TY  - JOUR
AU  - Mitrovski-Bogdanović, Ana
AU  - Mitrović, Milana
AU  - Ilić Milošević, Marijana
AU  - Žikić, Vladimir
AU  - Jamhour, Aiman
AU  - Ivanović, Ana
AU  - Tomanović, Željko
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/648
AB  - The main objective of the present paper was to analyse and compare the patterns of molecular and morphological divergence of European parasitoid wasps belonging to the diverse genus Aphidius Nees, 1818. The maximum likelihood and maximum parsimony trees constructed by including 64 different haplotypes of the barcoding region of mitochondrial cytochrome oxidase subunit I (mtCOI) identified for 33 Aphidius species showed identical topology. A high level (99%) of bootstrap support was found for the phylogenetic line consisting of A. ribis Haliday, 1834, A. chaetosiphonis Tomanović & Petrović, 2011 and A. hortensis Marshall, 1896, and for the group consisting of A. colemani Vierck, 1912, A. transcaspicus Telenga, 1958, A. asteris Haliday, 1834 and A. platensis Brethes, 1913. The remaining lineages on the trees were not significantly supported. We applied the approach of geometric morphometrics to explore morphological divergences in forewing size. A significant difference of mean wing shape was found between Aphidius species. The observed low resolution of the mtCOI gene of morphologically and ecologically well-defined Aphidius species is probably due to species hybridisation followed by introgression of mtDNA. Despite low resolution of the phylogenetic tree, the permutation test for a phylogenetic signal in wing shape was statistically significant, indicating that phylogenetically more closely related species are more similar than unrelated ones. A clear agreement between molecular and morphological variation was determined only for the two phylogenetically well-resolved groups.
PB  - Gesellschaft fuer Biologische Systematik
T2  - Organisms Diversity and Evolution
T1  - Molecular and morphological variation among the European species of the genus Aphidius Nees (Hymenoptera: Braconidae: Aphidiinae)
EP  - 436
SP  - 421
VL  - 21
DO  - 10.1007/s13127-021-00489-w
ER  - 

@article{
author = "Mitrovski-Bogdanović, Ana and Mitrović, Milana and Ilić Milošević, Marijana and Žikić, Vladimir and Jamhour, Aiman and Ivanović, Ana and Tomanović, Željko",
year = "2021",
abstract = "The main objective of the present paper was to analyse and compare the patterns of molecular and morphological divergence of European parasitoid wasps belonging to the diverse genus Aphidius Nees, 1818. The maximum likelihood and maximum parsimony trees constructed by including 64 different haplotypes of the barcoding region of mitochondrial cytochrome oxidase subunit I (mtCOI) identified for 33 Aphidius species showed identical topology. A high level (99%) of bootstrap support was found for the phylogenetic line consisting of A. ribis Haliday, 1834, A. chaetosiphonis Tomanović & Petrović, 2011 and A. hortensis Marshall, 1896, and for the group consisting of A. colemani Vierck, 1912, A. transcaspicus Telenga, 1958, A. asteris Haliday, 1834 and A. platensis Brethes, 1913. The remaining lineages on the trees were not significantly supported. We applied the approach of geometric morphometrics to explore morphological divergences in forewing size. A significant difference of mean wing shape was found between Aphidius species. The observed low resolution of the mtCOI gene of morphologically and ecologically well-defined Aphidius species is probably due to species hybridisation followed by introgression of mtDNA. Despite low resolution of the phylogenetic tree, the permutation test for a phylogenetic signal in wing shape was statistically significant, indicating that phylogenetically more closely related species are more similar than unrelated ones. A clear agreement between molecular and morphological variation was determined only for the two phylogenetically well-resolved groups.",
publisher = "Gesellschaft fuer Biologische Systematik",
journal = "Organisms Diversity and Evolution",
title = "Molecular and morphological variation among the European species of the genus Aphidius Nees (Hymenoptera: Braconidae: Aphidiinae)",
pages = "436-421",
volume = "21",
doi = "10.1007/s13127-021-00489-w"
}

Mitrovski-Bogdanović, A., Mitrović, M., Ilić Milošević, M., Žikić, V., Jamhour, A., Ivanović, A.,& Tomanović, Ž.. (2021). Molecular and morphological variation among the European species of the genus Aphidius Nees (Hymenoptera: Braconidae: Aphidiinae). in Organisms Diversity and Evolution
Gesellschaft fuer Biologische Systematik., 21, 421-436.
https://doi.org/10.1007/s13127-021-00489-w

Mitrovski-Bogdanović A, Mitrović M, Ilić Milošević M, Žikić V, Jamhour A, Ivanović A, Tomanović Ž. Molecular and morphological variation among the European species of the genus Aphidius Nees (Hymenoptera: Braconidae: Aphidiinae). in Organisms Diversity and Evolution. 2021;21:421-436.
doi:10.1007/s13127-021-00489-w .

Mitrovski-Bogdanović, Ana, Mitrović, Milana, Ilić Milošević, Marijana, Žikić, Vladimir, Jamhour, Aiman, Ivanović, Ana, Tomanović, Željko, "Molecular and morphological variation among the European species of the genus Aphidius Nees (Hymenoptera: Braconidae: Aphidiinae)" in Organisms Diversity and Evolution, 21 (2021):421-436,
https://doi.org/10.1007/s13127-021-00489-w . .

TY  - JOUR
AU  - Čkrkić, Jelisaveta
AU  - Petrovic, Andjelko
AU  - Kocić, Korana
AU  - Mitrović, Milana
AU  - Kavallieratos, Nickolas
AU  - Van Achterberg, Cornelis
AU  - Hebert, Paul
AU  - Tomanović, Željko
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/590
AB  - Abstract: Members of the Monoctonina subtribe have long been neglected in applied studies of
the subfamily Aphidiinae, due to their low economic importance, as they do not parasitize pests
of cultivated plants. Consequently, data about this group are scarce, including its taxonomy and
phylogeny. In the present study, we explore inter- and intraspecific genetic variation of Monoctonina
species, including genera Monoctonus Haliday 1833, Monoctonia Starý 1962, Falciconus Mackauer
1959 and Harkeria Cameron 1900. We employ two molecular markers, the barcode region of the
mitochondrial cytochrome c oxidase subunit I (COI) and the D2 region of the 28S nuclear gene
(28S rDNA), to analyze genetic structuring and phylogeny of all available Monoctonina species,
and combine them with morphological data for an integrative approach. We report one new species,
and three potentially new species which can be formally described when further specimens are
available. Analysis of phylogenetic relationships within the subtribe shows a basal position for the
genera Falciconus and Monoctonia, and the close relatedness of Harkeria and Monoctonus.
PB  - Basel : MDPI
T2  - Insects
T1  - Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)
IS  - 160
IS  - 3
VL  - 11
DO  - 10.3390/insects11030160
ER  - 

@article{
author = "Čkrkić, Jelisaveta and Petrovic, Andjelko and Kocić, Korana and Mitrović, Milana and Kavallieratos, Nickolas and Van Achterberg, Cornelis and Hebert, Paul and Tomanović, Željko",
year = "2020",
abstract = "Abstract: Members of the Monoctonina subtribe have long been neglected in applied studies of
the subfamily Aphidiinae, due to their low economic importance, as they do not parasitize pests
of cultivated plants. Consequently, data about this group are scarce, including its taxonomy and
phylogeny. In the present study, we explore inter- and intraspecific genetic variation of Monoctonina
species, including genera Monoctonus Haliday 1833, Monoctonia Starý 1962, Falciconus Mackauer
1959 and Harkeria Cameron 1900. We employ two molecular markers, the barcode region of the
mitochondrial cytochrome c oxidase subunit I (COI) and the D2 region of the 28S nuclear gene
(28S rDNA), to analyze genetic structuring and phylogeny of all available Monoctonina species,
and combine them with morphological data for an integrative approach. We report one new species,
and three potentially new species which can be formally described when further specimens are
available. Analysis of phylogenetic relationships within the subtribe shows a basal position for the
genera Falciconus and Monoctonia, and the close relatedness of Harkeria and Monoctonus.",
publisher = "Basel : MDPI",
journal = "Insects",
title = "Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)",
number = "160, 3",
volume = "11",
doi = "10.3390/insects11030160"
}

Čkrkić, J., Petrovic, A., Kocić, K., Mitrović, M., Kavallieratos, N., Van Achterberg, C., Hebert, P.,& Tomanović, Ž.. (2020). Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae). in Insects
Basel : MDPI., 11(160).
https://doi.org/10.3390/insects11030160

Čkrkić J, Petrovic A, Kocić K, Mitrović M, Kavallieratos N, Van Achterberg C, Hebert P, Tomanović Ž. Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae). in Insects. 2020;11(160).
doi:10.3390/insects11030160 .

Čkrkić, Jelisaveta, Petrovic, Andjelko, Kocić, Korana, Mitrović, Milana, Kavallieratos, Nickolas, Van Achterberg, Cornelis, Hebert, Paul, Tomanović, Željko, "Phylogeny of the Subtribe Monoctonina (Hymenoptera, Braconidae, Aphidiinae)" in Insects, 11, no. 160 (2020),
https://doi.org/10.3390/insects11030160 . .

TY  - JOUR
AU  - Kocić, Korana
AU  - Petrović, Anđeljko
AU  - Čkrkić, Jelisaveta
AU  - Mitrović, Milana
AU  - Tomanović, Željko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/864
AB  - In this study two molecular markers were used to establish taxonomic status and phylogenetic relationships of Ephedrus subgenera and species distributed in Europe. Fifteen of the nineteen currently known species have been analysed, representing three subgenera: Breviephedrus Gärdenfors, 1986, Lysephedrus Starý, 1958 and Ephedrus Haliday, 1833. The results of analysis of COI and EF1α molecular markers and morphological studies did not support this classification. Three clades separated by the highest genetic distances reported for the subfamily Aphidiinae on intrageneric level. Ephedrus brevis is separated from persicae and plagiator species groups with genetic distances of 19.6 % and 16.3 % respectively, while the distance between persicae and plagiator groups was 20.7 %. These results lead to the conclusion that the traditional subgeneric classification of Ephedrus needs revision. Species from persicae species group are raised to subgenus level as Fovephedrus Chen, 1986 and Lysephedrus syn. nov. is assigned as a junior synonym of subgenus Ephedrus. Key for identification of Ephedrus subgenera is provided. Ephedrus hyadaphidis Kocić & Tomanović sp. nov. is described and several species are confirmed as valid species for the first time. Furthermore, two species are synonymised: E. dysaphidis syn. nov. as a junior synonym of E. cerasicola and E. blattnyi syn. nov. as a junior synonym of E. plagiator.
PB  - Pensoft
T2  - ZooKeys
T1  - Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae)
EP  - 22
SP  - 1
VL  - 878
DO  - 10.3897/zookeys.878.38408
ER  - 

@article{
author = "Kocić, Korana and Petrović, Anđeljko and Čkrkić, Jelisaveta and Mitrović, Milana and Tomanović, Željko",
year = "2019",
abstract = "In this study two molecular markers were used to establish taxonomic status and phylogenetic relationships of Ephedrus subgenera and species distributed in Europe. Fifteen of the nineteen currently known species have been analysed, representing three subgenera: Breviephedrus Gärdenfors, 1986, Lysephedrus Starý, 1958 and Ephedrus Haliday, 1833. The results of analysis of COI and EF1α molecular markers and morphological studies did not support this classification. Three clades separated by the highest genetic distances reported for the subfamily Aphidiinae on intrageneric level. Ephedrus brevis is separated from persicae and plagiator species groups with genetic distances of 19.6 % and 16.3 % respectively, while the distance between persicae and plagiator groups was 20.7 %. These results lead to the conclusion that the traditional subgeneric classification of Ephedrus needs revision. Species from persicae species group are raised to subgenus level as Fovephedrus Chen, 1986 and Lysephedrus syn. nov. is assigned as a junior synonym of subgenus Ephedrus. Key for identification of Ephedrus subgenera is provided. Ephedrus hyadaphidis Kocić & Tomanović sp. nov. is described and several species are confirmed as valid species for the first time. Furthermore, two species are synonymised: E. dysaphidis syn. nov. as a junior synonym of E. cerasicola and E. blattnyi syn. nov. as a junior synonym of E. plagiator.",
publisher = "Pensoft",
journal = "ZooKeys",
title = "Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae)",
pages = "22-1",
volume = "878",
doi = "10.3897/zookeys.878.38408"
}

Kocić, K., Petrović, A., Čkrkić, J., Mitrović, M.,& Tomanović, Ž.. (2019). Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae). in ZooKeys
Pensoft., 878, 1-22.
https://doi.org/10.3897/zookeys.878.38408

Kocić K, Petrović A, Čkrkić J, Mitrović M, Tomanović Ž. Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae). in ZooKeys. 2019;878:1-22.
doi:10.3897/zookeys.878.38408 .

Kocić, Korana, Petrović, Anđeljko, Čkrkić, Jelisaveta, Mitrović, Milana, Tomanović, Željko, "Phylogenetic relationships and subgeneric classification of European Ephedrus species (Hymenoptera, Braconidae, Aphidiinae)" in ZooKeys, 878 (2019):1-22,
https://doi.org/10.3897/zookeys.878.38408 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Stary, Etr
AU  - Jakovljević, Miljana
AU  - Petrović, Anđeljko
AU  - Zikić, Vladimir
AU  - Perez Hidalgo, Nicolas
AU  - Tomanović, Željko
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/571
AB  - Species from the genus Paralipsis are obligatory endoparasitoids of root aphids in the Palaearctic. It is known that these species are broadly distributed, parasitizing various aphid hosts and showing great biological and ecological diversity. On the other hand, this group of endoparasitoids is understudied and was thought to be represented by a single species in Europe, viz., Paralipsis enervis (Nees). However, recent description of two new species indicated the possibility of cryptic speciation and recognition of additional Paralipsis species in Europe. In this research, Paralipsis specimens collected during the last 60 years from eight European countries, as well as one sample from Morocco, were subjected to molecular and morphological characterization. Newly designed genus-specific degenerative primers successfully targeted short overlapping fragments of COI of the mitochondrial DNA. Molecular analyses showed clear separation of four independent lineages, two of which are the known species P. enervis and P. tibiator, while two new species are described here, viz., P. brachycaudi Tomanovic & Stary, sp. n. and P. rugosa Tomanovic & Stary, sp. n. No clear specialization of the taxa to a strict root aphid host has been determined. The recognized mitochondrial lineages were distinct one from another, but with a substantial within-lineage divergence rate, clearly indicating the complexity of this group of parasitoids, on which further research is required in order to clarify the factors triggering their genetic differentiation. We reviewed literature data and new records of Paralipsis enervis aphid host associations and distributions. A key for the identification of all known Paralipsis species is provided and illustrated.
PB  - Pensoft Publishers
T2  - Zookeys
T1  - Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species
EP  - 69
IS  - 831
SP  - 49
DO  - 10.3897/zookeys.831.31808
ER  - 

@article{
author = "Mitrović, Milana and Stary, Etr and Jakovljević, Miljana and Petrović, Anđeljko and Zikić, Vladimir and Perez Hidalgo, Nicolas and Tomanović, Željko",
year = "2019",
abstract = "Species from the genus Paralipsis are obligatory endoparasitoids of root aphids in the Palaearctic. It is known that these species are broadly distributed, parasitizing various aphid hosts and showing great biological and ecological diversity. On the other hand, this group of endoparasitoids is understudied and was thought to be represented by a single species in Europe, viz., Paralipsis enervis (Nees). However, recent description of two new species indicated the possibility of cryptic speciation and recognition of additional Paralipsis species in Europe. In this research, Paralipsis specimens collected during the last 60 years from eight European countries, as well as one sample from Morocco, were subjected to molecular and morphological characterization. Newly designed genus-specific degenerative primers successfully targeted short overlapping fragments of COI of the mitochondrial DNA. Molecular analyses showed clear separation of four independent lineages, two of which are the known species P. enervis and P. tibiator, while two new species are described here, viz., P. brachycaudi Tomanovic & Stary, sp. n. and P. rugosa Tomanovic & Stary, sp. n. No clear specialization of the taxa to a strict root aphid host has been determined. The recognized mitochondrial lineages were distinct one from another, but with a substantial within-lineage divergence rate, clearly indicating the complexity of this group of parasitoids, on which further research is required in order to clarify the factors triggering their genetic differentiation. We reviewed literature data and new records of Paralipsis enervis aphid host associations and distributions. A key for the identification of all known Paralipsis species is provided and illustrated.",
publisher = "Pensoft Publishers",
journal = "Zookeys",
title = "Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species",
pages = "69-49",
number = "831",
doi = "10.3897/zookeys.831.31808"
}

Mitrović, M., Stary, E., Jakovljević, M., Petrović, A., Zikić, V., Perez Hidalgo, N.,& Tomanović, Ž.. (2019). Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species. in Zookeys
Pensoft Publishers.(831), 49-69.
https://doi.org/10.3897/zookeys.831.31808

Mitrović M, Stary E, Jakovljević M, Petrović A, Zikić V, Perez Hidalgo N, Tomanović Ž. Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species. in Zookeys. 2019;(831):49-69.
doi:10.3897/zookeys.831.31808 .

Mitrović, Milana, Stary, Etr, Jakovljević, Miljana, Petrović, Anđeljko, Zikić, Vladimir, Perez Hidalgo, Nicolas, Tomanović, Željko, "Integrative taxonomy of root aphid parasitoids from the genus Paralipsis (Hymenoptera, Braconidae, Aphidiinae) with description of new species" in Zookeys, no. 831 (2019):49-69,
https://doi.org/10.3897/zookeys.831.31808 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Tomanović, Željko
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/559
AB  - Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve successful amplification of the short fragments of a target gene in the event of DNA fragmentation. We tested the suitability of a noninvasive method of DNA extraction and amplification of the barcoding region of the mitochondrial gene cytochrome c oxidise subunit I from archival specimens of aphid parasitoids belonging to the genera Aphidius, Lysiphlebus and Praon (Aphidiinae, Braconidae, Hymenoptera). Using a commercial kit as a noninvasive method, we successfully extracted DNA from dry 7 to 41 year old samples of 26 different parasitoid species. However, amplification of the barcoding region failed using the standard primer pair LCO1490/HCO2198. In order to reconstruct DNA barcodes we designed internal genus-specific degenerative primers and a new amplification protocol to target the short fragments within the mitochondrial region. Novel primers were designed using as a template the reference sequences from congeners retrieved from the public database. The combination of standard primers with internal primers, in direct and nested amplification reactions, produced short overlapping subsequences, concatenated to recover long barcoding sequences. Additional analyses also confirmed that primers initially designed for Aphidius, Lysiphk.hus and Praon can be combined in a mixture, and successfully used to obtain short fragments of disintegrated DNA from archival specimens of several other braconid species from the genera Ephedrus and Monoctonus.
PB  - Pensoft Publishers
T2  - Journal of Hymenoptera Research
T1  - New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)
EP  - 210
SP  - 191
VL  - 64
DO  - 10.3897/jhr.64.25399
ER  - 

@article{
author = "Mitrović, Milana and Tomanović, Željko",
year = "2018",
abstract = "Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve successful amplification of the short fragments of a target gene in the event of DNA fragmentation. We tested the suitability of a noninvasive method of DNA extraction and amplification of the barcoding region of the mitochondrial gene cytochrome c oxidise subunit I from archival specimens of aphid parasitoids belonging to the genera Aphidius, Lysiphlebus and Praon (Aphidiinae, Braconidae, Hymenoptera). Using a commercial kit as a noninvasive method, we successfully extracted DNA from dry 7 to 41 year old samples of 26 different parasitoid species. However, amplification of the barcoding region failed using the standard primer pair LCO1490/HCO2198. In order to reconstruct DNA barcodes we designed internal genus-specific degenerative primers and a new amplification protocol to target the short fragments within the mitochondrial region. Novel primers were designed using as a template the reference sequences from congeners retrieved from the public database. The combination of standard primers with internal primers, in direct and nested amplification reactions, produced short overlapping subsequences, concatenated to recover long barcoding sequences. Additional analyses also confirmed that primers initially designed for Aphidius, Lysiphk.hus and Praon can be combined in a mixture, and successfully used to obtain short fragments of disintegrated DNA from archival specimens of several other braconid species from the genera Ephedrus and Monoctonus.",
publisher = "Pensoft Publishers",
journal = "Journal of Hymenoptera Research",
title = "New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)",
pages = "210-191",
volume = "64",
doi = "10.3897/jhr.64.25399"
}

Mitrović, M.,& Tomanović, Ž.. (2018). New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae). in Journal of Hymenoptera Research
Pensoft Publishers., 64, 191-210.
https://doi.org/10.3897/jhr.64.25399

Mitrović M, Tomanović Ž. New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae). in Journal of Hymenoptera Research. 2018;64:191-210.
doi:10.3897/jhr.64.25399 .

Mitrović, Milana, Tomanović, Željko, "New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)" in Journal of Hymenoptera Research, 64 (2018):191-210,
https://doi.org/10.3897/jhr.64.25399 . .

TY  - JOUR
AU  - Petrović, A.
AU  - Mitrović, Milana
AU  - Ghaliow, M.E.
AU  - Ivanović, Ana
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Tomanović, Željko
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/527
AB  - Species that belong to the Aphidius eadyi group have been used as biocontrol agents against Acyrthosiphon pisum worldwide. However, despite their extensive use, there are still gaps in our knowledge about their taxonomy and distribution. In this study, we employed an integrative taxonomic approach by combining genetic analyses (mtDNA COI barcoding) with standard morphological analyses and geometric morphometrics of forewing shape. We identified three species within the A. eadyi species group, viz., A. smithi, A. eadyi and A. banksae. Genetic separation of all three species was confirmed, with mean genetic distances between species ranging from 5 to 7.4%. The following morphological characters were determined as the most important for separating species of the A. eadyi group: number and shape of costulae on the anterolateral part of the petiole, shape of the central areola on the propodeum, and shape and venation of the forewings. The differences in wing shape of all three species were statistically significant, but with some overlapping. We identified A. banksae as a widely distributed pea aphid parasitoid, whose known range covers most of the western Palaearctic (from the UK to Israel). Aphidius banksae is diagnosed and redescribed.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach
EP  - 14
SP  - 1
DO  - 10.1017/S000748531800055X
ER  - 

@article{
author = "Petrović, A. and Mitrović, Milana and Ghaliow, M.E. and Ivanović, Ana and Kavallieratos, Nickolas G. and Stary, Petr and Tomanović, Željko",
year = "2018",
abstract = "Species that belong to the Aphidius eadyi group have been used as biocontrol agents against Acyrthosiphon pisum worldwide. However, despite their extensive use, there are still gaps in our knowledge about their taxonomy and distribution. In this study, we employed an integrative taxonomic approach by combining genetic analyses (mtDNA COI barcoding) with standard morphological analyses and geometric morphometrics of forewing shape. We identified three species within the A. eadyi species group, viz., A. smithi, A. eadyi and A. banksae. Genetic separation of all three species was confirmed, with mean genetic distances between species ranging from 5 to 7.4%. The following morphological characters were determined as the most important for separating species of the A. eadyi group: number and shape of costulae on the anterolateral part of the petiole, shape of the central areola on the propodeum, and shape and venation of the forewings. The differences in wing shape of all three species were statistically significant, but with some overlapping. We identified A. banksae as a widely distributed pea aphid parasitoid, whose known range covers most of the western Palaearctic (from the UK to Israel). Aphidius banksae is diagnosed and redescribed.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach",
pages = "14-1",
doi = "10.1017/S000748531800055X"
}

Petrović, A., Mitrović, M., Ghaliow, M.E., Ivanović, A., Kavallieratos, N. G., Stary, P.,& Tomanović, Ž.. (2018). Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 1-14.
https://doi.org/10.1017/S000748531800055X

Petrović A, Mitrović M, Ghaliow M, Ivanović A, Kavallieratos NG, Stary P, Tomanović Ž. Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach. in Bulletin of Entomological Research. 2018;:1-14.
doi:10.1017/S000748531800055X .

Petrović, A., Mitrović, Milana, Ghaliow, M.E., Ivanović, Ana, Kavallieratos, Nickolas G., Stary, Petr, Tomanović, Željko, "Resolving the taxonomic status of biocontrol agents belonging to the Aphidius eadyi species group (Hymenoptera: Braconidae: Aphidiinae): an integrative approach" in Bulletin of Entomological Research (2018):1-14,
https://doi.org/10.1017/S000748531800055X . .

TY  - JOUR
AU  - Tomanović, Željko
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Zikić, Vladimir
AU  - Ivanović, Ana
AU  - Rakhshani, Ehsan
AU  - Stary, Petr
AU  - Vorburger, Christoph
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/519
AB  - Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.
PB  - Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden
T2  - Arthropod Systematics & Phylogeny
T1  - Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology
EP  - 213
IS  - 2
SP  - 179
VL  - 76
ER  - 

@article{
author = "Tomanović, Željko and Mitrović, Milana and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Zikić, Vladimir and Ivanović, Ana and Rakhshani, Ehsan and Stary, Petr and Vorburger, Christoph",
year = "2018",
abstract = "Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.",
publisher = "Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden",
journal = "Arthropod Systematics & Phylogeny",
title = "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology",
pages = "213-179",
number = "2",
volume = "76"
}

Tomanović, Ž., Mitrović, M., Petrović, A., Kavallieratos, N. G., Zikić, V., Ivanović, A., Rakhshani, E., Stary, P.,& Vorburger, C.. (2018). Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny
Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden., 76(2), 179-213.

Tomanović Ž, Mitrović M, Petrović A, Kavallieratos NG, Zikić V, Ivanović A, Rakhshani E, Stary P, Vorburger C. Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny. 2018;76(2):179-213..

Tomanović, Željko, Mitrović, Milana, Petrović, Anđeljko, Kavallieratos, Nickolas G., Zikić, Vladimir, Ivanović, Ana, Rakhshani, Ehsan, Stary, Petr, Vorburger, Christoph, "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology" in Arthropod Systematics & Phylogeny, 76, no. 2 (2018):179-213.

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Ckrkić, Jelisaveta
AU  - Jamhour, Aiman
AU  - Petrović-Obradović, Olivera
AU  - Mitrović, Milana
AU  - Stary, Petr
AU  - Nedstam, Barbro
AU  - Tomanović, Željko
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/483
AB  - Aphidius ericaphidis, an aphid parasitoid originally described from North America, is recorded in Europe for the first time, based on morphological and molecular analyses. The species is recorded in Serbia, the Netherlands, Sweden and the United Kingdom. It was formerly recorded as Aphidius sp./Ericaphis latifrons/Vaccinium uliginosum from the Alps (France). The possible origin of A. ericaphidis, as well as its likely distribution, is discussed in relation to its host. As it is a specific parasitoid of Ericaphis aphids, especially the invasive aphid Ericaphis scammelli on blueberries, its potential as a biocontrol agent is also discussed.
PB  - Pensoft Publishers
T2  - Journal of Hymenoptera Research
T1  - First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?
EP  - 153
SP  - 143
VL  - 57
DO  - 10.3897/jhr.57.12517
ER  - 

@article{
author = "Petrović, Anđeljko and Ckrkić, Jelisaveta and Jamhour, Aiman and Petrović-Obradović, Olivera and Mitrović, Milana and Stary, Petr and Nedstam, Barbro and Tomanović, Željko",
year = "2017",
abstract = "Aphidius ericaphidis, an aphid parasitoid originally described from North America, is recorded in Europe for the first time, based on morphological and molecular analyses. The species is recorded in Serbia, the Netherlands, Sweden and the United Kingdom. It was formerly recorded as Aphidius sp./Ericaphis latifrons/Vaccinium uliginosum from the Alps (France). The possible origin of A. ericaphidis, as well as its likely distribution, is discussed in relation to its host. As it is a specific parasitoid of Ericaphis aphids, especially the invasive aphid Ericaphis scammelli on blueberries, its potential as a biocontrol agent is also discussed.",
publisher = "Pensoft Publishers",
journal = "Journal of Hymenoptera Research",
title = "First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?",
pages = "153-143",
volume = "57",
doi = "10.3897/jhr.57.12517"
}

Petrović, A., Ckrkić, J., Jamhour, A., Petrović-Obradović, O., Mitrović, M., Stary, P., Nedstam, B.,& Tomanović, Ž.. (2017). First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?. in Journal of Hymenoptera Research
Pensoft Publishers., 57, 143-153.
https://doi.org/10.3897/jhr.57.12517

Petrović A, Ckrkić J, Jamhour A, Petrović-Obradović O, Mitrović M, Stary P, Nedstam B, Tomanović Ž. First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?. in Journal of Hymenoptera Research. 2017;57:143-153.
doi:10.3897/jhr.57.12517 .

Petrović, Anđeljko, Ckrkić, Jelisaveta, Jamhour, Aiman, Petrović-Obradović, Olivera, Mitrović, Milana, Stary, Petr, Nedstam, Barbro, Tomanović, Željko, "First record of Aphidius ericaphidis (Hymenoptera, Braconidae) in Europe: North American hitchhiker or overlooked Holarctic citizen?" in Journal of Hymenoptera Research, 57 (2017):143-153,
https://doi.org/10.3897/jhr.57.12517 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Tomanović, Željko
AU  - Jakovljević, Miljana
AU  - Radović, D.
AU  - Havelka, J.
AU  - Stary, Petr
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/446
AB  - Populations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1 alpha gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains
EP  - 662
IS  - 5
SP  - 651
VL  - 106
DO  - 10.1017/S0007485316000377
ER  - 

@article{
author = "Mitrović, Milana and Tomanović, Željko and Jakovljević, Miljana and Radović, D. and Havelka, J. and Stary, Petr",
year = "2016",
abstract = "Populations of Liparus glabrirostris (Curculionidae: Molytinae), a weevil inhabiting higher altitudes of Central Europe, were sampled from 24 localities in the Alps and Carpathian Mountains, and the geographical structuring of genetic variation was analyzed. Comparison of the concatenated mitochondrial cytochrome oxidase subunit I and subunit II sequences revealed consistent genetic divergence between the populations of L. glabrirostris from different mountain ranges. In phylogenetic analysis using maximum parsimony and median-joining networks, concatenated mitochondrial haplotypes from the Alps and Carpathians clustered as separate lineages, with high bootstrap support. Substantial genetic distances determined between the separated groups ranged from 2.6 to 3.0%, with divergence estimated to have initiated approximately 0.85-0.98 million years ago. The nuclear elongation factor 1 alpha gene was additionally amplified and haplotype analysis showed very low evolutionary divergence (0.2%), with separate clustering as well. The observed divergence suggests that the populations have been isolated for a long time, as a consequence of environmental changes resulting in varying fragmentation of habitats in the Alps and Carpathians, interrupting genetic exchange events and altering the genetic structure of L. glabrirostris populations. On the other hand, comparison of morphological characteristics showed no differences to confirm genetically well differentiated groups of populations. A polymerase chain reaction and restriction fragment length polymorphism-based method was therefore developed to discriminate between the Alpine and Carpathian lineages.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains",
pages = "662-651",
number = "5",
volume = "106",
doi = "10.1017/S0007485316000377"
}

Mitrović, M., Tomanović, Ž., Jakovljević, M., Radović, D., Havelka, J.,& Stary, P.. (2016). Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 106(5), 651-662.
https://doi.org/10.1017/S0007485316000377

Mitrović M, Tomanović Ž, Jakovljević M, Radović D, Havelka J, Stary P. Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains. in Bulletin of Entomological Research. 2016;106(5):651-662.
doi:10.1017/S0007485316000377 .

Mitrović, Milana, Tomanović, Željko, Jakovljević, Miljana, Radović, D., Havelka, J., Stary, Petr, "Genetic differentiation of Liparus glabrirostris (Curculionidae: Molytinae) populations from the fragmented habitats of the Alps and Carpathian Mountains" in Bulletin of Entomological Research, 106, no. 5 (2016):651-662,
https://doi.org/10.1017/S0007485316000377 . .

TY  - JOUR
AU  - Jamhour, Aiman
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Stary, Petr
AU  - Tomanović, Željko
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/435
AB  - Here we tested Aphidius urticae s. str. host-associated lineages from Microlophium carnosum (Buckton), Amphorophora rubi (Kaltenbach), Macrosiphum funestum (Macchiati) and Aulacorthum vaccinii Hille Ris Lambers with the barcoding region of the mitochondrial cytochrome oxidase subunit I gene used to analyse population differences and elucidate phylogenetic relationships between the separated taxa. This molecular marker has been shown to be the most informative molecular marker in resolving species complexes in aphidiine parasitoids. Analyses of the mitochondrial sequences revealed the existence of three clearly separated mitochondrial lineages of A. urticae s. str. group associated with: i) Macrosiphum funestum and Aulacorthum vaccinii aphid hosts, ii) Microlophium carnosum and iii) Amphorophora rubi. This corresponds to the initial descriptions of A. rubi, A. silvaticus and A. urticae and their aphid host associations prior to synonymization of A. rubi and A. silvaticus with A. urticae. On the other hand, significant evolutionary distances ranging from 2.3 to 9.2% between the three mitochondrial lineages were not accompanied by clear morphological differences. Therefore, re-descriptions of A. rubi and A. silvaticus are presented, together with their morphological differentiation in a key, as well as their phylogenetic relationships and genetical differentiation.
PB  - Magnolia Press, Auckland
T2  - Zootaxa
T1  - Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)
EP  - 288
IS  - 2
SP  - 278
VL  - 4178
DO  - 10.11646/zootaxa.4178.2.6
ER  - 

@article{
author = "Jamhour, Aiman and Mitrović, Milana and Petrović, Anđeljko and Stary, Petr and Tomanović, Željko",
year = "2016",
abstract = "Here we tested Aphidius urticae s. str. host-associated lineages from Microlophium carnosum (Buckton), Amphorophora rubi (Kaltenbach), Macrosiphum funestum (Macchiati) and Aulacorthum vaccinii Hille Ris Lambers with the barcoding region of the mitochondrial cytochrome oxidase subunit I gene used to analyse population differences and elucidate phylogenetic relationships between the separated taxa. This molecular marker has been shown to be the most informative molecular marker in resolving species complexes in aphidiine parasitoids. Analyses of the mitochondrial sequences revealed the existence of three clearly separated mitochondrial lineages of A. urticae s. str. group associated with: i) Macrosiphum funestum and Aulacorthum vaccinii aphid hosts, ii) Microlophium carnosum and iii) Amphorophora rubi. This corresponds to the initial descriptions of A. rubi, A. silvaticus and A. urticae and their aphid host associations prior to synonymization of A. rubi and A. silvaticus with A. urticae. On the other hand, significant evolutionary distances ranging from 2.3 to 9.2% between the three mitochondrial lineages were not accompanied by clear morphological differences. Therefore, re-descriptions of A. rubi and A. silvaticus are presented, together with their morphological differentiation in a key, as well as their phylogenetic relationships and genetical differentiation.",
publisher = "Magnolia Press, Auckland",
journal = "Zootaxa",
title = "Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)",
pages = "288-278",
number = "2",
volume = "4178",
doi = "10.11646/zootaxa.4178.2.6"
}

Jamhour, A., Mitrović, M., Petrović, A., Stary, P.,& Tomanović, Ž.. (2016). Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae). in Zootaxa
Magnolia Press, Auckland., 4178(2), 278-288.
https://doi.org/10.11646/zootaxa.4178.2.6

Jamhour A, Mitrović M, Petrović A, Stary P, Tomanović Ž. Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae). in Zootaxa. 2016;4178(2):278-288.
doi:10.11646/zootaxa.4178.2.6 .

Jamhour, Aiman, Mitrović, Milana, Petrović, Anđeljko, Stary, Petr, Tomanović, Željko, "Re-visiting the Aphidius urticae s. str. group: re-description of Aphidius rubi Stary and A. silvaticus Stary (Hymenoptera: Braconidae: Aphidiinae)" in Zootaxa, 4178, no. 2 (2016):278-288,
https://doi.org/10.11646/zootaxa.4178.2.6 . .

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Mitrovski-Bogdanović, Ana S
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/418
AB  - Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.
PB  - BMC
T2  - BMC Evolutionary Biology
T1  - Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode
VL  - 15
DO  - 10.1186/s12862-015-0293-5
ER  - 

@article{
author = "Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Kavallieratos, Nickolas G. and Stary, Petr and Mitrovski-Bogdanović, Ana S and Tomanović, Željko and Vorburger, Christoph",
year = "2015",
abstract = "Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.",
publisher = "BMC",
journal = "BMC Evolutionary Biology",
title = "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode",
volume = "15",
doi = "10.1186/s12862-015-0293-5"
}

Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Kavallieratos, N. G., Stary, P., Mitrovski-Bogdanović, A. S., Tomanović, Ž.,& Vorburger, C.. (2015). Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology
BMC., 15.
https://doi.org/10.1186/s12862-015-0293-5

Petrović A, Mitrović M, Ivanović A, Zikić V, Kavallieratos NG, Stary P, Mitrovski-Bogdanović AS, Tomanović Ž, Vorburger C. Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology. 2015;15.
doi:10.1186/s12862-015-0293-5 .

Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Kavallieratos, Nickolas G., Stary, Petr, Mitrovski-Bogdanović, Ana S, Tomanović, Željko, Vorburger, Christoph, "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode" in BMC Evolutionary Biology, 15 (2015),
https://doi.org/10.1186/s12862-015-0293-5 . .

TY  - JOUR
AU  - Tomanović, Željko
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Rakhshani, Ehsan
AU  - Rakhshanipour, M.
AU  - Popović, A.
AU  - Shukshuk, A. H.
AU  - Ivanović, Ana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/336
AB  - We have identified the following three taxa related to the Aphidius colemani species group, which are important biological control agents: Aphidius colemani, Aphidius transcaspicus and Aphidius platensis. Using partial sequences of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene and geometric morphometric analysis of the forewing shape, we have explored the genetic structure and morphological variability of the A. colemani group from different aphid host/plant associations covering a wide distribution area. The topology of the maximum parsimony and maximum likelihood trees were identical with 98-100% bootstrap support, clustering A. colemani, A. platensis and A. transcaspicus into separate species. The distances among the taxa ranged from 2.2 to 4.7%, which is a common rate for the between-species divergence within the subfamily Aphidiinae. Differences in the shape of the forewing investigated within the biotypes of A. colemani group are congruent with their genetic diversification. Both A. platensis and A. colemani share a common host range pattern, and it would be interesting to estimate and compare the role of these two species in future biological control strategies against aphids of economic importance. Our results indicate that 'genetic screening' is a reliable approach for identification within the A. colemani group. The high variation in the wing shape among species, including a significant divergence in the wing shape among specimens that emerged from different hosts, makes the forewing shape and wing venation less reliable for species determination. Aphidius platensis is diagnostified and redescribed, and the key for the A. colemani group is presented.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae)
EP  - 565
IS  - 5
SP  - 552
VL  - 104
DO  - 10.1017/S0007485314000327
ER  - 

@article{
author = "Tomanović, Željko and Petrović, Anđeljko and Mitrović, Milana and Kavallieratos, Nickolas G. and Stary, Petr and Rakhshani, Ehsan and Rakhshanipour, M. and Popović, A. and Shukshuk, A. H. and Ivanović, Ana",
year = "2014",
abstract = "We have identified the following three taxa related to the Aphidius colemani species group, which are important biological control agents: Aphidius colemani, Aphidius transcaspicus and Aphidius platensis. Using partial sequences of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene and geometric morphometric analysis of the forewing shape, we have explored the genetic structure and morphological variability of the A. colemani group from different aphid host/plant associations covering a wide distribution area. The topology of the maximum parsimony and maximum likelihood trees were identical with 98-100% bootstrap support, clustering A. colemani, A. platensis and A. transcaspicus into separate species. The distances among the taxa ranged from 2.2 to 4.7%, which is a common rate for the between-species divergence within the subfamily Aphidiinae. Differences in the shape of the forewing investigated within the biotypes of A. colemani group are congruent with their genetic diversification. Both A. platensis and A. colemani share a common host range pattern, and it would be interesting to estimate and compare the role of these two species in future biological control strategies against aphids of economic importance. Our results indicate that 'genetic screening' is a reliable approach for identification within the A. colemani group. The high variation in the wing shape among species, including a significant divergence in the wing shape among specimens that emerged from different hosts, makes the forewing shape and wing venation less reliable for species determination. Aphidius platensis is diagnostified and redescribed, and the key for the A. colemani group is presented.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae)",
pages = "565-552",
number = "5",
volume = "104",
doi = "10.1017/S0007485314000327"
}

Tomanović, Ž., Petrović, A., Mitrović, M., Kavallieratos, N. G., Stary, P., Rakhshani, E., Rakhshanipour, M., Popović, A., Shukshuk, A. H.,& Ivanović, A.. (2014). Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 104(5), 552-565.
https://doi.org/10.1017/S0007485314000327

Tomanović Ž, Petrović A, Mitrović M, Kavallieratos NG, Stary P, Rakhshani E, Rakhshanipour M, Popović A, Shukshuk AH, Ivanović A. Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae). in Bulletin of Entomological Research. 2014;104(5):552-565.
doi:10.1017/S0007485314000327 .

Tomanović, Željko, Petrović, Anđeljko, Mitrović, Milana, Kavallieratos, Nickolas G., Stary, Petr, Rakhshani, Ehsan, Rakhshanipour, M., Popović, A., Shukshuk, A. H., Ivanović, Ana, "Molecular and morphological variability within the Aphidius colemani group with redescription of Aphidius platensis Brethes (Hymenoptera: Braconidae: Aphidiinae)" in Bulletin of Entomological Research, 104, no. 5 (2014):552-565,
https://doi.org/10.1017/S0007485314000327 . .

TY  - JOUR
AU  - Mitrovski-Bogdanović, Ana S
AU  - Tomanović, Željko
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Stary, Petr
AU  - Vorburger, Christoph
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/344
AB  - In this paper we apply an integrative approach combining morphometric and molecular analyses to explore parasitoids of the Praon dorsale-yomenae s.str. complex. These parasitoids occur in natural and agricultural ecosystems throughout the Palaearctic and parasitize aphid hosts belonging to the tribe Macrosiphini. The P. dorsale-yomenae species complex represents a morphologically cryptic group, consisting of several hidden taxa with specific host adaptations and distributions. For the morphometric analyses we used a large dataset comprising 230 female specimens that emerged from 30 different species of aphid hosts throughout the Palaearctic. The molecular analysis included a reduced dataset of 44 specimens that emerged from 19 aphid hosts. The mitochondrial cytochrome oxidase subunit I (COI) and the nuclear second expansion segment of the 285 rRNA gene (28SD2) were used to estimate a genetic diversity within this complex. Although all Praon species clustered closely together in the 28SD2 tree, confirming their close relatedness, the molecular characterization based on COI identified five clearly separate taxa with sequence divergences in the range of 4.7-8.9%. These taxa also exhibited significant differences in forewing shape as revealed by geometric morphometric analyses. Classical morphometric analyses revealed the length of m-cu vein as a new taxonomic character, but suggested that one commonly used trait, the color pattern of the Rs + M and m-cu veins, cannot be used for species distinction as it was highly variable in one of the taxa. Based on the combined morphometric and genetic data, we confirm the species status of Praon dorsale, P. yomenae, P. longicorne and P. volucre, and describe and illustrate a new parasitoid species of the "dorsale-yomenae" group, i.e. Praon staticobii n.sp. associated with Staticobium limonii on Limonium angustifolium. (C) 2014 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Zoologischer Anzeiger
T1  - The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species
EP  - 282
IS  - 4
SP  - 270
VL  - 253
DO  - 10.1016/j.jcz.2014.02.001
ER  - 

@article{
author = "Mitrovski-Bogdanović, Ana S and Tomanović, Željko and Mitrović, Milana and Petrović, Anđeljko and Ivanović, Ana and Zikić, Vladimir and Stary, Petr and Vorburger, Christoph",
year = "2014",
abstract = "In this paper we apply an integrative approach combining morphometric and molecular analyses to explore parasitoids of the Praon dorsale-yomenae s.str. complex. These parasitoids occur in natural and agricultural ecosystems throughout the Palaearctic and parasitize aphid hosts belonging to the tribe Macrosiphini. The P. dorsale-yomenae species complex represents a morphologically cryptic group, consisting of several hidden taxa with specific host adaptations and distributions. For the morphometric analyses we used a large dataset comprising 230 female specimens that emerged from 30 different species of aphid hosts throughout the Palaearctic. The molecular analysis included a reduced dataset of 44 specimens that emerged from 19 aphid hosts. The mitochondrial cytochrome oxidase subunit I (COI) and the nuclear second expansion segment of the 285 rRNA gene (28SD2) were used to estimate a genetic diversity within this complex. Although all Praon species clustered closely together in the 28SD2 tree, confirming their close relatedness, the molecular characterization based on COI identified five clearly separate taxa with sequence divergences in the range of 4.7-8.9%. These taxa also exhibited significant differences in forewing shape as revealed by geometric morphometric analyses. Classical morphometric analyses revealed the length of m-cu vein as a new taxonomic character, but suggested that one commonly used trait, the color pattern of the Rs + M and m-cu veins, cannot be used for species distinction as it was highly variable in one of the taxa. Based on the combined morphometric and genetic data, we confirm the species status of Praon dorsale, P. yomenae, P. longicorne and P. volucre, and describe and illustrate a new parasitoid species of the "dorsale-yomenae" group, i.e. Praon staticobii n.sp. associated with Staticobium limonii on Limonium angustifolium. (C) 2014 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Zoologischer Anzeiger",
title = "The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species",
pages = "282-270",
number = "4",
volume = "253",
doi = "10.1016/j.jcz.2014.02.001"
}

Mitrovski-Bogdanović, A. S., Tomanović, Ž., Mitrović, M., Petrović, A., Ivanović, A., Zikić, V., Stary, P.,& Vorburger, C.. (2014). The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species. in Zoologischer Anzeiger
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 253(4), 270-282.
https://doi.org/10.1016/j.jcz.2014.02.001

Mitrovski-Bogdanović AS, Tomanović Ž, Mitrović M, Petrović A, Ivanović A, Zikić V, Stary P, Vorburger C. The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species. in Zoologischer Anzeiger. 2014;253(4):270-282.
doi:10.1016/j.jcz.2014.02.001 .

Mitrovski-Bogdanović, Ana S, Tomanović, Željko, Mitrović, Milana, Petrović, Anđeljko, Ivanović, Ana, Zikić, Vladimir, Stary, Petr, Vorburger, Christoph, "The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species" in Zoologischer Anzeiger, 253, no. 4 (2014):270-282,
https://doi.org/10.1016/j.jcz.2014.02.001 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Petrović-Obradović, Olivera
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/297
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/584
AB  - Lysiphlebus testaceipes (Cress.) is an aphidiine parasitoid originally introduced to Europe as a biological control agent of citrus aphids in the Mediterranean. It has rapidly become widespread in coastal areas continuing gradually to expand inland. L. testaceipes exploited a large number of aphids in Europe, including new hosts and significantly changed the relative abundance of the native parasitoids. This behavior may reflect a broad oligophagy of the introduced parasitoid or it may require the evolution of host specialization that results in genetically differentiated subpopulations on different hosts. To address this issue we used the mitochondrial cytochrome oxidase subunit I and seven microsatellite loci to analyze the structure of genetic variation for L. testaceipes samples collected from 12 different aphid hosts across seven European countries, as well as some samples from Benin, Costa Rica, USA, Algeria and Libya for comparison. Only five COI haplotypes with moderate divergence were identified overall. There was no evidence for the association of haplotypes with different aphid hosts in the European samples, but there was geographic structuring in this variation. Haplotype diversity was highest in France, where L. testaceipes was introduced, but only a single haplotype was detected in areas of south-eastern Europe that were invaded subsequently. The analysis of microsatellite variation confirmed the lack of host-associated genetic structure, as well as differentiation between populations from south-western and southeastern Europe. The parasitoid L. testaceipes in Europe is thus an opportunistic oligophagous species with a population structure shaped by the processes of introduction and expansion rather than by host exploitation. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent
EP  - 158
IS  - 3
SP  - 150
VL  - 66
DO  - 10.1016/j.biocontrol.2013.05.007
ER  - 

@article{
author = "Mitrović, Milana and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Stary, Petr and Petrović-Obradović, Olivera and Tomanović, Željko and Vorburger, Christoph",
year = "2013",
abstract = "Lysiphlebus testaceipes (Cress.) is an aphidiine parasitoid originally introduced to Europe as a biological control agent of citrus aphids in the Mediterranean. It has rapidly become widespread in coastal areas continuing gradually to expand inland. L. testaceipes exploited a large number of aphids in Europe, including new hosts and significantly changed the relative abundance of the native parasitoids. This behavior may reflect a broad oligophagy of the introduced parasitoid or it may require the evolution of host specialization that results in genetically differentiated subpopulations on different hosts. To address this issue we used the mitochondrial cytochrome oxidase subunit I and seven microsatellite loci to analyze the structure of genetic variation for L. testaceipes samples collected from 12 different aphid hosts across seven European countries, as well as some samples from Benin, Costa Rica, USA, Algeria and Libya for comparison. Only five COI haplotypes with moderate divergence were identified overall. There was no evidence for the association of haplotypes with different aphid hosts in the European samples, but there was geographic structuring in this variation. Haplotype diversity was highest in France, where L. testaceipes was introduced, but only a single haplotype was detected in areas of south-eastern Europe that were invaded subsequently. The analysis of microsatellite variation confirmed the lack of host-associated genetic structure, as well as differentiation between populations from south-western and southeastern Europe. The parasitoid L. testaceipes in Europe is thus an opportunistic oligophagous species with a population structure shaped by the processes of introduction and expansion rather than by host exploitation. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent",
pages = "158-150",
number = "3",
volume = "66",
doi = "10.1016/j.biocontrol.2013.05.007"
}

Mitrović, M., Petrović, A., Kavallieratos, N. G., Stary, P., Petrović-Obradović, O., Tomanović, Ž.,& Vorburger, C.. (2013). Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 66(3), 150-158.
https://doi.org/10.1016/j.biocontrol.2013.05.007

Mitrović M, Petrović A, Kavallieratos NG, Stary P, Petrović-Obradović O, Tomanović Ž, Vorburger C. Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent. in Biological Control. 2013;66(3):150-158.
doi:10.1016/j.biocontrol.2013.05.007 .

Mitrović, Milana, Petrović, Anđeljko, Kavallieratos, Nickolas G., Stary, Petr, Petrović-Obradović, Olivera, Tomanović, Željko, Vorburger, Christoph, "Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent" in Biological Control, 66, no. 3 (2013):150-158,
https://doi.org/10.1016/j.biocontrol.2013.05.007 . .

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Stary, Petr
AU  - Petrović-Obradović, Olivera
AU  - Zikić, Vladimir
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/296
AB  - We report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers
EP  - 457
IS  - 4
SP  - 451
VL  - 103
DO  - 10.1017/S0007485313000035
ER  - 

@article{
author = "Petrović, Anđeljko and Mitrović, Milana and Stary, Petr and Petrović-Obradović, Olivera and Zikić, Vladimir and Tomanović, Željko and Vorburger, Christoph",
year = "2013",
abstract = "We report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers",
pages = "457-451",
number = "4",
volume = "103",
doi = "10.1017/S0007485313000035"
}

Petrović, A., Mitrović, M., Stary, P., Petrović-Obradović, O., Zikić, V., Tomanović, Ž.,& Vorburger, C.. (2013). Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 103(4), 451-457.
https://doi.org/10.1017/S0007485313000035

Petrović A, Mitrović M, Stary P, Petrović-Obradović O, Zikić V, Tomanović Ž, Vorburger C. Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers. in Bulletin of Entomological Research. 2013;103(4):451-457.
doi:10.1017/S0007485313000035 .

Petrović, Anđeljko, Mitrović, Milana, Stary, Petr, Petrović-Obradović, Olivera, Zikić, Vladimir, Tomanović, Željko, Vorburger, Christoph, "Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers" in Bulletin of Entomological Research, 103, no. 4 (2013):451-457,
https://doi.org/10.1017/S0007485313000035 . .

TY  - JOUR
AU  - Mitrovski-Bogdanović, Ana S
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Stary, Petr
AU  - Vorburger, Christoph
AU  - Tomanović, Željko
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/289
AB  - The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.
PB  - Oxford Univ Press Inc, Cary
T2  - Annals of the Entomological Society of America
T1  - Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics
EP  - 180
IS  - 2
SP  - 170
VL  - 106
DO  - 10.1603/AN12100
ER  - 

@article{
author = "Mitrovski-Bogdanović, Ana S and Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Stary, Petr and Vorburger, Christoph and Tomanović, Željko",
year = "2013",
abstract = "The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Annals of the Entomological Society of America",
title = "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics",
pages = "180-170",
number = "2",
volume = "106",
doi = "10.1603/AN12100"
}

Mitrovski-Bogdanović, A. S., Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Stary, P., Vorburger, C.,& Tomanović, Ž.. (2013). Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America
Oxford Univ Press Inc, Cary., 106(2), 170-180.
https://doi.org/10.1603/AN12100

Mitrovski-Bogdanović AS, Petrović A, Mitrović M, Ivanović A, Zikić V, Stary P, Vorburger C, Tomanović Ž. Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America. 2013;106(2):170-180.
doi:10.1603/AN12100 .

Mitrovski-Bogdanović, Ana S, Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Stary, Petr, Vorburger, Christoph, Tomanović, Željko, "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics" in Annals of the Entomological Society of America, 106, no. 2 (2013):170-180,
https://doi.org/10.1603/AN12100 . .

TY  - BOOK
AU  - Tomanović, Željko
AU  - Petanović, Radmila
AU  - Petrović-Obradović, Olivera
AU  - Tosevski, Ivo
AU  - Petrović, Anđeljko
AU  - Tomanović, Snežana
AU  - Jovic, Jelena
AU  - Stanisavljević, Ljubiša
AU  - Lazarević, Jelica
AU  - Ćurčić, Srećko
AU  - Živič, Ivana
AU  - Mihajlović, Ljubodrag
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1079
PB  - Beograd : Univerzitet, Biološki fakultet
T2  - Beograd : Univerzitet, Biološki fakultet
T1  - Primenjena entomologija
ER  - 

@book{
author = "Tomanović, Željko and Petanović, Radmila and Petrović-Obradović, Olivera and Tosevski, Ivo and Petrović, Anđeljko and Tomanović, Snežana and Jovic, Jelena and Stanisavljević, Ljubiša and Lazarević, Jelica and Ćurčić, Srećko and Živič, Ivana and Mihajlović, Ljubodrag",
year = "2012",
publisher = "Beograd : Univerzitet, Biološki fakultet",
journal = "Beograd : Univerzitet, Biološki fakultet",
title = "Primenjena entomologija"
}

Tomanović, Ž., Petanović, R., Petrović-Obradović, O., Tosevski, I., Petrović, A., Tomanović, S., Jovic, J., Stanisavljević, L., Lazarević, J., Ćurčić, S., Živič, I.,& Mihajlović, L.. (2012). Primenjena entomologija. in Beograd : Univerzitet, Biološki fakultet
Beograd : Univerzitet, Biološki fakultet..

Tomanović Ž, Petanović R, Petrović-Obradović O, Tosevski I, Petrović A, Tomanović S, Jovic J, Stanisavljević L, Lazarević J, Ćurčić S, Živič I, Mihajlović L. Primenjena entomologija. in Beograd : Univerzitet, Biološki fakultet. 2012;..

Tomanović, Željko, Petanović, Radmila, Petrović-Obradović, Olivera, Tosevski, Ivo, Petrović, Anđeljko, Tomanović, Snežana, Jovic, Jelena, Stanisavljević, Ljubiša, Lazarević, Jelica, Ćurčić, Srećko, Živič, Ivana, Mihajlović, Ljubodrag, "Primenjena entomologija" in Beograd : Univerzitet, Biološki fakultet (2012).

TY  - JOUR
AU  - Kos, Katarina
AU  - Petrović, Anđeljko
AU  - Stary, Petr
AU  - Kavallieratos, Nickolas G.
AU  - Ivanović, Ana
AU  - Toševski, Ivo
AU  - Jakse, J
AU  - Trdan, Stanislav
AU  - Tomanović, Željko
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/169
AB  - In this study, the relationships among and the taxonomic status of three closely related parasitic wasps that are widely used as biological control agents of cereal aphids, Aphidius uzbekistanicus Luzhetzki, Aphidius rhopalosiphi De Stefani Perez, and Aphidius avenaphis (Fitch), were examined. Genetic divergence at an average of 6% was recorded between A. uzbekistanicus and A. rhopalosiphi by using the mitochondrial (mt) gene cytochrome oxidase I (COI) barcoding region. Identical mtCOI gene sequences were observed in A. uzbekistanicus specimens that originated from Eurasia and in the North American species A. avenaphis. The haplotype fluctuation in A. rhopalosiphi specimens that originated from the west Palaearctic was an average of 1.5% (maximum, 2.4%). In contrast, specimens of A. uzbekistanicus from central and western parts of Eurasia were largely homogenous, with only a single mutation recorded in a specimen from eastern Europe (Serbia). The morphological and genetic diversity found in A. rhopalosiphi may suggest the existence of cryptic species, especially for lineages that have a large degree of mtCOI diversity and sympatric occurrence. The geometric morphometric analysis of stigma shape presented in this study demonstrated that members of A. uzbekistanicus have a shorter forewing r vein and a more elongated stigma, relative to those of A. avenaphis. Our research validates the use of stigma shape and flagellomere 1 color for morphological discrimination between wasp species.
PB  - Entomological Soc Amer, Lanham
T2  - Annals of the Entomological Society of America
T1  - On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology
EP  - 1232
IS  - 6
SP  - 1221
VL  - 104
DO  - 10.1603/AN11055
ER  - 

@article{
author = "Kos, Katarina and Petrović, Anđeljko and Stary, Petr and Kavallieratos, Nickolas G. and Ivanović, Ana and Toševski, Ivo and Jakse, J and Trdan, Stanislav and Tomanović, Željko",
year = "2011",
abstract = "In this study, the relationships among and the taxonomic status of three closely related parasitic wasps that are widely used as biological control agents of cereal aphids, Aphidius uzbekistanicus Luzhetzki, Aphidius rhopalosiphi De Stefani Perez, and Aphidius avenaphis (Fitch), were examined. Genetic divergence at an average of 6% was recorded between A. uzbekistanicus and A. rhopalosiphi by using the mitochondrial (mt) gene cytochrome oxidase I (COI) barcoding region. Identical mtCOI gene sequences were observed in A. uzbekistanicus specimens that originated from Eurasia and in the North American species A. avenaphis. The haplotype fluctuation in A. rhopalosiphi specimens that originated from the west Palaearctic was an average of 1.5% (maximum, 2.4%). In contrast, specimens of A. uzbekistanicus from central and western parts of Eurasia were largely homogenous, with only a single mutation recorded in a specimen from eastern Europe (Serbia). The morphological and genetic diversity found in A. rhopalosiphi may suggest the existence of cryptic species, especially for lineages that have a large degree of mtCOI diversity and sympatric occurrence. The geometric morphometric analysis of stigma shape presented in this study demonstrated that members of A. uzbekistanicus have a shorter forewing r vein and a more elongated stigma, relative to those of A. avenaphis. Our research validates the use of stigma shape and flagellomere 1 color for morphological discrimination between wasp species.",
publisher = "Entomological Soc Amer, Lanham",
journal = "Annals of the Entomological Society of America",
title = "On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology",
pages = "1232-1221",
number = "6",
volume = "104",
doi = "10.1603/AN11055"
}

Kos, K., Petrović, A., Stary, P., Kavallieratos, N. G., Ivanović, A., Toševski, I., Jakse, J., Trdan, S.,& Tomanović, Ž.. (2011). On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology. in Annals of the Entomological Society of America
Entomological Soc Amer, Lanham., 104(6), 1221-1232.
https://doi.org/10.1603/AN11055

Kos K, Petrović A, Stary P, Kavallieratos NG, Ivanović A, Toševski I, Jakse J, Trdan S, Tomanović Ž. On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology. in Annals of the Entomological Society of America. 2011;104(6):1221-1232.
doi:10.1603/AN11055 .

Kos, Katarina, Petrović, Anđeljko, Stary, Petr, Kavallieratos, Nickolas G., Ivanović, Ana, Toševski, Ivo, Jakse, J, Trdan, Stanislav, Tomanović, Željko, "On the Identity of Cereal Aphid Parasitoid Wasps Aphidius uzbekistanicus, Aphidius rhopalosiphi, and Aphidius avenaphis (Hymenoptera: Braconidae: Aphidiinae) by Examination of COI Mitochondrial Gene, Geometric Morphometrics, and Morphology" in Annals of the Entomological Society of America, 104, no. 6 (2011):1221-1232,
https://doi.org/10.1603/AN11055 . .

TY  - JOUR
AU  - Tomanović, Željko
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Toševski, Ivo
AU  - Mitrovski-Bogdanović, Ana S
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/97
AB  - Areopraon chaitophori n. sp. associated with Chaitophorus leucomelas Koch on Populus nigra is described and illustrated. The main diagnostic characters of Areopraon species are discussed, and a key for identification of European species is provided. The distal dorsal part of the second metasomal tergite of Areopraon species has a very prominent horizontal longitudinal protuberance, which represents a new diagnostic character for Areopraon.
PB  - Soc Entomologique France, Paris
T2  - Annales de la Societe Entomologique de France
T1  - Areopraon chaitophori n. sp (Hymenoptera: Braconidae: Aphidiinae) associated with Chaitophorus leucomelas Koch on poplars, with a key for European Areopraon Mackauer species
EP  - 192
IS  - 2
SP  - 187
VL  - 45
DO  - 10.1080/00379271.2009.10697601
ER  - 

@article{
author = "Tomanović, Željko and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Stary, Petr and Toševski, Ivo and Mitrovski-Bogdanović, Ana S",
year = "2009",
abstract = "Areopraon chaitophori n. sp. associated with Chaitophorus leucomelas Koch on Populus nigra is described and illustrated. The main diagnostic characters of Areopraon species are discussed, and a key for identification of European species is provided. The distal dorsal part of the second metasomal tergite of Areopraon species has a very prominent horizontal longitudinal protuberance, which represents a new diagnostic character for Areopraon.",
publisher = "Soc Entomologique France, Paris",
journal = "Annales de la Societe Entomologique de France",
title = "Areopraon chaitophori n. sp (Hymenoptera: Braconidae: Aphidiinae) associated with Chaitophorus leucomelas Koch on poplars, with a key for European Areopraon Mackauer species",
pages = "192-187",
number = "2",
volume = "45",
doi = "10.1080/00379271.2009.10697601"
}

Tomanović, Ž., Petrović, A., Kavallieratos, N. G., Stary, P., Toševski, I.,& Mitrovski-Bogdanović, A. S.. (2009). Areopraon chaitophori n. sp (Hymenoptera: Braconidae: Aphidiinae) associated with Chaitophorus leucomelas Koch on poplars, with a key for European Areopraon Mackauer species. in Annales de la Societe Entomologique de France
Soc Entomologique France, Paris., 45(2), 187-192.
https://doi.org/10.1080/00379271.2009.10697601

Tomanović Ž, Petrović A, Kavallieratos NG, Stary P, Toševski I, Mitrovski-Bogdanović AS. Areopraon chaitophori n. sp (Hymenoptera: Braconidae: Aphidiinae) associated with Chaitophorus leucomelas Koch on poplars, with a key for European Areopraon Mackauer species. in Annales de la Societe Entomologique de France. 2009;45(2):187-192.
doi:10.1080/00379271.2009.10697601 .

Tomanović, Željko, Petrović, Anđeljko, Kavallieratos, Nickolas G., Stary, Petr, Toševski, Ivo, Mitrovski-Bogdanović, Ana S, "Areopraon chaitophori n. sp (Hymenoptera: Braconidae: Aphidiinae) associated with Chaitophorus leucomelas Koch on poplars, with a key for European Areopraon Mackauer species" in Annales de la Societe Entomologique de France, 45, no. 2 (2009):187-192,
https://doi.org/10.1080/00379271.2009.10697601 . .