TY  - JOUR
AU  - Nikolić, I.
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Popović, Tatjana
AU  - Lozo, J.
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/577
AB  - Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.
PB  - Hoboken : Wiley
T2  - Journal of Applied Microbiology
T1  - Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains
EP  - 176
IS  - 1
SP  - 165
VL  - 126
DO  - 10.1111/jam.14070
ER  - 

@article{
author = "Nikolić, I. and Berić, Tanja and Dimkić, Ivica and Popović, Tatjana and Lozo, J. and Fira, Đorđe and Stanković, Slaviša",
year = "2019",
abstract = "Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.",
publisher = "Hoboken : Wiley",
journal = "Journal of Applied Microbiology",
title = "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains",
pages = "176-165",
number = "1",
volume = "126",
doi = "10.1111/jam.14070"
}

Nikolić, I., Berić, T., Dimkić, I., Popović, T., Lozo, J., Fira, Đ.,& Stanković, S.. (2019). Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology
Hoboken : Wiley., 126(1), 165-176.
https://doi.org/10.1111/jam.14070

Nikolić I, Berić T, Dimkić I, Popović T, Lozo J, Fira Đ, Stanković S. Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology. 2019;126(1):165-176.
doi:10.1111/jam.14070 .

Nikolić, I., Berić, Tanja, Dimkić, Ivica, Popović, Tatjana, Lozo, J., Fira, Đorđe, Stanković, Slaviša, "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains" in Journal of Applied Microbiology, 126, no. 1 (2019):165-176,
https://doi.org/10.1111/jam.14070 . .

TY  - JOUR
AU  - Nikolić, I.
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Stojšin, Vera
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/553
AB  - Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet
EP  - 1207
IS  - 5
SP  - 1194
VL  - 67
DO  - 10.1111/ppa.12831
ER  - 

@article{
author = "Nikolić, I. and Stanković, Slaviša and Dimkić, Ivica and Berić, Tanja and Stojšin, Vera and Janse, Jaap and Popović, Tatjana",
year = "2018",
abstract = "Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet",
pages = "1207-1194",
number = "5",
volume = "67",
doi = "10.1111/ppa.12831"
}

Nikolić, I., Stanković, S., Dimkić, I., Berić, T., Stojšin, V., Janse, J.,& Popović, T.. (2018). Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology
Wiley, Hoboken., 67(5), 1194-1207.
https://doi.org/10.1111/ppa.12831

Nikolić I, Stanković S, Dimkić I, Berić T, Stojšin V, Janse J, Popović T. Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology. 2018;67(5):1194-1207.
doi:10.1111/ppa.12831 .

Nikolić, I., Stanković, Slaviša, Dimkić, Ivica, Berić, Tanja, Stojšin, Vera, Janse, Jaap, Popović, Tatjana, "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet" in Plant Pathology, 67, no. 5 (2018):1194-1207,
https://doi.org/10.1111/ppa.12831 . .