TY  - CONF
AU  - Vučurović, Ivan
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Blagojević, Jovana
AU  - Đukanović, Jelena
AU  - Zečević, Katarina
AU  - Stanković, Ivana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1314
AB  - Garlic (Allium sativum L.) has been cultivated as a vegetable for over 5000 years. In Serbia, garlic farming occurs across various geographic regions, with the main cultivation area being the Vojvodina province. Viruses pose a significant threat to garlic crops, especially due to ability to spread easily through vegetative propagation. Garlic plants are susceptible to infection by viruses from three main families: Potyviridae (genus Potyvirus), Betaflexiviridae (genus Carlavirus), and Alphaflexiviridae (genus Allexivirus). Among them, garlic common latent virus (GarCLV) stands out as one of the most prevalent viruses affecting garlic. Despite often showing no visible symptoms, GarCLV plays a crucial role as a synergistic agent, particularly when combined with other plant viruses, notably potyviruses. The first report of GarCLV infecting garlic in Serbia was reported in 2011, but the extent of its incidence and genetic diversity remained unclear. In this study, 130 garlic plants were collected and tested for the presence of GarCLV by double-antibody sandwich (DAS)-ELISA using commercial diagnostic kit (Bioreba, AG, Reinach, Switzerland). After detection of GarCLV presence through ELISA, further confirmation, and molecular characterization two selected samples was carried out using RT-PCR with GarCLV-specific primer pair, 1-GCLV and 2-GCLV, which amplified a 960-bp fragment of the entire coat protein (CP) gene. The amplified products were purified, sequenced in both directions using the same primer pair, and deposited in GenBank (Acc. No. KP208802 and MK814828). The serological results showed a high prevalence of GarCLV in garlic crops in Serbia, and the phylogenetic studies showed that GarCLV isolates exhibited significant genetic similarity and were grouped in the same cluster. Considering that GarCLV is mainly transmitted through propagation material and the risk of a synergistic effect due to mixed infections with other garlic viruses, special measures should be taken to control impact of this virus on garlic production.
PB  - University of Banja Luka, Faculty of Agriculture
C3  - XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina
T1  - Incidence and genetic diversity of garlic common latent virus infecting garlic in Serbia
SP  - 89
ER  - 

@conference{
author = "Vučurović, Ivan and Vučurović, Ana and Ristić, Danijela and Blagojević, Jovana and Đukanović, Jelena and Zečević, Katarina and Stanković, Ivana",
year = "2024",
abstract = "Garlic (Allium sativum L.) has been cultivated as a vegetable for over 5000 years. In Serbia, garlic farming occurs across various geographic regions, with the main cultivation area being the Vojvodina province. Viruses pose a significant threat to garlic crops, especially due to ability to spread easily through vegetative propagation. Garlic plants are susceptible to infection by viruses from three main families: Potyviridae (genus Potyvirus), Betaflexiviridae (genus Carlavirus), and Alphaflexiviridae (genus Allexivirus). Among them, garlic common latent virus (GarCLV) stands out as one of the most prevalent viruses affecting garlic. Despite often showing no visible symptoms, GarCLV plays a crucial role as a synergistic agent, particularly when combined with other plant viruses, notably potyviruses. The first report of GarCLV infecting garlic in Serbia was reported in 2011, but the extent of its incidence and genetic diversity remained unclear. In this study, 130 garlic plants were collected and tested for the presence of GarCLV by double-antibody sandwich (DAS)-ELISA using commercial diagnostic kit (Bioreba, AG, Reinach, Switzerland). After detection of GarCLV presence through ELISA, further confirmation, and molecular characterization two selected samples was carried out using RT-PCR with GarCLV-specific primer pair, 1-GCLV and 2-GCLV, which amplified a 960-bp fragment of the entire coat protein (CP) gene. The amplified products were purified, sequenced in both directions using the same primer pair, and deposited in GenBank (Acc. No. KP208802 and MK814828). The serological results showed a high prevalence of GarCLV in garlic crops in Serbia, and the phylogenetic studies showed that GarCLV isolates exhibited significant genetic similarity and were grouped in the same cluster. Considering that GarCLV is mainly transmitted through propagation material and the risk of a synergistic effect due to mixed infections with other garlic viruses, special measures should be taken to control impact of this virus on garlic production.",
publisher = "University of Banja Luka, Faculty of Agriculture",
journal = "XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina",
title = "Incidence and genetic diversity of garlic common latent virus infecting garlic in Serbia",
pages = "89"
}

Vučurović, I., Vučurović, A., Ristić, D., Blagojević, J., Đukanović, J., Zečević, K.,& Stanković, I.. (2024). Incidence and genetic diversity of garlic common latent virus infecting garlic in Serbia. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina
University of Banja Luka, Faculty of Agriculture., 89.

Vučurović I, Vučurović A, Ristić D, Blagojević J, Đukanović J, Zečević K, Stanković I. Incidence and genetic diversity of garlic common latent virus infecting garlic in Serbia. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina. 2024;:89..

Vučurović, Ivan, Vučurović, Ana, Ristić, Danijela, Blagojević, Jovana, Đukanović, Jelena, Zečević, Katarina, Stanković, Ivana, "Incidence and genetic diversity of garlic common latent virus infecting garlic in Serbia" in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina (2024):89.

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Trkulja, Vojislav
AU  - Milovanović, Predrag
AU  - Zečević, Katarina
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1198
AB  - Veliki ekonomski značaj u gajenju brojnih biljnih vrsta, a posebno korenasto-krtolastih,
imaju bakterije iz rodova Pectobacterium i Dickeya, prvobitno opisane kao rod Erwinia. Ove
bakterije prouzrokuju bolest tzv. vlažnu trulež. Štete nastaju usled propadanja zaraženih
plodova, krtola i mesnatog korena. Iako ovi patogeni mogu da prouzrokuju velike štete tokom
proizvodnje, ipak najveće nastaju tokom skladištenja. Primenom metode sekvenciranja DNK
genoma, taksonomija rodova Pectobacterium i Dickeya je u prethodnoj deceniji značajno
izmenjena,usled čega je i pripadnost pojedinih, već opisanih sojeva vrstama iz ovih rodova
iznova determinisana. Takođe, ova metodologija doprinela je otkrivanju velikog broja novih
vrsta bakterija ovih rodova. Tako, rod Pectobacterium do danas broji 20 vrsta, a rod Dickeya
najmanje 10. S obzirom na učestale taksonomske promene, cilj ovog rada je bio da se izoluju i
identifikuju prouzrokovači vlažne truleži paprike i mrkve kako bi se utvrdio genetički
diverzitet novoizolovanih bakterijskih populacija.
Tokom septembra 2022. godine, na lokalitetu Rimski Šančevi prikupljeni su oboleli
uzorci plodova paprike sa simptomima vlažne truleži, a na lokalitetu Futog uzorci korena
mrkve. Simptomi su se javljali u vidu razmekšavanja i propadanja plodova, uz prisustvo
karakterističnog, neprijatnog mirisa. Izolacije su vršene sa prelaznih zona između obolelog i
zdravog tkiva, na podlogu sa saharozom i peptonom. Bakterijske kolonije beličasto krem boje,
glatke, blago ispupčene su se javljale 48 h nakon izolacije i inkubacije na 26 °C. Patogenost
odabranih izolata dokazana je na zrelim plodovima paprike, korenu mrkve, kao i krtolama
krompira. Analiza sekvenci više genskih lokusa (MLSA) vršena je primenom šest
konzerviranih gena dnaX, gapA, icdA, mdh, recA i rpoS. BLASTn analiza Nacionalnog centra za
biotehnološke informacije (NCBI) je pokazala da izolati poreklom sa paprike pripadaju
bakteriji Pectobacterium brasiliense, sa procentom identiteta od 98,96% (dnaX) do 100%
(gapA). Izolati poreklom sa mrkve su identifikovani kao Pectobacterium carotovorum, sa
procentom identiteta od 100%, na osnovu sekvenci svih šest analiziranih gena. Ove bakterije
su u Srbiji prethodno determinisane i opisane kao patogeni krompira, a P. carotovorum i kao
patogen kupusa.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini
SP  - 48
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Iličić, Renata and Jelušić, Aleksandra and Mitrović, Petar and Trkulja, Vojislav and Milovanović, Predrag and Zečević, Katarina",
year = "2023",
abstract = "Veliki ekonomski značaj u gajenju brojnih biljnih vrsta, a posebno korenasto-krtolastih,
imaju bakterije iz rodova Pectobacterium i Dickeya, prvobitno opisane kao rod Erwinia. Ove
bakterije prouzrokuju bolest tzv. vlažnu trulež. Štete nastaju usled propadanja zaraženih
plodova, krtola i mesnatog korena. Iako ovi patogeni mogu da prouzrokuju velike štete tokom
proizvodnje, ipak najveće nastaju tokom skladištenja. Primenom metode sekvenciranja DNK
genoma, taksonomija rodova Pectobacterium i Dickeya je u prethodnoj deceniji značajno
izmenjena,usled čega je i pripadnost pojedinih, već opisanih sojeva vrstama iz ovih rodova
iznova determinisana. Takođe, ova metodologija doprinela je otkrivanju velikog broja novih
vrsta bakterija ovih rodova. Tako, rod Pectobacterium do danas broji 20 vrsta, a rod Dickeya
najmanje 10. S obzirom na učestale taksonomske promene, cilj ovog rada je bio da se izoluju i
identifikuju prouzrokovači vlažne truleži paprike i mrkve kako bi se utvrdio genetički
diverzitet novoizolovanih bakterijskih populacija.
Tokom septembra 2022. godine, na lokalitetu Rimski Šančevi prikupljeni su oboleli
uzorci plodova paprike sa simptomima vlažne truleži, a na lokalitetu Futog uzorci korena
mrkve. Simptomi su se javljali u vidu razmekšavanja i propadanja plodova, uz prisustvo
karakterističnog, neprijatnog mirisa. Izolacije su vršene sa prelaznih zona između obolelog i
zdravog tkiva, na podlogu sa saharozom i peptonom. Bakterijske kolonije beličasto krem boje,
glatke, blago ispupčene su se javljale 48 h nakon izolacije i inkubacije na 26 °C. Patogenost
odabranih izolata dokazana je na zrelim plodovima paprike, korenu mrkve, kao i krtolama
krompira. Analiza sekvenci više genskih lokusa (MLSA) vršena je primenom šest
konzerviranih gena dnaX, gapA, icdA, mdh, recA i rpoS. BLASTn analiza Nacionalnog centra za
biotehnološke informacije (NCBI) je pokazala da izolati poreklom sa paprike pripadaju
bakteriji Pectobacterium brasiliense, sa procentom identiteta od 98,96% (dnaX) do 100%
(gapA). Izolati poreklom sa mrkve su identifikovani kao Pectobacterium carotovorum, sa
procentom identiteta od 100%, na osnovu sekvenci svih šest analiziranih gena. Ove bakterije
su u Srbiji prethodno determinisane i opisane kao patogeni krompira, a P. carotovorum i kao
patogen kupusa.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini",
pages = "48"
}

Popović Milovanović, T., Iličić, R., Jelušić, A., Mitrović, P., Trkulja, V., Milovanović, P.,& Zečević, K.. (2023). Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 48.

Popović Milovanović T, Iličić R, Jelušić A, Mitrović P, Trkulja V, Milovanović P, Zečević K. Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:48..

Popović Milovanović, Tatjana, Iličić, Renata, Jelušić, Aleksandra, Mitrović, Petar, Trkulja, Vojislav, Milovanović, Predrag, Zečević, Katarina, "Proučavanje prouzrokovača vlažne truleži paprike i mrkve u Vojvodini" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):48.

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Zečević, Katarina
AU  - Stanisavljević, Rade
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/714
AB  - The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.
PB  - British Society for Plant Pathology
T2  - PLANT PATHOLOGY
T1  - Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans
EP  - 299
IS  - 2
SP  - 290
VL  - 72
DO  - 10.1111/ppa.13658
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Milovanović, Predrag and Stanković, Slaviša and Zečević, Katarina and Stanisavljević, Rade and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The present study provides a new insight into the existing Xanthomonas arboricola pv. pruni (Xap) bacterial population originating from peach and apricot in two Western Balkans countries (Serbia and Montenegro). Multilocus sequence typing and analysis of the sequences of nine housekeeping genes revealed homology between the tested Xap strains as well as with the European population of this bacterium. The tested strains share the same haplotype (Haplotype I) with the Xap strains from Italy, France, Spain, United States, Australia and Brazil. The revealed single nucleotide change (G ↔ C) in the sequences of the gyrB1 gene differentiates Haplotype I from Haplotype II (Xap from South Korea, New Zealand, Argentina and Uruguay). The detached-leaf bioassay results confirmed differences in virulence between strains originating from peach and apricot towards Prunus armeniaca (apricot), indicating host specialization of the apricot strain towards this host. For the first time, immunity of P. fruticosa (European ground cherry) to Xap was established. According to the AUDPC, PCA and cluster analysis, other Prunus spp. were classified as having low susceptibility (P. mahaleb, P. cerasus and P. avium), as susceptible (P. domestica) and as highly susceptible (P. persica, P. dulcis, P. cerasifera and P. spinosa). Xap strains were also found to be susceptible to 10 tested antibiotics. This study provides valuable knowledge on the Xap population from stone fruit grown in the Western Balkans region as well as the source of immunity, which could serve as a starting point for breeding Prunus cultivars and could be used as the main control strategy.",
publisher = "British Society for Plant Pathology",
journal = "PLANT PATHOLOGY",
title = "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans",
pages = "299-290",
number = "2",
volume = "72",
doi = "10.1111/ppa.13658"
}

Iličić, R., Jelušić, A., Milovanović, P., Stanković, S., Zečević, K., Stanisavljević, R.,& Popović Milovanović, T.. (2023). Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in PLANT PATHOLOGY
British Society for Plant Pathology., 72(2), 290-299.
https://doi.org/10.1111/ppa.13658

Iličić R, Jelušić A, Milovanović P, Stanković S, Zečević K, Stanisavljević R, Popović Milovanović T. Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans. in PLANT PATHOLOGY. 2023;72(2):290-299.
doi:10.1111/ppa.13658 .

Iličić, Renata, Jelušić, Aleksandra, Milovanović, Predrag, Stanković, Slaviša, Zečević, Katarina, Stanisavljević, Rade, Popović Milovanović, Tatjana, "Characterization of Xanthomonas arboricola pv. pruni from Prunus spp. orchards in Western Balkans" in PLANT PATHOLOGY, 72, no. 2 (2023):290-299,
https://doi.org/10.1111/ppa.13658 . .