TY  - JOUR
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Menković, Jelena
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/715
AB  - During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.
PB  - APS Publications
T2  - PLANT DISEASE
T1  - Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia
IS  - 3
VL  - 107
DO  - 10.1094/PDIS-06-22-1425-RE
ER  - 

@article{
author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2023",
abstract = "During May 2016, severe blight symptoms were observed in several raspberry and blackberry fields in Serbia. In total, 22 strains were isolated: 16 from symptomatic raspberry shoots, 2 from asymptomatic raspberry leaves, and 4 from symptomatic blackberry shoots. Additionally, eight raspberry strains, isolated earlier from two similar outbreaks, were included in the study. Pathogenicity of the strains was confirmed on detached raspberry and blackberry shoots by reproducing the symptoms of natural infection. The strains were Gram-negative, fluorescent on King’s medium B, ice nucleation positive, and utilized glucose oxidatively. All strains were levan positive, oxidase negative, nonpectolytic, arginine dihydrolase negative, and induced hypersensitivity in tobacco leaves (LOPAT + −−− +, Pseudomonas group Ia). Furthermore, all strains liquefied gelatin and hydrolyzed aesculin but did not show tyrosinase activity or utilize tartrate (GATTa + + − −). Tentative identification using morphology, LOPAT, GATTa, and ice-nucleating ability tests suggested that isolated strains belong to Pseudomonas syringae. The syrB gene associated with syringomycin production was detected in all strains. DNA fingerprints with REP, ERIC, and BOX primers generated identical profiles for 29 strains, except for strain KBI 222, which showed a unique genomic fingerprint. In all, 9 of 10 selected strains exhibited identical sequences of four housekeeping genes: gyrB, rpoD, gapA, and gltA. Five nucleotide polymorphisms were found in strain KBI 222 at the rpoD gene locus only. In the phylogenetic tree based on a concatenated sequence of all four housekeeping genes, strains clustered within phylogroup 2 (i.e., genomospecies 1) of the P. syringae species complex, with pathotype strains of P. syringae pv. aceris and P. syringae pv. solidagae as their closest relatives. There was no correlation between genotype and geographic origin, particular outbreak, host, or cultivar.",
publisher = "APS Publications",
journal = "PLANT DISEASE",
title = "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia",
number = "3",
volume = "107",
doi = "10.1094/PDIS-06-22-1425-RE"
}

Ivanović, M., Prokić, A., Gašić, K., Menković, J., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2023). Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE
APS Publications., 107(3).
https://doi.org/10.1094/PDIS-06-22-1425-RE

Ivanović M, Prokić A, Gašić K, Menković J, Kuzmanović N, Zlatković N, Obradović A. Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia. in PLANT DISEASE. 2023;107(3).
doi:10.1094/PDIS-06-22-1425-RE .

Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Characterization of Pseudomonas syringae Strains Associated with Shoot Blight of Raspberry and Blackberry in Serbia" in PLANT DISEASE, 107, no. 3 (2023),
https://doi.org/10.1094/PDIS-06-22-1425-RE . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zivkovic, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/744
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
SP  - 235
VL  - 12
VL  - 2
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Zivkovic, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
pages = "235",
volume = "12, 2",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zivkovic, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12, 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zivkovic S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12:235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Zivkovic, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Lela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/725
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
IS  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Lela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
number = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, L.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12(803789).
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić L, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12(803789).
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Lela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12, no. 803789 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - CHAP
AU  - Choudhary, Manoj
AU  - Paret, Mathews
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Jeff, Jones
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/763
AB  - Crop yield loss due to bacterial plant pathogens need to be reduced to increase global food production demand. Currently available disease management strategies involving copper-based bactericides and antibiotics are losing efficacy due to development of resistance in bacteria. There is long familiar demand of environmentally friendly and sustainable strategies to control bacterial diseases. Bacteriophages are virus that kill target bacteria without affecting another microorganism and environment. Bacteriophage efficiency on phyllosphere is mainly affected by ultraviolet (UV) light. Use of combination of phage, mixture with phage carrier bacteria and optimizing time of application helps in persistence of bacteriophage. There are several bacteriophage products already available in the market to control destructive bacterial diseases. Unlike chemical based traditional control measure, bacteriophage mixture can be easily amended to reduce resistance development in bacteria. In this chapter, the authors discuss from phage isolation to interaction with bacteria and control mechanism of plant diseases.
PB  - Burleigh Dodds Science Publishing
T2  - In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.
T1  - Bacteriophages for plant disease control
EP  - 506
SP  - 473
DO  - 10.19103/AS.2021.0093.18
ER  - 

@inbook{
author = "Choudhary, Manoj and Paret, Mathews and Obradović, Aleksa and Gašić, Katarina and Jeff, Jones",
year = "2022",
abstract = "Crop yield loss due to bacterial plant pathogens need to be reduced to increase global food production demand. Currently available disease management strategies involving copper-based bactericides and antibiotics are losing efficacy due to development of resistance in bacteria. There is long familiar demand of environmentally friendly and sustainable strategies to control bacterial diseases. Bacteriophages are virus that kill target bacteria without affecting another microorganism and environment. Bacteriophage efficiency on phyllosphere is mainly affected by ultraviolet (UV) light. Use of combination of phage, mixture with phage carrier bacteria and optimizing time of application helps in persistence of bacteriophage. There are several bacteriophage products already available in the market to control destructive bacterial diseases. Unlike chemical based traditional control measure, bacteriophage mixture can be easily amended to reduce resistance development in bacteria. In this chapter, the authors discuss from phage isolation to interaction with bacteria and control mechanism of plant diseases.",
publisher = "Burleigh Dodds Science Publishing",
journal = "In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.",
booktitle = "Bacteriophages for plant disease control",
pages = "506-473",
doi = "10.19103/AS.2021.0093.18"
}

Choudhary, M., Paret, M., Obradović, A., Gašić, K.,& Jeff, J.. (2022). Bacteriophages for plant disease control. in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.
Burleigh Dodds Science Publishing., 473-506.
https://doi.org/10.19103/AS.2021.0093.18

Choudhary M, Paret M, Obradović A, Gašić K, Jeff J. Bacteriophages for plant disease control. in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506.. 2022;:473-506.
doi:10.19103/AS.2021.0093.18 .

Choudhary, Manoj, Paret, Mathews, Obradović, Aleksa, Gašić, Katarina, Jeff, Jones, "Bacteriophages for plant disease control" in In: Microbial bioprotectants for plant disease management (eds. Köhl, J and Ravensberg, W.), Burleigh Dodds Science Publishing, Sawston, Cambridge, UK, pp: 473-506. (2022):473-506,
https://doi.org/10.19103/AS.2021.0093.18 . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Menković, Jelena
AU  - Prokić, Anđelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://enauka.gov.rs/handle/123456789/776640
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1185
AB  - In Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro
EP  - 405
IS  - 2
SP  - 391
VL  - 128
DO  - 10.1007/s41348-020-00417-8
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Menković, Jelena and Prokić, Anđelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2021",
abstract = "In Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro",
pages = "405-391",
number = "2",
volume = "128",
doi = "10.1007/s41348-020-00417-8"
}

Popović Milovanović, T., Menković, J., Prokić, A., Zlatković, N.,& Obradović, A.. (2021). Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro. in Journal of Plant Diseases and Protection
Springer., 128(2), 391-405.
https://doi.org/10.1007/s41348-020-00417-8

Popović Milovanović T, Menković J, Prokić A, Zlatković N, Obradović A. Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro. in Journal of Plant Diseases and Protection. 2021;128(2):391-405.
doi:10.1007/s41348-020-00417-8 .

Popović Milovanović, Tatjana, Menković, Jelena, Prokić, Anđelka, Zlatković, Nevena, Obradović, Aleksa, "Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro" in Journal of Plant Diseases and Protection, 128, no. 2 (2021):391-405,
https://doi.org/10.1007/s41348-020-00417-8 . .

Koebnik, R., Gašić, K.,& Obradović, A.. (2021). Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe
Belgrade : University, Faculty of Agriculture..

Koebnik R, Gašić K, Obradović A. Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe. 2021;..

Koebnik, Ralf, Gašić, Katarina, Obradović, Aleksa, "Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe" in Integrating Science on Xanthomonadaceae for integrated plant disease management in Europe (2021).

Šević, M., Prokić, A., Gašić, K.,& Obradović, A.. (2021). Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Šević M, Prokić A, Gašić K, Obradović A. Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana. in Institut za zaštitu bilja i životnu sredinu. 2021;..

Šević, Milan, Prokić, Anđelka, Gašić, Katarina, Obradović, Aleksa, "Novi koncept održive strategije kontrole bakteriozne pegavosti paprike integracijom bioloških i hemijskih tretmana" in Institut za zaštitu bilja i životnu sredinu (2021).

TY  - CONF
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Gašić, Katarina
AU  - Grahovac, Mila
AU  - Duduk, Bojan
AU  - Milijašević-Marčić, Svetlana
AU  - Ignjatov, Maja
AU  - Šević, Milan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/812
AB  - Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
T1  - Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji
ER  - 

@conference{
author = "Obradović, Aleksa and Ivanović, Milan and Gašić, Katarina and Grahovac, Mila and Duduk, Bojan and Milijašević-Marčić, Svetlana and Ignjatov, Maja and Šević, Milan",
year = "2021",
abstract = "Istraživanja akademika Živojina Tešića iz 1934. godine, a zatim i prof. Dragoljuba Šutića
u prvoj deceniji posle drugog svetskog rata, na Poljoprivredno-šumarskom, kasnije
Poljoprivrednom fakultetu u Zemunu, mogu se smatrati početkom proučavanja fitopatogenih
bakterija (FPB) u Srbiji. Od tada do danas istraživači su prošli mukotrpan put tokom kojeg su
razvijali i širili znanja na polju ovog dela fitopatologije. Uvođenjem fitobakteriologije u
nastavu na Poljoprivrednim fakultetima u Novom Sadu i Beogradu, zahvaljujući entuzijazmu i
naučnoj dalekovidosti profesora Momčila Arsenijevića i Milana Panića, ova oblast istraživanja
dobila je na intenzitetu i značaju. Poslednjih decenija razvoj fitobakteriologije tekao je
dinamično i ubrzano, zahvaljujući sve bržem napretku nauke i tehnologije, razvoju sredstava
komunikacije, posvećenosti istraživača, naučnoj saradnji, studijskim boravcima u
međunarodnim obrazovnim i naučnim institucijama, novim metodama izučavanja i
olakšanom pristupu informacijama. U Srbiji, najviše pažnje je posvećeno proučavanju FPB, a
kasnije i fitoplazmi, poreklom iz ekonomski značajnih biljnih vrsta voćaka, vinove loze,
povrtarskih, ratarskih i ukrasnih biljaka. Zahvaljujući interesovanju i entuzijazmu nevelikog
broja istraživača, usvajane su najsavremenije metode istraživanja ovih patogena.
Fitobakteriološka istraživanja su među prvima uvela u primenu molekularne metode u
domaćoj fitopatologiji. Primena PCR metode omogućila je razvoj nove epohe istraživanja
prokariota u Srbiji i upotrebu tehnika kao što su sekvenciranje genoma, analiza specifičnih ili
konstitutivnih gena, kvantitativni ili Real-time PCR, a to su metode koje se danas rutinski
koriste u istrživanjima prokariota u Srbiji. U novijem periodu razvijena su istraživanja u
oblasti detekcije, identifikacije i karakterizacije fitoplazmi u Srbiji, prateći ili predvodeći
svetska saznanja u ovoj oblasti. Primena savremenih metoda istraživanja omogućila je
izučavanje genetske strukture populacija fitopatogenih prokariota, detekciju novih vrsta u
našoj zemlji, stvaranje markera za precizniju identifikaciju ili proširivanje znanja iz oblasti
epidemiologije fitopatogenih prokariota. Osim toga, stečena saznanja se koriste u razvoju
inovativnih mogućnosti zaštite od bakterioza biološkim i konvencionalnim metodama. Decenijama sticano i unapređivano znanje neprekidno se prenosi generacijama koje dolaze,
kroz učešće fitobakteriologa u nastavnom procesu i kroz mentorski rad u visokoškolskim i
naučnim ustanovama, stvarajući tako osnov za dalji razvoj i napredak fitobakteriologije u
Srbiji.",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19",
title = "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji"
}

Obradović, A., Ivanović, M., Gašić, K., Grahovac, M., Duduk, B., Milijašević-Marčić, S., Ignjatov, M.,& Šević, M.. (2021). Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19
Društvo za zaštitu bilja Srbije..

Obradović A, Ivanović M, Gašić K, Grahovac M, Duduk B, Milijašević-Marčić S, Ignjatov M, Šević M. Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19. 2021;..

Obradović, Aleksa, Ivanović, Milan, Gašić, Katarina, Grahovac, Mila, Duduk, Bojan, Milijašević-Marčić, Svetlana, Ignjatov, Maja, Šević, Milan, "Osvrt na proučavanje fitopatogenih bakterija i fitoplazmi u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. 18-19 (2021).

TY  - JOUR
AU  - Stefani, Emilio
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
AU  - Altin, Irem
AU  - Nagy, Ildikó K.
AU  - Kovács, Tamás
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5851
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/610
AB  - Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.
PB  - MDPI
T2  - Microorganisms
T1  - Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future
IS  - 5
SP  - 1056
VL  - 9
DO  - 10.3390/microorganisms9051056
ER  - 

@article{
author = "Stefani, Emilio and Obradović, Aleksa and Gašić, Katarina and Altin, Irem and Nagy, Ildikó K. and Kovács, Tamás",
year = "2021",
abstract = "Xanthomonads, members of the family Xanthomonadaceae, are economically important plant pathogenic bacteria responsible for infections of over 400 plant species. Bacteriophage-based biopesticides can provide an environmentally friendly, effective solution to control these bacteria. Bacteriophage-based biocontrol has important advantages over chemical pesticides, and treatment with these biopesticides is a minor intervention into the microflora. However, bacteriophages’ agricultural application has limitations rooted in these viruses’ biological properties as active sub-stances. These disadvantageous features, together with the complicated registration process of bacteriophage-based biopesticides, means that there are few products available on the market. This review summarizes our knowledge of the Xanthomonas-host plant and bacteriophage-host bacterium interaction’s possible influence on bacteriophage-based biocontrol strategies and provides examples of greenhouse and field trials and products readily available in the EU and the USA. It also details the most important advantages and limitations of the agricultural application of bacteriophages. This paper also investigates the legal background and industrial property right issues of bacteriophage-based biopesticides. When appropriately applied, bacteriophages can provide a promising tool against xanthomonads, a possibility that is untapped. Information presented in this review aims to explore the potential of bacteriophage-based biopesticides in the control of xanthomonads in the future.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future",
number = "5",
pages = "1056",
volume = "9",
doi = "10.3390/microorganisms9051056"
}

Stefani, E., Obradović, A., Gašić, K., Altin, I., Nagy, I. K.,& Kovács, T.. (2021). Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms
MDPI., 9(5), 1056.
https://doi.org/10.3390/microorganisms9051056

Stefani E, Obradović A, Gašić K, Altin I, Nagy IK, Kovács T. Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future. in Microorganisms. 2021;9(5):1056.
doi:10.3390/microorganisms9051056 .

Stefani, Emilio, Obradović, Aleksa, Gašić, Katarina, Altin, Irem, Nagy, Ildikó K., Kovács, Tamás, "Bacteriophage-mediated control of phytopathogenic xanthomonads: A promising green solution for the future" in Microorganisms, 9, no. 5 (2021):1056,
https://doi.org/10.3390/microorganisms9051056 . .

TY  - CHAP
AU  - Obradović, Aleksa
AU  - Jones, Jeffrey B
AU  - Botond, Balogh
AU  - Gašić, Katarina
PY  - 2020-06
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/764
AB  - Lytic bacteriophages (phages) have the potential for controlling susceptible bacteria in the rhizosphere or phyllosphere. The success of phage application in plant disease control requires that high populations of both phage and bacterium exist in order to initiate a chain reaction of bacterial lysis. Physical factors in natural environments such as the presence of biofilms that trap phages, low soil pH which inactivates phages, low rates of diffusion of phages in soil that prevent contact with target bacteria, and inactivation of phages upon exposure to ultraviolet (UV) light and desiccation, all impact successful use of phages in control of plant pathogenic bacteria. Other considerations relate to the bacterial strains which exist in nature. The bacterial species may have a low or high degree of variation in sensitivity to phages. Therefore, phage selection for field use requires careful monitoring of the targeted bacterial strains in the field due to the potential for strain variation and the likelihood for development of resistance to the deployed phages. Application timing has also been shown to be an important factor in improving the efficacy of phages. For instance, UV light is deleterious to phages and upon exposure phage populations plummet; therefore, evening applications of phages result in persistence on leaf surfaces for longer periods of time and may result in improved disease control. Extending the period of time that phages persist in the phyllosphere has been a major hurdle. Formulations have been identified which improve the persistence of phages on leaf surfaces; however, there is a need to identify superior formulations that extend the life on leaf surfaces from hours to days. Another strategy for maintaining high populations of phages has been to use non-pathogenic bacterial strains that are sensitive to the phage(s) or a closely related organism that does not cause disease on the plant host. Finally, phages may have value as part of an integrated management strategy.
PB  - Caister Academic Press
T2  - In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.
T1  - Considerations for Using Bacteriophages in Plant Pathosystems
EP  - 282
SP  - 257
DO  - 10.21775/9781913652517.07
ER  - 

@inbook{
author = "Obradović, Aleksa and Jones, Jeffrey B and Botond, Balogh and Gašić, Katarina",
year = "2020-06",
abstract = "Lytic bacteriophages (phages) have the potential for controlling susceptible bacteria in the rhizosphere or phyllosphere. The success of phage application in plant disease control requires that high populations of both phage and bacterium exist in order to initiate a chain reaction of bacterial lysis. Physical factors in natural environments such as the presence of biofilms that trap phages, low soil pH which inactivates phages, low rates of diffusion of phages in soil that prevent contact with target bacteria, and inactivation of phages upon exposure to ultraviolet (UV) light and desiccation, all impact successful use of phages in control of plant pathogenic bacteria. Other considerations relate to the bacterial strains which exist in nature. The bacterial species may have a low or high degree of variation in sensitivity to phages. Therefore, phage selection for field use requires careful monitoring of the targeted bacterial strains in the field due to the potential for strain variation and the likelihood for development of resistance to the deployed phages. Application timing has also been shown to be an important factor in improving the efficacy of phages. For instance, UV light is deleterious to phages and upon exposure phage populations plummet; therefore, evening applications of phages result in persistence on leaf surfaces for longer periods of time and may result in improved disease control. Extending the period of time that phages persist in the phyllosphere has been a major hurdle. Formulations have been identified which improve the persistence of phages on leaf surfaces; however, there is a need to identify superior formulations that extend the life on leaf surfaces from hours to days. Another strategy for maintaining high populations of phages has been to use non-pathogenic bacterial strains that are sensitive to the phage(s) or a closely related organism that does not cause disease on the plant host. Finally, phages may have value as part of an integrated management strategy.",
publisher = "Caister Academic Press",
journal = "In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.",
booktitle = "Considerations for Using Bacteriophages in Plant Pathosystems",
pages = "282-257",
doi = "10.21775/9781913652517.07"
}

Obradović, A., Jones, J. B., Botond, B.,& Gašić, K.. (2020-06). Considerations for Using Bacteriophages in Plant Pathosystems. in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.
Caister Academic Press., 257-282.
https://doi.org/10.21775/9781913652517.07

Obradović A, Jones JB, Botond B, Gašić K. Considerations for Using Bacteriophages in Plant Pathosystems. in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282.. 2020;:257-282.
doi:10.21775/9781913652517.07 .

Obradović, Aleksa, Jones, Jeffrey B, Botond, Balogh, Gašić, Katarina, "Considerations for Using Bacteriophages in Plant Pathosystems" in In: Bacterial Viruses: Exploitation for Biocontrol and Therapeutics (eds. Coffey, A. and Buttimer, C.), Caister Academic Press, Poole, UK, pp: 257-282. (2020-06):257-282,
https://doi.org/10.21775/9781913652517.07 . .

Popović Milovanović, T., Menković, J., Prokić, A.,& Obradović, A.. (2020). First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease
American Phytopathological Society., 104, 560-561.
https://doi.org/10.1094/PDIS-07-19-1422-PDN

Popović Milovanović T, Menković J, Prokić A, Obradović A. First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro. in Plant Disease. 2020;104:560-561.
doi:10.1094/PDIS-07-19-1422-PDN .

Popović Milovanović, Tatjana, Menković, J., Prokić, Anđelka, Obradović, Aleksa, "First Report of Xanthomonas arboricola pv. pruni Causing Leaf Spot and Twig Necrosis on Peach (Prunus persica) in Montenegro" in Plant Disease, 104 (2020):560-561,
https://doi.org/10.1094/PDIS-07-19-1422-PDN . .

TY  - JOUR
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Gašić, K.
AU  - Pavlović, Z.
AU  - Obradović, Aleksa
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/592
AB  - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
EP  - 691
IS  - 3
SP  - 685
VL  - 157
DO  - 10.1007/s10658-020-02019-4
ER  - 

@article{
author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa",
year = "2020",
abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia",
pages = "691-685",
number = "3",
volume = "157",
doi = "10.1007/s10658-020-02019-4"
}

Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology
Springer, Dordrecht., 157(3), 685-691.
https://doi.org/10.1007/s10658-020-02019-4

Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691.
doi:10.1007/s10658-020-02019-4 .

Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691,
https://doi.org/10.1007/s10658-020-02019-4 . .

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

Gašić, K., Šević, M.,& Obradović, A.. (2019). Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Gašić K, Šević M, Obradović A. Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti. in Institut za zaštitu bilja i životnu sredinu. 2019;..

Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Bakteriofag KF1 - novi biološki agens za zaštitu paprike od bakteriozne pegavosti" in Institut za zaštitu bilja i životnu sredinu (2019).

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Prokić, Anđelka
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1143
AB  - Watermelon (Citrullus lanatus) and squash (Cucurbita pepo) are traditionally grown in northern Serbia, in the province of Vojvodina. Most of the cucurbit cultivars grown in Serbia originate from imported seed. In July 2013, round, water-soaked lesions were observed on the fruits on sporadic squash plants grown in two fields of the breeder variety trials in Bački Petrovac. Affected tissue was light brown, slightly sunken, soft, and macerated. From symptomatic plants (cultivar GL Maxima), showing 30% symptom intensity, a uniform population of bacterial colonies was isolated, and one strain was chosen for the collection. In August of the following season, infection of the vine was observed on watermelon plants (cultivar unknown) grown in a commercial field in Čelarevo. Soft rot brownish lesions developed on infected stems, resulting in collapse and wilting of entire vines. The number of plants affected with symptoms was less than 10%. From the colonies isolated from the diseased tissue, two bacterial strains were purified and stored in the collection. Being pectolytic and isolated from cucurbits, the squash strain and two watermelon strains were subjected to further characterization. The strains developed white-gray, nonmucoid, round colonies with irregular margins on nutrient agar medium. They were gram and oxidase negative, tobacco HR and catalase positive, facultative anaerobes, nonfluorescent, caused soft rot on potato tuber slices, grew at 37°C, and reduced nitrate. They neither utilized arginine nor produced acid from sorbitol and inositol, showing characteristics of Pectobacterium species (De Boer and Kelman 2001). The PCR assay was carried out using primers BR1f/L1r (Duarte et al. 2004), specific for Pectobacterium carotovorum subsp. brasiliense (Pcb). A specific fragment of 322 bp was amplified for all three strains tested. The 16S rDNA sequence analysis (GenBank nos. MH031784, MH031785, and MH031786) showed 99% identity to the sequences of Pcb isolated from cucumber (CP020350), pepper (KX377597), and cabbage (KY021040) in China and potato in Japan (LC146476). The recA (MK911735, MK911736, and MK911737) and gapA (MK911738, MK911739, and MK911740) sequence analysis of three strains showed the highest nucleotide identity (100 to 98.35% and 99.42 to 98.08%, respectively) with Pcb previously deposited in NCBI GenBank database. Pathogenicity of the strains was tested on 2-week-old watermelon (cv. Rosa) and melon (cv. Sezam) seedlings, grown in a commercial potting mix in a greenhouse, as well as on mature zucchini fruits. Three seedlings per strain were inoculated by spraying with bacterial suspension (approx. 1 × 108 CFU/ml) using a handheld sprayer. Inoculated plants were incubated under plastic bags for 24 h and afterward maintained in a greenhouse at 28 ± 2°C. Two days after inoculation, water-soaked lesions developed on cotyledons of the watermelon and melon plants. No symptoms were observed on control plants sprayed with water. Three zucchini fruits were inoculated by pricking with a syringe and hypodermic needle and leaving a droplet of bacterial suspension (approx. 1 × 107 CFU/ml) at the point of inoculation. Inoculated fruits were placed on wet filter paper in a sealed plastic container at 28 ± 2°C. Treatment with sterile distilled water was used as a negative control. Discoloration of the tissue and soft rot spreading from the inoculation point developed within 48 h from inoculation. No symptoms developed on the control fruits. To complete Koch’s postulates, bacteria were reisolated from the inoculated plant and fruit tissue and were identified as Pcb by PCR assay (Duarte et al. 2004). This is the first report of Pcb causing soft rot of squash and watermelon in Serbia. The origin of the pathogen remained unknown. It might have been present in Serbia for some time but misidentified in previous studies that could not discriminate this subspecies or recently introduced by infected seed.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia
IS  - 10
SP  - 2667
VL  - 103
DO  - 10.1094/PDIS-12-18-2213-PDN
ER  - 

@article{
author = "Zlatković, Nevena and Prokić, Anđelka and Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Obradović, Aleksa",
year = "2019",
abstract = "Watermelon (Citrullus lanatus) and squash (Cucurbita pepo) are traditionally grown in northern Serbia, in the province of Vojvodina. Most of the cucurbit cultivars grown in Serbia originate from imported seed. In July 2013, round, water-soaked lesions were observed on the fruits on sporadic squash plants grown in two fields of the breeder variety trials in Bački Petrovac. Affected tissue was light brown, slightly sunken, soft, and macerated. From symptomatic plants (cultivar GL Maxima), showing 30% symptom intensity, a uniform population of bacterial colonies was isolated, and one strain was chosen for the collection. In August of the following season, infection of the vine was observed on watermelon plants (cultivar unknown) grown in a commercial field in Čelarevo. Soft rot brownish lesions developed on infected stems, resulting in collapse and wilting of entire vines. The number of plants affected with symptoms was less than 10%. From the colonies isolated from the diseased tissue, two bacterial strains were purified and stored in the collection. Being pectolytic and isolated from cucurbits, the squash strain and two watermelon strains were subjected to further characterization. The strains developed white-gray, nonmucoid, round colonies with irregular margins on nutrient agar medium. They were gram and oxidase negative, tobacco HR and catalase positive, facultative anaerobes, nonfluorescent, caused soft rot on potato tuber slices, grew at 37°C, and reduced nitrate. They neither utilized arginine nor produced acid from sorbitol and inositol, showing characteristics of Pectobacterium species (De Boer and Kelman 2001). The PCR assay was carried out using primers BR1f/L1r (Duarte et al. 2004), specific for Pectobacterium carotovorum subsp. brasiliense (Pcb). A specific fragment of 322 bp was amplified for all three strains tested. The 16S rDNA sequence analysis (GenBank nos. MH031784, MH031785, and MH031786) showed 99% identity to the sequences of Pcb isolated from cucumber (CP020350), pepper (KX377597), and cabbage (KY021040) in China and potato in Japan (LC146476). The recA (MK911735, MK911736, and MK911737) and gapA (MK911738, MK911739, and MK911740) sequence analysis of three strains showed the highest nucleotide identity (100 to 98.35% and 99.42 to 98.08%, respectively) with Pcb previously deposited in NCBI GenBank database. Pathogenicity of the strains was tested on 2-week-old watermelon (cv. Rosa) and melon (cv. Sezam) seedlings, grown in a commercial potting mix in a greenhouse, as well as on mature zucchini fruits. Three seedlings per strain were inoculated by spraying with bacterial suspension (approx. 1 × 108 CFU/ml) using a handheld sprayer. Inoculated plants were incubated under plastic bags for 24 h and afterward maintained in a greenhouse at 28 ± 2°C. Two days after inoculation, water-soaked lesions developed on cotyledons of the watermelon and melon plants. No symptoms were observed on control plants sprayed with water. Three zucchini fruits were inoculated by pricking with a syringe and hypodermic needle and leaving a droplet of bacterial suspension (approx. 1 × 107 CFU/ml) at the point of inoculation. Inoculated fruits were placed on wet filter paper in a sealed plastic container at 28 ± 2°C. Treatment with sterile distilled water was used as a negative control. Discoloration of the tissue and soft rot spreading from the inoculation point developed within 48 h from inoculation. No symptoms developed on the control fruits. To complete Koch’s postulates, bacteria were reisolated from the inoculated plant and fruit tissue and were identified as Pcb by PCR assay (Duarte et al. 2004). This is the first report of Pcb causing soft rot of squash and watermelon in Serbia. The origin of the pathogen remained unknown. It might have been present in Serbia for some time but misidentified in previous studies that could not discriminate this subspecies or recently introduced by infected seed.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia",
number = "10",
pages = "2667",
volume = "103",
doi = "10.1094/PDIS-12-18-2213-PDN"
}

Zlatković, N., Prokić, A., Gašić, K., Kuzmanović, N., Ivanović, M.,& Obradović, A.. (2019). First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease
American Phytopathological Society., 103(10), 2667.
https://doi.org/10.1094/PDIS-12-18-2213-PDN

Zlatković N, Prokić A, Gašić K, Kuzmanović N, Ivanović M, Obradović A. First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia. in Plant Disease. 2019;103(10):2667.
doi:10.1094/PDIS-12-18-2213-PDN .

Zlatković, Nevena, Prokić, Anđelka, Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Obradović, Aleksa, "First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia" in Plant Disease, 103, no. 10 (2019):2667,
https://doi.org/10.1094/PDIS-12-18-2213-PDN . .

TY  - JOUR
AU  - Newberry, Eric
AU  - Ebrahim, Mohamed
AU  - Timilsina, Sujan
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
AU  - Bull, Carolee
AU  - Goss, Erica
AU  - Huguet Tapia, Jose
AU  - Paret, Mathews
AU  - Jones, Jeffrey
AU  - Potnis, Neha
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/960
AB  - Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash
EP  - 18
SP  - 1
VL  - 10
DO  - 10.3389/fmicb.2019.00270
ER  - 

@article{
author = "Newberry, Eric and Ebrahim, Mohamed and Timilsina, Sujan and Zlatković, Nevena and Obradović, Aleksa and Bull, Carolee and Goss, Erica and Huguet Tapia, Jose and Paret, Mathews and Jones, Jeffrey and Potnis, Neha",
year = "2019",
abstract = "Pseudomonas syringae sensu strict , (phylogroup 2; referred to as P. syringae) consists of an environmentally ubiquitous bacterial population associated with diseases of numerous plant species. Recent studies using multilocus sequence analysis have indicated the clonal expansion of several P. syringae lineages, located in phylogroups 2a and 2b, in association with outbreaks of bacterial spot disease of watermelon, cantaloupe, and squash in the United States. To investigate the evolutionary processes that led to the emergence of these epidemic lineages, we sequenced the genomes of six P. syringae strains that were isolated from cucurbits grown in the United States, Europe, and China over a period of more than a decade, as well as eight strains that were isolated from watermelon and squash grown in six different Florida counties during the 2013 and 2014 seasons. These data were subjected to comparative analyses along with 42 previously sequenced genomes of P. syringae stains collected from diverse plant species and environments available from GenBank. Maximum likelihood reconstruction of the P. syringae core genome revealed the presence of a hybrid phylogenetic group, comprised of cucurbit strains collected in Florida, Italy, Serbia, and France, which emerged through genome-wide homologous recombination between phylogroups 2a and 2b. Functional analysis of the recombinant core genome showed that pathways involved in the ATP-dependent transport and metabolism of amino acids, bacterial motility, and secretion systems were enriched for recombination. A survey of described virulence factors indicated the convergent acquisition of several accessory type 3 secreted effectors (T3SEs) among phylogenetically distinct lineages through integrative and conjugative element and plasmid loci. Finally, pathogenicity assays on watermelon and squash showed qualitative differences in virulence between strains of the same clonal lineage, which correlated with T3SEs acquired through various mechanisms of horizontal gene transfer (HGT). This study provides novel insights into the interplay of homologous recombination and HGT toward pathogen emergence and highlights the dynamic nature of P. syringae sensu lato genomes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash",
pages = "18-1",
volume = "10",
doi = "10.3389/fmicb.2019.00270"
}

Newberry, E., Ebrahim, M., Timilsina, S., Zlatković, N., Obradović, A., Bull, C., Goss, E., Huguet Tapia, J., Paret, M., Jones, J.,& Potnis, N.. (2019). Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 10, 1-18.
https://doi.org/10.3389/fmicb.2019.00270

Newberry E, Ebrahim M, Timilsina S, Zlatković N, Obradović A, Bull C, Goss E, Huguet Tapia J, Paret M, Jones J, Potnis N. Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash. in Frontiers in Microbiology. 2019;10:1-18.
doi:10.3389/fmicb.2019.00270 .

Newberry, Eric, Ebrahim, Mohamed, Timilsina, Sujan, Zlatković, Nevena, Obradović, Aleksa, Bull, Carolee, Goss, Erica, Huguet Tapia, Jose, Paret, Mathews, Jones, Jeffrey, Potnis, Neha, "Inference of Convergent Gene Acquisition Among Pseudomonas syringae Strains Isolated From Watermelon, Cantaloupe, and Squash" in Frontiers in Microbiology, 10 (2019):1-18,
https://doi.org/10.3389/fmicb.2019.00270 . .

TY  - JOUR
AU  - Šević, Milan
AU  - Gašić, Katarina
AU  - Ignjatov, Maja
AU  - Mijatović, Mirjana
AU  - Prokić, Anđelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/576
AB  - Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.
PB  - Elsevier
T2  - Crop Protection
T1  - Integration of biological and conventional treatments in control of pepper bacterial spot
EP  - 51
SP  - 46
VL  - 119
DO  - 10.1016/j.cropro.2019.01.006
ER  - 

@article{
author = "Šević, Milan and Gašić, Katarina and Ignjatov, Maja and Mijatović, Mirjana and Prokić, Anđelka and Obradović, Aleksa",
year = "2019",
abstract = "Bacterial spot caused by Xanthomonas euvesicatoria is one of the most devastating pepper diseases in Serbia. Questionable seed quality, climatic conditions, and frequent irrigation during summer favour the disease occurrence and spread. The available management practices do not provide adequate disease control. Therefore, development of alternative and more sustainable disease management strategies is needed. Integration of classical and biological treatments could be an effective, environmentally safe option for reducing pepper bacterial spot severity. In order to develop an efficient integrated disease management program, we studied efficacy of biocontrol agents (bacteriophage strain KФ1 and two strains of Bacillus subtilis AAac and QST 713), systemic acquired resistance (SAR) inducer (acibenzolar-S-methyl - ASM), a commercial microbial fertilizer (Slavol), copper based compounds (copper hydroxide and copper oxychloride) in combination with or without mancozeb, and antibiotics (streptomycin sulphate and kasugamycin). They were applied as single treatments in two separate field experiments. Based on the single treatment efficacy, various combinations of the treatments were chosen for further testing in three separate field experiments. Additionally, we evaluated potential negative effect of ASM on pepper growth and yield in the growth chamber experiment. All the tested single treatments significantly reduced disease severity compared to the inoculated control (IC), except microbiological fertilizer and the antagonistic strain AAac. Integration of copper hydroxide, ASM and bacteriophages was the most efficient treatment, reducing the disease intensity by 96–98%. The results indicated that this combination may be an adequate alternative program for control of pepper bacterial spot.",
publisher = "Elsevier",
journal = "Crop Protection",
title = "Integration of biological and conventional treatments in control of pepper bacterial spot",
pages = "51-46",
volume = "119",
doi = "10.1016/j.cropro.2019.01.006"
}

Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A.,& Obradović, A.. (2019). Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection
Elsevier., 119, 46-51.
https://doi.org/10.1016/j.cropro.2019.01.006

Šević M, Gašić K, Ignjatov M, Mijatović M, Prokić A, Obradović A. Integration of biological and conventional treatments in control of pepper bacterial spot. in Crop Protection. 2019;119:46-51.
doi:10.1016/j.cropro.2019.01.006 .

Šević, Milan, Gašić, Katarina, Ignjatov, Maja, Mijatović, Mirjana, Prokić, Anđelka, Obradović, Aleksa, "Integration of biological and conventional treatments in control of pepper bacterial spot" in Crop Protection, 119 (2019):46-51,
https://doi.org/10.1016/j.cropro.2019.01.006 . .

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Obradović, Aleksa
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/597
AB  - Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species.
AB  - Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Bacterial diseases of cucurbits
T1  - Bakterioze tikava
EP  - 445
IS  - 6
SP  - 431
VL  - 47
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Andjelka and Obradović, Aleksa",
year = "2019",
abstract = "Production of cucurbits has a long tradition worldwide. Cucumber, watermelon and melon production is very important for many producers in some parts of Serbia. These crops are especially susceptible to phytopathogenic bacteria which can cause substantial losses in years with favorable weather conditions. In this paper major bacterial diseases of cucurbits are described, such as bacterial fruit blotch caused by Acidovorax citrulli, angular leaf spot caused by Pseudomonas syringae pv. lachrymans, bacterial spot caused by Xanthomonas campestris, bacterial wilt caused by Erwinia tracheiphila, cucurbit yellow wine disease caused by Serratia marcescens, bacterial leaf spot, caused by Pseudomonas syringae pv. syringae, inducing bacterial soft rot caused by Pectobacterium species., Biljke familije Cucurbitaceae imaju dugu tradiciju proizvodnje širom sveta. U pojedinim krajevima Srbije, naročito severnim, proizvodnja krastavca, lubenice i dinje predstavlja primarno zanimanje mnogih poljoprivrednih gazdinstava. Pojava bakterioza u godinama sa uslovima koji pogoduju nastanku infekcije, može značajno ugroziti proizvodnju ovih biljaka. U ovom radu predstavljena su najznačajnija bakteriozna oboljenja biljaka familije Cucurbitaceae, među kojima su: bakteriozna mrljavost plodova lubenice, čiji je prouzrokovač Acidovorax citrulli, uglasta pegavost lišća krastavca, prouzrokovana bakterijom Pseudomonas syringae pv. lachrymans; bakteriozna uvelost krastavca prouzrokovana vrstom Erwinia tracheiphila; bakteriozna pegavost koju prouzrokuje Xanthomonas cucurbitae; potom žutilo vreža, čiji je prouzrokovač Serratia marcescens; zatim bakteriozna lisna pegavost prouzrokovana Pseudomonas syringae pv. syringae; kao i bakteriozna vlažna trulež prouzrokovana vrstama roda Pectobacterium.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Bacterial diseases of cucurbits, Bakterioze tikava",
pages = "445-431",
number = "6",
volume = "47"
}

Zlatković, N., Gašić, K., Prokić, A.,& Obradović, A.. (2019). Bacterial diseases of cucurbits. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 47(6), 431-445.

Zlatković N, Gašić K, Prokić A, Obradović A. Bacterial diseases of cucurbits. in Biljni lekar. 2019;47(6):431-445..

Zlatković, Nevena, Gašić, Katarina, Prokić, Andjelka, Obradović, Aleksa, "Bacterial diseases of cucurbits" in Biljni lekar, 47, no. 6 (2019):431-445.

TY  - JOUR
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Gašić, Katarina
AU  - Prokić, Andjelka
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5009
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/601
AB  - Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material
EP  - 1052
IS  - 3
SP  - 1039
VL  - 51
DO  - 10.2298/GENSR1903039I
ER  - 

@article{
author = "Ivanović, Milan and Kuzmanović, Nemanja and Gašić, Katarina and Prokić, Andjelka and Zlatković, Nevena and Obradović, Aleksa",
year = "2019",
abstract = "Three PCR methods, referred in this study as,conventional" "nested" and,chromosomal" PCR and suggested for routine detection of Erwinia amylovora in pure culture and plant material, were evaluated according to their specificity and sensitivity. Specificity of PCR methods was analyzed by using 42 strains of E. amylovora, originating from different locations and plant species, with diverse PFGE profiles, representing distant populations of the pathogen. Sensitivity of PCR protocols in pure culture was studied by using nine different concentrations of E. amylovora in sterile ultrapure water as a template in PCR reactions. In order to study inhibitory effect of plant DNA and other inhibitors on sensitivity of the three PCR methods bacterial dilutions were mixed with plant macerate of pear, apple and quince prior to the PCR reaction. In specificity assays, tested PCR protocols were able to detect all E. amylovora strains regardless of the host of the strain, its origin or PFGE group, indicating primer specificity. On the other hand, sensitivity among tested methods varied, depending on bacterial concentration and selected plant material used in the PCR. When working with pure cultures nested PCR showed the greatest sensitivity by detecting 1.9 bacterial cells per PCR reaction, followed by detection limit of 9.5 cells per PCR reaction with conventional PCR and 1.9.105 cells/PCR reaction with chromosomal PCR. In spiked samples plant inhibitors either did not affect or they decreased the sensitivity of the PCR reaction, depending on the protocol and/or type of plant macerate. In our experiments, inhibitors from pear and quince macerates did not affect sensitivity of nested PCR, while apple macerate reduced its sensitivity by a factor of 10. Conventional PCR protocol was able to detect 95 cells/PCR reaction in pear and apple macerate, but only 9.5.103 cells/PCR in quince macerate. Greatest decrease in sensitivity of the PCR method was observed in spiked samples with chromosomal PCR since bacterial DNA was not detected in each of the spiked samples. Our research shows that all three PCR protocols are specific for detection of E. amylovora, but nested PCR proved to be most sensitive when working with pure cultures and plant material.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material",
pages = "1052-1039",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903039I"
}

Ivanović, M., Kuzmanović, N., Gašić, K., Prokić, A., Zlatković, N.,& Obradović, A.. (2019). Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika
Društvo genetičara Srbije, Beograd., 51(3), 1039-1052.
https://doi.org/10.2298/GENSR1903039I

Ivanović M, Kuzmanović N, Gašić K, Prokić A, Zlatković N, Obradović A. Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material. in Genetika. 2019;51(3):1039-1052.
doi:10.2298/GENSR1903039I .

Ivanović, Milan, Kuzmanović, Nemanja, Gašić, Katarina, Prokić, Andjelka, Zlatković, Nevena, Obradović, Aleksa, "Specificity and sensitivity of three pcr-based methods for detection of erwinia amylovora in pure culture and plant material" in Genetika, 51, no. 3 (2019):1039-1052,
https://doi.org/10.2298/GENSR1903039I . .

TY  - JOUR
AU  - Krivokapić, Marija
AU  - Gavrilović, Veljko
AU  - Ivanović, Milan
AU  - Kuzmanović, Nemanja
AU  - Fira, Đorđe
AU  - Obradović, Aleksa
AU  - Gašić, Katarina
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/545
AB  - The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation.
AB  - Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia
T1  - Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji
EP  - 184
IS  - 3-4
SP  - 175
VL  - 33
DO  - 10.2298/PIF1804175K
ER  - 

@article{
author = "Krivokapić, Marija and Gavrilović, Veljko and Ivanović, Milan and Kuzmanović, Nemanja and Fira, Đorđe and Obradović, Aleksa and Gašić, Katarina",
year = "2018",
abstract = "The diversity of 30 Erwinia amylovora strains, isolated from quince, pear and apple trees on 14 localities in Serbia, was studied using bacteriological and molecular methods. In pathogenicity tests, all strains caused necrosis and oozing of bacterial exudate on inoculated immature pear, cherry and plum fruits, and induced hypersensitive reaction in tobacco leaves. The studied strains were Gram and oxidase negative, non-fluorescent, levan and catalase positive and facultatively anaerobic. The strains did not reduce nitrates, but utilized citrate and produced acid from sorbitol, hydrolyzed gelatine, produced reducing substances from sucrose and grew in the presence of 5% NaCl, but not at 36oC. Identity of the strains was confirmed by conventional and nested PCR methods. Rep-PCR with REP, ERIC and BOX primers resulted in amplification of several DNA fragments respectively, but showed no variation within the strains. However, different genetic profiles were obtained with RAPD-PCR by using six primers which enabled differentiation of the strains into four groups. Genetic differences between the studied strains did not correlate with the host plants, geographical origin or year of isolation., Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdvajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia, Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji",
pages = "184-175",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804175K"
}

Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, Đ., Obradović, A.,& Gašić, K.. (2018). Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 175-184.
https://doi.org/10.2298/PIF1804175K

Krivokapić M, Gavrilović V, Ivanović M, Kuzmanović N, Fira Đ, Obradović A, Gašić K. Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia. in Pesticidi i fitomedicina. 2018;33(3-4):175-184.
doi:10.2298/PIF1804175K .

Krivokapić, Marija, Gavrilović, Veljko, Ivanović, Milan, Kuzmanović, Nemanja, Fira, Đorđe, Obradović, Aleksa, Gašić, Katarina, "Characterization and population diversity of Erwinia amylovora strains originating from pome fruits in Serbia" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):175-184,
https://doi.org/10.2298/PIF1804175K . .

TY  - JOUR
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/508
AB  - Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.
PB  - Frontiers Media S.A.
T2  - Frontiers In Microbiology
T1  - Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot
VL  - 9
DO  - 10.3389/fmicb.2018.02021
ER  - 

@article{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Ivanović, Milan and Prokić, Anđelka and Šević, Milan and Obradović, Aleksa",
year = "2018",
abstract = "Xanthomonas euvesicatoria phage K Phi 1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of K Phi D1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage K Phi 1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted K Phi 1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to K Phi 1. Phage K Phi 1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4 degrees C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental to the phage strain, but skim milk plus sucrose formulation extended its survival in vitro. The phages survived for at least 7 days on the surface of pepper leaves in the greenhouse, showing the ability to persist on the plant tissue without the presence of the host bacterium. Results of three repeated experiments showed that foliar applications of the unformulated K Phi 1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. The integration of the phage K Phi 1 and copper-hydroxide treatments resulted in an increased efficacy compared to the copper-hydroxide alone.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers In Microbiology",
title = "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot",
volume = "9",
doi = "10.3389/fmicb.2018.02021"
}

Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M.,& Obradović, A.. (2018). Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers In Microbiology
Frontiers Media S.A.., 9.
https://doi.org/10.3389/fmicb.2018.02021

Gašić K, Kuzmanović N, Ivanović M, Prokić A, Šević M, Obradović A. Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot. in Frontiers In Microbiology. 2018;9.
doi:10.3389/fmicb.2018.02021 .

Gašić, Katarina, Kuzmanović, Nemanja, Ivanović, Milan, Prokić, Anđelka, Šević, Milan, Obradović, Aleksa, "Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage K Phi 1, Its Survival and Potential in Control of Pepper Bacterial Spot" in Frontiers In Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.02021 . .

TY  - CONF
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/522
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot
EP  - 133
IS  - 10
SP  - 132
VL  - 108
ER  - 

@conference{
author = "Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, M. and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot",
pages = "133-132",
number = "10",
volume = "108"
}

Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zlatković, N.,& Obradović, A.. (2018). Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 132-133.

Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zlatković N, Obradović A. Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot. in Phytopathology. 2018;108(10):132-133..

Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, M., Zlatković, Nevena, Obradović, Aleksa, "Biocontrol potential of bacteriophage K Phi 1 in control of pepper bacterial spot" in Phytopathology, 108, no. 10 (2018):132-133.

TY  - CONF
AU  - Prokić, Anđelka
AU  - Ivanović, M.
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Obradović, Aleksa
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/521
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro
EP  - 24
IS  - 10
SP  - 24
VL  - 108
ER  - 

@conference{
author = "Prokić, Anđelka and Ivanović, M. and Gašić, Katarina and Kuzmanović, Nemanja and Zlatković, Nevena and Obradović, Aleksa",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro",
pages = "24-24",
number = "10",
volume = "108"
}

Prokić, A., Ivanović, M., Gašić, K., Kuzmanović, N., Zlatković, N.,& Obradović, A.. (2018). Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 24-24.

Prokić A, Ivanović M, Gašić K, Kuzmanović N, Zlatković N, Obradović A. Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro. in Phytopathology. 2018;108(10):24-24..

Prokić, Anđelka, Ivanović, M., Gašić, Katarina, Kuzmanović, Nemanja, Zlatković, Nevena, Obradović, Aleksa, "Studying Xanthomonas arboricola pv. corylina strains from Serbia for streptomycin and kasugamycin resistance and copper sulfate sensitivity in vitro" in Phytopathology, 108, no. 10 (2018):24-24.

TY  - JOUR
AU  - Jerinić Prodanović, Dušanka
AU  - Obradović, Aleksa
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Zlatković, Nevena
AU  - Pavlović, Žaklina
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1036
AB  - Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi)i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericeranigricornis Foerster, u morfološkom i bioekološkom smislu veoma sli nih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebaena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u pove anoj brojnosti mogu dovesti do hloroze i žu enja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvr ena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hloroti nog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvr ena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine prou avano je prisustvo, biologija i štetnost B. trigonicana podru ju Srbije. Prvi put je registrovana 2014. godine i od tada je utvr eno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima ve i broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.
PB  - •	Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia
EP  - 384
IS  - 375
VL  - 45
VL  - 4
ER  - 

@article{
author = "Jerinić Prodanović, Dušanka and Obradović, Aleksa and Ivanović, Milan and Prokić, Anđelka and Zlatković, Nevena and Pavlović, Žaklina",
year = "2017",
abstract = "Mrkvina lisna buva Bactericera trigonica pripada familiji Triozidae, nadfamiliji Psylloidea, redu Hemiptera. Zajedno sa lukovom lisnom buvom (B. tremblayi)i krompirovom lisnom buvom (B. nigricornis) pripada kompleksu Bactericeranigricornis Foerster, u morfološkom i bioekološkom smislu veoma sli nih vrsta. Opisana je 1981. godine kao Trioza trigonica na osnovu analize imaga sakupljanih sa mrkve iz Portugalije, Italije, Kipra, Turske, Egipta i Irana. Kasnije je prebaena u rod Bactericera zajedno sa druge dve vrste ovog kompleksa. Imaga i larve se hrane na listu mrkve i drugih štitonoša. Ukoliko se jave u pove anoj brojnosti mogu dovesti do hloroze i žu enja lista, što ima za posledicu smanjenje prinosa. Pored direktnih šteta, utvr ena je njena vektorska uloga fastidiozne bakterije «Candidatus Liberibacter solanacearum», koja na biljkama iz familije Apiaceae dovodi do proliferacije korena i hloroti nog slabljenja. Ca. L. solanacearum je odavno poznata u Severnoj Americi i Novom Zelandu na biljkama iz familije Solanacae, prevashodno na krompiru, a u Evropi je prvi put utvr ena na mrkvi i celeru, a B. trigonica kao njen vektor. U periodu od 2014. do 2017. godine prou avano je prisustvo, biologija i štetnost B. trigonicana podru ju Srbije. Prvi put je registrovana 2014. godine i od tada je utvr eno njeno prisustvo u svim lokalitetima istraživanja tokom celog vegetacionog perioda mrkve. Pored mrkve, B. trigonica je registrovana na celeru, peršunu i divljoj mrkvi. U toku godine ima ve i broj generacija, prezimljava u stadijumu imaga na skrovitim mestima.",
publisher = "•	Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia",
pages = "384",
number = "375",
volume = "45, 4"
}

Jerinić Prodanović, D., Obradović, A., Ivanović, M., Prokić, A., Zlatković, N.,& Pavlović, Ž.. (2017). Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar
•	Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(375).

Jerinić Prodanović D, Obradović A, Ivanović M, Prokić A, Zlatković N, Pavlović Ž. Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia. in Biljni lekar. 2017;45(375):null-384..

Jerinić Prodanović, Dušanka, Obradović, Aleksa, Ivanović, Milan, Prokić, Anđelka, Zlatković, Nevena, Pavlović, Žaklina, "Distribution, biology and harmfulness of carrot psyllid Bactericera trigonica (Hodkinson, 1981) (Hemiptera, Triozidae) in Serbia" in Biljni lekar, 45, no. 375 (2017).

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Šević, Milan
AU  - Obradović, Aleksa
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/504
AB  - Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents.
AB  - Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.
PB  - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Distribution and significance of causal agents of bacterial spot of pepper and tomato
T1  - Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza
EP  - 596
IS  - 6
SP  - 587
VL  - 45
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Šević, Milan and Obradović, Aleksa",
year = "2017",
abstract = "Bacterial spot of pepper and tomato regularly causes losses in production in Serbia. According to the new systematization, four Xanthomonas species have been reported as causal agents of leaf spots of pepper and tomato: X. euvesicatoria, X. vesicatoria, X. perforans and X. gardneri. Due to wide distribution and great damage at the global level, X. euvesicatoria is considered one of the most significant parasitic bacteria of pepper. Causal agent of the bacterial spot on tomato in Serbia is X. vesicatoria. Occurrence of of both species is observed every year under Serbian agroecological conditions, mainly due to the cultivation of susceptible assortment and conditions which are suitable for disease development. The disease is seed transmitted and it can cause defoliation and crop degradation if conditions suitable for its development occur. Bacterial spot of pepper and tomato cannot be combatted easily and application of several cultivation practices including conventional or microbial preparations - biopesticides is therefore required. The existence of natural antagonists (bacteriophage) isolated from soil has been confirmed, which could be used for biological control of pepper bacterial spot. In the absence of effective preventive measures, the solution should be sought within an integrated approach - the synthesis of knowledge about the biology and epidemiology of the pathogen, crop production technology, as well as bactericidal effect of some natural agents., Prema najnovijoj sistematici kao prouzrokovači bakteriozne pegavosti paprike i paradajza navode se četiri vrste roda Xanthomonas: X. euvesicatoria, X. vesicatoria, X. perforans i X. gardneri. Prema rasprostranjenosti i štetama koje nanosi, prouzrokovač bakteriozne pegavosti lišća i krastavosti plodova X. euvesicatoria spada u najznačajnije bakterije parazite paprike u svetu, dok se u agroekološkim uslovima Srbije, usled gajenja osetljivog sortimenta i povoljnih uslova za razvoj bolesti, pojavljuje svake godine u većem ili manjem intenzitetu. Kao prouzrokovač ovog oboljenja na paradajzu navodi se X. vesicatoria. Bakterije se prenose semenom i u povoljnim uslovima za razvoj bolesti, mogu prouzrokovati plamenjaču, potpunu defolijaciju i izumiranje biljaka. U zaštiti od bakteriozne pegavosti paprike i paradajza potrebno je preduzeti niz mera koje podrazumevaju korišćenje konvencionalnih, ali i bioloških preparata. Utvrđeno je postojanje prirodnih antagonista (bakteriofaga) izolovanih iz zemljišta koji se mogu koristiti u biološkoj borbi. U nedostatku efikasnih mera, rešenje treba tražiti u integralnom pristupu, odnosno sintezi saznanja o biologiji i epidemiologiji patogena, tehnologiji biljne proizvodnje, kao i baktericidnom efektu pojedinih prirodnih agenasa.",
publisher = "Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Distribution and significance of causal agents of bacterial spot of pepper and tomato, Rasprostranjenost i značaj prouzrokovača bakteriozne pegavosti lista i krastavosti plodova paprike i paradajza",
pages = "596-587",
number = "6",
volume = "45"
}

Ignjatov, M., Gašić, K., Šević, M.,& Obradović, A.. (2017). Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar
Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 587-596.

Ignjatov M, Gašić K, Šević M, Obradović A. Distribution and significance of causal agents of bacterial spot of pepper and tomato. in Biljni lekar. 2017;45(6):587-596..

Ignjatov, Maja, Gašić, Katarina, Šević, Milan, Obradović, Aleksa, "Distribution and significance of causal agents of bacterial spot of pepper and tomato" in Biljni lekar, 45, no. 6 (2017):587-596.