TY  - JOUR
AU  - Nikolić, I.
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Stojšin, Vera
AU  - Janse, Jaap
AU  - Popović, Tatjana
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/553
AB  - Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet
EP  - 1207
IS  - 5
SP  - 1194
VL  - 67
DO  - 10.1111/ppa.12831
ER  - 

@article{
author = "Nikolić, I. and Stanković, Slaviša and Dimkić, Ivica and Berić, Tanja and Stojšin, Vera and Janse, Jaap and Popović, Tatjana",
year = "2018",
abstract = "Pseudomonas syringae pv. aptata is the causal agent of bacterial leaf spot disease of sugar beet (Beta vulgaris). During 2013, 250 samples were collected from leaf lesions with typical symptoms of bacterial leaf spot in commercial fields of sugar beet in Serbia, and 104 isolates of P.syringae pv. aptata were obtained. Identification and characterization was performed using biochemical, molecular and pathogenicity tests. Identification included LOPAT tests and positive reactions using primers Papt2F and Papt1R specific for P.syringae pv. aptata. Repetitive (rep) sequence-based PCR typing with ERIC, REP and BOX primers revealed high genetic variability among isolates and distinguished 25 groups of different fingerprinting profiles. Pulse-field gel electrophoresis (PFGE) and multilocus sequence analysis (MLSA) of representative isolates showed higher genetic variability than in rep-PCR analysis and distinguished three and four major genetic clusters, respectively. A pathogenicity test performed with 25 representative isolates on four cultivars of sugar beet confirmed the occurrence of leaf spot disease and showed correlation between the most aggressive isolates and the genetic clusters obtained in MLSA. All these findings point to the existence of several lines of P.syringae pv. aptata infection in Serbia that are genetically and pathologically different.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet",
pages = "1207-1194",
number = "5",
volume = "67",
doi = "10.1111/ppa.12831"
}

Nikolić, I., Stanković, S., Dimkić, I., Berić, T., Stojšin, V., Janse, J.,& Popović, T.. (2018). Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology
Wiley, Hoboken., 67(5), 1194-1207.
https://doi.org/10.1111/ppa.12831

Nikolić I, Stanković S, Dimkić I, Berić T, Stojšin V, Janse J, Popović T. Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet. in Plant Pathology. 2018;67(5):1194-1207.
doi:10.1111/ppa.12831 .

Nikolić, I., Stanković, Slaviša, Dimkić, Ivica, Berić, Tanja, Stojšin, Vera, Janse, Jaap, Popović, Tatjana, "Genetic diversity and pathogenicity of Pseudomonas syringae pv. aptata isolated from sugar beet" in Plant Pathology, 67, no. 5 (2018):1194-1207,
https://doi.org/10.1111/ppa.12831 . .

TY  - JOUR
AU  - Radunović, Dragana
AU  - Gavrilović, Veljko
AU  - Gašić, Katarina
AU  - Paunović, M.
AU  - Stojšin, Vera
AU  - Grahovac, M.
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/484
AB  - In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.
PB  - Edizioni Ets, Pisa
T2  - Journal of Plant Pathology
T1  - Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro
EP  - 203
IS  - 1
SP  - 197
VL  - 99
DO  - 10.4454/jpp.v99i1.3853
ER  - 

@article{
author = "Radunović, Dragana and Gavrilović, Veljko and Gašić, Katarina and Paunović, M. and Stojšin, Vera and Grahovac, M.",
year = "2017",
abstract = "In the period from 2012-2015 plant samples with fireblight symptoms were collected from pome fruits and indigenous plant, in main fruitgrowing regions of Montenegro. After succesfull isolation, pathogenicity of the obtained strains was tested by artificial inoculation of immature pear fruits, variety Viljamovka. Hypersensitive reaction was tested on tobacco leaves, variety White Burley. Identification and genetic diversity studies were performed using several molecular techniques on 18 Erwinia amylovora strains originating from quince, pear, apple and hawthorn. Bacterial identity was confirmed by nested PCR in which all studied strains produced the expected amplification fragment of plasmid pEA29. To detect potential genetic variations in E. amylovora population, rep-PCR was conducted. Using REP, ERIC and BOX primers, in all three PCR reactions, differences between studied strains were detected, i.e. pear strains had different genetic profiles from all other studied strains, including reference strain. Genetic variability of selected E. amylovora strains was studied by RAPD-PCR as well. Both of the used random primers, CUGEA-3 and CUGEA-5, showed discriminatory potential by separating genetic profiles of pear strains from all other studied strains, including reference strain. This is the first study of genetic variability of E. amylovora in Montenegro.",
publisher = "Edizioni Ets, Pisa",
journal = "Journal of Plant Pathology",
title = "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro",
pages = "203-197",
number = "1",
volume = "99",
doi = "10.4454/jpp.v99i1.3853"
}

Radunović, D., Gavrilović, V., Gašić, K., Paunović, M., Stojšin, V.,& Grahovac, M.. (2017). Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology
Edizioni Ets, Pisa., 99(1), 197-203.
https://doi.org/10.4454/jpp.v99i1.3853

Radunović D, Gavrilović V, Gašić K, Paunović M, Stojšin V, Grahovac M. Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro. in Journal of Plant Pathology. 2017;99(1):197-203.
doi:10.4454/jpp.v99i1.3853 .

Radunović, Dragana, Gavrilović, Veljko, Gašić, Katarina, Paunović, M., Stojšin, Vera, Grahovac, M., "Molecular characterization of Erwinia Amylovora strains originated from pome fruits and indigenous plant in montenegro" in Journal of Plant Pathology, 99, no. 1 (2017):197-203,
https://doi.org/10.4454/jpp.v99i1.3853 . .

TY  - JOUR
AU  - Stojšin, Vera
AU  - Balaž, Jelica
AU  - Budakov, Dragana B.
AU  - Nikolić, Ivan
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/412
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia
EP  - 281
IS  - 2
SP  - 281
VL  - 99
DO  - 10.1094/PDIS-06-14-0628-PDN
ER  - 

@article{
author = "Stojšin, Vera and Balaž, Jelica and Budakov, Dragana B. and Nikolić, Ivan and Ivanović, Žarko and Popović, Tatjana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia",
pages = "281-281",
number = "2",
volume = "99",
doi = "10.1094/PDIS-06-14-0628-PDN"
}

Stojšin, V., Balaž, J., Budakov, D. B., Nikolić, I., Ivanović, Ž.,& Popović, T.. (2015). First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 281-281.
https://doi.org/10.1094/PDIS-06-14-0628-PDN

Stojšin V, Balaž J, Budakov DB, Nikolić I, Ivanović Ž, Popović T. First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia. in Plant Disease. 2015;99(2):281-281.
doi:10.1094/PDIS-06-14-0628-PDN .

Stojšin, Vera, Balaž, Jelica, Budakov, Dragana B., Nikolić, Ivan, Ivanović, Žarko, Popović, Tatjana, "First Report of Pseudomonas syringae pv. aptata Causing Bacterial Leaf Spot on Sugar Beet in Serbia" in Plant Disease, 99, no. 2 (2015):281-281,
https://doi.org/10.1094/PDIS-06-14-0628-PDN . .

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Jošić, Dragana
AU  - Starović, Mira
AU  - Stojanović, Saša
AU  - Aleksic, Goran
AU  - Stojšin, Vera
AU  - Radanović, Dragoja
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/891
AB  - The symptoms indicating phytoplasma like leaf yellowing, reddening and early drying were observed on two St. John’s worth species (Hypericum perforatum L. and Hypericum barbatum L.) on infected fields (Pancevo, Indjija and Stara Pazova) in Serbia in 2008. Electron microscopy examination of the ultra-thin sections revealed the presence of numerous polymorphic phytoplasma-like bodies in the phloem tissue of leaf midribs and petioles. The phytoplasma etiology was confirmed by polymerase chain reaction (PCR) using 3 sets of primers (P1/P7, P1/16S-Sr and R16F2n/R16R2). Restriction fragment length polymorphism (RFLP) analysis of amplification products of 1.2 kb (obtained with R16F2n/R2 primer pair) in 51 from 60 symptomatic plants, indicated the presence of 16SrXII-A phytoplasma subgroup from all three affected localities. Sequence of R16F2n/R2 amplicon for representative phytoplasma H. perforatum L. isolate Hp22 was deposited in the GeneBank with accession number JQ033928. This is the first report of the natural occurrence of Stolbur phytoplasma in two cultivated St. John’s worth species in Serbia.
PB  - Academic Journals
T2  - Journal of Medicinal Plants Research
T1  - The first Stolbur Phytoplasma occurrence on two St. John's Worth species (Hypericum perforatum L. and Hypericum barbatum L.) in Serbia
EP  - 911
IS  - 5
SP  - 906
VL  - 6
DO  - 10.5897/JMPR11.1584
ER  - 

@article{
author = "Pavlović, Snežana and Jošić, Dragana and Starović, Mira and Stojanović, Saša and Aleksic, Goran and Stojšin, Vera and Radanović, Dragoja",
year = "2012",
abstract = "The symptoms indicating phytoplasma like leaf yellowing, reddening and early drying were observed on two St. John’s worth species (Hypericum perforatum L. and Hypericum barbatum L.) on infected fields (Pancevo, Indjija and Stara Pazova) in Serbia in 2008. Electron microscopy examination of the ultra-thin sections revealed the presence of numerous polymorphic phytoplasma-like bodies in the phloem tissue of leaf midribs and petioles. The phytoplasma etiology was confirmed by polymerase chain reaction (PCR) using 3 sets of primers (P1/P7, P1/16S-Sr and R16F2n/R16R2). Restriction fragment length polymorphism (RFLP) analysis of amplification products of 1.2 kb (obtained with R16F2n/R2 primer pair) in 51 from 60 symptomatic plants, indicated the presence of 16SrXII-A phytoplasma subgroup from all three affected localities. Sequence of R16F2n/R2 amplicon for representative phytoplasma H. perforatum L. isolate Hp22 was deposited in the GeneBank with accession number JQ033928. This is the first report of the natural occurrence of Stolbur phytoplasma in two cultivated St. John’s worth species in Serbia.",
publisher = "Academic Journals",
journal = "Journal of Medicinal Plants Research",
title = "The first Stolbur Phytoplasma occurrence on two St. John's Worth species (Hypericum perforatum L. and Hypericum barbatum L.) in Serbia",
pages = "911-906",
number = "5",
volume = "6",
doi = "10.5897/JMPR11.1584"
}

Pavlović, S., Jošić, D., Starović, M., Stojanović, S., Aleksic, G., Stojšin, V.,& Radanović, D.. (2012). The first Stolbur Phytoplasma occurrence on two St. John's Worth species (Hypericum perforatum L. and Hypericum barbatum L.) in Serbia. in Journal of Medicinal Plants Research
Academic Journals., 6(5), 906-911.
https://doi.org/10.5897/JMPR11.1584

Pavlović S, Jošić D, Starović M, Stojanović S, Aleksic G, Stojšin V, Radanović D. The first Stolbur Phytoplasma occurrence on two St. John's Worth species (Hypericum perforatum L. and Hypericum barbatum L.) in Serbia. in Journal of Medicinal Plants Research. 2012;6(5):906-911.
doi:10.5897/JMPR11.1584 .

Pavlović, Snežana, Jošić, Dragana, Starović, Mira, Stojanović, Saša, Aleksic, Goran, Stojšin, Vera, Radanović, Dragoja, "The first Stolbur Phytoplasma occurrence on two St. John's Worth species (Hypericum perforatum L. and Hypericum barbatum L.) in Serbia" in Journal of Medicinal Plants Research, 6, no. 5 (2012):906-911,
https://doi.org/10.5897/JMPR11.1584 . .

TY  - JOUR
AU  - Pavlović, Snežana
AU  - Stojšin, Vera
AU  - Stojanović, Saša
AU  - Starović, Mira
AU  - Bagi, Ferenc F.
AU  - Budakov, Dragana B.
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/118
AB  - Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described.
AB  - Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) je konstatovana na belom slezu, ehinacei i kantarionu i to na semenu, korenu i stablu, kao i na rasadu ehinacea. Osnovni simptom zaraženih biljaka G. intermedia su bili: propadanje klijanaca, trulež korena i korenovog vrata, patuljavost, asimetričan porast, uvelost, hloroza i smežuranost listova. Uzorci zaraženog biljnog materijala bili su poreklom iz okoline Pančeva, Zrenjanina, Banatskog Novog Sela, Inðije, Rume i Stare Pazove. U radu su date morfološke, fiziološke i patogene karakteristike izolata G. intermedia.
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia
T1  - Gibberella intermedia patogen kantariona, ehinacea i belog sleza u Srbiji
EP  - 199
IS  - 116
SP  - 191
DO  - 10.2298/ZMSPN0916191P
ER  - 

@article{
author = "Pavlović, Snežana and Stojšin, Vera and Stojanović, Saša and Starović, Mira and Bagi, Ferenc F. and Budakov, Dragana B.",
year = "2009",
abstract = "Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) was isolated from seeds of St. John's wort, marshmallow, and coneflower, as well as from roots and stalks of marshmallow and roots of coneflower. These plants had symptoms of leaf chlorosis, malformation, withering and plant dwarfing and were collected from several localities in Serbia during five-year investigations of mycopopulations of the mentioned plants. The morphological characteristics of the pathogen were described., Gibberella intermedia (Kuhlmann) Samuels et al. (anamorf: Fusarium proliferatum /Matsushima/ Nirenberg) je konstatovana na belom slezu, ehinacei i kantarionu i to na semenu, korenu i stablu, kao i na rasadu ehinacea. Osnovni simptom zaraženih biljaka G. intermedia su bili: propadanje klijanaca, trulež korena i korenovog vrata, patuljavost, asimetričan porast, uvelost, hloroza i smežuranost listova. Uzorci zaraženog biljnog materijala bili su poreklom iz okoline Pančeva, Zrenjanina, Banatskog Novog Sela, Inðije, Rume i Stare Pazove. U radu su date morfološke, fiziološke i patogene karakteristike izolata G. intermedia.",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia, Gibberella intermedia patogen kantariona, ehinacea i belog sleza u Srbiji",
pages = "199-191",
number = "116",
doi = "10.2298/ZMSPN0916191P"
}

Pavlović, S., Stojšin, V., Stojanović, S., Starović, M., Bagi, F. F.,& Budakov, D. B.. (2009). Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(116), 191-199.
https://doi.org/10.2298/ZMSPN0916191P

Pavlović S, Stojšin V, Stojanović S, Starović M, Bagi FF, Budakov DB. Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia. in Zbornik Matice srpske za prirodne nauke. 2009;(116):191-199.
doi:10.2298/ZMSPN0916191P .

Pavlović, Snežana, Stojšin, Vera, Stojanović, Saša, Starović, Mira, Bagi, Ferenc F., Budakov, Dragana B., "Gibberella intermedia the pathogen of St. John's Wort, coneflower and marshmallow in Serbia" in Zbornik Matice srpske za prirodne nauke, no. 116 (2009):191-199,
https://doi.org/10.2298/ZMSPN0916191P . .

TY  - CONF
AU  - Pavlović, Snežana
AU  - Stojanović, S.
AU  - Starović, Mira
AU  - Stević, Tatjana
AU  - Stojšin, Vera
PY  - 2009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/117
PB  - Georg Thieme Verlag Kg, Stuttgart
C3  - Planta Medica
T1  - Mycopopulation in five important cultivated medicinal plants in Serbia
EP  - 1005
IS  - 9
SP  - 1005
VL  - 75
ER  - 

@conference{
author = "Pavlović, Snežana and Stojanović, S. and Starović, Mira and Stević, Tatjana and Stojšin, Vera",
year = "2009",
publisher = "Georg Thieme Verlag Kg, Stuttgart",
journal = "Planta Medica",
title = "Mycopopulation in five important cultivated medicinal plants in Serbia",
pages = "1005-1005",
number = "9",
volume = "75"
}

Pavlović, S., Stojanović, S., Starović, M., Stević, T.,& Stojšin, V.. (2009). Mycopopulation in five important cultivated medicinal plants in Serbia. in Planta Medica
Georg Thieme Verlag Kg, Stuttgart., 75(9), 1005-1005.

Pavlović S, Stojanović S, Starović M, Stević T, Stojšin V. Mycopopulation in five important cultivated medicinal plants in Serbia. in Planta Medica. 2009;75(9):1005-1005..

Pavlović, Snežana, Stojanović, S., Starović, Mira, Stević, Tatjana, Stojšin, Vera, "Mycopopulation in five important cultivated medicinal plants in Serbia" in Planta Medica, 75, no. 9 (2009):1005-1005.