja and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Khan, Mohamed F. R. and Secor, Gary A.",
year = "2017",
abstract = "Cercospora leaf spot (CLS) caused by Cercospora beticola occurs annually in Serbia causing severe yield losses of sugar beet, which requires intensive use of fungicides. In recent years we have observed unsatisfactory control of CLS originating from northwestern Serbia. Frequency of C. beticola populations resistant to Quinone outside inhibitors (QoI) was 81% (51/63 isolates), 98% (62/63) to sterol-demethylation inbibitors (DMI) and 54% (34/63) to methyl-2-benzimidazole carbamate fungicides (MBC). The genetic basis underlying the resistance was tested by characterizing the cob, CYP51 and -tubulin genes, associated with resistance to QoI, DMI and MBC fungicides, respectively. Isolates that were resistant to QoI fungicides had the G143A mutation in the cob gene. Characterization of the CYP51 gene revealed seven diverse haplotypes; however, no correlation with sensitivity or resistance to DMI fungicides could be identified. Resistance to MBC fungicides was associated with the presence of the E198A mutation in the -tubulin gene of all resistant isolates. From a total of 63 isolates originating from sugar beet fields of northwestern Serbia, 62 isolates showed resistance to multiple modes of action. Three multi-resistant phenotypes were identified: MR1 (N = 29) - resistant to QoI and DMI fungicides (QoI-R and DMI-R) but sensitive to MBC fungicides (MBC-S); MR2 (N = 11, QoI-S, DMI-R and MBC-R); and MR3 (N = 22), resistant to all three groups of fungicides (QoI-R, DMI-R and MBC-R). This is the first report of C. beticola resistance to QoI fungicides in Serbia. This study revealed development of multi-resistance of C. beticola isolates to MBC, DMI and QoI fungicides, which represents the first record of this phenomenon in C. beticola populations.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides",
pages = "910-895",
number = "4",
volume = "149",
doi = "10.1007/s10658-017-1239-0"
}

Trkulja, N., Milosavljević, A., Mitrović, M., Jović, J., Toševski, I., Khan, M. F. R.,& Secor, G. A.. (2017). Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology
Springer, Dordrecht., 149(4), 895-910.
https://doi.org/10.1007/s10658-017-1239-0

Trkulja N, Milosavljević A, Mitrović M, Jović J, Toševski I, Khan MFR, Secor GA. Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides. in European Journal of Plant Pathology. 2017;149(4):895-910.
doi:10.1007/s10658-017-1239-0 .

Trkulja, Nenad, Milosavljević, Anja, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Khan, Mohamed F. R., Secor, Gary A., "Molecular and experimental evidence of multi-resistance of Cercospora beticola field populations to MBC, DMI and QoI fungicides" in European Journal of Plant Pathology, 149, no. 4 (2017):895-910,
https://doi.org/10.1007/s10658-017-1239-0 . .

TY  - JOUR
AU  - Gosik, Rafal
AU  - Skuhrovec, Jiri
AU  - Toševski, Ivo
AU  - Caldara, Roberto
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/489
AB  - The relationship between the subtribes Lignyodina and Tychiina of the Tychiini were tested on the basis of morphological characters from the immature stages. The mature larvae of Lignyodes bischoffi (Blatchley, 1916) and L. enucleator (Panzer, 1798) (Curculionidae, Curculioninae, Tychiini, Lignyodina) and the pupae of L. bischoffi are described in detail for the first time. To be sure about the correct taxonomic identification of the larvae, DNA sequences were obtained and compared for both the larvae and adults of these two species. Molecular data show that the two studied species of Lignyodes Dejean, 1835 strongly differ in mitochondrial COI and 16S rRNA. The immature stages (larva and pupa) of Lignyodes bischoffi and L. enucleator were compared with each other and with those known for other taxa representative of the tribe Tychiini and other tribes of Curculioninae. The larvae of Lignyodes bischoffi and L. enucleator differ in a few but important characters. The larvae and pupae of these two species belonging to Lignyodes s. str. are morphologically very similar to those of species in the other two subgenera of Lignyodes, Neotylopterus Clark, Whitehead & Warner, 1977 and Chionanthobius Pierce, 1912. On the basis of the detailed morphology of larvae and pupae, we agree that Lignyodes belong to Tychiini, sharing more features with species of this tribe than with those of other tribes of Curculioninae. New bionomic data concerning larval and pupal development and adult emergence are reported for both described species and confirm close relationships between the subtribes Lignyodina and Tychiina. On the other hand, strong molecular differences, corroborated by some relevant larval differences, between L. enucleator and L. bischoffi open up the issue whether Palaearctic and Nearctic species presently included in Lignyodes s. str. actually belong to the same subgenus.
PB  - Magnolia Press, Auckland
T2  - Zootaxa
T1  - Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini)
EP  - 446
IS  - 3
SP  - 426
VL  - 4320
DO  - 10.11646/zootaxa.4320.3.2
ER  - 

@article{
author = "Gosik, Rafal and Skuhrovec, Jiri and Toševski, Ivo and Caldara, Roberto",
year = "2017",
abstract = "The relationship between the subtribes Lignyodina and Tychiina of the Tychiini were tested on the basis of morphological characters from the immature stages. The mature larvae of Lignyodes bischoffi (Blatchley, 1916) and L. enucleator (Panzer, 1798) (Curculionidae, Curculioninae, Tychiini, Lignyodina) and the pupae of L. bischoffi are described in detail for the first time. To be sure about the correct taxonomic identification of the larvae, DNA sequences were obtained and compared for both the larvae and adults of these two species. Molecular data show that the two studied species of Lignyodes Dejean, 1835 strongly differ in mitochondrial COI and 16S rRNA. The immature stages (larva and pupa) of Lignyodes bischoffi and L. enucleator were compared with each other and with those known for other taxa representative of the tribe Tychiini and other tribes of Curculioninae. The larvae of Lignyodes bischoffi and L. enucleator differ in a few but important characters. The larvae and pupae of these two species belonging to Lignyodes s. str. are morphologically very similar to those of species in the other two subgenera of Lignyodes, Neotylopterus Clark, Whitehead & Warner, 1977 and Chionanthobius Pierce, 1912. On the basis of the detailed morphology of larvae and pupae, we agree that Lignyodes belong to Tychiini, sharing more features with species of this tribe than with those of other tribes of Curculioninae. New bionomic data concerning larval and pupal development and adult emergence are reported for both described species and confirm close relationships between the subtribes Lignyodina and Tychiina. On the other hand, strong molecular differences, corroborated by some relevant larval differences, between L. enucleator and L. bischoffi open up the issue whether Palaearctic and Nearctic species presently included in Lignyodes s. str. actually belong to the same subgenus.",
publisher = "Magnolia Press, Auckland",
journal = "Zootaxa",
title = "Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini)",
pages = "446-426",
number = "3",
volume = "4320",
doi = "10.11646/zootaxa.4320.3.2"
}

Gosik, R., Skuhrovec, J., Toševski, I.,& Caldara, R.. (2017). Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini). in Zootaxa
Magnolia Press, Auckland., 4320(3), 426-446.
https://doi.org/10.11646/zootaxa.4320.3.2

Gosik R, Skuhrovec J, Toševski I, Caldara R. Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini). in Zootaxa. 2017;4320(3):426-446.
doi:10.11646/zootaxa.4320.3.2 .

Gosik, Rafal, Skuhrovec, Jiri, Toševski, Ivo, Caldara, Roberto, "Morphological evidence from immature stages further suggests Lignyodina being close to Tychiina (Coleoptera, Curculionidae, Curculioninae, Tychiini)" in Zootaxa, 4320, no. 3 (2017):426-446,
https://doi.org/10.11646/zootaxa.4320.3.2 . .

TY  - JOUR
AU  - Stepanović, Svetlana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/445
AB  - Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.
PB  - Wiley, Hoboken
T2  - Insect Science
T1  - Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification
EP  - 648
IS  - 4
SP  - 638
VL  - 23
DO  - 10.1111/1744-7917.12212
ER  - 

@article{
author = "Stepanović, Svetlana and Kosovac, Andrea and Krstić, Oliver and Jović, Jelena and Toševski, Ivo",
year = "2016",
abstract = "Genotyping of 2 well-known weevil species from the genus Ceutorhynchus (Coleoptera: Curculionidae) distributed in west Palearctic, C. erysimi and C. contractus, revealed phenotype versus genotype inconsistencies in a set of 56 specimens (25 C. erysimi and 31 C. contractus) collected from 25 locations in Serbia and Montenegro. An analysis of the mitochondrial cytochrome oxidase subunit I gene (COI), widely used as a barcoding region, and a nuclear gene, elongation factor-1 alpha (EF-1 alpha), revealed stable genetic divergence among these species. The average uncorrected pairwise distances for the COI and EF-1 alpha genes were 3.8%, and 1.3%, respectively, indicating 2 genetically well-segregated species. However, the genetic data were not congruent with the phenotypic characteristics of the studied specimens. In the first place, C. erysimi genotypes were attached to specimens with phenotypic characteristics of C. contractus. Species-specific PCR-RFLP assays for the barcoding gene COIwere applied for themolecular identification of 101 additional specimens of both morphospecies (33 C. erysimi and 68 C. contractus) and were found to confirm this incongruity. The discrepancy between the genetic and morphological data raises the question of the accuracy of using a barcoding approach, as it may result in misleading conclusions about the taxonomic position of the studied organism. Additionally, the typological species concept shows considerable weakness when genetic data are not supported with phenotypic characteristics as in case of asymmetric introgression, which may cause certain problems, especially in applied studies such as biological control programs in which the biological properties of the studied organisms are the main focus.",
publisher = "Wiley, Hoboken",
journal = "Insect Science",
title = "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification",
pages = "648-638",
number = "4",
volume = "23",
doi = "10.1111/1744-7917.12212"
}

Stepanović, S., Kosovac, A., Krstić, O., Jović, J.,& Toševski, I.. (2016). Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science
Wiley, Hoboken., 23(4), 638-648.
https://doi.org/10.1111/1744-7917.12212

Stepanović S, Kosovac A, Krstić O, Jović J, Toševski I. Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification. in Insect Science. 2016;23(4):638-648.
doi:10.1111/1744-7917.12212 .

Stepanović, Svetlana, Kosovac, Andrea, Krstić, Oliver, Jović, Jelena, Toševski, Ivo, "Morphology versus DNA barcoding: two sides of the same coin. A case study of Ceutorhynchus erysimi and C. contractus identification" in Insect Science, 23, no. 4 (2016):638-648,
https://doi.org/10.1111/1744-7917.12212 . .

TY  - JOUR
AU  - Sedlarević, Ana
AU  - Morina, Filis
AU  - Toševski, Ivo
AU  - Gašić, Uroš
AU  - Natić, Maja
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/443
AB  - Rhinusa pilosa (Gyllenhal) is a highly specific weevil that induces stem galls on the common toadflax Linaria vulgaris Mill. females oviposit the eggs near the apex of a growing shoot. The act of oviposition is accompanied by secretion of an ovipositional fluid, which is considered to be cecidogen, directly involved in gall induction. The remains of cecidogenic fluid were collected from the surface of the oviposition point on the stem. We performed a comparative analysis of the phenolics extracted from cecidogen, the stem and galls of L. vulgaris and adult and larva of R. pilosa by HPLC-DAD. One compound with A (max) at 273, 332 nm (R (t) 30.65 min) was exclusively found in the methanol extract of cecidogen. To further characterize the cecidogen and stem phenolic profiles, we used UHPLC coupled with an OrbiTrap mass analyzer. Among 49 phenolic compounds extracted from both the ovipositional fluid and the plant, protocatechuic acid and two phenolic glycosides were exclusively found in cecidogen: diosmetin-O-acetylrutinoside and an unidentified compound. The unknown compound produced an MS2 base peak at 387 and 327 and 267 m/z base peaks at MS3 and MS4 fragmentation, respectively, and had the molecular formula C32H31O18. The plausible role of phenolic compounds in the induction of gall formation on L. vulgaris is discussed.
PB  - Springer, Dordrecht
T2  - Arthropod-Plant Interactions
T1  - Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)
EP  - 322
IS  - 4
SP  - 311
VL  - 10
DO  - 10.1007/s11829-016-9435-y
ER  - 

@article{
author = "Sedlarević, Ana and Morina, Filis and Toševski, Ivo and Gašić, Uroš and Natić, Maja and Jović, Jelena and Krstić, Oliver and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Rhinusa pilosa (Gyllenhal) is a highly specific weevil that induces stem galls on the common toadflax Linaria vulgaris Mill. females oviposit the eggs near the apex of a growing shoot. The act of oviposition is accompanied by secretion of an ovipositional fluid, which is considered to be cecidogen, directly involved in gall induction. The remains of cecidogenic fluid were collected from the surface of the oviposition point on the stem. We performed a comparative analysis of the phenolics extracted from cecidogen, the stem and galls of L. vulgaris and adult and larva of R. pilosa by HPLC-DAD. One compound with A (max) at 273, 332 nm (R (t) 30.65 min) was exclusively found in the methanol extract of cecidogen. To further characterize the cecidogen and stem phenolic profiles, we used UHPLC coupled with an OrbiTrap mass analyzer. Among 49 phenolic compounds extracted from both the ovipositional fluid and the plant, protocatechuic acid and two phenolic glycosides were exclusively found in cecidogen: diosmetin-O-acetylrutinoside and an unidentified compound. The unknown compound produced an MS2 base peak at 387 and 327 and 267 m/z base peaks at MS3 and MS4 fragmentation, respectively, and had the molecular formula C32H31O18. The plausible role of phenolic compounds in the induction of gall formation on L. vulgaris is discussed.",
publisher = "Springer, Dordrecht",
journal = "Arthropod-Plant Interactions",
title = "Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)",
pages = "322-311",
number = "4",
volume = "10",
doi = "10.1007/s11829-016-9435-y"
}

Sedlarević, A., Morina, F., Toševski, I., Gašić, U., Natić, M., Jović, J., Krstić, O.,& Veljović-Jovanović, S.. (2016). Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae). in Arthropod-Plant Interactions
Springer, Dordrecht., 10(4), 311-322.
https://doi.org/10.1007/s11829-016-9435-y

Sedlarević A, Morina F, Toševski I, Gašić U, Natić M, Jović J, Krstić O, Veljović-Jovanović S. Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae). in Arthropod-Plant Interactions. 2016;10(4):311-322.
doi:10.1007/s11829-016-9435-y .

Sedlarević, Ana, Morina, Filis, Toševski, Ivo, Gašić, Uroš, Natić, Maja, Jović, Jelena, Krstić, Oliver, Veljović-Jovanović, Sonja, "Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)" in Arthropod-Plant Interactions, 10, no. 4 (2016):311-322,
https://doi.org/10.1007/s11829-016-9435-y . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/428
AB  - A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors
EP  - 630
IS  - 3
SP  - 619
VL  - 144
DO  - 10.1007/s10658-015-0800-y
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Jermini, Mauro and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
abstract = "A progressive spread of stolbur-associated symptoms observed in potato fields in Serbia over the past few years initiated the study on disease epidemiology and transmission pathways performed during 2013 and 2014. Inspection of potato fields on 12 localities in northern Serbia revealed high incidence (60 % of symptomatic plants) and wide dispersal (100 % of inspected localities) of 'Candidatus Phytoplasma solani'. A qualitative analysis of Auchenorrhyncha fauna in affected potato fields identified 16 species, however only Hyalesthes obsoletus, Reptalus panzeri and R. quinquecostatus tested positive for 'Ca. P. solani'. Multilocus typing of strains associated with field collected potato plants and insects had been performed to identify the propagation scenario underlying the threatening epidemics. Combined analyses of the tuf, stamp and vmp1 genes detected ten genotypes, seven of which were shared by the potato plants and insects, confirming their interaction, with no clear species-specific association of certain 'Ca. P. solani' genotypes with plausible insect vectors. Semi- field experiments with naturally 'Ca. P. solani'-infected H. obsoletus and R. panzeri confirmed the ability of both species to successfully transmit the pathogen to potato plants and induce symptoms characteristic of stolbur disease. The third putative vector R. quinquecostatus shared genotypes of 'Ca. P. solani' with potato plants and other two cixiids, and though not tested in this study should not be ruled out as a potential vector. Our study revealed rather complex epidemiology of potato stolbur in Serbia involving several possible routes of horizontal transmission and provided experimental evidence for two natural planthopper vectors.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors",
pages = "630-619",
number = "3",
volume = "144",
doi = "10.1007/s10658-015-0800-y"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Jermini, M., Toševski, I.,& Cvrković, T.. (2016). 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology
Springer, Dordrecht., 144(3), 619-630.
https://doi.org/10.1007/s10658-015-0800-y

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Jermini M, Toševski I, Cvrković T. 'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors. in European Journal of Plant Pathology. 2016;144(3):619-630.
doi:10.1007/s10658-015-0800-y .

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Jermini, Mauro, Toševski, Ivo, Cvrković, Tatjana, "'Candidatus phytoplasma solani' genotypes associated with potato stolbur in Serbia and the role of Hyalesthes obsoletus and Reptalus panzeri (hemiptera, cixiidae) as natural vectors" in European Journal of Plant Pathology, 144, no. 3 (2016):619-630,
https://doi.org/10.1007/s10658-015-0800-y . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/439
AB  - Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)
EP  - 298
IS  - 2
SP  - 285
VL  - 65
DO  - 10.1111/ppa.12409
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "Epidemiological aspects and transmission routes of bois noir (BN), a grapevine yellows disease induced by Candidatus Phytoplasma solani', have been exhaustively studied in the affected vineyards of continental Europe but not in the Mediterranean coastal zone. Because Ca. Phytoplasma solani' and its principal vector Hyalesthes obsoletus presumably originate from the Mediterranean, gaining knowledge of the epidemiological peculiarities of the disease in this area is essential for understanding its global spread and diversification, as well as for designing local management strategies. In this study, molecular epidemiology was applied to trace transmission pathways of Ca. Phytoplasma solani' in the Mediterranean vineyards of Montenegro, using multilocus sequence typing of tuf, vmp1 and stamp genes of the isolates associated with various hosts. Thus, Ca. Phytoplasma solani' was tracked from a tentative reservoir plant (inoculum source) through an associated vector population to the infected grapevine. Three pathways of transmission were documented, originating from Urtica dioica, Convolvulus arvensis and Vitex agnus-castus; however, only the route originating from U.dioica was direct, whereas the latter two were overlapping and could be intermixed. Vitex agnus-castus is a natural source of Ca. Phytoplasma solani', representing an important link in disease epidemiology in the Mediterranean and a possible origin of several genotypes occurring in central Europe. Experimental confirmation of the role of Vitex-associated H.obsoletus in BN transmission in Montenegrin vineyards indicates its tentative role as a vector in the wide area of the Mediterranean, where some of the major wine-producing regions are located.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)",
pages = "298-285",
number = "2",
volume = "65",
doi = "10.1111/ppa.12409"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology
Wiley, Hoboken., 65(2), 285-298.
https://doi.org/10.1111/ppa.12409

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae). in Plant Pathology. 2016;65(2):285-298.
doi:10.1111/ppa.12409 .

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "Molecular tracing of the transmission routes of bois noir in Mediterranean vineyards of Montenegro and experimental evidence for the epidemiological role of Vitex agnus-castus (Lamiaceae) and associated Hyalesthes obsoletus (Cixiidae)" in Plant Pathology, 65, no. 2 (2016):285-298,
https://doi.org/10.1111/ppa.12409 . .

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/442
AB  - The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations
EP  - 405
IS  - 3
SP  - 395
VL  - 106
DO  - 10.1017/S0007485316000067
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
abstract = "The European lantern fly Dictyophara europaea (Linnaeus, 1767), is a polyphagous dictyopharid planthopper of Auchenorrhyncha commonly found throughout the Palaearctic. Despite abundant data on its distribution range and reports on its role in the epidemiology of plant-pathogenic phytoplasmas (Flavescence dore, FD-C), literature regarding the biology and host plants of this species is scarce. Therefore, the aims of our study were to investigate the seasonal occurrence, host plant associations, oviposition behaviour and immature stages of this widespread planthopper of economic importance. We performed a 3-year field study to observe the spatiotemporal distribution and feeding sources of D. europaea. The insects's reproductive strategy, nymphal molting and behaviour were observed under semi-field cage conditions. Measurement of the nymphal vertex length was used to determine the number of instars, and the combination of these data with body length, number of pronotal rows of sensory pits and body colour pattern enabled the discrimination of each instar. We provide data showing that D. europaea has five instars with one generation per year and that it overwinters in the egg stage. Furthermore, our study confirmed highly polyphagous feeding nature of D. europaea, for all instars and adults, as well as adult horizontal movement during the vegetation growing season to the temporarily preferred feeding plants where they aggregate during dry season. We found D. europaea adult aggregation in late summer on Clematis vitalba L. (Ranunculaceae), a reservoir plant of FD-C phytoplasma strain; however, this appears to be a consequence of forced migration due to drying of herbaceous vegetation rather than to a high preference of C. vitalba as a feeding plant. Detailed oviposition behaviour and a summary of the key discriminatory characteristics of the five instars are provided. Emphasis is placed on the economic importance of D. europaea because of its involvement in epidemiological cycles of phytoplasma-induced plant diseases.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations",
pages = "405-395",
number = "3",
volume = "106",
doi = "10.1017/S0007485316000067"
}

Krstić, O., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 106(3), 395-405.
https://doi.org/10.1017/S0007485316000067

Krstić O, Cvrković T, Mitrović M, Toševski I, Jović J. Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations. in Bulletin of Entomological Research. 2016;106(3):395-405.
doi:10.1017/S0007485316000067 .

Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Dictyophara europaea (Hemiptera: Fulgoromorpha: Dictyopharidae): description of immatures, biology and host plant associations" in Bulletin of Entomological Research, 106, no. 3 (2016):395-405,
https://doi.org/10.1017/S0007485316000067 . .

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Radonjić, Sanja
AU  - Hrnčić, Snježana
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/430
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards
EP  - 3
IS  - 1
SP  - 1
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Radonjić, Sanja and Hrnčić, Snježana and Krstić, Oliver and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards",
pages = "3-1",
number = "1",
volume = "66"
}

Kosovac, A., Radonjić, S., Hrnčić, S., Krstić, O., Toševski, I.,& Jović, J.. (2016). The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 1-3.

Kosovac A, Radonjić S, Hrnčić S, Krstić O, Toševski I, Jović J. The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards. in Mitteilungen Klosterneuburg. 2016;66(1):1-3..

Kosovac, Andrea, Radonjić, Sanja, Hrnčić, Snježana, Krstić, Oliver, Toševski, Ivo, Jović, Jelena, "The role of vitex agnus-castus and associated hyalesthes obsoletus in the epidemiology of bois noir in me-diterranean vineyards" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):1-3.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/431
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data
EP  - 11
IS  - 1
SP  - 9
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data",
pages = "11-9",
number = "1",
volume = "66"
}

Kosovac, A., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Toševski, I.,& Jović, J.. (2016). Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 9-11.

Kosovac A, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Toševski I, Jović J. Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data. in Mitteilungen Klosterneuburg. 2016;66(1):9-11..

Kosovac, Andrea, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Toševski, Ivo, Jović, Jelena, "Elucidation of 'candidatus phytoplasma solani' epidemiology through trac(k)ing transmission pathways using field, experimental and molecular data" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):9-11.

TY  - JOUR
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Kosovac, Andrea
AU  - Trivellone, Valeria
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/432
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia
EP  - 18
IS  - 1
SP  - 17
VL  - 66
ER  - 

@article{
author = "Mitrović, Milana and Jakovljević, Miljana and Jović, Jelena and Krstić, Oliver and Kosovac, Andrea and Trivellone, Valeria and Toševski, Ivo and Cvrković, Tatjana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia",
pages = "18-17",
number = "1",
volume = "66"
}

Mitrović, M., Jakovljević, M., Jović, J., Krstić, O., Kosovac, A., Trivellone, V., Toševski, I.,& Cvrković, T.. (2016). Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 17-18.

Mitrović M, Jakovljević M, Jović J, Krstić O, Kosovac A, Trivellone V, Toševski I, Cvrković T. Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia. in Mitteilungen Klosterneuburg. 2016;66(1):17-18..

Mitrović, Milana, Jakovljević, Miljana, Jović, Jelena, Krstić, Oliver, Kosovac, Andrea, Trivellone, Valeria, Toševski, Ivo, Cvrković, Tatjana, "Epidemiology of 'candidatus phytoplasma solani' associated with potato stolbur disease in Serbia" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):17-18.

TY  - JOUR
AU  - Trivellone, Valeria
AU  - Jermini, Mauro
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Kosovac, Andrea
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Mitrović, Milana
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/433
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland
EP  - 23
IS  - 1
SP  - 22
VL  - 66
ER  - 

@article{
author = "Trivellone, Valeria and Jermini, Mauro and Jović, Jelena and Cvrković, Tatjana and Jakovljević, Miljana and Kosovac, Andrea and Krstić, Oliver and Toševski, Ivo and Mitrović, Milana",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland",
pages = "23-22",
number = "1",
volume = "66"
}

Trivellone, V., Jermini, M., Jović, J., Cvrković, T., Jakovljević, M., Kosovac, A., Krstić, O., Toševski, I.,& Mitrović, M.. (2016). Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 22-23.

Trivellone V, Jermini M, Jović J, Cvrković T, Jakovljević M, Kosovac A, Krstić O, Toševski I, Mitrović M. Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland. in Mitteilungen Klosterneuburg. 2016;66(1):22-23..

Trivellone, Valeria, Jermini, Mauro, Jović, Jelena, Cvrković, Tatjana, Jakovljević, Miljana, Kosovac, Andrea, Krstić, Oliver, Toševski, Ivo, Mitrović, Milana, "Prevalence of stolbur phytoplasma in leafhoppers and planthoppers collected in vineyard, corn and potato fields and their surroundings in Switzerland" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):22-23.

TY  - JOUR
AU  - Kosovac, Andrea
AU  - Johannesen, Jes
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Jović, Jelena
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/434
PB  - Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg
T2  - Mitteilungen Klosterneuburg
T1  - Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe
EP  - 25
IS  - 1
SP  - 24
VL  - 66
ER  - 

@article{
author = "Kosovac, Andrea and Johannesen, Jes and Krstić, Oliver and Mitrović, Milana and Cvrković, Tatjana and Toševski, Ivo and Jović, Jelena",
year = "2016",
publisher = "Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg",
journal = "Mitteilungen Klosterneuburg",
title = "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe",
pages = "25-24",
number = "1",
volume = "66"
}

Kosovac, A., Johannesen, J., Krstić, O., Mitrović, M., Cvrković, T., Toševski, I.,& Jović, J.. (2016). Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg
Hoehere Bundeslehranstalt Und Bundesamt Fuer Wein- Und Obstbau, Klosterneuburg., 66(1), 24-25.

Kosovac A, Johannesen J, Krstić O, Mitrović M, Cvrković T, Toševski I, Jović J. Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe. in Mitteilungen Klosterneuburg. 2016;66(1):24-25..

Kosovac, Andrea, Johannesen, Jes, Krstić, Oliver, Mitrović, Milana, Cvrković, Tatjana, Toševski, Ivo, Jović, Jelena, "Is hyalesthes obsoletus a species complex undergoing cryptic speciation? More evidence of host-associated genetic differentiation in Southeast Europe" in Mitteilungen Klosterneuburg, 66, no. 1 (2016):24-25.