TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Obradović, Aleksa
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/381
AB  - Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.
PB  - Amer Soc Microbiology, Washington
T2  - Microbiology Resource Announcements
T1  - Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330
IS  - 2
VL  - 3
DO  - 10.1128/genomeA.00331-15
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Anđelka and Ivanović, Milan and Zlatković, Nevena and Gašić, Katarina and Obradović, Aleksa",
year = "2015",
abstract = "Tumorigenic strains of Agrobacterium spp. are responsible for crown gall disease of numerous plant species. We present here draft genome sequences of nonpathogenic Agrobacterium nepotum strain 39/7(T) (CFBP 7436(T), LMG 26435(T)), isolated from crown gall tumor on Prunus cerasifera, and tumorigenic Agrobacterium sp. strain KFB 330 (CFBP 8308, LMG 28674), isolated from galls on raspberry.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Microbiology Resource Announcements",
title = "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330",
number = "2",
volume = "3",
doi = "10.1128/genomeA.00331-15"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Gašić, K.,& Obradović, A.. (2015). Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements
Amer Soc Microbiology, Washington., 3(2).
https://doi.org/10.1128/genomeA.00331-15

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Gašić K, Obradović A. Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330. in Microbiology Resource Announcements. 2015;3(2).
doi:10.1128/genomeA.00331-15 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Gašić, Katarina, Obradović, Aleksa, "Draft Genome Sequences of Agrobacterium nepotum Strain 39/7(T) and Agrobacterium sp. Strain KFB 330" in Microbiology Resource Announcements, 3, no. 2 (2015),
https://doi.org/10.1128/genomeA.00331-15 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan M
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Blagojević, Nevena
AU  - Pulawska, Joanna
AU  - Obradović, Aleksa
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/299
AB  - Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia
EP  - 16
IS  - 1
SP  - 11
VL  - 137
DO  - 10.1007/s10658-013-0229-0
ER  - 

@article{
author = "Kuzmanović, Nemanja and Ivanović, Milan M and Prokić, Anđelka and Gašić, Katarina and Blagojević, Nevena and Pulawska, Joanna and Obradović, Aleksa",
year = "2013",
abstract = "Crown gall, caused by tumorigenic strains of Agrobacterium spp., is considered one of the most important diseases in stone fruit nurseries throughout the world. Since the crown gall disease has not been studied extensively in Serbia for more than 30 years, the objective of this study was to isolate, identify and characterize the bacterium associated with crown gall symptoms on one-year-old apricot trees. Samples were collected from a nursery in central Serbia and subjected to laboratory analysis. Bacteria were isolated from tumour tissue on yeast mannitol agar (YMA) and six gram-negative isolates were selected for further study. PCR assay using primers specific for virD2, virC, ipt and tms2 pathogenicity-associated plasmid genes revealed that isolates harbour Ti plasmids. All studied strains carrying Ti plasmids were classified as nopaline-type based on further molecular analysis. Using a multiplex PCR assay, targeting 23S rRNA gene sequences, and physiological and biochemical tests, five strains were assigned as A. rhizogenes/biovar 2 and the remaining one as A. tumefaciens/biovar 1. Identity of the strains was confirmed by sequence analysis of the 16S rRNA gene. In pathogenicity assay, all six strains caused tumour formation on inoculated carrot root discs, young tomato and sunflower plants.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia",
pages = "16-11",
number = "1",
volume = "137",
doi = "10.1007/s10658-013-0229-0"
}

Kuzmanović, N., Ivanović, M. M., Prokić, A., Gašić, K., Blagojević, N., Pulawska, J.,& Obradović, A.. (2013). Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 137(1), 11-16.
https://doi.org/10.1007/s10658-013-0229-0

Kuzmanović N, Ivanović MM, Prokić A, Gašić K, Blagojević N, Pulawska J, Obradović A. Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia. in European Journal of Plant Pathology. 2013;137(1):11-16.
doi:10.1007/s10658-013-0229-0 .

Kuzmanović, Nemanja, Ivanović, Milan M, Prokić, Anđelka, Gašić, Katarina, Blagojević, Nevena, Pulawska, Joanna, Obradović, Aleksa, "Identification and characterization of Agrobacterium spp. isolated from apricot in Serbia" in European Journal of Plant Pathology, 137, no. 1 (2013):11-16,
https://doi.org/10.1007/s10658-013-0229-0 . .

Kuzmanović, N., Ćalić, A., Ivanović, M., Gašić, K., Pulawska, J.,& Obradović, A.. (2012). First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia. in Plant Disease
American Phytopathological Society., 96(2), 286.
https://doi.org/10.1094/PDIS-07-11-0600

Kuzmanović N, Ćalić A, Ivanović M, Gašić K, Pulawska J, Obradović A. First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia. in Plant Disease. 2012;96(2):286.
doi:10.1094/PDIS-07-11-0600 .

Kuzmanović, Nemanja, Ćalić, Anđelka, Ivanović, Milan, Gašić, Katarina, Pulawska, Joanna, Obradović, Aleksa, "First Report of Agrobacterium vitis as the Causal Agent of Grapevine Crown Gall in Serbia" in Plant Disease, 96, no. 2 (2012):286,
https://doi.org/10.1094/PDIS-07-11-0600 . .

TY  - JOUR
AU  - Kuzmanović, Nemanja
AU  - Pulawska, Joanna
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zlatković, Nevena
AU  - Jeffrey, Jones
AU  - Obradović, Aleksa
PY  - 201
UR  - https://enauka.gov.rs/handle/123456789/149425
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3716
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1187
AB  - Two plant-tumorigenic strains KFB 330T and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A. nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD, glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, “A. fabrum” and A. pusense. This was further supported by average nucleotide identity values (<92%) between the whole genome sequences of strain KFB 330T and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8–77.87%) and 16:0 (6.82–8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain of A. arsenijevicii is KFB 330T (=CFBP 8308T = LMG 28674T).
PB  - Elsevier
T2  - Systematic and Applied Microbiology
T1  - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
EP  - 378
IS  - 6
SP  - 373
VL  - 38
DO  - 10.1016/j.syapm.2015.06.001
ER  - 

@article{
author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Anđelka and Ivanović, Milan and Zlatković, Nevena and Jeffrey, Jones and Obradović, Aleksa",
year = "201",
abstract = "Two plant-tumorigenic strains KFB 330T and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A. nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD, glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, “A. fabrum” and A. pusense. This was further supported by average nucleotide identity values (<92%) between the whole genome sequences of strain KFB 330T and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8–77.87%) and 16:0 (6.82–8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain of A. arsenijevicii is KFB 330T (=CFBP 8308T = LMG 28674T).",
publisher = "Elsevier",
journal = "Systematic and Applied Microbiology",
title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum",
pages = "378-373",
number = "6",
volume = "38",
doi = "10.1016/j.syapm.2015.06.001"
}

Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jeffrey, J.,& Obradović, A.. (201). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology
Elsevier., 38(6), 373-378.
https://doi.org/10.1016/j.syapm.2015.06.001

Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jeffrey J, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 201;38(6):373-378.
doi:10.1016/j.syapm.2015.06.001 .

Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Anđelka, Ivanović, Milan, Zlatković, Nevena, Jeffrey, Jones, Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (201):373-378,
https://doi.org/10.1016/j.syapm.2015.06.001 . .