TY  - JOUR
AU  - Jankiev, Tamara
AU  - Berić, Tanja
AU  - Stević, Tatjana
AU  - Stanković, Slaviša
AU  - Bačić, Jasmina
AU  - Majstorović, Helena
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - https://enauka.gov.rs/handle/123456789/779167
UR  - https://intam.institut-tamis.rs/handle/123456789/218
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1190
AB  - The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed Pantoea, Sphingomonas, Hymenobacter, Massilia, and Pseudomonas as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were Metschnikowia, Filobasidium, Aureobasidium pullulans, Botrytis cinerea, and Taphrina. The traditional culturable approach revealed that the Pseudomonas genus with P. graminis, P. putida, and P. congelans was most prevalent. The most frequently cultivated fungal representatives belonged to the genus Fusarium with six identified species. A broad range of the antagonistic activity was detected for the Hannaella luteola and Metschnikowia pulcherrima yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. Fusarium sporotrichioides was the most susceptible fungal isolate. The autochthonous antagonistic yeasts H. luteola and M. pulcherrima might be powerful biological control agents of postharvest diseases caused by Fusarium spp. and common pathogens like Monilinia laxa, Botrytis cinerea, Alternaria tenuissima, and Cladosporium cladosporioides.
PB  - MDPI
T2  - Microorganisms
T1  - The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates
IS  - 7
SP  - 1282
VL  - 10
DO  - 10.3390/microorganisms10071282
ER  - 

@article{
author = "Jankiev, Tamara and Berić, Tanja and Stević, Tatjana and Stanković, Slaviša and Bačić, Jasmina and Majstorović, Helena and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed Pantoea, Sphingomonas, Hymenobacter, Massilia, and Pseudomonas as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were Metschnikowia, Filobasidium, Aureobasidium pullulans, Botrytis cinerea, and Taphrina. The traditional culturable approach revealed that the Pseudomonas genus with P. graminis, P. putida, and P. congelans was most prevalent. The most frequently cultivated fungal representatives belonged to the genus Fusarium with six identified species. A broad range of the antagonistic activity was detected for the Hannaella luteola and Metschnikowia pulcherrima yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. Fusarium sporotrichioides was the most susceptible fungal isolate. The autochthonous antagonistic yeasts H. luteola and M. pulcherrima might be powerful biological control agents of postharvest diseases caused by Fusarium spp. and common pathogens like Monilinia laxa, Botrytis cinerea, Alternaria tenuissima, and Cladosporium cladosporioides.",
publisher = "MDPI",
journal = "Microorganisms",
title = "The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates",
number = "7",
pages = "1282",
volume = "10",
doi = "10.3390/microorganisms10071282"
}

Jankiev, T., Berić, T., Stević, T., Stanković, S., Bačić, J., Majstorović, H., Fira, Đ.,& Dimkić, I.. (2022). The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates. in Microorganisms
MDPI., 10(7), 1282.
https://doi.org/10.3390/microorganisms10071282

Jankiev T, Berić T, Stević T, Stanković S, Bačić J, Majstorović H, Fira Đ, Dimkić I. The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates. in Microorganisms. 2022;10(7):1282.
doi:10.3390/microorganisms10071282 .

Jankiev, Tamara, Berić, Tanja, Stević, Tatjana, Stanković, Slaviša, Bačić, Jasmina, Majstorović, Helena, Fira, Đorđe, Dimkić, Ivica, "The Microbiome of the 'Williams' Pear Variety Grown in the Organic Orchard and Antifungal Activity by the Autochthonous Bacterial and Yeast Isolates" in Microorganisms, 10, no. 7 (2022):1282,
https://doi.org/10.3390/microorganisms10071282 . .

TY  - JOUR
AU  - Nikolić, I.
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Popović, Tatjana
AU  - Lozo, J.
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/577
AB  - Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.
PB  - Hoboken : Wiley
T2  - Journal of Applied Microbiology
T1  - Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains
EP  - 176
IS  - 1
SP  - 165
VL  - 126
DO  - 10.1111/jam.14070
ER  - 

@article{
author = "Nikolić, I. and Berić, Tanja and Dimkić, Ivica and Popović, Tatjana and Lozo, J. and Fira, Đorđe and Stanković, Slaviša",
year = "2019",
abstract = "Aim Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). Methods and Results The minimum inhibitory concentration (MIC) of CLEs and their combinations against the pathogen and potential interaction between the extracts were determined in vitro. The most effective antibacterial activity was achieved with the CLE from B. amyloliquefaciens SS-12.6, with an MIC value of 0 center dot 63 mg ml(-1). Interactions between CLE combinations were mostly indifferent. The biocontrol potential of CLEs, mixtures of CLEs, and cell culture of B. amyloliquefaciens SS-12.6 was tested on sugar beet plants inoculated with P. syringae pv. aptata P53. The best result in inhibiting the appearance of tissue necrosis (up to 92%) was achieved with B. amyloliquefaciens SS-12.6 cell culture. Conclusion This work demonstrated significant biocontrol potential of the CLE and cell culture of B. amyloliquefaciens SS-12.6 which successfully suppress leaf spot disease severity on sugar beet plants. Significance and Impact of the Study The findings of biocontrol of sugar beet emerging pathogen will contribute to growers in terms of alternative disease control management. This study represents first assessment of biological control of P. syringae pv. aptata.",
publisher = "Hoboken : Wiley",
journal = "Journal of Applied Microbiology",
title = "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains",
pages = "176-165",
number = "1",
volume = "126",
doi = "10.1111/jam.14070"
}

Nikolić, I., Berić, T., Dimkić, I., Popović, T., Lozo, J., Fira, Đ.,& Stanković, S.. (2019). Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology
Hoboken : Wiley., 126(1), 165-176.
https://doi.org/10.1111/jam.14070

Nikolić I, Berić T, Dimkić I, Popović T, Lozo J, Fira Đ, Stanković S. Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains. in Journal of Applied Microbiology. 2019;126(1):165-176.
doi:10.1111/jam.14070 .

Nikolić, I., Berić, Tanja, Dimkić, Ivica, Popović, Tatjana, Lozo, J., Fira, Đorđe, Stanković, Slaviša, "Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains" in Journal of Applied Microbiology, 126, no. 1 (2019):165-176,
https://doi.org/10.1111/jam.14070 . .

TY  - JOUR
AU  - Krivokapić, Marija
AU  - Gavrilović, Veljko
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Fira, Đorđe
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/545
AB  - The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
AB  - Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia
T1  - Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji
EP  - 184
IS  - 3-4
SP  - 175
VL  - 33
DO  - 10.2298/PIF1804175K
ER  - 

@article{
author = "Krivokapić, Marija and Gavrilović, Veljko and Ivanović, Milan and Kuzmanović, Nemanja and Fira, Đorđe and Obradović, Aleksa and Gašić, Katarina",
year = "2018",
abstract = "The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation., Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia, Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji",
pages = "184-175",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804175K"
}

Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, Đ., Obradović, A.,& Gašić, K.. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 175-184.
https://doi.org/10.2298/PIF1804175K

Krivokapić M, Gavrilović V, Ivanović M, Kuzmanović N, Fira Đ, Obradović A, Gašić K. Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina. 2018;33(3-4):175-184.
doi:10.2298/PIF1804175K .

Krivokapić, Marija, Gavrilović, Veljko, Ivanović, Milan, Kuzmanović, Nemanja, Fira, Đorđe, Obradović, Aleksa, Gašić, Katarina, "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):175-184,
https://doi.org/10.2298/PIF1804175K . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Janse, Jaap
AU  - Kojić, Milan
AU  - Stanković, Slaviša
AU  - Gavrilović, Veljko
AU  - Fira, Đorđe
PY  - 2015
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/396
AB  - The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques
EP  - 145
IS  - 1
SP  - 133
VL  - 141
DO  - 10.1007/s10658-014-0531-5
ER  - 

@article{
author = "Ivanović, Žarko and Popović, Tatjana and Janse, Jaap and Kojić, Milan and Stanković, Slaviša and Gavrilović, Veljko and Fira, Đorđe",
year = "2015",
abstract = "The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques",
pages = "145-133",
number = "1",
volume = "141",
doi = "10.1007/s10658-014-0531-5"
}

Ivanović, Ž., Popović, T., Janse, J., Kojić, M., Stanković, S., Gavrilović, V.,& Fira, Đ.. (2015). Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology
Springer, Dordrecht., 141(1), 133-145.
https://doi.org/10.1007/s10658-014-0531-5

Ivanović Ž, Popović T, Janse J, Kojić M, Stanković S, Gavrilović V, Fira Đ. Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology. 2015;141(1):133-145.
doi:10.1007/s10658-014-0531-5 .

Ivanović, Žarko, Popović, Tatjana, Janse, Jaap, Kojić, Milan, Stanković, Slaviša, Gavrilović, Veljko, Fira, Đorđe, "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques" in European Journal of Plant Pathology, 141, no. 1 (2015):133-145,
https://doi.org/10.1007/s10658-014-0531-5 . .

TY  - CHAP
AU  - Ivanović, Žarko
AU  - Gavrilovic, Veljko
AU  - Popović Milovanović, Tatjana
AU  - Zivkovic, Svetlana
AU  - Blagojević, Jovana
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1074
PB  - Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India
T2  - Technological Advancement for Vibrant Agriculture
T1  - Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates
EP  - 159
SP  - 153
VL  - 7
ER  - 

@inbook{
author = "Ivanović, Žarko and Gavrilovic, Veljko and Popović Milovanović, Tatjana and Zivkovic, Svetlana and Blagojević, Jovana and Stanković, Slaviša and Fira, Đorđe",
year = "2014",
publisher = "Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India",
journal = "Technological Advancement for Vibrant Agriculture",
booktitle = "Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates",
pages = "159-153",
volume = "7"
}

Ivanović, Ž., Gavrilovic, V., Popović Milovanović, T., Zivkovic, S., Blagojević, J., Stanković, S.,& Fira, Đ.. (2014). Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates. in Technological Advancement for Vibrant Agriculture
Dr. Amitava Rakshit, Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences Banaras Hindu University, India., 7, 153-159.

Ivanović Ž, Gavrilovic V, Popović Milovanović T, Zivkovic S, Blagojević J, Stanković S, Fira Đ. Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates. in Technological Advancement for Vibrant Agriculture. 2014;7:153-159..

Ivanović, Žarko, Gavrilovic, Veljko, Popović Milovanović, Tatjana, Zivkovic, Svetlana, Blagojević, Jovana, Stanković, Slaviša, Fira, Đorđe, "Antimicrobial Activity of Bacillus Spp. in the Biocontrol of Different Phytopathogenic Agrobacterium Isolates" in Technological Advancement for Vibrant Agriculture, 7 (2014):153-159.

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Živković, Svetlana
AU  - Stanković, Slaviša
AU  - Berić, Tanja
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/251
AB  - During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia.
AB  - Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.
PB  - Serbian Genetics Society
T2  - Genetika-Belgrade
T1  - Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia
T1  - Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji
EP  - 961
IS  - 3
SP  - 953
VL  - 45
DO  - 10.2298/GENSR1303953G
ER  - 

@article{
author = "Gavrilović, Veljko and Ivanović, Žarko and Popović, Tatjana and Živković, Svetlana and Stanković, Slaviša and Berić, Tanja and Fira, Đorđe",
year = "2013",
abstract = "During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia., Tokom nekoliko poslednjih godina u nekim regionima Srbije, značajnim za proizvodnju trešanja, uočeni su simptomi nekroze pupoljaka ovog voća. Sa margina nekrotičnog tkiva izolovani su sojevi gram negativnih, fluorescentnih, oksidativnih bakterija. Svi ispitivani sojevi su bili levan i HR pozitivni dok su negativni rezultati zabeleženi u oksidaza, pektinaza i dihidrolaza testovima (LOPAT +---+). Simptomi slični onima zabeleženim u prirodnim infekcijama dobijeni su nakon veštačke inokulacije lisnih ožiljaka i dormantnih jednogodišnjih sadnica trešnje. Ispitivani sojevi, kao i referentni soj Pseudomonas syringae pv. morsprunorum izazivaju površinsku nekrozu na veštačkim nezrelim plodovima trešnje, ali negativni rezultati su zabeleženi na nezrelim plodovima kruške i limuna kao i na lišću jorgovana i mahunama pasulja. Ispitivani sojevi izolovani sa nekrotičnih pupoljaka trešnje i šljive imali su identičan REP-PCR profil traka kao i referentni soj P. syringae pv. morsprunorum. Dobijeni rezultati ukazuju na to da je uzročnik nekroze pupoljaka trešnje bakterijska infekcija sojem P. syringae pv. morsprunorum.",
publisher = "Serbian Genetics Society",
journal = "Genetika-Belgrade",
title = "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia, Genetička karakterizacija patogenih flueroscentnih pseudomonada izolovanih iz nekrotičnih trešnji i šljiva u Srbiji",
pages = "961-953",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303953G"
}

Gavrilović, V., Ivanović, Ž., Popović, T., Živković, S., Stanković, S., Berić, T.,& Fira, Đ.. (2013). Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade
Serbian Genetics Society., 45(3), 953-961.
https://doi.org/10.2298/GENSR1303953G

Gavrilović V, Ivanović Ž, Popović T, Živković S, Stanković S, Berić T, Fira Đ. Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia. in Genetika-Belgrade. 2013;45(3):953-961.
doi:10.2298/GENSR1303953G .

Gavrilović, Veljko, Ivanović, Žarko, Popović, Tatjana, Živković, Svetlana, Stanković, Slaviša, Berić, Tanja, Fira, Đorđe, "Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia" in Genetika-Belgrade, 45, no. 3 (2013):953-961,
https://doi.org/10.2298/GENSR1303953G . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Živković, Svetlana
AU  - Berić, Tanja
AU  - Ivanović, Žarko
AU  - Gavrilović, Veljko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
PY  - 2013
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/291
AB  - Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Biological Control
T1  - Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi
EP  - 321
IS  - 3
SP  - 312
VL  - 65
DO  - 10.1016/j.biocontrol.2013.03.012
ER  - 

@article{
author = "Dimkić, Ivica and Živković, Svetlana and Berić, Tanja and Ivanović, Žarko and Gavrilović, Veljko and Stanković, Slaviša and Fira, Đorđe",
year = "2013",
abstract = "Two strains of Bacillus sp., SS-12.6 and SS-13.1, showed very strong antibacterial and antifungal activity against phytopathogens. The PCR analysis showed that both strains have the genes for biosynthesis of iturin, bacillomycin and surfactin. Kinetics of production of antimicrobial substances in these strains showed that synthesis started at the beginning of exponential phase of growth. Maximum of activity was slowly reached at the beginning of stationary growth phase and was maintained until the end of observed period. Ethyl acetate extracts of cell-free supernatants of both strains were particularly active against several postharvest fungal pathogens, in vitro and in vivo, in the experiment with apple fruits. Mass spectrometry analysis of ethyl acetate extract of the supernatant of strain SS-12.6 confirmed the presence of antimicrobial lipopeptide surfactin. (c) 2013 Elsevier Inc. All rights reserved.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Biological Control",
title = "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi",
pages = "321-312",
number = "3",
volume = "65",
doi = "10.1016/j.biocontrol.2013.03.012"
}

Dimkić, I., Živković, S., Berić, T., Ivanović, Ž., Gavrilović, V., Stanković, S.,& Fira, Đ.. (2013). Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control
Academic Press Inc Elsevier Science, San Diego., 65(3), 312-321.
https://doi.org/10.1016/j.biocontrol.2013.03.012

Dimkić I, Živković S, Berić T, Ivanović Ž, Gavrilović V, Stanković S, Fira Đ. Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi. in Biological Control. 2013;65(3):312-321.
doi:10.1016/j.biocontrol.2013.03.012 .

Dimkić, Ivica, Živković, Svetlana, Berić, Tanja, Ivanović, Žarko, Gavrilović, Veljko, Stanković, Slaviša, Fira, Đorđe, "Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi" in Biological Control, 65, no. 3 (2013):312-321,
https://doi.org/10.1016/j.biocontrol.2013.03.012 . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Stanković, Slaviša
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Kojić, Milan
AU  - Fira, Đorđe
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/208
AB  - Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia
EP  - 203
IS  - 1
SP  - 191
VL  - 134
DO  - 10.1007/s10658-012-9978-4
ER  - 

@article{
author = "Ivanović, Žarko and Stanković, Slaviša and Živković, Svetlana and Gavrilović, Veljko and Kojić, Milan and Fira, Đorđe",
year = "2012",
abstract = "Infection of fruit trees by Pseudomonas syringae is a potentially serious problem that may limit the establishment and sustained productivity of pome and stone fruit orchards in Serbia. To estimate possible diversity of Pseudomonas syringae fruit trees strains, we collected a set of strains in several areas of Serbia. The samples were taken from infected orchards with raspberry, plum, cherry, sour cherry, peach, pear and apple trees. Genetic diversity of P. syringae strains isolated from fruit trees was determined by using SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and REP-PCR. Molecular analysis showed that most of isolates had unique profiles, with the exception of isolates from plum and cherry that displayed profiles identical to each other and similar to P. syringae pv. morsprunorum. The study presented here clearly demonstrates the discriminative power of molecular techniques in enabling a detailed analysis of the genetic variations between strains of P. syringae from different pome and stone fruit hosts in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia",
pages = "203-191",
number = "1",
volume = "134",
doi = "10.1007/s10658-012-9978-4"
}

Ivanović, Ž., Stanković, S., Živković, S., Gavrilović, V., Kojić, M.,& Fira, Đ.. (2012). Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 134(1), 191-203.
https://doi.org/10.1007/s10658-012-9978-4

Ivanović Ž, Stanković S, Živković S, Gavrilović V, Kojić M, Fira Đ. Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia. in European Journal of Plant Pathology. 2012;134(1):191-203.
doi:10.1007/s10658-012-9978-4 .

Ivanović, Žarko, Stanković, Slaviša, Živković, Svetlana, Gavrilović, Veljko, Kojić, Milan, Fira, Đorđe, "Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia" in European Journal of Plant Pathology, 134, no. 1 (2012):191-203,
https://doi.org/10.1007/s10658-012-9978-4 . .