TY  - CONF
AU  - Živković, Ivana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Trkulja, Nenad
AU  - Adžić, Slađan
AU  - Damnjanović, Jelena
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1200
AB  - Primena sintetičkih pesticida u zaštiti bilja se poslednjih godina sve više zamenjuje
ekološki prihvatljivim i zdravstveno bezbednim rešenjima, što podrazumeva i primenu
mikroorganizama kao biokontrolnih agenasa u suzbijanju biljnih bolesti i štetočina. Bacillus
velezensis je gram-pozitivna bakterija koju je moguće izolovati iz različitih niša, uglavnom
zemljišta, vode i korena biljaka. Kao rizobakterijski organizam poznat je po sposobnosti da
podstiče imunitet biljke u uslovima stresa, biotičke ili abiotičke prirode. Cilj ovog rada je
ispitivanje potencijala B. velezensis soja P64 poreklom sa semena paprike (sorta Župska
Rana) za suzbijanje fitopatogene bakterije Xanthomonas euvesicatoria, prouzrokovača
bakteriozne pegavosti paprike, u in vivo uslovima.
Eksperiment je postavljen na sorti paprike Župska rana gajenoj u poljskim uslovima
(lokalitet Čenta) na kojoj su izvedena dva tretmana, prvi u fenofazi razvoja listova, a drugi
tokom cvetanja. Izolat X22 X. euvesicatoria i B. velezensis soj P64 su gajeni na YDC i LB
podlozi, redom, i pripremljene su bakterijske suspenzije koncentracije 107 CFU mL-1 za
fitopatogena, odnosno 108 CFU mL-1 za antagonistu. Tretmani su podrazumevali folijarnu
primenu soja B. velezensis 48 h pre (preventivni tretman) i 48 h nakon inokulacije paprike
(prskanjem) sa X. euvesicatoria izolatom X22 (kurativni tretman). Eksperimentalna parcela je
redovno zalivana, a biljke su svakodnevno prskane vodom kako bi se obezbedili uslovi
vlažnosti koji pogoduju ostvarivanju i širenju infekcije. Ocena intenziteta zaraze je vršena
ukupno 6 puta, na svakih sedam dana od primene tretmana, korišćenjem skale 0-4 (0=bez
simptoma, 1=10% lezija na površini lista, 2=25%, 3=50-80% i 4=>80% površine lista sa
lezijama). Efikasnost primenjenih tretmana izračunata je preko Abbott-ove formule.
Efikasnost preventivnih tretmana sa B. velezensis sojem P64 se kretala od 74,43%, 7
dana posle tretmana (DPT), do 98,09% 49 DPT, uz intenzitet zaraze 4,72% prilikom prve
ocene, a 0,61% tokom poslednje ocene. Efikasnost kurativnih tretmana se kretala od 90,41%
7 DPT, do 97,43% 49 DPT, a intenzitet zaraze je bio 1,77% (7 DPT) odnosno 0,82% (49 DPT).
U kontroli je zabeležen intenzitet zaraze od 18,46%, 24,39%, 26,37%, 31,06%, 27,58%,
28,11% i 31,92% ocenjen 7, 14, 21, 28, 35, 42 i 49 DPT, redom. Dobijeni rezultati ukazuju na
visok potencijal B. velezensis soja P64 u kontroli bakteriozne pegavosti paprike.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Potencijal Bacillus velezensis soja P64 poreklom sa semena paprike u suzbijanju Xanthomonas euvesicatoria
SP  - 81
ER  - 

@conference{
author = "Živković, Ivana and Jelušić, Aleksandra and Iličić, Renata and Trkulja, Nenad and Adžić, Slađan and Damnjanović, Jelena and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Primena sintetičkih pesticida u zaštiti bilja se poslednjih godina sve više zamenjuje
ekološki prihvatljivim i zdravstveno bezbednim rešenjima, što podrazumeva i primenu
mikroorganizama kao biokontrolnih agenasa u suzbijanju biljnih bolesti i štetočina. Bacillus
velezensis je gram-pozitivna bakterija koju je moguće izolovati iz različitih niša, uglavnom
zemljišta, vode i korena biljaka. Kao rizobakterijski organizam poznat je po sposobnosti da
podstiče imunitet biljke u uslovima stresa, biotičke ili abiotičke prirode. Cilj ovog rada je
ispitivanje potencijala B. velezensis soja P64 poreklom sa semena paprike (sorta Župska
Rana) za suzbijanje fitopatogene bakterije Xanthomonas euvesicatoria, prouzrokovača
bakteriozne pegavosti paprike, u in vivo uslovima.
Eksperiment je postavljen na sorti paprike Župska rana gajenoj u poljskim uslovima
(lokalitet Čenta) na kojoj su izvedena dva tretmana, prvi u fenofazi razvoja listova, a drugi
tokom cvetanja. Izolat X22 X. euvesicatoria i B. velezensis soj P64 su gajeni na YDC i LB
podlozi, redom, i pripremljene su bakterijske suspenzije koncentracije 107 CFU mL-1 za
fitopatogena, odnosno 108 CFU mL-1 za antagonistu. Tretmani su podrazumevali folijarnu
primenu soja B. velezensis 48 h pre (preventivni tretman) i 48 h nakon inokulacije paprike
(prskanjem) sa X. euvesicatoria izolatom X22 (kurativni tretman). Eksperimentalna parcela je
redovno zalivana, a biljke su svakodnevno prskane vodom kako bi se obezbedili uslovi
vlažnosti koji pogoduju ostvarivanju i širenju infekcije. Ocena intenziteta zaraze je vršena
ukupno 6 puta, na svakih sedam dana od primene tretmana, korišćenjem skale 0-4 (0=bez
simptoma, 1=10% lezija na površini lista, 2=25%, 3=50-80% i 4=>80% površine lista sa
lezijama). Efikasnost primenjenih tretmana izračunata je preko Abbott-ove formule.
Efikasnost preventivnih tretmana sa B. velezensis sojem P64 se kretala od 74,43%, 7
dana posle tretmana (DPT), do 98,09% 49 DPT, uz intenzitet zaraze 4,72% prilikom prve
ocene, a 0,61% tokom poslednje ocene. Efikasnost kurativnih tretmana se kretala od 90,41%
7 DPT, do 97,43% 49 DPT, a intenzitet zaraze je bio 1,77% (7 DPT) odnosno 0,82% (49 DPT).
U kontroli je zabeležen intenzitet zaraze od 18,46%, 24,39%, 26,37%, 31,06%, 27,58%,
28,11% i 31,92% ocenjen 7, 14, 21, 28, 35, 42 i 49 DPT, redom. Dobijeni rezultati ukazuju na
visok potencijal B. velezensis soja P64 u kontroli bakteriozne pegavosti paprike.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Potencijal Bacillus velezensis soja P64 poreklom sa semena paprike u suzbijanju Xanthomonas euvesicatoria",
pages = "81"
}

Živković, I., Jelušić, A., Iličić, R., Trkulja, N., Adžić, S., Damnjanović, J.,& Popović Milovanović, T.. (2023). Potencijal Bacillus velezensis soja P64 poreklom sa semena paprike u suzbijanju Xanthomonas euvesicatoria. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 81.

Živković I, Jelušić A, Iličić R, Trkulja N, Adžić S, Damnjanović J, Popović Milovanović T. Potencijal Bacillus velezensis soja P64 poreklom sa semena paprike u suzbijanju Xanthomonas euvesicatoria. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:81..

Živković, Ivana, Jelušić, Aleksandra, Iličić, Renata, Trkulja, Nenad, Adžić, Slađan, Damnjanović, Jelena, Popović Milovanović, Tatjana, "Potencijal Bacillus velezensis soja P64 poreklom sa semena paprike u suzbijanju Xanthomonas euvesicatoria" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):81.

TY  - JOUR
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/655
AB  - Xanthomonas arboricola pv. pruni (Xap) causes bacterial spot of leaves and fruits, defoliation, fruit dropping, and twig, branch, or trunk cankers in most cultivated and ornamental Prunus species. The bacterium is listed as a European and Mediterranean Plant Protection Organization (EPPO) A2 quarantine pathogen. Xap was first detected in 2019 on peach (Prunus persica L., unknown cultivar) leaves in a 13-year-old orchard located in Irig (GPS: 45°6′10.538″ N, 19°54′8.04″ E), with a disease incidence of 10 to 20%. Thereafter, Xap was detected in 2020 on apricot (Prunus armeniaca L., cultivars NS4, NS Rodna, and Roxana) leaves and fruits in a 5-year-old orchard located in Bešenovo (GPS: 45°04′59.0″ N, 19°41′23.0″ E), with disease incidence of 30 to 50%. Symptoms on leaves appeared along leaf midribs or margins in the form of brown to black spots, with a pale green to yellow halo, evident on both leaf surfaces. The diseased area on leaves dropped out, giving a shot-hole appearance; leaves turned yellow and dropped prematurely. Bacterial spots of apricot fruits appeared in the form of water-soaked or dark brown sunken lesions. Primarily, detection of Xap in collected samples was obtained using polymerase chain reaction (PCR) with Xap-specific primers XapY17-F/XapY17-R, after amplifying target fragments of 943 bp (Pothier et al. 2011). Xap reference strain NCPPB 3156 served as a positive control. Isolations performed on yeast extract–dextrose–calcium carbonate agar resulted in pale yellow, circular, raised and mucoid colonies after 3 days at 26°C. A total of 20 representative isolates were aerobic, gram negative, catalase positive, and oxidase and arginine dihydrolase negative; they induced a hypersensitivity reaction, reduced nitrates, hydrolyzed aesculin and gelatin but not starch, produced H2S, and did not produce indole (Schaad et al. 2001). NCBI BLASTn search of the ftsX (ABC transporter ATP-binding protein) sequences (Pothier et al. 2011) indicated 100% identity of Serbian isolates with Xap strains XAP HU2 (P. persica, MG049921) and XAP HU1 (P. armeniaca, KY039173) from Hungary. The nucleotide sequences of one isolate from peach (Xp219) and one from apricot (Xp320) were deposited in GenBank under accession numbers MT890969 and MT890970, respectively. Pathogenicity testing was performed on detached peach and apricot leaves for all 20 isolates and on leaves and shoots of potted 1-year-old plants of peach (cv. Vineyard Peach) and apricot (cv. NS Rodna) for three isolates from each host (Randhawa and Civerolo 1985; Suesada et al. 2013). Sterile distilled water and reference strain NCPPB 3156 were used as negative and positive controls. On detached leaves, all isolates caused typical water-soaked spots 3 days after inoculation (DAI), and at 10 DAI spots became brown and necrotic. The same symptoms appeared on the leaves of potted plants. On peach and apricot shoots water-soaked, slightly reddish lesions emerged at inoculation sites 7 to 10 DAI, and at 20 DAI lesions become dark, circular to elliptical, and 3.5 to 4 and 2.5 to 3 cm in size for peach and apricot, respectively. Negative controls were symptomless. Reisolated bacteria were confirmed to be the same as the original using PCR (Pothier et al. 2011), fulfilling Koch’s postulates. This is the first report of Xap in Serbia, which has occurred with a limited distribution in the Fruška Gora region (Vojvodina). Only two orchards in Serbia have been detected with Xap so far. In the diseased peach orchard Xap was eradicated by uprooting trees. The apricot orchard is still under official control to limit disease spread. Appropriate cultivation practices, national inspection, and surveillance are in place to prevent further pathogen spread and establishment to new hosts and regions in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Occurrence of Bacterial Spot Caused by Xanthomonas arboricola pv. pruni on Peach and Apricot in Serbia
IS  - 3
SP  - 697
VL  - 105
DO  - 10.1094/PDIS-08-20-1817-PDN
ER  - 

@article{
author = "Iličić, Renata and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Xanthomonas arboricola pv. pruni (Xap) causes bacterial spot of leaves and fruits, defoliation, fruit dropping, and twig, branch, or trunk cankers in most cultivated and ornamental Prunus species. The bacterium is listed as a European and Mediterranean Plant Protection Organization (EPPO) A2 quarantine pathogen. Xap was first detected in 2019 on peach (Prunus persica L., unknown cultivar) leaves in a 13-year-old orchard located in Irig (GPS: 45°6′10.538″ N, 19°54′8.04″ E), with a disease incidence of 10 to 20%. Thereafter, Xap was detected in 2020 on apricot (Prunus armeniaca L., cultivars NS4, NS Rodna, and Roxana) leaves and fruits in a 5-year-old orchard located in Bešenovo (GPS: 45°04′59.0″ N, 19°41′23.0″ E), with disease incidence of 30 to 50%. Symptoms on leaves appeared along leaf midribs or margins in the form of brown to black spots, with a pale green to yellow halo, evident on both leaf surfaces. The diseased area on leaves dropped out, giving a shot-hole appearance; leaves turned yellow and dropped prematurely. Bacterial spots of apricot fruits appeared in the form of water-soaked or dark brown sunken lesions. Primarily, detection of Xap in collected samples was obtained using polymerase chain reaction (PCR) with Xap-specific primers XapY17-F/XapY17-R, after amplifying target fragments of 943 bp (Pothier et al. 2011). Xap reference strain NCPPB 3156 served as a positive control. Isolations performed on yeast extract–dextrose–calcium carbonate agar resulted in pale yellow, circular, raised and mucoid colonies after 3 days at 26°C. A total of 20 representative isolates were aerobic, gram negative, catalase positive, and oxidase and arginine dihydrolase negative; they induced a hypersensitivity reaction, reduced nitrates, hydrolyzed aesculin and gelatin but not starch, produced H2S, and did not produce indole (Schaad et al. 2001). NCBI BLASTn search of the ftsX (ABC transporter ATP-binding protein) sequences (Pothier et al. 2011) indicated 100% identity of Serbian isolates with Xap strains XAP HU2 (P. persica, MG049921) and XAP HU1 (P. armeniaca, KY039173) from Hungary. The nucleotide sequences of one isolate from peach (Xp219) and one from apricot (Xp320) were deposited in GenBank under accession numbers MT890969 and MT890970, respectively. Pathogenicity testing was performed on detached peach and apricot leaves for all 20 isolates and on leaves and shoots of potted 1-year-old plants of peach (cv. Vineyard Peach) and apricot (cv. NS Rodna) for three isolates from each host (Randhawa and Civerolo 1985; Suesada et al. 2013). Sterile distilled water and reference strain NCPPB 3156 were used as negative and positive controls. On detached leaves, all isolates caused typical water-soaked spots 3 days after inoculation (DAI), and at 10 DAI spots became brown and necrotic. The same symptoms appeared on the leaves of potted plants. On peach and apricot shoots water-soaked, slightly reddish lesions emerged at inoculation sites 7 to 10 DAI, and at 20 DAI lesions become dark, circular to elliptical, and 3.5 to 4 and 2.5 to 3 cm in size for peach and apricot, respectively. Negative controls were symptomless. Reisolated bacteria were confirmed to be the same as the original using PCR (Pothier et al. 2011), fulfilling Koch’s postulates. This is the first report of Xap in Serbia, which has occurred with a limited distribution in the Fruška Gora region (Vojvodina). Only two orchards in Serbia have been detected with Xap so far. In the diseased peach orchard Xap was eradicated by uprooting trees. The apricot orchard is still under official control to limit disease spread. Appropriate cultivation practices, national inspection, and surveillance are in place to prevent further pathogen spread and establishment to new hosts and regions in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Occurrence of Bacterial Spot Caused by Xanthomonas arboricola pv. pruni on Peach and Apricot in Serbia",
number = "3",
pages = "697",
volume = "105",
doi = "10.1094/PDIS-08-20-1817-PDN"
}

Iličić, R.,& Popović Milovanović, T.. (2021). Occurrence of Bacterial Spot Caused by Xanthomonas arboricola pv. pruni on Peach and Apricot in Serbia. in Plant Disease
American Phytopathological Society., 105(3), 697.
https://doi.org/10.1094/PDIS-08-20-1817-PDN

Iličić R, Popović Milovanović T. Occurrence of Bacterial Spot Caused by Xanthomonas arboricola pv. pruni on Peach and Apricot in Serbia. in Plant Disease. 2021;105(3):697.
doi:10.1094/PDIS-08-20-1817-PDN .

Iličić, Renata, Popović Milovanović, Tatjana, "Occurrence of Bacterial Spot Caused by Xanthomonas arboricola pv. pruni on Peach and Apricot in Serbia" in Plant Disease, 105, no. 3 (2021):697,
https://doi.org/10.1094/PDIS-08-20-1817-PDN . .