TY  - CONF
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1251
AB  - Neoaliturus fenestratus (Herrich-Schäffer 1834) is a polyphagous leafhopper widespread throughout
Europe, particularly in ruderal habitats, i.e., abandoned fields, vineyards, and sparsely vegetated
meadows, commonly associated with different plants of the family Asteraceae (Nickel 2003).
Neoaliturus fenestratus is confirmed to transmit the safflower phyllody associated with phytoplasmas
from the 16SrI-B subgroup (Raccah & Klein 1982), Picris hieracioides bushy stunt caused by
phytoplasma from the 16SrII-E subgroup (Mitrović et al. 2012), lettuce phyllody and wild lettuce
phyllody associated with phytoplasmas belonging to the 16SrIX group (Salehi et al. 2007), and chicory
phyllody phytoplasma of the 16SrIX-C subgroup (Ermacora et al. 2013). Furthermore, Mitrović et al.
(2019) experimentally confirmed its vector role in transmitting 'Ca. P. solani' of the 16SrXII-A
subgroup to lettuce and carrot. In this study, N. fenestratus was investigated for its capacity to transmit
the 'Ca. P. solani' to grapevine (Vitis vinifera) and native plants commonly found in and/or around
vineyards in Serbia (Convolvulus arvensis, Calendula officinalis, Chenopodium album, and P.
hieracioides).
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - New insights on the ‘Ca. Phytoplasma solani’ transmission by Neoaliturus fenestratus (Cicadellidae: Deltocephalinae)
EP  - 55
SP  - 54
ER  - 

@conference{
author = "Mitrović, Milana and Jakovljević, Miljana and Marinković, Slavica and Toševski, Ivo and Jović, Jelena and Krstić, Oliver and Cvrković, Tatjana",
year = "2024",
abstract = "Neoaliturus fenestratus (Herrich-Schäffer 1834) is a polyphagous leafhopper widespread throughout
Europe, particularly in ruderal habitats, i.e., abandoned fields, vineyards, and sparsely vegetated
meadows, commonly associated with different plants of the family Asteraceae (Nickel 2003).
Neoaliturus fenestratus is confirmed to transmit the safflower phyllody associated with phytoplasmas
from the 16SrI-B subgroup (Raccah & Klein 1982), Picris hieracioides bushy stunt caused by
phytoplasma from the 16SrII-E subgroup (Mitrović et al. 2012), lettuce phyllody and wild lettuce
phyllody associated with phytoplasmas belonging to the 16SrIX group (Salehi et al. 2007), and chicory
phyllody phytoplasma of the 16SrIX-C subgroup (Ermacora et al. 2013). Furthermore, Mitrović et al.
(2019) experimentally confirmed its vector role in transmitting 'Ca. P. solani' of the 16SrXII-A
subgroup to lettuce and carrot. In this study, N. fenestratus was investigated for its capacity to transmit
the 'Ca. P. solani' to grapevine (Vitis vinifera) and native plants commonly found in and/or around
vineyards in Serbia (Convolvulus arvensis, Calendula officinalis, Chenopodium album, and P.
hieracioides).",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "New insights on the ‘Ca. Phytoplasma solani’ transmission by Neoaliturus fenestratus (Cicadellidae: Deltocephalinae)",
pages = "55-54"
}

Mitrović, M., Jakovljević, M., Marinković, S., Toševski, I., Jović, J., Krstić, O.,& Cvrković, T.. (2024). New insights on the ‘Ca. Phytoplasma solani’ transmission by Neoaliturus fenestratus (Cicadellidae: Deltocephalinae). in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 54-55.

Mitrović M, Jakovljević M, Marinković S, Toševski I, Jović J, Krstić O, Cvrković T. New insights on the ‘Ca. Phytoplasma solani’ transmission by Neoaliturus fenestratus (Cicadellidae: Deltocephalinae). in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:54-55..

Mitrović, Milana, Jakovljević, Miljana, Marinković, Slavica, Toševski, Ivo, Jović, Jelena, Krstić, Oliver, Cvrković, Tatjana, "New insights on the ‘Ca. Phytoplasma solani’ transmission by Neoaliturus fenestratus (Cicadellidae: Deltocephalinae)" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):54-55.

TY  - CONF
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1256
AB  - The planthopper Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) is a major vector and
driver of 'Ca. Phytoplasma solani' epidemiology (Maixner, 1994; Jović & Toševski, 2023) and
associated diseases of cultivated plants, including the grapevine-Bois noir (BN) pathosystem. Because
of its strong association with its host plants as a subterranean nymph and short life span as an adult
(Cargnus et al., 2012), the epidemiological cycle is host plant-specific and is determined by the vector's
host range and the pathogen's reservoir range (Imo et al., 2013; Maixner et al., 2014). Although H.
obsoletus is commonly viewed as a polyphagous insect, there is plenty of proof of host plant adaptation
in its populations, which are referred to as host races, biotypes, or cryptic species (Imo et al., 2013;
Maixner et al., 2014; Kosovac et al., 2016; 2018; 2019). The first described and most prevalent
epidemiological cycles linked with distinct strains of 'Ca. P. solani' are driven by nettle and bindweed
as pathogen reservoir plants and H. obsoletus host plants (Langer & Maixner, 2004). The genetic
distinction between the two pathosystems was initially identified on the tuf gene and then verified by
secY, stamp, and vmp1 gene typing (Langer & Maixner, 2004; Johannesen et al., 2012; Aryan et al.,
2014). In Serbia and the Balkans, nettle-associated 'Ca. P. solani' genotypes are not commonly found
in BN-affected grapevine (Atanasova et al., 2015; Kosovac et al., 2016); hence, research on this
epidemiological pathway is neglected and available data are scarce. During more than a decade of
research on 'Ca. P. solani' epidemiology and H. obsoletus biology, ecology, and genetics in the
Balkans, we have frequently found the presence of "wrong" 'Ca. P. solani' genotypes in vector
specimens obtained from nettles. Here, we assess these findings and call into question the geographic
specificity of nettle-associated epidemiology in southeastern Europe.
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - Hyalesthes obsoletus and nettle-associated 'Ca. Phytoplasma solani' epidemiological cycle in Serbia and the Balkans: Is it closed and specific?
EP  - 21
SP  - 20
ER  - 

@conference{
author = "Jović, Jelena and Krstić, Oliver and Cvrković, Tatjana and Jakovljević, Miljana and Marinković, Slavica and Mitrović, Milana and Toševski, Ivo",
year = "2024",
abstract = "The planthopper Hyalesthes obsoletus Signoret, 1865 (Hemiptera: Cixiidae) is a major vector and
driver of 'Ca. Phytoplasma solani' epidemiology (Maixner, 1994; Jović & Toševski, 2023) and
associated diseases of cultivated plants, including the grapevine-Bois noir (BN) pathosystem. Because
of its strong association with its host plants as a subterranean nymph and short life span as an adult
(Cargnus et al., 2012), the epidemiological cycle is host plant-specific and is determined by the vector's
host range and the pathogen's reservoir range (Imo et al., 2013; Maixner et al., 2014). Although H.
obsoletus is commonly viewed as a polyphagous insect, there is plenty of proof of host plant adaptation
in its populations, which are referred to as host races, biotypes, or cryptic species (Imo et al., 2013;
Maixner et al., 2014; Kosovac et al., 2016; 2018; 2019). The first described and most prevalent
epidemiological cycles linked with distinct strains of 'Ca. P. solani' are driven by nettle and bindweed
as pathogen reservoir plants and H. obsoletus host plants (Langer & Maixner, 2004). The genetic
distinction between the two pathosystems was initially identified on the tuf gene and then verified by
secY, stamp, and vmp1 gene typing (Langer & Maixner, 2004; Johannesen et al., 2012; Aryan et al.,
2014). In Serbia and the Balkans, nettle-associated 'Ca. P. solani' genotypes are not commonly found
in BN-affected grapevine (Atanasova et al., 2015; Kosovac et al., 2016); hence, research on this
epidemiological pathway is neglected and available data are scarce. During more than a decade of
research on 'Ca. P. solani' epidemiology and H. obsoletus biology, ecology, and genetics in the
Balkans, we have frequently found the presence of "wrong" 'Ca. P. solani' genotypes in vector
specimens obtained from nettles. Here, we assess these findings and call into question the geographic
specificity of nettle-associated epidemiology in southeastern Europe.",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "Hyalesthes obsoletus and nettle-associated 'Ca. Phytoplasma solani' epidemiological cycle in Serbia and the Balkans: Is it closed and specific?",
pages = "21-20"
}

Jović, J., Krstić, O., Cvrković, T., Jakovljević, M., Marinković, S., Mitrović, M.,& Toševski, I.. (2024). Hyalesthes obsoletus and nettle-associated 'Ca. Phytoplasma solani' epidemiological cycle in Serbia and the Balkans: Is it closed and specific?. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 20-21.

Jović J, Krstić O, Cvrković T, Jakovljević M, Marinković S, Mitrović M, Toševski I. Hyalesthes obsoletus and nettle-associated 'Ca. Phytoplasma solani' epidemiological cycle in Serbia and the Balkans: Is it closed and specific?. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:20-21..

Jović, Jelena, Krstić, Oliver, Cvrković, Tatjana, Jakovljević, Miljana, Marinković, Slavica, Mitrović, Milana, Toševski, Ivo, "Hyalesthes obsoletus and nettle-associated 'Ca. Phytoplasma solani' epidemiological cycle in Serbia and the Balkans: Is it closed and specific?" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):20-21.

TY  - CONF
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Drčelić, Marina
AU  - Polak, Bruno
AU  - Lemaić, Zvonimira
AU  - Šeruga Musić, Martina
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1255
AB  - The occurrence of Flavescence dorée (FD) disease of grapevine, its associated phytoplasma, FDp (a
member of the 16SrV taxonomic group of elm yellows, subgroups V-C and -D), and the main insect
vector, the deltocephalinae leafhopper of North American origin, Scaphoideus titanus, was first
evidenced in vineyards in Croatia in 2009 (Šeruga Musić et al., 2011; Plavec et al., 2015).
Contemporary identification of FDp in the climbing shrub Clematis vitalba as one of the main natural
reservoir plants in North Italy and the Balkans was also confirmed for Croatia (Filippin et al., 2009).
Subsequent studies aimed at determining the genetic diversity and structure of FDp populations and
tracing transmission pathways in Croatia revealed the invasive tree Ailanthus altissima and the natural
riparian tree Alnus glutinosa as natural FDp reservoirs (Plavec et al., 2019). However, the roles and
epidemiological importance of each of the identified reservoir plants remained unclear due to the
limited number of elaborated samples. Regarding the natural insect vectors of FDp from reservoir
plants to grapevine and their association with natural reservoir plants on the wider territory of Croatia,
particularly beyond vineyards in their natural habitats, not much is known. To understand the
epidemiological importance of FDp natural plant hosts and insect vectors, we performed surveys
targeting clematis, alders, and other tentative natural woody hosts of FDp in vineyard surroundings
and natural habitats in wine-producing regions of Croatia.
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - Preliminary data on the natural reservoir plants and insect carriers of Flavescence dorée and related phytoplasmas in Croatia
EP  - 75
SP  - 74
ER  - 

@conference{
author = "Jović, Jelena and Krstić, Oliver and Cvrković, Tatjana and Drčelić, Marina and Polak, Bruno and Lemaić, Zvonimira and Šeruga Musić, Martina and Toševski, Ivo",
year = "2024",
abstract = "The occurrence of Flavescence dorée (FD) disease of grapevine, its associated phytoplasma, FDp (a
member of the 16SrV taxonomic group of elm yellows, subgroups V-C and -D), and the main insect
vector, the deltocephalinae leafhopper of North American origin, Scaphoideus titanus, was first
evidenced in vineyards in Croatia in 2009 (Šeruga Musić et al., 2011; Plavec et al., 2015).
Contemporary identification of FDp in the climbing shrub Clematis vitalba as one of the main natural
reservoir plants in North Italy and the Balkans was also confirmed for Croatia (Filippin et al., 2009).
Subsequent studies aimed at determining the genetic diversity and structure of FDp populations and
tracing transmission pathways in Croatia revealed the invasive tree Ailanthus altissima and the natural
riparian tree Alnus glutinosa as natural FDp reservoirs (Plavec et al., 2019). However, the roles and
epidemiological importance of each of the identified reservoir plants remained unclear due to the
limited number of elaborated samples. Regarding the natural insect vectors of FDp from reservoir
plants to grapevine and their association with natural reservoir plants on the wider territory of Croatia,
particularly beyond vineyards in their natural habitats, not much is known. To understand the
epidemiological importance of FDp natural plant hosts and insect vectors, we performed surveys
targeting clematis, alders, and other tentative natural woody hosts of FDp in vineyard surroundings
and natural habitats in wine-producing regions of Croatia.",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "Preliminary data on the natural reservoir plants and insect carriers of Flavescence dorée and related phytoplasmas in Croatia",
pages = "75-74"
}

Jović, J., Krstić, O., Cvrković, T., Drčelić, M., Polak, B., Lemaić, Z., Šeruga Musić, M.,& Toševski, I.. (2024). Preliminary data on the natural reservoir plants and insect carriers of Flavescence dorée and related phytoplasmas in Croatia. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 74-75.

Jović J, Krstić O, Cvrković T, Drčelić M, Polak B, Lemaić Z, Šeruga Musić M, Toševski I. Preliminary data on the natural reservoir plants and insect carriers of Flavescence dorée and related phytoplasmas in Croatia. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:74-75..

Jović, Jelena, Krstić, Oliver, Cvrković, Tatjana, Drčelić, Marina, Polak, Bruno, Lemaić, Zvonimira, Šeruga Musić, Martina, Toševski, Ivo, "Preliminary data on the natural reservoir plants and insect carriers of Flavescence dorée and related phytoplasmas in Croatia" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):74-75.

TY  - CONF
AU  - Jović, Jelena
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1254
AB  - The planthopper Reptalus artemisiae (Becker, 1865) (Hemiptera: Cixiidae) is an emerging vector of
'Ca. Phytoplasma solani', and a well studied species for nearly two decades for its tentative, and later
proven, vector role in phytoplasma transmission (Trivellone et al., 2005; Chuche et al., 2016).
However, until recently, there was a misidentification of this taxon, leading to the incorrect
nomenclature of Reptalus quinquecostatus (Dufour, 1833) (Webb et al., 2013), which is actually the
name of another cixiidae species, previously known as Reptalus melanochaetus (Fieber, 1872) and
now considered as its junior synonym. The case of misidentification was presented and documented
in detail by Webb and coauthors (2013), while the question of naming Reptalus quinquecostatus sensu
Fieber was resolved by Emeljanov (2020), who made the necessary nomenclatural changes. The
changes were made following the International Code of Zoological Nomenclature, Article 70.3. We
present here an overview of the (in)correct use of the taxon names for the two species in the last three
years since the changes were made and make a plea for using the correct taxon names.
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - A plea for using the correct taxon names of phytoplasma vectors: a case of Reptalus artemisiae, a vector of 'Candidatus Phytoplasma solani'
EP  - 45
SP  - 44
ER  - 

@conference{
author = "Jović, Jelena and Toševski, Ivo",
year = "2024",
abstract = "The planthopper Reptalus artemisiae (Becker, 1865) (Hemiptera: Cixiidae) is an emerging vector of
'Ca. Phytoplasma solani', and a well studied species for nearly two decades for its tentative, and later
proven, vector role in phytoplasma transmission (Trivellone et al., 2005; Chuche et al., 2016).
However, until recently, there was a misidentification of this taxon, leading to the incorrect
nomenclature of Reptalus quinquecostatus (Dufour, 1833) (Webb et al., 2013), which is actually the
name of another cixiidae species, previously known as Reptalus melanochaetus (Fieber, 1872) and
now considered as its junior synonym. The case of misidentification was presented and documented
in detail by Webb and coauthors (2013), while the question of naming Reptalus quinquecostatus sensu
Fieber was resolved by Emeljanov (2020), who made the necessary nomenclatural changes. The
changes were made following the International Code of Zoological Nomenclature, Article 70.3. We
present here an overview of the (in)correct use of the taxon names for the two species in the last three
years since the changes were made and make a plea for using the correct taxon names.",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "A plea for using the correct taxon names of phytoplasma vectors: a case of Reptalus artemisiae, a vector of 'Candidatus Phytoplasma solani'",
pages = "45-44"
}

Jović, J.,& Toševski, I.. (2024). A plea for using the correct taxon names of phytoplasma vectors: a case of Reptalus artemisiae, a vector of 'Candidatus Phytoplasma solani'. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 44-45.

Jović J, Toševski I. A plea for using the correct taxon names of phytoplasma vectors: a case of Reptalus artemisiae, a vector of 'Candidatus Phytoplasma solani'. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:44-45..

Jović, Jelena, Toševski, Ivo, "A plea for using the correct taxon names of phytoplasma vectors: a case of Reptalus artemisiae, a vector of 'Candidatus Phytoplasma solani'" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):44-45.

TY  - CONF
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Toševski, Ivo
AU  - Drčelić, Marina
AU  - Šeruga Musić, Martina
AU  - Jović, Jelena
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1253
AB  - Bois noir (BN) disease of grapevine ('Ca. Phytoplasma solani', CaPsol,16S rRNA XII-A) was first
molecularly confirmed to affect vineyards in Croatia more than two decades ago (Škorić et al., 1998).
Presently, BN is widespread, occurring in most of the wine regions of the country (Plavec et al., 2018).
The diversity of CaPsol genotypes in Croatian vineyards indicates complex epidemiology, which was
recently confirmed in Istria, the westernmost grape-growing county of Croatia in the northeastern
Adriatic Sea (Delač et al., 2023). Euscelis incisus (Kirschbaum, 1858) is a nitrophilous, polyphagous
leafhopper, widely distributed throughout the Western Palaearctic region, inhabiting diverse habitats
(Nickel, 2003). Due to its wide distribution and ability to inhabit a variety of ecologically distinct
environments, this leafhopper can encounter diverse phytoplasmas that invade a wide range of plant
species. It has been established as a vector of the 16Sr I-B/C, III-B, and XII-A phytoplasma subgroups
and a carrier of the I-F/R, II-E, IX-C/E, and XI-G subgroups (Jakovljević et al., 2020). Its congener,
E. lineolatus (Brullé, 1832) was found to harbor I-B/C and XII-A subgroups, while its ability to act as
a phytoplasma vector has not been confirmed so far (Landi et al., 2013). The aim of this study was to
investigate the epidemiological and vector roles of E. incisus and co-occurring E. lineolatus in
transmitting CaPsol in BN-affected vineyards of Istria.
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - Euscelis incisus in Bois noir-affected vineyards of Istria (Croatia): vector competence and syntopic occurrence with E. lineolatus
EP  - 41
SP  - 40
ER  - 

@conference{
author = "Jakovljević, Miljana and Cvrković, Tatjana and Toševski, Ivo and Drčelić, Marina and Šeruga Musić, Martina and Jović, Jelena",
year = "2024",
abstract = "Bois noir (BN) disease of grapevine ('Ca. Phytoplasma solani', CaPsol,16S rRNA XII-A) was first
molecularly confirmed to affect vineyards in Croatia more than two decades ago (Škorić et al., 1998).
Presently, BN is widespread, occurring in most of the wine regions of the country (Plavec et al., 2018).
The diversity of CaPsol genotypes in Croatian vineyards indicates complex epidemiology, which was
recently confirmed in Istria, the westernmost grape-growing county of Croatia in the northeastern
Adriatic Sea (Delač et al., 2023). Euscelis incisus (Kirschbaum, 1858) is a nitrophilous, polyphagous
leafhopper, widely distributed throughout the Western Palaearctic region, inhabiting diverse habitats
(Nickel, 2003). Due to its wide distribution and ability to inhabit a variety of ecologically distinct
environments, this leafhopper can encounter diverse phytoplasmas that invade a wide range of plant
species. It has been established as a vector of the 16Sr I-B/C, III-B, and XII-A phytoplasma subgroups
and a carrier of the I-F/R, II-E, IX-C/E, and XI-G subgroups (Jakovljević et al., 2020). Its congener,
E. lineolatus (Brullé, 1832) was found to harbor I-B/C and XII-A subgroups, while its ability to act as
a phytoplasma vector has not been confirmed so far (Landi et al., 2013). The aim of this study was to
investigate the epidemiological and vector roles of E. incisus and co-occurring E. lineolatus in
transmitting CaPsol in BN-affected vineyards of Istria.",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "Euscelis incisus in Bois noir-affected vineyards of Istria (Croatia): vector competence and syntopic occurrence with E. lineolatus",
pages = "41-40"
}

Jakovljević, M., Cvrković, T., Toševski, I., Drčelić, M., Šeruga Musić, M.,& Jović, J.. (2024). Euscelis incisus in Bois noir-affected vineyards of Istria (Croatia): vector competence and syntopic occurrence with E. lineolatus. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 40-41.

Jakovljević M, Cvrković T, Toševski I, Drčelić M, Šeruga Musić M, Jović J. Euscelis incisus in Bois noir-affected vineyards of Istria (Croatia): vector competence and syntopic occurrence with E. lineolatus. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:40-41..

Jakovljević, Miljana, Cvrković, Tatjana, Toševski, Ivo, Drčelić, Marina, Šeruga Musić, Martina, Jović, Jelena, "Euscelis incisus in Bois noir-affected vineyards of Istria (Croatia): vector competence and syntopic occurrence with E. lineolatus" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):40-41.

TY  - CONF
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1252
AB  - Flavescence dorée (FD) is a severe grapevine disease caused by FD phytoplasmas (FDp), impacting
major viticultural regions in Europe. The epidemic spread of FD within vineyards is facilitated by the
Nearctic leafhopper Scaphoideus titanus, completing its life cycle on Vitis sp. plants in Europe
(Chuche & Thiéry, 2014). Through genotyping of the informative house-keeping gene map, FDp has
been categorized into three genetic clusters, each associated with specific vector and reservoir plant
epidemiology or geographic distribution (Arnaud et al., 2007; Malembic-Maher et al., 2020).
Genotypes of the Map-FD2 cluster are most abundant in vineyards in France, Switzerland, Italy,
Croatia, and Slovenia, while FD3 prevails in the Balkans (reviewed in Krstić et al., 2022). Epidemic
outbreaks of FD in Serbian vineyards began in the early 2000s, and despite extensive preventive and
curative measures, today FD is affecting vineyard areas in all administrative districts in Serbia(Krstić
et al., 2022).Until recently, the M51 genotype belonging to the Map-FD3 cluster, which is common in
all districts and wine-growing regions, was the only genotype associated with the FDp outbreaks in
Serbian vineyards.Subsequent surveys in the late 2010s on the occurrence and diversityof FDp in
Serbian vineyards revealed for the first timetheoccurrence of other genotypes, including members of
the Map-FD2 cluster: M89, M148, and M155, indicating that FDp in Serbian vineyards is not
monotypic and has a complex epidemiology(Krstić et al., 2022). This discovery prompted a more indepth
investigation into the specific ecological factors contributing to disease outbreaks to assess the
significance of these new findings. Initial results from this research are presented herein.
PB  - UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE
C3  - Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
T1  - New insights on Flavescence dorée epidemiology in Serbia
EP  - 77
SP  - 76
ER  - 

@conference{
author = "Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Jakovljević, Miljana and Marinković, Slavica and Mitrović, Milana and Toševski, Ivo",
year = "2024",
abstract = "Flavescence dorée (FD) is a severe grapevine disease caused by FD phytoplasmas (FDp), impacting
major viticultural regions in Europe. The epidemic spread of FD within vineyards is facilitated by the
Nearctic leafhopper Scaphoideus titanus, completing its life cycle on Vitis sp. plants in Europe
(Chuche & Thiéry, 2014). Through genotyping of the informative house-keeping gene map, FDp has
been categorized into three genetic clusters, each associated with specific vector and reservoir plant
epidemiology or geographic distribution (Arnaud et al., 2007; Malembic-Maher et al., 2020).
Genotypes of the Map-FD2 cluster are most abundant in vineyards in France, Switzerland, Italy,
Croatia, and Slovenia, while FD3 prevails in the Balkans (reviewed in Krstić et al., 2022). Epidemic
outbreaks of FD in Serbian vineyards began in the early 2000s, and despite extensive preventive and
curative measures, today FD is affecting vineyard areas in all administrative districts in Serbia(Krstić
et al., 2022).Until recently, the M51 genotype belonging to the Map-FD3 cluster, which is common in
all districts and wine-growing regions, was the only genotype associated with the FDp outbreaks in
Serbian vineyards.Subsequent surveys in the late 2010s on the occurrence and diversityof FDp in
Serbian vineyards revealed for the first timetheoccurrence of other genotypes, including members of
the Map-FD2 cluster: M89, M148, and M155, indicating that FDp in Serbian vineyards is not
monotypic and has a complex epidemiology(Krstić et al., 2022). This discovery prompted a more indepth
investigation into the specific ecological factors contributing to disease outbreaks to assess the
significance of these new findings. Initial results from this research are presented herein.",
publisher = "UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE",
journal = "Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024",
title = "New insights on Flavescence dorée epidemiology in Serbia",
pages = "77-76"
}

Cvrković, T., Jovic, J., Krstić, O., Jakovljević, M., Marinković, S., Mitrović, M.,& Toševski, I.. (2024). New insights on Flavescence dorée epidemiology in Serbia. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024
UMR-1332 Fruit Biology and Pathology, University of Bordeaux and INRAE., 76-77.

Cvrković T, Jovic J, Krstić O, Jakovljević M, Marinković S, Mitrović M, Toševski I. New insights on Flavescence dorée epidemiology in Serbia. in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024. 2024;:76-77..

Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Jakovljević, Miljana, Marinković, Slavica, Mitrović, Milana, Toševski, Ivo, "New insights on Flavescence dorée epidemiology in Serbia" in Proceedings of the 6th European Bois Noir workshop and 1st International Pro-AECOGY conference 14-16 may, 2024 (2024):76-77.

TY  - JOUR
AU  - Gosik, Rafal
AU  - Caldara, Roberto
AU  - Toševski, Ivo
AU  - Skuhrovec, Jiří
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1227
AB  - The mature larvae of the following fourteen Rhinusa species are described and illustrated:
Rhinusa antirrhini (Paykull, 1800), R. asellus (Gravenhorst, 1807), R. collina (Gyllenhal,
1813), R. eversmanni (Rosenschoeld, 1838), R. florum (Rubsaamen, 1895), R. herbarum
(H. Brisout de Barneville, 1862), R. incana (Kirsch, 1881), R. linariae (Panzer, 1796),
R. melas (Boheman, 1838), R. neta (Germar, 1821), R. pilosa (Gyllenhal, 1838), R. rara
Toševski & Caldara, 2015, R. tetra (Fabricius, 1792), and R. vestita (Germar, 1821). The
pupae of thirteen of them (except R. incana) were also described. The comparison of
larval morphological characters and plant preferences provides evidence supporting
the existence of different species groups previously established according to a phylogenetic
analysis based on adult morphological characters. The following diagnostic
attributes distinguishing the genus Rhinusa are highlighted. For the larvae: (1) pronotal
shield indistinct; (2) thoracic prodorsal fold small or even vestigial; (3) abdominal postdorsal
folds (especially of segments III–VII) high or even in the form of conical protuberances;
(4) cuticle of abdominal segments densely covered with asperities; (5) cuticle
without dark spots or dark pigmentation; (6) head suboval, rarely round; (7) labrum usually
with 2 als; (8) des1 short or absent, rarely elongated; and (9) fs1-3 usually absent or
minute. For the pupae: (1) body stout; (2) head protuberances always present; (3) pronotal
protuberances (if present), separated at bases of the pronotum, always wider than
higher; (4) abdominal protuberance usually present, wide or round; (5) femora usually
with a single fes; and (6) urogomphi short or vestigial. Keys to the larvae and pupae
described here are provided. All the characters used for identification are illustrated by
photographs or drawings. Biological and distribution data, including new information,
are provided for all the species studied.
PB  - Pensoft Publishers
T2  - ZooKeys
T1  - Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels
EP  - 94
SP  - 1
VL  - 1195
DO  - 10.3897/zookeys.1195.112328
ER  - 

@article{
author = "Gosik, Rafal and Caldara, Roberto and Toševski, Ivo and Skuhrovec, Jiří",
year = "2024",
abstract = "The mature larvae of the following fourteen Rhinusa species are described and illustrated:
Rhinusa antirrhini (Paykull, 1800), R. asellus (Gravenhorst, 1807), R. collina (Gyllenhal,
1813), R. eversmanni (Rosenschoeld, 1838), R. florum (Rubsaamen, 1895), R. herbarum
(H. Brisout de Barneville, 1862), R. incana (Kirsch, 1881), R. linariae (Panzer, 1796),
R. melas (Boheman, 1838), R. neta (Germar, 1821), R. pilosa (Gyllenhal, 1838), R. rara
Toševski & Caldara, 2015, R. tetra (Fabricius, 1792), and R. vestita (Germar, 1821). The
pupae of thirteen of them (except R. incana) were also described. The comparison of
larval morphological characters and plant preferences provides evidence supporting
the existence of different species groups previously established according to a phylogenetic
analysis based on adult morphological characters. The following diagnostic
attributes distinguishing the genus Rhinusa are highlighted. For the larvae: (1) pronotal
shield indistinct; (2) thoracic prodorsal fold small or even vestigial; (3) abdominal postdorsal
folds (especially of segments III–VII) high or even in the form of conical protuberances;
(4) cuticle of abdominal segments densely covered with asperities; (5) cuticle
without dark spots or dark pigmentation; (6) head suboval, rarely round; (7) labrum usually
with 2 als; (8) des1 short or absent, rarely elongated; and (9) fs1-3 usually absent or
minute. For the pupae: (1) body stout; (2) head protuberances always present; (3) pronotal
protuberances (if present), separated at bases of the pronotum, always wider than
higher; (4) abdominal protuberance usually present, wide or round; (5) femora usually
with a single fes; and (6) urogomphi short or vestigial. Keys to the larvae and pupae
described here are provided. All the characters used for identification are illustrated by
photographs or drawings. Biological and distribution data, including new information,
are provided for all the species studied.",
publisher = "Pensoft Publishers",
journal = "ZooKeys",
title = "Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels",
pages = "94-1",
volume = "1195",
doi = "10.3897/zookeys.1195.112328"
}

Gosik, R., Caldara, R., Toševski, I.,& Skuhrovec, J.. (2024). Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels. in ZooKeys
Pensoft Publishers., 1195, 1-94.
https://doi.org/10.3897/zookeys.1195.112328

Gosik R, Caldara R, Toševski I, Skuhrovec J. Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels. in ZooKeys. 2024;1195:1-94.
doi:10.3897/zookeys.1195.112328 .

Gosik, Rafal, Caldara, Roberto, Toševski, Ivo, Skuhrovec, Jiří, "Description of immature stages of Rhinusa species (Coleoptera, Curculionidae, Mecinini) with a focus on diagnostic morphological characters at the species and genus levels" in ZooKeys, 1195 (2024):1-94,
https://doi.org/10.3897/zookeys.1195.112328 . .

TY  - CONF
AU  - Jović, Jelena
AU  - Cvrković, Tatjana
AU  - Krstić, Oliver
AU  - Mitrović, Milana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Toševski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1215
AB  - “Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.
C3  - European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy
T1  - Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia
EP  - 54
SP  - 53
ER  - 

@conference{
author = "Jović, Jelena and Cvrković, Tatjana and Krstić, Oliver and Mitrović, Milana and Jakovljević, Miljana and Marinković, Slavica and Toševski, Ivo",
year = "2024",
abstract = "“Flavescence dorée” (FD) outbreaks in Serbian vineyards first emerged in the
early 2000s and were directly correlated with dense populations of the North
American insect vector Scaphoideus titanus. The FD phytoplasma associated
with the disease was first treated as alien and introduced, like it happens for
its insect vector. However, the genetic peculiarities of FD phytoplasma strains
from Serbia, as well as their occurrence in native alder trees in riparian areas
and clematis plants in vineyard surroundings and in natural habitats, proved
otherwise. Molecular data on the ecological properties of epidemiological
cycles driving the epidemic outbreaks of FD in Serbia today indicated the
importance of alders as reservoir plants for the epidemics (Krstić et al., 2022).
Riparian habitats hosting alder trees, alongside wild Vitis, Salix sp., clematis,
tree of heaven, and other FD phytoplasmas reservoir plants, were screened for
native leafhopper vectors as well as for Orientus ishidae, a newly established
vector of Asian origin in Serbia (Cvrković et al., 2021). Leafhopper Allygus
modestus and A. mixtus were commonly found in association with alders
in many sites, with the former being the dominant species. An established
population of O. ishidae feeding on alders was found in a single location of
riparian habitat in eastern Serbia. Among the analyzed leafhopper specimens,
using epidemiologically informative map gene typing, nearly all A. modestus
individuals were found carrying FD or -related phytoplasmas, while nearly
50% of O. ishidae were found infected at the same location. However,
A. modestus specimens had a lower phytoplasma load (Cq > 25) than O. ishidae
(Cq < 20 in 50% of infected individuals), even though they were collected in
the same period of the year. When comparing the map genotypes carried
by each of the putative vector species, A. modestus most often carried AldY genotypes, while O. ishidae was dominantly found harboring FD phytoplasma
genotypes of both Map-FD1 and FD2 clusters. The experimental verification
of the transmission capability and role in FD phytoplasma epidemiology for
each of the leafhopper species is under study.",
journal = "European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy",
title = "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia",
pages = "54-53"
}

Jović, J., Cvrković, T., Krstić, O., Mitrović, M., Jakovljević, M., Marinković, S.,& Toševski, I.. (2024). Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy, 53-54.

Jović J, Cvrković T, Krstić O, Mitrović M, Jakovljević M, Marinković S, Toševski I. Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy. 2024;:53-54..

Jović, Jelena, Cvrković, Tatjana, Krstić, Oliver, Mitrović, Milana, Jakovljević, Miljana, Marinković, Slavica, Toševski, Ivo, "Insect vectors of "flavescence dorée" and related phytoplasmas in natural areas of riparian habitats in Serbia" in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy (2024):53-54.

TY  - CONF
AU  - Cvrković, Tatjana
AU  - Jović, Jelena
AU  - Jakovljević, Miljana
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1214
AB  - “Flavescence dorée” (FD) is a European quarantine disease affecting grapevines,
associated with the “flavescence dorée” phytoplasma and transmitted by
the North American leafhopper, Scaphoideus titanus. This pathogen has a
significant impact on the major viticultural areas across Europe, as well as all
wine-producing regions in Serbia. The occurrence of S. titanus was initially
recorded in Serbian vineyards in 2002 (Magud and Toševski, 2004). However,
the high population density observed at that time suggests that the vector’s
presence likely dates back to the 1990s (Ivo Toševski, unpublished data).
Despite the extensive measures to contain the pathogen, the encompassing
disease and vector surveillance, the mandatory insecticidal treatments against
S. titanus, and the utilization of certified phytoplasma-free planting material,
the disease continues to spread. Presently, FD affects vineyards in all districts of
Serbia (Krstić et al., 2022). A recent two-year survey (2022-2023) on S. titanus
distribution in both wild and cultivated areas within vineyard ecosystems
revealed widespread prevalence of the vector across all grapevine-growing
regions in Serbia. High population densities were observed in districts where
Map-FD3 genotypes are common. These include grape-producing regions all
over Serbia where the autochthonous M51 genotype is dominant, hosted by
grapevine, along with reservoir plants Clematis vitalba and Ailanthus altissima.
Additionally, substantial S. titanus populations were registered in northern
Serbia, where multiple outbreaks of the M12 genotype occurred, and central
Serbia, where five new genotypes (M150-M154) were detected in grapevines,
indicating a high endemic potential for new outbreaks in these regions. Finally,
significant populations of the insect vector were discovered in riparian habitats
near rivers in Podunavlje and Mačva, where two “flavescence dorée” endemic genotypes of the Map-FD2 (M89 and M148) were found in grapevines (Krstić
et al., 2022). The current phytosanitary situation in Serbian vineyards provides
evidence that, despite measures implemented over the past two decades, there
has been no substantial reduction in the population density and spread of S.
titanus. Due to the high complexity of the “flavescence dorée” epidemiological
cycle caused by natural reservoir plants and native or introduced insect vectors,
linking natural habitats with adjacent vineyard ecosystems, managing ongoing
epidemics and preventing new outbreaks in the Balkans remains a challenge.
C3  - European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy
T1  - Status of Scaphoideus titanus in Serbia vineyards two decades later
EP  - 42
SP  - 41
ER  - 

@conference{
author = "Cvrković, Tatjana and Jović, Jelena and Jakovljević, Miljana and Krstić, Oliver and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo",
year = "2024",
abstract = "“Flavescence dorée” (FD) is a European quarantine disease affecting grapevines,
associated with the “flavescence dorée” phytoplasma and transmitted by
the North American leafhopper, Scaphoideus titanus. This pathogen has a
significant impact on the major viticultural areas across Europe, as well as all
wine-producing regions in Serbia. The occurrence of S. titanus was initially
recorded in Serbian vineyards in 2002 (Magud and Toševski, 2004). However,
the high population density observed at that time suggests that the vector’s
presence likely dates back to the 1990s (Ivo Toševski, unpublished data).
Despite the extensive measures to contain the pathogen, the encompassing
disease and vector surveillance, the mandatory insecticidal treatments against
S. titanus, and the utilization of certified phytoplasma-free planting material,
the disease continues to spread. Presently, FD affects vineyards in all districts of
Serbia (Krstić et al., 2022). A recent two-year survey (2022-2023) on S. titanus
distribution in both wild and cultivated areas within vineyard ecosystems
revealed widespread prevalence of the vector across all grapevine-growing
regions in Serbia. High population densities were observed in districts where
Map-FD3 genotypes are common. These include grape-producing regions all
over Serbia where the autochthonous M51 genotype is dominant, hosted by
grapevine, along with reservoir plants Clematis vitalba and Ailanthus altissima.
Additionally, substantial S. titanus populations were registered in northern
Serbia, where multiple outbreaks of the M12 genotype occurred, and central
Serbia, where five new genotypes (M150-M154) were detected in grapevines,
indicating a high endemic potential for new outbreaks in these regions. Finally,
significant populations of the insect vector were discovered in riparian habitats
near rivers in Podunavlje and Mačva, where two “flavescence dorée” endemic genotypes of the Map-FD2 (M89 and M148) were found in grapevines (Krstić
et al., 2022). The current phytosanitary situation in Serbian vineyards provides
evidence that, despite measures implemented over the past two decades, there
has been no substantial reduction in the population density and spread of S.
titanus. Due to the high complexity of the “flavescence dorée” epidemiological
cycle caused by natural reservoir plants and native or introduced insect vectors,
linking natural habitats with adjacent vineyard ecosystems, managing ongoing
epidemics and preventing new outbreaks in the Balkans remains a challenge.",
journal = "European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy",
title = "Status of Scaphoideus titanus in Serbia vineyards two decades later",
pages = "42-41"
}

Cvrković, T., Jović, J., Jakovljević, M., Krstić, O., Marinković, S., Mitrović, M.,& Tosevski, I.. (2024). Status of Scaphoideus titanus in Serbia vineyards two decades later. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy, 41-42.

Cvrković T, Jović J, Jakovljević M, Krstić O, Marinković S, Mitrović M, Tosevski I. Status of Scaphoideus titanus in Serbia vineyards two decades later. in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy. 2024;:41-42..

Cvrković, Tatjana, Jović, Jelena, Jakovljević, Miljana, Krstić, Oliver, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, "Status of Scaphoideus titanus in Serbia vineyards two decades later" in European workshop on "Flavescence dorée", Recent acquisitions and management strategies, 25-26 January 2024, Verona, Italy (2024):41-42.

TY  - CONF
AU  - Delač, Magdalena
AU  - Polak, Bruno
AU  - Drčelić, Marina
AU  - Jović, Jelena
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Šeruga Musić, Martina
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1216
AB  - Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.
C3  - Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023
T1  - Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich
EP  - 185
SP  - 184
ER  - 

@conference{
author = "Delač, Magdalena and Polak, Bruno and Drčelić, Marina and Jović, Jelena and Krstić, Oliver and Toševski, Ivo and Cvrković, Tatjana and Jakovljević, Miljana and Šeruga Musić, Martina",
year = "2023",
abstract = "Grapevine Yellows (GY) are economically important diseases associated to phytoplasmas (genus ‘Candidatus
Phytoplasma’), uncultivable bacteria from the class Mollicutes. In Euro-mediterranean region, GY are mainly
attributed to phytoplasmas belonging to ribosomal 16SrXII-A subgroup ('Ca. P. solani'; Bois Noir phytoplasma;
BNp) and 16SrV group (flavescence dorée phytoplasma; FDp), with the latter one being a quarantine pathogen
(Plavec et al. 2015, Quaglino et al. 2013). Although symptomatology can be similar, epidemiology of FDp and
BNp is very different as they are transmitted by different insect vectors and could have different alternative
hosts. In Croatia, GY diseases have been continuously monitored since 1997 with FDp appearing only in 2009
(Šeruga Musić et al. 2011). While BNp is more diverse and widespread, FDp belonging to 3 map phylogenetic
clusters are differently distributed throughout regions. Istria is heavily affected with FDp since 2014 with only
one genotype (mapFD2/M54) being attributed to great losses (Plavec et al. 2019). On the contrary, information
on BNp diversity is scarce with only couple of genotypes detected so far (Plavec et al, manuscript in
preparation). Therefore, the aim of this case study was to assess the diversity of BNp genotypes in a small
selected area of Istria, in order to draw attention to the presence and importance of BNp as GY agent in this
important Croatian viticultural region.",
journal = "Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023",
title = "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich",
pages = "185-184"
}

Delač, M., Polak, B., Drčelić, M., Jović, J., Krstić, O., Toševski, I., Cvrković, T., Jakovljević, M.,& Šeruga Musić, M.. (2023). Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023, 184-185.

Delač M, Polak B, Drčelić M, Jović J, Krstić O, Toševski I, Cvrković T, Jakovljević M, Šeruga Musić M. Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich. in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023. 2023;:184-185..

Delač, Magdalena, Polak, Bruno, Drčelić, Marina, Jović, Jelena, Krstić, Oliver, Toševski, Ivo, Cvrković, Tatjana, Jakovljević, Miljana, Šeruga Musić, Martina, "Hidden genetic diversity of ‘Candidatus Phytoplasma solani’ strains in Istrian vineyards: how small can be rich" in Proceedings of the 20th Congress of the International Council for the Study of Virus and Virus-like diseases of the Grapevine (ICVG), Thessaloniki, Greece 25-29 September, 2023 (2023):184-185.

TY  - JOUR
AU  - McClay, Alec
AU  - Bourchier, Robert
AU  - Landry, Jean-François
AU  - Morris, Catherine
AU  - Tosevski, Ivo
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1153
AB  - The European root-boring moth, Chamaesphecia empiformis (Lepidoptera: Sesiidae), was released in Ontario, Canada, in 1971 and 1989 as a biological control agent for the perennial invasive weed cypress spurge, Euphorbia cyparissias Linnaeus (Euphorbiaceae). Until recently, the moth was believed to have failed to establish. Beginning in 2015, images posted on citizen science platforms, including BugGuide, iNaturalist, and Facebook groups, indicated that C. empiformis was in fact established. We have confirmed its presence in eastern Ontario by morphological and molecular identification of field-collected adults and eggs. This may be the longest-known lapse between the release of a weed biological control agent and confirmation of its establishment. Citizen science may provide valuable records documenting the establishment and dispersal of biological control agents.
PB  - Entomological Society of Canada
T2  - The Canadian Entomologist
T1  - Citizen science reveals the establishment of Chamaesphecia empiformis (Esper) (Lepidoptera: Sesiidae), a long-lost biological control agent for Euphorbia cyparissias (Euphorbiaceae), in Ontario, Canada
VL  - 155
DO  - 10.4039/tce.2023.17
ER  - 

@article{
author = "McClay, Alec and Bourchier, Robert and Landry, Jean-François and Morris, Catherine and Tosevski, Ivo",
year = "2023",
abstract = "The European root-boring moth, Chamaesphecia empiformis (Lepidoptera: Sesiidae), was released in Ontario, Canada, in 1971 and 1989 as a biological control agent for the perennial invasive weed cypress spurge, Euphorbia cyparissias Linnaeus (Euphorbiaceae). Until recently, the moth was believed to have failed to establish. Beginning in 2015, images posted on citizen science platforms, including BugGuide, iNaturalist, and Facebook groups, indicated that C. empiformis was in fact established. We have confirmed its presence in eastern Ontario by morphological and molecular identification of field-collected adults and eggs. This may be the longest-known lapse between the release of a weed biological control agent and confirmation of its establishment. Citizen science may provide valuable records documenting the establishment and dispersal of biological control agents.",
publisher = "Entomological Society of Canada",
journal = "The Canadian Entomologist",
title = "Citizen science reveals the establishment of Chamaesphecia empiformis (Esper) (Lepidoptera: Sesiidae), a long-lost biological control agent for Euphorbia cyparissias (Euphorbiaceae), in Ontario, Canada",
volume = "155",
doi = "10.4039/tce.2023.17"
}

McClay, A., Bourchier, R., Landry, J., Morris, C.,& Tosevski, I.. (2023). Citizen science reveals the establishment of Chamaesphecia empiformis (Esper) (Lepidoptera: Sesiidae), a long-lost biological control agent for Euphorbia cyparissias (Euphorbiaceae), in Ontario, Canada. in The Canadian Entomologist
Entomological Society of Canada., 155.
https://doi.org/10.4039/tce.2023.17

McClay A, Bourchier R, Landry J, Morris C, Tosevski I. Citizen science reveals the establishment of Chamaesphecia empiformis (Esper) (Lepidoptera: Sesiidae), a long-lost biological control agent for Euphorbia cyparissias (Euphorbiaceae), in Ontario, Canada. in The Canadian Entomologist. 2023;155.
doi:10.4039/tce.2023.17 .

McClay, Alec, Bourchier, Robert, Landry, Jean-François, Morris, Catherine, Tosevski, Ivo, "Citizen science reveals the establishment of Chamaesphecia empiformis (Esper) (Lepidoptera: Sesiidae), a long-lost biological control agent for Euphorbia cyparissias (Euphorbiaceae), in Ontario, Canada" in The Canadian Entomologist, 155 (2023),
https://doi.org/10.4039/tce.2023.17 . .

Marić, S., Jovic, J., Sušnik-Bajec, S., Tosevski, I.,& Stanković, D.. (2023). Molekularna sistematika - odabrane oblasti. in Univerzitet u Beogradu, Biološki fakultet
Beograd : Univerzitet, Biološki fakultet..

Marić S, Jovic J, Sušnik-Bajec S, Tosevski I, Stanković D. Molekularna sistematika - odabrane oblasti. in Univerzitet u Beogradu, Biološki fakultet. 2023;..

Marić, Saša, Jovic, Jelena, Sušnik-Bajec, Simona, Tosevski, Ivo, Stanković, David, "Molekularna sistematika - odabrane oblasti" in Univerzitet u Beogradu, Biološki fakultet (2023).

TY  - JOUR
AU  - Gosik, Rafał
AU  - Sprick, Peter
AU  - Wrzesien, Malgorzata
AU  - Dzyr, Agata
AU  - Krstić, Oliver
AU  - Tosevski, Ivo
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/711
AB  - The mature larva and pupa of Otiorhynchus smreczynskii are described and illustrated with complete chaetotaxy for the first time. Five larval instars and the factors for larval growth are determined, and the larval development of this species is comprehensively described. In order to confirm species affiliation, selected larvae were subjected to genetic analysis (mtCOI). Host plants and unique feeding signs of some Entiminae species are presented, and all available data on development are documented and interpreted. Additionally, the morphometry of 78 specimens (48 of O. smreczynskii and 30 of O. rotundus) was examined in order to verify the usefulness of morphological features in distinguishing both species. The female genitalia of both species are illustrated, described and compared with each other for the first time. Finally, the updated distribution of O. smreczynskii is given, and a possible origin of O. smreczynskii and O. rotundus is proposed.
PB  - MDPI Basel
T2  - INSECTS
T1  - Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution
IS  - 4
VL  - 14
DO  - 10.3390/insects14040360
ER  - 

@article{
author = "Gosik, Rafał and Sprick, Peter and Wrzesien, Malgorzata and Dzyr, Agata and Krstić, Oliver and Tosevski, Ivo",
year = "2023",
abstract = "The mature larva and pupa of Otiorhynchus smreczynskii are described and illustrated with complete chaetotaxy for the first time. Five larval instars and the factors for larval growth are determined, and the larval development of this species is comprehensively described. In order to confirm species affiliation, selected larvae were subjected to genetic analysis (mtCOI). Host plants and unique feeding signs of some Entiminae species are presented, and all available data on development are documented and interpreted. Additionally, the morphometry of 78 specimens (48 of O. smreczynskii and 30 of O. rotundus) was examined in order to verify the usefulness of morphological features in distinguishing both species. The female genitalia of both species are illustrated, described and compared with each other for the first time. Finally, the updated distribution of O. smreczynskii is given, and a possible origin of O. smreczynskii and O. rotundus is proposed.",
publisher = "MDPI Basel",
journal = "INSECTS",
title = "Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution",
number = "4",
volume = "14",
doi = "10.3390/insects14040360"
}

Gosik, R., Sprick, P., Wrzesien, M., Dzyr, A., Krstić, O.,& Tosevski, I.. (2023). Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution. in INSECTS
MDPI Basel., 14(4).
https://doi.org/10.3390/insects14040360

Gosik R, Sprick P, Wrzesien M, Dzyr A, Krstić O, Tosevski I. Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution. in INSECTS. 2023;14(4).
doi:10.3390/insects14040360 .

Gosik, Rafał, Sprick, Peter, Wrzesien, Malgorzata, Dzyr, Agata, Krstić, Oliver, Tosevski, Ivo, "Developmental Biology and Identification of a Garden Pest, Otiorhynchus (Podoropelmus) smreczynskii Cmoluch, 1968 (Coleoptera, Curculionidae, Entiminae), with Comments on Its Origin and Distribution" in INSECTS, 14, no. 4 (2023),
https://doi.org/10.3390/insects14040360 . .

TY  - JOUR
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/710
AB  - Given the ecological and epidemiological specialization of Hyalesthes obsoletus, the principle vector of ‘Candidatus Phytoplasma solani’, the primary objective of this study was to develop molecular tools for discriminating three host plant associations of the vector populations: (i) Convolvulus arvensis–Urtica dioica (Ca–Ud), (ii) Vitex agnus-castus (Vac), and (iii) Crepis foetida (Cf). The genetic diversity of the nearly full-length mitochondrial COI gene (1467 bp) was analyzed and compared among previously reported and newly collected individuals of the three host plant associations on a wide geographic range. Multiplex PCR was designed and evaluated for discriminating H. obsoletus host plant associations based on the size of amplified fragments: 1084 bp for the Cf association, 645 bp for the Ca–Ud association, and 355 bp for the Vac association. Examples of the epidemiological value of combining data on the genetic characteristics of the vector and the pathogen are provided. The method is intended to facilitate an accurate identification of the vector’s phylogenetic lineage, natural host plant preference, and epidemiological transmission routes of ‘Ca. P. solani’. When applied to H. obsoletus specimens collected from cultivated plants within an agroecosystem and combined with ‘Ca. P. solani’ genotyping, the method should provide valuable information on disease epidemiology, source(s) of emergence, and transmission routes.
PB  - MDPI, Basel
T2  - AGRONOMY-BASEL
T1  - Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of 'Ca. Phytoplasma solani' Epidemiology
IS  - 3
VL  - 13
DO  - 10.3390/agronomy13030831
ER  - 

@article{
author = "Jovic, Jelena and Tosevski, Ivo",
year = "2023",
abstract = "Given the ecological and epidemiological specialization of Hyalesthes obsoletus, the principle vector of ‘Candidatus Phytoplasma solani’, the primary objective of this study was to develop molecular tools for discriminating three host plant associations of the vector populations: (i) Convolvulus arvensis–Urtica dioica (Ca–Ud), (ii) Vitex agnus-castus (Vac), and (iii) Crepis foetida (Cf). The genetic diversity of the nearly full-length mitochondrial COI gene (1467 bp) was analyzed and compared among previously reported and newly collected individuals of the three host plant associations on a wide geographic range. Multiplex PCR was designed and evaluated for discriminating H. obsoletus host plant associations based on the size of amplified fragments: 1084 bp for the Cf association, 645 bp for the Ca–Ud association, and 355 bp for the Vac association. Examples of the epidemiological value of combining data on the genetic characteristics of the vector and the pathogen are provided. The method is intended to facilitate an accurate identification of the vector’s phylogenetic lineage, natural host plant preference, and epidemiological transmission routes of ‘Ca. P. solani’. When applied to H. obsoletus specimens collected from cultivated plants within an agroecosystem and combined with ‘Ca. P. solani’ genotyping, the method should provide valuable information on disease epidemiology, source(s) of emergence, and transmission routes.",
publisher = "MDPI, Basel",
journal = "AGRONOMY-BASEL",
title = "Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of 'Ca. Phytoplasma solani' Epidemiology",
number = "3",
volume = "13",
doi = "10.3390/agronomy13030831"
}

Jovic, J.,& Tosevski, I.. (2023). Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of 'Ca. Phytoplasma solani' Epidemiology. in AGRONOMY-BASEL
MDPI, Basel., 13(3).
https://doi.org/10.3390/agronomy13030831

Jovic J, Tosevski I. Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of 'Ca. Phytoplasma solani' Epidemiology. in AGRONOMY-BASEL. 2023;13(3).
doi:10.3390/agronomy13030831 .

Jovic, Jelena, Tosevski, Ivo, "Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of 'Ca. Phytoplasma solani' Epidemiology" in AGRONOMY-BASEL, 13, no. 3 (2023),
https://doi.org/10.3390/agronomy13030831 . .

TY  - JOUR
AU  - Tosevski, Ivo
AU  - Sing, Sharlene
AU  - Caldara, Roberto
AU  - Weaver, David
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Hinz, Hariet
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/709
AB  - A seed-feeding weevil introduced to North America (NA) as a biological control agent of the invasive toadflax Linaria dalmatica (L.) Mill., identified then as Gymnetron antirrhini “Dalmatian host race” and subsequently confirmed as established, was revealed through our study to be a separate species, i.e., Rhinusa dieckmanni (Behne) (Coleoptera: Curculionidae). This weevil species was presumed to be endemic in its native range, with a distribution restricted to Mount Rila in southwestern Bulgaria. We conducted a comprehensive study of seed-feeding weevils associated with L. dalmatica, L. dalmatica ssp. macedonica (Griseb.) D.A. Sutton, L. genistifolia (L.) Mill., and L. grandiflora Desf. across a broad geographic area of their native range. Those results revealed that all four host plants were used by R. dieckmanni and thus the native geographic range of the species is wider than expected, encompassing the Balkans and the Anatolian Plateau. Our observations suggest that phenotypes of this weevil are highly variable and dependent on the seed capsule size of the Linaria host population. The haplotype network based on mitochondrial COII, 16S genes, and nuclear EF 1-α gene genealogy confirmed the conspecific nature of geographically distant weevil populations, that is, R. dieckmanni phenotypes utilizing L. genistifolia, L. dalmatica, and L. grandiflora for larval development. Specimens collected from L. dalmatica in the northwestern USA shared the same haplotypes as samples from L. dalmatica ssp. macedonica in southwestern North Macedonia, supporting the known introduction history of the North American population. Females from these populations have relatively short rostrums, which may limit their reproductive success on North American invasive L. dalmatica with larger seed capsules.
PB  - Elsevier
T2  - Biological Control
T1  - Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America
VL  - 183
DO  - 10.1016/j.biocontrol.2023.105270
ER  - 

@article{
author = "Tosevski, Ivo and Sing, Sharlene and Caldara, Roberto and Weaver, David and Jovic, Jelena and Krstić, Oliver and Hinz, Hariet",
year = "2023",
abstract = "A seed-feeding weevil introduced to North America (NA) as a biological control agent of the invasive toadflax Linaria dalmatica (L.) Mill., identified then as Gymnetron antirrhini “Dalmatian host race” and subsequently confirmed as established, was revealed through our study to be a separate species, i.e., Rhinusa dieckmanni (Behne) (Coleoptera: Curculionidae). This weevil species was presumed to be endemic in its native range, with a distribution restricted to Mount Rila in southwestern Bulgaria. We conducted a comprehensive study of seed-feeding weevils associated with L. dalmatica, L. dalmatica ssp. macedonica (Griseb.) D.A. Sutton, L. genistifolia (L.) Mill., and L. grandiflora Desf. across a broad geographic area of their native range. Those results revealed that all four host plants were used by R. dieckmanni and thus the native geographic range of the species is wider than expected, encompassing the Balkans and the Anatolian Plateau. Our observations suggest that phenotypes of this weevil are highly variable and dependent on the seed capsule size of the Linaria host population. The haplotype network based on mitochondrial COII, 16S genes, and nuclear EF 1-α gene genealogy confirmed the conspecific nature of geographically distant weevil populations, that is, R. dieckmanni phenotypes utilizing L. genistifolia, L. dalmatica, and L. grandiflora for larval development. Specimens collected from L. dalmatica in the northwestern USA shared the same haplotypes as samples from L. dalmatica ssp. macedonica in southwestern North Macedonia, supporting the known introduction history of the North American population. Females from these populations have relatively short rostrums, which may limit their reproductive success on North American invasive L. dalmatica with larger seed capsules.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America",
volume = "183",
doi = "10.1016/j.biocontrol.2023.105270"
}

Tosevski, I., Sing, S., Caldara, R., Weaver, D., Jovic, J., Krstić, O.,& Hinz, H.. (2023). Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America. in Biological Control
Elsevier., 183.
https://doi.org/10.1016/j.biocontrol.2023.105270

Tosevski I, Sing S, Caldara R, Weaver D, Jovic J, Krstić O, Hinz H. Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America. in Biological Control. 2023;183.
doi:10.1016/j.biocontrol.2023.105270 .

Tosevski, Ivo, Sing, Sharlene, Caldara, Roberto, Weaver, David, Jovic, Jelena, Krstić, Oliver, Hinz, Hariet, "Retrospective use of integrative taxonomy in classical biological control: The unintentional introduction of the weevil Rhinusa dieckmanni to North America" in Biological Control, 183 (2023),
https://doi.org/10.1016/j.biocontrol.2023.105270 . .

TY  - JOUR
AU  - Radonjić, Sanja
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Hrnčić, Snježana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/691
AB  - Abstract Flavescence dorée (FD) is a severe and epidemic grapevine yellows disease, affecting most of the major European viticultural regions and classified as a quarantine pest. It is transmitted primarily by the ampelophagous Neartic deltocephalinae leafhopper, Scaphoideus titanus. The causative agent of the disease is FD phytoplasma (FDp), Vectotypes II and III, member of the 16S rRNA taxonomic subgroups V-C and V-D. Recent evidence indicates that FDp is native to Europe. Since 2018, vineyards in Montenegro have been subject to a preventive surveillance for the presence of FDp. In July of 2021, 56 samples of symptomatic grapevines were collected from vineyards in the major wine-growing regions. Using conventional nested PCR on the 16SrV group-specific FD9 marker (secY gene) and PCR-RFLP analysis of the 16S gene, the 16SrV-C phytoplasma subgroup was identified in a single grapevine from the Godinje location of the Crmnica wine subregion. The isolate was identified as Vectotype III, Map-FD3/VmpA-III genotype M51/III-c11 after characterizing the epidemiologicaly informative map and vmpA genes. In October of the same year, additional grapevines and potential reservoir or natural host plants of FDp and related phytoplasmas were sampled in the Godinje location. No new FDp-infected grapevines were found, indicating that this was likely an incidental transmission by natural, indigenous vectors and not an outbreak. However, one clematis and six alders were found carrying 16SrV group phytoplasmas and were subjected to map/vmpA typing, along with isolates of previously collected clematis plants in the Nudo wine region, and alder trees in non-viticultural areas and the potential wine region of Montenegrin North. The clematis plant in Godinje shared the same FDp Vectotype III as the infected vine, suggesting that clematis was the likely source of infection. On the other hand, alders in Godinje harbored VmpA-II cluster genotypes of either Map-FD2 M148 or AldY (alder yellows) M52, or multiple map gene sequences. Finally, all three FDp clusters of epidemic genotypes were found in clematis and alder reservoir plants in various regions of Montenegro. According to Map/VmpA Vectotype classification, clematis was identified as a potential source of FD3/III-c, and alders of FD1/III-f and FD2/II-d cluster genotypes. These findings call for stringent management and monitoring of S. titanus in Montenegrin vineyards to prevent FDp epidemic outbreaks.
PB  - Springer Verlag
T2  - JOURNAL OF PLANT PATHOLOGY
T1  - The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs
EP  - 427
IS  - 2
SP  - 419
VL  - 105
DO  - 10.1007/s42161-023-01318-z
ER  - 

@article{
author = "Radonjić, Sanja and Krstić, Oliver and Cvrković, Tatjana and Hrnčić, Snježana and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo and Jovic, Jelena",
year = "2023",
abstract = "Abstract Flavescence dorée (FD) is a severe and epidemic grapevine yellows disease, affecting most of the major European viticultural regions and classified as a quarantine pest. It is transmitted primarily by the ampelophagous Neartic deltocephalinae leafhopper, Scaphoideus titanus. The causative agent of the disease is FD phytoplasma (FDp), Vectotypes II and III, member of the 16S rRNA taxonomic subgroups V-C and V-D. Recent evidence indicates that FDp is native to Europe. Since 2018, vineyards in Montenegro have been subject to a preventive surveillance for the presence of FDp. In July of 2021, 56 samples of symptomatic grapevines were collected from vineyards in the major wine-growing regions. Using conventional nested PCR on the 16SrV group-specific FD9 marker (secY gene) and PCR-RFLP analysis of the 16S gene, the 16SrV-C phytoplasma subgroup was identified in a single grapevine from the Godinje location of the Crmnica wine subregion. The isolate was identified as Vectotype III, Map-FD3/VmpA-III genotype M51/III-c11 after characterizing the epidemiologicaly informative map and vmpA genes. In October of the same year, additional grapevines and potential reservoir or natural host plants of FDp and related phytoplasmas were sampled in the Godinje location. No new FDp-infected grapevines were found, indicating that this was likely an incidental transmission by natural, indigenous vectors and not an outbreak. However, one clematis and six alders were found carrying 16SrV group phytoplasmas and were subjected to map/vmpA typing, along with isolates of previously collected clematis plants in the Nudo wine region, and alder trees in non-viticultural areas and the potential wine region of Montenegrin North. The clematis plant in Godinje shared the same FDp Vectotype III as the infected vine, suggesting that clematis was the likely source of infection. On the other hand, alders in Godinje harbored VmpA-II cluster genotypes of either Map-FD2 M148 or AldY (alder yellows) M52, or multiple map gene sequences. Finally, all three FDp clusters of epidemic genotypes were found in clematis and alder reservoir plants in various regions of Montenegro. According to Map/VmpA Vectotype classification, clematis was identified as a potential source of FD3/III-c, and alders of FD1/III-f and FD2/II-d cluster genotypes. These findings call for stringent management and monitoring of S. titanus in Montenegrin vineyards to prevent FDp epidemic outbreaks.",
publisher = "Springer Verlag",
journal = "JOURNAL OF PLANT PATHOLOGY",
title = "The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs",
pages = "427-419",
number = "2",
volume = "105",
doi = "10.1007/s42161-023-01318-z"
}

Radonjić, S., Krstić, O., Cvrković, T., Hrnčić, S., Marinković, S., Mitrović, M., Tosevski, I.,& Jovic, J.. (2023). The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs. in JOURNAL OF PLANT PATHOLOGY
Springer Verlag., 105(2), 419-427.
https://doi.org/10.1007/s42161-023-01318-z

Radonjić S, Krstić O, Cvrković T, Hrnčić S, Marinković S, Mitrović M, Tosevski I, Jovic J. The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs. in JOURNAL OF PLANT PATHOLOGY. 2023;105(2):419-427.
doi:10.1007/s42161-023-01318-z .

Radonjić, Sanja, Krstić, Oliver, Cvrković, Tatjana, Hrnčić, Snježana, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, Jovic, Jelena, "The first report on the occurrence of Flavescence doree phytoplasma affecting grapevine in vineyards of Montenegro and an overview of epidemic genotypes in natural plant reservoirs" in JOURNAL OF PLANT PATHOLOGY, 105, no. 2 (2023):419-427,
https://doi.org/10.1007/s42161-023-01318-z . .

TY  - CONF
AU  - Serga, S.
AU  - Lavrinienko, A.
AU  - Tyukmaeva, V.
AU  - Kesäniemi, J.
AU  - Máca, J.
AU  - Oboňa, J.
AU  - Krstić, Oliver
AU  - Toševski, Ivo
AU  - Phooi, C.L.
AU  - Lapegue, M.
AU  - Estoup, A.
AU  - Loiseau, A.
AU  - Deschamps, C.
AU  - Gautier, M.
AU  - Knott, E.
AU  - Kozeretska, I.
AU  - Rode, N.O.
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1284
AB  - Understanding the factors that drive the prevalence of endosymbionts in natural populations is a
central goal of evolutionary ecology. The success of maternally transmitted symbionts can be
explained by three parameters: reproductive manipulations of the host, vertical transmission
rates and effects on host fitness. Those parameters can be modulated by both environmental
and genetic factors. The Wolbachia strain wSuz, which infects the invasive pest species
Drosophila suzukii, is a canonical example of maternally transmitted symbiont with low to
intermediate prevalence in host populations and weak reproductive manipulation. For Wolbachia
strains with such properties, the mechanisms inherent to the infection dynamics remain poorly
understood. We investigated infection frequencies and wSuz intra-strain polymorphism in 24
natural populations of D. suzukii from both native (China and Japan) and invasive areas (Europe
and America). Infection frequencies in populations from China were on average significantly
higher than those in populations from invasive areas. Based on the study of an insertion
sequence site and a genomic rearrangement polymorphism in wSuz genome, we could identify
three wSuz variants (i.e. European, American and Asian) corresponding to the initial sample
location. More in-depth investigations indicated that the European variant was also present in
some Asian populations and that one population from Europe included the European and
American variants. Additional analyses based on whole-genome sequencing of 70 D. suzukii
population samples showed lower Wolbachia genomic diversity in invasive populations
(America and Europe) than in native (Japan, Korea and China) populations, consistent with a
bottleneck in invasive populations. Finally, we analyzed two types of factors that could affect
Wolbachia infection frequencies in natural populations: climatic variation and
Wolbachia-induced cytoplasmic incompatibility. We found that variations in the strength of
cytoplasmic incompatibilities or in monthly temperatures were not sufficient to fully explain
observed prevalence pattern. Overall, our results show that, despite reduced intra-strain
genomic diversity, both population prevalence and phenotypic effects on host reproduction can
vary greatly among Wolbachia variants, suggesting complex interactions with host genetic
background and environmental factors.
PB  - Orthodox Academy of Greece
C3  - 11th Wolbachia conference, Crete, 11-16 VI 2023
T1  - Factors driving Wolbachia prevalence in native and invasive populations of Drosophila suzukii
EP  - 17
SP  - 16
ER  - 

@conference{
author = "Serga, S. and Lavrinienko, A. and Tyukmaeva, V. and Kesäniemi, J. and Máca, J. and Oboňa, J. and Krstić, Oliver and Toševski, Ivo and Phooi, C.L. and Lapegue, M. and Estoup, A. and Loiseau, A. and Deschamps, C. and Gautier, M. and Knott, E. and Kozeretska, I. and Rode, N.O.",
year = "2023",
abstract = "Understanding the factors that drive the prevalence of endosymbionts in natural populations is a
central goal of evolutionary ecology. The success of maternally transmitted symbionts can be
explained by three parameters: reproductive manipulations of the host, vertical transmission
rates and effects on host fitness. Those parameters can be modulated by both environmental
and genetic factors. The Wolbachia strain wSuz, which infects the invasive pest species
Drosophila suzukii, is a canonical example of maternally transmitted symbiont with low to
intermediate prevalence in host populations and weak reproductive manipulation. For Wolbachia
strains with such properties, the mechanisms inherent to the infection dynamics remain poorly
understood. We investigated infection frequencies and wSuz intra-strain polymorphism in 24
natural populations of D. suzukii from both native (China and Japan) and invasive areas (Europe
and America). Infection frequencies in populations from China were on average significantly
higher than those in populations from invasive areas. Based on the study of an insertion
sequence site and a genomic rearrangement polymorphism in wSuz genome, we could identify
three wSuz variants (i.e. European, American and Asian) corresponding to the initial sample
location. More in-depth investigations indicated that the European variant was also present in
some Asian populations and that one population from Europe included the European and
American variants. Additional analyses based on whole-genome sequencing of 70 D. suzukii
population samples showed lower Wolbachia genomic diversity in invasive populations
(America and Europe) than in native (Japan, Korea and China) populations, consistent with a
bottleneck in invasive populations. Finally, we analyzed two types of factors that could affect
Wolbachia infection frequencies in natural populations: climatic variation and
Wolbachia-induced cytoplasmic incompatibility. We found that variations in the strength of
cytoplasmic incompatibilities or in monthly temperatures were not sufficient to fully explain
observed prevalence pattern. Overall, our results show that, despite reduced intra-strain
genomic diversity, both population prevalence and phenotypic effects on host reproduction can
vary greatly among Wolbachia variants, suggesting complex interactions with host genetic
background and environmental factors.",
publisher = "Orthodox Academy of Greece",
journal = "11th Wolbachia conference, Crete, 11-16 VI 2023",
title = "Factors driving Wolbachia prevalence in native and invasive populations of Drosophila suzukii",
pages = "17-16"
}

Serga, S., Lavrinienko, A., Tyukmaeva, V., Kesäniemi, J., Máca, J., Oboňa, J., Krstić, O., Toševski, I., Phooi, C.L., Lapegue, M., Estoup, A., Loiseau, A., Deschamps, C., Gautier, M., Knott, E., Kozeretska, I.,& Rode, N.O.. (2023). Factors driving Wolbachia prevalence in native and invasive populations of Drosophila suzukii. in 11th Wolbachia conference, Crete, 11-16 VI 2023
Orthodox Academy of Greece., 16-17.

Serga S, Lavrinienko A, Tyukmaeva V, Kesäniemi J, Máca J, Oboňa J, Krstić O, Toševski I, Phooi C, Lapegue M, Estoup A, Loiseau A, Deschamps C, Gautier M, Knott E, Kozeretska I, Rode N. Factors driving Wolbachia prevalence in native and invasive populations of Drosophila suzukii. in 11th Wolbachia conference, Crete, 11-16 VI 2023. 2023;:16-17..

Serga, S., Lavrinienko, A., Tyukmaeva, V., Kesäniemi, J., Máca, J., Oboňa, J., Krstić, Oliver, Toševski, Ivo, Phooi, C.L., Lapegue, M., Estoup, A., Loiseau, A., Deschamps, C., Gautier, M., Knott, E., Kozeretska, I., Rode, N.O., "Factors driving Wolbachia prevalence in native and invasive populations of Drosophila suzukii" in 11th Wolbachia conference, Crete, 11-16 VI 2023 (2023):16-17.

TY  - CONF
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Jakovljević, Miljana
AU  - Marinković, Slavica
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/815
PB  - Entomološko društvo Srbije
C3  - XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16
T1  - Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze
ER  - 

@conference{
author = "Jovic, Jelena and Krstić, Oliver and Cvrković, Tatjana and Jakovljević, Miljana and Marinković, Slavica and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
publisher = "Entomološko društvo Srbije",
journal = "XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16",
title = "Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze"
}

Jovic, J., Krstić, O., Cvrković, T., Jakovljević, M., Marinković, S., Mitrović, M.,& Tosevski, I.. (2022). Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze. in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16
Entomološko društvo Srbije..

Jovic J, Krstić O, Cvrković T, Jakovljević M, Marinković S, Mitrović M, Tosevski I. Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze. in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16. 2022;..

Jovic, Jelena, Krstić, Oliver, Cvrković, Tatjana, Jakovljević, Miljana, Marinković, Slavica, Mitrović, Milana, Tosevski, Ivo, "Uticaj alohtonih cikada (Hemiptera: Auchenorrhyncha) na epidemiju i epidemiologiju Flavescence dorée fitoplazme vinove loze" in XIII Symposium of entomologists of Serbia with international parcipation  2022 Sep 14-16 (2022).

TY  - CONF
AU  - Jakovljević, Miljana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
AU  - Mitrović, Milana
AU  - Marinković, Slavica
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/794
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16
T1  - Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri
ER  - 

@conference{
author = "Jakovljević, Miljana and Tosevski, Ivo and Jovic, Jelena and Mitrović, Milana and Marinković, Slavica and Krstić, Oliver and Cvrković, Tatjana",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16",
title = "Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri"
}

Jakovljević, M., Tosevski, I., Jovic, J., Mitrović, M., Marinković, S., Krstić, O.,& Cvrković, T.. (2022). Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri. in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16
Entomological Society of Serbia..

Jakovljević M, Tosevski I, Jovic J, Mitrović M, Marinković S, Krstić O, Cvrković T. Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri. in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16. 2022;..

Jakovljević, Miljana, Tosevski, Ivo, Jovic, Jelena, Mitrović, Milana, Marinković, Slavica, Krstić, Oliver, Cvrković, Tatjana, "Kopulatorne strukture mužjaka vrsta roda Euscelis (Cicadellidae: Deltocephalinae) kao taksonomski karakteri" in XIII Symposium of entomologists of Serbia with international partipation Pirot, 2022 Sep 14-16 (2022).

TY  - CONF
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/786
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot
T1  - Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme
ER  - 

@conference{
author = "Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot",
title = "Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme"
}

Cvrković, T., Jovic, J., Krstić, O., Marinković, S., Jakovljević, M., Mitrović, M.,& Tosevski, I.. (2022). Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme. in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot
Entomological Society of Serbia..

Cvrković T, Jovic J, Krstić O, Marinković S, Jakovljević M, Mitrović M, Tosevski I. Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme. in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot. 2022;..

Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, "Vektorska uloga vrste Dictyophara europaea (Hemiptera: Dictyopharidae) u prenošenju stolbur fitoplazme" in XIII Symposium of entomologists of Serbia with international participation, Sep 14-16; 2022 Pirot (2022).

TY  - CONF
AU  - Marinković, Slavica
AU  - Krstić, Oliver
AU  - Jakovljević, Miljana
AU  - Cvrković, Tatjana
AU  - Mitrović, Milana
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/777
PB  - Entomological Society of Serbia
C3  - XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.
T1  - Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji
ER  - 

@conference{
author = "Marinković, Slavica and Krstić, Oliver and Jakovljević, Miljana and Cvrković, Tatjana and Mitrović, Milana and Jovic, Jelena and Tosevski, Ivo",
year = "2022",
publisher = "Entomological Society of Serbia",
journal = "XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.",
title = "Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji"
}

Marinković, S., Krstić, O., Jakovljević, M., Cvrković, T., Mitrović, M., Jovic, J.,& Tosevski, I.. (2022). Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji. in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.
Entomological Society of Serbia..

Marinković S, Krstić O, Jakovljević M, Cvrković T, Mitrović M, Jovic J, Tosevski I. Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji. in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022.. 2022;..

Marinković, Slavica, Krstić, Oliver, Jakovljević, Miljana, Cvrković, Tatjana, Mitrović, Milana, Jovic, Jelena, Tosevski, Ivo, "Genetička struktura populacija tripsa Frankliniella occidentalis (Thysanoptera: Thripidae) u Srbiji" in XIII Symposium of entomologists of Serbia with international parcipation, Pirot , 14-16. 09. 2022. (2022).

TY  - JOUR
AU  - Krstić, Oliver
AU  - Cvrković, Tatjana
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
AU  - Jovic, Jelena
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/719
AB  - Flavescence dorée (FD) is a European quarantine disease of grapevine caused by FD phytoplasma (FDp) transmitted by the leafhopper of North American origin Scaphoideus titanus. The disease affects the most important viticultural regions of Europe and all wine-growing regions of Serbia. Unlike the insect vector, the pathogen is native to Europe and associated with several wild host plants among which alder trees as the main source of two out of three map genetic clusters of pathogen variants (Map-FD1 and FD2). Heretofore, the FDp epidemic in Serbian vineyards was thought to be monotypic, i.e., caused by the single genotype of the Map-FD3 cluster, M51, and correlated with clematis as the natural source plant. This study aimed to provide data on genetic diversity, through map and vmpA gene typing, and insights into ecological properties of epidemiological cycles driving the epidemic outbreaks of FD in Serbia today. Map genotyping of 270 grapevine isolates collected from 2017 to 2019 confirmed M51 as autochthonous genotype widespread in all wine producing regions of Serbia and the dominant FDp epidemic genotype in most of the districts (75%, 202/270 isolates), except in north Serbia where multiple outbreaks of M12 Map-FD3 were recorded (54 isolates). Tree of heaven is reported as a new FDp plant reservoir for the Serbian vineyards, hosting the M51 genotype, along with clematis. An outbreak of a new endemic Map-FD3 genotype M144 was documented in grapevine samples from east Serbia (5 isolates), correlating with previous findings of the same genotype in clematis. In addition, single grapevine infections with five new Map-FD3 genotypes (M150-M154) were recorded in central Serbia, thus indicating high endemic potential for new outbreaks. The vmpA typing placed all Map-FD3 isolates into the VmpA-III cluster, i.e., Vectotype III. Finally, we found direct evidence that at least two FDp endemic genotypes, M89 and M148, of the Map-FD2/VmpA-II have escaped from alders and propagated in the grapevine-S. titanus pathosystem in Serbia (Vectotype II). Our findings confirm the high complexity of the FDp ecological cycle and provide evidence of a unique, autochthonous Balkan epidemiology sourced endemically.
PB  - MDPI Basel
T2  - Agronomy
T1  - Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes
IS  - 2
VL  - 12
DO  - 10.3390/agronomy12020448
ER  - 

@article{
author = "Krstić, Oliver and Cvrković, Tatjana and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo and Jovic, Jelena",
year = "2022",
abstract = "Flavescence dorée (FD) is a European quarantine disease of grapevine caused by FD phytoplasma (FDp) transmitted by the leafhopper of North American origin Scaphoideus titanus. The disease affects the most important viticultural regions of Europe and all wine-growing regions of Serbia. Unlike the insect vector, the pathogen is native to Europe and associated with several wild host plants among which alder trees as the main source of two out of three map genetic clusters of pathogen variants (Map-FD1 and FD2). Heretofore, the FDp epidemic in Serbian vineyards was thought to be monotypic, i.e., caused by the single genotype of the Map-FD3 cluster, M51, and correlated with clematis as the natural source plant. This study aimed to provide data on genetic diversity, through map and vmpA gene typing, and insights into ecological properties of epidemiological cycles driving the epidemic outbreaks of FD in Serbia today. Map genotyping of 270 grapevine isolates collected from 2017 to 2019 confirmed M51 as autochthonous genotype widespread in all wine producing regions of Serbia and the dominant FDp epidemic genotype in most of the districts (75%, 202/270 isolates), except in north Serbia where multiple outbreaks of M12 Map-FD3 were recorded (54 isolates). Tree of heaven is reported as a new FDp plant reservoir for the Serbian vineyards, hosting the M51 genotype, along with clematis. An outbreak of a new endemic Map-FD3 genotype M144 was documented in grapevine samples from east Serbia (5 isolates), correlating with previous findings of the same genotype in clematis. In addition, single grapevine infections with five new Map-FD3 genotypes (M150-M154) were recorded in central Serbia, thus indicating high endemic potential for new outbreaks. The vmpA typing placed all Map-FD3 isolates into the VmpA-III cluster, i.e., Vectotype III. Finally, we found direct evidence that at least two FDp endemic genotypes, M89 and M148, of the Map-FD2/VmpA-II have escaped from alders and propagated in the grapevine-S. titanus pathosystem in Serbia (Vectotype II). Our findings confirm the high complexity of the FDp ecological cycle and provide evidence of a unique, autochthonous Balkan epidemiology sourced endemically.",
publisher = "MDPI Basel",
journal = "Agronomy",
title = "Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes",
number = "2",
volume = "12",
doi = "10.3390/agronomy12020448"
}

Krstić, O., Cvrković, T., Marinković, S., Jakovljević, M., Mitrović, M., Tosevski, I.,& Jovic, J.. (2022). Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes. in Agronomy
MDPI Basel., 12(2).
https://doi.org/10.3390/agronomy12020448

Krstić O, Cvrković T, Marinković S, Jakovljević M, Mitrović M, Tosevski I, Jovic J. Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes. in Agronomy. 2022;12(2).
doi:10.3390/agronomy12020448 .

Krstić, Oliver, Cvrković, Tatjana, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, Jovic, Jelena, "Genetic Diversity of Flavescence Dorée Phytoplasmas in Vineyards of Serbia: From the Widespread Occurrence of Autochthonous Map-M51 to the Emergence of Endemic Map-FD2 (Vectotype II) and New Map-FD3 (Vectotype III) Epidemic Genotypes" in Agronomy, 12, no. 2 (2022),
https://doi.org/10.3390/agronomy12020448 . .

TY  - JOUR
AU  - Cvrković, Tatjana
AU  - Jovic, Jelena
AU  - Krstić, Oliver
AU  - Marinković, Slavica
AU  - Jakovljević, Miljana
AU  - Mitrović, Milana
AU  - Tosevski, Ivo
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/718
AB  - Bois noir, an economically important disease of grapevine yellows that causes significant economic losses in wine production, is associated with ‘Candidatus Phytoplasma solani’ and transmitted to grapevines by cixiids Hyalesthes obsoletus and Reptalus panzeri. Polyphagous planthopper Dictyophara europaea, commonly found in natural habitats, harbors phytoplasmas from distinct groups and is an alternative vector in the open epidemiological cycles of the Flavescence dorée phytoplasma in grapevine in European vineyards. This study addresses the role of D. europaea in the transmission cycle(s) of ‘Ca. P. solani’ among wild habitats, natural reservoir plants, and the vineyard agroecosystem using MLSA and transmission trials with naturally infected adults to grapevine and Catharanthus roseus. The infection rates of D. europaea ranged from 7% to 13% in diverse locations, while reservoir herbaceous plants were infected in the amount of 29%. A total of 13 CaPsol MLSA genotypes were detected in D. europaea (7) and plants (8). Nine of them corresponded to previously identified genotypes. Two new genotypes were found in D. europaea (tuf-b1/S1/V14/Rqg50-sv1 and tuf-b1/S18/V14/Rqg50-sv1) and one in Convolvulus arvensis (tuf-b1/S1/V2-TA/Rqg31-sv1), whereas one was shared by two hosts, Crepis foetida and Daucus carota (tuf-b1/S1/V2-TA/STOL-sv1). Naturally infected D. europaea successfully transmitted the tuf-b1/S1/V2-TA/STOL type to five grapevines and six periwinkles, tuf-b1/S1/V2-TA/Rqg31 to one grapevine, and tuf-b1/S1/V2-TA/Rqg50 to one periwinkle, indicating that D. europaea is an intermediate vector in CaPsol epidemiological cycles.
PB  - MDPI Basel
T2  - Horticulturae
T1  - Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’
IS  - 7
SP  - 654
VL  - 8
DO  - 10.3390/horticulturae8070654
ER  - 

@article{
author = "Cvrković, Tatjana and Jovic, Jelena and Krstić, Oliver and Marinković, Slavica and Jakovljević, Miljana and Mitrović, Milana and Tosevski, Ivo",
year = "2022",
abstract = "Bois noir, an economically important disease of grapevine yellows that causes significant economic losses in wine production, is associated with ‘Candidatus Phytoplasma solani’ and transmitted to grapevines by cixiids Hyalesthes obsoletus and Reptalus panzeri. Polyphagous planthopper Dictyophara europaea, commonly found in natural habitats, harbors phytoplasmas from distinct groups and is an alternative vector in the open epidemiological cycles of the Flavescence dorée phytoplasma in grapevine in European vineyards. This study addresses the role of D. europaea in the transmission cycle(s) of ‘Ca. P. solani’ among wild habitats, natural reservoir plants, and the vineyard agroecosystem using MLSA and transmission trials with naturally infected adults to grapevine and Catharanthus roseus. The infection rates of D. europaea ranged from 7% to 13% in diverse locations, while reservoir herbaceous plants were infected in the amount of 29%. A total of 13 CaPsol MLSA genotypes were detected in D. europaea (7) and plants (8). Nine of them corresponded to previously identified genotypes. Two new genotypes were found in D. europaea (tuf-b1/S1/V14/Rqg50-sv1 and tuf-b1/S18/V14/Rqg50-sv1) and one in Convolvulus arvensis (tuf-b1/S1/V2-TA/Rqg31-sv1), whereas one was shared by two hosts, Crepis foetida and Daucus carota (tuf-b1/S1/V2-TA/STOL-sv1). Naturally infected D. europaea successfully transmitted the tuf-b1/S1/V2-TA/STOL type to five grapevines and six periwinkles, tuf-b1/S1/V2-TA/Rqg31 to one grapevine, and tuf-b1/S1/V2-TA/Rqg50 to one periwinkle, indicating that D. europaea is an intermediate vector in CaPsol epidemiological cycles.",
publisher = "MDPI Basel",
journal = "Horticulturae",
title = "Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’",
number = "7",
pages = "654",
volume = "8",
doi = "10.3390/horticulturae8070654"
}

Cvrković, T., Jovic, J., Krstić, O., Marinković, S., Jakovljević, M., Mitrović, M.,& Tosevski, I.. (2022). Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’. in Horticulturae
MDPI Basel., 8(7), 654.
https://doi.org/10.3390/horticulturae8070654

Cvrković T, Jovic J, Krstić O, Marinković S, Jakovljević M, Mitrović M, Tosevski I. Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’. in Horticulturae. 2022;8(7):654.
doi:10.3390/horticulturae8070654 .

Cvrković, Tatjana, Jovic, Jelena, Krstić, Oliver, Marinković, Slavica, Jakovljević, Miljana, Mitrović, Milana, Tosevski, Ivo, "Epidemiological Role of Dictyophara europaea (Hemiptera: Dictyopharidae) in the Transmission of ‘Candidatus Phytoplasma solani’" in Horticulturae, 8, no. 7 (2022):654,
https://doi.org/10.3390/horticulturae8070654 . .

TY  - JOUR
AU  - Skuhrovec, Jiri
AU  - Gosik, Rafał
AU  - Caldara, Roberto
AU  - Tosevski, Ivo
AU  - Batyra, Aleksandra
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/694
AB  - The immature stages of the following five Palaearctic Gymnetron species are described for the first time: G. tibiellum Desbrochers des Loges, 1900, G. veronicae (Germar, 1821), G. rotundicolle Gyllenhal, 1838, G. melanarium (Germar, 1821), and G. villosulum Gyllenhal, 1838. These species belong to four different groups previously established according to a phylogenetic analysis: the first two belong to the G. veronicae group and the other three to groups respectively bearing their name (G. rotundicolle, G. melanarium, and G. villosulum groups). All these species exhibit several diagnostic characters distinguishing them from each other. Some characters that can be used to separate Gymnetron from other genera in the tribe are also suggested. Three highly significant characters for the larvae and three for the pupae were identified. For the larvae they are: (1) labial palpi with single palpomeres, (2) all spiracles unicameral, and (3) epipharynx with a single pair of mes or none at all. For the pupae they are: (1) the pronotum with prominent pronotal protuberances, (2) abdominal segment VIII with a conical abdominal protuberance dorsally, and (3) very short or even reduced urogomphi. The species studied here are compared with those Gymnetron species already known and with other genera in the tribe Mecinini. Keys to the larvae and pupae described here are provided. All the characters used for identification are illustrated by photographs or drawings.
PB  - Pensoft Publishers
T2  - Zookeys
T1  - Description of immature stages of Gymnetron species (Coleoptera, Curculionidae, Curculioninae), with particular emphasis on the diagnostic morphological characters at the generic and specific levels
IS  - 45
VL  - 1090
DO  - 10.3897/zookeys.1090.78741
ER  - 

@article{
author = "Skuhrovec, Jiri and Gosik, Rafał and Caldara, Roberto and Tosevski, Ivo and Batyra, Aleksandra",
year = "2022",
abstract = "The immature stages of the following five Palaearctic Gymnetron species are described for the first time: G. tibiellum Desbrochers des Loges, 1900, G. veronicae (Germar, 1821), G. rotundicolle Gyllenhal, 1838, G. melanarium (Germar, 1821), and G. villosulum Gyllenhal, 1838. These species belong to four different groups previously established according to a phylogenetic analysis: the first two belong to the G. veronicae group and the other three to groups respectively bearing their name (G. rotundicolle, G. melanarium, and G. villosulum groups). All these species exhibit several diagnostic characters distinguishing them from each other. Some characters that can be used to separate Gymnetron from other genera in the tribe are also suggested. Three highly significant characters for the larvae and three for the pupae were identified. For the larvae they are: (1) labial palpi with single palpomeres, (2) all spiracles unicameral, and (3) epipharynx with a single pair of mes or none at all. For the pupae they are: (1) the pronotum with prominent pronotal protuberances, (2) abdominal segment VIII with a conical abdominal protuberance dorsally, and (3) very short or even reduced urogomphi. The species studied here are compared with those Gymnetron species already known and with other genera in the tribe Mecinini. Keys to the larvae and pupae described here are provided. All the characters used for identification are illustrated by photographs or drawings.",
publisher = "Pensoft Publishers",
journal = "Zookeys",
title = "Description of immature stages of Gymnetron species (Coleoptera, Curculionidae, Curculioninae), with particular emphasis on the diagnostic morphological characters at the generic and specific levels",
number = "45",
volume = "1090",
doi = "10.3897/zookeys.1090.78741"
}

Skuhrovec, J., Gosik, R., Caldara, R., Tosevski, I.,& Batyra, A.. (2022). Description of immature stages of Gymnetron species (Coleoptera, Curculionidae, Curculioninae), with particular emphasis on the diagnostic morphological characters at the generic and specific levels. in Zookeys
Pensoft Publishers., 1090(45).
https://doi.org/10.3897/zookeys.1090.78741

Skuhrovec J, Gosik R, Caldara R, Tosevski I, Batyra A. Description of immature stages of Gymnetron species (Coleoptera, Curculionidae, Curculioninae), with particular emphasis on the diagnostic morphological characters at the generic and specific levels. in Zookeys. 2022;1090(45).
doi:10.3897/zookeys.1090.78741 .

Skuhrovec, Jiri, Gosik, Rafał, Caldara, Roberto, Tosevski, Ivo, Batyra, Aleksandra, "Description of immature stages of Gymnetron species (Coleoptera, Curculionidae, Curculioninae), with particular emphasis on the diagnostic morphological characters at the generic and specific levels" in Zookeys, 1090, no. 45 (2022),
https://doi.org/10.3897/zookeys.1090.78741 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Zečević, Katarina
AU  - Delibašić, Goran
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
AU  - Krstić, Branka
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/692
AB  - Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confirmed the presence of GRSPaV. No amplification products were recorded in samples prepared from basal leaves or petioles. A serological assay confirmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the first report of GRSPaV present in Serbia. Further monitoring and increased phytosanitary control measures are needed to prevent potential spreading and introduction of new virus variants.
PB  - Springer
T2  - JOURNAL OF PLANT DISEASES AND PROTECTION
T1  - Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia
DO  - 10.1007/s41348-022-00656-x
ER  - 

@article{
author = "Stanković, Ivana and Zečević, Katarina and Delibašić, Goran and Jovic, Jelena and Tosevski, Ivo and Krstić, Branka",
year = "2022",
abstract = "Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confirmed the presence of GRSPaV. No amplification products were recorded in samples prepared from basal leaves or petioles. A serological assay confirmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the first report of GRSPaV present in Serbia. Further monitoring and increased phytosanitary control measures are needed to prevent potential spreading and introduction of new virus variants.",
publisher = "Springer",
journal = "JOURNAL OF PLANT DISEASES AND PROTECTION",
title = "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia",
doi = "10.1007/s41348-022-00656-x"
}

Stanković, I., Zečević, K., Delibašić, G., Jovic, J., Tosevski, I.,& Krstić, B.. (2022). Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in JOURNAL OF PLANT DISEASES AND PROTECTION
Springer..
https://doi.org/10.1007/s41348-022-00656-x

Stanković I, Zečević K, Delibašić G, Jovic J, Tosevski I, Krstić B. Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in JOURNAL OF PLANT DISEASES AND PROTECTION. 2022;.
doi:10.1007/s41348-022-00656-x .

Stanković, Ivana, Zečević, Katarina, Delibašić, Goran, Jovic, Jelena, Tosevski, Ivo, Krstić, Branka, "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia" in JOURNAL OF PLANT DISEASES AND PROTECTION (2022),
https://doi.org/10.1007/s41348-022-00656-x . .