TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1138
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - MDPI
T2  - Microorganisms
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
IS  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8, 2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms
MDPI., 11(8).
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms. 2023;11(8).
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms, 11, no. 8 (2023),
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Medić, O.
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/849
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
SP  - 105238
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Medić, O. and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "105238",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier., 182, 105238.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Medić, O., Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

Marković, S., Milić Komić, S., Jelušić, A., Iličić, R., Bagi, F., Stanković, S.,& Popović Milovanović, T.. (2022). First report of Pectobacterium versatile causing blackleg of potato in Serbia. in Plant Disease
American Phytopathological Society., 106(1).
https://doi.org/10.1094/PDIS-06-21-1128-PDN

Marković S, Milić Komić S, Jelušić A, Iličić R, Bagi F, Stanković S, Popović Milovanović T. First report of Pectobacterium versatile causing blackleg of potato in Serbia. in Plant Disease. 2022;106(1).
doi:10.1094/PDIS-06-21-1128-PDN .

Marković, Sanja, Milić Komić, Sonja, Jelušić, Aleksandra, Iličić, Renata, Bagi, Ferenc, Stanković, Slaviša, Popović Milovanović, Tatjana, "First report of Pectobacterium versatile causing blackleg of potato in Serbia" in Plant Disease, 106, no. 1 (2022),
https://doi.org/10.1094/PDIS-06-21-1128-PDN . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/684
AB  - Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected field (58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present in all samples, with Proteobacteria being the most prevalent, especially in tubers without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were more represented in tubers without symptoms. However, the genus Dickeya was at the limit of detection or not detected at all. Acinetobacter was the most dominant in tubers with soft rot, while Pseudomonas and Enterobacter were the most abundant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with starch-degradable features were almost exclusively present in soft rotted tubers and their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confirmed biocontrol potential. This study indicates that the soft rot maceration process arises due to complex interactions between plant pathogens and other endophytic bacteria.
PB  - Springer Verlag
T2  - Potato Research
T1  - Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres
DO  - 10.1007/s11540-022-09601-9
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Berić, Tanja and Dimkić, Ivica and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2022",
abstract = "Plant pathogenic bacteria from the genera Pectobacterium and Dickeya in association with potato cause blackleg and tuber soft rot disease. A metabarcoding was used to analyze the microbial communities of soft-rotted tubers and their geocaulospheres. In September 2018, samples collected from the blackleg-infected field (58 ha) in the Bačka region (Serbia) reached 45% disease incidence. Next-generation sequencing Illumina MiSeq platform was used to obtain 16S rRNA sequences from six tested tubers (with and without soft rot symptom) and the surrounding geocaulosphere. Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria were present in all samples, with Proteobacteria being the most prevalent, especially in tubers without soft rot symptoms. In all tested samples, species of the Pectobacterium (P. aroidearum, P. atrosepticum, P. carotovorum, and P. polaris) were detected but were more represented in tubers without symptoms. However, the genus Dickeya was at the limit of detection or not detected at all. Acinetobacter was the most dominant in tubers with soft rot, while Pseudomonas and Enterobacter were the most abundant in tubers without symptoms. The genera Bacteroides and Dysgonomonas with starch-degradable features were almost exclusively present in soft rotted tubers and their corresponding geocaulospheres. The most represented genera in the geocaulosphere sample associated with no-symptom tubers were Gaiella, Sphingomonas, Sphingobium, Gemmatimonas, and Geminicoccus, which include species with confirmed biocontrol potential. This study indicates that the soft rot maceration process arises due to complex interactions between plant pathogens and other endophytic bacteria.",
publisher = "Springer Verlag",
journal = "Potato Research",
title = "Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres",
doi = "10.1007/s11540-022-09601-9"
}

Marković, S., Popović Milovanović, T., Berić, T., Dimkić, I., Jelušić, A., Iličić, R.,& Stanković, S.. (2022). Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres. in Potato Research
Springer Verlag..
https://doi.org/10.1007/s11540-022-09601-9

Marković S, Popović Milovanović T, Berić T, Dimkić I, Jelušić A, Iličić R, Stanković S. Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres. in Potato Research. 2022;.
doi:10.1007/s11540-022-09601-9 .

Marković, Sanja, Popović Milovanović, Tatjana, Berić, Tanja, Dimkić, Ivica, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Metabarcoding Approach for Evaluation of Bacterial Diversity in Soft Rotting Potato Tubers and Corresponding Geocaulospheres" in Potato Research (2022),
https://doi.org/10.1007/s11540-022-09601-9 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Barać, Goran
AU  - Bagi, Ferenc
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/739
AB  - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Šančevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes—gapA, gltA, rpoD and gyrB—showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305T (=58T = LMG28609T) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +− − − + (Pseudomonas Group Ia) and GATTa + − + −, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
PB  - Wiley
T2  - Annals of Applied Biology
T1  - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
EP  - 150
IS  - 1
SP  - 140
VL  - 180
DO  - 10.1111/aab.12717
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Marković, Sanja and Barać, Goran and Bagi, Ferenc and Popović Milovanović, Tatjana",
year = "2022",
abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Šančevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes—gapA, gltA, rpoD and gyrB—showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305T (=58T = LMG28609T) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +− − − + (Pseudomonas Group Ia) and GATTa + − + −, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.",
publisher = "Wiley",
journal = "Annals of Applied Biology",
title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification",
pages = "150-140",
number = "1",
volume = "180",
doi = "10.1111/aab.12717"
}

Iličić, R., Jelušić, A., Marković, S., Barać, G., Bagi, F.,& Popović Milovanović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology
Wiley., 180(1), 140-150.
https://doi.org/10.1111/aab.12717

Iličić R, Jelušić A, Marković S, Barać G, Bagi F, Popović Milovanović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150.
doi:10.1111/aab.12717 .

Iličić, Renata, Jelušić, Aleksandra, Marković, Sanja, Barać, Goran, Bagi, Ferenc, Popović Milovanović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150,
https://doi.org/10.1111/aab.12717 . .

TY  - CONF
AU  - Iličić, Renata
AU  - Barać, Goran
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/799
AB  - BACKGROUND Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality. OBJECTIVES This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) in locality Maglić (Bačka) in 2021. METHODS Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database. RESULTS PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three (two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited strains of Ca. Phytoplasma solani.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022
T1  - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE
ER  - 

@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2022",
abstract = "BACKGROUND Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality. OBJECTIVES This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) in locality Maglić (Bačka) in 2021. METHODS Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database. RESULTS PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three (two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE"
}

Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović Milovanović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..

Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović Milovanović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022. 2022;..

Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović Milovanović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022 (2022).

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/824
AB  - BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids
(Cheers F1 and Hippo F1) grown in Futog in 2021.
METHODS
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed
with concatenated sequences.
RESULTS
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were
separated from P. carotovorum isolates, clustered into four groups.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30.06-02.07.2022. Serbia 915-915
T1  - Pathogenic bacterial population causing soft rot on cabbage: case study in Futog (Serbia)
ER  - 

@conference{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2022",
abstract = "BACKGROUND
Futog (Vojvodina, Serbia) is known for the five-century long tradition of cabbage cultivation, which is grown
in two forms during the season, summer and autumn. Pectobacteriaceae causing soft rot disease are among
the most destructive pathogens, influencing the yield and quality of this crop. These pectolytic bacteria cause
maceration of attacked tissues, often followed by specific odor caused by the colonization of saprophytic
bacteria that inhabit damaged tissues.
OBJECTIVES
Within this work, we aimed to identify soft rot-causing bacteria observed on two summer cabbage hybrids
(Cheers F1 and Hippo F1) grown in Futog in 2021.
METHODS
Crystal Violet Pectate (CVP) medium was used for bacterial isolation from six collected cabbage samples
showing soft rot. The pathogenicity of isolates was checked on cabbage heads by making holes at the top and
filling them with bacterial suspensions. Multilocus sequence typing (MLST) of cabbage isolates was performed
by sequencing of proA and dnaX genes. Additionally, the Neighbour-joining phylogenetic analysis was performed
with concatenated sequences.
RESULTS
Isolations resulted in the prevalence of pit-forming colonies on CVP. Water-soaked lesions followed by a
specific odor appeared on cabbage heads 24 h after inoculation with all tested isolates, and complete head
destruction occurred after seven days. MLST performed with genes proA and dnaX, identified isolates as
Pectobacterium carotovorum on hybrids Cheers F1 and Hippo F1 and Pectobacterium versatile only present
on Hippo F1, indicating combined infection on this hybrid. On the phylogenetic tree, P. versatile isolates were
separated from P. carotovorum isolates, clustered into four groups.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30.06-02.07.2022. Serbia 915-915",
title = "Pathogenic bacterial population causing soft rot on cabbage: case study in Futog (Serbia)"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2022). Pathogenic bacterial population causing soft rot on cabbage: case study in Futog (Serbia). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30.06-02.07.2022. Serbia 915-915
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..

Jelušić A, Mitrović P, Marković S, Iličić R, Stanković S, Popović Milovanović T. Pathogenic bacterial population causing soft rot on cabbage: case study in Futog (Serbia). in FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30.06-02.07.2022. Serbia 915-915. 2022;..

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Pathogenic bacterial population causing soft rot on cabbage: case study in Futog (Serbia)" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology 30.06-02.07.2022. Serbia 915-915 (2022).

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Aćimović, Radivoje
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/809
AB  - The vegetable soybean “edamame” (Glicine max L.) is a nutritious legume having pods and seeds that can be harvested and consumed while they are still fresh and premature. Edamame is rich in micronutrients and vitamins and is therefore used as food and for medicinal purposes. In Serbia, edamame production started for the first time in 2019 on a 10 ha field in Bečej locality (Bačka, Vojvodina). Later, in 2021, its production was expanded to 30 ha, but in summer was followed with symptoms of bacterial leaf spot. The symptoms appeared on leaves in the form of water-soaked spots surrounded by a chlorotic halo that enlarged and coalesced into necrotic lesions. Disease incidence was from 15-20%. Isolation of the causal pathogen was performed by sowing of suspension of the macerated margins of spots/lesions from ten collected symptomatic leaves on nutrient agar supplemented with 5% sucrose. Ten representative, purified isolates were whitish, circular, smooth, shiny, levan-positive, strictly aerobic, gram-negative; positive for green-fluorescent pigment and tobacco hypersensitive response, and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Pathogenicity of the isolates was confirmed on soybean cotyledons by under pressure atomizing a bacterial suspension (107-8 CFU mL 1 ). Sequencing of genes gapA, gyrB, and rpoD showed 100% homology of the obtained isolates with Pseudomonas savastanoi pv. glycinea pathotype strain LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, MOC601, R4a (gapA and gyrB), and M301765 (gapA and rpoD) originated from soybean, all from the Plant Associated and Environmental Microbes Database (PAMDB).
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124
T1  - Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a nutritious legume having pods and seeds that can be harvested and consumed while they are still fresh and premature. Edamame is rich in micronutrients and vitamins and is therefore used as food and for medicinal purposes. In Serbia, edamame production started for the first time in 2019 on a 10 ha field in Bečej locality (Bačka, Vojvodina). Later, in 2021, its production was expanded to 30 ha, but in summer was followed with symptoms of bacterial leaf spot. The symptoms appeared on leaves in the form of water-soaked spots surrounded by a chlorotic halo that enlarged and coalesced into necrotic lesions. Disease incidence was from 15-20%. Isolation of the causal pathogen was performed by sowing of suspension of the macerated margins of spots/lesions from ten collected symptomatic leaves on nutrient agar supplemented with 5% sucrose. Ten representative, purified isolates were whitish, circular, smooth, shiny, levan-positive, strictly aerobic, gram-negative; positive for green-fluorescent pigment and tobacco hypersensitive response, and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Pathogenicity of the isolates was confirmed on soybean cotyledons by under pressure atomizing a bacterial suspension (107-8 CFU mL 1 ). Sequencing of genes gapA, gyrB, and rpoD showed 100% homology of the obtained isolates with Pseudomonas savastanoi pv. glycinea pathotype strain LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, MOC601, R4a (gapA and gyrB), and M301765 (gapA and rpoD) originated from soybean, all from the Plant Associated and Environmental Microbes Database (PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124",
title = "Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia"
}

Popović Milovanović, T., Jelušić, A., Aćimović, R., Marković, S.,& Iličić, R.. (2022). Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia. in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini..

Popović Milovanović T, Jelušić A, Aćimović R, Marković S, Iličić R. Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia. in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124. 2022;..

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Aćimović, Radivoje, Marković, Sanja, Iličić, Renata, "Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia" in 14th International Conference on Plant Pathogenic Bacteria, Assisi (Italy) 3-8 July, 2022: 124-124 (2022).

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Kosovac, Andrea
AU  - Poštić, Dobrivoj
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/742
AB  - Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Bačka region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in some Potato Fields in Serbia
EP  - 1090
IS  - 4
SP  - 1080
VL  - 105
DO  - 10.1094/PDIS-05-20-1076-RE
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Kosovac, Andrea and Poštić, Dobrivoj and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Blackleg outbreaks were noticed on three fields (about 100 ha total) in 2 consecutive years (2018, 2019) in one of the main potato growing areas in Serbia (Bačka region, Vojvodina). The percentage of infected plants reached 40 to 70%, with 10.5 to 44.7% yield reductions. From the three fields, out of 90 samples Pectobacterium carotovorum subsp. brasiliensis was most frequently identified and diagnosed as causal agent of potato blackleg in Serbia for the first time (29 isolates). Dickeya dianthicola was a less frequently causative bacterium, which was also noticed for the first time (nine isolates). A total of 38 isolates were characterized based on their phenotypic and genetic features, including a pathogenicity test on potato. The repetitive element PCR (rep-PCR) using BOX, REP, and ERIC primer pairs differentiated five genetic profiles among 38 tested isolates. Multilocus sequence analysis (MLSA) of four housekeeping genes, acnA, gapA, icdA, and mdh, revealed the presence of three so far unknown P. c. subsp. brasiliensis multilocus genotypes and confirmed clustering into two main genetic clades as determined in other studies. MLSA also revealed the presence of a new genotype of D. dianthicola in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in some Potato Fields in Serbia",
pages = "1090-1080",
number = "4",
volume = "105",
doi = "10.1094/PDIS-05-20-1076-RE"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R., Kosovac, A., Poštić, D.,& Popović Milovanović, T.. (2021). Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in some Potato Fields in Serbia. in Plant Disease
American Phytopathological Society., 105(4), 1080-1090.
https://doi.org/10.1094/PDIS-05-20-1076-RE

Marković S, Stanković S, Jelušić A, Iličić R, Kosovac A, Poštić D, Popović Milovanović T. Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in some Potato Fields in Serbia. in Plant Disease. 2021;105(4):1080-1090.
doi:10.1094/PDIS-05-20-1076-RE .

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Kosovac, Andrea, Poštić, Dobrivoj, Popović Milovanović, Tatjana, "Occurrence and Identification of Pectobacterium carotovorum subsp. brasiliensis and Dickeya dianthicola Causing Blackleg in some Potato Fields in Serbia" in Plant Disease, 105, no. 4 (2021):1080-1090,
https://doi.org/10.1094/PDIS-05-20-1076-RE . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Iličić, Renata
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/821
AB  - Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa obolelih listova trešnje i šljive. U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive (lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C (gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi). PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s. pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, domaćina ili lokalitet sa kog su izolovani.
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35
T1  - Molekularna karakterizacija Pseudomonas syringae pv. morsprunorum poreklom sa trešnje i šljive u Srbiji
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Marković, Sanja and Blagojević, Milan and Bagi, Ferenc and Iličić, Renata",
year = "2021",
abstract = "Bakterija Pseudomonas syringae pv. morsprunorum, prouzrokovač bakteriozne pegavosti listova i plodova, ekonomski je značajan patogen koštičavih vrsta voćaka. Pri povoljim vremenskim uslovima za razvoj (kišovito i prohladno vreme) bolest se brzo širi zahvatajući masovno listove cele krune stabla. Pored pegavosti, P. s. pv. morsprunorum prouzrokuje simptome uvelosti, sušenja i rak rane drvenastih delova biljnih domaćina. Cilj ovog rada je da se genetički okarakterišu sojevi P. syringae pv. morsprunorum poreklom sa obolelih listova trešnje i šljive. U radu su korišćeni sojevi poreklom sa trešnje (lokalitet Topola, 2016. godina) i šljive (lokalitet Krušedol selo, 2020. godina). Genetička karakterizacija je vršena metodom analize multilokusnih sekvenci (MLSA), dobijenih korišćenjem 4 konzervisana gena: gapA, gltA, gyrB i rpoD. DNK sojeva je izolovana CTAB metodom, a amplifikacija je vršena metodom lančane reakcije polimeraze (PCR) prema sledećem programu: početna denaturacija na 94 °C u trajanju od 3 minuta, zatim 30 ciklusa denaturacije na 94 °C 2 minuta, hibridizacije na 54 °C (gapA), 56 °C (gltA), 62 °C (gyrB) ili 63 °C (rpoD) 1 minut i elongacije na 72 °C 1 minut, i finalna elongacija na 72 °C u trajanju od 10 minuta. PCR produkti su sekvencirani i dobijene sekvence su korišćene za konstruisanje filogenetskih stabala (MegaX program) na osnovu pojedinačnih i konkatamernih sekvenci. Za filogenetsku analizu su pored sojeva poreklom sa trešnje i šljive iz Srbije korišćeni i referentni sojevi vrsta prouzrokovača bakteriozne pegavosti koštičavog voća deponovani u NCBI bazi (P. s. pv. morsprunorum rase 1 i 2, P. s. pv. syringae, P. s. pv. avii, P. s. pv. persicae i P. cerasi). PCR amplifikacija je rezultirala produktima veličine 634 bp (gapA), 556 bp (gltA), 610 bp (gyrB) i 521 bp (rpoD) kod svih analiziranih sojeva. Sekvence su sređene i poravnate na veličine od 610 nt (gapA), 526 nt (gltA), 569 nt (gyrB) i 483 nt (rpoD). Na osnovu pojedinačnih gena sojevi sa trešnje i šljive iz Srbije su grupisani u jedan klaster na filogenetskom stablu, što ukazuje na njihovu genetičku homogenost. Zbirno filogenetsko stablo konstruisano na osnovu konkatamernih sekvenci sva četiri gena (2188 nt) grupisalo je sojeve sa trešnje i šljive zajedno sa P. s. pv. morsprunorum rasom 1 poreklom sa šljive iz Poljske i P. amygdali pv. morsprunorum poreklom sa trešnje iz Velike Britanije i Srbije izolovanim 2012. godine. Referentni sojevi ostalih Pseudomonas vrsta korišćenih za filogenetsku analizu jasno su odvojeni u posebne klastere. Na osnovu dobijenih rezultata može se zaključiti da su sojevi P. s. pv. morsprunorum rasa 1 poreklom iz Srbije genetički homogeni bez obzira na godinu, domaćina ili lokalitet sa kog su izolovani.",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35",
title = "Molekularna karakterizacija Pseudomonas syringae pv. morsprunorum poreklom sa trešnje i šljive u Srbiji"
}

Jelušić, A., Popović Milovanović, T., Marković, S., Blagojević, M., Bagi, F.,& Iličić, R.. (2021). Molekularna karakterizacija Pseudomonas syringae pv. morsprunorum poreklom sa trešnje i šljive u Srbiji. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35
Društvo za zaštitu bilja..

Jelušić A, Popović Milovanović T, Marković S, Blagojević M, Bagi F, Iličić R. Molekularna karakterizacija Pseudomonas syringae pv. morsprunorum poreklom sa trešnje i šljive u Srbiji. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35. 2021;..

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Marković, Sanja, Blagojević, Milan, Bagi, Ferenc, Iličić, Renata, "Molekularna karakterizacija Pseudomonas syringae pv. morsprunorum poreklom sa trešnje i šljive u Srbiji" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 34-35 (2021).

TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/798
AB  - Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi distribucija populacija u 2020. i 2021. godini. Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze (PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano (MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.
PB  - Društvo za zaštitu bilja Srbije
C3  - XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021
T1  - NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI
ER  - 

@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Bolest crna noga krompira se poslednjih godina redovno javlja na području Vojvodine, jednom od najvećih regiona gajenja ove biljne vrste. Prema literaturnim izvorima, tokom 1990-ih bakterije Pectobacterium carotovorum subsp. carotovorum i Pectobacterium atrosepticum se navode kao glavni prouzrokovači ove bolesti, a novija istraživanja ukazuju na sve češću prisutnost prouzrokovača Pectobacterium brasiliense, a u pojedinačnim slučajevima i Dickeya dianthicola. Obzirom na utvrđeni diverzitet, cilj ovog rada je bio da se izvrši identifikacija prouzrokovača crne noge krompira na području Bačke (Vojvodina) i utvrdi distribucija populacija u 2020. i 2021. godini. Tokom jula 2020. i 2021. godine vršen je monitoring useva krompira i sakupljeno je 40 uzoraka sa simptomima crne noge. Tokom 2020. u lokalitetu Maglić uzorci su prikupljeni sa sorti VR808 (28 ha) i Brooke (18 ha), a tokom 2021. iz lokaliteta Maglić sa sorti VR808 (10,2 ha), Kiebitz (18,6 ha), Brooke (9 ha) i nepoznate sorte (1,5 ha) i lokaliteta Sombor sa sorti VR808 (52 ha) i Pirol (41 ha). Izolacija je vršena na kristal violet pektat podlogu (CVP), a čiste kulture održavane su na hranljivom agaru (NA). Patogenost je testirana na kriškama krompira i metodom uboda u stablo mladih biljaka krompira. Metodom lančane reakcije polimeraze (PCR) sa parom prajmera dnaXf/dnaXr vršena je amplifikacija DNK izolata i dobijeni produkti su sekvencirani. BLASTn program je korišćen za poređenje sekvenci izolata poreklom sa krompira iz Srbije sa deponovanim sojevima u NCBI bazi. Filogenetsko stablo je konstruisano (MegaX softver) za utvrđivanje srodnosti izolata sa referentnim sojevima istih vrsta. Nakon izvršene izolacije, na CVP podlozi su dominirale beličasto-krem kolonije koje su formirale udubljenja u podlozi. Za dalji rad odabrano je 92 izolata. Svi izolati su nakon 24 sata izazvali maceraciju kriški krompira uz prisustvo neprijatnog mirisa. Na mladim biljkama krompira početni simptomi su se javili dva dana nakon inokulacije u vidu vodenih lezija na mestu uboda, koje su se kasnije širile duž stabljike u vidu nekroze. Posle petog dana od inokulacije biljke su potpuno propadale. Prema rezultatima analiziranih sekvenci dnaX gena, izolati sa krompira iz 2020. su identifikovani kao P. c. subsp. carotovorum (VR808, Brooke), P. brasiliense (Brooke) i P. versatile (VR808), prisutni u kombinovanoj infekciji. Svi izolati prikupljeni 2021. pripadaju vrsti P. brasiliense (VR808, Kiebitz, Brooke, Pirol, nepoznata sorta). Pripadnost ovim vrstama potvrđena je i filogenetskom analizom. Najnovija istraživanja crne noge krompira ukazuju da je došlo do izmene u populaciji prouzrokovača bolesti i da je trenutno bakterija P. brasiliense dominantna i sve prisutnija vrsta u Vojvodini.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021",
title = "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI"
}

Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović Milovanović, T.. (2021). NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021
Društvo za zaštitu bilja Srbije..

Marković S, Stanković S, Jelušić A, Iličić R, Popović Milovanović T. NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI. in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021. 2021;..

Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović Milovanović, Tatjana, "NOVIJA PROUČAVANJA CRNE NOGE KROMPIRA U SRBIJI" in XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor  22-25. novembar 2021 (2021).

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Milovanović, Predrag
AU  - Marković, Sanja
AU  - Blagojević, Milan
AU  - Iličić, Renata
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/796
AB  - Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I (Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa različitih kontinenata i biljnih domaćina. U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze (PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i Španije). Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni genotip bakterije.
PB  - Društvo za zaštitu bilja Srbije
C3  - . XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor, 22-25. novembar 2021. 44-44
T1  - DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA BRESKVE I KAJSIJE U SRBIJI
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Milovanović, Predrag and Marković, Sanja and Blagojević, Milan and Iličić, Renata",
year = "2021",
abstract = "Xanthomonas arboricola pv. pruni (Xap), prouzrokovač bakteriozne pegavosti listova i plodova, rak-rana i sušenja grana koštičavih voćnih vrsta, široko je rasprostranjena fitopatogena bakterija u svetu. Zbog šteta koje izaziva svrstana je u regulisane nekarantinske organizme u Evropi (EU_2019_2072_AnnexIV_RNQP), dok se u Srbiji nalazi na Listi IA deo I (Službeni glasnik 57/15). Parazitira vrste iz roda Prunus, a glavni domaćini od ekonomskog značaja su P. persica (breskva), P. persica var. nucipersica (nektarina), P. domestica (šljiva), P. armeniaca (kajsija) i P. dulcis (badem). U Srbiji je prvi put eksperimentalno potvrđena na području Fruške Gore i to na listovima breskve (Irig) tokom 2019. godine, a nakon toga 2020. na listovima i plodovima kajsije (Bešenovo). U ranijem periodu prisustvo Xap je potvrđeno u gotovo svim državama sa kojima se Srbija graniči. Cilj ovog rada je da se utvrdi genetički haplotip sojeva Xap izolovanih u Srbiji i uporedi sa populacijama ove vrste poreklom sa različitih kontinenata i biljnih domaćina. U radu je korišćeno 20 sojeva bakterije, 10 poreklom sa lista breskve izolovanih 2019. i 10 poreklom sa ploda kajsije izolovanih 2020. godine. Metoda lančane reakcije polimeraze (PCR) je korišćena za amplifikaciju dnaK, fyuA, gyrB i rpoD konzervisanih gena. Sekvenciranjem PCR produkata dobijene su parcijalne sekvence gena. Konkatamerna sekvenca (2558 nt) napravljena je spajanjem sekvenci 4 korišćena gena i korišćena za pravljenje haplotipske mreže (TCS algoritam implementiran u PopART v 1.7 programu) zajedno sa sojevima deponovanim u NCBI bazi podataka (17 sojeva Xap poreklom iz SAD, Novog Zelanda, Argentine, Australije, Brazila, Urugvaja, Južne Koreje, Francuske, Italije i Španije). Prema dobijenim rezultatima svi Xap sojevi poreklom iz Srbije (20) genetički su homogeni. Svi korišćeni Xap sojevi (37) razvrstani su u dva haplotipa; Xap sojevi poreklom iz Srbije kao i sojevi iz SAD, Italije, Francuske, Španije Australije i Brazila pripadaju Haplotipu I, dok sojevi poreklom iz Novog Zelanda, Argentine, Urugvaja i Južne Koreje pripadaju Haplotipu II. Rezultati ukazuju na postojanje dve genitički različite populacije ove fitopatogene bakterije. Takođe može se istaći da nije utvrđena korelacija u srodnosti i poreklu kao i distribuciji Xap, obzirom da mesto izolacije i domaćin sa koga su sojevi izolovani nisu uticali na utvrđeni genotip bakterije.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = ". XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor, 22-25. novembar 2021. 44-44",
title = "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA BRESKVE I KAJSIJE U SRBIJI"
}

Popović Milovanović, T., Jelušić, A., Milovanović, P., Marković, S., Blagojević, M.,& Iličić, R.. (2021). DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA BRESKVE I KAJSIJE U SRBIJI. in . XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor, 22-25. novembar 2021. 44-44
Društvo za zaštitu bilja Srbije..

Popović Milovanović T, Jelušić A, Milovanović P, Marković S, Blagojević M, Iličić R. DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA BRESKVE I KAJSIJE U SRBIJI. in . XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor, 22-25. novembar 2021. 44-44. 2021;..

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Milovanović, Predrag, Marković, Sanja, Blagojević, Milan, Iličić, Renata, "DETERMINACIJA HAPLOTIPA SOJEVA Xanthomonas arboricola pv. pruni POREKLOM SA BRESKVE I KAJSIJE U SRBIJI" in . XVI SIMPOZIJUM O ZAŠTITI BILJA Zbornik rezimea radova, Zlatibor, 22-25. novembar 2021. 44-44 (2021).

TY  - JOUR
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajić, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/674
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije
T2  - Genetika
T1  - Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Iličić, Renata and Popović Milovanović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajić, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije",
journal = "Genetika",
title = "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Iličić, R., Popović Milovanović, T., Marković, S., Jelušić, A., Bagi, F., Vlajić, S.,& Stanković, S.. (2021). Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika
Društvo genetičara Srbije., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Iličić R, Popović Milovanović T, Marković S, Jelušić A, Bagi F, Vlajić S, Stanković S. Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Iličić, Renata, Popović Milovanović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajić, Slobodan, Stanković, Slaviša, "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Poštić, Dobrivoj
AU  - Štrbanović, Ratibor
AU  - Broćić, Zoran
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Stanisavljević, Rade
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/687
AB  - The aim of this study was to determine the influence of the origin and size of planting material on the morphological
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40
and 60 g, compared to the larger fraction of 80 g.
PB  - Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi
T2  - Journal on Processing and Energy in Agriculture
T1  - Influence of origin and size of potato planting material on morphological characteristics of seed tubers
EP  - 23
IS  - 1
SP  - 20
VL  - 25
DO  - 10.5937/jpea25-30772
DO  - 2956-0195
ER  - 

@article{
author = "Poštić, Dobrivoj and Štrbanović, Ratibor and Broćić, Zoran and Popović Milovanović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Stanisavljević, Rade",
year = "2021",
abstract = "The aim of this study was to determine the influence of the origin and size of planting material on the morphological
characteristics of seed tuber's three potato cultivars. The objects of research were three varieties of potatoes: Aladdin, Newton and
Desiree. The analysis of the morphological characteristics tuber showed highly significant (р<0.01) differences under influenced
origin planting material (factor O) and size of seed tuber (factor S). Significance influence (р<0.01) genotype (factor G) was detected
on the number of sprouts per tuber and sprouts length. Seed tubers originating from a lower altitude (350 m a.s.l.) formed a
significantly (р<0.01) greater number of sprout per tuber, 0.59 or 7.33% more in comparison with tubers originating from 1300 m
a.s.l. Statistical analysis of the number of sprouts per tuber showed a significantly (р<0.01) lower number of germs in fractions of 40
and 60 g, compared to the larger fraction of 80 g.",
publisher = "Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi",
journal = "Journal on Processing and Energy in Agriculture",
title = "Influence of origin and size of potato planting material on morphological characteristics of seed tubers",
pages = "23-20",
number = "1",
volume = "25",
doi = "10.5937/jpea25-30772, 2956-0195"
}

Poštić, D., Štrbanović, R., Broćić, Z., Popović Milovanović, T., Marković, S., Jelušić, A.,& Stanisavljević, R.. (2021). Influence of origin and size of potato planting material on morphological characteristics of seed tubers. in Journal on Processing and Energy in Agriculture
Novi Sad : Nacionalno društvo za procesnu tehniku i energetiku u poljoprivredi., 25(1), 20-23.
https://doi.org/10.5937/jpea25-30772

Poštić D, Štrbanović R, Broćić Z, Popović Milovanović T, Marković S, Jelušić A, Stanisavljević R. Influence of origin and size of potato planting material on morphological characteristics of seed tubers. in Journal on Processing and Energy in Agriculture. 2021;25(1):20-23.
doi:10.5937/jpea25-30772 .

Poštić, Dobrivoj, Štrbanović, Ratibor, Broćić, Zoran, Popović Milovanović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Stanisavljević, Rade, "Influence of origin and size of potato planting material on morphological characteristics of seed tubers" in Journal on Processing and Energy in Agriculture, 25, no. 1 (2021):20-23,
https://doi.org/10.5937/jpea25-30772 . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Iličić, Renata
AU  - Veljović-Jovanović, Sonja
AU  - Milić Komić, Sonja
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/740
AB  - Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.
PB  - British Society for Plant Pathology
T2  - Plant Pathology
T1  - Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers
EP  - 1959
IS  - 8
SP  - 1945
VL  - 70
DO  - 10.1111/ppa.13421
DO  - 0032-0862
ER  - 

@article{
author = "Marković, Sanja and Stanković, Slaviša and Iličić, Renata and Veljović-Jovanović, Sonja and Milić Komić, Sonja and Jelušić, Aleksandra and Popović Milovanović, Tatjana",
year = "2021",
abstract = "Since 2011, the outbreaks of brown rot caused by Ralstonia solanacearum race 3, biovar 2, phylotype IIB-1 (R3/B2/PIIB-1) have significantly compromised potato production in Serbia. During 6 years of monitoring (2013–2018) among 3,524 potato tuber samples, 344 were found positive for brown rot disease. R. solanacearum R3/B2/PIIB-1 was isolated from seven cultivars among 12 monitored, and in five localities among 17 monitored. Cultivar Lady Claire was found to have the highest disease frequency (31.98%). A total of 78 isolates were identified by R. solanacearum-specific primer pairs (PS-1/PS-2 and OLI-1/Y-2), as well as the following tests: restriction fragment length polymorphism analysis, biovar determination, immunofluorescence, biochemical analysis, and pathogenicity. The genetic composition of 36 selected isolates assessed using multilocus sequence analysis with seven genes (adk, gapA, gdhA, gyrB, ppsA, hrpB, and fliC) showed that all isolates originating from Serbian potato were homogeneous. By using the TCS algorithm of concatenated sequences to get insight into the phylogeography of isolates and other R. solanacearum strains deposited in the NCBI database, we showed that their origin is undetermined. Peroxidase (POD) activity was measured in brown rotted potato tubers. A positive correlation was found between POD activity and disease severity rated on the analysed tubers. In general, POD activity increased by 2–22 times in vascular necrotic tissues compared to non-necrotic ones, and depended on disease severity but not on cultivar. Native polyacrylamide gel electrophoresis analysis of POD profiles resulted in a total of 10 distinct POD isoforms, of which PODs 3–5 were highly intensified in response to R. solanacearum.",
publisher = "British Society for Plant Pathology",
journal = "Plant Pathology",
title = "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers",
pages = "1959-1945",
number = "8",
volume = "70",
doi = "10.1111/ppa.13421, 0032-0862"
}

Marković, S., Stanković, S., Iličić, R., Veljović-Jovanović, S., Milić Komić, S., Jelušić, A.,& Popović Milovanović, T.. (2021). Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology
British Society for Plant Pathology., 70(8), 1945-1959.
https://doi.org/10.1111/ppa.13421

Marković S, Stanković S, Iličić R, Veljović-Jovanović S, Milić Komić S, Jelušić A, Popović Milovanović T. Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers. in Plant Pathology. 2021;70(8):1945-1959.
doi:10.1111/ppa.13421 .

Marković, Sanja, Stanković, Slaviša, Iličić, Renata, Veljović-Jovanović, Sonja, Milić Komić, Sonja, Jelušić, Aleksandra, Popović Milovanović, Tatjana, "Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers" in Plant Pathology, 70, no. 8 (2021):1945-1959,
https://doi.org/10.1111/ppa.13421 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/813
AB  - Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environmentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. isolates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology, 28-31.10.2020 133-133
T1  - In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović Milovanović, Tatjana",
year = "2020",
abstract = "Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environmentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. isolates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology, 28-31.10.2020 133-133",
title = "In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia"
}

Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović Milovanović, T.. (2020). In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia. in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..

Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović Milovanović T. In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia. in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133. 2020;..

Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović Milovanović, Tatjana, "In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia" in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133 (2020).

TY  - CONF
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/784
AB  - Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pectobacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides are not enough effective, therefore biological agents in their control are emerging as a possible solution. Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis). Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis (strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and dropplate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was performed on microtitre plates and measured by multi-well plate readers. Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 mm). The biofilm were formed in poor formation for all tested pathogens (category 1).
C3  - FEMS Online Conference on Microbiology 2020 28 - 31 October
T1  - Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens
SP  - 178
ER  - 

@conference{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2020",
abstract = "Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pectobacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides are not enough effective, therefore biological agents in their control are emerging as a possible solution. Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis). Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis (strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and dropplate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was performed on microtitre plates and measured by multi-well plate readers. Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 mm). The biofilm were formed in poor formation for all tested pathogens (category 1).",
journal = "FEMS Online Conference on Microbiology 2020 28 - 31 October",
title = "Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens",
pages = "178"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2020). Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens. in FEMS Online Conference on Microbiology 2020 28 - 31 October, 178.

Marković S, Popović Milovanović T, Jelušić A, Iličić R, Stanković S. Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens. in FEMS Online Conference on Microbiology 2020 28 - 31 October. 2020;:178..

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens" in FEMS Online Conference on Microbiology 2020 28 - 31 October (2020):178.

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/774
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins.
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes.
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced.
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R,
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chromatography-
mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp.
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies, Belgrade, Serbia
C3  - FEMS Online Conference on Microbiology 2020 28 – 31 October Electronic Abstract Book
T1  - PCR screening and chemical analysis of lipopeptides produced by Bacillus velezensis and Bacillus megaterium strains
SP  - 134
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins.
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes.
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced.
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R,
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chromatography-
mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp.
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies, Belgrade, Serbia",
journal = "FEMS Online Conference on Microbiology 2020 28 – 31 October Electronic Abstract Book",
title = "PCR screening and chemical analysis of lipopeptides produced by Bacillus velezensis and Bacillus megaterium strains",
pages = "134"
}

Jelušić, A., Popović Milovanović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR screening and chemical analysis of lipopeptides produced by Bacillus velezensis and Bacillus megaterium strains. in FEMS Online Conference on Microbiology 2020 28 – 31 October Electronic Abstract Book
Federation of European Microbiological Societies, Belgrade, Serbia., 134.

Jelušić A, Popović Milovanović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR screening and chemical analysis of lipopeptides produced by Bacillus velezensis and Bacillus megaterium strains. in FEMS Online Conference on Microbiology 2020 28 – 31 October Electronic Abstract Book. 2020;:134..

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR screening and chemical analysis of lipopeptides produced by Bacillus velezensis and Bacillus megaterium strains" in FEMS Online Conference on Microbiology 2020 28 – 31 October Electronic Abstract Book (2020):134.

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/804
AB  - Transmission of plant pathogens and pests across country borders has raised an alert in the EU. Some of them having a severe economic impact, leading for taking precautionary measures such as quarantine of plant materials transported across borders. Clavibacter michiganensis subsp. sepedonicus (Cms) is listed as a quarantine organism in EU. This bacterium causes potato ring rot disease and presents a worldwide threat due to crop losses during vegetation and in storage. Potato tubers from import in Serbia were tested for its presence in accordance with the official EU Council Directive. From the stolon end of 200 tubers (consist one sample), the small core of tissue containing vascular tissue was removed and the heel ends were taken and crashed in sterile phosphate buffer, then centrifuged. Re suspended pellet was used for immunofluorescence (IF, Loewe Biochemica GmbH), isolation of bacteria and DNA extraction for Polymerase Chain Reaction (PCR) performed with a pathogen-specific primer set PSA-1/PSA-R. As a positive control Cms reference strain CFBP 3561 was used. Two samples of ware potato originated from Belarus and Russian Federation in 2019 gave positive results for the presence of Cms. Visible internal symptom was observed on several tubers, in form of the vascular ring of tuber when they cut transversely. Bacterial ooze stream when tubers squeezed. The isolation of bacteria was performed from the ooze on Nutrient dextrose agar. Creamy-white, smooth colonies were formed after 3-5 days of incubation at 22 ºC. Restriction fragment length polymorphism (RFLP) of PCR products performed with PSA-1/PSA-R primer pair (502 bp) with enzyme BglII (fragments 282 and 220 bp in size) confirmed that the isolates belong to Cms. Pathogenicity was confirmed on aubergine seedlings, showing typical wilting obtained within 15-20 days after inoculation. Serbia is still free area from Cms pathogen due to rejection of contaminated potatoes from import.
PB  - Univerisity of East Sarajevo, Faculty of Agriculture
C3  - Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” 8-9 October 2020: 541-546
T1  - Risk of introduction of quarantine organisms: case Clavibacter michiganensis subsp. sepedonicus
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Marković, Sanja",
year = "2020",
abstract = "Transmission of plant pathogens and pests across country borders has raised an alert in the EU. Some of them having a severe economic impact, leading for taking precautionary measures such as quarantine of plant materials transported across borders. Clavibacter michiganensis subsp. sepedonicus (Cms) is listed as a quarantine organism in EU. This bacterium causes potato ring rot disease and presents a worldwide threat due to crop losses during vegetation and in storage. Potato tubers from import in Serbia were tested for its presence in accordance with the official EU Council Directive. From the stolon end of 200 tubers (consist one sample), the small core of tissue containing vascular tissue was removed and the heel ends were taken and crashed in sterile phosphate buffer, then centrifuged. Re suspended pellet was used for immunofluorescence (IF, Loewe Biochemica GmbH), isolation of bacteria and DNA extraction for Polymerase Chain Reaction (PCR) performed with a pathogen-specific primer set PSA-1/PSA-R. As a positive control Cms reference strain CFBP 3561 was used. Two samples of ware potato originated from Belarus and Russian Federation in 2019 gave positive results for the presence of Cms. Visible internal symptom was observed on several tubers, in form of the vascular ring of tuber when they cut transversely. Bacterial ooze stream when tubers squeezed. The isolation of bacteria was performed from the ooze on Nutrient dextrose agar. Creamy-white, smooth colonies were formed after 3-5 days of incubation at 22 ºC. Restriction fragment length polymorphism (RFLP) of PCR products performed with PSA-1/PSA-R primer pair (502 bp) with enzyme BglII (fragments 282 and 220 bp in size) confirmed that the isolates belong to Cms. Pathogenicity was confirmed on aubergine seedlings, showing typical wilting obtained within 15-20 days after inoculation. Serbia is still free area from Cms pathogen due to rejection of contaminated potatoes from import.",
publisher = "Univerisity of East Sarajevo, Faculty of Agriculture",
journal = "Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” 8-9 October 2020: 541-546",
title = "Risk of introduction of quarantine organisms: case Clavibacter michiganensis subsp. sepedonicus"
}

Popović Milovanović, T.,& Marković, S.. (2020). Risk of introduction of quarantine organisms: case Clavibacter michiganensis subsp. sepedonicus. in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” 8-9 October 2020: 541-546
Univerisity of East Sarajevo, Faculty of Agriculture..

Popović Milovanović T, Marković S. Risk of introduction of quarantine organisms: case Clavibacter michiganensis subsp. sepedonicus. in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” 8-9 October 2020: 541-546. 2020;..

Popović Milovanović, Tatjana, Marković, Sanja, "Risk of introduction of quarantine organisms: case Clavibacter michiganensis subsp. sepedonicus" in Proceedings of the XI International Scientific Agricultural Symposium “Agrosym 2020” 8-9 October 2020: 541-546 (2020).

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/816
AB  - Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins.
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes.
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced.
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R,
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chromatography-
mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp.
Presence of these molecules was also confirmed with the LC-MS.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology 28-31.10.2020 134-134
T1  - PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS VALEZENSIS AND BACILLUS MEGATERIUM STRAINS
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2020",
abstract = "Species belonging to the genus Bacillus are known for their ability to suppress various plant pathogenic fungi and
bacteria, due to synthesis of secondary metabolites like antibiotics and lipopeptides. The main lipopeptide families
responsible for the antimicrobial activity of Bacillus species are fengycins, iturins, kurstakins and surfactins.
Objectives:
The aim of this study was to identify and test two Bacillus-like isolates which showed in vitro antagonistic potential
against phytopathogenic bacterium Xanthomonas campestris pv. campestris for the presence of lipopeptide genes.
Methods:
For identification, DNA of tested isolates was amplified with primers based on 16S rRNA and tuf genes and sequenced.
The presence of lipopeptide genes was detected using primer pairs ITUP1-F/ITUP1-R, AKS-F/TKS-R, FEND1-F/FEND1-R,
BACC1-F/BACC1 and P17/P18 for detection of the genes for iturin, kurstakin, fengycin and bacillomycin D synthetases
and surfactin, respectively. The obtained PCR products were checked for correct size and sequenced. The liquid chromatography-
mass spectrometry (LC-MS) was used to confirm presence of the searched lipopeptides.
Results:
Based on the obtained sequences for 16S rRNA and tuf gene, isolates were identified as Bacillus valezensis and Bacillus
megaterium. The results of PCR screening showed that both strains have genes for biosynthesis of kurstakines and
surfactines, based on the obtained band of correct size (1125–1175 bp and 675 bp, respectively). B. valezensis strain
showed presence of the gene for bacillomycin D synthetase, producing the band on the position of 800 bp.
Presence of these molecules was also confirmed with the LC-MS.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology 28-31.10.2020 134-134",
title = "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS VALEZENSIS AND BACILLUS MEGATERIUM STRAINS"
}

Jelušić, A., Popović Milovanović, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Berić, T.. (2020). PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology 28-31.10.2020 134-134
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..

Jelušić A, Popović Milovanović T, Dimkić I, Mitrović P, Stanković S, Marković S, Berić T. PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS VALEZENSIS AND BACILLUS MEGATERIUM STRAINS. in FEMS Online Conference on Microbiology 28-31.10.2020 134-134. 2020;..

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "PCR SCREENING AND CHEMICAL ANALYSIS OF LIPOPEPTIDES PRODUCED BY BACILLUS VALEZENSIS AND BACILLUS MEGATERIUM STRAINS" in FEMS Online Conference on Microbiology 28-31.10.2020 134-134 (2020).

TY  - CONF
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/790
AB  - In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123
T1  - First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia
ER  - 

@conference{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2020",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123",
title = "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia"
}

Marković, S., Popović Milovanović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2020). First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini..

Marković S, Popović Milovanović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123. 2020;..

Marković, Sanja, Popović Milovanović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia" in 14th International Conference on Plant Pathogenic Bacteria,  7-12 June 2020, 123-123 (2020).

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/606
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected.
AB  - Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage
T1  - Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected., Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage, Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/913
AB  - The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, which produced characteristic pits on CVP medium were taken as representative. They were gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. carotovorum. Further genetic characterization was based on sequence analysis of the gapA and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 (KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity among the cabbage isolates.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2  - Pesticidi i fitomedicina
T1  - Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina
EP  - 222
IS  - 3-4
SP  - 211
VL  - 34
DO  - 10.2298/pif1904211p
DO  - 1820-3949
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Jelušić, Aleksandra and Marković, Sanja and Iličić, Renata",
year = "2019",
abstract = "The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King’s B and crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, which produced characteristic pits on CVP medium were taken as representative. They were gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent on King’s B medium, levan and arginine dehydrolase negative. The isolates were able to cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. carotovorum. Further genetic characterization was based on sequence analysis of the gapA and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 (KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity among the cabbage isolates.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina",
pages = "222-211",
number = "3-4",
volume = "34",
doi = "10.2298/pif1904211p, 1820-3949"
}

Popović Milovanović, T., Jelušić, A., Marković, S.,& Iličić, R.. (2019). Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 34(3-4), 211-222.
https://doi.org/10.2298/pif1904211p

Popović Milovanović T, Jelušić A, Marković S, Iličić R. Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina. in Pesticidi i fitomedicina. 2019;34(3-4):211-222.
doi:10.2298/pif1904211p .

Popović Milovanović, Tatjana, Jelušić, Aleksandra, Marković, Sanja, Iličić, Renata, "Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina" in Pesticidi i fitomedicina, 34, no. 3-4 (2019):211-222,
https://doi.org/10.2298/pif1904211p . .