TY  - CONF
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/522
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
ER  - 

@conference{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, M. and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133..

Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, M., Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133.

TY  - CONF
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/521
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
ER  - 

@conference{
author = "Prokić, Anđelka and Ivanović, M. and Gašić, Katarina and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24..

Prokić, Anđelka, Ivanović, M., Gašić, Katarina, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24.

TY  - CONF
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2016
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/447
AB  - Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - III Balkan Symposium on Fruit Growing
T1  - Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia
EP  - 719
SP  - 715
VL  - 1139
DO  - 10.17660/ActaHortic.2016.1139.122
ER  - 

@conference{
author = "Ivanović, M. and Gašić, Katarina and Prokić, Anđelka and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2016",
abstract = "Continuous use of copper-based treatments and antibiotics in pear and apple protection programs may result in appearance of copper or antibiotic resistant strains of Erwinia amylovora. In order to determine the potential occurrence of copper or/and antibiotic resistance in Serbia we studied in vitro effect of different concentrations of copper sulfate, streptomycin and kasugamycin on development and growth of 40 E. amylovora strains originating from this region. Filter sterilized solution of the bactericides was added to the NA medium after sterilization to a final concentration of 100 and 200 ppm, respectively, followed by spot inoculation of each strain on the medium. None of the strains developed on NA amended with 100 or 200 ppm of streptomycin or kasugamycin. Our study showed that, despite the potential exposure to the antibiotics, E amylovora strains from Serbia did not develop resistance to streptomycin or kasugamycin. On the other hand, all strains had normal growth on NA amended with 100 ppm of copper sulfate indicating certain level of resistance to copper ions. At 200 ppm, 28 strains formed colonies of normal size and appearance, while 12 strains had smaller colonies with reduced growth. This might be due to the extensive and frequent use of copper-based compounds to control fire blight in Serbia.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "III Balkan Symposium on Fruit Growing",
title = "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia",
pages = "719-715",
volume = "1139",
doi = "10.17660/ActaHortic.2016.1139.122"
}

Ivanović, M., Gašić, K., Prokić, A., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2016). Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing
Int Soc Horticultural Science, Leuven 1., 1139, 715-719.
https://doi.org/10.17660/ActaHortic.2016.1139.122

Ivanović M, Gašić K, Prokić A, Kuzmanović N, Zlatković N, Obradović A. Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia. in III Balkan Symposium on Fruit Growing. 2016;1139:715-719.
doi:10.17660/ActaHortic.2016.1139.122 .

Ivanović, M., Gašić, Katarina, Prokić, Anđelka, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Screening for copper and antibiotic resistance in Erwinia amylovora population from Serbia" in III Balkan Symposium on Fruit Growing, 1139 (2016):715-719,
https://doi.org/10.17660/ActaHortic.2016.1139.122 . .

TY  - CONF
AU  - Gašić, Katarina
AU  - Ivanović, M.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
AU  - Šević, Milan
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/311
AB  - In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XIII International Workshop on Fire Blight
T1  - Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential
EP  - 126
SP  - 123
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.17
ER  - 

@conference{
author = "Gašić, Katarina and Ivanović, M. and Kuzmanović, Nemanja and Prokić, Anđelka and Obradović, Aleksa and Šević, Milan",
year = "2014",
abstract = "In search for an alternative to chemical control of fire blight, we isolated seven bacteriophages specific to E. amylovora. Three phages were isolated from water, three from symptomless pear leaves and one from apple leaves with characteristic fire blight symptoms. Host-range studies showed that the phages differed in the ability to lyse 40 strains of E. amylovora isolated in Serbia. Based on its lytic activity, phage Phi Ea2 was chosen for further study of biological control potential in pear and apple blossom bioassays. A phage suspension of 10(8) PFU/ml was applied on blossoms either 2 h before, 2 h after or at the same time of inoculation. In three fully replicated bioassay experiments, application of host-specific phages 2 h before inoculation and at the time of inoculation significantly reduced fire blight symptom development compared to untreated control. However, disease reduction was not significant when phages were applied 2 h after inoculation.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential",
pages = "126-123",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.17"
}

Gašić, K., Ivanović, M., Kuzmanović, N., Prokić, A., Obradović, A.,& Šević, M.. (2014). Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 123-126.
https://doi.org/10.17660/ActaHortic.2014.1056.17

Gašić K, Ivanović M, Kuzmanović N, Prokić A, Obradović A, Šević M. Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential. in XIII International Workshop on Fire Blight. 2014;1056:123-126.
doi:10.17660/ActaHortic.2014.1056.17 .

Gašić, Katarina, Ivanović, M., Kuzmanović, Nemanja, Prokić, Anđelka, Obradović, Aleksa, Šević, Milan, "Bacteriophage of Erwinia amylovora - Host Range and Fire Blight Control Potential" in XIII International Workshop on Fire Blight, 1056 (2014):123-126,
https://doi.org/10.17660/ActaHortic.2014.1056.17 . .

TY  - CONF
AU  - Ivanović, M.
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Blagojević, Nevena
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/315
AB  - In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XIII International Workshop on Fire Blight
T1  - Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR
EP  - 84
SP  - 81
VL  - 1056
DO  - 10.17660/ActaHortic.2014.1056.10
ER  - 

@conference{
author = "Ivanović, M. and Kuzmanović, Nemanja and Prokić, Anđelka and Blagojević, Nevena and Obradović, Aleksa and Gašić, Katarina",
year = "2014",
abstract = "In this study, three bacterial DNA extraction procedures were compared prior to real-time PCR. Healthy pear leaves and twigs were crushed in antioxidant maceration buffer and spiked with Erwinia amylovora to final concentrations from 2.1 x 10(6) to 2.1 x 10(1) cells ml(-1). Bacterial DNA was extracted from aliquots of spiked crude extracts using (i) isopropanol, (ii) REDExtract-N-Amp (TM) Plant PCR kit, and (iii) Taylor's modified DNA purification procedure. The ams region of the chromosomal DNA was selected as target for the real-time PCR. In this study, the REDExtract-N-Amp (TM) and Taylor's modified DNA extraction procedure were most successful in removing PCR inhibitors, leading to detection of 2.1x10(2) E. amylovora CFU/ml. At this concentration, pathogen can be efficiently detected in less than 5 h in spite of inhibitors and plant DNA reducing sensitivity of the reaction. These two methods increased amplification efficiency in real-time PCR compared to a simple isopropanol DNA extraction procedure from plant tissues, where the lowest detected concentration was 2.1 x 10(4) CFU/ml. In our research, real-time PCR has proven to be very sensitive method for detection of E. amylovora in plant material. It was 100 times more sensitive compared to other conventional PCR procedures.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XIII International Workshop on Fire Blight",
title = "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR",
pages = "84-81",
volume = "1056",
doi = "10.17660/ActaHortic.2014.1056.10"
}

Ivanović, M., Kuzmanović, N., Prokić, A., Blagojević, N., Obradović, A.,& Gašić, K.. (2014). Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in XIII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 1056, 81-84.
https://doi.org/10.17660/ActaHortic.2014.1056.10

Ivanović M, Kuzmanović N, Prokić A, Blagojević N, Obradović A, Gašić K. Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR. in XIII International Workshop on Fire Blight. 2014;1056:81-84.
doi:10.17660/ActaHortic.2014.1056.10 .

Ivanović, M., Kuzmanović, Nemanja, Prokić, Anđelka, Blagojević, Nevena, Obradović, Aleksa, Gašić, Katarina, "Evaluation of Three Extraction Methods for Detection of Erwinia amylovora from Pear Leaves by Real-Time PCR" in XIII International Workshop on Fire Blight, 1056 (2014):81-84,
https://doi.org/10.17660/ActaHortic.2014.1056.10 . .

TY  - CONF
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
AU  - Dickstein, Ellen R
AU  - Jones, J. B.
AU  - Gavrilović, Veljko
AU  - Balaž, Jelica
PY  - 2011
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/156
AB  - A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.
PB  - Int Soc Horticultural Science, Leuven 1
C3  - XII International Workshop on Fire Blight
T1  - Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG
EP  - 71
SP  - 65
VL  - 896
ER  - 

@conference{
author = "Ivanović, M. and Gašić, Katarina and Obradović, Aleksa and Dickstein, Ellen R and Jones, J. B. and Gavrilović, Veljko and Balaž, Jelica",
year = "2011",
abstract = "A collection of 41 Erwinia amylovora strains isolated from 8 plant species at 13 locations in Serbia and one location in Montenegro was tested and their relationship was determined based on carbohydrate metabolism and fatty acid composition. Metabolism of carbon compounds was tested using the Biolog GN MicroPlate system. After 24 h of incubation, the resulting metabolic fingerprints were recorded and compared with the Biolog GN database. Fatty acid composition was analyzed by Microbial Identification System and compared with the data from the MIDI database. Our results showed that all strains tested were identified as E. amylovora, with similarity index (SI) between 0.62 and 0.99, using BIOLOG. Based on fatty acid composition, 39 strains were identified as E. amylovora, with SI between 0.61 and 0.92. More than 14 fatty acids were detected, eight of which were present in all strains with more than 1% of the total named peak area. Based on quantitative and qualitative content of fatty acid composition, the isolates were clustered into three groups: alpha, beta and gamma (Euclidian distance  lt = 6). Cluster analysis revealed that groups beta and gamma consists of only strains isolated from north Serbia, whereas all strains isolated from central or south Serbia belong to group alpha.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "XII International Workshop on Fire Blight",
title = "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG",
pages = "71-65",
volume = "896"
}

Ivanović, M., Gašić, K., Obradović, A., Dickstein, E. R., Jones, J. B., Gavrilović, V.,& Balaž, J.. (2011). Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in XII International Workshop on Fire Blight
Int Soc Horticultural Science, Leuven 1., 896, 65-71.

Ivanović M, Gašić K, Obradović A, Dickstein ER, Jones JB, Gavrilović V, Balaž J. Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG. in XII International Workshop on Fire Blight. 2011;896:65-71..

Ivanović, M., Gašić, Katarina, Obradović, Aleksa, Dickstein, Ellen R, Jones, J. B., Gavrilović, Veljko, Balaž, Jelica, "Identification and Differentiation of Erwinia amylovora Using Fatty Acid Analysis and BIOLOG" in XII International Workshop on Fire Blight, 896 (2011):65-71.