TY  - JOUR
AU  - Tomanović, Željko
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Zikić, Vladimir
AU  - Ivanović, Ana
AU  - Rakhshani, Ehsan
AU  - Stary, Petr
AU  - Vorburger, Christoph
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/519
AB  - Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.
PB  - Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden
T2  - Arthropod Systematics & Phylogeny
T1  - Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology
EP  - 213
IS  - 2
SP  - 179
VL  - 76
ER  - 

@article{
author = "Tomanović, Željko and Mitrović, Milana and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Zikić, Vladimir and Ivanović, Ana and Rakhshani, Ehsan and Stary, Petr and Vorburger, Christoph",
year = "2018",
abstract = "Members of the genus Lysiphlebus Foerster are medium-sized endoparasitoid aphidiine wasps with over 30 species distributed worldwide. They attack numerous aphid hosts, and some species are important biological control agents. All European Lysiphlebus species are revised based on sequence information from the mitochondrial COI barcoding gene and the nuclear 28SD2 gene, morphological traits, and on information about their host specificity. The taxonomic status of 13 European Lysiphlebus species is confirmed, while 11 are redescribed and illustrated. The following four Lysiphlebus species are synonymized: L. melandriicola Stary, 1961 [= L. fabarum (Marshall, 1896)], L. hirtus Stark, 1985 (= L. confusus Tremblay & Eady, 1978), L. hispanus Stary, 1973 (= L. desertorum Stary, 1965), and L. safavii Stary, 1985 (= L. hirticornis Mackauer, 1960). Two new Lysiphlebus species are described: L. volkli sp.n. and L. brachycaudi sp.n. Our results, based on both COI and 28SD2, confirm the existence of three monophyletic species groups within the genus: (1) the 'fabarum' group, which includes L. fabarum, L. cardui (Marshall, 1896), L. confusus, L. hirticornis, L. brachycaudi sp.n., and L. volkli sp.n.; (2) the 'testaceipes' group, which includes L. testaceipes (Cresson, 1880), L. orientalis Stary & Rakhshani, 2010, L. fritzmuelleri Mackauer, 1960, L. balcanicus Stary, 1998, L. desertorum, [and probably L. dissolutus (Nees, 1811)]; and (3) the 'alpinus' group, consisting solely of L. alpinus Stary, 1971. Geometric morphometrics of fore wing shape proved to be a powerful tool to delineate sibling species within the fabarum' group. We were able to confirm for the first time sexual lines of L. cardui and L. confusus. Furthermore, an additional potentially asexual Lysiphlebus taxon in Europe is revealed based on the discovery of an asexual line of L. volkli sp.n. in Iran. An illustrated key for identification of the European Lysiphlebus species is provided.",
publisher = "Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden",
journal = "Arthropod Systematics & Phylogeny",
title = "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology",
pages = "213-179",
number = "2",
volume = "76"
}

Tomanović, Ž., Mitrović, M., Petrović, A., Kavallieratos, N. G., Zikić, V., Ivanović, A., Rakhshani, E., Stary, P.,& Vorburger, C.. (2018). Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny
Senckenberg Naturhistorische Sammlungen Dresden, Museum Tierkunde, Dresden., 76(2), 179-213.

Tomanović Ž, Mitrović M, Petrović A, Kavallieratos NG, Zikić V, Ivanović A, Rakhshani E, Stary P, Vorburger C. Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology. in Arthropod Systematics & Phylogeny. 2018;76(2):179-213..

Tomanović, Željko, Mitrović, Milana, Petrović, Anđeljko, Kavallieratos, Nickolas G., Zikić, Vladimir, Ivanović, Ana, Rakhshani, Ehsan, Stary, Petr, Vorburger, Christoph, "Revision of the European Lysiphlebus species (Hymenoptera: Braconidae: Aphidiinae) on the basis of COI and 28SD2 molecular markers and morphology" in Arthropod Systematics & Phylogeny, 76, no. 2 (2018):179-213.

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Mitrovski-Bogdanović, Ana S
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/418
AB  - Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.
PB  - BMC
T2  - BMC Evolutionary Biology
T1  - Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode
VL  - 15
DO  - 10.1186/s12862-015-0293-5
ER  - 

@article{
author = "Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Kavallieratos, Nickolas G. and Stary, Petr and Mitrovski-Bogdanović, Ana S and Tomanović, Željko and Vorburger, Christoph",
year = "2015",
abstract = "Background: Morphological divergence often increases with phylogenetic distance, thus making morphology taxonomically informative. However, transitions to asexual reproduction may complicate this relationship because asexual lineages capture and freeze parts of the phenotypic variation of the sexual populations from which they derive. Parasitoid wasps belonging to the genus Lysiphlebus Foerster (Hymenoptera: Braconidae: Aphidiinae) are composed of over 20 species that exploit over a hundred species of aphid hosts, including many important agricultural pests. Within Lysiphlebus, two genetically and morphologically well- defined species groups are recognised: the "fabarum" and the "testaceipes" groups. Yet within each group, sexual as well as asexual lineages occur, and in L. fabarum different morphs of unknown origin and status have been recognised. In this study, we selected a broad sample of specimens from the genus Lysiphlebus to explore the relationship between genetic divergence, reproductive mode and morphological variation in wing size and shape (quantified by geometric morphometrics). Results: The analyses of mitochondrial and nuclear gene sequences revealed a clear separation between the "testaceipes" and "fabarum" groups of Lysiphlebus, as well as three well-defined phylogenetic lineages within the "fabarum" species group and two lineages within the "testaceipes" group. Divergence in wing shape was concordant with the deep split between the "testaceipes" and "fabarum" species groups, but within groups no clear association between genetic divergence and wing shape variation was observed. On the other hand, we found significant and consistent differences in the shape of the wing between sexual and asexual lineages, even when they were closely related. Conclusions: Mapping wing shape data onto an independently derived molecular phylogeny of Lysiphlebus revealed an association between genetic and morphological divergence only for the deepest phylogenetic split. In more recently diverged taxa, much of the variation in wing shape was explained by differences between sexual and asexual lineages, suggesting a mechanistic link between wing shape and reproductive mode in these parasitoid wasps.",
publisher = "BMC",
journal = "BMC Evolutionary Biology",
title = "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode",
volume = "15",
doi = "10.1186/s12862-015-0293-5"
}

Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Kavallieratos, N. G., Stary, P., Mitrovski-Bogdanović, A. S., Tomanović, Ž.,& Vorburger, C.. (2015). Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology
BMC., 15.
https://doi.org/10.1186/s12862-015-0293-5

Petrović A, Mitrović M, Ivanović A, Zikić V, Kavallieratos NG, Stary P, Mitrovski-Bogdanović AS, Tomanović Ž, Vorburger C. Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode. in BMC Evolutionary Biology. 2015;15.
doi:10.1186/s12862-015-0293-5 .

Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Kavallieratos, Nickolas G., Stary, Petr, Mitrovski-Bogdanović, Ana S, Tomanović, Željko, Vorburger, Christoph, "Genetic and morphological variation in sexual and asexual parasitoids of the genus Lysiphlebus - an apparent link between wing shape and reproductive mode" in BMC Evolutionary Biology, 15 (2015),
https://doi.org/10.1186/s12862-015-0293-5 . .

TY  - JOUR
AU  - Mitrovski-Bogdanović, Ana S
AU  - Tomanović, Željko
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Stary, Petr
AU  - Vorburger, Christoph
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/344
AB  - In this paper we apply an integrative approach combining morphometric and molecular analyses to explore parasitoids of the Praon dorsale-yomenae s.str. complex. These parasitoids occur in natural and agricultural ecosystems throughout the Palaearctic and parasitize aphid hosts belonging to the tribe Macrosiphini. The P. dorsale-yomenae species complex represents a morphologically cryptic group, consisting of several hidden taxa with specific host adaptations and distributions. For the morphometric analyses we used a large dataset comprising 230 female specimens that emerged from 30 different species of aphid hosts throughout the Palaearctic. The molecular analysis included a reduced dataset of 44 specimens that emerged from 19 aphid hosts. The mitochondrial cytochrome oxidase subunit I (COI) and the nuclear second expansion segment of the 285 rRNA gene (28SD2) were used to estimate a genetic diversity within this complex. Although all Praon species clustered closely together in the 28SD2 tree, confirming their close relatedness, the molecular characterization based on COI identified five clearly separate taxa with sequence divergences in the range of 4.7-8.9%. These taxa also exhibited significant differences in forewing shape as revealed by geometric morphometric analyses. Classical morphometric analyses revealed the length of m-cu vein as a new taxonomic character, but suggested that one commonly used trait, the color pattern of the Rs + M and m-cu veins, cannot be used for species distinction as it was highly variable in one of the taxa. Based on the combined morphometric and genetic data, we confirm the species status of Praon dorsale, P. yomenae, P. longicorne and P. volucre, and describe and illustrate a new parasitoid species of the "dorsale-yomenae" group, i.e. Praon staticobii n.sp. associated with Staticobium limonii on Limonium angustifolium. (C) 2014 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Zoologischer Anzeiger
T1  - The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species
EP  - 282
IS  - 4
SP  - 270
VL  - 253
DO  - 10.1016/j.jcz.2014.02.001
ER  - 

@article{
author = "Mitrovski-Bogdanović, Ana S and Tomanović, Željko and Mitrović, Milana and Petrović, Anđeljko and Ivanović, Ana and Zikić, Vladimir and Stary, Petr and Vorburger, Christoph",
year = "2014",
abstract = "In this paper we apply an integrative approach combining morphometric and molecular analyses to explore parasitoids of the Praon dorsale-yomenae s.str. complex. These parasitoids occur in natural and agricultural ecosystems throughout the Palaearctic and parasitize aphid hosts belonging to the tribe Macrosiphini. The P. dorsale-yomenae species complex represents a morphologically cryptic group, consisting of several hidden taxa with specific host adaptations and distributions. For the morphometric analyses we used a large dataset comprising 230 female specimens that emerged from 30 different species of aphid hosts throughout the Palaearctic. The molecular analysis included a reduced dataset of 44 specimens that emerged from 19 aphid hosts. The mitochondrial cytochrome oxidase subunit I (COI) and the nuclear second expansion segment of the 285 rRNA gene (28SD2) were used to estimate a genetic diversity within this complex. Although all Praon species clustered closely together in the 28SD2 tree, confirming their close relatedness, the molecular characterization based on COI identified five clearly separate taxa with sequence divergences in the range of 4.7-8.9%. These taxa also exhibited significant differences in forewing shape as revealed by geometric morphometric analyses. Classical morphometric analyses revealed the length of m-cu vein as a new taxonomic character, but suggested that one commonly used trait, the color pattern of the Rs + M and m-cu veins, cannot be used for species distinction as it was highly variable in one of the taxa. Based on the combined morphometric and genetic data, we confirm the species status of Praon dorsale, P. yomenae, P. longicorne and P. volucre, and describe and illustrate a new parasitoid species of the "dorsale-yomenae" group, i.e. Praon staticobii n.sp. associated with Staticobium limonii on Limonium angustifolium. (C) 2014 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Zoologischer Anzeiger",
title = "The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species",
pages = "282-270",
number = "4",
volume = "253",
doi = "10.1016/j.jcz.2014.02.001"
}

Mitrovski-Bogdanović, A. S., Tomanović, Ž., Mitrović, M., Petrović, A., Ivanović, A., Zikić, V., Stary, P.,& Vorburger, C.. (2014). The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species. in Zoologischer Anzeiger
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 253(4), 270-282.
https://doi.org/10.1016/j.jcz.2014.02.001

Mitrovski-Bogdanović AS, Tomanović Ž, Mitrović M, Petrović A, Ivanović A, Zikić V, Stary P, Vorburger C. The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species. in Zoologischer Anzeiger. 2014;253(4):270-282.
doi:10.1016/j.jcz.2014.02.001 .

Mitrovski-Bogdanović, Ana S, Tomanović, Željko, Mitrović, Milana, Petrović, Anđeljko, Ivanović, Ana, Zikić, Vladimir, Stary, Petr, Vorburger, Christoph, "The Praon dorsale-yomenae s.str. complex (Hymenoptera, Braconidae, Aphidiinae): Species discrimination using geometric morphometrics and molecular markers with description of a new species" in Zoologischer Anzeiger, 253, no. 4 (2014):270-282,
https://doi.org/10.1016/j.jcz.2014.02.001 . .

TY  - JOUR
AU  - Mitrović, Milana
AU  - Petrović, Anđeljko
AU  - Kavallieratos, Nickolas G.
AU  - Stary, Petr
AU  - Petrović-Obradović, Olivera
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/297
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/584
AB  - Lysiphlebus testaceipes (Cress.) is an aphidiine parasitoid originally introduced to Europe as a biological control agent of citrus aphids in the Mediterranean. It has rapidly become widespread in coastal areas continuing gradually to expand inland. L. testaceipes exploited a large number of aphids in Europe, including new hosts and significantly changed the relative abundance of the native parasitoids. This behavior may reflect a broad oligophagy of the introduced parasitoid or it may require the evolution of host specialization that results in genetically differentiated subpopulations on different hosts. To address this issue we used the mitochondrial cytochrome oxidase subunit I and seven microsatellite loci to analyze the structure of genetic variation for L. testaceipes samples collected from 12 different aphid hosts across seven European countries, as well as some samples from Benin, Costa Rica, USA, Algeria and Libya for comparison. Only five COI haplotypes with moderate divergence were identified overall. There was no evidence for the association of haplotypes with different aphid hosts in the European samples, but there was geographic structuring in this variation. Haplotype diversity was highest in France, where L. testaceipes was introduced, but only a single haplotype was detected in areas of south-eastern Europe that were invaded subsequently. The analysis of microsatellite variation confirmed the lack of host-associated genetic structure, as well as differentiation between populations from south-western and southeastern Europe. The parasitoid L. testaceipes in Europe is thus an opportunistic oligophagous species with a population structure shaped by the processes of introduction and expansion rather than by host exploitation. (C) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent
EP  - 158
IS  - 3
SP  - 150
VL  - 66
DO  - 10.1016/j.biocontrol.2013.05.007
ER  - 

@article{
author = "Mitrović, Milana and Petrović, Anđeljko and Kavallieratos, Nickolas G. and Stary, Petr and Petrović-Obradović, Olivera and Tomanović, Željko and Vorburger, Christoph",
year = "2013",
abstract = "Lysiphlebus testaceipes (Cress.) is an aphidiine parasitoid originally introduced to Europe as a biological control agent of citrus aphids in the Mediterranean. It has rapidly become widespread in coastal areas continuing gradually to expand inland. L. testaceipes exploited a large number of aphids in Europe, including new hosts and significantly changed the relative abundance of the native parasitoids. This behavior may reflect a broad oligophagy of the introduced parasitoid or it may require the evolution of host specialization that results in genetically differentiated subpopulations on different hosts. To address this issue we used the mitochondrial cytochrome oxidase subunit I and seven microsatellite loci to analyze the structure of genetic variation for L. testaceipes samples collected from 12 different aphid hosts across seven European countries, as well as some samples from Benin, Costa Rica, USA, Algeria and Libya for comparison. Only five COI haplotypes with moderate divergence were identified overall. There was no evidence for the association of haplotypes with different aphid hosts in the European samples, but there was geographic structuring in this variation. Haplotype diversity was highest in France, where L. testaceipes was introduced, but only a single haplotype was detected in areas of south-eastern Europe that were invaded subsequently. The analysis of microsatellite variation confirmed the lack of host-associated genetic structure, as well as differentiation between populations from south-western and southeastern Europe. The parasitoid L. testaceipes in Europe is thus an opportunistic oligophagous species with a population structure shaped by the processes of introduction and expansion rather than by host exploitation. (C) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent",
pages = "158-150",
number = "3",
volume = "66",
doi = "10.1016/j.biocontrol.2013.05.007"
}

Mitrović, M., Petrović, A., Kavallieratos, N. G., Stary, P., Petrović-Obradović, O., Tomanović, Ž.,& Vorburger, C.. (2013). Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 66(3), 150-158.
https://doi.org/10.1016/j.biocontrol.2013.05.007

Mitrović M, Petrović A, Kavallieratos NG, Stary P, Petrović-Obradović O, Tomanović Ž, Vorburger C. Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent. in Biological Control. 2013;66(3):150-158.
doi:10.1016/j.biocontrol.2013.05.007 .

Mitrović, Milana, Petrović, Anđeljko, Kavallieratos, Nickolas G., Stary, Petr, Petrović-Obradović, Olivera, Tomanović, Željko, Vorburger, Christoph, "Geographic structure with no evidence for host-associated lineages in European populations of Lysiphlebus testaceipes, an introduced biological control agent" in Biological Control, 66, no. 3 (2013):150-158,
https://doi.org/10.1016/j.biocontrol.2013.05.007 . .

TY  - JOUR
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Stary, Petr
AU  - Petrović-Obradović, Olivera
AU  - Zikić, Vladimir
AU  - Tomanović, Željko
AU  - Vorburger, Christoph
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/296
AB  - We report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers
EP  - 457
IS  - 4
SP  - 451
VL  - 103
DO  - 10.1017/S0007485313000035
ER  - 

@article{
author = "Petrović, Anđeljko and Mitrović, Milana and Stary, Petr and Petrović-Obradović, Olivera and Zikić, Vladimir and Tomanović, Željko and Vorburger, Christoph",
year = "2013",
abstract = "We report the occurrence of Lysiphlebus orientalis in Serbia, an aphid parasitoid from the Far East that is new to Europe and has the potential to become invasive. Our finding based on morphological characters is confirmed by analyses of mitochondrial cytochrome oxidase subunit I sequences. An increase in number and an expansion of the host range were observed during field studies over the past two years, and it is determined that the current host range encompasses nine aphid hosts on 12 different host plants, forming 13 tri-trophic associations. A host range determined for European populations of L. orientalis appears wider compared with that in its Far Eastern native habitats where Aphis glycines Mats. is the sole known host. Moreover, it overlaps considerably with the host ranges of European parasitoids that play an important role in the natural control of pest aphids.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers",
pages = "457-451",
number = "4",
volume = "103",
doi = "10.1017/S0007485313000035"
}

Petrović, A., Mitrović, M., Stary, P., Petrović-Obradović, O., Zikić, V., Tomanović, Ž.,& Vorburger, C.. (2013). Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers. in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 103(4), 451-457.
https://doi.org/10.1017/S0007485313000035

Petrović A, Mitrović M, Stary P, Petrović-Obradović O, Zikić V, Tomanović Ž, Vorburger C. Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers. in Bulletin of Entomological Research. 2013;103(4):451-457.
doi:10.1017/S0007485313000035 .

Petrović, Anđeljko, Mitrović, Milana, Stary, Petr, Petrović-Obradović, Olivera, Zikić, Vladimir, Tomanović, Željko, Vorburger, Christoph, "Lysiphlebus orientalis (Hymenoptera, Braconidae), a new invasive aphid parasitoid in Europe - evidence from molecular markers" in Bulletin of Entomological Research, 103, no. 4 (2013):451-457,
https://doi.org/10.1017/S0007485313000035 . .

TY  - JOUR
AU  - Mitrovski-Bogdanović, Ana S
AU  - Petrović, Anđeljko
AU  - Mitrović, Milana
AU  - Ivanović, Ana
AU  - Zikić, Vladimir
AU  - Stary, Petr
AU  - Vorburger, Christoph
AU  - Tomanović, Željko
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/289
AB  - The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.
PB  - Oxford Univ Press Inc, Cary
T2  - Annals of the Entomological Society of America
T1  - Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics
EP  - 180
IS  - 2
SP  - 170
VL  - 106
DO  - 10.1603/AN12100
ER  - 

@article{
author = "Mitrovski-Bogdanović, Ana S and Petrović, Anđeljko and Mitrović, Milana and Ivanović, Ana and Zikić, Vladimir and Stary, Petr and Vorburger, Christoph and Tomanović, Željko",
year = "2013",
abstract = "The genus Praon represents a large group of aphid endoparasitoids and is exemplary for the problems encountered in their taxonomy because of a great variability of morphological characters. To investigate the intraspecific variability and to ascertain cryptic speciation within the Praon abjectum Haliday group, biotypes in association with the aphid hosts Aphis sambuci L., Longicaudus trirhodus Walker, and Rhopalosiphum spp. were examined. We combined molecular and geometric morphometric analyses, that is, partial sequences of the mitochondrial cytochrome oxidase subunit I and nuclear 28SD2 genes and the shape of the forewing. Low variation of 28SD2 sequences confirmed the close relatedness of species from the genus Praon. Analysis of the cytochrome oxidase subunit I sequences however identified three separate taxa within the P. abjectum group with substantial genetic divergence. The biotype of P. abjectum associated with L. trirhodus differed from those associated with Rhopalosiphum sp. and A. sambuci by 5.4-6.5% and 7.7% sequence divergence, respectively, while the genetic distance between the latter two biotypes ranged from 9.5 to 10%. The main changes in the forewing shape that discriminate these three biotypes as revealed by geometric morphometrics are related to the stigma shape and the position of the radial nerve. Based on the differences determined in mitochondrial sequences and in the shape of the wing, we describe two new cryptic species within the P. abjectum group as follows: P. sambuci sp. n. in association with A. sambuci/S. nigra and P. longicaudus sp. n. in association with L. trirhodus/T. aquilegifolium.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Annals of the Entomological Society of America",
title = "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics",
pages = "180-170",
number = "2",
volume = "106",
doi = "10.1603/AN12100"
}

Mitrovski-Bogdanović, A. S., Petrović, A., Mitrović, M., Ivanović, A., Zikić, V., Stary, P., Vorburger, C.,& Tomanović, Ž.. (2013). Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America
Oxford Univ Press Inc, Cary., 106(2), 170-180.
https://doi.org/10.1603/AN12100

Mitrovski-Bogdanović AS, Petrović A, Mitrović M, Ivanović A, Zikić V, Stary P, Vorburger C, Tomanović Ž. Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics. in Annals of the Entomological Society of America. 2013;106(2):170-180.
doi:10.1603/AN12100 .

Mitrovski-Bogdanović, Ana S, Petrović, Anđeljko, Mitrović, Milana, Ivanović, Ana, Zikić, Vladimir, Stary, Petr, Vorburger, Christoph, Tomanović, Željko, "Identification of Two Cryptic Species Within the Praon abjectum Group (Hymenoptera: Braconidae: Aphidiinae) Using Molecular Markers and Geometric Morphometrics" in Annals of the Entomological Society of America, 106, no. 2 (2013):170-180,
https://doi.org/10.1603/AN12100 . .