TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Zečević, Katarina
AU  - Stanisavljević, Rade
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/714
AB  - The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.
PB  - British Society for Plant Pathology
T2  - PLANT PATHOLOGY
T1  - Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans
EP  - 299
IS  - 2
SP  - 290
VL  - 72
DO  - 10.1111/ppa.13658
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Milovanović, Predrag and Stanković, Slaviša and Zečević, Katarina and Stanisavljević, Rade and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.",
publisher = "British Society for Plant Pathology",
journal = "PLANT PATHOLOGY",
title = "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans",
pages = "299-290",
number = "2",
volume = "72",
doi = "10.1111/ppa.13658"
}

Iličić, R., Jelušić, A., Milovanović, P., Stanković, S., Zečević, K., Stanisavljević, R.,& Popović Milovanović, T.. (2023). Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in PLANT PATHOLOGY
British Society for Plant Pathology., 72(2), 290-299.
https://doi.org/10.1111/ppa.13658

Iličić R, Jelušić A, Milovanović P, Stanković S, Zečević K, Stanisavljević R, Popović Milovanović T. Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in PLANT PATHOLOGY. 2023;72(2):290-299.
doi:10.1111/ppa.13658 .

Iličić, Renata, Jelušić, Aleksandra, Milovanović, Predrag, Stanković, Slaviša, Zečević, Katarina, Stanisavljević, Rade, Popović Milovanović, Tatjana, "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans" in PLANT PATHOLOGY, 72, no. 2 (2023):290-299,
https://doi.org/10.1111/ppa.13658 . .

TY  - CONF
AU  - Iličić, Renata
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Vlajić, Slobodan
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1342
AB  - Bacterial canker caused by Pseudomonas syringae pathovars is one of the most serious cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly based from imported planting material are being raised every year. In general, bacterial canker is mostly distributed in young sweet cherry plantations worldwide and in Serbia also. During spring, in May 2018 and 2019, occurrence of bacterial canker symptoms was observed on a newly planted sweet cherry plantations on two localities, Zitorada and Karavukovo. Therefore the aim of this work was to determine causal agent of the disease using conventional and molecular tools. 
Typical P. syringae colonies were isolated on Nutrient Sucrose Agar from all collected sweet cher­ry samples with expressed bacterial canker or leaf spot symptoms. All isolates were Gram negative, catalase positive, produced fluorescent pigment on King B medium under UV light and showed oxidative metabolism of glucose. Results of the LOPAT test (+---+) determined isolates to belong to fluorescent Pseudomonas Group la, while in G•A•TTa· showed characteristics described for pv. syringae. Pathogenicity was confirmed on immature fruits of sweet and sour cherry where all isolates caused typical symptoms in form of black, sunken lesions, characteristics for pv. syringae. Selected bacterial isolates were further identified by DNA sequencing using gyrB housekeeping gene. All tested isolates were identified as P. syringae pv. syringae using NCBI BLAST. Isolate REOS, isolated from sweet cherry stem (Karavukovo) showed 9g.51% homology with P. syringae pv. syringae strain CFBP421 S (sweet cherry, France). Isolates RE3 and RE4 (Zitorada) originated from branch and leaf, respectively, showed 99.19% homology with P. syringae pv. syringae strain Pss9097 (sweet cherry, UK) and CFBP2118 (sweet cherry, France). Phylogenetic analysis grouped isolates from 2itorada (RE3 and RE4) in one tree cluster, separate from the isolate REOS, showing presence of two genetically diverse groups of P. syringae pv. syringae isolates, obtained from two geographically distinct localities in Serbia. 
Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check needs to be in­cluded as obligatory measure when nursery materials is used from import.
PB  - Društvo za zaštitu bilja
C3  - VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia
T1  - NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS
SP  - 185
ER  - 

@conference{
author = "Iličić, Renata and Popović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Vlajić, Slobodan",
year = "2019",
abstract = "Bacterial canker caused by Pseudomonas syringae pathovars is one of the most serious cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly based from imported planting material are being raised every year. In general, bacterial canker is mostly distributed in young sweet cherry plantations worldwide and in Serbia also. During spring, in May 2018 and 2019, occurrence of bacterial canker symptoms was observed on a newly planted sweet cherry plantations on two localities, Zitorada and Karavukovo. Therefore the aim of this work was to determine causal agent of the disease using conventional and molecular tools. 
Typical P. syringae colonies were isolated on Nutrient Sucrose Agar from all collected sweet cher­ry samples with expressed bacterial canker or leaf spot symptoms. All isolates were Gram negative, catalase positive, produced fluorescent pigment on King B medium under UV light and showed oxidative metabolism of glucose. Results of the LOPAT test (+---+) determined isolates to belong to fluorescent Pseudomonas Group la, while in G•A•TTa· showed characteristics described for pv. syringae. Pathogenicity was confirmed on immature fruits of sweet and sour cherry where all isolates caused typical symptoms in form of black, sunken lesions, characteristics for pv. syringae. Selected bacterial isolates were further identified by DNA sequencing using gyrB housekeeping gene. All tested isolates were identified as P. syringae pv. syringae using NCBI BLAST. Isolate REOS, isolated from sweet cherry stem (Karavukovo) showed 9g.51% homology with P. syringae pv. syringae strain CFBP421 S (sweet cherry, France). Isolates RE3 and RE4 (Zitorada) originated from branch and leaf, respectively, showed 99.19% homology with P. syringae pv. syringae strain Pss9097 (sweet cherry, UK) and CFBP2118 (sweet cherry, France). Phylogenetic analysis grouped isolates from 2itorada (RE3 and RE4) in one tree cluster, separate from the isolate REOS, showing presence of two genetically diverse groups of P. syringae pv. syringae isolates, obtained from two geographically distinct localities in Serbia. 
Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check needs to be in­cluded as obligatory measure when nursery materials is used from import.",
publisher = "Društvo za zaštitu bilja",
journal = "VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia",
title = "NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS",
pages = "185"
}

Iličić, R., Popović, T., Jelušić, A., Marković, S.,& Vlajić, S.. (2019). NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia
Društvo za zaštitu bilja., 185.

Iličić R, Popović T, Jelušić A, Marković S, Vlajić S. NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia. 2019;:185..

Iličić, Renata, Popović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Vlajić, Slobodan, "NEW RECORDS OF Pseudomonas syringae IN YOUNG SWEET CHERRY PLANTATIONS" in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia (2019):185.